Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0010294

Physical Location & Seq

Genomic Coordinates Reverse (-)

15436971 .. 15446624

Gene Structure 9654 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00094120.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2469 bp

ATGGACAAGTCCAGCGCTCTCGAATACATCAACCAGATGTTCCCCACAGAGGCATCGTTATCCGGCGTCGAGCCGCTAATGCAGAAGATTCACAGTGAAATTCGGCGCGTTGATGCTGGAATTCTAGCTGCCGTTCGTCAACAGAGCAATTCAGGGACCAAAGCCAAGGAGGATCTTGCTGCTGCTACGCGAGCCGTGGAGGAACTCATGTATAAGATCCGGGAAATCAAAACAAAGGCTGAGCAGAGTGAGACAATGGTTCAGGAGATATGTCGTGACATTAAGAAGTTGGATTTTGCGAAGAAGCATATAACAACCACCATTACTGCTCTTCATCGTCTGACCATGCTCGTCTCTGCTGTTGAACAGCTTCAAGTGATGGCTTCCAAACGACAATATAAAGAGGCGGCTGCACAGTTGGAGGCTGTGAACCAATTATGTAGCCATTTTGAAGCCTATAGGGATGTTCCAAAAATCACAGAGCTCAGGGAGAAATTTAAGAATATCAAACAAATATTAAAGTCACACGTGTTTTCCGATTTCTCAAGCTTAGGTACTGGAAAGGAGTCAGAAGAATCTAATTTACTCCAGCAGCTATCTGAGGCTTGCTTAGTTGTTGATGCATTAGAGGCATCTGTGAGGGAGGAATTAGTTAATAACTTTTGCAGCAGGGAGCTTACTTCTTATGAGCAAATCTTTGAAGGAGCTGAACTAGCTAAGTTGGATAAGACTGAACGAAGATATGCATGGATCAAGCGCCGCATGAGAACGAATGAAGAAATCTGGAAAATCTTTCCTTCTTCATGGCATGTTTCTTATCGACTCTGCATTCAGTTCTGCAAAAAAACAAGGAAGCAGCTAGAAGATATCCTTAATAATCAGAAAGAAAAGCCAGATGTTGGGACATTGCTGATGGCACTACAGCGAACTCTTGAATTTGAGGATGAATTGGCAGAAAAATTTGGAGGTGGTACTCGAGGCAGGGAGGTTGGAAATGAGATTGAAGAAATTGGTAGAGGAGAAAACACTACTCAAAGTGCTTCAGATATTCGAAAGAAATATGAAAAGAAGCTTGCTGCCCATCAAGGAAATGCCACAGAGGAAAAGGATAAAGAGTTATCTGTGCCTGGAGCTGGGTTCAACTTCCGTGGAATCATCTCATCTTGCTTTGAGCCTCACTTGACTGTTTACACAGAACTAGAAGAGAAGACACTAATGGAGAATTTGGAAAAACTTGTTCAGGAGGAGACATGGGATGTTGAGGAGGGAAGTCAGAATAATGTTTTGTCGAGTAGCATGCAGTTGTTTCTTATAATCAAGAGGAGCTTAAAACGGTGCAGTGCTTTAACAAAAAATCAGACATTATTTAACTTGTTCAAGGTGTTTCAAAGGATTCTCAAAGCATATGCAACAAAGCTTTTTATAAGATTACCTAAAGGTGGTACAGGAATTGTTGCAGCAGCAACTGGTATGGATGGACAGATAAAGCAGACATCAGACAGAGATGAGAGGGTGATTTGTTACATTGTCAATTCAGCGGAATATTGCACCAACACGTCTGGTGAACTCGCTGAGAGTGTGTCCAAAATAATTGATTCTCAATTCGCAGATGGGGTGGATATGTCGGAAGTGCAGGATGAATTTTCAGCAGTTATAACAAGAGCATTGGTAACCTTGGTGCATGGCCTAGAAACCAAGTTTGACAATGAAATGGCTGCAATGACACGAGTTCCATGGGGTACACTTGAAAGTGTTGGTGACCAATCAGAGTATGTTAATGGCATAAATATGATCCTTGCTAGTAGTATACCTATACTAGGAAGCCTTCTTTCACCAATCTACTTCCAATTCTTCTTGGACAAGTTGGCATCATCTCTTGGTCCACGTTTCTATGCTAACATTTTCAAGTGCAAGCAAATATCAGAGACTGGAGCTCAACAGATGCTATTGGATACTCAAGCTGTAAAGACTATTCTTTTGGATATCCCATCACTTGGTCACCAGACGTCACGTGCTGCAAGCTATGCGAAATTTGTAAGCCGTGAGATGAGCAAAGCTGAAGCACTTCTAAAGGTCATACTGTCCCCAATTGATTCTGTCGCAGATACATATCGTGCATTGCTACCAGAGGGAACACCTATGGAGTTTCAGCGAATTCTGGAGCTCAAGGGTCTTAAAAAGGCAGATCAGCAGAGTATACTTGAAGACTTCAACAAGCATGGGCCGGGAATTACTCAACCTTCTACCCCACCACCACCAGTTGCCACACCTGTTCCAACTGCCCCGACAGTCTCTCTGATACAAATTCCCACTTCAGGTGGATTCCTTGCACCCAGAGAAGATGTACTAACCAGAGCAGCTGCACTTGGACGTGGTGCAGCCACAACAGGGTTCAAGAGGTTTCTAGCTTTAACAGAAGCTGCAAAGGACCGGAAAGATGGGCCTTTCAGGAAGCTCTTCAATGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0000938 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005794 GO:0006810 GO:0006890 GO:0006950 GO:0006970 GO:0007009 GO:0008150 GO:0009266 GO:0009408 GO:0009628 GO:0009987 GO:0010256 GO:0010286 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016197 GO:0016482 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0032991 GO:0042147 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044433 GO:0044444 GO:0044446 GO:0044464 GO:0046907 GO:0048193 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0061024 GO:0071840 GO:0097708 GO:0098588 GO:0098791 GO:0099023

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Vps53_N

Protein Analysis

822

Amino Acids

91.88

Weight (kDa)

6.51

Isoelectric Point (pI)

39.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Vps53_N	PF04100	3 - 416	5.4e-143	Vps53, N-terminal
VPS53_C	PF16854	647 - 725	9.5e-14	Vacuolar protein sorting-associated protein 53 C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010294)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G50500 AT1G50500 AT1G50500 AT1G50500 AT1G50500
fragaria_vesca	FvH4_6g40130 FvH4_6g40130
malus_domestica	MD09G1135000.v1.1 MD17G1124000.v1.1
prunus_persica	Prupe.3G191700_v2.0.a1
pyrus_communis	pycom09g05610 pycom17g11540
rosa_chinensis	RchiOBHm_Chr2g0154761
rosa_laevigata	RLG00000020801
rosa_multiflora	Rmu_sc0001027.1_g000002
rosa_roxburghii	Rroxscaffold_2G00094120
rosa_rugosa	Rorug02G0444800
rosa_samantha	Rh2BG521400 Rh2CG495700 Rh2DG531500
rosa_wichuraiana	Rw2G041990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	1313, 1424, 1655
AatII	GACGTC	1 cut(s)	2009
AccB7I	CCANNNNNTGG	2 cut(s)	899, 1994
AccI	GTMKAC	2 cut(s)	1807, 2197
AccII	CGCG	2 cut(s)	108, 190
AciI	CCGC	4 cut(s)	74, 407, 760, 1538
AclWI	GGATC	4 cut(s)	180, 211, 758, 1786
AcuI	CTGAAG	3 cut(s)	1026, 2079, 2298
AcvI	CACGTG	2 cut(s)	529, 2012
AcyI	GRCGYC	2 cut(s)	66, 2006
AfaI	GTAC	5 cut(s)	556, 973, 1444, 1741, 2346
AfeI	AGCGCT	1 cut(s)	16
AfiI	CCNNNNNNNGG	8 cut(s)	49, 899, 1133, 1439, 1817, 1994, 2315, 2388
AflIII	ACRYGT	3 cut(s)	526, 528, 1554
AjiI	CACGTC	2 cut(s)	1557, 2372
AjnI	CCWGG	1 cut(s)	1126
Alw21I	GWGCWC	3 cut(s)	486, 1936, 2166
Alw26I	GTCTC	5 cut(s)	245, 358, 1241, 1919, 2296
AlwI	GGATC	4 cut(s)	180, 211, 758, 1786
AlwNI	CAGNNNCTG	3 cut(s)	1466, 1928, 2420
Ama87I	CYCGRG	1 cut(s)	975
Aor51HI	AGCGCT	1 cut(s)	16
AoxI	GGCC	3 cut(s)	1684, 2222, 2441
ArsI	GACNNNNNNTTYG	2 cut(s)	1960, 1992
Asp700I	GAANNNNTTC	3 cut(s)	369, 2064, 2456
AspLEI	GCGC	3 cut(s)	17, 108, 759
AspS9I	GGNCC	5 cut(s)	156, 1880, 2222, 2428, 2441
AsuC2I	CCSGG	2 cut(s)	221, 2226
AsuHPI	GGTGA	5 cut(s)	1525, 1574, 1769, 1824, 1991
AsuII	TTCGAA	1 cut(s)	1051
AvaI	CYCGRG	1 cut(s)	975
AvaII	GGWCC	3 cut(s)	156, 1880, 2428
BanII	GRGCYC	3 cut(s)	486, 1936, 2166
BauI	CACGAG	1 cut(s)	1725
BbrPI	CACGTG	2 cut(s)	529, 2012
BbsI	GAAGAC	2 cut(s)	1214, 2211
Bbv12I	GWGCWC	3 cut(s)	486, 1936, 2166
BccI	CCATC	7 cut(s)	373, 907, 1089, 1469, 1604, 1996, 2432
BceAI	ACGGC	3 cut(s)	116, 179, 2025
BcgI	CGANNNNNNTGC	2 cut(s)	1279, 1313
BciT130I	CCWGG	1 cut(s)	1128
BciVI	GTATCC	1 cut(s)	1945
BcnI	CCSGG	2 cut(s)	221, 2226
BcoDI	GTCTC	5 cut(s)	245, 358, 1241, 1919, 2296
BfaI	CTAG	8 cut(s)	125, 713, 860, 1199, 1688, 1800, 1817, 2405
BfmI	CTRYAG	2 cut(s)	457, 920
BfoI	RGCGCY	2 cut(s)	18, 760
BfuI	GTATCC	1 cut(s)	1945
BlpI	GCTNAGC	1 cut(s)	240
Bme1390I	CCNGG	3 cut(s)	221, 1128, 2226
Bme18I	GGWCC	3 cut(s)	156, 1880, 2428
BmeT110I	CYCGRG	1 cut(s)	975
BmgBI	CACGTC	2 cut(s)	1557, 2372
BmgT120I	GGNCC	5 cut(s)	156, 1880, 2222, 2428, 2441
BmiI	GGNNCC	1 cut(s)	157
BmrFI	CCNGG	3 cut(s)	221, 1128, 2226
BmsI	GCATC	6 cut(s)	62, 103, 610, 641, 1877, 1932
BpiI	GAAGAC	2 cut(s)	1214, 2211
BpmI	CTGGAG	4 cut(s)	572, 1149, 1950, 2180
Bpu10I	CCTNAGC	2 cut(s)	485, 550
Bpu1102I	GCTNAGC	1 cut(s)	240
Bpu14I	TTCGAA	1 cut(s)	1051
BpuEI	CTTGAG	3 cut(s)	529, 1941, 2150
BpuMI	CCSGG	2 cut(s)	221, 2226
BsaAI	YACGTR	2 cut(s)	529, 2012
BsaBI	GATNNNNATC	1 cut(s)	2109
BsaHI	GRCGYC	2 cut(s)	66, 2006
BsaJI	CCNNGG	5 cut(s)	165, 195, 1147, 1674, 1733
BsaWI	WCCGGW	1 cut(s)	2430
Bsc4I	CCNNNNNNNGG	8 cut(s)	49, 899, 1133, 1439, 1817, 1994, 2315, 2388
Bse1I	ACTGG	4 cut(s)	562, 1471, 1933, 2258
Bse3DI	GCAATG	3 cut(s)	905, 1725, 2117
Bse8I	GATNNNNATC	1 cut(s)	2109
BseBI	CCWGG	1 cut(s)	1128
BseDI	CCNNGG	5 cut(s)	165, 195, 1147, 1674, 1733
BseGI	GGATG	5 cut(s)	469, 949, 1261, 1480, 1642
BseJI	GATNNNNATC	1 cut(s)	2109
BseLI	CCNNNNNNNGG	8 cut(s)	49, 899, 1133, 1439, 1817, 1994, 2315, 2388
BseMI	GCAATG	3 cut(s)	905, 1725, 2117
BseMII	CTCAG	4 cut(s)	231, 499, 591, 1563
BseNI	ACTGG	4 cut(s)	562, 1471, 1933, 2258
BseRI	GAGGAG	4 cut(s)	1032, 1259, 1277, 1336
BseYI	CCCAGC	1 cut(s)	1133
BsgI	GTGCAG	5 cut(s)	396, 1357, 1652, 2346, 2397
Bsh1236I	CGCG	2 cut(s)	108, 190
BshFI	GGCC	3 cut(s)	1686, 2224, 2443
BsiHKAI	GWGCWC	3 cut(s)	486, 1936, 2166
BsiHKCI	CYCGRG	1 cut(s)	975
BsiSI	CCGG	4 cut(s)	63, 220, 2225, 2431
BslFI	GGGAC	3 cut(s)	169, 916, 2068
BslI	CCNNNNNNNGG	8 cut(s)	49, 899, 1133, 1439, 1817, 1994, 2315, 2388
BsmAI	GTCTC	5 cut(s)	245, 358, 1241, 1919, 2296
BsmBI	CGTCTC	1 cut(s)	358
BsmFI	GGGAC	3 cut(s)	169, 916, 2068
BsmI	GAATGC	1 cut(s)	828
BsnI	GGCC	3 cut(s)	1686, 2224, 2443
BsoBI	CYCGRG	1 cut(s)	975
Bsp119I	TTCGAA	1 cut(s)	1051
Bsp1286I	GDGCHC	3 cut(s)	486, 1936, 2166
Bsp143I	GATC	5 cut(s)	172, 216, 750, 1791, 2185
Bsp1720I	GCTNAGC	1 cut(s)	240
Bsp19I	CCATGG	1 cut(s)	1733
BspACI	CCGC	4 cut(s)	74, 407, 760, 1538
BspANI	GGCC	3 cut(s)	1686, 2224, 2443
BspCNI	CTCAG	4 cut(s)	232, 498, 592, 1564
BspFNI	CGCG	2 cut(s)	108, 190
BspLI	GGNNCC	1 cut(s)	157
BspPI	GGATC	4 cut(s)	180, 211, 758, 1786
BspQI	GCTCTTC	2 cut(s)	336, 2462
BspT104I	TTCGAA	1 cut(s)	1051
BsrDI	GCAATG	3 cut(s)	905, 1725, 2117
BsrI	ACTGG	4 cut(s)	562, 1471, 1933, 2258
BssECI	CCNNGG	5 cut(s)	165, 195, 1147, 1674, 1733
BssMI	GATC	5 cut(s)	172, 216, 750, 1791, 2185
BssNAI	GTATAC	2 cut(s)	1808, 2198
BssNI	GRCGYC	2 cut(s)	66, 2006
BssSI	CACGAG	1 cut(s)	1725
BssT1I	CCWWGG	3 cut(s)	165, 1674, 1733
Bst1107I	GTATAC	2 cut(s)	1808, 2198
Bst2BI	CACGAG	1 cut(s)	1725
Bst2UI	CCWGG	1 cut(s)	1128
Bst4CI	ACNGT	6 cut(s)	95, 417, 1186, 1335, 2082, 2290
Bst6I	CTCTTC	3 cut(s)	336, 1197, 2462
BstACI	GRCGYC	2 cut(s)	66, 2006
BstAPI	GCANNNNNTGC	1 cut(s)	2024
BstBAI	YACGTR	2 cut(s)	529, 2012
BstBI	TTCGAA	1 cut(s)	1051
BstC8I	GCNNGC	6 cut(s)	192, 607, 1074, 1298, 1913, 2020
BstDEI	CTNAG	7 cut(s)	240, 485, 550, 600, 610, 717, 1572
BstDSI	CCRYGG	3 cut(s)	195, 1147, 1733
BstEII	GGTNACC	3 cut(s)	1669, 1757, 1997
BstENI	CCTNNNNNAGG	1 cut(s)	1815
BstF5I	GGATG	5 cut(s)	469, 949, 1261, 1480, 1642
BstFNI	CGCG	2 cut(s)	108, 190
BstH2I	RGCGCY	2 cut(s)	18, 760
BstHHI	GCGC	3 cut(s)	17, 108, 759
BstKTI	GATC	5 cut(s)	175, 219, 753, 1794, 2188
BstMAI	GTCTC	5 cut(s)	245, 358, 1241, 1919, 2296
BstMBI	GATC	5 cut(s)	172, 216, 750, 1791, 2185
BstMWI	GCNNNNNNNGC	5 cut(s)	79, 191, 629, 713, 2024
BstNI	CCWGG	1 cut(s)	1128
BstNSI	RCATGY	2 cut(s)	812, 1300
BstPI	GGTNACC	3 cut(s)	1669, 1757, 1997
BstSCI	CCNGG	3 cut(s)	219, 1126, 2224
BstSFI	CTRYAG	2 cut(s)	457, 920
BstUI	CGCG	2 cut(s)	108, 190
BstV2I	GAAGAC	2 cut(s)	1214, 2211
BstX2I	RGATCY	2 cut(s)	172, 216
BstYI	RGATCY	2 cut(s)	172, 216
BstZ17I	GTATAC	2 cut(s)	1808, 2198
BsuI	GTATCC	1 cut(s)	1945
BsuRI	GGCC	3 cut(s)	1686, 2224, 2443
BtgI	CCRYGG	3 cut(s)	195, 1147, 1733
BtrI	CACGTC	2 cut(s)	1557, 2372
BtsCI	GGATG	5 cut(s)	469, 949, 1261, 1480, 1642
BtsI	GCAGTG	1 cut(s)	1345
BtsIMutI	CAGTG	2 cut(s)	100, 1345
Cac8I	GCNNGC	6 cut(s)	192, 607, 1074, 1298, 1913, 2020
CaiI	CAGNNNCTG	3 cut(s)	1466, 1928, 2420
CfoI	GCGC	3 cut(s)	17, 108, 759
Cfr13I	GGNCC	5 cut(s)	156, 1880, 2222, 2428, 2441
CseI	GACGC	1 cut(s)	55
Csp6I	GTAC	5 cut(s)	555, 972, 1443, 1740, 2345
CspCI	CAANNNNNGTGG	2 cut(s)	1129, 1164
CviQI	GTAC	5 cut(s)	555, 972, 1443, 1740, 2345
DdeI	CTNAG	7 cut(s)	240, 485, 550, 600, 610, 717, 1572
DpnI	GATC	5 cut(s)	174, 218, 752, 1793, 2187
DpnII	GATC	5 cut(s)	172, 216, 750, 1791, 2185
Eam1104I	CTCTTC	3 cut(s)	336, 1197, 2462
EarI	CTCTTC	3 cut(s)	336, 1197, 2462
Ecl136II	GAGCTC	3 cut(s)	484, 1934, 2164
Eco130I	CCWWGG	3 cut(s)	165, 1674, 1733
Eco24I	GRGCYC	3 cut(s)	486, 1936, 2166
Eco32I	GATATC	2 cut(s)	868, 1984
Eco47I	GGWCC	3 cut(s)	156, 1880, 2428
Eco47III	AGCGCT	1 cut(s)	16
Eco53kI	GAGCTC	3 cut(s)	484, 1934, 2164
Eco57I	CTGAAG	3 cut(s)	1026, 2079, 2298
Eco72I	CACGTG	2 cut(s)	529, 2012
Eco88I	CYCGRG	1 cut(s)	975
Eco91I	GGTNACC	3 cut(s)	1669, 1757, 1997
EcoICRI	GAGCTC	3 cut(s)	484, 1934, 2164
EcoNI	CCTNNNNNAGG	1 cut(s)	1815
EcoO65I	GGTNACC	3 cut(s)	1669, 1757, 1997
EcoRI	GAATTC	2 cut(s)	120, 2154
EcoRII	CCWGG	1 cut(s)	1126
EcoRV	GATATC	2 cut(s)	868, 1984
EcoT14I	CCWWGG	3 cut(s)	165, 1674, 1733
EcoT22I	ATGCAT	2 cut(s)	625, 748
EcoT38I	GRGCYC	3 cut(s)	486, 1936, 2166
ErhI	CCWWGG	3 cut(s)	165, 1674, 1733
Esp3I	CGTCTC	1 cut(s)	358
FalI	AAGNNNNNCTT	4 cut(s)	855, 887, 1310, 1342
FaqI	GGGAC	3 cut(s)	169, 916, 2068
FauNDI	CATATG	1 cut(s)	1405
FblI	GTMKAC	2 cut(s)	1807, 2197
FokI	GGATG	5 cut(s)	476, 956, 1268, 1487, 1649
FriOI	GRGCYC	3 cut(s)	486, 1936, 2166
FspBI	CTAG	8 cut(s)	125, 713, 860, 1199, 1688, 1800, 1817, 2405
GlaI	GCGC	3 cut(s)	16, 107, 758
GsaI	CCCAGC	1 cut(s)	1137
GsuI	CTGGAG	4 cut(s)	572, 1149, 1950, 2180
HaeII	RGCGCY	2 cut(s)	18, 760
HaeIII	GGCC	3 cut(s)	1686, 2224, 2443
HapII	CCGG	4 cut(s)	63, 220, 2225, 2431
HgaI	GACGC	1 cut(s)	55
HhaI	GCGC	3 cut(s)	17, 108, 759
Hin1I	GRCGYC	2 cut(s)	66, 2006
Hin6I	GCGC	3 cut(s)	15, 106, 757
HinP1I	GCGC	3 cut(s)	15, 106, 757
HincII	GTYRAC	1 cut(s)	140
HindII	GTYRAC	1 cut(s)	140
HindIII	AAGCTT	3 cut(s)	547, 1070, 1415
HinfI	GANTC	9 cut(s)	88, 566, 575, 822, 1152, 1393, 1595, 2093, 2322
HpaII	CCGG	4 cut(s)	63, 220, 2225, 2431
HphI	GGTGA	5 cut(s)	1525, 1574, 1769, 1824, 1991
Hpy166II	GTNNAC	8 cut(s)	140, 430, 1189, 1565, 1742, 1808, 1883, 2198
Hpy8I	GTNNAC	8 cut(s)	140, 430, 1189, 1565, 1742, 1808, 1883, 2198
Hpy99I	CGWCG	1 cut(s)	71
HpyAV	CCTTC	4 cut(s)	695, 807, 1835, 2250
HpyCH4III	ACNGT	6 cut(s)	95, 417, 1186, 1335, 2082, 2290
HpyCH4IV	ACGT	6 cut(s)	528, 1556, 1885, 2006, 2011, 2371
HpyF10VI	GCNNNNNNNGC	5 cut(s)	79, 191, 629, 713, 2024
HpyF3I	CTNAG	7 cut(s)	240, 485, 550, 600, 610, 717, 1572
HpySE526I	ACGT	6 cut(s)	528, 1556, 1885, 2006, 2011, 2371
Hsp92I	GRCGYC	2 cut(s)	66, 2006
HspAI	GCGC	3 cut(s)	15, 106, 757
Kzo9I	GATC	5 cut(s)	172, 216, 750, 1791, 2185
LguI	GCTCTTC	2 cut(s)	336, 2462
LmnI	GCTCC	6 cut(s)	673, 704, 1130, 1323, 1931, 2161
LweI	GCATC	6 cut(s)	62, 103, 610, 641, 1877, 1932
MaeI	CTAG	8 cut(s)	125, 713, 860, 1199, 1688, 1800, 1817, 2405
MaeII	ACGT	6 cut(s)	528, 1556, 1885, 2006, 2011, 2371
MaeIII	GTNAC	7 cut(s)	275, 522, 1520, 1669, 1757, 1997, 2007
MalI	GATC	5 cut(s)	174, 218, 752, 1793, 2187
MboI	GATC	5 cut(s)	172, 216, 750, 1791, 2185
MfeI	CAATTG	1 cut(s)	2088
MflI	RGATCY	2 cut(s)	172, 216
MhlI	GDGCHC	3 cut(s)	486, 1936, 2166
MlyI	GAGTC	2 cut(s)	575, 816
MmeI	TCCRAC	6 cut(s)	270, 399, 702, 970, 1605, 2300
Mph1103I	ATGCAT	2 cut(s)	625, 748
MroXI	GAANNNNTTC	3 cut(s)	369, 2064, 2456
MslI	CAYNNNNRTG	1 cut(s)	1787
MspA1I	CMGCKG	2 cut(s)	1538, 2360
MspI	CCGG	4 cut(s)	63, 220, 2225, 2431
MspR9I	CCNGG	3 cut(s)	221, 1128, 2226
MunI	CAATTG	1 cut(s)	2088
Mva1269I	GAATGC	1 cut(s)	828
MvaI	CCWGG	1 cut(s)	1128
MvnI	CGCG	2 cut(s)	108, 190
MwoI	GCNNNNNNNGC	5 cut(s)	79, 191, 629, 713, 2024
NciI	CCSGG	2 cut(s)	221, 2226
NcoI	CCATGG	1 cut(s)	1733
NdeI	CATATG	1 cut(s)	1405
NdeII	GATC	5 cut(s)	172, 216, 750, 1791, 2185
NlaIV	GGNNCC	1 cut(s)	157
NmuCI	GTSAC	5 cut(s)	275, 522, 1757, 1997, 2007
NsiI	ATGCAT	2 cut(s)	625, 748
NspI	RCATGY	2 cut(s)	812, 1300
NspV	TTCGAA	1 cut(s)	1051
PaeI	GCATGC	1 cut(s)	1300
PaeR7I	CTCGAG	1 cut(s)	975
PciSI	GCTCTTC	2 cut(s)	336, 2462
PcsI	WCGNNNNNNNCGW	1 cut(s)	534
PctI	GAATGC	1 cut(s)	828
PdmI	GAANNNNTTC	3 cut(s)	369, 2064, 2456
PfeI	GAWTC	7 cut(s)	88, 575, 1152, 1393, 1595, 2093, 2322
PflMI	CCANNNNNTGG	2 cut(s)	899, 1994
PfoI	TCCNGGA	1 cut(s)	219
PleI	GAGTC	2 cut(s)	574, 816
PmaCI	CACGTG	2 cut(s)	529, 2012
PmlI	CACGTG	2 cut(s)	529, 2012
PpsI	GAGTC	2 cut(s)	574, 816
Ppu21I	YACGTR	2 cut(s)	529, 2012
PsiI	TTATAA	3 cut(s)	1313, 1424, 1655
Psp124BI	GAGCTC	3 cut(s)	486, 1936, 2166
Psp6I	CCWGG	1 cut(s)	1126
PspCI	CACGTG	2 cut(s)	529, 2012
PspEI	GGTNACC	3 cut(s)	1669, 1757, 1997
PspFI	CCCAGC	1 cut(s)	1133
PspGI	CCWGG	1 cut(s)	1126
PspN4I	GGNNCC	1 cut(s)	157
PspPI	GGNCC	5 cut(s)	156, 1880, 2222, 2428, 2441
PspXI	VCTCGAGB	1 cut(s)	975
PstNI	CAGNNNCTG	3 cut(s)	1466, 1928, 2420
PsuI	RGATCY	2 cut(s)	172, 216
PvuII	CAGCTG	1 cut(s)	2360
RsaI	GTAC	5 cut(s)	556, 973, 1444, 1741, 2346
RsaNI	GTAC	5 cut(s)	555, 972, 1443, 1740, 2345
RseI	CAYNNNNRTG	1 cut(s)	1787
SacI	GAGCTC	3 cut(s)	486, 1936, 2166
SapI	GCTCTTC	2 cut(s)	336, 2462
Sau3AI	GATC	5 cut(s)	172, 216, 750, 1791, 2185
Sau96I	GGNCC	5 cut(s)	156, 1880, 2222, 2428, 2441
SchI	GAGTC	2 cut(s)	575, 816
ScrFI	CCNGG	3 cut(s)	221, 1128, 2226
SduI	GDGCHC	3 cut(s)	486, 1936, 2166
SfaNI	GCATC	6 cut(s)	62, 103, 610, 641, 1877, 1932
SfcI	CTRYAG	2 cut(s)	457, 920
Sfr274I	CTCGAG	1 cut(s)	975
SfuI	TTCGAA	1 cut(s)	1051
SinI	GGWCC	3 cut(s)	156, 1880, 2428
SlaI	CTCGAG	1 cut(s)	975
SmiMI	CAYNNNNRTG	1 cut(s)	1787
SmlI	CTYRAG	4 cut(s)	544, 975, 1956, 2165
SmoI	CTYRAG	4 cut(s)	544, 975, 1956, 2165
SphI	GCATGC	1 cut(s)	1300
SsiI	CCGC	4 cut(s)	74, 407, 760, 1538
SspI	AATATT	2 cut(s)	516, 1544
SspMI	CTAG	8 cut(s)	125, 713, 860, 1199, 1688, 1800, 1817, 2405
SstI	GAGCTC	3 cut(s)	486, 1936, 2166
StyD4I	CCNGG	3 cut(s)	219, 1126, 2224
StyI	CCWWGG	3 cut(s)	165, 1674, 1733
TaaI	ACNGT	6 cut(s)	95, 417, 1186, 1335, 2082, 2290
TaiI	ACGT	6 cut(s)	531, 1559, 1888, 2009, 2014, 2374
TaqI	TCGA	6 cut(s)	21, 69, 820, 976, 1051, 1289
TatI	WGTACW	1 cut(s)	2344
TauI	GCSGC	3 cut(s)	76, 410, 762
TfiI	GAWTC	7 cut(s)	88, 575, 1152, 1393, 1595, 2093, 2322
TscAI	CASTG	2 cut(s)	100, 1345
TseFI	GTSAC	5 cut(s)	275, 522, 1757, 1997, 2007
Tsp45I	GTSAC	5 cut(s)	275, 522, 1757, 1997, 2007
TspDTI	ATGAA	7 cut(s)	323, 789, 792, 960, 1077, 1653, 1722
TspGWI	ACGGA	1 cut(s)	1136
TspRI	CASTG	2 cut(s)	100, 1345
Van91I	CCANNNNNTGG	2 cut(s)	899, 1994
VpaK11BI	GGWCC	3 cut(s)	156, 1880, 2428
XagI	CCTNNNNNAGG	1 cut(s)	1815
XceI	RCATGY	2 cut(s)	812, 1300
XhoI	CTCGAG	1 cut(s)	975
XmiI	GTMKAC	2 cut(s)	1807, 2197
XmnI	GAANNNNTTC	3 cut(s)	369, 2064, 2456
XspI	CTAG	8 cut(s)	125, 713, 860, 1199, 1688, 1800, 1817, 2405
ZraI	GACGTC	1 cut(s)	2007
Zsp2I	ATGCAT	2 cut(s)	625, 748

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.