Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0010294

Physical Location & Seq

Genomic Coordinates Forward (+)

31688821 .. 31698548

Gene Structure 9728 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g40130.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2460 bp

ATGGACAAGTCCAGCGCTCTCGAATACATCAACCAGATGTTCCCCACAGAGGCGTCGTTGTCCGGCGTCGAGCCGCTAATGCAGAAGATTCACAGTGAGATTCGCCGCGTTGATGCCGGAATTCTAGCTGCCGTTCGTCAACAGAGCAATTCAGGGACCAAGGCTAAGGAGGATCTTGCTGCCGCAACGCGAGCCGTGAAGGAACTCATGTATAAGATCAGGGAAATCAAAACGAAGGCTGAGCAGAGTGAGACTATGGTTCAGGAGATATGTCGCGACATTAAGAAGTTGGACTTTGCGAAGAAGCATATAACGACTACCATCACTGCACTTCATCGTCTGACAATGCTTGTCTCTGCTGTTGAACAGCTTCAAGTGATGGCTTCCAAACGACAATATAAAGAGGCAGCTGCACAGTTGGAGGCTGTGAACCAGCTATGTAGCCATTTTGAAGCCTATAGGGATGTTCCGAAAATAACAGAACTCAGGGAGAAATTTAAGAATATCAAACAAATATTAAAGTCACACGTGTTTTCCGATTTCTCAAGCTTAGGTACCGGAAAGGAATCAGAGGAAAGTAACTTACTCCAGCAGCTATCTGAGGCTTGCTTGGTTGTTGATGCATTAGAGGCATCTGTGAGGGAAGAATTAGTAAATAACTTTTGCAGCAGGGAGCTTACTTCTTATGAGCAGATATTTGAAGGAGCTGAATTAGCGAAGTTGGATAAGACTGAACGACGATATGCATGGATCAAGCGCCGCATGAGAACGAATGAAGAAATCTGGACAATCTTTCCTTCTTCATGGCATGTATCTTATCGACTCTGCATTCAGTTCTGCAAAAAAACAAGGAAGCAGCTTGAAGATATCCTTAATTATCAGAAAGAAAAGCCAGATGTTGGGACATTACTGTTGGCACTACAGCGAACTCTTGAATTTGAGGATGAATTGGCAGAAAAATTCGGAGGTGGTACTCGAGGCAGGGAGGTTGCAAATGAGATTGAAGAAATTGGTAGAGAAAATACTACTCAAAATGCTTCAGATATTCGAAAGAAATATGAAAAGAAGTTTGCTGCCCATCAAGGAAATGCCACAGAGGAAAAGGATAAAGAGTTATCGGTGCCTGGAGCTGGGTTCAACTTCCGTGGAATCATCTCATCTTGCTTTGAGCCTCACTTGACTGTTTACACAGAACTAGAAGAGAAGACATTAATGGAGAATTTGGAGAAACTTGTTCAGGAGGAAACATGGGATGTTGAGGAGGGAAGTCAGAGTAGTGTTTTGTCAAGTAGCATGCAGTTGTTTCTTATTATCAAGAGAAGCTTAAAGCGATGCAGTGCTTTAACAAAGAACCAGACATTATTTAACTTGTTCAAGGTGTTTCAAAGGGTTCTCAAAGCATATGCAACAAAGCTGTTTGCTAGATTACCTAAAGGGGGTACAGGAATTGTTGCAGCAGCGACTGGTATGGATGGACAGATAAAGACATCAGACAGAGATGAAAGGGTGATCTGTTATATTGTCAATTCAGCTGAATATTGCACTAACACGTCTGGTGAACTCGCTGAGAGTGTGTCCAAAATAATCGATTCTCAATTGGCAGATGGGGTGGATATGTCGGAAGTGCAGGATGAATTTTCAGCAGTTATAACAAGAGCATTGGTAACCTTGGTGCAAGGCCTAGAAACCAAGTTTGACAATGAAATGGCTGCAATGACACGAGTTCCATGGGGTACACTTGAGAGTGTTGGTGACCAATCAGAATATGTTAATGGCATAAATATGATCCTTGCTAGTAGTATACCTATACTTGGAAGCCTTCTTTCACCGATCTACTTCCAGTTCTTCTTGGACAAGCTGGCATCATCTCTTGGTCCACGTTTCTATGCTAACATTTTCAAGTGCAAGCAAATATCAGAGACTGGAGCTCAACAAATGCTATTGGATACTCAAGCTGTAAAGACTATTCTTTTGGACATCCCATCACTTGGTCACCAGACTTCTCGTGCTGCAAGCTATGCGAAATTTGTAAGCCGTGAGATGAGCAAAGCTGAAGCACTTCTAAAGGTCATACTGTCCCCAATTGATTCTGTCGCAGATACATATCGTGCATTGCTACCAGAGGGAACACCTATGGAGTTTCAGCGAATTCTGGAACTCAAGGGTCTTAAAAAGGCAGATCAGCAGAGTATACTTGAAGACTTTAACAAGCATGGCCCAGGAATCACAAAACCTTCTGCCCCACCCCCGGTTGCGACACCTGTCCCAACTGCCCCGACAGTCTCTCTGATACAAAATCCCACTTCAGTTGGATTCCTTGCACCCAGAGAAGATGTACTAACCAGAGCAGCTGCACTTGGACGTGGTGCAGCCACAACAGGGTTCAAGAGATTCCTAGCTTTAACAGAAGCTGCGAAAGACCGGAAAGATGGGCCTTTCAGGAAGCTCTTCAATGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0000938 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005794 GO:0006810 GO:0006890 GO:0006950 GO:0006970 GO:0007009 GO:0008150 GO:0009266 GO:0009408 GO:0009628 GO:0009987 GO:0010256 GO:0010286 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016197 GO:0016482 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0032991 GO:0042147 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044433 GO:0044444 GO:0044446 GO:0044464 GO:0046907 GO:0048193 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0061024 GO:0071840 GO:0097708 GO:0098588 GO:0098791 GO:0099023

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Vps53_N

Protein Analysis

820

Amino Acids

91.54

Weight (kDa)

6.85

Isoelectric Point (pI)

38.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Vps53_N	PF04100	2 - 159	2.5e-35	Vps53, N-terminal
VPS53_C	PF16854	389 - 467	5.6e-14	Vacuolar protein sorting-associated protein 53 C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010294)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G50500 AT1G50500 AT1G50500 AT1G50500 AT1G50500
fragaria_vesca	FvH4_6g40130 FvH4_6g40130
malus_domestica	MD09G1135000.v1.1 MD17G1124000.v1.1
prunus_persica	Prupe.3G191700_v2.0.a1
pyrus_communis	pycom09g05610 pycom17g11540
rosa_chinensis	RchiOBHm_Chr2g0154761
rosa_laevigata	RLG00000020801
rosa_multiflora	Rmu_sc0001027.1_g000002
rosa_roxburghii	Rroxscaffold_2G00094120
rosa_rugosa	Rorug02G0444800
rosa_samantha	Rh2BG521400 Rh2CG495700 Rh2DG531500
rosa_wichuraiana	Rw2G041990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1649
Acc65I	GGTACC	1 cut(s)	554
AccB1I	GGYRCC	2 cut(s)	554, 1120
AccB7I	CCANNNNNTGG	2 cut(s)	899, 1988
AccI	GTMKAC	2 cut(s)	1801, 2191
AccII	CGCG	3 cut(s)	108, 190, 276
AciI	CCGC	4 cut(s)	74, 106, 183, 760
AclWI	GGATC	3 cut(s)	180, 758, 1780
AcsI	RAATTY	8 cut(s)	120, 494, 935, 959, 1219, 1634, 2024, 2148
AcuI	CTGAAG	3 cut(s)	1023, 2073, 2289
AcvI	CACGTG	1 cut(s)	529
AcyI	GRCGYC	2 cut(s)	53, 66
AfaI	GTAC	5 cut(s)	556, 973, 1441, 1735, 2337
AfeI	AGCGCT	1 cut(s)	16
AfiI	CCNNNNNNNGG	8 cut(s)	49, 899, 1130, 1436, 1811, 1988, 2248, 2379
AflIII	ACRYGT	3 cut(s)	526, 528, 1548
AjiI	CACGTC	2 cut(s)	1551, 2363
AjnI	CCWGG	2 cut(s)	1123, 2218
Alw21I	GWGCWC	1 cut(s)	1930
Alw26I	GTCTC	4 cut(s)	245, 358, 1913, 2287
AlwI	GGATC	3 cut(s)	180, 758, 1780
AlwNI	CAGNNNCTG	3 cut(s)	1463, 1922, 2411
Ama87I	CYCGRG	1 cut(s)	975
Aor51HI	AGCGCT	1 cut(s)	16
AoxI	GGCC	3 cut(s)	1678, 2215, 2432
ApoI	RAATTY	8 cut(s)	120, 494, 935, 959, 1219, 1634, 2024, 2148
ArsI	GACNNNNNNTTYG	2 cut(s)	1954, 1986
AseI	ATTAAT	1 cut(s)	1211
Asp700I	GAANNNNTTC	3 cut(s)	369, 2058, 2447
Asp718I	GGTACC	1 cut(s)	554
AspLEI	GCGC	2 cut(s)	17, 759
AspS9I	GGNCC	4 cut(s)	156, 1874, 2216, 2432
AsuC2I	CCSGG	1 cut(s)	2249
AsuHPI	GGTGA	5 cut(s)	1519, 1568, 1763, 1818, 1985
AsuII	TTCGAA	1 cut(s)	1048
AvaI	CYCGRG	1 cut(s)	975
AvaII	GGWCC	2 cut(s)	156, 1874
BanI	GGYRCC	2 cut(s)	554, 1120
BanII	GRGCYC	1 cut(s)	1930
BauI	CACGAG	2 cut(s)	1719, 2004
BbrPI	CACGTG	1 cut(s)	529
BbsI	GAAGAC	2 cut(s)	1211, 2205
Bbv12I	GWGCWC	1 cut(s)	1930
BccI	CCATC	7 cut(s)	329, 373, 1086, 1466, 1598, 1990, 2423
BceAI	ACGGC	3 cut(s)	116, 179, 2019
BciT130I	CCWGG	2 cut(s)	1125, 2220
BciVI	GTATCC	1 cut(s)	1939
BcnI	CCSGG	1 cut(s)	2249
BcoDI	GTCTC	4 cut(s)	245, 358, 1913, 2287
BfaI	CTAG	6 cut(s)	125, 1196, 1422, 1682, 1794, 2396
BfmI	CTRYAG	2 cut(s)	457, 920
BfoI	RGCGCY	2 cut(s)	18, 760
BfuI	GTATCC	1 cut(s)	1939
BlpI	GCTNAGC	1 cut(s)	240
Bme1390I	CCNGG	3 cut(s)	1125, 2220, 2249
Bme18I	GGWCC	2 cut(s)	156, 1874
BmeT110I	CYCGRG	1 cut(s)	975
BmgBI	CACGTC	2 cut(s)	1551, 2363
BmgT120I	GGNCC	4 cut(s)	156, 1874, 2216, 2432
BmiI	GGNNCC	3 cut(s)	157, 556, 1122
BmrFI	CCNGG	3 cut(s)	1125, 2220, 2249
BmsI	GCATC	5 cut(s)	103, 610, 641, 1322, 1871
BoxI	GACNNNNGTC	1 cut(s)	2261
BpiI	GAAGAC	2 cut(s)	1211, 2205
BpmI	CTGGAG	3 cut(s)	572, 1146, 1944
Bpu10I	CCTNAGC	2 cut(s)	165, 550
Bpu1102I	GCTNAGC	1 cut(s)	240
Bpu14I	TTCGAA	1 cut(s)	1048
BpuEI	CTTGAG	4 cut(s)	529, 1760, 1935, 2144
BpuMI	CCSGG	1 cut(s)	2249
Bsa29I	ATCGAT	1 cut(s)	1587
BsaAI	YACGTR	1 cut(s)	529
BsaBI	GATNNNNATC	1 cut(s)	2103
BsaHI	GRCGYC	2 cut(s)	53, 66
BsaJI	CCNNGG	6 cut(s)	159, 1144, 1668, 1727, 2218, 2247
BsaWI	WCCGGW	2 cut(s)	557, 2421
Bsc4I	CCNNNNNNNGG	8 cut(s)	49, 899, 1130, 1436, 1811, 1988, 2248, 2379
Bse1I	ACTGG	3 cut(s)	1468, 1840, 1927
Bse3DI	GCAATG	2 cut(s)	1719, 2111
Bse8I	GATNNNNATC	1 cut(s)	2103
BseBI	CCWGG	2 cut(s)	1125, 2220
BseCI	ATCGAT	1 cut(s)	1587
BseDI	CCNNGG	6 cut(s)	159, 1144, 1668, 1727, 2218, 2247
BseGI	GGATG	6 cut(s)	469, 949, 1258, 1477, 1636, 1977
BseJI	GATNNNNATC	1 cut(s)	2103
BseLI	CCNNNNNNNGG	8 cut(s)	49, 899, 1130, 1436, 1811, 1988, 2248, 2379
BseMI	GCAATG	2 cut(s)	1719, 2111
BseMII	CTCAG	4 cut(s)	231, 499, 591, 1557
BseNI	ACTGG	3 cut(s)	1468, 1840, 1927
BseRI	GAGGAG	1 cut(s)	1274
BseYI	CCCAGC	1 cut(s)	1130
BsgI	GTGCAG	5 cut(s)	312, 396, 1646, 2337, 2388
Bsh1236I	CGCG	3 cut(s)	108, 190, 276
BshFI	GGCC	3 cut(s)	1680, 2217, 2434
BshNI	GGYRCC	2 cut(s)	554, 1120
BshVI	ATCGAT	1 cut(s)	1587
BsiHKAI	GWGCWC	1 cut(s)	1930
BsiHKCI	CYCGRG	1 cut(s)	975
BsiSI	CCGG	5 cut(s)	63, 117, 558, 2249, 2422
BslFI	GGGAC	4 cut(s)	169, 916, 2062, 2249
BslI	CCNNNNNNNGG	8 cut(s)	49, 899, 1130, 1436, 1811, 1988, 2248, 2379
BsmAI	GTCTC	4 cut(s)	245, 358, 1913, 2287
BsmFI	GGGAC	4 cut(s)	169, 916, 2062, 2249
BsmI	GAATGC	1 cut(s)	828
BsnI	GGCC	3 cut(s)	1680, 2217, 2434
BsoBI	CYCGRG	1 cut(s)	975
Bsp119I	TTCGAA	1 cut(s)	1048
Bsp1286I	GDGCHC	1 cut(s)	1930
Bsp143I	GATC	7 cut(s)	172, 216, 750, 1509, 1785, 1830, 2179
Bsp1720I	GCTNAGC	1 cut(s)	240
Bsp19I	CCATGG	1 cut(s)	1727
Bsp68I	TCGCGA	1 cut(s)	276
BspACI	CCGC	4 cut(s)	74, 106, 183, 760
BspANI	GGCC	3 cut(s)	1680, 2217, 2434
BspCNI	CTCAG	4 cut(s)	232, 498, 592, 1558
BspDI	ATCGAT	1 cut(s)	1587
BspFNI	CGCG	3 cut(s)	108, 190, 276
BspLI	GGNNCC	3 cut(s)	157, 556, 1122
BspPI	GGATC	3 cut(s)	180, 758, 1780
BspQI	GCTCTTC	1 cut(s)	2453
BspT104I	TTCGAA	1 cut(s)	1048
BspT107I	GGYRCC	2 cut(s)	554, 1120
BsrDI	GCAATG	2 cut(s)	1719, 2111
BsrI	ACTGG	3 cut(s)	1468, 1840, 1927
BssECI	CCNNGG	6 cut(s)	159, 1144, 1668, 1727, 2218, 2247
BssMI	GATC	7 cut(s)	172, 216, 750, 1509, 1785, 1830, 2179
BssNAI	GTATAC	2 cut(s)	1802, 2192
BssNI	GRCGYC	2 cut(s)	53, 66
BssSI	CACGAG	2 cut(s)	1719, 2004
BssT1I	CCWWGG	3 cut(s)	159, 1668, 1727
Bst1107I	GTATAC	2 cut(s)	1802, 2192
Bst2BI	CACGAG	2 cut(s)	1719, 2004
Bst2UI	CCWGG	2 cut(s)	1125, 2220
Bst4CI	ACNGT	6 cut(s)	95, 417, 912, 1183, 2076, 2281
Bst6I	CTCTTC	2 cut(s)	1194, 2453
BstACI	GRCGYC	2 cut(s)	53, 66
BstAPI	GCANNNNNTGC	1 cut(s)	2018
BstBAI	YACGTR	1 cut(s)	529
BstBI	TTCGAA	1 cut(s)	1048
BstC8I	GCNNGC	6 cut(s)	192, 607, 1295, 1860, 1907, 2014
BstDEI	CTNAG	6 cut(s)	165, 240, 485, 550, 600, 1566
BstDSI	CCRYGG	2 cut(s)	1144, 1727
BstEII	GGTNACC	3 cut(s)	1663, 1751, 1991
BstF5I	GGATG	6 cut(s)	469, 949, 1258, 1477, 1636, 1977
BstFNI	CGCG	3 cut(s)	108, 190, 276
BstH2I	RGCGCY	2 cut(s)	18, 760
BstHHI	GCGC	2 cut(s)	17, 759
BstKTI	GATC	7 cut(s)	175, 219, 753, 1512, 1788, 1833, 2182
BstMAI	GTCTC	4 cut(s)	245, 358, 1913, 2287
BstMBI	GATC	7 cut(s)	172, 216, 750, 1509, 1785, 1830, 2179
BstMWI	GCNNNNNNNGC	5 cut(s)	79, 191, 629, 713, 2018
BstNI	CCWGG	2 cut(s)	1125, 2220
BstNSI	RCATGY	2 cut(s)	812, 1297
BstPAI	GACNNNNGTC	1 cut(s)	2261
BstPI	GGTNACC	3 cut(s)	1663, 1751, 1991
BstSCI	CCNGG	3 cut(s)	1123, 2218, 2247
BstSFI	CTRYAG	2 cut(s)	457, 920
BstUI	CGCG	3 cut(s)	108, 190, 276
BstV2I	GAAGAC	2 cut(s)	1211, 2205
BstX2I	RGATCY	1 cut(s)	172
BstYI	RGATCY	1 cut(s)	172
BstZ17I	GTATAC	2 cut(s)	1802, 2192
Bsu15I	ATCGAT	1 cut(s)	1587
BsuI	GTATCC	1 cut(s)	1939
BsuRI	GGCC	3 cut(s)	1680, 2217, 2434
BsuTUI	ATCGAT	1 cut(s)	1587
BtgI	CCRYGG	2 cut(s)	1144, 1727
BtgZI	GCGATG	1 cut(s)	1345
BtrI	CACGTC	2 cut(s)	1551, 2363
BtsCI	GGATG	6 cut(s)	469, 949, 1258, 1477, 1636, 1977
BtsI	GCAGTG	2 cut(s)	324, 1342
BtsIMutI	CAGTG	3 cut(s)	100, 324, 1342
BtuMI	TCGCGA	1 cut(s)	276
Cac8I	GCNNGC	6 cut(s)	192, 607, 1295, 1860, 1907, 2014
CaiI	CAGNNNCTG	3 cut(s)	1463, 1922, 2411
CfoI	GCGC	2 cut(s)	17, 759
Cfr13I	GGNCC	4 cut(s)	156, 1874, 2216, 2432
ClaI	ATCGAT	1 cut(s)	1587
CseI	GACGC	2 cut(s)	42, 55
Csp6I	GTAC	5 cut(s)	555, 972, 1440, 1734, 2336
CspCI	CAANNNNNGTGG	4 cut(s)	1126, 1161, 2290, 2325
CviAII	CATG	9 cut(s)	208, 747, 763, 804, 809, 1248, 1294, 1728, 2213
CviQI	GTAC	5 cut(s)	555, 972, 1440, 1734, 2336
DdeI	CTNAG	6 cut(s)	165, 240, 485, 550, 600, 1566
DpnI	GATC	7 cut(s)	174, 218, 752, 1511, 1787, 1832, 2181
DpnII	GATC	7 cut(s)	172, 216, 750, 1509, 1785, 1830, 2179
Eam1104I	CTCTTC	2 cut(s)	1194, 2453
EarI	CTCTTC	2 cut(s)	1194, 2453
Ecl136II	GAGCTC	1 cut(s)	1928
Eco130I	CCWWGG	3 cut(s)	159, 1668, 1727
Eco147I	AGGCCT	1 cut(s)	1680
Eco24I	GRGCYC	1 cut(s)	1930
Eco32I	GATATC	1 cut(s)	868
Eco47I	GGWCC	2 cut(s)	156, 1874
Eco47III	AGCGCT	1 cut(s)	16
Eco53kI	GAGCTC	1 cut(s)	1928
Eco57I	CTGAAG	3 cut(s)	1023, 2073, 2289
Eco72I	CACGTG	1 cut(s)	529
Eco88I	CYCGRG	1 cut(s)	975
Eco91I	GGTNACC	3 cut(s)	1663, 1751, 1991
EcoICRI	GAGCTC	1 cut(s)	1928
EcoO65I	GGTNACC	3 cut(s)	1663, 1751, 1991
EcoRI	GAATTC	2 cut(s)	120, 2148
EcoRII	CCWGG	2 cut(s)	1123, 2218
EcoRV	GATATC	1 cut(s)	868
EcoT14I	CCWWGG	3 cut(s)	159, 1668, 1727
EcoT22I	ATGCAT	2 cut(s)	625, 748
EcoT38I	GRGCYC	1 cut(s)	1930
ErhI	CCWWGG	3 cut(s)	159, 1668, 1727
FaeI	CATG	9 cut(s)	211, 750, 766, 807, 812, 1251, 1297, 1731, 2216
FalI	AAGNNNNNCTT	6 cut(s)	278, 310, 855, 887, 1307, 1339
FaqI	GGGAC	4 cut(s)	169, 916, 2062, 2249
FatI	CATG	9 cut(s)	207, 746, 762, 803, 808, 1247, 1293, 1727, 2212
FauNDI	CATATG	1 cut(s)	1402
FblI	GTMKAC	2 cut(s)	1801, 2191
FokI	GGATG	6 cut(s)	476, 956, 1265, 1484, 1643, 1964
FriOI	GRGCYC	1 cut(s)	1930
FspBI	CTAG	6 cut(s)	125, 1196, 1422, 1682, 1794, 2396
GlaI	GCGC	2 cut(s)	16, 758
GsaI	CCCAGC	1 cut(s)	1134
GsuI	CTGGAG	3 cut(s)	572, 1146, 1944
HaeII	RGCGCY	2 cut(s)	18, 760
HaeIII	GGCC	3 cut(s)	1680, 2217, 2434
HapII	CCGG	5 cut(s)	63, 117, 558, 2249, 2422
HgaI	GACGC	2 cut(s)	42, 55
HhaI	GCGC	2 cut(s)	17, 759
Hin1I	GRCGYC	2 cut(s)	53, 66
Hin1II	CATG	9 cut(s)	211, 750, 766, 807, 812, 1251, 1297, 1731, 2216
Hin6I	GCGC	2 cut(s)	15, 757
HinP1I	GCGC	2 cut(s)	15, 757
HincII	GTYRAC	1 cut(s)	140
HindII	GTYRAC	1 cut(s)	140
HindIII	AAGCTT	2 cut(s)	547, 1321
HpaII	CCGG	5 cut(s)	63, 117, 558, 2249, 2422
HphI	GGTGA	5 cut(s)	1519, 1568, 1763, 1818, 1985
Hpy166II	GTNNAC	8 cut(s)	140, 430, 1186, 1559, 1736, 1802, 1877, 2192
Hpy8I	GTNNAC	8 cut(s)	140, 430, 1186, 1559, 1736, 1802, 1877, 2192
Hpy99I	CGWCG	3 cut(s)	58, 71, 741
HpyAV	CCTTC	6 cut(s)	193, 229, 695, 807, 1829, 2244
HpyCH4III	ACNGT	6 cut(s)	95, 417, 912, 1183, 2076, 2281
HpyCH4IV	ACGT	4 cut(s)	528, 1550, 1879, 2362
HpyF10VI	GCNNNNNNNGC	5 cut(s)	79, 191, 629, 713, 2018
HpyF3I	CTNAG	6 cut(s)	165, 240, 485, 550, 600, 1566
HpySE526I	ACGT	4 cut(s)	528, 1550, 1879, 2362
Hsp92I	GRCGYC	2 cut(s)	53, 66
Hsp92II	CATG	9 cut(s)	211, 750, 766, 807, 812, 1251, 1297, 1731, 2216
HspAI	GCGC	2 cut(s)	15, 757
KpnI	GGTACC	1 cut(s)	558
Kzo9I	GATC	7 cut(s)	172, 216, 750, 1509, 1785, 1830, 2179
LguI	GCTCTTC	1 cut(s)	2453
LmnI	GCTCC	4 cut(s)	673, 704, 1127, 1925
LweI	GCATC	5 cut(s)	103, 610, 641, 1322, 1871
MaeI	CTAG	6 cut(s)	125, 1196, 1422, 1682, 1794, 2396
MaeII	ACGT	4 cut(s)	528, 1550, 1879, 2362
MaeIII	GTNAC	5 cut(s)	522, 578, 1663, 1751, 1991
MalI	GATC	7 cut(s)	174, 218, 752, 1511, 1787, 1832, 2181
MboI	GATC	7 cut(s)	172, 216, 750, 1509, 1785, 1830, 2179
MfeI	CAATTG	2 cut(s)	1595, 2082
MflI	RGATCY	1 cut(s)	172
MhlI	GDGCHC	1 cut(s)	1930
MlyI	GAGTC	1 cut(s)	816
MmeI	TCCRAC	5 cut(s)	270, 399, 702, 1599, 2290
Mph1103I	ATGCAT	2 cut(s)	625, 748
MroXI	GAANNNNTTC	3 cut(s)	369, 2058, 2447
MslI	CAYNNNNRTG	1 cut(s)	1781
MspA1I	CMGCKG	3 cut(s)	410, 1532, 2351
MspI	CCGG	5 cut(s)	63, 117, 558, 2249, 2422
MspR9I	CCNGG	3 cut(s)	1125, 2220, 2249
MunI	CAATTG	2 cut(s)	1595, 2082
Mva1269I	GAATGC	1 cut(s)	828
MvaI	CCWGG	2 cut(s)	1125, 2220
MvnI	CGCG	3 cut(s)	108, 190, 276
MwoI	GCNNNNNNNGC	5 cut(s)	79, 191, 629, 713, 2018
NciI	CCSGG	1 cut(s)	2249
NcoI	CCATGG	1 cut(s)	1727
NdeI	CATATG	1 cut(s)	1402
NdeII	GATC	7 cut(s)	172, 216, 750, 1509, 1785, 1830, 2179
NlaIII	CATG	9 cut(s)	211, 750, 766, 807, 812, 1251, 1297, 1731, 2216
NlaIV	GGNNCC	3 cut(s)	157, 556, 1122
NmuCI	GTSAC	3 cut(s)	522, 1751, 1991
NruI	TCGCGA	1 cut(s)	276
NsiI	ATGCAT	2 cut(s)	625, 748
NspI	RCATGY	2 cut(s)	812, 1297
NspV	TTCGAA	1 cut(s)	1048
PaeI	GCATGC	1 cut(s)	1297
PaeR7I	CTCGAG	1 cut(s)	975
PceI	AGGCCT	1 cut(s)	1680
PciSI	GCTCTTC	1 cut(s)	2453
PcsI	WCGNNNNNNNCGW	1 cut(s)	534
PctI	GAATGC	1 cut(s)	828
PdmI	GAANNNNTTC	3 cut(s)	369, 2058, 2447
PfeI	GAWTC	9 cut(s)	88, 100, 566, 1149, 1589, 2087, 2223, 2313, 2391
PflMI	CCANNNNNTGG	2 cut(s)	899, 1988
PleI	GAGTC	1 cut(s)	816
PmaCI	CACGTG	1 cut(s)	529
PmlI	CACGTG	1 cut(s)	529
PpsI	GAGTC	1 cut(s)	816
Ppu21I	YACGTR	1 cut(s)	529
PshAI	GACNNNNGTC	1 cut(s)	2261
PshBI	ATTAAT	1 cut(s)	1211
PsiI	TTATAA	1 cut(s)	1649
Psp124BI	GAGCTC	1 cut(s)	1930
Psp6I	CCWGG	2 cut(s)	1123, 2218
PspCI	CACGTG	1 cut(s)	529
PspEI	GGTNACC	3 cut(s)	1663, 1751, 1991
PspFI	CCCAGC	1 cut(s)	1130
PspGI	CCWGG	2 cut(s)	1123, 2218
PspN4I	GGNNCC	3 cut(s)	157, 556, 1122
PspPI	GGNCC	4 cut(s)	156, 1874, 2216, 2432
PspXI	VCTCGAGB	1 cut(s)	975
PstNI	CAGNNNCTG	3 cut(s)	1463, 1922, 2411
PsuI	RGATCY	1 cut(s)	172
PvuII	CAGCTG	3 cut(s)	410, 1532, 2351
RruI	TCGCGA	1 cut(s)	276
RsaI	GTAC	5 cut(s)	556, 973, 1441, 1735, 2337
RsaNI	GTAC	5 cut(s)	555, 972, 1440, 1734, 2336
RseI	CAYNNNNRTG	1 cut(s)	1781
SacI	GAGCTC	1 cut(s)	1930
SapI	GCTCTTC	1 cut(s)	2453
Sau3AI	GATC	7 cut(s)	172, 216, 750, 1509, 1785, 1830, 2179
Sau96I	GGNCC	4 cut(s)	156, 1874, 2216, 2432
SchI	GAGTC	1 cut(s)	816
ScrFI	CCNGG	3 cut(s)	1125, 2220, 2249
SduI	GDGCHC	1 cut(s)	1930
SfaNI	GCATC	5 cut(s)	103, 610, 641, 1322, 1871
SfcI	CTRYAG	2 cut(s)	457, 920
Sfr274I	CTCGAG	1 cut(s)	975
SfuI	TTCGAA	1 cut(s)	1048
SinI	GGWCC	2 cut(s)	156, 1874
SlaI	CTCGAG	1 cut(s)	975
SmiMI	CAYNNNNRTG	1 cut(s)	1781
SmlI	CTYRAG	5 cut(s)	544, 975, 1739, 1950, 2159
SmoI	CTYRAG	5 cut(s)	544, 975, 1739, 1950, 2159
SphI	GCATGC	1 cut(s)	1297
SseBI	AGGCCT	1 cut(s)	1680
SsiI	CCGC	4 cut(s)	74, 106, 183, 760
SspI	AATATT	2 cut(s)	516, 1538
SspMI	CTAG	6 cut(s)	125, 1196, 1422, 1682, 1794, 2396
SstI	GAGCTC	1 cut(s)	1930
StuI	AGGCCT	1 cut(s)	1680
StyD4I	CCNGG	3 cut(s)	1123, 2218, 2247
StyI	CCWWGG	3 cut(s)	159, 1668, 1727
TaaI	ACNGT	6 cut(s)	95, 417, 912, 1183, 2076, 2281
TaiI	ACGT	4 cut(s)	531, 1553, 1882, 2365
TaqI	TCGA	6 cut(s)	21, 69, 820, 976, 1048, 1587
TatI	WGTACW	1 cut(s)	2335
TauI	GCSGC	4 cut(s)	76, 108, 185, 762
TfiI	GAWTC	9 cut(s)	88, 100, 566, 1149, 1589, 2087, 2223, 2313, 2391
TscAI	CASTG	3 cut(s)	100, 331, 1342
TseFI	GTSAC	3 cut(s)	522, 1751, 1991
Tsp45I	GTSAC	3 cut(s)	522, 1751, 1991
TspDTI	ATGAA	8 cut(s)	323, 789, 792, 960, 1074, 1515, 1647, 1716
TspGWI	ACGGA	1 cut(s)	1133
TspRI	CASTG	3 cut(s)	100, 331, 1342
Van91I	CCANNNNNTGG	2 cut(s)	899, 1988
VpaK11BI	GGWCC	2 cut(s)	156, 1874
VspI	ATTAAT	1 cut(s)	1211
XapI	RAATTY	8 cut(s)	120, 494, 935, 959, 1219, 1634, 2024, 2148
XceI	RCATGY	2 cut(s)	812, 1297
XhoI	CTCGAG	1 cut(s)	975
XmiI	GTMKAC	2 cut(s)	1801, 2191
XmnI	GAANNNNTTC	3 cut(s)	369, 2058, 2447
XspI	CTAG	6 cut(s)	125, 1196, 1422, 1682, 1794, 2396
Zsp2I	ATGCAT	2 cut(s)	625, 748

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.