Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0010294

Physical Location & Seq

Genomic Coordinates Reverse (-)

10365559 .. 10374859

Gene Structure 9301 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1135000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2742 bp

ATGGCCCGCTCCAAAACAGTCGGGTCAGAGTCGTTGCTCCTCCGAAACCCGGTTCACTATCTCGTCGATCTTAGATCGACCCATCTTCCAGCGTCATTGCTGAATCGAAAGCAAAGCTTCAACTCTCAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGATACAGAGAGAGAGAAGAGAGAGAATCCACTCTCCAAATTCTCAGATCTCCTTCTTCCAACTGTTTCTCCCATCCATTACTTCAACGCAACGAGCTCAGAGCTTAATTTGGCAGCCATGGACAAGTCAAGCGCTTTGGAATACATCAACCAGATGTTTCCCACAGAGGCGTCTTTATCTGGTGTGGAACCGCTAATGCAGAAGATACACAGTGAGATTCGGCGTGTCGATGCTGAAATTTTAGCGGCGGTTCGCCAGCAGAGCAATTCGGGGACGAAAGCGAAAGAAGATCTCGCCGCTGCTACGCATGCCGTGGAGGAACTCATGTATAAAATCCGCGAAATTAAAACGAAAGCTGAGCAAAGTGAGACAATGGTTCAAGAAATATGTCGCGACATTAAGAAGTTAGACTTTGCAAAGAAGCATATAACAACGACGATTACTGCTCTTCATCGCCTGACCATGCTCGTCTCTGCTGTTGAGCAGCTCCAAGTAATGGCTTCAAAACGACAATATAAAGAGGCAGCTGCACAGTTGGAGGCTGTGAACCAGTTATGTAGCCATTTTGAGGCTTACAGGGACATTCCAAAAATCACCGAGCTGAGGGAAAAATTTAAGAATATCAAACAAATTCTAAAGTCGCATGTGTTTTCTGATTTCTCAAGCCTAGGTACTGGAAAGGAGTCGGAGGAAACTAATTTACTTCAGCAGTTATCTGATGCTTGCTTGGTTGTTGATGCTTTAGAGCCATCTGTGAGGGAAGAATTAGTAAATAACTTTTGCAGCAGGGAACTTACTTCCTATGAGCAGATCTTTGAAGGAGCTGAACTAGCTAAGTTGGATAAAACTGAACGAAGATATGCATGGATAAAGCGTCGTATGAGAACAAATGAAGAAATCTGGAAAATCTTTCCTACTTCATGGCATGTTCCTTATCGGCTCTGTATTCAGTTCTGTAAAAAAACAAGAAAGCAGCTGGAAGATATACATAATAATCAGAAAGAAAAGCCTGATGTTGGGACATTATTGTTGGCACTACAGCGAACTCTTGAATTTGAGGATGAATTGGCAGAGAAATTTGGTGGTGGTACTCGAGGCAGGGAGGTTGGAAATGAGATTGAAGAAATTGGTAGAGGGGAAAACACTAGTCAAAGTGCTTCAGATATTAGAAAGAAGTACGAAAAAAAGCTTGCTGCACATCAGGAAAGTAGCACAGAGGAAAAGGATAAAGAGTTATCAGCACCTGCAGCTGGGTTCAACTTCCGTGGAATCATTTCATCTTGCTTTGAGCCTCACTTGAATGTTTACACAGAACTAGAAGAGAAGACACTGATGGAGAATTTGGAGAAACTTGTTCAGGAGGAAACATGGGACATTGAGGAGGGAAGTCAGAATAATGTTTTATCAAGTAGCATGCAGTTGTTTCTTATAATCAAGAGGAGCTTAAAGCGATGCAGTGCTTTGACAAAGAACCAGACATTATTTAATTTGTTCAAGGTTTTTCAAAGGATTCTCAAGGCATATGCTACAAAGCTTTTTGTAAGACTGCCTAAAGGTGGTACAGGGATTGTTGCAGCCGCAACTGGTATGGACGGACAGATAAAGACATCAGACAGAGATGAAAGGGTGATTTGTTACATTGTCAACTCAGCTGAATACTGCCATAAGACGTCTGGTGAGCTGGCTGAAAGTGTATCCAAAATAATTGATACTCAGTTTGCTGATGGGGTGGATATGTCGGAAGTGCAGGATGAATTTTCAGCCGTTATAACAAAAGCATTAGTAACCTTGGTGCATGGCTTAGAAACTAAGTTTGATGCTGAAATGGCTGCAATGACACGAGTTCCATGGGCTACGCTTGAAAGTGTTGGTGACCAGTCAGAGTATGTTAATGGCATAAATATGATCCTCGTTACTAGTATTCCTATCCTTGGAACACTTCTTTCACCGATTTACTTCCAATTTTTCTTGGACAAGCTTGCGTCATCTCTTGGTCCACGTTTCTTTACTAACATTTTCAAATGCAAGCAAATATCAGAAACTGGAGCTCAACAAATGCTACTGGATACTCAAGCTGTAAAGACAATTCTTTTGGAGATACCATCCCTTGGTCACCAAACATCACGTGCTGCTAGCTATTCGAAATTTGTAAGCCGGGAGATGAGTAAAGCTGAAGCACTTCTGAAGGTCATACTGTCGCCAATTGATTCTGTTGCGGATACATATCGTGCATTACTACCAGAGGGAACGCCAATGGAGTTTCAGCGAATTTTGGAGCTCAAGGGTCTTAAGAAAGCAGATCAGCAAAGTATACTAGATGACTTTAACAAGCACGGCCCGGGAATCACACAACCTTCTCTCCCACCACCGGCTGCGCCACCAATCCCACTCCCAACTGCCCCCGCAGCTGCTCTGATACCAAACCCTGCTTCAGCCGGACTTATTGCATCCAGAGACGATGTGCTAGCCAGAGCAGCTGCACTCGGGCGAGGTGCAGCCACAACAGGGTTCAAGAGGTTCCTGGCTTTAACCGAAGCTGCAAAAGATCGAAAAGACGGGCCTTTCAGGAAGCTCTTCAACGCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0000938 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005794 GO:0006810 GO:0006890 GO:0006950 GO:0006970 GO:0007009 GO:0008150 GO:0009266 GO:0009408 GO:0009628 GO:0009987 GO:0010256 GO:0010286 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016197 GO:0016482 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0032991 GO:0042147 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044433 GO:0044444 GO:0044446 GO:0044464 GO:0046907 GO:0048193 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0061024 GO:0071840 GO:0097708 GO:0098588 GO:0098791 GO:0099023

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Vps53_N

Protein Analysis

914

Amino Acids

102.45

Weight (kDa)

6.83

Isoelectric Point (pI)

42.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Vps53_N	PF04100	94 - 507	2.4e-142	Vps53, N-terminal
VPS53_C	PF16854	737 - 815	6.8e-14	Vacuolar protein sorting-associated protein 53 C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0010294)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G50500 AT1G50500 AT1G50500 AT1G50500 AT1G50500
fragaria_vesca	FvH4_6g40130 FvH4_6g40130
malus_domestica	MD09G1135000.v1.1 MD17G1124000.v1.1
prunus_persica	Prupe.3G191700_v2.0.a1
pyrus_communis	pycom09g05610 pycom17g11540
rosa_chinensis	RchiOBHm_Chr2g0154761
rosa_laevigata	RLG00000020801
rosa_multiflora	Rmu_sc0001027.1_g000002
rosa_roxburghii	Rroxscaffold_2G00094120
rosa_rugosa	Rorug02G0444800
rosa_samantha	Rh2BG521400 Rh2CG495700 Rh2DG531500
rosa_wichuraiana	Rw2G041990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1586, 1925
AarI	CACCTGC	1 cut(s)	1408
AatII	GACGTC	1 cut(s)	1829
Acc36I	ACCTGC	1 cut(s)	1408
AccB7I	CCANNNNNTGG	2 cut(s)	652, 2264
AccBSI	CCGCTC	1 cut(s)	9
AccI	GTMKAC	1 cut(s)	2467
AccII	CGCG	2 cut(s)	495, 549
AciI	CCGC	9 cut(s)	7, 347, 401, 404, 453, 493, 1734, 2372, 2559
AclWI	GGATC	1 cut(s)	2056
AcuI	CTGAAG	5 cut(s)	845, 1299, 2349, 2360, 2571
AcvI	CACGTG	1 cut(s)	2282
AcyI	GRCGYC	2 cut(s)	326, 1826
AfaI	GTAC	4 cut(s)	829, 1246, 1334, 1717
AfeI	AGCGCT	1 cut(s)	289
AflII	CTTAAG	1 cut(s)	2444
AhlI	ACTAGT	2 cut(s)	1301, 2072
AjnI	CCWGG	1 cut(s)	2676
AjuI	GAANNNNNNNTTGG	2 cut(s)	2569, 2601
Alw21I	GWGCWC	3 cut(s)	254, 2206, 2436
Alw26I	GTCTC	3 cut(s)	518, 631, 2604
AlwI	GGATC	1 cut(s)	2056
AlwNI	CAGNNNCTG	2 cut(s)	1400, 2198
Ama87I	CYCGRG	3 cut(s)	1248, 2494, 2639
Aor51HI	AGCGCT	1 cut(s)	289
AoxI	GGCC	3 cut(s)	3, 2491, 2714
ArsI	GACNNNNNNTTYG	2 cut(s)	2230, 2262
Asp700I	GAANNNNTTC	3 cut(s)	1429, 2334, 2729
AspA2I	CCTAGG	1 cut(s)	823
AspLEI	GCGC	2 cut(s)	290, 2533
AspS9I	GGNCC	4 cut(s)	4, 2150, 2492, 2714
AsuC2I	CCSGG	4 cut(s)	50, 2312, 2495, 2496
AsuHPI	GGTGA	6 cut(s)	742, 1795, 1844, 2039, 2094, 2261
AsuII	TTCGAA	1 cut(s)	2297
AsuNHI	GCTAGC	2 cut(s)	2288, 2620
AvaI	CYCGRG	3 cut(s)	1248, 2494, 2639
AvaII	GGWCC	1 cut(s)	2150
AvrII	CCTAGG	1 cut(s)	823
BanII	GRGCYC	3 cut(s)	254, 2206, 2436
BauI	CACGAG	1 cut(s)	1995
BbrPI	CACGTG	1 cut(s)	2282
BbsI	GAAGAC	1 cut(s)	1487
Bbv12I	GWGCWC	3 cut(s)	254, 2206, 2436
BbvCI	CCTCAGC	1 cut(s)	758
BccI	CCATC	6 cut(s)	90, 236, 913, 1483, 1874, 2266
BceAI	ACGGC	3 cut(s)	452, 1904, 2506
BciT130I	CCWGG	1 cut(s)	2678
BciVI	GTATCC	3 cut(s)	1861, 2215, 2368
BcnI	CCSGG	4 cut(s)	50, 2312, 2495, 2496
BcoDI	GTCTC	3 cut(s)	518, 631, 2604
BcuI	ACTAGT	2 cut(s)	1301, 2072
BfaI	CTAG	8 cut(s)	824, 986, 1302, 1472, 2073, 2289, 2471, 2621
BfmI	CTRYAG	2 cut(s)	1193, 1401
BfoI	RGCGCY	1 cut(s)	291
BfrI	CTTAAG	1 cut(s)	2444
BfuAI	ACCTGC	1 cut(s)	1408
BfuI	GTATCC	3 cut(s)	1861, 2215, 2368
BglII	AGATCT	3 cut(s)	202, 445, 966
BlnI	CCTAGG	1 cut(s)	823
BlpI	GCTNAGC	1 cut(s)	513
Bme1390I	CCNGG	5 cut(s)	50, 2312, 2495, 2496, 2678
Bme18I	GGWCC	1 cut(s)	2150
BmeT110I	CYCGRG	3 cut(s)	1248, 2494, 2639
BmgT120I	GGNCC	4 cut(s)	4, 2150, 2492, 2714
BmiI	GGNNCC	2 cut(s)	345, 2675
BmrFI	CCNGG	5 cut(s)	50, 2312, 2495, 2496, 2678
BmsI	GCATC	6 cut(s)	376, 865, 883, 1598, 1963, 2612
BmtI	GCTAGC	2 cut(s)	2292, 2624
BpiI	GAAGAC	1 cut(s)	1487
BpmI	CTGGAG	1 cut(s)	2220
Bpu10I	CCTNAGC	1 cut(s)	758
Bpu1102I	GCTNAGC	1 cut(s)	513
Bpu14I	TTCGAA	1 cut(s)	2297
BpuEI	CTTGAG	4 cut(s)	802, 1655, 2211, 2420
BpuMI	CCSGG	4 cut(s)	50, 2312, 2495, 2496
BsaAI	YACGTR	1 cut(s)	2282
BsaBI	GATNNNNATC	1 cut(s)	2379
BsaHI	GRCGYC	2 cut(s)	326, 1826
BsaJI	CCNNGG	9 cut(s)	273, 468, 823, 1420, 1944, 2003, 2086, 2263, 2494
BsaXI	ACNNNNNCTCC	4 cut(s)	208, 238, 2499, 2529
Bse118I	RCCGGY	1 cut(s)	2524
Bse1I	ACTGG	6 cut(s)	706, 835, 1744, 2032, 2203, 2223
Bse3DI	GCAATG	2 cut(s)	95, 1995
Bse8I	GATNNNNATC	1 cut(s)	2379
BseBI	CCWGG	1 cut(s)	2678
BseDI	CCNNGG	9 cut(s)	273, 468, 823, 1420, 1944, 2003, 2086, 2263, 2494
BseGI	GGATG	5 cut(s)	228, 1222, 1912, 2258, 2603
BseJI	GATNNNNATC	1 cut(s)	2379
BseMI	GCAATG	2 cut(s)	95, 1995
BseMII	CTCAG	7 cut(s)	140, 213, 267, 504, 749, 1818, 1883
BseNI	ACTGG	6 cut(s)	706, 835, 1744, 2032, 2203, 2223
BseRI	GAGGAG	3 cut(s)	29, 1550, 1609
BseYI	CCCAGC	1 cut(s)	1406
BsgI	GTGCAG	5 cut(s)	669, 1335, 1922, 2619, 2670
Bsh1236I	CGCG	2 cut(s)	495, 549
BshFI	GGCC	3 cut(s)	5, 2493, 2716
BsiHKAI	GWGCWC	3 cut(s)	254, 2206, 2436
BsiHKCI	CYCGRG	3 cut(s)	1248, 2494, 2639
BsiSI	CCGG	5 cut(s)	50, 2311, 2495, 2525, 2592
BslFI	GGGAC	4 cut(s)	442, 749, 1189, 1541
BsmAI	GTCTC	3 cut(s)	518, 631, 2604
BsmBI	CGTCTC	2 cut(s)	631, 2604
BsmFI	GGGAC	4 cut(s)	442, 749, 1189, 1541
BsnI	GGCC	3 cut(s)	5, 2493, 2716
BsoBI	CYCGRG	3 cut(s)	1248, 2494, 2639
Bsp119I	TTCGAA	1 cut(s)	2297
Bsp1286I	GDGCHC	3 cut(s)	254, 2206, 2436
Bsp143I	GATC	8 cut(s)	67, 74, 202, 445, 966, 2061, 2455, 2701
Bsp1720I	GCTNAGC	1 cut(s)	513
Bsp19I	CCATGG	2 cut(s)	273, 2003
Bsp68I	TCGCGA	1 cut(s)	549
BspACI	CCGC	9 cut(s)	7, 347, 401, 404, 453, 493, 1734, 2372, 2559
BspANI	GGCC	3 cut(s)	5, 2493, 2716
BspCNI	CTCAG	7 cut(s)	139, 212, 266, 505, 750, 1817, 1882
BspFNI	CGCG	2 cut(s)	495, 549
BspLI	GGNNCC	2 cut(s)	345, 2675
BspMAI	CTGCAG	1 cut(s)	1405
BspMI	ACCTGC	1 cut(s)	1408
BspOI	GCTAGC	2 cut(s)	2292, 2624
BspPI	GGATC	1 cut(s)	2056
BspQI	GCTCTTC	2 cut(s)	609, 2735
BspT104I	TTCGAA	1 cut(s)	2297
BspTI	CTTAAG	1 cut(s)	2444
BsrBI	CCGCTC	1 cut(s)	9
BsrDI	GCAATG	2 cut(s)	95, 1995
BsrFI	RCCGGY	1 cut(s)	2524
BsrI	ACTGG	6 cut(s)	706, 835, 1744, 2032, 2203, 2223
BssAI	RCCGGY	1 cut(s)	2524
BssECI	CCNNGG	9 cut(s)	273, 468, 823, 1420, 1944, 2003, 2086, 2263, 2494
BssMI	GATC	8 cut(s)	67, 74, 202, 445, 966, 2061, 2455, 2701
BssNAI	GTATAC	1 cut(s)	2468
BssNI	GRCGYC	2 cut(s)	326, 1826
BssSI	CACGAG	1 cut(s)	1995
BssT1I	CCWWGG	6 cut(s)	273, 823, 1944, 2003, 2086, 2263
Bst1107I	GTATAC	1 cut(s)	2468
Bst2BI	CACGAG	1 cut(s)	1995
Bst2UI	CCWGG	1 cut(s)	2678
Bst4CI	ACNGT	5 cut(s)	19, 220, 368, 690, 2352
Bst6I	CTCTTC	4 cut(s)	167, 609, 1470, 2735
BstACI	GRCGYC	2 cut(s)	326, 1826
BstAFI	CTTAAG	1 cut(s)	2444
BstBAI	YACGTR	1 cut(s)	2282
BstBI	TTCGAA	1 cut(s)	2297
BstDSI	CCRYGG	4 cut(s)	273, 468, 1420, 2003
BstEII	GGTNACC	2 cut(s)	2027, 2267
BstF5I	GGATG	5 cut(s)	228, 1222, 1912, 2258, 2603
BstFNI	CGCG	2 cut(s)	495, 549
BstH2I	RGCGCY	1 cut(s)	291
BstHHI	GCGC	2 cut(s)	290, 2533
BstKTI	GATC	8 cut(s)	70, 77, 205, 448, 969, 2064, 2458, 2704
BstMAI	GTCTC	3 cut(s)	518, 631, 2604
BstMBI	GATC	8 cut(s)	67, 74, 202, 445, 966, 2061, 2455, 2701
BstMWI	GCNNNNNNNGC	7 cut(s)	417, 464, 986, 1403, 1982, 2561, 2630
BstNI	CCWGG	1 cut(s)	2678
BstNSI	RCATGY	4 cut(s)	467, 803, 1085, 1573
BstPI	GGTNACC	2 cut(s)	2027, 2267
BstSCI	CCNGG	5 cut(s)	48, 2310, 2493, 2494, 2676
BstSFI	CTRYAG	2 cut(s)	1193, 1401
BstUI	CGCG	2 cut(s)	495, 549
BstV2I	GAAGAC	1 cut(s)	1487
BstX2I	RGATCY	3 cut(s)	202, 445, 966
BstYI	RGATCY	3 cut(s)	202, 445, 966
BstZ17I	GTATAC	1 cut(s)	2468
BsuI	GTATCC	3 cut(s)	1861, 2215, 2368
BsuRI	GGCC	3 cut(s)	5, 2493, 2716
BtgI	CCRYGG	4 cut(s)	273, 468, 1420, 2003
BtgZI	GCGATG	2 cut(s)	593, 1621
BtsCI	GGATG	5 cut(s)	228, 1222, 1912, 2258, 2603
BtsI	GCAGTG	1 cut(s)	1618
BtsIMutI	CAGTG	3 cut(s)	373, 1484, 1618
BtuMI	TCGCGA	1 cut(s)	549
BveI	ACCTGC	1 cut(s)	1408
CaiI	CAGNNNCTG	2 cut(s)	1400, 2198
CfoI	GCGC	2 cut(s)	290, 2533
Cfr10I	RCCGGY	1 cut(s)	2524
Cfr13I	GGNCC	4 cut(s)	4, 2150, 2492, 2714
Cfr9I	CCCGGG	1 cut(s)	2494
CseI	GACGC	4 cut(s)	81, 315, 1019, 2127
Csp6I	GTAC	4 cut(s)	828, 1245, 1333, 1716
CspCI	CAANNNNNGTGG	2 cut(s)	1402, 1437
CviQI	GTAC	4 cut(s)	828, 1245, 1333, 1716
DpnI	GATC	8 cut(s)	69, 76, 204, 447, 968, 2063, 2457, 2703
DpnII	GATC	8 cut(s)	67, 74, 202, 445, 966, 2061, 2455, 2701
Eam1104I	CTCTTC	4 cut(s)	167, 609, 1470, 2735
EarI	CTCTTC	4 cut(s)	167, 609, 1470, 2735
Ecl136II	GAGCTC	3 cut(s)	252, 2204, 2434
Eco130I	CCWWGG	6 cut(s)	273, 823, 1944, 2003, 2086, 2263
Eco24I	GRGCYC	3 cut(s)	254, 2206, 2436
Eco47I	GGWCC	1 cut(s)	2150
Eco47III	AGCGCT	1 cut(s)	289
Eco53kI	GAGCTC	3 cut(s)	252, 2204, 2434
Eco57I	CTGAAG	5 cut(s)	845, 1299, 2349, 2360, 2571
Eco72I	CACGTG	1 cut(s)	2282
Eco88I	CYCGRG	3 cut(s)	1248, 2494, 2639
Eco91I	GGTNACC	2 cut(s)	2027, 2267
EcoICRI	GAGCTC	3 cut(s)	252, 2204, 2434
EcoO65I	GGTNACC	2 cut(s)	2027, 2267
EcoRII	CCWGG	1 cut(s)	2676
EcoT14I	CCWWGG	6 cut(s)	273, 823, 1944, 2003, 2086, 2263
EcoT22I	ATGCAT	1 cut(s)	1021
EcoT38I	GRGCYC	3 cut(s)	254, 2206, 2436
ErhI	CCWWGG	6 cut(s)	273, 823, 1944, 2003, 2086, 2263
Esp3I	CGTCTC	2 cut(s)	631, 2604
FalI	AAGNNNNNCTT	6 cut(s)	101, 133, 551, 583, 1583, 1615
FaqI	GGGAC	4 cut(s)	442, 749, 1189, 1541
FauI	CCCGC	2 cut(s)	14, 2566
FauNDI	CATATG	1 cut(s)	1678
FblI	GTMKAC	1 cut(s)	2467
FokI	GGATG	5 cut(s)	215, 1229, 1919, 2245, 2590
FriOI	GRGCYC	3 cut(s)	254, 2206, 2436
FspBI	CTAG	8 cut(s)	824, 986, 1302, 1472, 2073, 2289, 2471, 2621
GlaI	GCGC	2 cut(s)	289, 2532
GsaI	CCCAGC	1 cut(s)	1410
GsuI	CTGGAG	1 cut(s)	2220
HaeII	RGCGCY	1 cut(s)	291
HaeIII	GGCC	3 cut(s)	5, 2493, 2716
HapII	CCGG	5 cut(s)	50, 2311, 2495, 2525, 2592
HgaI	GACGC	4 cut(s)	81, 315, 1019, 2127
HhaI	GCGC	2 cut(s)	290, 2533
Hin1I	GRCGYC	2 cut(s)	326, 1826
Hin6I	GCGC	2 cut(s)	288, 2531
HinP1I	GCGC	2 cut(s)	288, 2531
HincII	GTYRAC	1 cut(s)	1801
HindII	GTYRAC	1 cut(s)	1801
HindIII	AAGCTT	4 cut(s)	115, 1343, 1688, 2132
HinfI	GANTC	9 cut(s)	29, 103, 181, 373, 839, 1425, 1666, 2363, 2499
HpaII	CCGG	5 cut(s)	50, 2311, 2495, 2525, 2592
HphI	GGTGA	6 cut(s)	742, 1795, 1844, 2039, 2094, 2261
Hpy166II	GTNNAC	6 cut(s)	55, 703, 1462, 1801, 2153, 2468
Hpy8I	GTNNAC	6 cut(s)	55, 703, 1462, 1801, 2153, 2468
Hpy99I	CGWCG	3 cut(s)	68, 595, 1035
HpyAV	CCTTC	4 cut(s)	218, 968, 2335, 2520
HpyCH4III	ACNGT	5 cut(s)	19, 220, 368, 690, 2352
HpyCH4IV	ACGT	3 cut(s)	1826, 2155, 2281
HpyF10VI	GCNNNNNNNGC	7 cut(s)	417, 464, 986, 1403, 1982, 2561, 2630
HpySE526I	ACGT	3 cut(s)	1826, 2155, 2281
Hsp92I	GRCGYC	2 cut(s)	326, 1826
HspAI	GCGC	2 cut(s)	288, 2531
Kzo9I	GATC	8 cut(s)	67, 74, 202, 445, 966, 2061, 2455, 2701
LguI	GCTCTTC	2 cut(s)	609, 2735
LmnI	GCTCC	7 cut(s)	14, 42, 648, 977, 1596, 2201, 2431
LweI	GCATC	6 cut(s)	376, 865, 883, 1598, 1963, 2612
MaeI	CTAG	8 cut(s)	824, 986, 1302, 1472, 2073, 2289, 2471, 2621
MaeII	ACGT	3 cut(s)	1826, 2155, 2281
MaeIII	GTNAC	5 cut(s)	1790, 1939, 2027, 2068, 2267
MalI	GATC	8 cut(s)	69, 76, 204, 447, 968, 2063, 2457, 2703
MbiI	CCGCTC	1 cut(s)	9
MboI	GATC	8 cut(s)	67, 74, 202, 445, 966, 2061, 2455, 2701
MfeI	CAATTG	1 cut(s)	2358
MflI	RGATCY	3 cut(s)	202, 445, 966
MhlI	GDGCHC	3 cut(s)	254, 2206, 2436
MlyI	GAGTC	2 cut(s)	38, 848
MmeI	TCCRAC	6 cut(s)	239, 672, 822, 975, 1243, 1875
Mph1103I	ATGCAT	1 cut(s)	1021
MroXI	GAANNNNTTC	3 cut(s)	1429, 2334, 2729
MslI	CAYNNNNRTG	2 cut(s)	1455, 2057
MspA1I	CMGCKG	7 cut(s)	455, 683, 1132, 1406, 1808, 2564, 2633
MspCI	CTTAAG	1 cut(s)	2444
MspI	CCGG	5 cut(s)	50, 2311, 2495, 2525, 2592
MspR9I	CCNGG	5 cut(s)	50, 2312, 2495, 2496, 2678
MunI	CAATTG	1 cut(s)	2358
MvaI	CCWGG	1 cut(s)	2678
MvnI	CGCG	2 cut(s)	495, 549
MwoI	GCNNNNNNNGC	7 cut(s)	417, 464, 986, 1403, 1982, 2561, 2630
NciI	CCSGG	4 cut(s)	50, 2312, 2495, 2496
NcoI	CCATGG	2 cut(s)	273, 2003
NdeI	CATATG	1 cut(s)	1678
NdeII	GATC	8 cut(s)	67, 74, 202, 445, 966, 2061, 2455, 2701
NheI	GCTAGC	2 cut(s)	2288, 2620
NlaIV	GGNNCC	2 cut(s)	345, 2675
NmuCI	GTSAC	2 cut(s)	2027, 2267
NruI	TCGCGA	1 cut(s)	549
NsiI	ATGCAT	1 cut(s)	1021
NspI	RCATGY	4 cut(s)	467, 803, 1085, 1573
NspV	TTCGAA	1 cut(s)	2297
PaeI	GCATGC	2 cut(s)	467, 1573
PaeR7I	CTCGAG	1 cut(s)	1248
PaqCI	CACCTGC	1 cut(s)	1408
PciSI	GCTCTTC	2 cut(s)	609, 2735
PdmI	GAANNNNTTC	3 cut(s)	1429, 2334, 2729
PfeI	GAWTC	7 cut(s)	103, 181, 373, 1425, 1666, 2363, 2499
PflMI	CCANNNNNTGG	2 cut(s)	652, 2264
PleI	GAGTC	2 cut(s)	37, 847
PmaCI	CACGTG	1 cut(s)	2282
PmlI	CACGTG	1 cut(s)	2282
PpsI	GAGTC	2 cut(s)	37, 847
Ppu21I	YACGTR	1 cut(s)	2282
PsiI	TTATAA	2 cut(s)	1586, 1925
Psp124BI	GAGCTC	3 cut(s)	254, 2206, 2436
Psp6I	CCWGG	1 cut(s)	2676
PspCI	CACGTG	1 cut(s)	2282
PspEI	GGTNACC	2 cut(s)	2027, 2267
PspFI	CCCAGC	1 cut(s)	1406
PspGI	CCWGG	1 cut(s)	2676
PspN4I	GGNNCC	2 cut(s)	345, 2675
PspPI	GGNCC	4 cut(s)	4, 2150, 2492, 2714
PspXI	VCTCGAGB	1 cut(s)	1248
PstI	CTGCAG	1 cut(s)	1405
PstNI	CAGNNNCTG	2 cut(s)	1400, 2198
PsuI	RGATCY	3 cut(s)	202, 445, 966
PvuII	CAGCTG	6 cut(s)	683, 1132, 1406, 1808, 2564, 2633
RruI	TCGCGA	1 cut(s)	549
RsaI	GTAC	4 cut(s)	829, 1246, 1334, 1717
RsaNI	GTAC	4 cut(s)	828, 1245, 1333, 1716
RseI	CAYNNNNRTG	2 cut(s)	1455, 2057
SacI	GAGCTC	3 cut(s)	254, 2206, 2436
SapI	GCTCTTC	2 cut(s)	609, 2735
Sau3AI	GATC	8 cut(s)	67, 74, 202, 445, 966, 2061, 2455, 2701
Sau96I	GGNCC	4 cut(s)	4, 2150, 2492, 2714
SchI	GAGTC	2 cut(s)	38, 848
ScrFI	CCNGG	5 cut(s)	50, 2312, 2495, 2496, 2678
SduI	GDGCHC	3 cut(s)	254, 2206, 2436
SfaNI	GCATC	6 cut(s)	376, 865, 883, 1598, 1963, 2612
SfcI	CTRYAG	2 cut(s)	1193, 1401
Sfr274I	CTCGAG	1 cut(s)	1248
SfuI	TTCGAA	1 cut(s)	2297
SinI	GGWCC	1 cut(s)	2150
SlaI	CTCGAG	1 cut(s)	1248
SmaI	CCCGGG	1 cut(s)	2496
SmiMI	CAYNNNNRTG	2 cut(s)	1455, 2057
SmlI	CTYRAG	6 cut(s)	817, 1248, 1670, 2226, 2435, 2444
SmoI	CTYRAG	6 cut(s)	817, 1248, 1670, 2226, 2435, 2444
SpeI	ACTAGT	2 cut(s)	1301, 2072
SphI	GCATGC	2 cut(s)	467, 1573
SsiI	CCGC	9 cut(s)	7, 347, 401, 404, 453, 493, 1734, 2372, 2559
SspMI	CTAG	8 cut(s)	824, 986, 1302, 1472, 2073, 2289, 2471, 2621
SstI	GAGCTC	3 cut(s)	254, 2206, 2436
StyD4I	CCNGG	5 cut(s)	48, 2310, 2493, 2494, 2676
StyI	CCWWGG	6 cut(s)	273, 823, 1944, 2003, 2086, 2263
TaaI	ACNGT	5 cut(s)	19, 220, 368, 690, 2352
TaiI	ACGT	3 cut(s)	1829, 2158, 2284
TaqI	TCGA	7 cut(s)	66, 77, 106, 384, 1249, 2297, 2704
TauI	GCSGC	3 cut(s)	404, 455, 1736
TfiI	GAWTC	7 cut(s)	103, 181, 373, 1425, 1666, 2363, 2499
TscAI	CASTG	3 cut(s)	373, 1491, 1618
TseFI	GTSAC	2 cut(s)	2027, 2267
Tsp45I	GTSAC	2 cut(s)	2027, 2267
TspDTI	ATGAA	7 cut(s)	596, 1062, 1065, 1233, 1422, 1791, 1923
TspGWI	ACGGA	2 cut(s)	1409, 1764
TspMI	CCCGGG	1 cut(s)	2494
TspRI	CASTG	3 cut(s)	373, 1491, 1618
Van91I	CCANNNNNTGG	2 cut(s)	652, 2264
Vha464I	CTTAAG	1 cut(s)	2444
VpaK11BI	GGWCC	1 cut(s)	2150
XceI	RCATGY	4 cut(s)	467, 803, 1085, 1573
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1826
XhoI	CTCGAG	1 cut(s)	1248
XmaI	CCCGGG	1 cut(s)	2494
XmaJI	CCTAGG	1 cut(s)	823
XmiI	GTMKAC	1 cut(s)	2467
XmnI	GAANNNNTTC	3 cut(s)	1429, 2334, 2729
XspI	CTAG	8 cut(s)	824, 986, 1302, 1472, 2073, 2289, 2471, 2621
ZraI	GACGTC	1 cut(s)	1827
Zsp2I	ATGCAT	1 cut(s)	1021

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.