AT1G50950

Disulfide-isomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0009964
Physical Location & Seq
Forward (+)
18880112 .. 18884326
4215 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G50950.2

Sequence Viewer

Length: 1455 bp
ATGGTTTCAACCTCCAAGATCAAGTCCGTTGATTTCTACAGGAAAATCCCAAGAGATTTGACTGAAGCATCTCTCTCTGGTGCTGGATTATCCATTGTAGCTGCCCTTGCTATGTTGTTTTTGTTTGGAATGGAGCTGAGTAGTTATTTGGCTATTAACACTAGCACATCTGTAATAGTTGACAAAAGCTCTGATGGGGACTTCTTAAACATTGATTTTAACATCAGCTTTCCTGCCCTCTCATGTGAATTCGCATCAGTTGACGTGAGTGACGTGTTTGGAACTCACAGGTTAAATATATCGAAAACGATTAGAAAAGTTCCAATTGATCCGCATTTAAGAGCCACTGCTGAGGAATTTCACTCCACCTCTGATTTACATCTCATCAACCATGGAGACGAAGATCATGGTGACAATAGTACTTATGCGGATATACCCTTAACTGGTGCTGCTTTTGAGAAGTTTACACATCATTTTCAAATTTTGGTTGTTAATTTTTATGCGCCATGGTGCTACTGGAGTAATCGCCTGAAACCATCTTGGGTGAAAGCATCTCAAATAACGAGAGAAAGATATAATCCTGGAACTGATGATCGTGTTCTTTTAGGAAGTGTTGATTGCACAGAAGAACCTACCCTATGTAAGAGCAATCATATACAAGGCTATCCATCTATTCGGATTTTCCGCAGAGGCAGTGGTCTTAGGGAGGACCATGGGAACCACGAACATGAATCTTACTATGGAGATCGGGACACAGATAGCTTAGTCAAGATGGTGGAAGAACTGCTCAAACCTATTAAAAAGGAGGATCATAAGTTGGCTTTGGATGGTAAATCTGATAATGCGGCTTCAACTTTTAAAAAGGCACCAGTTAGTGGAGGTTGTCGAATTGAAGGATATGTTCGCGCAAAGAAGGTTCCCGGCGAACTTGTAATCTCAGCTCATTCAGGAGCTCATTCATTTGATGCTTCTCAAATGAACATGTCTCATATTGTTACCCATCTAACATTTGGTACAATGGTTTCAGAAAGGTTGTGGACTGATATGAAACGATTACTGCCTTATCTTGGCCAAAGCTATGATAGGCTGAATGGAAAATCGTTCATCAATGAACGACAATTGGATGCTAATGTTACAATCGAACACTACTTGCAAATAATCAAAACAGAGGTCATTTCAAGAAGATCCGGCCAAGAACACTCATTGATTGAGGAATATGAATATACGGCTCATAGCAGCGTAGCTCGTAGTTACCATTATCCTGAAGCAAAGTTTCACTTTGAGCTCTCTCCAATGCAGGTCCTAATAAGCGAGAACCCAAAGTCATTCTCGCACTTCATCACAAATGTTTGCGCCATAATAGGAGGTGTTTTTACGGTCGCGGGAATACTAGATTCTATATTTCAAAACACAGTTAGAATGGTGAAAAAGATCGAACTCGGGAAAAACATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003756 GO:0003824 GO:0016853 GO:0016860 GO:0016864 GO:0140096
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
COPIIcoated_ERV ERGIC_N Thioredoxin

Protein Analysis

484

Amino Acids

54.36

Weight (kDa)

6.37

Isoelectric Point (pI)

38.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ERGIC_N PF13850 7 - 97 3.9e-23 Endoplasmic Reticulum-Golgi Intermediate Compartment (ERGIC)
Thioredoxin PF00085 147 - 248 6.2e-11 Thioredoxin
COPIIcoated_ERV PF07970 293 - 470 2.9e-25 Endoplasmic reticulum vesicle transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009964)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G50950 AT3G20560 AT4G27080
fragaria_vesca FvH4_3g21990 FvH4_3g21990 FvH4_3g21990
malus_domestica MD03G1209500.v1.1 MD11G1224300.v1.1
prunus_persica Prupe.4G205600_v2.0.a1
pyrus_communis pycom03g15830 pycom11g19790 pycom11g19800
rosa_chinensis RchiOBHm_Chr5g0037841
rosa_laevigata RLG00000033816
rosa_multiflora Rmu_sc0005416.1_g000015
rosa_roxburghii Rroxscaffold_1G00043080
rosa_rugosa Rorug05G0168000 Rorug05G0168100
rosa_samantha Rh5AG256900 Rh5DG269200
rosa_wichuraiana Rw5G023820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1288
AccB1I GGYRCC 1 cut(s) 865
AccB7I CCANNNNNTGG 1 cut(s) 875
AccII CGCG 2 cut(s) 906, 1382
AciI CCGC 5 cut(s) 332, 428, 685, 845, 1382
AclWI GGATC 3 cut(s) 323, 816, 1179
AcoI YGGCCR 2 cut(s) 1069, 1189
AcsI RAATTY 3 cut(s) 248, 356, 480
AcuI CTGAAG 2 cut(s) 84, 1284
AfaI GTAC 2 cut(s) 421, 1015
AfiI CCNNNNNNNGG 3 cut(s) 443, 875, 1067
AflIII ACRYGT 2 cut(s) 273, 981
AgsI TTSAA 6 cut(s) 9, 479, 852, 893, 1179, 1406
AjiI CACGTC 2 cut(s) 265, 274
AjnI CCWGG 1 cut(s) 580
Alw21I GWGCWC 2 cut(s) 955, 1287
Alw26I GTCTC 2 cut(s) 390, 990
AlwI GGATC 3 cut(s) 323, 816, 1179
Ama87I CYCGRG 1 cut(s) 1439
AoxI GGCC 2 cut(s) 1069, 1189
ApeKI GCWGC 3 cut(s) 101, 449, 1236
ApoI RAATTY 3 cut(s) 248, 356, 480
ArsI GACNNNNNNTTYG 2 cut(s) 1155, 1187
AspLEI GCGC 3 cut(s) 505, 908, 1355
AspS9I GGNCC 2 cut(s) 709, 1300
AsuC2I CCSGG 1 cut(s) 921
AsuHPI GGTGA 3 cut(s) 422, 556, 1435
AvaI CYCGRG 1 cut(s) 1439
AvaII GGWCC 2 cut(s) 709, 1300
BaeI ACNNNNGTAYC 2 cut(s) 1005, 1038
BalI TGGCCA 1 cut(s) 1071
BanI GGYRCC 1 cut(s) 865
BanII GRGCYC 2 cut(s) 955, 1287
Bbv12I GWGCWC 2 cut(s) 955, 1287
BbvCI CCTCAGC 1 cut(s) 351
BbvI GCAGC 3 cut(s) 88, 436, 1248
BccI CCATC 6 cut(s) 188, 544, 676, 766, 821, 1008
BceAI ACGGC 1 cut(s) 1242
BciT130I CCWGG 1 cut(s) 582
BcnI CCSGG 1 cut(s) 921
BcoDI GTCTC 2 cut(s) 390, 990
BfaI CTAG 2 cut(s) 162, 1391
BfmI CTRYAG 1 cut(s) 37
BfuAI ACCTGC 1 cut(s) 1288
BisI GCNGC 4 cut(s) 102, 450, 846, 1237
BlsI GCNGC 4 cut(s) 103, 451, 847, 1238
BmcAI AGTACT 1 cut(s) 421
Bme1390I CCNGG 2 cut(s) 582, 921
Bme18I GGWCC 2 cut(s) 709, 1300
BmeT110I CYCGRG 1 cut(s) 1439
BmgBI CACGTC 2 cut(s) 265, 274
BmgT120I GGNCC 2 cut(s) 709, 1300
BmiI GGNNCC 3 cut(s) 719, 867, 918
BmrFI CCNGG 2 cut(s) 582, 921
BmsI GCATC 5 cut(s) 77, 263, 560, 955, 1114
BpmI CTGGAG 1 cut(s) 538
Bpu10I CCTNAGC 1 cut(s) 351
BpuMI CCSGG 1 cut(s) 921
BsaBI GATNNNNATC 1 cut(s) 378
BsaJI CCNNGG 3 cut(s) 391, 506, 712
Bsc4I CCNNNNNNNGG 3 cut(s) 443, 875, 1067
Bse1I ACTGG 3 cut(s) 448, 521, 869
Bse8I GATNNNNATC 1 cut(s) 378
BseBI CCWGG 1 cut(s) 582
BseDI CCNNGG 3 cut(s) 391, 506, 712
BseGI GGATG 2 cut(s) 832, 1129
BseJI GATNNNNATC 1 cut(s) 378
BseLI CCNNNNNNNGG 3 cut(s) 443, 875, 1067
BseMII CTCAG 3 cut(s) 128, 342, 951
BseNI ACTGG 3 cut(s) 448, 521, 869
BseXI GCAGC 3 cut(s) 88, 436, 1248
Bsh1236I CGCG 2 cut(s) 906, 1382
Bsh1285I CGRYCG 1 cut(s) 1380
BshFI GGCC 2 cut(s) 1071, 1191
BshNI GGYRCC 1 cut(s) 865
BsiEI CGRYCG 1 cut(s) 1380
BsiHKAI GWGCWC 2 cut(s) 955, 1287
BsiHKCI CYCGRG 1 cut(s) 1439
BsiSI CCGG 2 cut(s) 921, 1188
BslFI GGGAC 2 cut(s) 212, 764
BslI CCNNNNNNNGG 3 cut(s) 443, 875, 1067
BsmAI GTCTC 2 cut(s) 390, 990
BsmBI CGTCTC 1 cut(s) 390
BsmFI GGGAC 2 cut(s) 212, 764
BsnI GGCC 2 cut(s) 1071, 1191
BsoBI CYCGRG 1 cut(s) 1439
Bsp1286I GDGCHC 2 cut(s) 955, 1287
Bsp143I GATC 8 cut(s) 18, 328, 403, 592, 745, 808, 1184, 1431
Bsp19I CCATGG 3 cut(s) 391, 506, 712
BspACI CCGC 5 cut(s) 332, 428, 685, 845, 1382
BspANI GGCC 2 cut(s) 1071, 1191
BspCNI CTCAG 3 cut(s) 129, 343, 950
BspFNI CGCG 2 cut(s) 906, 1382
BspLI GGNNCC 3 cut(s) 719, 867, 918
BspMI ACCTGC 1 cut(s) 1288
BspPI GGATC 3 cut(s) 323, 816, 1179
BspT107I GGYRCC 1 cut(s) 865
BsrI ACTGG 3 cut(s) 448, 521, 869
BssECI CCNNGG 3 cut(s) 391, 506, 712
BssMI GATC 8 cut(s) 18, 328, 403, 592, 745, 808, 1184, 1431
BssT1I CCWWGG 3 cut(s) 391, 506, 712
Bst2UI CCWGG 1 cut(s) 582
Bst4CI ACNGT 2 cut(s) 1378, 1414
BstDEI CTNAG 5 cut(s) 137, 351, 701, 763, 937
BstDSI CCRYGG 3 cut(s) 391, 506, 712
BstF5I GGATG 2 cut(s) 832, 1129
BstFNI CGCG 2 cut(s) 906, 1382
BstHHI GCGC 3 cut(s) 505, 908, 1355
BstKTI GATC 8 cut(s) 21, 331, 406, 595, 748, 811, 1187, 1434
BstMAI GTCTC 2 cut(s) 390, 990
BstMBI GATC 8 cut(s) 18, 328, 403, 592, 745, 808, 1184, 1431
BstMCI CGRYCG 1 cut(s) 1380
BstMWI GCNNNNNNNGC 1 cut(s) 107
BstNI CCWGG 1 cut(s) 582
BstNSI RCATGY 1 cut(s) 985
BstSCI CCNGG 2 cut(s) 580, 919
BstSFI CTRYAG 1 cut(s) 37
BstUI CGCG 2 cut(s) 906, 1382
BstV1I GCAGC 3 cut(s) 88, 436, 1248
BstX2I RGATCY 1 cut(s) 1184
BstYI RGATCY 1 cut(s) 1184
BsuRI GGCC 2 cut(s) 1071, 1191
BtgI CCRYGG 3 cut(s) 391, 506, 712
BtrI CACGTC 2 cut(s) 265, 274
BtsCI GGATG 2 cut(s) 832, 1129
BtsI GCAGTG 2 cut(s) 345, 700
BtsIMutI CAGTG 2 cut(s) 345, 700
BveI ACCTGC 1 cut(s) 1288
CfoI GCGC 3 cut(s) 505, 908, 1355
Cfr13I GGNCC 2 cut(s) 709, 1300
Csp6I GTAC 2 cut(s) 420, 1014
CviAII CATG 7 cut(s) 243, 392, 407, 507, 713, 728, 982
CviQI GTAC 2 cut(s) 420, 1014
DdeI CTNAG 5 cut(s) 137, 351, 701, 763, 937
DpnI GATC 8 cut(s) 20, 330, 405, 594, 747, 810, 1186, 1433
DpnII GATC 8 cut(s) 18, 328, 403, 592, 745, 808, 1184, 1431
DraI TTTAAA 1 cut(s) 859
EaeI YGGCCR 2 cut(s) 1069, 1189
Ecl136II GAGCTC 2 cut(s) 953, 1285
Eco130I CCWWGG 3 cut(s) 391, 506, 712
Eco24I GRGCYC 2 cut(s) 955, 1287
Eco47I GGWCC 2 cut(s) 709, 1300
Eco53kI GAGCTC 2 cut(s) 953, 1285
Eco57I CTGAAG 2 cut(s) 84, 1284
Eco88I CYCGRG 1 cut(s) 1439
EcoICRI GAGCTC 2 cut(s) 953, 1285
EcoO109I RGGNCCY 1 cut(s) 1300
EcoRI GAATTC 1 cut(s) 248
EcoRII CCWGG 1 cut(s) 580
EcoT14I CCWWGG 3 cut(s) 391, 506, 712
EcoT38I GRGCYC 2 cut(s) 955, 1287
ErhI CCWWGG 3 cut(s) 391, 506, 712
Esp3I CGTCTC 1 cut(s) 390
FaeI CATG 7 cut(s) 246, 395, 410, 510, 716, 731, 985
FalI AAGNNNNNCTT 2 cut(s) 1262, 1294
FaqI GGGAC 2 cut(s) 212, 764
FatI CATG 7 cut(s) 242, 391, 406, 506, 712, 727, 981
FauI CCCGC 1 cut(s) 1375
Fnu4HI GCNGC 4 cut(s) 102, 450, 846, 1237
FokI GGATG 2 cut(s) 839, 1136
FriOI GRGCYC 2 cut(s) 955, 1287
Fsp4HI GCNGC 4 cut(s) 102, 450, 846, 1237
FspBI CTAG 2 cut(s) 162, 1391
GlaI GCGC 3 cut(s) 504, 907, 1354
GluI GCNGC 4 cut(s) 102, 450, 846, 1237
GsuI CTGGAG 1 cut(s) 538
HaeIII GGCC 2 cut(s) 1071, 1191
HapII CCGG 2 cut(s) 921, 1188
HhaI GCGC 3 cut(s) 505, 908, 1355
Hin1II CATG 7 cut(s) 246, 395, 410, 510, 716, 731, 985
Hin6I GCGC 3 cut(s) 503, 906, 1353
HinP1I GCGC 3 cut(s) 503, 906, 1353
HincII GTYRAC 2 cut(s) 181, 262
HindII GTYRAC 2 cut(s) 181, 262
HinfI GANTC 2 cut(s) 731, 1394
HpaII CCGG 2 cut(s) 921, 1188
HphI GGTGA 3 cut(s) 422, 556, 1435
Hpy166II GTNNAC 4 cut(s) 181, 262, 465, 1038
Hpy188I TCNGA 5 cut(s) 193, 373, 678, 838, 1027
Hpy188III TCNNGA 6 cut(s) 749, 769, 948, 1179, 1262, 1441
Hpy8I GTNNAC 4 cut(s) 181, 262, 465, 1038
HpyAV CCTTC 2 cut(s) 887, 907
HpyCH4III ACNGT 2 cut(s) 1378, 1414
HpyCH4IV ACGT 2 cut(s) 264, 273
HpyCH4V TGCA 3 cut(s) 621, 1153, 1297
HpyF10VI GCNNNNNNNGC 1 cut(s) 107
HpyF3I CTNAG 5 cut(s) 137, 351, 701, 763, 937
HpySE526I ACGT 2 cut(s) 264, 273
Hsp92II CATG 7 cut(s) 246, 395, 410, 510, 716, 731, 985
HspAI GCGC 3 cut(s) 503, 906, 1353
Kzo9I GATC 8 cut(s) 18, 328, 403, 592, 745, 808, 1184, 1431
LmnI GCTCC 2 cut(s) 133, 950
Lsp1109I GCAGC 3 cut(s) 88, 436, 1248
LweI GCATC 5 cut(s) 77, 263, 560, 955, 1114
MaeI CTAG 2 cut(s) 162, 1391
MaeII ACGT 2 cut(s) 264, 273
MaeIII GTNAC 5 cut(s) 269, 410, 994, 1132, 1250
MalI GATC 8 cut(s) 20, 330, 405, 594, 747, 810, 1186, 1433
MboI GATC 8 cut(s) 18, 328, 403, 592, 745, 808, 1184, 1431
MboII GAAGA 4 cut(s) 413, 638, 791, 1194
MfeI CAATTG 2 cut(s) 324, 1118
MflI RGATCY 1 cut(s) 1184
MhlI GDGCHC 2 cut(s) 955, 1287
MlsI TGGCCA 1 cut(s) 1071
MluCI AATT 7 cut(s) 248, 324, 356, 480, 493, 888, 1118
MluNI TGGCCA 1 cut(s) 1071
Mox20I TGGCCA 1 cut(s) 1071
MscI TGGCCA 1 cut(s) 1071
MseI TTAA 9 cut(s) 156, 206, 219, 293, 338, 440, 492, 798, 858
MslI CAYNNNNRTG 1 cut(s) 726
Msp20I TGGCCA 1 cut(s) 1071
MspI CCGG 2 cut(s) 921, 1188
MspR9I CCNGG 2 cut(s) 582, 921
MunI CAATTG 2 cut(s) 324, 1118
MvaI CCWGG 1 cut(s) 582
MvnI CGCG 2 cut(s) 906, 1382
MwoI GCNNNNNNNGC 1 cut(s) 107
NciI CCSGG 1 cut(s) 921
NcoI CCATGG 3 cut(s) 391, 506, 712
NdeII GATC 8 cut(s) 18, 328, 403, 592, 745, 808, 1184, 1431
NlaIII CATG 7 cut(s) 246, 395, 410, 510, 716, 731, 985
NlaIV GGNNCC 3 cut(s) 719, 867, 918
NmuCI GTSAC 2 cut(s) 269, 410
NspI RCATGY 1 cut(s) 985
PciI ACATGT 1 cut(s) 981
PcsI WCGNNNNNNNCGW 1 cut(s) 270
PfeI GAWTC 2 cut(s) 731, 1394
PflMI CCANNNNNTGG 1 cut(s) 875
PfoI TCCNGGA 1 cut(s) 580
PkrI GCNGC 4 cut(s) 103, 451, 847, 1238
PpuMI RGGWCCY 1 cut(s) 1300
PscI ACATGT 1 cut(s) 981
Psp124BI GAGCTC 2 cut(s) 955, 1287
Psp5II RGGWCCY 1 cut(s) 1300
Psp6I CCWGG 1 cut(s) 580
PspGI CCWGG 1 cut(s) 580
PspN4I GGNNCC 3 cut(s) 719, 867, 918
PspPI GGNCC 2 cut(s) 709, 1300
PspPPI RGGWCCY 1 cut(s) 1300
PsuI RGATCY 1 cut(s) 1184
RsaI GTAC 2 cut(s) 421, 1015
RsaNI GTAC 2 cut(s) 420, 1014
RseI CAYNNNNRTG 1 cut(s) 726
SacI GAGCTC 2 cut(s) 955, 1287
SaqAI TTAA 9 cut(s) 156, 206, 219, 293, 338, 440, 492, 798, 858
SatI GCNGC 4 cut(s) 102, 450, 846, 1237
Sau3AI GATC 8 cut(s) 18, 328, 403, 592, 745, 808, 1184, 1431
Sau96I GGNCC 2 cut(s) 709, 1300
ScaI AGTACT 1 cut(s) 421
ScrFI CCNGG 2 cut(s) 582, 921
SduI GDGCHC 2 cut(s) 955, 1287
SfaNI GCATC 5 cut(s) 77, 263, 560, 955, 1114
SfcI CTRYAG 1 cut(s) 37
SinI GGWCC 2 cut(s) 709, 1300
SmiMI CAYNNNNRTG 1 cut(s) 726
Sse9I AATT 7 cut(s) 248, 324, 356, 480, 493, 888, 1118
SsiI CCGC 5 cut(s) 332, 428, 685, 845, 1382
SspMI CTAG 2 cut(s) 162, 1391
SstI GAGCTC 2 cut(s) 955, 1287
StyD4I CCNGG 2 cut(s) 580, 919
StyI CCWWGG 3 cut(s) 391, 506, 712
TaaI ACNGT 2 cut(s) 1378, 1414
TaiI ACGT 2 cut(s) 267, 276
TaqI TCGA 4 cut(s) 302, 886, 1140, 1434
TasI AATT 7 cut(s) 248, 324, 356, 480, 493, 888, 1118
TatI WGTACW 1 cut(s) 419
TauI GCSGC 1 cut(s) 848
TfiI GAWTC 2 cut(s) 731, 1394
Tru1I TTAA 9 cut(s) 156, 206, 219, 293, 338, 440, 492, 798, 858
Tru9I TTAA 9 cut(s) 156, 206, 219, 293, 338, 440, 492, 798, 858
TscAI CASTG 2 cut(s) 352, 700
TseFI GTSAC 2 cut(s) 269, 410
TseI GCWGC 3 cut(s) 101, 449, 1236
Tsp45I GTSAC 2 cut(s) 269, 410
TspDTI ATGAA 8 cut(s) 744, 948, 992, 1061, 1093, 1125, 1233, 1327
TspGWI ACGGA 1 cut(s) 16
TspRI CASTG 2 cut(s) 352, 700
Van91I CCANNNNNTGG 1 cut(s) 875
VpaK11BI GGWCC 2 cut(s) 709, 1300
XapI RAATTY 3 cut(s) 248, 356, 480
XceI RCATGY 1 cut(s) 985
XcmI CCANNNNNNNNNTGG 2 cut(s) 513, 1007
XspI CTAG 2 cut(s) 162, 1391
ZrmI AGTACT 1 cut(s) 421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.