FvH4_3g21990

disulfide-isomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0009964
Physical Location & Seq
Reverse (-)
15034327 .. 15042596
8270 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g21990.t2

Sequence Viewer

Length: 1440 bp
ATGATTTCCTCCGGCAAGATCAAGTCGGTCGATTTCTACAGGAAAATTCCAAGAGATTTGACGGAAGCATCATTATCAGGTGCAGGATTATCTATAGTAGCAGCTCTTGCCATGATGTTTCTATTTGGAATGGAACTGAATAACTATATGACCTTCAGCACCTCTACATCTGTTATTGTTGACCAGAGTACTGATGGGGACTTCTTACGCATTGAGTTTAATATCAGTTTTCCTGCACTGTCATGTGAATTTGCATCCCTTGACGTCAGTGATGTGTTGGGAACAAACAGATTGAATATAACAAAAACCATTCGCAAGTTCTCTATAGGTCCAGATCTGAGACCCACCGGCTCTGAGTTTCAGTCTGGTCCTGCTTTACATAACATTAAGCATGGGGATGAAGGTGATGAGTATTCTGGTGATGGTTCTATTTCACTAACAGCTCATAATTTTGAAAAATTTTCTCACCACCATCCTATTCTGGTTGTCAATTTTTATGCTCCCTGGTGCTACTGGAGCAATCGCCTGAAACCTTCATGGGAGAAGGCTGCCAAAATAATGCGAGAAAGATATGATCCAGAAATAGATGGGCGTATTCTTTTGGCAAAGGTGGATTGCACTCAAGAAGCTGACTTGTGTAGGAGGAATCATATTCAAGGATATCCATCAATTCGTATTTTCCGAAGAGGAAGTGATCTAAGGGAGGACCATGGTCACCATGAGCATGAATCTTATTATGGAGATCGAGATACAGATAACTTGGTGAAGACAATGGAAGCTTTGGTTGCACCTATCCCCTTGGATTCTGAACAACTGGCGTTAGGTGGTAGCAATGAGACTGGTAATGCAAAAAGACCAGCACCATTGACAGGAGGATGTAGAATCGAAGGACATGTGCGTGTAAAGAAGGTTCCAGGAAACCTTATCATTTCAGCTCATTCAGGGACTCATTCCTTTGATGCCTCTCAAATGAATATGTCTCACTACATATCACATCTTTCATTCGGTAGAACGATTGCTCCCAAGGTGATGAGTGACGTGAAGCGATTGATACCTTATCTTGGTCGAAGTCATGAAAGGTTAAATGGTCGATCTTTTATAAATCACCGTGATATTGGAGCTAATGTCACAATAGAGCATTATCTTCAAATTGTTAAAACAGAAGTCATAACAGGGAGATCTAATAAATTGATTGAGGAATATGAGTACACTGCCCACAGTAGTTTGATACAGAGTCTGAACATCCCTGTTGCAAAATTCCATTTTGAGCTCTCCCCCATGCAGGTTTTAGTAACAGAAAATGAGAAATCCTTTTCGCATTTCATCACAAATGTGTGCGCTATCATTGGAGGCATTTTCACGGTAGCAGGAATATTGGATTCAATTCTTCACAACACCCTTAGGCTGGTGAAAAAGGTTGAACTAGGCAAAAACTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003756 GO:0003824 GO:0016853 GO:0016860 GO:0016864 GO:0140096
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
COPIIcoated_ERV ERGIC_N Thioredoxin

Protein Analysis

480

Amino Acids

53.58

Weight (kDa)

6.91

Isoelectric Point (pI)

42.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ERGIC_N PF13850 7 - 97 8.2e-23 Endoplasmic Reticulum-Golgi Intermediate Compartment (ERGIC)
Thioredoxin PF00085 144 - 254 5.4e-17 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009964)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G50950 AT3G20560 AT4G27080
fragaria_vesca FvH4_3g21990 FvH4_3g21990 FvH4_3g21990
malus_domestica MD03G1209500.v1.1 MD11G1224300.v1.1
prunus_persica Prupe.4G205600_v2.0.a1
pyrus_communis pycom03g15830 pycom11g19790 pycom11g19800
rosa_chinensis RchiOBHm_Chr5g0037841
rosa_laevigata RLG00000033816
rosa_multiflora Rmu_sc0005416.1_g000015
rosa_roxburghii Rroxscaffold_1G00043080
rosa_rugosa Rorug05G0168000 Rorug05G0168100
rosa_samantha Rh5AG256900 Rh5DG269200
rosa_wichuraiana Rw5G023820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1100
AatII GACGTC 1 cut(s) 267
Acc36I ACCTGC 1 cut(s) 1273
AclWI GGATC 1 cut(s) 569
AcsI RAATTY 4 cut(s) 45, 248, 458, 1256
AcuI CTGAAG 1 cut(s) 139
AcyI GRCGYC 1 cut(s) 264
AfaI GTAC 2 cut(s) 190, 1208
AfiI CCNNNNNNNGG 4 cut(s) 869, 1061, 1282, 1405
AflIII ACRYGT 1 cut(s) 892
AgsI TTSAA 6 cut(s) 295, 455, 656, 1148, 1383, 1421
AjiI CACGTC 1 cut(s) 1039
AjnI CCWGG 2 cut(s) 503, 913
AleI CACNNNNGTG 1 cut(s) 1331
AloI GAACNNNNNNTCC 2 cut(s) 1003, 1035
AluBI AGCT 7 cut(s) 104, 443, 629, 779, 935, 1121, 1270
AluI AGCT 7 cut(s) 104, 443, 629, 779, 935, 1121, 1270
Alw21I GWGCWC 1 cut(s) 1272
Alw26I GTCTC 3 cut(s) 334, 830, 984
AlwI GGATC 1 cut(s) 569
AlwNI CAGNNNCTG 1 cut(s) 1237
ApeKI GCWGC 2 cut(s) 101, 548
ApoI RAATTY 4 cut(s) 45, 248, 458, 1256
AspLEI GCGC 1 cut(s) 1340
AspS9I GGNCC 3 cut(s) 329, 368, 706
AsuHPI GGTGA 8 cut(s) 416, 431, 458, 707, 775, 1039, 1097, 1420
AvaII GGWCC 3 cut(s) 329, 368, 706
AxyI CCTNAGG 1 cut(s) 1400
BanII GRGCYC 1 cut(s) 1272
BbsI GAAGAC 1 cut(s) 773
Bbv12I GWGCWC 1 cut(s) 1272
BbvI GCAGC 2 cut(s) 113, 535
BccI CCATC 5 cut(s) 188, 416, 480, 581, 673
BciT130I CCWGG 2 cut(s) 505, 915
BcoDI GTCTC 3 cut(s) 334, 830, 984
BfaI CTAG 1 cut(s) 1424
BfmI CTRYAG 3 cut(s) 37, 93, 324
BfuAI ACCTGC 1 cut(s) 1273
BglII AGATCT 2 cut(s) 334, 1178
BisI GCNGC 2 cut(s) 102, 549
BlsI GCNGC 2 cut(s) 103, 550
BmcAI AGTACT 1 cut(s) 190
Bme1390I CCNGG 2 cut(s) 505, 915
Bme18I GGWCC 3 cut(s) 329, 368, 706
BmgBI CACGTC 1 cut(s) 1039
BmgT120I GGNCC 3 cut(s) 329, 368, 706
BmiI GGNNCC 1 cut(s) 912
BmrFI CCNGG 2 cut(s) 505, 915
BmsI GCATC 3 cut(s) 77, 263, 949
BoxI GACNNNNGTC 1 cut(s) 711
BpiI GAAGAC 1 cut(s) 773
BpmI CTGGAG 1 cut(s) 535
BpuEI CTTGAG 1 cut(s) 606
BsaBI GATNNNNATC 1 cut(s) 664
BsaHI GRCGYC 1 cut(s) 264
BsaI GGTCTC 1 cut(s) 334
BsaJI CCNNGG 4 cut(s) 503, 709, 798, 1023
BsaXI ACNNNNNCTCC 4 cut(s) 1003, 1033, 1110, 1140
Bsc4I CCNNNNNNNGG 4 cut(s) 869, 1061, 1282, 1405
Bse118I RCCGGY 1 cut(s) 347
Bse1I ACTGG 3 cut(s) 518, 819, 844
Bse21I CCTNAGG 1 cut(s) 1400
Bse3DI GCAATG 1 cut(s) 838
Bse8I GATNNNNATC 1 cut(s) 664
BseBI CCWGG 2 cut(s) 505, 915
BseDI CCNNGG 4 cut(s) 503, 709, 798, 1023
BseGI GGATG 5 cut(s) 254, 403, 472, 881, 1242
BseJI GATNNNNATC 1 cut(s) 664
BseLI CCNNNNNNNGG 4 cut(s) 869, 1061, 1282, 1405
BseMI GCAATG 1 cut(s) 838
BseMII CTCAG 2 cut(s) 329, 345
BseNI ACTGG 3 cut(s) 518, 819, 844
BseXI GCAGC 2 cut(s) 113, 535
BsgI GTGCAG 2 cut(s) 102, 219
Bsh1285I CGRYCG 1 cut(s) 30
BsiEI CGRYCG 1 cut(s) 30
BsiHKAI GWGCWC 1 cut(s) 1272
BsiSI CCGG 2 cut(s) 12, 348
BslFI GGGAC 2 cut(s) 212, 958
BslI CCNNNNNNNGG 4 cut(s) 869, 1061, 1282, 1405
BsmAI GTCTC 3 cut(s) 334, 830, 984
BsmFI GGGAC 2 cut(s) 212, 958
Bso31I GGTCTC 1 cut(s) 334
Bsp1286I GDGCHC 1 cut(s) 1272
Bsp143I GATC 7 cut(s) 18, 334, 574, 694, 742, 1091, 1178
Bsp19I CCATGG 1 cut(s) 709
BspCNI CTCAG 2 cut(s) 330, 346
BspHI TCATGA 1 cut(s) 1072
BspLI GGNNCC 1 cut(s) 912
BspMI ACCTGC 1 cut(s) 1273
BspPI GGATC 1 cut(s) 569
BspTNI GGTCTC 1 cut(s) 334
BsrDI GCAATG 1 cut(s) 838
BsrFI RCCGGY 1 cut(s) 347
BsrI ACTGG 3 cut(s) 518, 819, 844
BssAI RCCGGY 1 cut(s) 347
BssECI CCNNGG 4 cut(s) 503, 709, 798, 1023
BssMI GATC 7 cut(s) 18, 334, 574, 694, 742, 1091, 1178
BssNI GRCGYC 1 cut(s) 264
BssT1I CCWWGG 3 cut(s) 709, 798, 1023
Bst2UI CCWGG 2 cut(s) 505, 915
Bst4CI ACNGT 4 cut(s) 240, 1109, 1220, 1363
Bst6I CTCTTC 1 cut(s) 679
BstACI GRCGYC 1 cut(s) 264
BstAPI GCANNNNNTGC 1 cut(s) 107
BstDEI CTNAG 4 cut(s) 338, 354, 698, 1400
BstDSI CCRYGG 1 cut(s) 709
BstEII GGTNACC 1 cut(s) 713
BstF5I GGATG 5 cut(s) 254, 403, 472, 881, 1242
BstHHI GCGC 1 cut(s) 1340
BstKTI GATC 7 cut(s) 21, 337, 577, 697, 745, 1094, 1181
BstMAI GTCTC 3 cut(s) 334, 830, 984
BstMBI GATC 7 cut(s) 18, 334, 574, 694, 742, 1091, 1178
BstMCI CGRYCG 1 cut(s) 30
BstMWI GCNNNNNNNGC 3 cut(s) 107, 516, 785
BstNI CCWGG 2 cut(s) 505, 915
BstNSI RCATGY 1 cut(s) 896
BstPAI GACNNNNGTC 1 cut(s) 711
BstPI GGTNACC 1 cut(s) 713
BstSCI CCNGG 2 cut(s) 503, 913
BstSFI CTRYAG 3 cut(s) 37, 93, 324
BstV1I GCAGC 2 cut(s) 113, 535
BstV2I GAAGAC 1 cut(s) 773
BstX2I RGATCY 2 cut(s) 334, 1178
BstYI RGATCY 2 cut(s) 334, 1178
Bsu36I CCTNAGG 1 cut(s) 1400
BtgI CCRYGG 1 cut(s) 709
BtrI CACGTC 1 cut(s) 1039
BtsCI GGATG 5 cut(s) 254, 403, 472, 881, 1242
BtsI GCAGTG 1 cut(s) 1209
BtsIMutI CAGTG 3 cut(s) 236, 274, 1209
BveI ACCTGC 1 cut(s) 1273
CaiI CAGNNNCTG 1 cut(s) 1237
CciI TCATGA 1 cut(s) 1072
CfoI GCGC 1 cut(s) 1340
Cfr10I RCCGGY 1 cut(s) 347
Cfr13I GGNCC 3 cut(s) 329, 368, 706
Csp6I GTAC 2 cut(s) 189, 1207
CviQI GTAC 2 cut(s) 189, 1207
DdeI CTNAG 4 cut(s) 338, 354, 698, 1400
DpnI GATC 7 cut(s) 20, 336, 576, 696, 744, 1093, 1180
DpnII GATC 7 cut(s) 18, 334, 574, 694, 742, 1091, 1178
Eam1104I CTCTTC 1 cut(s) 679
EarI CTCTTC 1 cut(s) 679
Ecl136II GAGCTC 1 cut(s) 1270
Eco130I CCWWGG 3 cut(s) 709, 798, 1023
Eco24I GRGCYC 1 cut(s) 1272
Eco31I GGTCTC 1 cut(s) 334
Eco32I GATATC 1 cut(s) 662
Eco47I GGWCC 3 cut(s) 329, 368, 706
Eco53kI GAGCTC 1 cut(s) 1270
Eco57I CTGAAG 1 cut(s) 139
Eco81I CCTNAGG 1 cut(s) 1400
Eco91I GGTNACC 1 cut(s) 713
EcoICRI GAGCTC 1 cut(s) 1270
EcoO65I GGTNACC 1 cut(s) 713
EcoRII CCWGG 2 cut(s) 503, 913
EcoRV GATATC 1 cut(s) 662
EcoT14I CCWWGG 3 cut(s) 709, 798, 1023
EcoT38I GRGCYC 1 cut(s) 1272
ErhI CCWWGG 3 cut(s) 709, 798, 1023
FaqI GGGAC 2 cut(s) 212, 958
Fnu4HI GCNGC 2 cut(s) 102, 549
FokI GGATG 5 cut(s) 241, 410, 459, 888, 1229
FriOI GRGCYC 1 cut(s) 1272
Fsp4HI GCNGC 2 cut(s) 102, 549
FspBI CTAG 1 cut(s) 1424
GlaI GCGC 1 cut(s) 1339
GluI GCNGC 2 cut(s) 102, 549
GsuI CTGGAG 1 cut(s) 535
HapII CCGG 2 cut(s) 12, 348
HhaI GCGC 1 cut(s) 1340
Hin1I GRCGYC 1 cut(s) 264
Hin6I GCGC 1 cut(s) 1338
HinP1I GCGC 1 cut(s) 1338
HincII GTYRAC 1 cut(s) 181
HindII GTYRAC 1 cut(s) 181
HindIII AAGCTT 1 cut(s) 777
HinfI GANTC 7 cut(s) 646, 728, 803, 882, 946, 1234, 1379
HpaII CCGG 2 cut(s) 12, 348
HphI GGTGA 8 cut(s) 416, 431, 458, 707, 775, 1039, 1097, 1420
Hpy166II GTNNAC 2 cut(s) 181, 1209
Hpy188I TCNGA 6 cut(s) 339, 355, 683, 808, 1239, 1439
Hpy188III TCNNGA 5 cut(s) 332, 578, 623, 746, 1073
Hpy8I GTNNAC 2 cut(s) 181, 1209
HpyAV CCTTC 6 cut(s) 163, 395, 538, 543, 881, 901
HpyCH4III ACNGT 4 cut(s) 240, 1109, 1220, 1363
HpyCH4IV ACGT 2 cut(s) 264, 1038
HpyCH4V TGCA 8 cut(s) 83, 236, 254, 618, 788, 848, 1253, 1282
HpyF10VI GCNNNNNNNGC 3 cut(s) 107, 516, 785
HpyF3I CTNAG 4 cut(s) 338, 354, 698, 1400
HpySE526I ACGT 2 cut(s) 264, 1038
Hsp92I GRCGYC 1 cut(s) 264
HspAI GCGC 1 cut(s) 1338
Kzo9I GATC 7 cut(s) 18, 334, 574, 694, 742, 1091, 1178
LmnI GCTCC 4 cut(s) 505, 516, 1024, 1118
Lsp1109I GCAGC 2 cut(s) 113, 535
LweI GCATC 3 cut(s) 77, 263, 949
MaeI CTAG 1 cut(s) 1424
MaeII ACGT 2 cut(s) 264, 1038
MaeIII GTNAC 4 cut(s) 713, 1034, 1126, 1291
MalI GATC 7 cut(s) 20, 336, 576, 696, 744, 1093, 1180
MboI GATC 7 cut(s) 18, 334, 574, 694, 742, 1091, 1178
MboII GAAGA 4 cut(s) 696, 778, 1136, 1379
MflI RGATCY 2 cut(s) 334, 1178
MhlI GDGCHC 1 cut(s) 1272
MlyI GAGTC 2 cut(s) 940, 1243
MnlI CCTC 9 cut(s) 19, 172, 636, 680, 697, 866, 973, 1189, 1343
MseI TTAA 4 cut(s) 219, 387, 1082, 1155
MslI CAYNNNNRTG 5 cut(s) 241, 396, 723, 897, 1331
MspI CCGG 2 cut(s) 12, 348
MspR9I CCNGG 2 cut(s) 505, 915
MvaI CCWGG 2 cut(s) 505, 915
MwoI GCNNNNNNNGC 3 cut(s) 107, 516, 785
NcoI CCATGG 1 cut(s) 709
NdeII GATC 7 cut(s) 18, 334, 574, 694, 742, 1091, 1178
NlaIV GGNNCC 1 cut(s) 912
NmuCI GTSAC 3 cut(s) 713, 1034, 1126
NspI RCATGY 1 cut(s) 896
OliI CACNNNNGTG 1 cut(s) 1331
PagI TCATGA 1 cut(s) 1072
PciI ACATGT 1 cut(s) 892
PcsI WCGNNNNNNNCGW 1 cut(s) 679
PfeI GAWTC 5 cut(s) 646, 728, 803, 882, 1379
PfoI TCCNGGA 1 cut(s) 913
PkrI GCNGC 2 cut(s) 103, 550
PleI GAGTC 2 cut(s) 940, 1242
PpsI GAGTC 2 cut(s) 940, 1242
PscI ACATGT 1 cut(s) 892
PshAI GACNNNNGTC 1 cut(s) 711
PsiI TTATAA 1 cut(s) 1100
Psp124BI GAGCTC 1 cut(s) 1272
Psp6I CCWGG 2 cut(s) 503, 913
PspEI GGTNACC 1 cut(s) 713
PspGI CCWGG 2 cut(s) 503, 913
PspN4I GGNNCC 1 cut(s) 912
PspPI GGNCC 3 cut(s) 329, 368, 706
PsrI GAACNNNNNNTAC 2 cut(s) 894, 926
PstNI CAGNNNCTG 1 cut(s) 1237
PsuI RGATCY 2 cut(s) 334, 1178
RsaI GTAC 2 cut(s) 190, 1208
RsaNI GTAC 2 cut(s) 189, 1207
RseI CAYNNNNRTG 5 cut(s) 241, 396, 723, 897, 1331
SacI GAGCTC 1 cut(s) 1272
SaqAI TTAA 4 cut(s) 219, 387, 1082, 1155
SatI GCNGC 2 cut(s) 102, 549
Sau3AI GATC 7 cut(s) 18, 334, 574, 694, 742, 1091, 1178
Sau96I GGNCC 3 cut(s) 329, 368, 706
ScaI AGTACT 1 cut(s) 190
SchI GAGTC 2 cut(s) 940, 1243
ScrFI CCNGG 2 cut(s) 505, 915
SduI GDGCHC 1 cut(s) 1272
SfaNI GCATC 3 cut(s) 77, 263, 949
SfcI CTRYAG 3 cut(s) 37, 93, 324
SinI GGWCC 3 cut(s) 329, 368, 706
SmiMI CAYNNNNRTG 5 cut(s) 241, 396, 723, 897, 1331
SmlI CTYRAG 1 cut(s) 621
SmoI CTYRAG 1 cut(s) 621
SspI AATATT 1 cut(s) 1374
SspMI CTAG 1 cut(s) 1424
SstI GAGCTC 1 cut(s) 1272
StyD4I CCNGG 2 cut(s) 503, 913
StyI CCWWGG 3 cut(s) 709, 798, 1023
TaaI ACNGT 4 cut(s) 240, 1109, 1220, 1363
TaiI ACGT 2 cut(s) 267, 1041
TaqI TCGA 5 cut(s) 30, 745, 885, 1066, 1090
TaqII GACCGA 1 cut(s) 16
TatI WGTACW 2 cut(s) 188, 1206
TfiI GAWTC 5 cut(s) 646, 728, 803, 882, 1379
Tru1I TTAA 4 cut(s) 219, 387, 1082, 1155
Tru9I TTAA 4 cut(s) 219, 387, 1082, 1155
TscAI CASTG 3 cut(s) 243, 274, 1216
TseFI GTSAC 3 cut(s) 713, 1034, 1126
TseI GCWGC 2 cut(s) 101, 548
Tsp45I GTSAC 3 cut(s) 713, 1034, 1126
TspDTI ATGAA 7 cut(s) 414, 525, 741, 986, 990, 1089, 1312
TspGWI ACGGA 1 cut(s) 77
TspRI CASTG 3 cut(s) 243, 274, 1216
VpaK11BI GGWCC 3 cut(s) 329, 368, 706
XapI RAATTY 4 cut(s) 45, 248, 458, 1256
XceI RCATGY 1 cut(s) 896
XcmI CCANNNNNNNNNTGG 1 cut(s) 191
XspI CTAG 1 cut(s) 1424
ZraI GACGTC 1 cut(s) 265
ZrmI AGTACT 1 cut(s) 190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.