RchiOBHm_Chr5g0037841

disulfide-isomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0009964
Physical Location & Seq
Reverse (-)
32463792 .. 32471627
7836 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ31652

Sequence Viewer

Length: 1446 bp
ATGATTTCCTCGGGCAAGATCAAGTCGGTCGATTTCTACAGGAAAATTCCAAGAGATTTGACGGAAGCATCATTATCAGGTGCAGGATTGTCTATAGTAGCAGCTCTTGCCATGATGTTTTTATTTGGAATGGAACTGAATAATTATTTGACCTTCAGCACCTCTACATCTGTTATTGTTGACAGGAGTACTGATGGGGATTTCTTACGCATTGAGTTTAATATCAGTTTTCCTGCACTATCATGTGAATTTGCATCCCTTGACGTCAGTGATGTGTTGGGAACAAACAGATTGAATATAACAAAAACCATTCGCAAGTTCTCTATAGGTCCAGATCTGAGGCCCACCGGCTCTGAGTTTCAGTCTGGTCCTGTTTTACATAACATTAAGCATGGGGATGAAGGTGATGAGTATTCTGGTGATGGTTCTGTTTCACTAACTGCTCATAATTTTGAAAGATTTACTCAACATCATCCTATTCTGGTTGTCAATTTTCACGCTCCCTGGTGCTACTGGAGCAATCGCCTGAAACCTTCATGGGAGAAGGCTGCCAAAATAATGCGAGAAAGTTTAATATATGATCCAGAAATAGATGGGCGTATTCTTTTGGCAAAGGTGGATTGCACTGAAGAAGGTGACTTGTGTAGGAGGAATCATATTCAAGGATATCCATCGATTCGTATTTTTCGAAAAGGAAGTGATTTAAGGGAGGACCATGGGCACCATGAGCATGAATCTTATTACGGAGATCGAGATACAGATAGCTTGGTCAAGACAATGGAAGCTTTGGTTGCACCTATCCCCTTGGAGTCTCAAAAGCTGGCGTTAGGTGGTAACAATGAGACTGAGAATGCAAAAAGACCAGCACCATTGACAGGAGGATGTAGAATTGAAGGATATGTGCGTGTAAAGAAGGTTCCTGGAAACCTTATCATTTCAGCTCATTCAGGAACTCATTCCTTTGATGCCTCTCAAATGAACATGTCTCACTACATATCACATCTTTCATTCGGTAGGACAATTGCTCCTAAGGTGATGAGTGATGTGAAGCGATTGATACCTTATCTTGGTCGAAGCCATGAAAGGTTGAATGGACGATCTTTTATAAATCACCGTGATGTAGGATCTAATGTCACAATAGAGCATTATCTTCAGATAGTTAAAACGGAAGTCATAACAGGAAGATCTCACAAATTAATCGAGGAATATGAGTACACTGCCCACAGCAGTTTGATAGAGAGTCTGAACATCCCTGTTGCAAAATTCCATTTTGAGCTCTCCCCCATGCAGGTTTTAGTAACAGAAAATGAGAAATCCTTTTCGCATTTTATCACAAATGTGTGCGCTATTATTGGAGGCATTTTCACGGTAGCAGGAATATTGGATTCAATTCTGCACAACACCCTTAGGCTGGTTAAGAAGATTGAACTAGGCAAAAACTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003756 GO:0003824 GO:0016853 GO:0016860 GO:0016864 GO:0140096
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
COPIIcoated_ERV ERGIC_N Thioredoxin

Protein Analysis

481

Amino Acids

53.87

Weight (kDa)

6.77

Isoelectric Point (pI)

42.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ERGIC_N PF13850 7 - 97 1.5e-22 Endoplasmic Reticulum-Golgi Intermediate Compartment (ERGIC)
Thioredoxin PF00085 144 - 252 7.5e-16 Thioredoxin
COPIIcoated_ERV PF07970 284 - 462 9.8e-29 Endoplasmic reticulum vesicle transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009964)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G50950 AT3G20560 AT4G27080
fragaria_vesca FvH4_3g21990 FvH4_3g21990 FvH4_3g21990
malus_domestica MD03G1209500.v1.1 MD11G1224300.v1.1
prunus_persica Prupe.4G205600_v2.0.a1
pyrus_communis pycom03g15830 pycom11g19790 pycom11g19800
rosa_chinensis RchiOBHm_Chr5g0037841
rosa_laevigata RLG00000033816
rosa_multiflora Rmu_sc0005416.1_g000015
rosa_roxburghii Rroxscaffold_1G00043080
rosa_rugosa Rorug05G0168000 Rorug05G0168100
rosa_samantha Rh5AG256900 Rh5DG269200
rosa_wichuraiana Rw5G023820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1106
AatII GACGTC 1 cut(s) 267
Acc36I ACCTGC 1 cut(s) 1279
AccB1I GGYRCC 1 cut(s) 720
AclWI GGATC 2 cut(s) 575, 1132
AcsI RAATTY 3 cut(s) 45, 248, 1262
AcuI CTGAAG 3 cut(s) 139, 648, 1136
AcyI GRCGYC 1 cut(s) 264
AfaI GTAC 2 cut(s) 190, 1214
AfiI CCNNNNNNNGG 4 cut(s) 875, 1067, 1288, 1411
AflIII ACRYGT 1 cut(s) 981
AgsI TTSAA 7 cut(s) 295, 455, 662, 893, 1090, 1389, 1427
AjnI CCWGG 2 cut(s) 503, 919
AleI CACNNNNGTG 1 cut(s) 1337
AluBI AGCT 6 cut(s) 104, 765, 785, 820, 941, 1276
AluI AGCT 6 cut(s) 104, 765, 785, 820, 941, 1276
Alw21I GWGCWC 1 cut(s) 1278
Alw26I GTCTC 3 cut(s) 816, 836, 990
AlwI GGATC 2 cut(s) 575, 1132
Ama87I CYCGRG 1 cut(s) 10
AoxI GGCC 1 cut(s) 341
ApeKI GCWGC 2 cut(s) 101, 548
ApoI RAATTY 3 cut(s) 45, 248, 1262
AseI ATTAAT 1 cut(s) 1196
Asp700I GAANNNNTTC 1 cut(s) 955
AspLEI GCGC 1 cut(s) 1346
AspS9I GGNCC 4 cut(s) 329, 342, 368, 712
AsuHPI GGTGA 5 cut(s) 416, 431, 647, 1045, 1103
AsuII TTCGAA 1 cut(s) 688
AvaI CYCGRG 1 cut(s) 10
AvaII GGWCC 3 cut(s) 329, 368, 712
AxyI CCTNAGG 2 cut(s) 1029, 1406
BaeGI GKGCMC 1 cut(s) 723
BanI GGYRCC 1 cut(s) 720
BanII GRGCYC 1 cut(s) 1278
Bbv12I GWGCWC 1 cut(s) 1278
BbvI GCAGC 2 cut(s) 113, 535
BccI CCATC 4 cut(s) 188, 416, 587, 679
BciT130I CCWGG 2 cut(s) 505, 921
BcoDI GTCTC 3 cut(s) 816, 836, 990
BfaI CTAG 1 cut(s) 1430
BfmI CTRYAG 3 cut(s) 37, 93, 324
BfuAI ACCTGC 1 cut(s) 1279
BglII AGATCT 2 cut(s) 334, 1184
BisI GCNGC 2 cut(s) 102, 549
BlsI GCNGC 2 cut(s) 103, 550
BmcAI AGTACT 1 cut(s) 190
Bme1390I CCNGG 2 cut(s) 505, 921
Bme18I GGWCC 3 cut(s) 329, 368, 712
BmeT110I CYCGRG 1 cut(s) 10
BmgT120I GGNCC 4 cut(s) 329, 342, 368, 712
BmiI GGNNCC 2 cut(s) 722, 918
BmrFI CCNGG 2 cut(s) 505, 921
BmsI GCATC 3 cut(s) 77, 263, 955
BpmI CTGGAG 1 cut(s) 535
Bpu14I TTCGAA 1 cut(s) 688
Bsa29I ATCGAT 1 cut(s) 674
BsaBI GATNNNNATC 1 cut(s) 670
BsaHI GRCGYC 1 cut(s) 264
BsaJI CCNNGG 4 cut(s) 9, 503, 715, 804
BsaXI ACNNNNNCTCC 2 cut(s) 1009, 1039
Bsc4I CCNNNNNNNGG 4 cut(s) 875, 1067, 1288, 1411
Bse118I RCCGGY 1 cut(s) 347
Bse1I ACTGG 1 cut(s) 518
Bse21I CCTNAGG 2 cut(s) 1029, 1406
Bse8I GATNNNNATC 1 cut(s) 670
BseBI CCWGG 2 cut(s) 505, 921
BseCI ATCGAT 1 cut(s) 674
BseDI CCNNGG 4 cut(s) 9, 503, 715, 804
BseGI GGATG 5 cut(s) 254, 403, 472, 887, 1248
BseJI GATNNNNATC 1 cut(s) 670
BseLI CCNNNNNNNGG 4 cut(s) 875, 1067, 1288, 1411
BseMII CTCAG 3 cut(s) 329, 345, 837
BseNI ACTGG 1 cut(s) 518
BseSI GKGCMC 1 cut(s) 723
BseXI GCAGC 2 cut(s) 113, 535
BsgI GTGCAG 3 cut(s) 102, 219, 1379
Bsh1285I CGRYCG 1 cut(s) 30
BshFI GGCC 1 cut(s) 343
BshNI GGYRCC 1 cut(s) 720
BshVI ATCGAT 1 cut(s) 674
BsiEI CGRYCG 1 cut(s) 30
BsiHKAI GWGCWC 1 cut(s) 1278
BsiHKCI CYCGRG 1 cut(s) 10
BsiSI CCGG 1 cut(s) 348
BslI CCNNNNNNNGG 4 cut(s) 875, 1067, 1288, 1411
BsmAI GTCTC 3 cut(s) 816, 836, 990
BsmI GAATGC 1 cut(s) 856
BsnI GGCC 1 cut(s) 343
BsoBI CYCGRG 1 cut(s) 10
Bsp119I TTCGAA 1 cut(s) 688
Bsp1286I GDGCHC 2 cut(s) 723, 1278
Bsp143I GATC 7 cut(s) 18, 334, 580, 748, 1097, 1124, 1184
Bsp19I CCATGG 1 cut(s) 715
BspANI GGCC 1 cut(s) 343
BspCNI CTCAG 3 cut(s) 330, 346, 838
BspDI ATCGAT 1 cut(s) 674
BspLI GGNNCC 2 cut(s) 722, 918
BspMI ACCTGC 1 cut(s) 1279
BspPI GGATC 2 cut(s) 575, 1132
BspT104I TTCGAA 1 cut(s) 688
BspT107I GGYRCC 1 cut(s) 720
BsrFI RCCGGY 1 cut(s) 347
BsrI ACTGG 1 cut(s) 518
BssAI RCCGGY 1 cut(s) 347
BssECI CCNNGG 4 cut(s) 9, 503, 715, 804
BssMI GATC 7 cut(s) 18, 334, 580, 748, 1097, 1124, 1184
BssNI GRCGYC 1 cut(s) 264
BssT1I CCWWGG 2 cut(s) 715, 804
Bst2UI CCWGG 2 cut(s) 505, 921
Bst4CI ACNGT 2 cut(s) 1115, 1369
BstACI GRCGYC 1 cut(s) 264
BstAPI GCANNNNNTGC 1 cut(s) 107
BstBI TTCGAA 1 cut(s) 688
BstC8I GCNNGC 1 cut(s) 822
BstDEI CTNAG 5 cut(s) 338, 354, 846, 1029, 1406
BstDSI CCRYGG 1 cut(s) 715
BstF5I GGATG 5 cut(s) 254, 403, 472, 887, 1248
BstHHI GCGC 1 cut(s) 1346
BstKTI GATC 7 cut(s) 21, 337, 583, 751, 1100, 1127, 1187
BstMAI GTCTC 3 cut(s) 816, 836, 990
BstMBI GATC 7 cut(s) 18, 334, 580, 748, 1097, 1124, 1184
BstMCI CGRYCG 1 cut(s) 30
BstMWI GCNNNNNNNGC 4 cut(s) 107, 516, 727, 791
BstNI CCWGG 2 cut(s) 505, 921
BstNSI RCATGY 1 cut(s) 985
BstSCI CCNGG 2 cut(s) 503, 919
BstSFI CTRYAG 3 cut(s) 37, 93, 324
BstSLI GKGCMC 1 cut(s) 723
BstV1I GCAGC 2 cut(s) 113, 535
BstX2I RGATCY 3 cut(s) 334, 1124, 1184
BstYI RGATCY 3 cut(s) 334, 1124, 1184
Bsu15I ATCGAT 1 cut(s) 674
Bsu36I CCTNAGG 2 cut(s) 1029, 1406
BsuRI GGCC 1 cut(s) 343
BsuTUI ATCGAT 1 cut(s) 674
BtgI CCRYGG 1 cut(s) 715
BtsCI GGATG 5 cut(s) 254, 403, 472, 887, 1248
BtsI GCAGTG 1 cut(s) 1215
BtsIMutI CAGTG 3 cut(s) 274, 624, 1215
BveI ACCTGC 1 cut(s) 1279
Cac8I GCNNGC 1 cut(s) 822
CfoI GCGC 1 cut(s) 1346
Cfr10I RCCGGY 1 cut(s) 347
Cfr13I GGNCC 4 cut(s) 329, 342, 368, 712
ClaI ATCGAT 1 cut(s) 674
Csp6I GTAC 2 cut(s) 189, 1213
CviQI GTAC 2 cut(s) 189, 1213
DdeI CTNAG 5 cut(s) 338, 354, 846, 1029, 1406
DpnI GATC 7 cut(s) 20, 336, 582, 750, 1099, 1126, 1186
DpnII GATC 7 cut(s) 18, 334, 580, 748, 1097, 1124, 1184
Ecl136II GAGCTC 1 cut(s) 1276
Eco130I CCWWGG 2 cut(s) 715, 804
Eco24I GRGCYC 1 cut(s) 1278
Eco32I GATATC 1 cut(s) 668
Eco47I GGWCC 3 cut(s) 329, 368, 712
Eco53kI GAGCTC 1 cut(s) 1276
Eco57I CTGAAG 3 cut(s) 139, 648, 1136
Eco81I CCTNAGG 2 cut(s) 1029, 1406
Eco88I CYCGRG 1 cut(s) 10
EcoICRI GAGCTC 1 cut(s) 1276
EcoRII CCWGG 2 cut(s) 503, 919
EcoRV GATATC 1 cut(s) 668
EcoT14I CCWWGG 2 cut(s) 715, 804
EcoT38I GRGCYC 1 cut(s) 1278
ErhI CCWWGG 2 cut(s) 715, 804
Fnu4HI GCNGC 2 cut(s) 102, 549
FokI GGATG 5 cut(s) 241, 410, 459, 894, 1235
FriOI GRGCYC 1 cut(s) 1278
Fsp4HI GCNGC 2 cut(s) 102, 549
FspBI CTAG 1 cut(s) 1430
GlaI GCGC 1 cut(s) 1345
GluI GCNGC 2 cut(s) 102, 549
GsuI CTGGAG 1 cut(s) 535
HaeIII GGCC 1 cut(s) 343
HapII CCGG 1 cut(s) 348
HhaI GCGC 1 cut(s) 1346
Hin1I GRCGYC 1 cut(s) 264
Hin6I GCGC 1 cut(s) 1344
HinP1I GCGC 1 cut(s) 1344
HincII GTYRAC 1 cut(s) 181
HindII GTYRAC 1 cut(s) 181
HindIII AAGCTT 1 cut(s) 783
HinfI GANTC 6 cut(s) 652, 676, 734, 809, 1240, 1385
HpaII CCGG 1 cut(s) 348
HphI GGTGA 5 cut(s) 416, 431, 647, 1045, 1103
Hpy166II GTNNAC 2 cut(s) 181, 1215
Hpy188I TCNGA 4 cut(s) 339, 355, 1155, 1245
Hpy188III TCNNGA 5 cut(s) 332, 584, 752, 772, 948
Hpy8I GTNNAC 2 cut(s) 181, 1215
HpyAV CCTTC 7 cut(s) 163, 395, 538, 543, 626, 887, 907
HpyCH4III ACNGT 2 cut(s) 1115, 1369
HpyCH4IV ACGT 1 cut(s) 264
HpyCH4V TGCA 9 cut(s) 83, 236, 254, 624, 794, 854, 1259, 1288, 1396
HpyF10VI GCNNNNNNNGC 4 cut(s) 107, 516, 727, 791
HpyF3I CTNAG 5 cut(s) 338, 354, 846, 1029, 1406
HpySE526I ACGT 1 cut(s) 264
Hsp92I GRCGYC 1 cut(s) 264
HspAI GCGC 1 cut(s) 1344
Kzo9I GATC 7 cut(s) 18, 334, 580, 748, 1097, 1124, 1184
LmnI GCTCC 3 cut(s) 505, 516, 1030
Lsp1109I GCAGC 2 cut(s) 113, 535
LweI GCATC 3 cut(s) 77, 263, 955
MaeI CTAG 1 cut(s) 1430
MaeII ACGT 1 cut(s) 264
MaeIII GTNAC 4 cut(s) 635, 833, 1132, 1297
MalI GATC 7 cut(s) 20, 336, 582, 750, 1099, 1126, 1186
MboI GATC 7 cut(s) 18, 334, 580, 748, 1097, 1124, 1184
MboII GAAGA 4 cut(s) 641, 1142, 1194, 1432
MfeI CAATTG 1 cut(s) 1020
MflI RGATCY 3 cut(s) 334, 1124, 1184
MhlI GDGCHC 2 cut(s) 723, 1278
MlyI GAGTC 2 cut(s) 818, 1249
MnlI CCTC 9 cut(s) 19, 172, 333, 642, 703, 872, 979, 1195, 1349
MroXI GAANNNNTTC 1 cut(s) 955
MseI TTAA 7 cut(s) 219, 387, 572, 704, 1161, 1196, 1416
MslI CAYNNNNRTG 5 cut(s) 241, 396, 729, 1116, 1337
MspI CCGG 1 cut(s) 348
MspR9I CCNGG 2 cut(s) 505, 921
MunI CAATTG 1 cut(s) 1020
Mva1269I GAATGC 1 cut(s) 856
MvaI CCWGG 2 cut(s) 505, 921
MwoI GCNNNNNNNGC 4 cut(s) 107, 516, 727, 791
NcoI CCATGG 1 cut(s) 715
NdeII GATC 7 cut(s) 18, 334, 580, 748, 1097, 1124, 1184
NlaIV GGNNCC 2 cut(s) 722, 918
NmuCI GTSAC 2 cut(s) 635, 1132
NspI RCATGY 1 cut(s) 985
NspV TTCGAA 1 cut(s) 688
OliI CACNNNNGTG 1 cut(s) 1337
PciI ACATGT 1 cut(s) 981
PcsI WCGNNNNNNNCGW 1 cut(s) 685
PctI GAATGC 1 cut(s) 856
PdmI GAANNNNTTC 1 cut(s) 955
PfeI GAWTC 4 cut(s) 652, 676, 734, 1385
PfoI TCCNGGA 1 cut(s) 919
PkrI GCNGC 2 cut(s) 103, 550
PleI GAGTC 2 cut(s) 817, 1248
PpsI GAGTC 2 cut(s) 817, 1248
PscI ACATGT 1 cut(s) 981
PshBI ATTAAT 1 cut(s) 1196
PsiI TTATAA 1 cut(s) 1106
Psp124BI GAGCTC 1 cut(s) 1278
Psp6I CCWGG 2 cut(s) 503, 919
PspGI CCWGG 2 cut(s) 503, 919
PspN4I GGNNCC 2 cut(s) 722, 918
PspPI GGNCC 4 cut(s) 329, 342, 368, 712
PsrI GAACNNNNNNTAC 2 cut(s) 900, 932
PsuI RGATCY 3 cut(s) 334, 1124, 1184
RsaI GTAC 2 cut(s) 190, 1214
RsaNI GTAC 2 cut(s) 189, 1213
RseI CAYNNNNRTG 5 cut(s) 241, 396, 729, 1116, 1337
SacI GAGCTC 1 cut(s) 1278
SaqAI TTAA 7 cut(s) 219, 387, 572, 704, 1161, 1196, 1416
SatI GCNGC 2 cut(s) 102, 549
Sau3AI GATC 7 cut(s) 18, 334, 580, 748, 1097, 1124, 1184
Sau96I GGNCC 4 cut(s) 329, 342, 368, 712
ScaI AGTACT 1 cut(s) 190
SchI GAGTC 2 cut(s) 818, 1249
ScrFI CCNGG 2 cut(s) 505, 921
SduI GDGCHC 2 cut(s) 723, 1278
SfaNI GCATC 3 cut(s) 77, 263, 955
SfcI CTRYAG 3 cut(s) 37, 93, 324
SfuI TTCGAA 1 cut(s) 688
SinI GGWCC 3 cut(s) 329, 368, 712
SmiMI CAYNNNNRTG 5 cut(s) 241, 396, 729, 1116, 1337
SspI AATATT 1 cut(s) 1380
SspMI CTAG 1 cut(s) 1430
SstI GAGCTC 1 cut(s) 1278
StyD4I CCNGG 2 cut(s) 503, 919
StyI CCWWGG 2 cut(s) 715, 804
TaaI ACNGT 2 cut(s) 1115, 1369
TaiI ACGT 1 cut(s) 267
TaqI TCGA 6 cut(s) 30, 674, 688, 751, 1072, 1200
TaqII GACCGA 1 cut(s) 16
TatI WGTACW 2 cut(s) 188, 1212
TfiI GAWTC 4 cut(s) 652, 676, 734, 1385
Tru1I TTAA 7 cut(s) 219, 387, 572, 704, 1161, 1196, 1416
Tru9I TTAA 7 cut(s) 219, 387, 572, 704, 1161, 1196, 1416
TscAI CASTG 3 cut(s) 274, 631, 1222
TseFI GTSAC 2 cut(s) 635, 1132
TseI GCWGC 2 cut(s) 101, 548
Tsp45I GTSAC 2 cut(s) 635, 1132
TspDTI ATGAA 6 cut(s) 414, 525, 747, 992, 996, 1095
TspGWI ACGGA 3 cut(s) 77, 759, 1181
TspRI CASTG 3 cut(s) 274, 631, 1222
VpaK11BI GGWCC 3 cut(s) 329, 368, 712
VspI ATTAAT 1 cut(s) 1196
XapI RAATTY 3 cut(s) 45, 248, 1262
XceI RCATGY 1 cut(s) 985
XmnI GAANNNNTTC 1 cut(s) 955
XspI CTAG 1 cut(s) 1430
ZraI GACGTC 1 cut(s) 265
ZrmI AGTACT 1 cut(s) 190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.