pycom03g15830

disulfide-isomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0009964
Physical Location & Seq
Forward (+)
17077441 .. 17085561
8121 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g15830.11

Sequence Viewer

Length: 1443 bp
ATGATTTCCACCGGCAAGATCAAGTCCGTTGATTTTTACAGGAAAATTCCTAGAGATTTGACAGAAGCATCATTATCCGGTGCAGGATTATCTATAATAGCAGCTCTTGCCATGATGTTTTTATTTGGAATGGAACTGAATAATTATTTGACTGTCAGCACCTCTACATCTGTTATTGTTGACAAGAGTTCTGATGGGGACTTCTTACGCATTGAATTTAATGTCAGTTTTCCCGCACTCTCATGTGAATTTGCATCCGTTGACGTGAGTGATGTGTTGGGAACAAACAGATTGAATATAACAAAAACAATTCGCAAGTTCTCTATAGGGACAGATTTAAGACCAACTGGCTCCGAGTTCCGCTCCGGACCCGTTTTACATAACATTAAGCATGGGGATGGAGATGAGTATTCTGGTGATGGCTCTATTTCGCTAACTGCCCAGAATTTTGAAAAGTTTACTCACCAGCACCCAATTTTGATTGTTAATTTTTATGCTCCTTGGTGCTACTGGAGCAATCGCCTGAAACCTTCATGGGAGAAGGCTGCCAAAATAATAAGAGAAAGGTATGATCCAGAAATAGATGGTCGTATTCTTATGGCGAAGGTGGATTGTACTGAAGAAGTTGACTTGTGTAGGAGGAATCACATTCAAGGATATCCATCAATTCGTATTTTTCGTAAAGGAAGTGATGTGAGGGATGAGCATGGACACCATGAGCATGAATCTTATTATGGAGATCGAGATACAGATAGCCTGGTTAAGACAATGGAAGCTTTGGTTGCACCTATCCCTGTGGAGTCTGAAAAACTCTCTTTGGAGGATAAATCTGACAACAGGGGTGAGAATGCAAAAAGACAAGCACCATTGACAGGAGGGTGTAGAATTGAAGGATATGTGCGTGTAAAGAAGGTTCCAGGAAACCTTGTCATTTCTGCACATTCAGGAGCCCATTCGTTTGATGCTTCTCAAATGAACATGTCTCACGTCATATCACATTTTTCATTCGGTAGGATGATTGCTCCTAAGGTGATGAGTGATGTCAAGCGGTTGGTACCTTATCTTGGTGGAAGCCATGATAGGTTGAATGGTCGGTCTTTCATAAATCACCAGGAGTTAGGTGCTAATGTTACAATAGAGCATTACCTTCAAATAGTAAAATCGGAAGTTATAACAGGGAGATCTCACAAATCAATTGAGGAATACGAGTATACGGCCCACAGCAGCTTGGTGCAAAGTCTCCAAGTACCAGTTGCGAAGTTCCATTTTGAGCTCTCCCCCATGCAGGTTTTAATAACAGAAAACCAGAAATCCTTTTCACATTTTATCACCAATGTTTGCGCTATTATCGGAGGCGTTTTCACGGTAGCGGGGATAATGGATTCAATTCTGCACAACACAATTAGAATGATGAGGAAGGTTGAACTAGGCAAAAACTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003756 GO:0003824 GO:0016853 GO:0016860 GO:0016864 GO:0140096
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
COPIIcoated_ERV ERGIC_N Thioredoxin

Protein Analysis

481

Amino Acids

53.78

Weight (kDa)

7.07

Isoelectric Point (pI)

35.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009964)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G50950 AT3G20560 AT4G27080
fragaria_vesca FvH4_3g21990 FvH4_3g21990 FvH4_3g21990
malus_domestica MD03G1209500.v1.1 MD11G1224300.v1.1
prunus_persica Prupe.4G205600_v2.0.a1
pyrus_communis pycom03g15830 pycom11g19790 pycom11g19800
rosa_chinensis RchiOBHm_Chr5g0037841
rosa_laevigata RLG00000033816
rosa_multiflora Rmu_sc0005416.1_g000015
rosa_roxburghii Rroxscaffold_1G00043080
rosa_rugosa Rorug05G0168000 Rorug05G0168100
rosa_samantha Rh5AG256900 Rh5DG269200
rosa_wichuraiana Rw5G023820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1172
Acc36I ACCTGC 1 cut(s) 1276
Acc65I GGTACC 1 cut(s) 1054
AccB1I GGYRCC 1 cut(s) 1054
AccBSI CCGCTC 1 cut(s) 363
AccI GTMKAC 1 cut(s) 1211
AccIII TCCGGA 1 cut(s) 365
AciI CCGC 4 cut(s) 234, 361, 1048, 1370
AclWI GGATC 1 cut(s) 566
AcsI RAATTY 4 cut(s) 45, 215, 248, 445
AcuI CTGAAG 1 cut(s) 639
AfaI GTAC 3 cut(s) 616, 1056, 1248
AfiI CCNNNNNNNGG 3 cut(s) 872, 1064, 1285
AflIII ACRYGT 1 cut(s) 978
AgsI TTSAA 9 cut(s) 215, 295, 452, 653, 890, 1087, 1151, 1386, 1424
AjiI CACGTC 2 cut(s) 265, 988
AjnI CCWGG 3 cut(s) 756, 916, 1110
AluBI AGCT 4 cut(s) 104, 776, 1227, 1273
AluI AGCT 4 cut(s) 104, 776, 1227, 1273
Alw21I GWGCWC 1 cut(s) 1275
Alw26I GTCTC 2 cut(s) 987, 1244
AlwI GGATC 1 cut(s) 566
Aor13HI TCCGGA 1 cut(s) 365
AoxI GGCC 1 cut(s) 1215
ApeKI GCWGC 3 cut(s) 101, 545, 1224
ApoI RAATTY 4 cut(s) 45, 215, 248, 445
Asp718I GGTACC 1 cut(s) 1054
AspLEI GCGC 1 cut(s) 1343
AspS9I GGNCC 2 cut(s) 368, 1216
AsuHPI GGTGA 6 cut(s) 428, 455, 854, 1042, 1100, 1321
AvaII GGWCC 1 cut(s) 368
AxyI CCTNAGG 1 cut(s) 1026
BanI GGYRCC 1 cut(s) 1054
BanII GRGCYC 2 cut(s) 952, 1275
Bbv12I GWGCWC 1 cut(s) 1275
BbvI GCAGC 3 cut(s) 113, 532, 1236
BccI CCATC 5 cut(s) 188, 392, 413, 578, 670
BceAI ACGGC 1 cut(s) 1230
BciT130I CCWGG 3 cut(s) 758, 918, 1112
BcoDI GTCTC 2 cut(s) 987, 1244
BfaI CTAG 2 cut(s) 51, 1427
BfmI CTRYAG 1 cut(s) 324
BfuAI ACCTGC 1 cut(s) 1276
BglII AGATCT 1 cut(s) 1181
BisI GCNGC 3 cut(s) 102, 546, 1225
BlsI GCNGC 3 cut(s) 103, 547, 1226
Bme1390I CCNGG 3 cut(s) 758, 918, 1112
Bme18I GGWCC 1 cut(s) 368
BmgBI CACGTC 2 cut(s) 265, 988
BmgT120I GGNCC 2 cut(s) 368, 1216
BmiI GGNNCC 5 cut(s) 352, 370, 915, 949, 1056
BmrFI CCNGG 3 cut(s) 758, 918, 1112
BmsI GCATC 3 cut(s) 77, 263, 952
BplI GAGNNNNNCTC 2 cut(s) 347, 379
BpmI CTGGAG 1 cut(s) 532
BsaBI GATNNNNATC 1 cut(s) 661
BsaJI CCNNGG 1 cut(s) 500
BsaWI WCCGGW 2 cut(s) 77, 365
Bsc4I CCNNNNNNNGG 3 cut(s) 872, 1064, 1285
Bse118I RCCGGY 1 cut(s) 11
Bse1I ACTGG 3 cut(s) 352, 515, 1250
Bse21I CCTNAGG 1 cut(s) 1026
Bse8I GATNNNNATC 1 cut(s) 661
BseAI TCCGGA 1 cut(s) 365
BseBI CCWGG 3 cut(s) 758, 918, 1112
BseDI CCNNGG 1 cut(s) 500
BseGI GGATG 4 cut(s) 254, 403, 706, 1020
BseJI GATNNNNATC 1 cut(s) 661
BseLI CCNNNNNNNGG 3 cut(s) 872, 1064, 1285
BseNI ACTGG 3 cut(s) 352, 515, 1250
BseXI GCAGC 3 cut(s) 113, 532, 1236
BsgI GTGCAG 3 cut(s) 102, 921, 1376
BshFI GGCC 1 cut(s) 1217
BshNI GGYRCC 1 cut(s) 1054
BsiHKAI GWGCWC 1 cut(s) 1275
BsiSI CCGG 3 cut(s) 12, 78, 366
BslFI GGGAC 2 cut(s) 212, 343
BslI CCNNNNNNNGG 3 cut(s) 872, 1064, 1285
BsmAI GTCTC 2 cut(s) 987, 1244
BsmFI GGGAC 2 cut(s) 212, 343
BsmI GAATGC 1 cut(s) 853
BsnI GGCC 1 cut(s) 1217
Bsp1286I GDGCHC 2 cut(s) 952, 1275
Bsp13I TCCGGA 1 cut(s) 365
Bsp143I GATC 4 cut(s) 18, 571, 739, 1181
BspACI CCGC 4 cut(s) 234, 361, 1048, 1370
BspANI GGCC 1 cut(s) 1217
BspEI TCCGGA 1 cut(s) 365
BspLI GGNNCC 5 cut(s) 352, 370, 915, 949, 1056
BspMI ACCTGC 1 cut(s) 1276
BspPI GGATC 1 cut(s) 566
BspT107I GGYRCC 1 cut(s) 1054
BsrBI CCGCTC 1 cut(s) 363
BsrFI RCCGGY 1 cut(s) 11
BsrI ACTGG 3 cut(s) 352, 515, 1250
BssAI RCCGGY 1 cut(s) 11
BssECI CCNNGG 1 cut(s) 500
BssMI GATC 4 cut(s) 18, 571, 739, 1181
BssNAI GTATAC 1 cut(s) 1212
BssT1I CCWWGG 1 cut(s) 500
Bst1107I GTATAC 1 cut(s) 1212
Bst2UI CCWGG 3 cut(s) 758, 918, 1112
Bst4CI ACNGT 2 cut(s) 154, 1366
BstAPI GCANNNNNTGC 1 cut(s) 107
BstDEI CTNAG 1 cut(s) 1026
BstF5I GGATG 4 cut(s) 254, 403, 706, 1020
BstHHI GCGC 1 cut(s) 1343
BstKTI GATC 4 cut(s) 21, 574, 742, 1184
BstMAI GTCTC 2 cut(s) 987, 1244
BstMBI GATC 4 cut(s) 18, 571, 739, 1181
BstMWI GCNNNNNNNGC 3 cut(s) 107, 513, 782
BstNI CCWGG 3 cut(s) 758, 918, 1112
BstNSI RCATGY 1 cut(s) 982
BstSCI CCNGG 3 cut(s) 756, 916, 1110
BstSFI CTRYAG 1 cut(s) 324
BstV1I GCAGC 3 cut(s) 113, 532, 1236
BstX2I RGATCY 1 cut(s) 1181
BstYI RGATCY 1 cut(s) 1181
BstZ17I GTATAC 1 cut(s) 1212
Bsu36I CCTNAGG 1 cut(s) 1026
BsuRI GGCC 1 cut(s) 1217
BtrI CACGTC 2 cut(s) 265, 988
BtsCI GGATG 4 cut(s) 254, 403, 706, 1020
BveI ACCTGC 1 cut(s) 1276
CfoI GCGC 1 cut(s) 1343
Cfr10I RCCGGY 1 cut(s) 11
Cfr13I GGNCC 2 cut(s) 368, 1216
Csp6I GTAC 3 cut(s) 615, 1055, 1247
CviQI GTAC 3 cut(s) 615, 1055, 1247
DdeI CTNAG 1 cut(s) 1026
DpnI GATC 4 cut(s) 20, 573, 741, 1183
DpnII GATC 4 cut(s) 18, 571, 739, 1181
Ecl136II GAGCTC 1 cut(s) 1273
Eco130I CCWWGG 1 cut(s) 500
Eco24I GRGCYC 2 cut(s) 952, 1275
Eco32I GATATC 1 cut(s) 659
Eco47I GGWCC 1 cut(s) 368
Eco53kI GAGCTC 1 cut(s) 1273
Eco57I CTGAAG 1 cut(s) 639
Eco81I CCTNAGG 1 cut(s) 1026
EcoICRI GAGCTC 1 cut(s) 1273
EcoRII CCWGG 3 cut(s) 756, 916, 1110
EcoRV GATATC 1 cut(s) 659
EcoT14I CCWWGG 1 cut(s) 500
EcoT38I GRGCYC 2 cut(s) 952, 1275
ErhI CCWWGG 1 cut(s) 500
FaqI GGGAC 2 cut(s) 212, 343
FauI CCCGC 2 cut(s) 241, 1363
FblI GTMKAC 1 cut(s) 1211
Fnu4HI GCNGC 3 cut(s) 102, 546, 1225
FokI GGATG 4 cut(s) 241, 410, 713, 1027
FriOI GRGCYC 2 cut(s) 952, 1275
Fsp4HI GCNGC 3 cut(s) 102, 546, 1225
FspBI CTAG 2 cut(s) 51, 1427
GlaI GCGC 1 cut(s) 1342
GluI GCNGC 3 cut(s) 102, 546, 1225
GsuI CTGGAG 1 cut(s) 532
HaeIII GGCC 1 cut(s) 1217
HapII CCGG 3 cut(s) 12, 78, 366
HhaI GCGC 1 cut(s) 1343
Hin6I GCGC 1 cut(s) 1341
HinP1I GCGC 1 cut(s) 1341
HincII GTYRAC 3 cut(s) 181, 262, 628
HindII GTYRAC 3 cut(s) 181, 262, 628
HindIII AAGCTT 1 cut(s) 774
HinfI GANTC 4 cut(s) 643, 725, 800, 1382
HpaII CCGG 3 cut(s) 12, 78, 366
HphI GGTGA 6 cut(s) 428, 455, 854, 1042, 1100, 1321
Hpy166II GTNNAC 5 cut(s) 181, 262, 459, 628, 1212
Hpy188I TCNGA 6 cut(s) 193, 355, 805, 832, 1165, 1352
Hpy188III TCNNGA 4 cut(s) 366, 575, 743, 945
Hpy8I GTNNAC 5 cut(s) 181, 262, 459, 628, 1212
HpyAV CCTTC 7 cut(s) 535, 540, 598, 884, 904, 1157, 1411
HpyCH4III ACNGT 2 cut(s) 154, 1366
HpyCH4IV ACGT 2 cut(s) 264, 987
HpyCH4V TGCA 8 cut(s) 83, 254, 785, 851, 938, 1234, 1285, 1393
HpyF10VI GCNNNNNNNGC 3 cut(s) 107, 513, 782
HpyF3I CTNAG 1 cut(s) 1026
HpySE526I ACGT 2 cut(s) 264, 987
HspAI GCGC 1 cut(s) 1341
Kpn2I TCCGGA 1 cut(s) 365
KpnI GGTACC 1 cut(s) 1058
Kzo9I GATC 4 cut(s) 18, 571, 739, 1181
LmnI GCTCC 6 cut(s) 356, 368, 502, 513, 947, 1027
Lsp1109I GCAGC 3 cut(s) 113, 532, 1236
LweI GCATC 3 cut(s) 77, 263, 952
MaeI CTAG 2 cut(s) 51, 1427
MaeII ACGT 2 cut(s) 264, 987
MaeIII GTNAC 1 cut(s) 1129
MalI GATC 4 cut(s) 20, 573, 741, 1183
MbiI CCGCTC 1 cut(s) 363
MboI GATC 4 cut(s) 18, 571, 739, 1181
MboII GAAGA 1 cut(s) 632
MfeI CAATTG 1 cut(s) 1194
MflI RGATCY 1 cut(s) 1181
MhlI GDGCHC 2 cut(s) 952, 1275
MlyI GAGTC 1 cut(s) 809
MnlI CCTC 8 cut(s) 172, 633, 690, 814, 869, 1192, 1346, 1407
MroI TCCGGA 1 cut(s) 365
MseI TTAA 6 cut(s) 219, 338, 387, 486, 762, 1292
MslI CAYNNNNRTG 3 cut(s) 241, 396, 720
MspI CCGG 3 cut(s) 12, 78, 366
MspR9I CCNGG 3 cut(s) 758, 918, 1112
MunI CAATTG 1 cut(s) 1194
Mva1269I GAATGC 1 cut(s) 853
MvaI CCWGG 3 cut(s) 758, 918, 1112
MwoI GCNNNNNNNGC 3 cut(s) 107, 513, 782
NdeII GATC 4 cut(s) 18, 571, 739, 1181
NlaIV GGNNCC 5 cut(s) 352, 370, 915, 949, 1056
NspI RCATGY 1 cut(s) 982
PciI ACATGT 1 cut(s) 978
PcsI WCGNNNNNNNCGW 1 cut(s) 676
PctI GAATGC 1 cut(s) 853
PfeI GAWTC 3 cut(s) 643, 725, 1382
PfoI TCCNGGA 1 cut(s) 916
PkrI GCNGC 3 cut(s) 103, 547, 1226
PleI GAGTC 1 cut(s) 808
PpsI GAGTC 1 cut(s) 808
PscI ACATGT 1 cut(s) 978
PsiI TTATAA 1 cut(s) 1172
Psp124BI GAGCTC 1 cut(s) 1275
Psp6I CCWGG 3 cut(s) 756, 916, 1110
PspGI CCWGG 3 cut(s) 756, 916, 1110
PspN4I GGNNCC 5 cut(s) 352, 370, 915, 949, 1056
PspPI GGNCC 2 cut(s) 368, 1216
PsrI GAACNNNNNNTAC 2 cut(s) 897, 929
PsuI RGATCY 1 cut(s) 1181
RsaI GTAC 3 cut(s) 616, 1056, 1248
RsaNI GTAC 3 cut(s) 615, 1055, 1247
RseI CAYNNNNRTG 3 cut(s) 241, 396, 720
SacI GAGCTC 1 cut(s) 1275
SaqAI TTAA 6 cut(s) 219, 338, 387, 486, 762, 1292
SatI GCNGC 3 cut(s) 102, 546, 1225
Sau3AI GATC 4 cut(s) 18, 571, 739, 1181
Sau96I GGNCC 2 cut(s) 368, 1216
SchI GAGTC 1 cut(s) 809
ScrFI CCNGG 3 cut(s) 758, 918, 1112
SduI GDGCHC 2 cut(s) 952, 1275
SfaNI GCATC 3 cut(s) 77, 263, 952
SfcI CTRYAG 1 cut(s) 324
SinI GGWCC 1 cut(s) 368
SmiMI CAYNNNNRTG 3 cut(s) 241, 396, 720
SsiI CCGC 4 cut(s) 234, 361, 1048, 1370
SspMI CTAG 2 cut(s) 51, 1427
SstI GAGCTC 1 cut(s) 1275
StyD4I CCNGG 3 cut(s) 756, 916, 1110
StyI CCWWGG 1 cut(s) 500
TaaI ACNGT 2 cut(s) 154, 1366
TaiI ACGT 2 cut(s) 267, 990
TaqI TCGA 1 cut(s) 742
TaqII GACCGA 1 cut(s) 1083
TatI WGTACW 1 cut(s) 614
TfiI GAWTC 3 cut(s) 643, 725, 1382
Tru1I TTAA 6 cut(s) 219, 338, 387, 486, 762, 1292
Tru9I TTAA 6 cut(s) 219, 338, 387, 486, 762, 1292
TseI GCWGC 3 cut(s) 101, 545, 1224
TspDTI ATGAA 5 cut(s) 522, 738, 989, 993, 1090
TspGWI ACGGA 2 cut(s) 16, 247
VpaK11BI GGWCC 1 cut(s) 368
XapI RAATTY 4 cut(s) 45, 215, 248, 445
XceI RCATGY 1 cut(s) 982
XmiI GTMKAC 1 cut(s) 1211
XspI CTAG 2 cut(s) 51, 1427
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.