AT1G52760

Serine aminopeptidase, S33

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0009854
Physical Location & Seq
Forward (+)
19651172 .. 19652825
1654 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G52760.1

Sequence Viewer

Length: 999 bp
ATGCCGTCGGAAGCGGAGAGCTCAGCGAATTCAGCTCCGGCAACTCCGCCACCACCACCGAATTTCTGGGGAACCATGCCGGAGGAAGAGTACTACACTTCACAAGGAGTACGTAACAGCAAATCATACTTCGAAACACCAAACGGCAAGCTCTTCACTCAGAGCTTCTTACCATTAGATGGTGAAATCAAAGGCACTGTGTACATGTCTCATGGATACGGATCCGATACAAGCTGGATGTTTCAGAAGATCTGTATGAGTTTCTCTAGTTGGGGTTACGCTGTTTTCGCCGCCGATCTTCTCGGTCACGGCCGTTCCGATGGTATCCGCTGCTACATGGGTGATATGGAGAAAGTTGCAGCAACATCATTGGCTTTCTTCAAGCATGTTCGTTGTAGTGATCCATATAAGGATCTTCCGGCTTTTCTGTTTGGTGAATCGATGGGAGGTCTTGTGACGCTTTTGATGTATTTTCAATCGGAACCTGAGACTTGGACCGGTTTGATGTTTTCGGCTCCTCTCTTTGTTATCCCTGAGGATATGAAACCAAGCAAGGCTCATCTTTTTGCTTATGGTCTCCTCTTTGGTTTGGCTGATACGTGGGCTGCAATGCCGGATAATAAGATGGTTGGGAAGGCTATCAAGGACCCTGAAAAGCTTAAGATCATCGCTTCTAACCCGCAAAGATATACAGGGAAGCCTAGAGTGGGAACAATGAGAGAGTTACTGAGGAAGACTCAATACGTTCAGGAGAATTTCGGGAAAGTTACTATTCCGGTGTTTACGGCGCACGGGACAGCGGATGGAGTAACATGTCCTACATCTTCGAAGCTACTATACGAAAAAGCGTCAAGCGCTGATAAAACGTTGAAGATCTATGAAGGGATGTATCACTCGCTGATTCAAGGAGAGCCTGACGAGAACGCTGAGATAGTCTTGAAGGATATGAGAGAGTGGATCGATGAGAAGGTTAAGAAGTATGGATCTAAAACCGCTTGA

Protein Analysis

332

Amino Acids

36.97

Weight (kDa)

6.12

Isoelectric Point (pI)

29.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 62 - 305 1.6e-52 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 66 - 203 8.9e-08 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 70 - 308 1.8e-07 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009854)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G52760
fragaria_vesca FvH4_6g30610 FvH4_6g30610 FvH4_6g30610 FvH4_6g30610
malus_domestica MD17G1201900.v1.1
prunus_persica Prupe.5G109300_v2.0.a1
pyrus_communis pycom17g20580 pycom17g20590 pycom17g20600 pycom17g20610
rosa_chinensis RchiOBHm_Chr2g0137021
rosa_laevigata RLG00000019597
rosa_multiflora Rmu_sc0001374.1_g000047
rosa_roxburghii Rroxscaffold_2G00107770
rosa_rugosa Rorug02G0337200
rosa_samantha Rh2AG386300 Rh2BG393100 Rh2CG373600 Rh2DG409300
rosa_wichuraiana Rw2G031600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 179
AciI CCGC 7 cut(s) 14, 47, 291, 328, 680, 800, 993
AclI AACGTT 1 cut(s) 866
AclWI GGATC 6 cut(s) 216, 229, 395, 420, 965, 991
AcoI YGGCCR 1 cut(s) 310
AcsI RAATTY 3 cut(s) 28, 61, 754
AfaI GTAC 3 cut(s) 92, 111, 203
AfeI AGCGCT 1 cut(s) 856
AfiI CCNNNNNNNGG 2 cut(s) 179, 707
AflII CTTAAG 1 cut(s) 659
AflIII ACRYGT 2 cut(s) 204, 812
AgeI ACCGGT 1 cut(s) 497
AgsI TTSAA 5 cut(s) 382, 476, 871, 905, 940
AluBI AGCT 7 cut(s) 21, 35, 151, 165, 234, 658, 832
AluI AGCT 7 cut(s) 21, 35, 151, 165, 234, 658, 832
Alw21I GWGCWC 1 cut(s) 23
Alw26I GTCTC 3 cut(s) 213, 482, 581
AlwI GGATC 6 cut(s) 216, 229, 395, 420, 965, 991
Aor51HI AGCGCT 1 cut(s) 856
AoxI GGCC 1 cut(s) 310
ApeKI GCWGC 3 cut(s) 330, 359, 605
ApoI RAATTY 3 cut(s) 28, 61, 754
AsiGI ACCGGT 1 cut(s) 497
AspLEI GCGC 2 cut(s) 790, 857
AspS9I GGNCC 2 cut(s) 495, 646
AsuHPI GGTGA 3 cut(s) 194, 353, 446
AsuII TTCGAA 2 cut(s) 132, 827
AvaII GGWCC 2 cut(s) 495, 646
AxyI CCTNAGG 1 cut(s) 534
BamHI GGATCC 1 cut(s) 221
BanII GRGCYC 1 cut(s) 23
BarI GAAGNNNNNNTAC 4 cut(s) 725, 757, 821, 853
BbsI GAAGAC 1 cut(s) 740
Bbv12I GWGCWC 1 cut(s) 23
BbvI GCAGC 3 cut(s) 317, 371, 592
BccI CCATC 5 cut(s) 173, 314, 436, 619, 797
BceAI ACGGC 4 cut(s) 160, 297, 325, 801
BciVI GTATCC 2 cut(s) 209, 335
BcoDI GTCTC 3 cut(s) 213, 482, 581
BfaI CTAG 2 cut(s) 267, 702
BfoI RGCGCY 1 cut(s) 858
BfrI CTTAAG 1 cut(s) 659
BfuI GTATCC 2 cut(s) 209, 335
BglII AGATCT 2 cut(s) 249, 873
BisI GCNGC 4 cut(s) 291, 331, 360, 606
BlpI GCTNAGC 1 cut(s) 22
BlsI GCNGC 4 cut(s) 292, 332, 361, 607
BmcAI AGTACT 1 cut(s) 92
Bme18I GGWCC 2 cut(s) 495, 646
BmgT120I GGNCC 2 cut(s) 495, 646
BmiI GGNNCC 5 cut(s) 73, 223, 483, 516, 648
BpiI GAAGAC 1 cut(s) 740
BplI GAGNNNNNCTC 2 cut(s) 721, 753
Bpu1102I GCTNAGC 1 cut(s) 22
Bpu14I TTCGAA 2 cut(s) 132, 827
Bsa29I ATCGAT 2 cut(s) 440, 960
BsaAI YACGTR 2 cut(s) 113, 600
BsaBI GATNNNNATC 1 cut(s) 220
BsaI GGTCTC 1 cut(s) 581
BsaWI WCCGGW 2 cut(s) 497, 775
BsaXI ACNNNNNCTCC 4 cut(s) 74, 104, 438, 468
Bsc4I CCNNNNNNNGG 2 cut(s) 179, 707
Bse118I RCCGGY 1 cut(s) 497
Bse21I CCTNAGG 1 cut(s) 534
Bse3DI GCAATG 1 cut(s) 615
Bse8I GATNNNNATC 1 cut(s) 220
BseCI ATCGAT 2 cut(s) 440, 960
BseGI GGATG 3 cut(s) 243, 808, 891
BseJI GATNNNNATC 1 cut(s) 220
BseLI CCNNNNNNNGG 2 cut(s) 179, 707
BseMI GCAATG 1 cut(s) 615
BseMII CTCAG 6 cut(s) 36, 173, 477, 525, 719, 918
BseRI GAGGAG 2 cut(s) 507, 569
BseX3I CGGCCG 1 cut(s) 310
BseXI GCAGC 3 cut(s) 317, 371, 592
Bsh1285I CGRYCG 1 cut(s) 313
BshFI GGCC 1 cut(s) 312
BshTI ACCGGT 1 cut(s) 497
BshVI ATCGAT 2 cut(s) 440, 960
BsiEI CGRYCG 1 cut(s) 313
BsiHKAI GWGCWC 1 cut(s) 23
BsiSI CCGG 6 cut(s) 38, 80, 419, 498, 614, 776
BslFI GGGAC 1 cut(s) 808
BslI CCNNNNNNNGG 2 cut(s) 179, 707
BsmAI GTCTC 3 cut(s) 213, 482, 581
BsmFI GGGAC 1 cut(s) 808
BsnI GGCC 1 cut(s) 312
Bso31I GGTCTC 1 cut(s) 581
Bsp119I TTCGAA 2 cut(s) 132, 827
Bsp1286I GDGCHC 1 cut(s) 23
Bsp1407I TGTACA 1 cut(s) 201
Bsp143I GATC 9 cut(s) 221, 249, 295, 400, 412, 663, 873, 957, 983
Bsp1720I GCTNAGC 1 cut(s) 22
BspACI CCGC 7 cut(s) 14, 47, 291, 328, 680, 800, 993
BspANI GGCC 1 cut(s) 312
BspCNI CTCAG 6 cut(s) 35, 172, 478, 526, 720, 919
BspDI ATCGAT 2 cut(s) 440, 960
BspLI GGNNCC 5 cut(s) 73, 223, 483, 516, 648
BspPI GGATC 6 cut(s) 216, 229, 395, 420, 965, 991
BspQI GCTCTTC 1 cut(s) 158
BspT104I TTCGAA 2 cut(s) 132, 827
BspTI CTTAAG 1 cut(s) 659
BspTNI GGTCTC 1 cut(s) 581
BsrDI GCAATG 1 cut(s) 615
BsrFI RCCGGY 1 cut(s) 497
BsrGI TGTACA 1 cut(s) 201
BssAI RCCGGY 1 cut(s) 497
BssMI GATC 9 cut(s) 221, 249, 295, 400, 412, 663, 873, 957, 983
Bst4CI ACNGT 1 cut(s) 199
Bst6I CTCTTC 2 cut(s) 81, 158
BstAFI CTTAAG 1 cut(s) 659
BstAUI TGTACA 1 cut(s) 201
BstBAI YACGTR 2 cut(s) 113, 600
BstBI TTCGAA 2 cut(s) 132, 827
BstC8I GCNNGC 1 cut(s) 149
BstDEI CTNAG 6 cut(s) 22, 159, 486, 534, 728, 927
BstF5I GGATG 3 cut(s) 243, 808, 891
BstH2I RGCGCY 1 cut(s) 858
BstHHI GCGC 2 cut(s) 790, 857
BstKTI GATC 9 cut(s) 224, 252, 298, 403, 415, 666, 876, 960, 986
BstMAI GTCTC 3 cut(s) 213, 482, 581
BstMBI GATC 9 cut(s) 221, 249, 295, 400, 412, 663, 873, 957, 983
BstMCI CGRYCG 1 cut(s) 313
BstMWI GCNNNNNNNGC 3 cut(s) 32, 287, 854
BstNSI RCATGY 3 cut(s) 208, 389, 816
BstSNI TACGTA 1 cut(s) 113
BstV1I GCAGC 3 cut(s) 317, 371, 592
BstV2I GAAGAC 1 cut(s) 740
BstX2I RGATCY 5 cut(s) 221, 249, 412, 873, 983
BstYI RGATCY 5 cut(s) 221, 249, 412, 873, 983
BstZI CGGCCG 1 cut(s) 310
Bsu15I ATCGAT 2 cut(s) 440, 960
Bsu36I CCTNAGG 1 cut(s) 534
BsuI GTATCC 2 cut(s) 209, 335
BsuRI GGCC 1 cut(s) 312
BsuTUI ATCGAT 2 cut(s) 440, 960
BtgZI GCGATG 1 cut(s) 652
BtsCI GGATG 3 cut(s) 243, 808, 891
BtsIMutI CAGTG 1 cut(s) 195
Cac8I GCNNGC 1 cut(s) 149
CfoI GCGC 2 cut(s) 790, 857
Cfr10I RCCGGY 1 cut(s) 497
Cfr13I GGNCC 2 cut(s) 495, 646
ClaI ATCGAT 2 cut(s) 440, 960
CseI GACGC 2 cut(s) 466, 837
Csp6I GTAC 3 cut(s) 91, 110, 202
CspAI ACCGGT 1 cut(s) 497
CviAII CATG 6 cut(s) 76, 205, 212, 337, 386, 813
CviQI GTAC 3 cut(s) 91, 110, 202
DdeI CTNAG 6 cut(s) 22, 159, 486, 534, 728, 927
DpnI GATC 9 cut(s) 223, 251, 297, 402, 414, 665, 875, 959, 985
DpnII GATC 9 cut(s) 221, 249, 295, 400, 412, 663, 873, 957, 983
EaeI YGGCCR 1 cut(s) 310
EagI CGGCCG 1 cut(s) 310
Eam1104I CTCTTC 2 cut(s) 81, 158
EarI CTCTTC 2 cut(s) 81, 158
EciI GGCGGA 1 cut(s) 36
Ecl136II GAGCTC 1 cut(s) 21
EclXI CGGCCG 1 cut(s) 310
Eco105I TACGTA 1 cut(s) 113
Eco24I GRGCYC 1 cut(s) 23
Eco31I GGTCTC 1 cut(s) 581
Eco47I GGWCC 2 cut(s) 495, 646
Eco47III AGCGCT 1 cut(s) 856
Eco52I CGGCCG 1 cut(s) 310
Eco53kI GAGCTC 1 cut(s) 21
Eco81I CCTNAGG 1 cut(s) 534
EcoICRI GAGCTC 1 cut(s) 21
EcoO109I RGGNCCY 1 cut(s) 646
EcoRI GAATTC 1 cut(s) 28
EcoT38I GRGCYC 1 cut(s) 23
FaeI CATG 6 cut(s) 79, 208, 215, 340, 389, 816
FaqI GGGAC 1 cut(s) 808
FatI CATG 6 cut(s) 75, 204, 211, 336, 385, 812
FauI CCCGC 1 cut(s) 687
Fnu4HI GCNGC 4 cut(s) 291, 331, 360, 606
FokI GGATG 3 cut(s) 250, 815, 898
FriOI GRGCYC 1 cut(s) 23
Fsp4HI GCNGC 4 cut(s) 291, 331, 360, 606
FspBI CTAG 2 cut(s) 267, 702
GlaI GCGC 2 cut(s) 789, 856
GluI GCNGC 4 cut(s) 291, 331, 360, 606
HaeII RGCGCY 1 cut(s) 858
HaeIII GGCC 1 cut(s) 312
HapII CCGG 6 cut(s) 38, 80, 419, 498, 614, 776
HgaI GACGC 2 cut(s) 466, 837
HhaI GCGC 2 cut(s) 790, 857
Hin1II CATG 6 cut(s) 79, 208, 215, 340, 389, 816
Hin6I GCGC 2 cut(s) 788, 855
HinP1I GCGC 2 cut(s) 788, 855
HindIII AAGCTT 1 cut(s) 656
HinfI GANTC 3 cut(s) 437, 736, 901
HpaII CCGG 6 cut(s) 38, 80, 419, 498, 614, 776
HphI GGTGA 3 cut(s) 194, 353, 446
Hpy166II GTNNAC 2 cut(s) 202, 783
Hpy188I TCNGA 6 cut(s) 10, 162, 226, 246, 319, 481
Hpy188III TCNNGA 3 cut(s) 749, 760, 937
Hpy8I GTNNAC 2 cut(s) 202, 783
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 4 cut(s) 628, 875, 934, 961
HpyCH4III ACNGT 1 cut(s) 199
HpyCH4IV ACGT 4 cut(s) 112, 599, 744, 866
HpyCH4V TGCA 2 cut(s) 359, 608
HpyF10VI GCNNNNNNNGC 3 cut(s) 32, 287, 854
HpyF3I CTNAG 6 cut(s) 22, 159, 486, 534, 728, 927
HpySE526I ACGT 4 cut(s) 112, 599, 744, 866
Hsp92II CATG 6 cut(s) 79, 208, 215, 340, 389, 816
HspAI GCGC 2 cut(s) 788, 855
Kzo9I GATC 9 cut(s) 221, 249, 295, 400, 412, 663, 873, 957, 983
LguI GCTCTTC 1 cut(s) 158
LmnI GCTCC 2 cut(s) 40, 520
Lsp1109I GCAGC 3 cut(s) 317, 371, 592
MaeI CTAG 2 cut(s) 267, 702
MaeII ACGT 4 cut(s) 112, 599, 744, 866
MaeIII GTNAC 7 cut(s) 113, 275, 305, 454, 723, 766, 808
MalI GATC 9 cut(s) 223, 251, 297, 402, 414, 665, 875, 959, 985
MboI GATC 9 cut(s) 221, 249, 295, 400, 412, 663, 873, 957, 983
MboII GAAGA 9 cut(s) 98, 145, 259, 290, 370, 407, 745, 816, 883
MflI RGATCY 5 cut(s) 221, 249, 412, 873, 983
MhlI GDGCHC 1 cut(s) 23
MluCI AATT 3 cut(s) 28, 61, 754
MlyI GAGTC 1 cut(s) 730
MnlI CCTC 6 cut(s) 76, 440, 528, 529, 590, 723
MseI TTAA 2 cut(s) 660, 972
MspA1I CMGCKG 2 cut(s) 330, 800
MspCI CTTAAG 1 cut(s) 659
MspI CCGG 6 cut(s) 38, 80, 419, 498, 614, 776
MwoI GCNNNNNNNGC 3 cut(s) 32, 287, 854
NdeII GATC 9 cut(s) 221, 249, 295, 400, 412, 663, 873, 957, 983
NlaIII CATG 6 cut(s) 79, 208, 215, 340, 389, 816
NlaIV GGNNCC 5 cut(s) 73, 223, 483, 516, 648
NmuCI GTSAC 2 cut(s) 305, 454
NspI RCATGY 3 cut(s) 208, 389, 816
NspV TTCGAA 2 cut(s) 132, 827
PciI ACATGT 2 cut(s) 204, 812
PciSI GCTCTTC 1 cut(s) 158
PcsI WCGNNNNNNNCGW 1 cut(s) 315
PfeI GAWTC 2 cut(s) 437, 901
PflMI CCANNNNNTGG 1 cut(s) 179
PinAI ACCGGT 1 cut(s) 497
PkrI GCNGC 4 cut(s) 292, 332, 361, 607
PleI GAGTC 1 cut(s) 730
PpsI GAGTC 1 cut(s) 730
Ppu21I YACGTR 2 cut(s) 113, 600
PpuMI RGGWCCY 1 cut(s) 646
PscI ACATGT 2 cut(s) 204, 812
Psp124BI GAGCTC 1 cut(s) 23
Psp1406I AACGTT 1 cut(s) 866
Psp5II RGGWCCY 1 cut(s) 646
PspN4I GGNNCC 5 cut(s) 73, 223, 483, 516, 648
PspPI GGNCC 2 cut(s) 495, 646
PspPPI RGGWCCY 1 cut(s) 646
PsuI RGATCY 5 cut(s) 221, 249, 412, 873, 983
RsaI GTAC 3 cut(s) 92, 111, 203
RsaNI GTAC 3 cut(s) 91, 110, 202
SacI GAGCTC 1 cut(s) 23
SapI GCTCTTC 1 cut(s) 158
SaqAI TTAA 2 cut(s) 660, 972
SatI GCNGC 4 cut(s) 291, 331, 360, 606
Sau3AI GATC 9 cut(s) 221, 249, 295, 400, 412, 663, 873, 957, 983
Sau96I GGNCC 2 cut(s) 495, 646
ScaI AGTACT 1 cut(s) 92
SchI GAGTC 1 cut(s) 730
SduI GDGCHC 1 cut(s) 23
SfuI TTCGAA 2 cut(s) 132, 827
SinI GGWCC 2 cut(s) 495, 646
SmlI CTYRAG 1 cut(s) 659
SmoI CTYRAG 1 cut(s) 659
SnaBI TACGTA 1 cut(s) 113
Sse9I AATT 3 cut(s) 28, 61, 754
SsiI CCGC 7 cut(s) 14, 47, 291, 328, 680, 800, 993
SspMI CTAG 2 cut(s) 267, 702
SstI GAGCTC 1 cut(s) 23
TaaI ACNGT 1 cut(s) 199
TaiI ACGT 4 cut(s) 115, 602, 747, 869
TaqI TCGA 4 cut(s) 132, 440, 827, 960
TaqII GACCGA 1 cut(s) 293
TasI AATT 3 cut(s) 28, 61, 754
TatI WGTACW 2 cut(s) 90, 201
TauI GCSGC 1 cut(s) 293
TfiI GAWTC 2 cut(s) 437, 901
Tru1I TTAA 2 cut(s) 660, 972
Tru9I TTAA 2 cut(s) 660, 972
TscAI CASTG 1 cut(s) 202
TseFI GTSAC 2 cut(s) 305, 454
TseI GCWGC 3 cut(s) 330, 359, 605
Tsp45I GTSAC 2 cut(s) 305, 454
TspDTI ATGAA 2 cut(s) 557, 894
TspGWI ACGGA 1 cut(s) 234
TspRI CASTG 1 cut(s) 202
Van91I CCANNNNNTGG 1 cut(s) 179
Vha464I CTTAAG 1 cut(s) 659
VpaK11BI GGWCC 2 cut(s) 495, 646
XapI RAATTY 3 cut(s) 28, 61, 754
XceI RCATGY 3 cut(s) 208, 389, 816
XcmI CCANNNNNNNNNTGG 1 cut(s) 63
XspI CTAG 2 cut(s) 267, 702
ZrmI AGTACT 1 cut(s) 92
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.