Rh2CG373600

Serine aminopeptidase, S33

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0009854
Physical Location & Seq
Forward (+)
50689962 .. 50694388
4427 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG373600.1

Sequence Viewer

Length: 984 bp
ATGTCGACCGAAAAATCGGCACCGGCGCCGGAGACGCCTCCGAATTTCTGGGGCGACACGCCGGCGGAGGAGTACTACGCCTCCCAAGGGGTGCGCAACACCCAATCCTACTTCCCTACCCCCAACGGCCAGATCTTCACCCAGAGCTTCCTGCCGTTGGATCCCGACCTACCAGTCAAAGGCACCGTCTTCATGACCCACGGCTACGGCTCCGACACCGGCTGGCTCTTCCAGAAAATCTGCATCACCTTCTCCACCTGGGGCTACGCCGTCTTCGCCGCCGACCTCCTCGGCCACGGCCGCTCCGACGGCCTCCGCTGCTACCTCGGCGACATGGAGAAGGTCGCCGCCACCTCCCTCTCCTACTTCCTCCACGTCCGCCGCAGCGAGCCCTACCGCCGCCTCCCGGCCTTCCTTTTCGGCGAGTCGATGGGCGGCGCCGCCACCATGCTCATGTACTTCCAGTCCGACCCCGACACGTGGACGGGCCTGATCTTCTCGGCGCCGCTCTTCGTTATCCCGGAGAACATGAAGCCCAGCAAGCTTCACCTTTTCGTCTACGGGCTCCTGTTCGGCATCGCCGACACGTGGGCGGCGATGCCGGACAAGAAGATGGTCGGGAAGTCCATCAAGGATCCCGAGAAGCTGAAAATCATAGCGTCCAATCCGAGGAGGTACACTGGGGCCCCCAGGGTGGGGACCATGAGGGAGATTGCTAGGGTGTGCCAGTATATACAGGATAACTTCTCCCGCGTAACGGCGCCGTTTCTGACCGTGCATGGGACTGCCGACGGGGTGACGTGTCCGACGTCGTCGAAGCTGTTGTATGAGAAGGCATCGAGTGTGGACAAGAGCTTGAAGATTTACGATGGGATGTACCATTCCTTGATACAAGGAGAGACTGATGAGAATGCCGACCTTGTGTTGAAGGATATGAGGGAGTGGATCGATGAGAGGGCGGAGAAGTATGGACCTAAACCCTAA

Protein Analysis

327

Amino Acids

36.56

Weight (kDa)

6.32

Isoelectric Point (pI)

27.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 59 - 300 2.3e-50 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 62 - 198 7.3e-07 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 64 - 294 2.5e-06 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009854)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G52760
fragaria_vesca FvH4_6g30610 FvH4_6g30610 FvH4_6g30610 FvH4_6g30610
malus_domestica MD17G1201900.v1.1
prunus_persica Prupe.5G109300_v2.0.a1
pyrus_communis pycom17g20580 pycom17g20590 pycom17g20600 pycom17g20610
rosa_chinensis RchiOBHm_Chr2g0137021
rosa_laevigata RLG00000019597
rosa_multiflora Rmu_sc0001374.1_g000047
rosa_roxburghii Rroxscaffold_2G00107770
rosa_rugosa Rorug02G0337200
rosa_samantha Rh2AG386300 Rh2BG393100 Rh2CG373600 Rh2DG409300
rosa_wichuraiana Rw2G031600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 173
AatII GACGTC 1 cut(s) 812
Acc16I TGCGCA 1 cut(s) 95
AccB1I GGYRCC 6 cut(s) 19, 25, 182, 437, 502, 760
AccBSI CCGCTC 2 cut(s) 303, 508
AccI GTMKAC 2 cut(s) 5, 558
AccII CGCG 1 cut(s) 753
AclWI GGATC 5 cut(s) 155, 168, 629, 642, 953
AcoI YGGCCR 3 cut(s) 127, 292, 298
AcsI RAATTY 1 cut(s) 43
AcvI CACGTG 2 cut(s) 480, 588
AcyI GRCGYC 6 cut(s) 26, 35, 438, 503, 761, 809
AfaI GTAC 4 cut(s) 74, 458, 677, 878
AflIII ACRYGT 3 cut(s) 477, 585, 800
AgsI TTSAA 2 cut(s) 859, 928
AjiI CACGTC 2 cut(s) 376, 801
AjnI CCWGG 2 cut(s) 257, 689
AluBI AGCT 5 cut(s) 147, 544, 646, 820, 855
AluI AGCT 5 cut(s) 147, 544, 646, 820, 855
Alw26I GTCTC 2 cut(s) 26, 893
AlwI GGATC 5 cut(s) 155, 168, 629, 642, 953
Ama87I CYCGRG 1 cut(s) 638
AoxI GGCC 7 cut(s) 127, 292, 298, 310, 408, 487, 684
ApaI GGGCCC 1 cut(s) 688
ApeKI GCWGC 2 cut(s) 318, 384
ApoI RAATTY 1 cut(s) 43
ArsI GACNNNNNNTTYG 2 cut(s) 171, 203
AspLEI GCGC 5 cut(s) 28, 96, 440, 505, 763
AspS9I GGNCC 5 cut(s) 487, 684, 685, 699, 971
AsuC2I CCSGG 2 cut(s) 407, 521
AsuHPI GGTGA 4 cut(s) 130, 238, 539, 808
AvaI CYCGRG 1 cut(s) 638
AvaII GGWCC 2 cut(s) 699, 971
BaeGI GKGCMC 1 cut(s) 688
BamHI GGATCC 2 cut(s) 160, 634
BanI GGYRCC 6 cut(s) 19, 25, 182, 437, 502, 760
BanII GRGCYC 3 cut(s) 393, 567, 688
BbrPI CACGTG 2 cut(s) 480, 588
BbsI GAAGAC 2 cut(s) 181, 265
BbvI GCAGC 2 cut(s) 305, 396
BccI CCATC 4 cut(s) 424, 607, 635, 863
BceAI ACGGC 9 cut(s) 139, 142, 217, 223, 254, 313, 325, 748, 774
BciT130I CCWGG 2 cut(s) 259, 691
BcnI CCSGG 2 cut(s) 407, 521
BcoDI GTCTC 2 cut(s) 26, 893
BfaI CTAG 1 cut(s) 717
BfoI RGCGCY 4 cut(s) 29, 441, 506, 764
BglII AGATCT 1 cut(s) 132
BmcAI AGTACT 1 cut(s) 74
Bme1390I CCNGG 4 cut(s) 259, 407, 521, 691
Bme18I GGWCC 2 cut(s) 699, 971
BmeT110I CYCGRG 1 cut(s) 638
BmgBI CACGTC 2 cut(s) 376, 801
BmgT120I GGNCC 5 cut(s) 487, 684, 685, 699, 971
BmrFI CCNGG 4 cut(s) 259, 407, 521, 691
BmrI ACTGGG 1 cut(s) 690
BmsI GCATC 4 cut(s) 252, 585, 588, 845
BmuI ACTGGG 1 cut(s) 690
BpiI GAAGAC 2 cut(s) 181, 265
BpuMI CCSGG 2 cut(s) 407, 521
Bsa29I ATCGAT 1 cut(s) 948
BsaAI YACGTR 2 cut(s) 480, 588
BsaHI GRCGYC 6 cut(s) 26, 35, 438, 503, 761, 809
BsaJI CCNNGG 9 cut(s) 85, 199, 258, 289, 295, 325, 668, 689, 690
BsaXI ACNNNNNCTCC 4 cut(s) 65, 95, 287, 317
Bse118I RCCGGY 3 cut(s) 22, 61, 218
Bse1I ACTGG 4 cut(s) 173, 463, 685, 727
BseBI CCWGG 2 cut(s) 259, 691
BseCI ATCGAT 1 cut(s) 948
BseDI CCNNGG 9 cut(s) 85, 199, 258, 289, 295, 325, 668, 689, 690
BseGI GGATG 1 cut(s) 879
BseNI ACTGG 4 cut(s) 173, 463, 685, 727
BseRI GAGGAG 3 cut(s) 83, 278, 685
BseSI GKGCMC 1 cut(s) 688
BseX3I CGGCCG 1 cut(s) 298
BseXI GCAGC 2 cut(s) 305, 396
BseYI CCCAGC 1 cut(s) 536
Bsh1236I CGCG 1 cut(s) 753
Bsh1285I CGRYCG 2 cut(s) 9, 301
BshFI GGCC 7 cut(s) 129, 294, 300, 312, 410, 489, 686
BshNI GGYRCC 6 cut(s) 19, 25, 182, 437, 502, 760
BshVI ATCGAT 1 cut(s) 948
BsiEI CGRYCG 2 cut(s) 9, 301
BsiHKCI CYCGRG 1 cut(s) 638
BsiSI CCGG 7 cut(s) 23, 29, 62, 219, 407, 521, 602
BslFI GGGAC 2 cut(s) 712, 796
BsmAI GTCTC 2 cut(s) 26, 893
BsmBI CGTCTC 1 cut(s) 26
BsmFI GGGAC 2 cut(s) 712, 796
BsmI GAATGC 1 cut(s) 916
BsnI GGCC 7 cut(s) 129, 294, 300, 312, 410, 489, 686
BsoBI CYCGRG 1 cut(s) 638
Bsp120I GGGCCC 1 cut(s) 684
Bsp1286I GDGCHC 3 cut(s) 393, 567, 688
Bsp143I GATC 5 cut(s) 132, 160, 492, 634, 945
BspANI GGCC 7 cut(s) 129, 294, 300, 312, 410, 489, 686
BspDI ATCGAT 1 cut(s) 948
BspFNI CGCG 1 cut(s) 753
BspHI TCATGA 1 cut(s) 192
BspPI GGATC 5 cut(s) 155, 168, 629, 642, 953
BspQI GCTCTTC 2 cut(s) 233, 515
BspT107I GGYRCC 6 cut(s) 19, 25, 182, 437, 502, 760
BsrBI CCGCTC 2 cut(s) 303, 508
BsrFI RCCGGY 3 cut(s) 22, 61, 218
BsrI ACTGG 4 cut(s) 173, 463, 685, 727
BssAI RCCGGY 3 cut(s) 22, 61, 218
BssECI CCNNGG 9 cut(s) 85, 199, 258, 289, 295, 325, 668, 689, 690
BssMI GATC 5 cut(s) 132, 160, 492, 634, 945
BssNI GRCGYC 6 cut(s) 26, 35, 438, 503, 761, 809
BssT1I CCWWGG 1 cut(s) 85
Bst2UI CCWGG 2 cut(s) 259, 691
Bst4CI ACNGT 2 cut(s) 187, 775
Bst6I CTCTTC 2 cut(s) 233, 515
BstACI GRCGYC 6 cut(s) 26, 35, 438, 503, 761, 809
BstBAI YACGTR 2 cut(s) 480, 588
BstC8I GCNNGC 4 cut(s) 63, 224, 389, 542
BstDSI CCRYGG 2 cut(s) 199, 295
BstF5I GGATG 1 cut(s) 879
BstFNI CGCG 1 cut(s) 753
BstH2I RGCGCY 4 cut(s) 29, 441, 506, 764
BstHHI GCGC 5 cut(s) 28, 96, 440, 505, 763
BstKTI GATC 5 cut(s) 135, 163, 495, 637, 948
BstMAI GTCTC 2 cut(s) 26, 893
BstMBI GATC 5 cut(s) 132, 160, 492, 634, 945
BstMCI CGRYCG 2 cut(s) 9, 301
BstMWI GCNNNNNNNGC 7 cut(s) 34, 275, 300, 309, 318, 327, 541
BstNI CCWGG 2 cut(s) 259, 691
BstSCI CCNGG 4 cut(s) 257, 405, 519, 689
BstSLI GKGCMC 1 cut(s) 688
BstUI CGCG 1 cut(s) 753
BstV1I GCAGC 2 cut(s) 305, 396
BstV2I GAAGAC 2 cut(s) 181, 265
BstX2I RGATCY 3 cut(s) 132, 160, 634
BstYI RGATCY 3 cut(s) 132, 160, 634
BstZI CGGCCG 1 cut(s) 298
Bsu15I ATCGAT 1 cut(s) 948
BsuRI GGCC 7 cut(s) 129, 294, 300, 312, 410, 489, 686
BsuTUI ATCGAT 1 cut(s) 948
BtgI CCRYGG 2 cut(s) 199, 295
BtgZI GCGATG 2 cut(s) 562, 611
BtrI CACGTC 2 cut(s) 376, 801
BtsCI GGATG 1 cut(s) 879
BtsIMutI CAGTG 1 cut(s) 678
Cac8I GCNNGC 4 cut(s) 63, 224, 389, 542
CciI TCATGA 1 cut(s) 192
CfoI GCGC 5 cut(s) 28, 96, 440, 505, 763
Cfr10I RCCGGY 3 cut(s) 22, 61, 218
Cfr13I GGNCC 5 cut(s) 487, 684, 685, 699, 971
ClaI ATCGAT 1 cut(s) 948
CseI GACGC 2 cut(s) 43, 648
Csp6I GTAC 4 cut(s) 73, 457, 676, 877
CviAII CATG 7 cut(s) 193, 334, 448, 454, 529, 703, 779
CviQI GTAC 4 cut(s) 73, 457, 676, 877
DinI GGCGCC 4 cut(s) 27, 439, 504, 762
DpnI GATC 5 cut(s) 134, 162, 494, 636, 947
DpnII GATC 5 cut(s) 132, 160, 492, 634, 945
DrdI GACNNNNNNGTC 1 cut(s) 173
DseDI GACNNNNNNGTC 1 cut(s) 173
EaeI YGGCCR 3 cut(s) 127, 292, 298
EagI CGGCCG 1 cut(s) 298
Eam1104I CTCTTC 2 cut(s) 233, 515
EarI CTCTTC 2 cut(s) 233, 515
EciI GGCGGA 3 cut(s) 80, 368, 974
EclXI CGGCCG 1 cut(s) 298
Eco130I CCWWGG 1 cut(s) 85
Eco24I GRGCYC 3 cut(s) 393, 567, 688
Eco47I GGWCC 2 cut(s) 699, 971
Eco52I CGGCCG 1 cut(s) 298
Eco72I CACGTG 2 cut(s) 480, 588
Eco88I CYCGRG 1 cut(s) 638
EcoO109I RGGNCCY 2 cut(s) 684, 685
EcoRII CCWGG 2 cut(s) 257, 689
EcoT14I CCWWGG 1 cut(s) 85
EcoT38I GRGCYC 3 cut(s) 393, 567, 688
EgeI GGCGCC 4 cut(s) 27, 439, 504, 762
EheI GGCGCC 4 cut(s) 27, 439, 504, 762
ErhI CCWWGG 1 cut(s) 85
Esp3I CGTCTC 1 cut(s) 26
FaeI CATG 7 cut(s) 196, 337, 451, 457, 532, 706, 782
FaqI GGGAC 2 cut(s) 712, 796
FatI CATG 7 cut(s) 192, 333, 447, 453, 528, 702, 778
FauI CCCGC 1 cut(s) 758
FblI GTMKAC 2 cut(s) 5, 558
FokI GGATG 1 cut(s) 886
FriOI GRGCYC 3 cut(s) 393, 567, 688
FspBI CTAG 1 cut(s) 717
FspI TGCGCA 1 cut(s) 95
GlaI GCGC 5 cut(s) 27, 95, 439, 504, 762
GsaI CCCAGC 1 cut(s) 540
HaeII RGCGCY 4 cut(s) 29, 441, 506, 764
HaeIII GGCC 7 cut(s) 129, 294, 300, 312, 410, 489, 686
HapII CCGG 7 cut(s) 23, 29, 62, 219, 407, 521, 602
HgaI GACGC 2 cut(s) 43, 648
HhaI GCGC 5 cut(s) 28, 96, 440, 505, 763
Hin1I GRCGYC 6 cut(s) 26, 35, 438, 503, 761, 809
Hin1II CATG 7 cut(s) 196, 337, 451, 457, 532, 706, 782
Hin6I GCGC 5 cut(s) 26, 94, 438, 503, 761
HinP1I GCGC 5 cut(s) 26, 94, 438, 503, 761
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HindIII AAGCTT 1 cut(s) 542
HinfI GANTC 1 cut(s) 425
HpaII CCGG 7 cut(s) 23, 29, 62, 219, 407, 521, 602
HphI GGTGA 4 cut(s) 130, 238, 539, 808
Hpy166II GTNNAC 5 cut(s) 6, 483, 559, 678, 847
Hpy188I TCNGA 7 cut(s) 42, 214, 307, 469, 669, 771, 807
Hpy188III TCNNGA 5 cut(s) 164, 193, 232, 619, 638
Hpy8I GTNNAC 5 cut(s) 6, 483, 559, 678, 847
Hpy99I CGWCG 5 cut(s) 311, 794, 811, 814, 817
HpyAV CCTTC 5 cut(s) 259, 334, 421, 826, 922
HpyCH4III ACNGT 2 cut(s) 187, 775
HpyCH4IV ACGT 5 cut(s) 375, 479, 587, 800, 809
HpyCH4V TGCA 2 cut(s) 243, 778
HpyF10VI GCNNNNNNNGC 7 cut(s) 34, 275, 300, 309, 318, 327, 541
HpySE526I ACGT 5 cut(s) 375, 479, 587, 800, 809
Hsp92I GRCGYC 6 cut(s) 26, 35, 438, 503, 761, 809
Hsp92II CATG 7 cut(s) 196, 337, 451, 457, 532, 706, 782
HspAI GCGC 5 cut(s) 26, 94, 438, 503, 761
KasI GGCGCC 4 cut(s) 25, 437, 502, 760
KroI GCCGGC 1 cut(s) 61
KroNI GCCGGC 1 cut(s) 63
Kzo9I GATC 5 cut(s) 132, 160, 492, 634, 945
LguI GCTCTTC 2 cut(s) 233, 515
LmnI GCTCC 3 cut(s) 215, 308, 570
Lsp1109I GCAGC 2 cut(s) 305, 396
LweI GCATC 4 cut(s) 252, 585, 588, 845
MaeI CTAG 1 cut(s) 717
MaeII ACGT 5 cut(s) 375, 479, 587, 800, 809
MaeIII GTNAC 2 cut(s) 754, 796
MalI GATC 5 cut(s) 134, 162, 494, 636, 947
MbiI CCGCTC 2 cut(s) 303, 508
MboI GATC 5 cut(s) 132, 160, 492, 634, 945
MboII GAAGA 8 cut(s) 127, 181, 220, 265, 487, 502, 622, 871
MflI RGATCY 3 cut(s) 132, 160, 634
MhlI GDGCHC 3 cut(s) 393, 567, 688
MluCI AATT 1 cut(s) 43
Mly113I GGCGCC 4 cut(s) 26, 438, 503, 761
MlyI GAGTC 1 cut(s) 434
MmeI TCCRAC 5 cut(s) 138, 237, 330, 492, 830
MreI CGCCGGCG 1 cut(s) 61
MroNI GCCGGC 1 cut(s) 61
MslI CAYNNNNRTG 1 cut(s) 452
MspA1I CMGCKG 1 cut(s) 318
MspI CCGG 7 cut(s) 23, 29, 62, 219, 407, 521, 602
MspR9I CCNGG 4 cut(s) 259, 407, 521, 691
Mva1269I GAATGC 1 cut(s) 916
MvaI CCWGG 2 cut(s) 259, 691
MvnI CGCG 1 cut(s) 753
MwoI GCNNNNNNNGC 7 cut(s) 34, 275, 300, 309, 318, 327, 541
NaeI GCCGGC 1 cut(s) 63
NarI GGCGCC 4 cut(s) 26, 438, 503, 761
NciI CCSGG 2 cut(s) 407, 521
NdeII GATC 5 cut(s) 132, 160, 492, 634, 945
NgoMIV GCCGGC 1 cut(s) 61
NlaIII CATG 7 cut(s) 196, 337, 451, 457, 532, 706, 782
NmeAIII GCCGAG 3 cut(s) 270, 306, 479
NmuCI GTSAC 1 cut(s) 796
NsbI TGCGCA 1 cut(s) 95
PagI TCATGA 1 cut(s) 192
PasI CCCWGGG 1 cut(s) 690
PciSI GCTCTTC 2 cut(s) 233, 515
PcsI WCGNNNNNNNCGW 4 cut(s) 303, 579, 593, 806
PctI GAATGC 1 cut(s) 916
PdiI GCCGGC 1 cut(s) 63
PflFI GACNNNGTC 1 cut(s) 811
PfoI TCCNGGA 1 cut(s) 519
PleI GAGTC 1 cut(s) 433
PluTI GGCGCC 4 cut(s) 29, 441, 506, 764
PmaCI CACGTG 2 cut(s) 480, 588
PmlI CACGTG 2 cut(s) 480, 588
PpsI GAGTC 1 cut(s) 433
Ppu21I YACGTR 2 cut(s) 480, 588
Psp6I CCWGG 2 cut(s) 257, 689
PspCI CACGTG 2 cut(s) 480, 588
PspFI CCCAGC 1 cut(s) 536
PspGI CCWGG 2 cut(s) 257, 689
PspOMI GGGCCC 1 cut(s) 684
PspPI GGNCC 5 cut(s) 487, 684, 685, 699, 971
PsuI RGATCY 3 cut(s) 132, 160, 634
PsyI GACNNNGTC 1 cut(s) 811
RsaI GTAC 4 cut(s) 74, 458, 677, 878
RsaNI GTAC 4 cut(s) 73, 457, 676, 877
RseI CAYNNNNRTG 1 cut(s) 452
SalI GTCGAC 1 cut(s) 4
SapI GCTCTTC 2 cut(s) 233, 515
Sau3AI GATC 5 cut(s) 132, 160, 492, 634, 945
Sau96I GGNCC 5 cut(s) 487, 684, 685, 699, 971
ScaI AGTACT 1 cut(s) 74
SchI GAGTC 1 cut(s) 434
ScrFI CCNGG 4 cut(s) 259, 407, 521, 691
SduI GDGCHC 3 cut(s) 393, 567, 688
SfaNI GCATC 4 cut(s) 252, 585, 588, 845
SfoI GGCGCC 4 cut(s) 27, 439, 504, 762
SgrAI CRCCGGYG 2 cut(s) 22, 61
SinI GGWCC 2 cut(s) 699, 971
SmiMI CAYNNNNRTG 1 cut(s) 452
Sse9I AATT 1 cut(s) 43
SspDI GGCGCC 4 cut(s) 25, 437, 502, 760
SspMI CTAG 1 cut(s) 717
StyD4I CCNGG 4 cut(s) 257, 405, 519, 689
StyI CCWWGG 1 cut(s) 85
TaaI ACNGT 2 cut(s) 187, 775
TaiI ACGT 5 cut(s) 378, 482, 590, 803, 812
TaqI TCGA 5 cut(s) 5, 428, 815, 839, 948
TaqII GACCGA 1 cut(s) 23
TasI AATT 1 cut(s) 43
TatI WGTACW 2 cut(s) 72, 456
TauI GCSGC 9 cut(s) 281, 303, 350, 384, 402, 438, 443, 508, 596
TscAI CASTG 1 cut(s) 685
TseFI GTSAC 1 cut(s) 796
TseI GCWGC 2 cut(s) 318, 384
Tsp45I GTSAC 1 cut(s) 796
TspDTI ATGAA 2 cut(s) 181, 545
TspRI CASTG 1 cut(s) 685
Tth111I GACNNNGTC 1 cut(s) 811
VpaK11BI GGWCC 2 cut(s) 699, 971
XapI RAATTY 1 cut(s) 43
XmiI GTMKAC 2 cut(s) 5, 558
XspI CTAG 1 cut(s) 717
ZraI GACGTC 1 cut(s) 810
ZrmI AGTACT 1 cut(s) 74
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.