pycom17g20580

Serine aminopeptidase, S33

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0009854
Physical Location & Seq
Reverse (-)
19002513 .. 19003310
798 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g20580.1

Sequence Viewer

Length: 798 bp
ATGTCGACCGAGACGCCACCGAATTTCTGGGGCGACATGCTGGAGGAGGAGTACTACACCTCCCAAGGAGTGCGCAACACCAAATCCTTCTTCCAAACCTCCAACGGCCAGATCTTCACCCAGTCCTTCCTGCCCTTGGATCAAAAAGTCAAAGCCACCGTCTTCATGACCCACGGCTACGGATCCGACACCGGCTGGCTCTTCCAGAAAATCTGCATCCACTACGCCACCTGGGGCTACGCCATCTTCGCCGCCGACCTCCTCGGCCACGGCCGGTCCGATGGCATCCGCTGCTACCTCGGCGACATGGAAAAAGTCGCCGCCAGCTCCCTCTCCTACTTCCTCCACGTCCGCCAGTCCAAACCCTACGTCTGCCTCCCGGCCTTCCTGTTCGGCGAGTCCCTGGGCGGCTTAGCCACCATGCTCATGTACTTCCAATCCCCTCCCGATACGTGGACAGGCTTGATCTTCTCCGCCCAGCAAAGTCCATCTCTTCATGTACGGCCTCCTCTTCGGAATCGCCGACATGGGATGTGCAAGTACGTGCAGTACAATTTCTCACGAGTGACGGCGCCGTTTTTGACAGTGCACGGGACGTTGGACGGGGTGACGTGTCCGACGTCGTCGCAGCTGCTGTACGAGAAGGGATCGAGTGTGGACAAGACTTTGAAGATGTACGATGGGATGTACCATTCGTTGATACAAGGGGAGGAGGACGAGAACGCTGAGATTGTATTGAGGGATATGAGAGAGTGGATTGACGAAAGAGTTGAGAGGAATGGTGGGCCCAATATTTGA

Protein Analysis

266

Amino Acids

30.06

Weight (kDa)

6.06

Isoelectric Point (pI)

46.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 48 - 159 9.8e-20 Serine aminopeptidase, S33
Hydrolase_4 PF12146 178 - 236 3.1e-11 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0009854)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G52760
fragaria_vesca FvH4_6g30610 FvH4_6g30610 FvH4_6g30610 FvH4_6g30610
malus_domestica MD17G1201900.v1.1
prunus_persica Prupe.5G109300_v2.0.a1
pyrus_communis pycom17g20580 pycom17g20590 pycom17g20600 pycom17g20610
rosa_chinensis RchiOBHm_Chr2g0137021
rosa_laevigata RLG00000019597
rosa_multiflora Rmu_sc0001374.1_g000047
rosa_roxburghii Rroxscaffold_2G00107770
rosa_rugosa Rorug02G0337200
rosa_samantha Rh2AG386300 Rh2BG393100 Rh2CG373600 Rh2DG409300
rosa_wichuraiana Rw2G031600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 623
Acc16I TGCGCA 1 cut(s) 74
AccB1I GGYRCC 1 cut(s) 571
AccI GTMKAC 1 cut(s) 5
AciI CCGC 6 cut(s) 252, 289, 321, 352, 408, 474
AclWI GGATC 4 cut(s) 147, 177, 190, 655
AcoI YGGCCR 3 cut(s) 106, 265, 271
AcsI RAATTY 1 cut(s) 22
AcyI GRCGYC 3 cut(s) 14, 572, 620
AfaI GTAC 8 cut(s) 53, 431, 501, 542, 551, 638, 677, 689
AfiI CCNNNNNNNGG 2 cut(s) 136, 453
AflIII ACRYGT 1 cut(s) 611
AgsI TTSAA 1 cut(s) 670
AjiI CACGTC 2 cut(s) 349, 612
AjnI CCWGG 2 cut(s) 230, 402
AjuI GAANNNNNNNTTGG 2 cut(s) 74, 106
AluBI AGCT 2 cut(s) 327, 631
AluI AGCT 2 cut(s) 327, 631
Alw21I GWGCWC 1 cut(s) 591
Alw26I GTCTC 1 cut(s) 5
Alw44I GTGCAC 1 cut(s) 587
AlwI GGATC 4 cut(s) 147, 177, 190, 655
AlwNI CAGNNNCTG 1 cut(s) 634
AoxI GGCC 6 cut(s) 106, 265, 271, 381, 503, 785
ApaI GGGCCC 1 cut(s) 789
ApaLI GTGCAC 1 cut(s) 587
ApeKI GCWGC 3 cut(s) 291, 628, 631
ApoI RAATTY 1 cut(s) 22
ArsI GACNNNNNNTTYG 4 cut(s) 144, 176, 354, 386
AspLEI GCGC 2 cut(s) 75, 574
AspS9I GGNCC 3 cut(s) 276, 785, 786
AsuC2I CCSGG 1 cut(s) 380
AsuHPI GGTGA 2 cut(s) 109, 619
AvaII GGWCC 1 cut(s) 276
BaeGI GKGCMC 2 cut(s) 591, 789
BamHI GGATCC 1 cut(s) 182
BanI GGYRCC 1 cut(s) 571
BanII GRGCYC 1 cut(s) 789
BauI CACGAG 1 cut(s) 561
BbsI GAAGAC 1 cut(s) 154
Bbv12I GWGCWC 1 cut(s) 591
BbvI GCAGC 3 cut(s) 278, 618, 640
BccI CCATC 4 cut(s) 251, 275, 496, 674
BceAI ACGGC 6 cut(s) 121, 190, 286, 518, 559, 585
BciT130I CCWGG 2 cut(s) 232, 404
BcnI CCSGG 1 cut(s) 380
BcoDI GTCTC 1 cut(s) 5
BfoI RGCGCY 1 cut(s) 575
BglII AGATCT 1 cut(s) 111
BisI GCNGC 6 cut(s) 252, 292, 321, 409, 629, 632
BlpI GCTNAGC 1 cut(s) 412
BlsI GCNGC 6 cut(s) 253, 293, 322, 410, 630, 633
BmcAI AGTACT 1 cut(s) 53
Bme1390I CCNGG 3 cut(s) 232, 380, 404
Bme18I GGWCC 1 cut(s) 276
BmgBI CACGTC 2 cut(s) 349, 612
BmgT120I GGNCC 3 cut(s) 276, 785, 786
BmiI GGNNCC 3 cut(s) 184, 573, 787
BmrFI CCNGG 3 cut(s) 232, 380, 404
BmrI ACTGGG 1 cut(s) 115
BmsI GCATC 2 cut(s) 225, 294
BmuI ACTGGG 1 cut(s) 115
BpiI GAAGAC 1 cut(s) 154
BpmI CTGGAG 1 cut(s) 62
Bpu1102I GCTNAGC 1 cut(s) 412
BpuMI CCSGG 1 cut(s) 380
BsaAI YACGTR 2 cut(s) 453, 544
BsaHI GRCGYC 3 cut(s) 14, 572, 620
BsaJI CCNNGG 9 cut(s) 64, 135, 172, 231, 262, 268, 298, 402, 403
BsaXI ACNNNNNCTCC 4 cut(s) 35, 44, 65, 74
Bsc4I CCNNNNNNNGG 2 cut(s) 136, 453
Bse118I RCCGGY 2 cut(s) 191, 273
Bse1I ACTGG 2 cut(s) 121, 355
BseBI CCWGG 2 cut(s) 232, 404
BseDI CCNNGG 9 cut(s) 64, 135, 172, 231, 262, 268, 298, 402, 403
BseGI GGATG 4 cut(s) 216, 285, 537, 690
BseLI CCNNNNNNNGG 2 cut(s) 136, 453
BseMII CTCAG 1 cut(s) 717
BseNI ACTGG 2 cut(s) 121, 355
BseRI GAGGAG 5 cut(s) 59, 62, 251, 498, 725
BseSI GKGCMC 2 cut(s) 591, 789
BseX3I CGGCCG 1 cut(s) 271
BseXI GCAGC 3 cut(s) 278, 618, 640
BseYI CCCAGC 1 cut(s) 477
BsgI GTGCAG 1 cut(s) 566
Bsh1285I CGRYCG 2 cut(s) 9, 274
BshFI GGCC 6 cut(s) 108, 267, 273, 383, 505, 787
BshNI GGYRCC 1 cut(s) 571
BsiEI CGRYCG 2 cut(s) 9, 274
BsiHKAI GWGCWC 1 cut(s) 591
BsiSI CCGG 3 cut(s) 192, 274, 380
BslFI GGGAC 2 cut(s) 385, 607
BslI CCNNNNNNNGG 2 cut(s) 136, 453
BsmAI GTCTC 1 cut(s) 5
BsmBI CGTCTC 1 cut(s) 5
BsmFI GGGAC 2 cut(s) 385, 607
BsnI GGCC 6 cut(s) 108, 267, 273, 383, 505, 787
Bsp120I GGGCCC 1 cut(s) 785
Bsp1286I GDGCHC 2 cut(s) 591, 789
Bsp143I GATC 5 cut(s) 111, 139, 182, 465, 647
Bsp1720I GCTNAGC 1 cut(s) 412
BspACI CCGC 6 cut(s) 252, 289, 321, 352, 408, 474
BspANI GGCC 6 cut(s) 108, 267, 273, 383, 505, 787
BspCNI CTCAG 1 cut(s) 718
BspHI TCATGA 1 cut(s) 165
BspLI GGNNCC 3 cut(s) 184, 573, 787
BspPI GGATC 4 cut(s) 147, 177, 190, 655
BspQI GCTCTTC 1 cut(s) 206
BspT107I GGYRCC 1 cut(s) 571
BsrFI RCCGGY 2 cut(s) 191, 273
BsrI ACTGG 2 cut(s) 121, 355
BssAI RCCGGY 2 cut(s) 191, 273
BssECI CCNNGG 9 cut(s) 64, 135, 172, 231, 262, 268, 298, 402, 403
BssMI GATC 5 cut(s) 111, 139, 182, 465, 647
BssNI GRCGYC 3 cut(s) 14, 572, 620
BssSI CACGAG 1 cut(s) 561
BssT1I CCWWGG 2 cut(s) 64, 135
Bst2BI CACGAG 1 cut(s) 561
Bst2UI CCWGG 2 cut(s) 232, 404
Bst4CI ACNGT 2 cut(s) 160, 586
Bst6I CTCTTC 3 cut(s) 206, 498, 516
BstACI GRCGYC 3 cut(s) 14, 572, 620
BstAPI GCANNNNNTGC 1 cut(s) 291
BstBAI YACGTR 2 cut(s) 453, 544
BstC8I GCNNGC 2 cut(s) 197, 325
BstDEI CTNAG 2 cut(s) 412, 726
BstDSI CCRYGG 2 cut(s) 172, 268
BstF5I GGATG 4 cut(s) 216, 285, 537, 690
BstH2I RGCGCY 1 cut(s) 575
BstHHI GCGC 2 cut(s) 75, 574
BstKTI GATC 5 cut(s) 114, 142, 185, 468, 650
BstMAI GTCTC 1 cut(s) 5
BstMBI GATC 5 cut(s) 111, 139, 182, 465, 647
BstMCI CGRYCG 2 cut(s) 9, 274
BstMWI GCNNNNNNNGC 3 cut(s) 248, 291, 300
BstNI CCWGG 2 cut(s) 232, 404
BstNSI RCATGY 1 cut(s) 40
BstSCI CCNGG 3 cut(s) 230, 378, 402
BstSLI GKGCMC 2 cut(s) 591, 789
BstV1I GCAGC 3 cut(s) 278, 618, 640
BstV2I GAAGAC 1 cut(s) 154
BstX2I RGATCY 2 cut(s) 111, 182
BstYI RGATCY 2 cut(s) 111, 182
BstZI CGGCCG 1 cut(s) 271
BsuRI GGCC 6 cut(s) 108, 267, 273, 383, 505, 787
BtgI CCRYGG 2 cut(s) 172, 268
BtrI CACGTC 2 cut(s) 349, 612
BtsCI GGATG 4 cut(s) 216, 285, 537, 690
BtsIMutI CAGTG 1 cut(s) 591
Cac8I GCNNGC 2 cut(s) 197, 325
CaiI CAGNNNCTG 1 cut(s) 634
CciI TCATGA 1 cut(s) 165
CfoI GCGC 2 cut(s) 75, 574
Cfr10I RCCGGY 2 cut(s) 191, 273
Cfr13I GGNCC 3 cut(s) 276, 785, 786
CpoI CGGWCCG 1 cut(s) 276
CseI GACGC 1 cut(s) 22
Csp6I GTAC 8 cut(s) 52, 430, 500, 541, 550, 637, 676, 688
CspI CGGWCCG 1 cut(s) 276
CviAII CATG 7 cut(s) 37, 166, 307, 421, 427, 497, 527
CviQI GTAC 8 cut(s) 52, 430, 500, 541, 550, 637, 676, 688
DdeI CTNAG 2 cut(s) 412, 726
DinI GGCGCC 1 cut(s) 573
DpnI GATC 5 cut(s) 113, 141, 184, 467, 649
DpnII GATC 5 cut(s) 111, 139, 182, 465, 647
EaeI YGGCCR 3 cut(s) 106, 265, 271
EagI CGGCCG 1 cut(s) 271
Eam1104I CTCTTC 3 cut(s) 206, 498, 516
EarI CTCTTC 3 cut(s) 206, 498, 516
EciI GGCGGA 2 cut(s) 341, 463
EclXI CGGCCG 1 cut(s) 271
Eco130I CCWWGG 2 cut(s) 64, 135
Eco24I GRGCYC 1 cut(s) 789
Eco47I GGWCC 1 cut(s) 276
Eco52I CGGCCG 1 cut(s) 271
EcoRII CCWGG 2 cut(s) 230, 402
EcoT14I CCWWGG 2 cut(s) 64, 135
EcoT38I GRGCYC 1 cut(s) 789
EgeI GGCGCC 1 cut(s) 573
EheI GGCGCC 1 cut(s) 573
ErhI CCWWGG 2 cut(s) 64, 135
Esp3I CGTCTC 1 cut(s) 5
FaeI CATG 7 cut(s) 40, 169, 310, 424, 430, 500, 530
FaiI YATR 8 cut(s) 38, 167, 308, 422, 428, 498, 528, 746
FaqI GGGAC 2 cut(s) 385, 607
FatI CATG 7 cut(s) 36, 165, 306, 420, 426, 496, 526
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 6 cut(s) 252, 292, 321, 409, 629, 632
FokI GGATG 4 cut(s) 203, 272, 544, 697
FriOI GRGCYC 1 cut(s) 789
Fsp4HI GCNGC 6 cut(s) 252, 292, 321, 409, 629, 632
FspI TGCGCA 1 cut(s) 74
GlaI GCGC 2 cut(s) 74, 573
GluI GCNGC 6 cut(s) 252, 292, 321, 409, 629, 632
GsaI CCCAGC 1 cut(s) 481
GsuI CTGGAG 1 cut(s) 62
HaeII RGCGCY 1 cut(s) 575
HaeIII GGCC 6 cut(s) 108, 267, 273, 383, 505, 787
HapII CCGG 3 cut(s) 192, 274, 380
HgaI GACGC 1 cut(s) 22
HhaI GCGC 2 cut(s) 75, 574
Hin1I GRCGYC 3 cut(s) 14, 572, 620
Hin1II CATG 7 cut(s) 40, 169, 310, 424, 430, 500, 530
Hin6I GCGC 2 cut(s) 73, 572
HinP1I GCGC 2 cut(s) 73, 572
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HinfI GANTC 2 cut(s) 398, 517
HpaII CCGG 3 cut(s) 192, 274, 380
HphI GGTGA 2 cut(s) 109, 619
Hpy166II GTNNAC 4 cut(s) 6, 456, 589, 658
Hpy188I TCNGA 4 cut(s) 187, 280, 516, 618
Hpy188III TCNNGA 4 cut(s) 166, 205, 446, 561
Hpy8I GTNNAC 4 cut(s) 6, 456, 589, 658
Hpy99I CGWCG 3 cut(s) 622, 625, 628
HpyAV CCTTC 4 cut(s) 97, 136, 394, 637
HpyCH4III ACNGT 2 cut(s) 160, 586
HpyCH4IV ACGT 7 cut(s) 348, 369, 452, 543, 596, 611, 620
HpyCH4V TGCA 4 cut(s) 216, 537, 547, 589
HpyF10VI GCNNNNNNNGC 3 cut(s) 248, 291, 300
HpyF3I CTNAG 2 cut(s) 412, 726
HpySE526I ACGT 7 cut(s) 348, 369, 452, 543, 596, 611, 620
Hsp92I GRCGYC 3 cut(s) 14, 572, 620
Hsp92II CATG 7 cut(s) 40, 169, 310, 424, 430, 500, 530
HspAI GCGC 2 cut(s) 73, 572
KasI GGCGCC 1 cut(s) 571
Kzo9I GATC 5 cut(s) 111, 139, 182, 465, 647
LguI GCTCTTC 1 cut(s) 206
LmnI GCTCC 1 cut(s) 332
Lsp1109I GCAGC 3 cut(s) 278, 618, 640
LweI GCATC 2 cut(s) 225, 294
MaeII ACGT 7 cut(s) 348, 369, 452, 543, 596, 611, 620
MaeIII GTNAC 2 cut(s) 565, 607
MalI GATC 5 cut(s) 113, 141, 184, 467, 649
MboI GATC 5 cut(s) 111, 139, 182, 465, 647
MboII GAAGA 9 cut(s) 82, 106, 154, 193, 238, 460, 485, 503, 682
MflI RGATCY 2 cut(s) 111, 182
MhlI GDGCHC 2 cut(s) 591, 789
MluCI AATT 2 cut(s) 22, 553
Mly113I GGCGCC 1 cut(s) 572
MlyI GAGTC 1 cut(s) 407
MmeI TCCRAC 4 cut(s) 126, 210, 579, 641
MslI CAYNNNNRTG 1 cut(s) 425
MspA1I CMGCKG 2 cut(s) 291, 631
MspI CCGG 3 cut(s) 192, 274, 380
MspR9I CCNGG 3 cut(s) 232, 380, 404
MvaI CCWGG 2 cut(s) 232, 404
MwoI GCNNNNNNNGC 3 cut(s) 248, 291, 300
NarI GGCGCC 1 cut(s) 572
NciI CCSGG 1 cut(s) 380
NdeII GATC 5 cut(s) 111, 139, 182, 465, 647
NlaIII CATG 7 cut(s) 40, 169, 310, 424, 430, 500, 530
NlaIV GGNNCC 3 cut(s) 184, 573, 787
NmeAIII GCCGAG 2 cut(s) 243, 279
NmuCI GTSAC 2 cut(s) 565, 607
NsbI TGCGCA 1 cut(s) 74
NspI RCATGY 1 cut(s) 40
PagI TCATGA 1 cut(s) 165
PasI CCCWGGG 1 cut(s) 403
PciSI GCTCTTC 1 cut(s) 206
PcsI WCGNNNNNNNCGW 3 cut(s) 276, 520, 617
PfeI GAWTC 1 cut(s) 517
PflFI GACNNNGTC 1 cut(s) 622
PkrI GCNGC 6 cut(s) 253, 293, 322, 410, 630, 633
PleI GAGTC 1 cut(s) 406
PluTI GGCGCC 1 cut(s) 575
PpsI GAGTC 1 cut(s) 406
Ppu21I YACGTR 2 cut(s) 453, 544
Psp6I CCWGG 2 cut(s) 230, 402
PspFI CCCAGC 1 cut(s) 477
PspGI CCWGG 2 cut(s) 230, 402
PspN4I GGNNCC 3 cut(s) 184, 573, 787
PspOMI GGGCCC 1 cut(s) 785
PspPI GGNCC 3 cut(s) 276, 785, 786
PstNI CAGNNNCTG 1 cut(s) 634
PsuI RGATCY 2 cut(s) 111, 182
PsyI GACNNNGTC 1 cut(s) 622
PvuII CAGCTG 1 cut(s) 631
RsaI GTAC 8 cut(s) 53, 431, 501, 542, 551, 638, 677, 689
RsaNI GTAC 8 cut(s) 52, 430, 500, 541, 550, 637, 676, 688
RseI CAYNNNNRTG 1 cut(s) 425
Rsr2I CGGWCCG 1 cut(s) 276
RsrII CGGWCCG 1 cut(s) 276
SalI GTCGAC 1 cut(s) 4
SapI GCTCTTC 1 cut(s) 206
SatI GCNGC 6 cut(s) 252, 292, 321, 409, 629, 632
Sau3AI GATC 5 cut(s) 111, 139, 182, 465, 647
Sau96I GGNCC 3 cut(s) 276, 785, 786
ScaI AGTACT 1 cut(s) 53
SchI GAGTC 1 cut(s) 407
ScrFI CCNGG 3 cut(s) 232, 380, 404
SduI GDGCHC 2 cut(s) 591, 789
SfaNI GCATC 2 cut(s) 225, 294
SfoI GGCGCC 1 cut(s) 573
SinI GGWCC 1 cut(s) 276
SmiMI CAYNNNNRTG 1 cut(s) 425
Sse9I AATT 2 cut(s) 22, 553
SsiI CCGC 6 cut(s) 252, 289, 321, 352, 408, 474
SspDI GGCGCC 1 cut(s) 571
SspI AATATT 1 cut(s) 793
StyD4I CCNGG 3 cut(s) 230, 378, 402
StyI CCWWGG 2 cut(s) 64, 135
TaaI ACNGT 2 cut(s) 160, 586
TaiI ACGT 7 cut(s) 351, 372, 455, 546, 599, 614, 623
TaqI TCGA 2 cut(s) 5, 650
TaqII GACCGA 1 cut(s) 23
TasI AATT 2 cut(s) 22, 553
TatI WGTACW 3 cut(s) 51, 429, 549
TauI GCSGC 3 cut(s) 254, 323, 411
TfiI GAWTC 1 cut(s) 517
TscAI CASTG 1 cut(s) 591
TseFI GTSAC 2 cut(s) 565, 607
TseI GCWGC 3 cut(s) 291, 628, 631
Tsp45I GTSAC 2 cut(s) 565, 607
TspDTI ATGAA 2 cut(s) 154, 485
TspGWI ACGGA 1 cut(s) 195
TspRI CASTG 1 cut(s) 591
Tth111I GACNNNGTC 1 cut(s) 622
VneI GTGCAC 1 cut(s) 587
VpaK11BI GGWCC 1 cut(s) 276
XapI RAATTY 1 cut(s) 22
XceI RCATGY 1 cut(s) 40
XcmI CCANNNNNNNNNTGG 1 cut(s) 24
XmiI GTMKAC 1 cut(s) 5
ZraI GACGTC 1 cut(s) 621
ZrmI AGTACT 1 cut(s) 53
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.