RchiOBHm_Chr2g0137021

Serine aminopeptidase, S33

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0009854
Physical Location & Seq
Forward (+)
54481851 .. 54483574
1724 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ50780

Sequence Viewer

Length: 939 bp
ATGTCGACCGAAAAATCGGCACCGGCGCCGGAGACGCCTCCGAATTTCTGGGGTGACACGCCGGCGGAGGAGTACTACGCCTCCCAAGGGGTGCGCAACACTCAATCCTACTTCCCTACTCCCAACGGCCAGATCTTCACCCAGAGCTTCCTGCCGTTGGATCCCGACCTACCAGTCAAAGGCACCGTCTTCATGACCCACGGCTACGGCTCCGACACCGGTTGGCTCTTCCAGAAAATCTGCATCACCTTCTCCACCTGGGGCTACGCCGTCTTCGCCGCCGACCTCCTCGGCCACGGCCGCTCCGACGGCCTCCGCTGCTACCTCGGCGACATGGAGAAGGTCGCCGCCACCTCCCTCTCCTACTTCCTCCACGTCCGCCGCAGCGAGCCCTACCGCCGCCTCCCGGCCTTCCTCTTCGGCGAGTCGATGGGCGGCGCCGCTACCATGCTCATGTACTTCCAGTCCGACCCCGACACGTGGACGGGCCTGATCTTCTCGGCGCCGCTCTTCGTCATACCGGAGAACATGAAGCCCAGCAAGCTCCACCTCTTCGTCTACGGGCTCCTGTTCGGCCTCGCCGACACGTGGGCGGCGATGCCGGACAACAAGATGGTCGGAAAGGCCATCAAGGATCCCGAGAAGCTGAAAATCATAGCGTCCAATCCGAGGAGGGTGTGCCAGTACATACAGGATAACTTCTCCCGCGTAACGGCGCCGTTTCTGACCGTGCATGGGACTGCCGACGGGGTGACGTGTCCGACGTCGTCGAAGCTGTTGTATGAGAAGGCATCGAGTGTGGACAAGAGCTTGAAGATTTACGATGGGATGTACCATTCCTTGATACAAGGAGAGACTGATGAGAATGCCGACCTTGTGTTGAAGGATATGAGGGAGTGGATCGATGAGAGGGCGGAGAAGTATGGACCTAAACCCTAG

Protein Analysis

312

Amino Acids

34.87

Weight (kDa)

5.72

Isoelectric Point (pI)

30.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 59 - 285 2.4e-40 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 62 - 199 5.7e-07 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 64 - 279 2e-06 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009854)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G52760
fragaria_vesca FvH4_6g30610 FvH4_6g30610 FvH4_6g30610 FvH4_6g30610
malus_domestica MD17G1201900.v1.1
prunus_persica Prupe.5G109300_v2.0.a1
pyrus_communis pycom17g20580 pycom17g20590 pycom17g20600 pycom17g20610
rosa_chinensis RchiOBHm_Chr2g0137021
rosa_laevigata RLG00000019597
rosa_multiflora Rmu_sc0001374.1_g000047
rosa_roxburghii Rroxscaffold_2G00107770
rosa_rugosa Rorug02G0337200
rosa_samantha Rh2AG386300 Rh2BG393100 Rh2CG373600 Rh2DG409300
rosa_wichuraiana Rw2G031600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 173
AatII GACGTC 1 cut(s) 767
Acc16I TGCGCA 1 cut(s) 95
AccB1I GGYRCC 6 cut(s) 19, 25, 182, 437, 502, 715
AccBSI CCGCTC 2 cut(s) 303, 508
AccI GTMKAC 2 cut(s) 5, 558
AccII CGCG 1 cut(s) 708
AclWI GGATC 5 cut(s) 155, 168, 629, 642, 908
AcoI YGGCCR 3 cut(s) 127, 292, 298
AcsI RAATTY 1 cut(s) 43
AcvI CACGTG 2 cut(s) 480, 588
AcyI GRCGYC 6 cut(s) 26, 35, 438, 503, 716, 764
AfaI GTAC 4 cut(s) 74, 458, 686, 833
AfiI CCNNNNNNNGG 9 cut(s) 87, 157, 179, 406, 480, 588, 669, 712, 735
AflIII ACRYGT 3 cut(s) 477, 585, 755
AgeI ACCGGT 1 cut(s) 218
AgsI TTSAA 2 cut(s) 814, 883
AjiI CACGTC 2 cut(s) 376, 756
AjnI CCWGG 1 cut(s) 257
AluBI AGCT 5 cut(s) 147, 544, 646, 775, 810
AluI AGCT 5 cut(s) 147, 544, 646, 775, 810
Alw26I GTCTC 2 cut(s) 26, 848
AlwI GGATC 5 cut(s) 155, 168, 629, 642, 908
Ama87I CYCGRG 1 cut(s) 638
AoxI GGCC 8 cut(s) 127, 292, 298, 310, 408, 487, 574, 624
ApeKI GCWGC 2 cut(s) 318, 384
ApoI RAATTY 1 cut(s) 43
ArsI GACNNNNNNTTYG 2 cut(s) 171, 203
AsiGI ACCGGT 1 cut(s) 218
AspLEI GCGC 5 cut(s) 28, 96, 440, 505, 718
AspS9I GGNCC 2 cut(s) 487, 926
AsuC2I CCSGG 1 cut(s) 407
AsuHPI GGTGA 4 cut(s) 65, 130, 238, 763
AvaI CYCGRG 1 cut(s) 638
AvaII GGWCC 1 cut(s) 926
BamHI GGATCC 2 cut(s) 160, 634
BanI GGYRCC 6 cut(s) 19, 25, 182, 437, 502, 715
BanII GRGCYC 2 cut(s) 393, 567
BbrPI CACGTG 2 cut(s) 480, 588
BbsI GAAGAC 2 cut(s) 181, 265
BbvI GCAGC 2 cut(s) 305, 396
BccI CCATC 4 cut(s) 424, 607, 635, 818
BceAI ACGGC 9 cut(s) 139, 142, 217, 223, 254, 313, 325, 703, 729
BciT130I CCWGG 1 cut(s) 259
BcnI CCSGG 1 cut(s) 407
BcoDI GTCTC 2 cut(s) 26, 848
BfaI CTAG 1 cut(s) 937
BfoI RGCGCY 4 cut(s) 29, 441, 506, 719
BglII AGATCT 1 cut(s) 132
BmcAI AGTACT 1 cut(s) 74
Bme1390I CCNGG 2 cut(s) 259, 407
Bme18I GGWCC 1 cut(s) 926
BmeT110I CYCGRG 1 cut(s) 638
BmgBI CACGTC 2 cut(s) 376, 756
BmgT120I GGNCC 2 cut(s) 487, 926
BmrFI CCNGG 2 cut(s) 259, 407
BmsI GCATC 3 cut(s) 252, 588, 800
BpiI GAAGAC 2 cut(s) 181, 265
BpuMI CCSGG 1 cut(s) 407
Bsa29I ATCGAT 1 cut(s) 903
BsaAI YACGTR 2 cut(s) 480, 588
BsaHI GRCGYC 6 cut(s) 26, 35, 438, 503, 716, 764
BsaJI CCNNGG 7 cut(s) 85, 199, 258, 289, 295, 325, 668
BsaWI WCCGGW 2 cut(s) 218, 520
BsaXI ACNNNNNCTCC 4 cut(s) 65, 95, 287, 317
Bsc4I CCNNNNNNNGG 9 cut(s) 87, 157, 179, 406, 480, 588, 669, 712, 735
Bse118I RCCGGY 3 cut(s) 22, 61, 218
Bse1I ACTGG 3 cut(s) 173, 463, 682
BseBI CCWGG 1 cut(s) 259
BseCI ATCGAT 1 cut(s) 903
BseDI CCNNGG 7 cut(s) 85, 199, 258, 289, 295, 325, 668
BseGI GGATG 1 cut(s) 834
BseLI CCNNNNNNNGG 9 cut(s) 87, 157, 179, 406, 480, 588, 669, 712, 735
BseNI ACTGG 3 cut(s) 173, 463, 682
BseRI GAGGAG 3 cut(s) 83, 278, 685
BseX3I CGGCCG 1 cut(s) 298
BseXI GCAGC 2 cut(s) 305, 396
BseYI CCCAGC 1 cut(s) 536
Bsh1236I CGCG 1 cut(s) 708
Bsh1285I CGRYCG 2 cut(s) 9, 301
BshFI GGCC 8 cut(s) 129, 294, 300, 312, 410, 489, 576, 626
BshNI GGYRCC 6 cut(s) 19, 25, 182, 437, 502, 715
BshTI ACCGGT 1 cut(s) 218
BshVI ATCGAT 1 cut(s) 903
BsiEI CGRYCG 2 cut(s) 9, 301
BsiHKCI CYCGRG 1 cut(s) 638
BsiSI CCGG 7 cut(s) 23, 29, 62, 219, 407, 521, 602
BslFI GGGAC 1 cut(s) 751
BslI CCNNNNNNNGG 9 cut(s) 87, 157, 179, 406, 480, 588, 669, 712, 735
BsmAI GTCTC 2 cut(s) 26, 848
BsmBI CGTCTC 1 cut(s) 26
BsmFI GGGAC 1 cut(s) 751
BsmI GAATGC 1 cut(s) 871
BsnI GGCC 8 cut(s) 129, 294, 300, 312, 410, 489, 576, 626
BsoBI CYCGRG 1 cut(s) 638
Bsp1286I GDGCHC 2 cut(s) 393, 567
Bsp143I GATC 5 cut(s) 132, 160, 492, 634, 900
BspANI GGCC 8 cut(s) 129, 294, 300, 312, 410, 489, 576, 626
BspDI ATCGAT 1 cut(s) 903
BspFNI CGCG 1 cut(s) 708
BspHI TCATGA 1 cut(s) 192
BspPI GGATC 5 cut(s) 155, 168, 629, 642, 908
BspQI GCTCTTC 2 cut(s) 233, 515
BspT107I GGYRCC 6 cut(s) 19, 25, 182, 437, 502, 715
BsrBI CCGCTC 2 cut(s) 303, 508
BsrFI RCCGGY 3 cut(s) 22, 61, 218
BsrI ACTGG 3 cut(s) 173, 463, 682
BssAI RCCGGY 3 cut(s) 22, 61, 218
BssECI CCNNGG 7 cut(s) 85, 199, 258, 289, 295, 325, 668
BssMI GATC 5 cut(s) 132, 160, 492, 634, 900
BssNI GRCGYC 6 cut(s) 26, 35, 438, 503, 716, 764
BssT1I CCWWGG 1 cut(s) 85
Bst2UI CCWGG 1 cut(s) 259
Bst4CI ACNGT 2 cut(s) 187, 730
Bst6I CTCTTC 4 cut(s) 233, 422, 515, 557
BstACI GRCGYC 6 cut(s) 26, 35, 438, 503, 716, 764
BstBAI YACGTR 2 cut(s) 480, 588
BstC8I GCNNGC 3 cut(s) 63, 389, 542
BstDSI CCRYGG 2 cut(s) 199, 295
BstF5I GGATG 1 cut(s) 834
BstFNI CGCG 1 cut(s) 708
BstH2I RGCGCY 4 cut(s) 29, 441, 506, 719
BstHHI GCGC 5 cut(s) 28, 96, 440, 505, 718
BstKTI GATC 5 cut(s) 135, 163, 495, 637, 903
BstMAI GTCTC 2 cut(s) 26, 848
BstMBI GATC 5 cut(s) 132, 160, 492, 634, 900
BstMCI CGRYCG 2 cut(s) 9, 301
BstMWI GCNNNNNNNGC 7 cut(s) 34, 275, 300, 309, 318, 327, 541
BstNI CCWGG 1 cut(s) 259
BstSCI CCNGG 2 cut(s) 257, 405
BstUI CGCG 1 cut(s) 708
BstV1I GCAGC 2 cut(s) 305, 396
BstV2I GAAGAC 2 cut(s) 181, 265
BstX2I RGATCY 3 cut(s) 132, 160, 634
BstYI RGATCY 3 cut(s) 132, 160, 634
BstZI CGGCCG 1 cut(s) 298
Bsu15I ATCGAT 1 cut(s) 903
BsuRI GGCC 8 cut(s) 129, 294, 300, 312, 410, 489, 576, 626
BsuTUI ATCGAT 1 cut(s) 903
BtgI CCRYGG 2 cut(s) 199, 295
BtgZI GCGATG 1 cut(s) 611
BtrI CACGTC 2 cut(s) 376, 756
BtsCI GGATG 1 cut(s) 834
Cac8I GCNNGC 3 cut(s) 63, 389, 542
CciI TCATGA 1 cut(s) 192
CfoI GCGC 5 cut(s) 28, 96, 440, 505, 718
Cfr10I RCCGGY 3 cut(s) 22, 61, 218
Cfr13I GGNCC 2 cut(s) 487, 926
ClaI ATCGAT 1 cut(s) 903
CseI GACGC 2 cut(s) 43, 648
Csp6I GTAC 4 cut(s) 73, 457, 685, 832
CspAI ACCGGT 1 cut(s) 218
CviAII CATG 6 cut(s) 193, 334, 448, 454, 529, 734
CviQI GTAC 4 cut(s) 73, 457, 685, 832
DinI GGCGCC 4 cut(s) 27, 439, 504, 717
DpnI GATC 5 cut(s) 134, 162, 494, 636, 902
DpnII GATC 5 cut(s) 132, 160, 492, 634, 900
DrdI GACNNNNNNGTC 1 cut(s) 173
DseDI GACNNNNNNGTC 1 cut(s) 173
EaeI YGGCCR 3 cut(s) 127, 292, 298
EagI CGGCCG 1 cut(s) 298
Eam1104I CTCTTC 4 cut(s) 233, 422, 515, 557
EarI CTCTTC 4 cut(s) 233, 422, 515, 557
EciI GGCGGA 3 cut(s) 80, 368, 929
EclXI CGGCCG 1 cut(s) 298
Eco130I CCWWGG 1 cut(s) 85
Eco24I GRGCYC 2 cut(s) 393, 567
Eco47I GGWCC 1 cut(s) 926
Eco52I CGGCCG 1 cut(s) 298
Eco72I CACGTG 2 cut(s) 480, 588
Eco88I CYCGRG 1 cut(s) 638
EcoRII CCWGG 1 cut(s) 257
EcoT14I CCWWGG 1 cut(s) 85
EcoT38I GRGCYC 2 cut(s) 393, 567
EgeI GGCGCC 4 cut(s) 27, 439, 504, 717
EheI GGCGCC 4 cut(s) 27, 439, 504, 717
ErhI CCWWGG 1 cut(s) 85
Esp3I CGTCTC 1 cut(s) 26
FaeI CATG 6 cut(s) 196, 337, 451, 457, 532, 737
FaqI GGGAC 1 cut(s) 751
FatI CATG 6 cut(s) 192, 333, 447, 453, 528, 733
FauI CCCGC 1 cut(s) 713
FblI GTMKAC 2 cut(s) 5, 558
FokI GGATG 1 cut(s) 841
FriOI GRGCYC 2 cut(s) 393, 567
FspBI CTAG 1 cut(s) 937
FspI TGCGCA 1 cut(s) 95
GlaI GCGC 5 cut(s) 27, 95, 439, 504, 717
GsaI CCCAGC 1 cut(s) 540
HaeII RGCGCY 4 cut(s) 29, 441, 506, 719
HaeIII GGCC 8 cut(s) 129, 294, 300, 312, 410, 489, 576, 626
HapII CCGG 7 cut(s) 23, 29, 62, 219, 407, 521, 602
HgaI GACGC 2 cut(s) 43, 648
HhaI GCGC 5 cut(s) 28, 96, 440, 505, 718
Hin1I GRCGYC 6 cut(s) 26, 35, 438, 503, 716, 764
Hin1II CATG 6 cut(s) 196, 337, 451, 457, 532, 737
Hin6I GCGC 5 cut(s) 26, 94, 438, 503, 716
HinP1I GCGC 5 cut(s) 26, 94, 438, 503, 716
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HinfI GANTC 1 cut(s) 425
HpaII CCGG 7 cut(s) 23, 29, 62, 219, 407, 521, 602
HphI GGTGA 4 cut(s) 65, 130, 238, 763
Hpy166II GTNNAC 4 cut(s) 6, 483, 559, 802
Hpy188I TCNGA 8 cut(s) 42, 214, 307, 469, 620, 669, 726, 762
Hpy188III TCNNGA 4 cut(s) 164, 193, 232, 638
Hpy8I GTNNAC 4 cut(s) 6, 483, 559, 802
Hpy99I CGWCG 5 cut(s) 311, 749, 766, 769, 772
HpyAV CCTTC 5 cut(s) 259, 334, 421, 781, 877
HpyCH4III ACNGT 2 cut(s) 187, 730
HpyCH4IV ACGT 5 cut(s) 375, 479, 587, 755, 764
HpyCH4V TGCA 2 cut(s) 243, 733
HpyF10VI GCNNNNNNNGC 7 cut(s) 34, 275, 300, 309, 318, 327, 541
HpySE526I ACGT 5 cut(s) 375, 479, 587, 755, 764
Hsp92I GRCGYC 6 cut(s) 26, 35, 438, 503, 716, 764
Hsp92II CATG 6 cut(s) 196, 337, 451, 457, 532, 737
HspAI GCGC 5 cut(s) 26, 94, 438, 503, 716
KasI GGCGCC 4 cut(s) 25, 437, 502, 715
KroI GCCGGC 1 cut(s) 61
KroNI GCCGGC 1 cut(s) 63
Kzo9I GATC 5 cut(s) 132, 160, 492, 634, 900
LguI GCTCTTC 2 cut(s) 233, 515
LmnI GCTCC 4 cut(s) 215, 308, 549, 570
Lsp1109I GCAGC 2 cut(s) 305, 396
LweI GCATC 3 cut(s) 252, 588, 800
MaeI CTAG 1 cut(s) 937
MaeII ACGT 5 cut(s) 375, 479, 587, 755, 764
MaeIII GTNAC 3 cut(s) 53, 709, 751
MalI GATC 5 cut(s) 134, 162, 494, 636, 902
MbiI CCGCTC 2 cut(s) 303, 508
MboI GATC 5 cut(s) 132, 160, 492, 634, 900
MboII GAAGA 9 cut(s) 127, 181, 220, 265, 409, 487, 502, 544, 826
MflI RGATCY 3 cut(s) 132, 160, 634
MhlI GDGCHC 2 cut(s) 393, 567
MluCI AATT 1 cut(s) 43
Mly113I GGCGCC 4 cut(s) 26, 438, 503, 716
MlyI GAGTC 1 cut(s) 434
MmeI TCCRAC 6 cut(s) 138, 237, 330, 492, 598, 785
MreI CGCCGGCG 1 cut(s) 61
MroNI GCCGGC 1 cut(s) 61
MslI CAYNNNNRTG 1 cut(s) 452
MspA1I CMGCKG 1 cut(s) 318
MspI CCGG 7 cut(s) 23, 29, 62, 219, 407, 521, 602
MspR9I CCNGG 2 cut(s) 259, 407
Mva1269I GAATGC 1 cut(s) 871
MvaI CCWGG 1 cut(s) 259
MvnI CGCG 1 cut(s) 708
MwoI GCNNNNNNNGC 7 cut(s) 34, 275, 300, 309, 318, 327, 541
NaeI GCCGGC 1 cut(s) 63
NarI GGCGCC 4 cut(s) 26, 438, 503, 716
NciI CCSGG 1 cut(s) 407
NdeII GATC 5 cut(s) 132, 160, 492, 634, 900
NgoMIV GCCGGC 1 cut(s) 61
NlaIII CATG 6 cut(s) 196, 337, 451, 457, 532, 737
NmeAIII GCCGAG 3 cut(s) 270, 306, 479
NmuCI GTSAC 2 cut(s) 53, 751
NsbI TGCGCA 1 cut(s) 95
PagI TCATGA 1 cut(s) 192
PciSI GCTCTTC 2 cut(s) 233, 515
PcsI WCGNNNNNNNCGW 4 cut(s) 303, 579, 593, 761
PctI GAATGC 1 cut(s) 871
PdiI GCCGGC 1 cut(s) 63
PflFI GACNNNGTC 1 cut(s) 766
PinAI ACCGGT 1 cut(s) 218
PleI GAGTC 1 cut(s) 433
PluTI GGCGCC 4 cut(s) 29, 441, 506, 719
PmaCI CACGTG 2 cut(s) 480, 588
PmlI CACGTG 2 cut(s) 480, 588
PpsI GAGTC 1 cut(s) 433
Ppu21I YACGTR 2 cut(s) 480, 588
Psp6I CCWGG 1 cut(s) 257
PspCI CACGTG 2 cut(s) 480, 588
PspFI CCCAGC 1 cut(s) 536
PspGI CCWGG 1 cut(s) 257
PspPI GGNCC 2 cut(s) 487, 926
PsuI RGATCY 3 cut(s) 132, 160, 634
PsyI GACNNNGTC 1 cut(s) 766
RsaI GTAC 4 cut(s) 74, 458, 686, 833
RsaNI GTAC 4 cut(s) 73, 457, 685, 832
RseI CAYNNNNRTG 1 cut(s) 452
SalI GTCGAC 1 cut(s) 4
SapI GCTCTTC 2 cut(s) 233, 515
Sau3AI GATC 5 cut(s) 132, 160, 492, 634, 900
Sau96I GGNCC 2 cut(s) 487, 926
ScaI AGTACT 1 cut(s) 74
SchI GAGTC 1 cut(s) 434
ScrFI CCNGG 2 cut(s) 259, 407
SduI GDGCHC 2 cut(s) 393, 567
SfaNI GCATC 3 cut(s) 252, 588, 800
SfoI GGCGCC 4 cut(s) 27, 439, 504, 717
SgrAI CRCCGGYG 2 cut(s) 22, 61
SinI GGWCC 1 cut(s) 926
SmiMI CAYNNNNRTG 1 cut(s) 452
Sse9I AATT 1 cut(s) 43
SspDI GGCGCC 4 cut(s) 25, 437, 502, 715
SspMI CTAG 1 cut(s) 937
StyD4I CCNGG 2 cut(s) 257, 405
StyI CCWWGG 1 cut(s) 85
TaaI ACNGT 2 cut(s) 187, 730
TaiI ACGT 5 cut(s) 378, 482, 590, 758, 767
TaqI TCGA 5 cut(s) 5, 428, 770, 794, 903
TaqII GACCGA 1 cut(s) 23
TasI AATT 1 cut(s) 43
TatI WGTACW 3 cut(s) 72, 456, 684
TauI GCSGC 9 cut(s) 281, 303, 350, 384, 402, 438, 443, 508, 596
TseFI GTSAC 2 cut(s) 53, 751
TseI GCWGC 2 cut(s) 318, 384
Tsp45I GTSAC 2 cut(s) 53, 751
TspDTI ATGAA 2 cut(s) 181, 545
Tth111I GACNNNGTC 1 cut(s) 766
VpaK11BI GGWCC 1 cut(s) 926
XapI RAATTY 1 cut(s) 43
XmiI GTMKAC 2 cut(s) 5, 558
XspI CTAG 1 cut(s) 937
ZraI GACGTC 1 cut(s) 765
ZrmI AGTACT 1 cut(s) 74
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.