AT1G54140

Transcription initiation factor TFIID subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0012450
Physical Location & Seq
Reverse (-)
20213899 .. 20215265
1367 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G54140.1

Sequence Viewer

Length: 552 bp
ATGGCAGGAGAAGGTGAAGAAGATGTACCTAGAGATGCTAAGATTGTGAAATCTCTGCTTAAGTCGATGGGTGTAGAAGACTACGAGCCTCGTGTGATACATCAGTTTCTGGAGTTATGGTACCGGTATGTGGTCGAAGTGTTGACAGATGCTCAGGTTTACTCAGAGCATGCTAGCAAACCCAACATTGACTGTGATGATGTGAAGCTCGCTATTCAATCCAAAGTTAATTTCAGCTTCTCACAGCCTCCTCCAAGAGAGGTTCTGCTTGAGCTTGCTGCAAGTAGGAACAAGATACCTCTGCCAAAATCGATAGCAGGACCCGGTGTTCCACTCCCACCTGAACAGGACACATTGCTGAGCCCAAATTATCAGCTCGTGATACCGAAGAAGTCGGTTTCAACAGAACCTGAAGAAACCGAGGACGATGAAGAAATGACAGATCCTGGACAGTCCTCTCAAGAACAACAACAGCAGCAACAACAAACCTCAGACCTTCCAAGCCAAACTCCTCAAAGGGTCTCTTTCCCACTCTCTAGACGACCCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000123 GO:0000124 GO:0000428 GO:0001067 GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0005669 GO:0006139 GO:0006325 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006464 GO:0006473 GO:0006475 GO:0006725 GO:0006807 GO:0006996 GO:0007584 GO:0008134 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009605 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016043 GO:0016070 GO:0016569 GO:0016570 GO:0016573 GO:0016591 GO:0018130 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019219 GO:0019222 GO:0019438 GO:0019538 GO:0030880 GO:0031248 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031667 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0034708 GO:0035097 GO:0036211 GO:0042221 GO:0042493 GO:0043170 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043543 GO:0043966 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044665 GO:0044798 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051276 GO:0055029 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0070461 GO:0071339 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090575 GO:0097159 GO:0097659 GO:1901360 GO:1901362 GO:1901363 GO:1901564 GO:1901576 GO:1901698 GO:1901700 GO:1902065 GO:1902493 GO:1902494 GO:1902680 GO:1903506 GO:1903508 GO:1905368 GO:1990234 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03022 ko05168 map03022 map05168
Pfam Domains
Protein Families
TFIID-31kDa

Protein Analysis

183

Amino Acids

20.6

Weight (kDa)

4.67

Isoelectric Point (pI)

63.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TFIID-31kDa PF02291 7 - 126 2.2e-49 Transcription initiation factor IID, 31kD subunit
CENP-S PF15630 27 - 72 8.2e-06 CENP-S protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012450)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G54140
fragaria_vesca FvH4_6g34870 FvH4_6g34870
malus_domestica MD09G1181700.v1.1
prunus_persica Prupe.3G039800_v2.0.a1
pyrus_communis pycom09g09940
rosa_chinensis RchiOBHm_Chr2g0145741
rosa_laevigata RLG00000010130 RLG00000020165
rosa_multiflora Rmu_sc0000473.1_g000008
rosa_roxburghii Rroxscaffold_152G00434720 Rroxscaffold_2G00100480
rosa_rugosa Rorug02G0392900 Rorug02G0393000
rosa_samantha Rh2AG447300 Rh2BG459600 Rh2CG434300 Rh2DG469100
rosa_wichuraiana Rw2G036550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 120
AccB1I GGYRCC 1 cut(s) 120
AclWI GGATC 1 cut(s) 437
AcuI CTGAAG 1 cut(s) 432
AfaI GTAC 2 cut(s) 27, 122
AfiI CCNNNNNNNGG 1 cut(s) 130
AflII CTTAAG 1 cut(s) 59
AgeI ACCGGT 1 cut(s) 123
AgsI TTSAA 2 cut(s) 218, 402
AjnI CCWGG 1 cut(s) 445
AjuI GAANNNNNNNTTGG 2 cut(s) 215, 247
AloI GAACNNNNNNTCC 2 cut(s) 312, 344
AluBI AGCT 4 cut(s) 208, 237, 274, 376
AluI AGCT 4 cut(s) 208, 237, 274, 376
Alw26I GTCTC 1 cut(s) 526
AlwI GGATC 1 cut(s) 437
AlwNI CAGNNNCTG 3 cut(s) 109, 410, 446
ApeKI GCWGC 2 cut(s) 278, 475
AsiGI ACCGGT 1 cut(s) 123
Asp718I GGTACC 1 cut(s) 120
AspS9I GGNCC 1 cut(s) 320
AsuC2I CCSGG 1 cut(s) 324
AsuHPI GGTGA 1 cut(s) 26
AsuNHI GCTAGC 1 cut(s) 173
AvaII GGWCC 1 cut(s) 320
BaeI ACNNNNGTAYC 2 cut(s) 89, 122
BanI GGYRCC 1 cut(s) 120
BanII GRGCYC 1 cut(s) 365
BarI GAAGNNNNNNTAC 2 cut(s) 9, 41
BauI CACGAG 2 cut(s) 90, 377
BbsI GAAGAC 1 cut(s) 84
BbvI GCAGC 2 cut(s) 265, 487
BccI CCATC 1 cut(s) 61
BciT130I CCWGG 1 cut(s) 447
BcnI CCSGG 1 cut(s) 324
BcoDI GTCTC 1 cut(s) 526
BfaI CTAG 3 cut(s) 30, 174, 537
BfrI CTTAAG 1 cut(s) 59
BisI GCNGC 2 cut(s) 279, 476
BlpI GCTNAGC 1 cut(s) 359
BlsI GCNGC 2 cut(s) 280, 477
Bme1390I CCNGG 2 cut(s) 324, 447
Bme18I GGWCC 1 cut(s) 320
BmgT120I GGNCC 1 cut(s) 320
BmiI GGNNCC 2 cut(s) 122, 322
BmrFI CCNGG 2 cut(s) 324, 447
BmsI GCATC 2 cut(s) 25, 139
BmtI GCTAGC 1 cut(s) 177
BpiI GAAGAC 1 cut(s) 84
BpmI CTGGAG 1 cut(s) 131
Bpu10I CCTNAGC 1 cut(s) 153
Bpu1102I GCTNAGC 1 cut(s) 359
BpuEI CTTGAG 2 cut(s) 290, 444
BpuMI CCSGG 1 cut(s) 324
Bsa29I ATCGAT 1 cut(s) 311
BsaI GGTCTC 1 cut(s) 526
BsaJI CCNNGG 1 cut(s) 420
BsaWI WCCGGW 1 cut(s) 123
BsaXI ACNNNNNCTCC 2 cut(s) 104, 134
Bsc4I CCNNNNNNNGG 1 cut(s) 130
Bse118I RCCGGY 1 cut(s) 123
Bse3DI GCAATG 1 cut(s) 353
BseBI CCWGG 1 cut(s) 447
BseCI ATCGAT 1 cut(s) 311
BseDI CCNNGG 1 cut(s) 420
BseLI CCNNNNNNNGG 1 cut(s) 130
BseMI GCAATG 1 cut(s) 353
BseMII CTCAG 4 cut(s) 167, 177, 350, 504
BseRI GAGGAG 2 cut(s) 240, 501
BseXI GCAGC 2 cut(s) 265, 487
BshNI GGYRCC 1 cut(s) 120
BshTI ACCGGT 1 cut(s) 123
BshVI ATCGAT 1 cut(s) 311
BsiSI CCGG 2 cut(s) 124, 324
BslI CCNNNNNNNGG 1 cut(s) 130
BsmAI GTCTC 1 cut(s) 526
Bso31I GGTCTC 1 cut(s) 526
Bsp1286I GDGCHC 1 cut(s) 365
Bsp143I GATC 1 cut(s) 442
Bsp1720I GCTNAGC 1 cut(s) 359
BspCNI CTCAG 4 cut(s) 166, 176, 351, 503
BspDI ATCGAT 1 cut(s) 311
BspLI GGNNCC 2 cut(s) 122, 322
BspOI GCTAGC 1 cut(s) 177
BspPI GGATC 1 cut(s) 437
BspT107I GGYRCC 1 cut(s) 120
BspTI CTTAAG 1 cut(s) 59
BspTNI GGTCTC 1 cut(s) 526
BsrDI GCAATG 1 cut(s) 353
BsrFI RCCGGY 1 cut(s) 123
BssAI RCCGGY 1 cut(s) 123
BssECI CCNNGG 1 cut(s) 420
BssMI GATC 1 cut(s) 442
BssSI CACGAG 2 cut(s) 90, 377
Bst2BI CACGAG 2 cut(s) 90, 377
Bst2UI CCWGG 1 cut(s) 447
Bst4CI ACNGT 2 cut(s) 194, 453
BstAFI CTTAAG 1 cut(s) 59
BstC8I GCNNGC 4 cut(s) 171, 175, 210, 276
BstDEI CTNAG 5 cut(s) 39, 153, 163, 359, 490
BstKTI GATC 1 cut(s) 445
BstMAI GTCTC 1 cut(s) 526
BstMBI GATC 1 cut(s) 442
BstNI CCWGG 1 cut(s) 447
BstNSI RCATGY 1 cut(s) 173
BstSCI CCNGG 2 cut(s) 322, 445
BstV1I GCAGC 2 cut(s) 265, 487
BstV2I GAAGAC 1 cut(s) 84
BstX2I RGATCY 1 cut(s) 442
BstYI RGATCY 1 cut(s) 442
Bsu15I ATCGAT 1 cut(s) 311
BsuTUI ATCGAT 1 cut(s) 311
Cac8I GCNNGC 4 cut(s) 171, 175, 210, 276
CaiI CAGNNNCTG 3 cut(s) 109, 410, 446
Cfr10I RCCGGY 1 cut(s) 123
Cfr13I GGNCC 1 cut(s) 320
ClaI ATCGAT 1 cut(s) 311
Csp6I GTAC 2 cut(s) 26, 121
CspAI ACCGGT 1 cut(s) 123
CviAII CATG 1 cut(s) 170
CviJI RGCY 8 cut(s) 88, 208, 237, 247, 274, 363, 376, 504
CviKI_1 RGCY 8 cut(s) 88, 208, 237, 247, 274, 363, 376, 504
CviQI GTAC 2 cut(s) 26, 121
DdeI CTNAG 5 cut(s) 39, 153, 163, 359, 490
DpnI GATC 1 cut(s) 444
DpnII GATC 1 cut(s) 442
Eco24I GRGCYC 1 cut(s) 365
Eco31I GGTCTC 1 cut(s) 526
Eco47I GGWCC 1 cut(s) 320
Eco57I CTGAAG 1 cut(s) 432
EcoO109I RGGNCCY 1 cut(s) 320
EcoRII CCWGG 1 cut(s) 445
EcoT38I GRGCYC 1 cut(s) 365
FaeI CATG 1 cut(s) 173
FaiI YATR 3 cut(s) 118, 129, 171
FalI AAGNNNNNCTT 2 cut(s) 508, 540
FatI CATG 1 cut(s) 169
Fnu4HI GCNGC 2 cut(s) 279, 476
FriOI GRGCYC 1 cut(s) 365
Fsp4HI GCNGC 2 cut(s) 279, 476
FspBI CTAG 3 cut(s) 30, 174, 537
GluI GCNGC 2 cut(s) 279, 476
GsuI CTGGAG 1 cut(s) 131
HapII CCGG 2 cut(s) 124, 324
Hin1II CATG 1 cut(s) 173
HincII GTYRAC 1 cut(s) 144
HindII GTYRAC 1 cut(s) 144
HpaII CCGG 2 cut(s) 124, 324
HphI GGTGA 1 cut(s) 26
Hpy166II GTNNAC 2 cut(s) 144, 160
Hpy188I TCNGA 2 cut(s) 166, 493
Hpy188III TCNNGA 4 cut(s) 110, 379, 461, 537
Hpy8I GTNNAC 2 cut(s) 144, 160
HpyAV CCTTC 2 cut(s) 5, 506
HpyCH4III ACNGT 2 cut(s) 194, 453
HpyCH4V TGCA 1 cut(s) 281
HpyF3I CTNAG 5 cut(s) 39, 153, 163, 359, 490
Hsp92II CATG 1 cut(s) 173
KpnI GGTACC 1 cut(s) 124
Kzo9I GATC 1 cut(s) 442
Lsp1109I GCAGC 2 cut(s) 265, 487
LweI GCATC 2 cut(s) 25, 139
MaeI CTAG 3 cut(s) 30, 174, 537
MalI GATC 1 cut(s) 444
MboI GATC 1 cut(s) 442
MboII GAAGA 6 cut(s) 29, 32, 89, 400, 425, 443
MflI RGATCY 1 cut(s) 442
MhlI GDGCHC 1 cut(s) 365
MluCI AATT 2 cut(s) 229, 367
MnlI CCTC 9 cut(s) 99, 253, 258, 261, 309, 415, 466, 499, 522
MseI TTAA 2 cut(s) 60, 228
MspCI CTTAAG 1 cut(s) 59
MspI CCGG 2 cut(s) 124, 324
MspR9I CCNGG 2 cut(s) 324, 447
MvaI CCWGG 1 cut(s) 447
NciI CCSGG 1 cut(s) 324
NdeII GATC 1 cut(s) 442
NheI GCTAGC 1 cut(s) 173
NlaIII CATG 1 cut(s) 173
NlaIV GGNNCC 2 cut(s) 122, 322
NspI RCATGY 1 cut(s) 173
PaeI GCATGC 1 cut(s) 173
PfoI TCCNGGA 1 cut(s) 445
PinAI ACCGGT 1 cut(s) 123
PkrI GCNGC 2 cut(s) 280, 477
PpuMI RGGWCCY 1 cut(s) 320
Psp5II RGGWCCY 1 cut(s) 320
Psp6I CCWGG 1 cut(s) 445
PspGI CCWGG 1 cut(s) 445
PspN4I GGNNCC 2 cut(s) 122, 322
PspPI GGNCC 1 cut(s) 320
PspPPI RGGWCCY 1 cut(s) 320
PstNI CAGNNNCTG 3 cut(s) 109, 410, 446
PsuI RGATCY 1 cut(s) 442
RsaI GTAC 2 cut(s) 27, 122
RsaNI GTAC 2 cut(s) 26, 121
SaqAI TTAA 2 cut(s) 60, 228
SatI GCNGC 2 cut(s) 279, 476
Sau3AI GATC 1 cut(s) 442
Sau96I GGNCC 1 cut(s) 320
ScrFI CCNGG 2 cut(s) 324, 447
SduI GDGCHC 1 cut(s) 365
SfaNI GCATC 2 cut(s) 25, 139
SinI GGWCC 1 cut(s) 320
SmlI CTYRAG 3 cut(s) 59, 269, 459
SmoI CTYRAG 3 cut(s) 59, 269, 459
SphI GCATGC 1 cut(s) 173
Sse9I AATT 2 cut(s) 229, 367
SspMI CTAG 3 cut(s) 30, 174, 537
StyD4I CCNGG 2 cut(s) 322, 445
TaaI ACNGT 2 cut(s) 194, 453
TaqI TCGA 3 cut(s) 65, 135, 311
TasI AATT 2 cut(s) 229, 367
Tru1I TTAA 2 cut(s) 60, 228
Tru9I TTAA 2 cut(s) 60, 228
TseI GCWGC 2 cut(s) 278, 475
TspDTI ATGAA 1 cut(s) 444
Vha464I CTTAAG 1 cut(s) 59
VpaK11BI GGWCC 1 cut(s) 320
XbaI TCTAGA 1 cut(s) 536
XceI RCATGY 1 cut(s) 173
XspI CTAG 3 cut(s) 30, 174, 537
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.