AT1G56350

peptide chain release factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0014362
Physical Location & Seq
Forward (+)
21094743 .. 21098047
3305 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G56350.1

Sequence Viewer

Length: 1449 bp
ATGTTATCGTTAATCATCAGAAGGTCTCGAAGCAGATTCATCATCCATGGAATCAAAATTTCTTGTAACTCTCTGTCGGCTGTAGATTCAAGTTCTTCAGTTTTGGTTTCTAGAAGAACCTTCAGTTCAACACCTGCGTTAAGCTATTTTGGATTCAATTATGGAAAAATCTCTAATTTGAATCAGAGATTTGGTAATTACGCTTATTCTGGGAGTAGTTTCGCTTCCAGTACTCGATCATTGAGCTCAGAAGCTGTAGCTGTGGCAGCGACTTGTGATGGACTTACTGTGGAACGAATTATAGCTAATCAGTGGCCGATTTTAGATGAGAATGAAGGTGATTGGAAGAGTCACGCTGCAGCAATTGCACAGTCTATTCAAGTCATCAAGAGACGATTACAGTGGAAGAAACTCTTGGTGAGGCTGAAAGTTTTATCTGCAGAGTTGAACAAGTCTGATCTTTGGGATGATCCAACTCATGCTGGTAAGATAAGCCGGGAGCACGGCTCGCTTACGGGAAAAATGAAAGGAGTTATGACATTTGAGAGAGAGCTTCTTGAACACATTGACATGTTGAAACTCGCTAAAGAGGAGAACGATTCAGAGTTAGAATCGGAAACTTTAAAGGCATTGATAGATATGAGAAGGGTGTCAAAAGAGAAAGAACTCGAGGCTTTATTATCTGCGGATAATGATCCTTGTTCTTGTTATATCGAGGTTCAAGCTGGAGCTGGAGGAACAGAGAGCAATGATTGGGCAGCTATGGTAATGGAAATGTACAAAACATGGGCTCAACGACGCAAATTTTCAGTCACTGTGGTTGATGAAGCTCCTGGGGAGATAGCAGGAATCAAGCGTGCTACAATCAAAGTGAACGGTGAATACGCATACGGATACGCCAAAGCAGAAGTTGGAGTACATAGGCTGGTGCGTATATCCCCGTTTGACAGTGGAAAACGGAGACACACTTCGTTTGCGGCAGTTGCGGTGATTCCAATTCTAGGTGATGGGTCCACCCGTGTGGAGATCAATGACTCCGATCTCCGGATTGAGCGGTTCCGTTCTGGAGGAGCCGGTGGGCAGCACGCTAACACGACAGACAGTGCTGTTAGAATCGTTCACATACCAACTGGCATAACAGCAACTTGTCAGAATGAAAGGTCACAACATAGTAACAAGGCTTCAGCAATGGCAGTACTACAATCACGGTTAGACCAACTGGAAATGGCTCGTCAGACAGCAATGAACGCTCAACATACACAATCACTAACAGAGATCAGCTGGGGAAACCAGATACGAACTTATGTTCTCCATCCATACAGAATGGTGAAGGATCTGCGAACCAACTACGAGGTCTCTGATCCTGACTCGGTTCTTGAAGGAGACTTAGATGGTTTCATCTTGAGCTTCTTATCTTCTTCTTTAGACAAAGATGATCCTGAACATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PCRF RF-1

Protein Analysis

482

Amino Acids

53.43

Weight (kDa)

6.91

Isoelectric Point (pI)

45.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCRF PF03462 141 - 328 2.2e-50 PCRF domain
RF-1 PF00472 340 - 447 1.1e-37 RF-1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014362)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G56350
fragaria_vesca FvH4_6g07390
malus_domestica MD04G1193600.v1.1 MD12G1206300.v1.1
prunus_persica Prupe.6G313600_v2.0.a1 Prupe.6G313600_v2.0.a1
pyrus_communis pycom12g19420
rosa_chinensis RchiOBHm_Chr3g0456991
rosa_laevigata RLG00000025255
rosa_multiflora Rmu_co8479901.1_g000001
rosa_roxburghii Rroxscaffold_6G00422290
rosa_rugosa Rorug03G0018100
rosa_samantha Rh3AG077900 Rh3BG079900 Rh3CG079800 Rh3DG081600
rosa_wichuraiana Rw3G006690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 142
Acc36I ACCTGC 1 cut(s) 142
AccBSI CCGCTC 1 cut(s) 1054
AccIII TCCGGA 1 cut(s) 1044
AciI CCGC 4 cut(s) 686, 977, 986, 1054
AclWI GGATC 5 cut(s) 464, 689, 1341, 1355, 1430
AcoI YGGCCR 1 cut(s) 314
AcsI RAATTY 2 cut(s) 57, 803
AcuI CTGAAG 3 cut(s) 81, 106, 1167
AfaI GTAC 4 cut(s) 232, 779, 918, 1197
AfiI CCNNNNNNNGG 2 cut(s) 1001, 1044
AflIII ACRYGT 1 cut(s) 570
AjnI CCWGG 1 cut(s) 832
AjuI GAANNNNNNNTTGG 2 cut(s) 398, 430
AleI CACNNNNGTG 1 cut(s) 1019
Alw21I GWGCWC 2 cut(s) 248, 504
Alw26I GTCTC 5 cut(s) 30, 385, 955, 1360, 1377
AlwI GGATC 5 cut(s) 464, 689, 1341, 1355, 1430
AlwNI CAGNNNCTG 2 cut(s) 254, 815
Ama87I CYCGRG 1 cut(s) 668
Aor13HI TCCGGA 1 cut(s) 1044
AoxI GGCC 1 cut(s) 314
ApeKI GCWGC 5 cut(s) 266, 356, 359, 758, 1081
ApoI RAATTY 2 cut(s) 57, 803
ArsI GACNNNNNNTTYG 2 cut(s) 795, 827
AspS9I GGNCC 1 cut(s) 1011
AsuC2I CCSGG 1 cut(s) 497
AsuHPI GGTGA 6 cut(s) 350, 430, 890, 1000, 1016, 1339
AvaI CYCGRG 1 cut(s) 668
AvaII GGWCC 1 cut(s) 1011
BanII GRGCYC 2 cut(s) 248, 793
BarI GAAGNNNNNNTAC 6 cut(s) 208, 240, 900, 932, 1165, 1197
Bbv12I GWGCWC 2 cut(s) 248, 504
BbvI GCAGC 5 cut(s) 278, 343, 371, 770, 1093
BccI CCATC 4 cut(s) 272, 1001, 1320, 1385
BceAI ACGGC 1 cut(s) 520
BciT130I CCWGG 1 cut(s) 834
BciVI GTATCC 1 cut(s) 887
BcnI CCSGG 1 cut(s) 497
BcoDI GTCTC 5 cut(s) 30, 385, 955, 1360, 1377
BfaI CTAG 2 cut(s) 111, 1001
BfmI CTRYAG 4 cut(s) 81, 255, 357, 438
BfuAI ACCTGC 1 cut(s) 142
BfuI GTATCC 1 cut(s) 887
BisI GCNGC 6 cut(s) 267, 357, 360, 759, 978, 1082
BlsI GCNGC 6 cut(s) 268, 358, 361, 760, 979, 1083
BmcAI AGTACT 2 cut(s) 232, 1197
Bme1390I CCNGG 2 cut(s) 497, 834
Bme18I GGWCC 1 cut(s) 1011
BmeT110I CYCGRG 1 cut(s) 668
BmgT120I GGNCC 1 cut(s) 1011
BmiI GGNNCC 3 cut(s) 1012, 1058, 1072
BmrFI CCNGG 2 cut(s) 497, 834
BplI GAGNNNNNCTC 2 cut(s) 491, 523
BpmI CTGGAG 3 cut(s) 747, 753, 1086
BpuEI CTTGAG 1 cut(s) 1423
BpuMI CCSGG 1 cut(s) 497
BsaBI GATNNNNATC 1 cut(s) 693
BsaI GGTCTC 2 cut(s) 30, 1360
BsaJI CCNNGG 2 cut(s) 46, 833
BsaWI WCCGGW 1 cut(s) 1044
Bsc4I CCNNNNNNNGG 2 cut(s) 1001, 1044
Bse118I RCCGGY 1 cut(s) 1073
Bse1I ACTGG 3 cut(s) 228, 1135, 1224
Bse3DI GCAATG 3 cut(s) 754, 1194, 1248
Bse8I GATNNNNATC 1 cut(s) 693
BseAI TCCGGA 1 cut(s) 1044
BseBI CCWGG 1 cut(s) 834
BseDI CCNNGG 2 cut(s) 46, 833
BseGI GGATG 3 cut(s) 42, 472, 1312
BseJI GATNNNNATC 1 cut(s) 693
BseLI CCNNNNNNNGG 2 cut(s) 1001, 1044
BseMI GCAATG 3 cut(s) 754, 1194, 1248
BseMII CTCAG 1 cut(s) 261
BseNI ACTGG 3 cut(s) 228, 1135, 1224
BseRI GAGGAG 2 cut(s) 605, 1083
BseXI GCAGC 5 cut(s) 278, 343, 371, 770, 1093
BseYI CCCAGC 1 cut(s) 1281
BshFI GGCC 1 cut(s) 316
BsiHKAI GWGCWC 2 cut(s) 248, 504
BsiHKCI CYCGRG 1 cut(s) 668
BsiSI CCGG 3 cut(s) 496, 1045, 1074
BslI CCNNNNNNNGG 2 cut(s) 1001, 1044
BsmAI GTCTC 5 cut(s) 30, 385, 955, 1360, 1377
BsmBI CGTCTC 1 cut(s) 385
BsnI GGCC 1 cut(s) 316
Bso31I GGTCTC 2 cut(s) 30, 1360
BsoBI CYCGRG 1 cut(s) 668
Bsp1286I GDGCHC 3 cut(s) 248, 504, 793
Bsp13I TCCGGA 1 cut(s) 1044
Bsp1407I TGTACA 1 cut(s) 777
Bsp19I CCATGG 1 cut(s) 46
BspACI CCGC 4 cut(s) 686, 977, 986, 1054
BspANI GGCC 1 cut(s) 316
BspCNI CTCAG 1 cut(s) 260
BspEI TCCGGA 1 cut(s) 1044
BspLI GGNNCC 3 cut(s) 1012, 1058, 1072
BspMAI CTGCAG 2 cut(s) 361, 442
BspMI ACCTGC 1 cut(s) 142
BspPI GGATC 5 cut(s) 464, 689, 1341, 1355, 1430
BspTNI GGTCTC 2 cut(s) 30, 1360
BsrBI CCGCTC 1 cut(s) 1054
BsrDI GCAATG 3 cut(s) 754, 1194, 1248
BsrFI RCCGGY 1 cut(s) 1073
BsrGI TGTACA 1 cut(s) 777
BsrI ACTGG 3 cut(s) 228, 1135, 1224
BssAI RCCGGY 1 cut(s) 1073
BssECI CCNNGG 2 cut(s) 46, 833
BssT1I CCWWGG 1 cut(s) 46
Bst2UI CCWGG 1 cut(s) 834
Bst4CI ACNGT 8 cut(s) 289, 372, 402, 817, 878, 950, 1103, 1209
Bst6I CTCTTC 1 cut(s) 341
BstAPI GCANNNNNTGC 1 cut(s) 365
BstAUI TGTACA 1 cut(s) 777
BstC8I GCNNGC 3 cut(s) 509, 858, 1086
BstDEI CTNAG 2 cut(s) 247, 1387
BstDSI CCRYGG 1 cut(s) 46
BstF5I GGATG 3 cut(s) 42, 472, 1312
BstMAI GTCTC 5 cut(s) 30, 385, 955, 1360, 1377
BstMWI GCNNNNNNNGC 5 cut(s) 266, 365, 508, 983, 1247
BstNI CCWGG 1 cut(s) 834
BstNSI RCATGY 1 cut(s) 574
BstSCI CCNGG 2 cut(s) 495, 832
BstSFI CTRYAG 4 cut(s) 81, 255, 357, 438
BstV1I GCAGC 5 cut(s) 278, 343, 371, 770, 1093
BstX2I RGATCY 1 cut(s) 1333
BstXI CCANNNNNNTGG 1 cut(s) 1021
BstYI RGATCY 1 cut(s) 1333
BsuI GTATCC 1 cut(s) 887
BsuRI GGCC 1 cut(s) 316
BtgI CCRYGG 1 cut(s) 46
BtsCI GGATG 3 cut(s) 42, 472, 1312
BtsIMutI CAGTG 5 cut(s) 317, 407, 813, 955, 1108
BveI ACCTGC 1 cut(s) 142
Cac8I GCNNGC 3 cut(s) 509, 858, 1086
CaiI CAGNNNCTG 2 cut(s) 254, 815
Cfr10I RCCGGY 1 cut(s) 1073
Cfr13I GGNCC 1 cut(s) 1011
CseI GACGC 1 cut(s) 807
Csp6I GTAC 4 cut(s) 231, 778, 917, 1196
CviAII CATG 4 cut(s) 47, 479, 571, 786
CviQI GTAC 4 cut(s) 231, 778, 917, 1196
DdeI CTNAG 2 cut(s) 247, 1387
DraI TTTAAA 1 cut(s) 624
EaeI YGGCCR 1 cut(s) 314
Eam1104I CTCTTC 1 cut(s) 341
EarI CTCTTC 1 cut(s) 341
Ecl136II GAGCTC 1 cut(s) 246
Eco130I CCWWGG 1 cut(s) 46
Eco24I GRGCYC 2 cut(s) 248, 793
Eco31I GGTCTC 2 cut(s) 30, 1360
Eco47I GGWCC 1 cut(s) 1011
Eco53kI GAGCTC 1 cut(s) 246
Eco57I CTGAAG 3 cut(s) 81, 106, 1167
Eco88I CYCGRG 1 cut(s) 668
EcoICRI GAGCTC 1 cut(s) 246
EcoRII CCWGG 1 cut(s) 832
EcoT14I CCWWGG 1 cut(s) 46
EcoT38I GRGCYC 2 cut(s) 248, 793
ErhI CCWWGG 1 cut(s) 46
Esp3I CGTCTC 1 cut(s) 385
FaeI CATG 4 cut(s) 50, 482, 574, 789
FalI AAGNNNNNCTT 2 cut(s) 398, 430
FatI CATG 4 cut(s) 46, 478, 570, 785
Fnu4HI GCNGC 6 cut(s) 267, 357, 360, 759, 978, 1082
FokI GGATG 3 cut(s) 29, 479, 1299
FriOI GRGCYC 2 cut(s) 248, 793
Fsp4HI GCNGC 6 cut(s) 267, 357, 360, 759, 978, 1082
FspBI CTAG 2 cut(s) 111, 1001
GluI GCNGC 6 cut(s) 267, 357, 360, 759, 978, 1082
GsaI CCCAGC 1 cut(s) 1285
GsuI CTGGAG 3 cut(s) 747, 753, 1086
HaeIII GGCC 1 cut(s) 316
HapII CCGG 3 cut(s) 496, 1045, 1074
HgaI GACGC 1 cut(s) 807
Hin1II CATG 4 cut(s) 50, 482, 574, 789
HpaII CCGG 3 cut(s) 496, 1045, 1074
HphI GGTGA 6 cut(s) 350, 430, 890, 1000, 1016, 1339
Hpy166II GTNNAC 3 cut(s) 874, 1014, 1120
Hpy8I GTNNAC 3 cut(s) 874, 1014, 1120
Hpy99I CGWCG 1 cut(s) 801
HpyAV CCTTC 6 cut(s) 15, 130, 329, 639, 1324, 1373
HpyCH4III ACNGT 8 cut(s) 289, 372, 402, 817, 878, 950, 1103, 1209
HpyCH4V TGCA 3 cut(s) 359, 368, 440
HpyF10VI GCNNNNNNNGC 5 cut(s) 266, 365, 508, 983, 1247
HpyF3I CTNAG 2 cut(s) 247, 1387
Hsp92II CATG 4 cut(s) 50, 482, 574, 789
Kpn2I TCCGGA 1 cut(s) 1044
LmnI GCTCC 4 cut(s) 499, 728, 835, 1070
Lsp1109I GCAGC 5 cut(s) 278, 343, 371, 770, 1093
MaeI CTAG 2 cut(s) 111, 1001
MaeIII GTNAC 5 cut(s) 65, 350, 811, 1161, 1172
MbiI CCGCTC 1 cut(s) 1054
MboII GAAGA 6 cut(s) 87, 126, 358, 418, 1407, 1410
MfeI CAATTG 1 cut(s) 363
MflI RGATCY 1 cut(s) 1333
MhlI GDGCHC 3 cut(s) 248, 504, 793
MluCI AATT 8 cut(s) 57, 157, 175, 196, 297, 363, 803, 996
MlyI GAGTC 3 cut(s) 358, 1028, 1361
MmeI TCCRAC 2 cut(s) 497, 892
MnlI CCTC 7 cut(s) 414, 583, 664, 709, 728, 1061, 1345
MroI TCCGGA 1 cut(s) 1044
MseI TTAA 3 cut(s) 11, 140, 623
MslI CAYNNNNRTG 2 cut(s) 569, 1019
MspA1I CMGCKG 1 cut(s) 1281
MspI CCGG 3 cut(s) 496, 1045, 1074
MspR9I CCNGG 2 cut(s) 497, 834
MunI CAATTG 1 cut(s) 363
MvaI CCWGG 1 cut(s) 834
MwoI GCNNNNNNNGC 5 cut(s) 266, 365, 508, 983, 1247
NciI CCSGG 1 cut(s) 497
NcoI CCATGG 1 cut(s) 46
NlaIII CATG 4 cut(s) 50, 482, 574, 789
NlaIV GGNNCC 3 cut(s) 1012, 1058, 1072
NmuCI GTSAC 3 cut(s) 350, 811, 1161
NspI RCATGY 1 cut(s) 574
OliI CACNNNNGTG 1 cut(s) 1019
PaeR7I CTCGAG 1 cut(s) 668
PaqCI CACCTGC 1 cut(s) 142
PciI ACATGT 1 cut(s) 570
PkrI GCNGC 6 cut(s) 268, 358, 361, 760, 979, 1083
PleI GAGTC 3 cut(s) 357, 1028, 1361
PpsI GAGTC 3 cut(s) 357, 1028, 1361
PscI ACATGT 1 cut(s) 570
Psp124BI GAGCTC 1 cut(s) 248
Psp6I CCWGG 1 cut(s) 832
PspFI CCCAGC 1 cut(s) 1281
PspGI CCWGG 1 cut(s) 832
PspN4I GGNNCC 3 cut(s) 1012, 1058, 1072
PspPI GGNCC 1 cut(s) 1011
PspXI VCTCGAGB 1 cut(s) 668
PsrI GAACNNNNNNTAC 2 cut(s) 866, 898
PstI CTGCAG 2 cut(s) 361, 442
PstNI CAGNNNCTG 2 cut(s) 254, 815
PsuI RGATCY 1 cut(s) 1333
PvuII CAGCTG 1 cut(s) 1281
RsaI GTAC 4 cut(s) 232, 779, 918, 1197
RsaNI GTAC 4 cut(s) 231, 778, 917, 1196
RseI CAYNNNNRTG 2 cut(s) 569, 1019
SacI GAGCTC 1 cut(s) 248
SaqAI TTAA 3 cut(s) 11, 140, 623
SatI GCNGC 6 cut(s) 267, 357, 360, 759, 978, 1082
Sau96I GGNCC 1 cut(s) 1011
ScaI AGTACT 2 cut(s) 232, 1197
SchI GAGTC 3 cut(s) 358, 1028, 1361
ScrFI CCNGG 2 cut(s) 497, 834
SduI GDGCHC 3 cut(s) 248, 504, 793
SfcI CTRYAG 4 cut(s) 81, 255, 357, 438
Sfr274I CTCGAG 1 cut(s) 668
SinI GGWCC 1 cut(s) 1011
SlaI CTCGAG 1 cut(s) 668
SmiMI CAYNNNNRTG 2 cut(s) 569, 1019
SmlI CTYRAG 2 cut(s) 668, 1402
SmoI CTYRAG 2 cut(s) 668, 1402
Sse9I AATT 8 cut(s) 57, 157, 175, 196, 297, 363, 803, 996
SsiI CCGC 4 cut(s) 686, 977, 986, 1054
SspMI CTAG 2 cut(s) 111, 1001
SstI GAGCTC 1 cut(s) 248
StyD4I CCNGG 2 cut(s) 495, 832
StyI CCWWGG 1 cut(s) 46
TaaI ACNGT 8 cut(s) 289, 372, 402, 817, 878, 950, 1103, 1209
TaqI TCGA 4 cut(s) 28, 235, 669, 714
TasI AATT 8 cut(s) 57, 157, 175, 196, 297, 363, 803, 996
TatI WGTACW 4 cut(s) 230, 777, 916, 1195
TauI GCSGC 1 cut(s) 980
Tru1I TTAA 3 cut(s) 11, 140, 623
Tru9I TTAA 3 cut(s) 11, 140, 623
TscAI CASTG 5 cut(s) 317, 407, 820, 955, 1108
TseFI GTSAC 3 cut(s) 350, 811, 1161
TseI GCWGC 5 cut(s) 266, 356, 359, 758, 1081
Tsp45I GTSAC 3 cut(s) 350, 811, 1161
TspDTI ATGAA 7 cut(s) 28, 348, 539, 840, 1170, 1259, 1387
TspGWI ACGGA 3 cut(s) 906, 973, 1049
TspRI CASTG 5 cut(s) 317, 407, 820, 955, 1108
VpaK11BI GGWCC 1 cut(s) 1011
XapI RAATTY 2 cut(s) 57, 803
XbaI TCTAGA 1 cut(s) 110
XceI RCATGY 1 cut(s) 574
XhoI CTCGAG 1 cut(s) 668
XspI CTAG 2 cut(s) 111, 1001
ZrmI AGTACT 2 cut(s) 232, 1197
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.