FvH4_6g07390

Peptide chain release factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0014362
Physical Location & Seq
Forward (+)
4433295 .. 4436302
3008 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g07390.t1

Sequence Viewer

Length: 1467 bp
ATGTCATCTTTCTTGTTCAGAAAACGAGCCAGCTTTAGATGCTGGGTGATTCTGTACCCAAACCCTAAATCCAAACCTCAATTTGGTGATCTGATTAAACCCCCAAATTTTGAAATTCCCAGGATTTTGGATCATGGATCTTCTTCATTTTCACCCAAAACCTCAAGAACCCTATCTGGGTCTCTCCAGAACCTCAAAAACTCAATTGGTTCTGGTGAAAGTTTTGGGCTTCCTTCAACTTGTGGCTTGGTTCGATTTTATGGGACTCAGGCAGCTGAGGAGCCTTCAACCTCTGATGGGCTCACAGTTGATCGGATACTACATAGTGAGTGGTCGATTCACACAGAGGATGAGAGTGATTGGAAGAGTCACGCTGCTGCAATTGCCCAATCCATTCATCTTATTAAGAAACGTTTACAGTGGAAGAAGTTGATGGTCAGGTTGGATATGTTATCAGTGGAGCTGAATAAGCCAAACCTTTGGGATGATCCTGTACATGCTGGGAAGATAAGCCGCGAACATGGTTCACTGATGGGTAAAATGAAAGAAGTGAGGGCATTTGAGCAGGAATTACTTGAGCATGTGGATATGATAAAGCTTGCTCGGGAAGAGAATGACGCGGAGTTGGAATCGGAATCCATGAAGGCTTTGCTTGAGATGAGAAGAACTTCAAAGGAGAAAGAGACCCAAGCTTTATTAGCTGGGGAGCAGGATCCTTGCTCATGTTATATAGAGGTGCAATCGGGAGCTGGGGGTACAGAGAGCAATGACTGGGCAGCAATGGTCATGCAGATGTATAAAATGTGGGCTCAACGCCGTGGATATAAAATGACTGTGGTGGAGGAAATGCCTGGTGAGATTGCGGGAATCAAGCGGGCAACTATCAAAGTTGATGGTGAATATGCTTTTGGATATGCCAAAGCAGAAGTAGGAGTGCACAGGTTGGTACGTATCTCACCTTTTGACAGTAATAAGCGCAGACATACTTCATTTGCTGCTGTTGCTGTGACTCCAATCCTGGGAGATGTATCTGCTCATGTGCAAATTAATGAATCTGATTTGCGCATCGAGCGTTTTCGTTCTGGGGGATCTGGAGGCCAGCATGCTAATACAACTGATAGTGCTGTGAGGATTGTTCACATTCCCACTGGAATAACTGCCACTTGTCAAAATGAAAGATCACAGCATCAAAATAAGGCTTCAGCAATGGCTGTTATCCAGGCAAGAGTAGACCAGCTTGAAATGGCACGCCAGACTCAGAAGAATGCACAATATTCACAATCCCTTACCGATATAACCTGGGGAAGCCAGATTCGCAGCTACGTGCTCCAGCCATACCGCATGGTGAAAGATCTTAGAACCAACTATGAAGTCTCTGATCCTGATTCTGTCCTTGAAGGAGATCTAGACGGTTTTATCTTGAGTTATTTATCAGCTTCCTTGGATAAAGATGAAGATGACCGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PCRF RF-1

Protein Analysis

489

Amino Acids

54.75

Weight (kDa)

6.42

Isoelectric Point (pI)

46.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCRF PF03462 147 - 334 7.7e-51 PCRF domain
RF-1 PF00472 346 - 453 6e-36 RF-1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014362)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G56350
fragaria_vesca FvH4_6g07390
malus_domestica MD04G1193600.v1.1 MD12G1206300.v1.1
prunus_persica Prupe.6G313600_v2.0.a1 Prupe.6G313600_v2.0.a1
pyrus_communis pycom12g19420
rosa_chinensis RchiOBHm_Chr3g0456991
rosa_laevigata RLG00000025255
rosa_multiflora Rmu_co8479901.1_g000001
rosa_roxburghii Rroxscaffold_6G00422290
rosa_rugosa Rorug03G0018100
rosa_samantha Rh3AG077900 Rh3BG079900 Rh3CG079800 Rh3DG081600
rosa_wichuraiana Rw3G006690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1064
AccI GTMKAC 1 cut(s) 1230
AccII CGCG 2 cut(s) 516, 620
AciI CCGC 6 cut(s) 514, 620, 863, 874, 1339, 1462
AclI AACGTT 1 cut(s) 412
AclWI GGATC 7 cut(s) 138, 145, 482, 707, 720, 1096, 1373
AcsI RAATTY 2 cut(s) 106, 114
AcuI CTGAAG 1 cut(s) 1185
AfaI GTAC 4 cut(s) 56, 495, 757, 948
AfiI CCNNNNNNNGG 4 cut(s) 83, 177, 297, 1019
AgsI TTSAA 6 cut(s) 113, 237, 288, 672, 1241, 1397
AjnI CCWGG 5 cut(s) 119, 850, 1017, 1218, 1298
AjuI GAANNNNNNNTTGG 2 cut(s) 891, 923
Alw21I GWGCWC 2 cut(s) 939, 1329
Alw26I GTCTC 3 cut(s) 186, 677, 1378
Alw44I GTGCAC 1 cut(s) 935
AlwI GGATC 7 cut(s) 138, 145, 482, 707, 720, 1096, 1373
Ama87I CYCGRG 1 cut(s) 603
AoxI GGCC 1 cut(s) 1096
ApaLI GTGCAC 1 cut(s) 935
ApeKI GCWGC 6 cut(s) 272, 374, 377, 776, 995, 1317
ApoI RAATTY 2 cut(s) 106, 114
AseI ATTAAT 1 cut(s) 1047
Asp700I GAANNNNTTC 1 cut(s) 667
AspLEI GCGC 2 cut(s) 978, 1065
AsuHPI GGTGA 8 cut(s) 58, 98, 144, 227, 866, 908, 948, 1357
AvaI CYCGRG 1 cut(s) 603
BaeGI GKGCMC 1 cut(s) 939
BamHI GGATCC 1 cut(s) 712
BanII GRGCYC 2 cut(s) 303, 811
Bbv12I GWGCWC 2 cut(s) 939, 1329
BbvCI CCTCAGC 1 cut(s) 276
BbvI GCAGC 6 cut(s) 284, 361, 364, 788, 982, 1329
BccI CCATC 4 cut(s) 290, 427, 526, 887
BceAI ACGGC 1 cut(s) 801
BciT130I CCWGG 5 cut(s) 121, 852, 1019, 1220, 1300
BciVI GTATCC 1 cut(s) 309
BcoDI GTCTC 3 cut(s) 186, 677, 1378
BfaI CTAG 1 cut(s) 1406
BfuI GTATCC 1 cut(s) 309
BglII AGATCT 2 cut(s) 1351, 1402
BisI GCNGC 7 cut(s) 273, 375, 378, 514, 777, 996, 1318
BlsI GCNGC 7 cut(s) 274, 376, 379, 515, 778, 997, 1319
Bme1390I CCNGG 5 cut(s) 121, 852, 1019, 1220, 1300
BmeT110I CYCGRG 1 cut(s) 603
BmiI GGNNCC 2 cut(s) 282, 714
BmrFI CCNGG 5 cut(s) 121, 852, 1019, 1220, 1300
BmrI ACTGGG 1 cut(s) 781
BmsI GCATC 3 cut(s) 29, 1074, 1195
BmuI ACTGGG 1 cut(s) 781
BpmI CTGGAG 3 cut(s) 170, 1113, 1313
Bpu10I CCTNAGC 1 cut(s) 276
BpuEI CTTGAG 4 cut(s) 148, 596, 674, 1441
BsaAI YACGTR 2 cut(s) 950, 1324
BsaI GGTCTC 2 cut(s) 186, 677
BsaJI CCNNGG 5 cut(s) 119, 817, 1018, 1299, 1440
Bsc4I CCNNNNNNNGG 4 cut(s) 83, 177, 297, 1019
Bse1I ACTGG 2 cut(s) 776, 1153
Bse3DI GCAATG 3 cut(s) 772, 786, 1212
BseBI CCWGG 5 cut(s) 121, 852, 1019, 1220, 1300
BseDI CCNNGG 5 cut(s) 119, 817, 1018, 1299, 1440
BseGI GGATG 2 cut(s) 355, 490
BseLI CCNNNNNNNGG 4 cut(s) 83, 177, 297, 1019
BseMI GCAATG 3 cut(s) 772, 786, 1212
BseMII CTCAG 3 cut(s) 267, 281, 1271
BseNI ACTGG 2 cut(s) 776, 1153
BseRI GAGGAG 1 cut(s) 293
BseSI GKGCMC 1 cut(s) 939
BseXI GCAGC 6 cut(s) 284, 361, 364, 788, 982, 1329
BseYI CCCAGC 4 cut(s) 42, 500, 701, 749
Bsh1236I CGCG 2 cut(s) 516, 620
BshFI GGCC 1 cut(s) 1098
BsiHKAI GWGCWC 2 cut(s) 939, 1329
BsiHKCI CYCGRG 1 cut(s) 603
BslFI GGGAC 1 cut(s) 277
BslI CCNNNNNNNGG 4 cut(s) 83, 177, 297, 1019
BsmAI GTCTC 3 cut(s) 186, 677, 1378
BsmFI GGGAC 1 cut(s) 277
BsmI GAATGC 1 cut(s) 1270
BsnI GGCC 1 cut(s) 1098
Bso31I GGTCTC 2 cut(s) 186, 677
BsoBI CYCGRG 1 cut(s) 603
Bsp1286I GDGCHC 4 cut(s) 303, 811, 939, 1329
Bsp1407I TGTACA 1 cut(s) 493
BspACI CCGC 6 cut(s) 514, 620, 863, 874, 1339, 1462
BspANI GGCC 1 cut(s) 1098
BspCNI CTCAG 3 cut(s) 268, 280, 1270
BspFNI CGCG 2 cut(s) 516, 620
BspLI GGNNCC 2 cut(s) 282, 714
BspPI GGATC 7 cut(s) 138, 145, 482, 707, 720, 1096, 1373
BspTNI GGTCTC 2 cut(s) 186, 677
BsrDI GCAATG 3 cut(s) 772, 786, 1212
BsrGI TGTACA 1 cut(s) 493
BsrI ACTGG 2 cut(s) 776, 1153
BssECI CCNNGG 5 cut(s) 119, 817, 1018, 1299, 1440
BssT1I CCWWGG 1 cut(s) 1440
Bst2UI CCWGG 5 cut(s) 121, 852, 1019, 1220, 1300
Bst4CI ACNGT 5 cut(s) 307, 420, 835, 968, 1412
Bst6I CTCTTC 2 cut(s) 359, 603
BstAUI TGTACA 1 cut(s) 493
BstBAI YACGTR 2 cut(s) 950, 1324
BstC8I GCNNGC 6 cut(s) 31, 600, 876, 1100, 1104, 1249
BstDEI CTNAG 4 cut(s) 267, 276, 1257, 1355
BstDSI CCRYGG 1 cut(s) 817
BstF5I GGATG 2 cut(s) 355, 490
BstFNI CGCG 2 cut(s) 516, 620
BstHHI GCGC 2 cut(s) 978, 1065
BstMAI GTCTC 3 cut(s) 186, 677, 1378
BstMWI GCNNNNNNNGC 7 cut(s) 39, 383, 469, 698, 1001, 1069, 1314
BstNI CCWGG 5 cut(s) 121, 852, 1019, 1220, 1300
BstNSI RCATGY 3 cut(s) 500, 584, 1106
BstSCI CCNGG 5 cut(s) 119, 850, 1017, 1218, 1298
BstSLI GKGCMC 1 cut(s) 939
BstSNI TACGTA 1 cut(s) 950
BstUI CGCG 2 cut(s) 516, 620
BstV1I GCAGC 6 cut(s) 284, 361, 364, 788, 982, 1329
BstX2I RGATCY 5 cut(s) 137, 712, 1088, 1351, 1402
BstXI CCANNNNNNTGG 2 cut(s) 127, 480
BstYI RGATCY 5 cut(s) 137, 712, 1088, 1351, 1402
BsuI GTATCC 1 cut(s) 309
BsuRI GGCC 1 cut(s) 1098
BtgI CCRYGG 1 cut(s) 817
BtsCI GGATG 2 cut(s) 355, 490
BtsIMutI CAGTG 4 cut(s) 425, 462, 527, 1146
Cac8I GCNNGC 6 cut(s) 31, 600, 876, 1100, 1104, 1249
CfoI GCGC 2 cut(s) 978, 1065
CseI GACGC 1 cut(s) 626
Csp6I GTAC 4 cut(s) 55, 494, 756, 947
CviQI GTAC 4 cut(s) 55, 494, 756, 947
DdeI CTNAG 4 cut(s) 267, 276, 1257, 1355
Eam1104I CTCTTC 2 cut(s) 359, 603
EarI CTCTTC 2 cut(s) 359, 603
Eco105I TACGTA 1 cut(s) 950
Eco130I CCWWGG 1 cut(s) 1440
Eco24I GRGCYC 2 cut(s) 303, 811
Eco31I GGTCTC 2 cut(s) 186, 677
Eco57I CTGAAG 1 cut(s) 1185
Eco88I CYCGRG 1 cut(s) 603
EcoRII CCWGG 5 cut(s) 119, 850, 1017, 1218, 1298
EcoT14I CCWWGG 1 cut(s) 1440
EcoT38I GRGCYC 2 cut(s) 303, 811
ErhI CCWWGG 1 cut(s) 1440
FaqI GGGAC 1 cut(s) 277
FauI CCCGC 2 cut(s) 856, 867
FblI GTMKAC 1 cut(s) 1230
Fnu4HI GCNGC 7 cut(s) 273, 375, 378, 514, 777, 996, 1318
FokI GGATG 2 cut(s) 362, 497
FriOI GRGCYC 2 cut(s) 303, 811
Fsp4HI GCNGC 7 cut(s) 273, 375, 378, 514, 777, 996, 1318
FspBI CTAG 1 cut(s) 1406
FspI TGCGCA 1 cut(s) 1064
GlaI GCGC 2 cut(s) 977, 1064
GluI GCNGC 7 cut(s) 273, 375, 378, 514, 777, 996, 1318
GsaI CCCAGC 4 cut(s) 46, 504, 705, 753
GsuI CTGGAG 3 cut(s) 170, 1113, 1313
HaeIII GGCC 1 cut(s) 1098
HgaI GACGC 1 cut(s) 626
HhaI GCGC 2 cut(s) 978, 1065
Hin6I GCGC 2 cut(s) 976, 1063
HinP1I GCGC 2 cut(s) 976, 1063
HindIII AAGCTT 2 cut(s) 596, 690
HphI GGTGA 8 cut(s) 58, 98, 144, 227, 866, 908, 948, 1357
Hpy166II GTNNAC 5 cut(s) 416, 527, 937, 1138, 1231
Hpy188I TCNGA 8 cut(s) 20, 93, 295, 315, 634, 1057, 1260, 1378
Hpy188III TCNNGA 8 cut(s) 165, 187, 605, 744, 1092, 1382, 1406, 1420
Hpy8I GTNNAC 5 cut(s) 416, 527, 937, 1138, 1231
HpyAV CCTTC 4 cut(s) 243, 294, 637, 1391
HpyCH4III ACNGT 5 cut(s) 307, 420, 835, 968, 1412
HpyCH4IV ACGT 3 cut(s) 412, 949, 1323
HpyCH4V TGCA 6 cut(s) 380, 739, 790, 937, 1042, 1268
HpyF10VI GCNNNNNNNGC 7 cut(s) 39, 383, 469, 698, 1001, 1069, 1314
HpyF3I CTNAG 4 cut(s) 267, 276, 1257, 1355
HpySE526I ACGT 3 cut(s) 412, 949, 1323
HspAI GCGC 2 cut(s) 976, 1063
LmnI GCTCC 5 cut(s) 280, 460, 706, 746, 1332
Lsp1109I GCAGC 6 cut(s) 284, 361, 364, 788, 982, 1329
LweI GCATC 3 cut(s) 29, 1074, 1195
MaeI CTAG 1 cut(s) 1406
MaeII ACGT 3 cut(s) 412, 949, 1323
MaeIII GTNAC 2 cut(s) 368, 1006
MboII GAAGA 9 cut(s) 132, 135, 376, 436, 517, 620, 675, 1273, 1466
MfeI CAATTG 2 cut(s) 204, 381
MflI RGATCY 5 cut(s) 137, 712, 1088, 1351, 1402
MhlI GDGCHC 4 cut(s) 303, 811, 939, 1329
MluCI AATT 7 cut(s) 80, 106, 114, 204, 381, 569, 1044
MlyI GAGTC 4 cut(s) 259, 376, 1003, 1249
MmeI TCCRAC 2 cut(s) 423, 606
MroXI GAANNNNTTC 1 cut(s) 667
MseI TTAA 3 cut(s) 96, 405, 1047
MslI CAYNNNNRTG 1 cut(s) 791
MspA1I CMGCKG 1 cut(s) 275
MspR9I CCNGG 5 cut(s) 121, 852, 1019, 1220, 1300
MunI CAATTG 2 cut(s) 204, 381
Mva1269I GAATGC 1 cut(s) 1270
MvaI CCWGG 5 cut(s) 121, 852, 1019, 1220, 1300
MvnI CGCG 2 cut(s) 516, 620
MwoI GCNNNNNNNGC 7 cut(s) 39, 383, 469, 698, 1001, 1069, 1314
NlaIV GGNNCC 2 cut(s) 282, 714
NmuCI GTSAC 2 cut(s) 368, 1006
NsbI TGCGCA 1 cut(s) 1064
NspI RCATGY 3 cut(s) 500, 584, 1106
PaeI GCATGC 1 cut(s) 1106
PctI GAATGC 1 cut(s) 1270
PdmI GAANNNNTTC 1 cut(s) 667
PfeI GAWTC 8 cut(s) 49, 337, 629, 635, 867, 1052, 1312, 1385
PkrI GCNGC 7 cut(s) 274, 376, 379, 515, 778, 997, 1319
PleI GAGTC 4 cut(s) 259, 375, 1003, 1249
PpsI GAGTC 4 cut(s) 259, 375, 1003, 1249
Ppu21I YACGTR 2 cut(s) 950, 1324
PshBI ATTAAT 1 cut(s) 1047
Psp1406I AACGTT 1 cut(s) 412
Psp6I CCWGG 5 cut(s) 119, 850, 1017, 1218, 1298
PspFI CCCAGC 4 cut(s) 42, 500, 701, 749
PspGI CCWGG 5 cut(s) 119, 850, 1017, 1218, 1298
PspN4I GGNNCC 2 cut(s) 282, 714
PsuI RGATCY 5 cut(s) 137, 712, 1088, 1351, 1402
PvuII CAGCTG 1 cut(s) 275
RsaI GTAC 4 cut(s) 56, 495, 757, 948
RsaNI GTAC 4 cut(s) 55, 494, 756, 947
RseI CAYNNNNRTG 1 cut(s) 791
SaqAI TTAA 3 cut(s) 96, 405, 1047
SatI GCNGC 7 cut(s) 273, 375, 378, 514, 777, 996, 1318
SchI GAGTC 4 cut(s) 259, 376, 1003, 1249
ScrFI CCNGG 5 cut(s) 121, 852, 1019, 1220, 1300
SduI GDGCHC 4 cut(s) 303, 811, 939, 1329
SfaNI GCATC 3 cut(s) 29, 1074, 1195
SmiMI CAYNNNNRTG 1 cut(s) 791
SmlI CTYRAG 4 cut(s) 163, 575, 653, 1420
SmoI CTYRAG 4 cut(s) 163, 575, 653, 1420
SnaBI TACGTA 1 cut(s) 950
SphI GCATGC 1 cut(s) 1106
Sse9I AATT 7 cut(s) 80, 106, 114, 204, 381, 569, 1044
SsiI CCGC 6 cut(s) 514, 620, 863, 874, 1339, 1462
SspI AATATT 1 cut(s) 1274
SspMI CTAG 1 cut(s) 1406
StyD4I CCNGG 5 cut(s) 119, 850, 1017, 1218, 1298
StyI CCWWGG 1 cut(s) 1440
TaaI ACNGT 5 cut(s) 307, 420, 835, 968, 1412
TaiI ACGT 3 cut(s) 415, 952, 1326
TaqI TCGA 3 cut(s) 253, 335, 1068
TasI AATT 7 cut(s) 80, 106, 114, 204, 381, 569, 1044
TatI WGTACW 1 cut(s) 493
TauI GCSGC 1 cut(s) 516
TfiI GAWTC 8 cut(s) 49, 337, 629, 635, 867, 1052, 1312, 1385
Tru1I TTAA 3 cut(s) 96, 405, 1047
Tru9I TTAA 3 cut(s) 96, 405, 1047
TscAI CASTG 4 cut(s) 425, 462, 534, 1153
TseFI GTSAC 2 cut(s) 368, 1006
TseI GCWGC 6 cut(s) 272, 374, 377, 776, 995, 1317
Tsp45I GTSAC 2 cut(s) 368, 1006
TspDTI ATGAA 9 cut(s) 135, 386, 557, 656, 978, 1065, 1188, 1383, 1467
TspRI CASTG 4 cut(s) 425, 462, 534, 1153
VneI GTGCAC 1 cut(s) 935
VspI ATTAAT 1 cut(s) 1047
XapI RAATTY 2 cut(s) 106, 114
XbaI TCTAGA 1 cut(s) 1405
XceI RCATGY 3 cut(s) 500, 584, 1106
XmiI GTMKAC 1 cut(s) 1230
XmnI GAANNNNTTC 1 cut(s) 667
XspI CTAG 1 cut(s) 1406
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.