MD12G1206300.v1.1

Peptide chain release factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0014362
Physical Location & Seq
Reverse (-)
28690493 .. 28694049
3557 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1206300.v1.1.491

Sequence Viewer

Length: 1629 bp
ATGTCATCTTTCATACTCAGAAGGAGAGCAAGTAGTAGTGGATCCTGCCAGCTTCTCTGCCCAAACCCAGAAGCCAAAAGCGGCTTTTTCCCCAATCTGCTTTTCTCTACTCTGTCTGAATCCCAACCCGGAAATCTAAATCAACCCTTAAACCCTCAAAATGTACTGCATCCAATTGGAAAACATAGGATTCTGGATTCTGGGTTCTCTTCATTTTCTCCCAATACCTCAAAAACCCCATCCGGATCCCTCCAAAATCTCACCTTTTCGAGTAGTATCTTCGTTTCGGGCTCCAAGCCTCTTGCTTTAAGCTCTGGAAATGCTGCTTCAGGGAAAAATATCAGTGGGTGTGGTGTATTGGGGCTGCCAACGGTGAAAAGTTTGTATTCGGGTTGCGGCAGGTTCGGGTTGGTTTGCTTGTTTGGTACTCAGGCTGCTGTGGAGCCGTCGACGTGTGACGGGCTGACGGTTGATCGAATCATTGCTAGTGAGTGGCCGATTCTGGATGAGGATGAGAGTGATTGGAAGAGTCATGCTGCTGCAATCGCACAGTCCATTCATCTTATTAAGAAACGTTTGCAGTGGAAGAAGCTGCTGGTTAGGTTGGATATGTTATCAGCGGAGGTGAATAAGCTAGACCTGTGGAATGATCCTGTACACGCAGGGAAGATAAGCCGCGAGCATGGGTCTCTTTTGGGTAAAATGAAAGAAGTGCAGGCATTTGAGCGGGAATTGCTTGAGCATGTAGACATGGTAAAGCTTGCCCGTGAAGAGAATGACTCAGAGCTGGAATCGGAATCCATGAACGCTTTGCTTAATATGAGAAGAACTTCAAAGGAGAAAGAGATTGAAGCTTTGTTAGCTGGGGAGCAGGATCCTTGCTCGTGTTACATTGAGGTTCAAGCAGGAGCTGGGGGTACAGAGAGCAATGACTGGGCAGCAATGGTCATGCAGATGTATAAAATGTGGGCTCAACGACGTGGATATAACATGACTGTGGTGGATGAAATGCCCGGTGAGATTGCGGGAATCAAGCGGGCAACAATCAAAGTTGATGGTGGGTATGCTTTTGGATATGCCAAAGCAGAAGTAGGAGTGCACAGGTTGGTACGGATCTCACCTTTTGACAGTAACAAGCGCAGACACACTTCATTTGCTGCTGTTGCTGTGACTCCAATCCTGGGAGATGCATCTGAGCATGTACAAATTAATGAATCTGATCTGCGCATTGAGCGATTTCGTTCTGGGGGAGCTGGAGGCCAGCATGCTAATACAACTGATAGTGCTGTGAGGATTGTTCACGTTCCTACAGGAATTACTGCTACTTGTCAGAATGAGAGATCACAACATCAGAATAAGGCTTCAGCAATGGCGGTTATTCAGTCACGAGTAGACCAGCTTGAGATGGCACGCCAGAATAAGATGAATGCACAGTATTCACAATCTCTTACAGACATAACCTGGGGTAGCCAGATTCGCAGCTACGTACTCCAGCCATACCGGATGGTGAAAGATCTTCGGACAAACTATGAAGTCTCAGATCCTGATTCTGTCCTTGAGGGAGATCTAGATGGCTTCATCCTGAGCTATTTATCAGCGTGCTTGGATAAAGACGAAGATGACAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PCRF RF-1

Protein Analysis

543

Amino Acids

59.47

Weight (kDa)

6.05

Isoelectric Point (pI)

51.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCRF PF03462 201 - 388 4.3e-49 PCRF domain
RF-1 PF00472 400 - 507 1.1e-35 RF-1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014362)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G56350
fragaria_vesca FvH4_6g07390
malus_domestica MD04G1193600.v1.1 MD12G1206300.v1.1
prunus_persica Prupe.6G313600_v2.0.a1 Prupe.6G313600_v2.0.a1
pyrus_communis pycom12g19420
rosa_chinensis RchiOBHm_Chr3g0456991
rosa_laevigata RLG00000025255
rosa_multiflora Rmu_co8479901.1_g000001
rosa_roxburghii Rroxscaffold_6G00422290
rosa_rugosa Rorug03G0018100
rosa_samantha Rh3AG077900 Rh3BG079900 Rh3CG079800 Rh3DG081600
rosa_wichuraiana Rw3G006690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1226
Acc36I ACCTGC 1 cut(s) 390
AccBSI CCGCTC 1 cut(s) 727
AccI GTMKAC 3 cut(s) 449, 747, 1392
AccII CGCG 1 cut(s) 678
AccIII TCCGGA 1 cut(s) 242
AciI CCGC 8 cut(s) 81, 396, 620, 676, 727, 1025, 1036, 1373
AclI AACGTT 1 cut(s) 574
AclWI GGATC 9 cut(s) 36, 49, 240, 253, 644, 869, 882, 1121, 1535
AcoI YGGCCR 1 cut(s) 494
AcuI CTGAAG 2 cut(s) 312, 1347
AfaI GTAC 7 cut(s) 165, 427, 657, 919, 1110, 1203, 1488
AfiI CCNNNNNNNGG 1 cut(s) 1181
AflIII ACRYGT 1 cut(s) 452
AgsI TTSAA 3 cut(s) 834, 851, 902
AjiI CACGTC 2 cut(s) 453, 980
AjnI CCWGG 2 cut(s) 1179, 1460
AjuI GAANNNNNNNTTGG 2 cut(s) 86, 118
AloI GAACNNNNNNTCC 2 cut(s) 188, 220
Alw21I GWGCWC 1 cut(s) 1101
Alw26I GTCTC 2 cut(s) 693, 1540
Alw44I GTGCAC 1 cut(s) 1097
AlwI GGATC 9 cut(s) 36, 49, 240, 253, 644, 869, 882, 1121, 1535
AlwNI CAGNNNCTG 2 cut(s) 911, 1544
Aor13HI TCCGGA 1 cut(s) 242
AoxI GGCC 2 cut(s) 494, 1258
ApaLI GTGCAC 1 cut(s) 1097
ApeKI GCWGC 9 cut(s) 323, 364, 434, 536, 539, 592, 938, 1157, 1479
AseI ATTAAT 1 cut(s) 1209
Asp700I GAANNNNTTC 1 cut(s) 829
AspLEI GCGC 2 cut(s) 1140, 1227
AsuC2I CCSGG 2 cut(s) 129, 1014
AsuHPI GGTGA 6 cut(s) 253, 385, 637, 1028, 1110, 1519
BaeGI GKGCMC 1 cut(s) 1101
BamHI GGATCC 3 cut(s) 41, 245, 874
BanII GRGCYC 2 cut(s) 293, 973
BauI CACGAG 2 cut(s) 883, 1386
Bbv12I GWGCWC 1 cut(s) 1101
BbvI GCAGC 9 cut(s) 310, 351, 421, 523, 526, 579, 950, 1144, 1491
BccI CCATC 5 cut(s) 247, 1049, 1399, 1498, 1565
BceAI ACGGC 1 cut(s) 430
BciT130I CCWGG 2 cut(s) 1181, 1462
BcnI CCSGG 2 cut(s) 129, 1014
BcoDI GTCTC 2 cut(s) 693, 1540
BfaI CTAG 3 cut(s) 486, 635, 1568
BfmI CTRYAG 1 cut(s) 1308
BfuAI ACCTGC 1 cut(s) 390
BglII AGATCT 2 cut(s) 1513, 1564
Bme1390I CCNGG 4 cut(s) 129, 1014, 1181, 1462
BmgBI CACGTC 2 cut(s) 453, 980
BmiI GGNNCC 5 cut(s) 43, 247, 292, 444, 876
BmrFI CCNGG 4 cut(s) 129, 1014, 1181, 1462
BmrI ACTGGG 1 cut(s) 943
BmsI GCATC 3 cut(s) 178, 1177, 1199
BmuI ACTGGG 1 cut(s) 943
BplI GAGNNNNNCTC 2 cut(s) 764, 796
BpmI CTGGAG 2 cut(s) 1275, 1475
Bpu10I CCTNAGC 1 cut(s) 1583
BpuEI CTTGAG 3 cut(s) 758, 1421, 1577
BpuMI CCSGG 2 cut(s) 129, 1014
BsaAI YACGTR 1 cut(s) 1486
BsaI GGTCTC 1 cut(s) 693
BsaJI CCNNGG 2 cut(s) 1180, 1461
BsaWI WCCGGW 2 cut(s) 242, 1500
BsaXI ACNNNNNCTCC 2 cut(s) 16, 46
Bsc4I CCNNNNNNNGG 1 cut(s) 1181
Bse1I ACTGG 1 cut(s) 938
Bse3DI GCAATG 4 cut(s) 480, 934, 948, 1374
BseAI TCCGGA 1 cut(s) 242
BseBI CCWGG 2 cut(s) 1181, 1462
BseDI CCNNGG 2 cut(s) 1180, 1461
BseGI GGATG 7 cut(s) 169, 239, 511, 517, 1009, 1509, 1578
BseLI CCNNNNNNNGG 1 cut(s) 1181
BseMI GCAATG 4 cut(s) 480, 934, 948, 1374
BseMII CTCAG 6 cut(s) 31, 443, 795, 1185, 1551, 1574
BseNI ACTGG 1 cut(s) 938
BseSI GKGCMC 1 cut(s) 1101
BseXI GCAGC 9 cut(s) 310, 351, 421, 523, 526, 579, 950, 1144, 1491
BseYI CCCAGC 2 cut(s) 863, 911
BsgI GTGCAG 1 cut(s) 734
Bsh1236I CGCG 1 cut(s) 678
BshFI GGCC 2 cut(s) 496, 1260
BsiHKAI GWGCWC 1 cut(s) 1101
BsiSI CCGG 4 cut(s) 129, 243, 1014, 1501
BslI CCNNNNNNNGG 1 cut(s) 1181
BsmAI GTCTC 2 cut(s) 693, 1540
BsmI GAATGC 1 cut(s) 1432
BsnI GGCC 2 cut(s) 496, 1260
Bso31I GGTCTC 1 cut(s) 693
Bsp1286I GDGCHC 3 cut(s) 293, 973, 1101
Bsp13I TCCGGA 1 cut(s) 242
Bsp1407I TGTACA 2 cut(s) 655, 1201
BspACI CCGC 8 cut(s) 81, 396, 620, 676, 727, 1025, 1036, 1373
BspANI GGCC 2 cut(s) 496, 1260
BspCNI CTCAG 6 cut(s) 30, 442, 794, 1186, 1550, 1575
BspEI TCCGGA 1 cut(s) 242
BspFNI CGCG 1 cut(s) 678
BspLI GGNNCC 5 cut(s) 43, 247, 292, 444, 876
BspMI ACCTGC 1 cut(s) 390
BspPI GGATC 9 cut(s) 36, 49, 240, 253, 644, 869, 882, 1121, 1535
BspTNI GGTCTC 1 cut(s) 693
BsrBI CCGCTC 1 cut(s) 727
BsrDI GCAATG 4 cut(s) 480, 934, 948, 1374
BsrGI TGTACA 2 cut(s) 655, 1201
BsrI ACTGG 1 cut(s) 938
BssECI CCNNGG 2 cut(s) 1180, 1461
BssSI CACGAG 2 cut(s) 883, 1386
Bst2BI CACGAG 2 cut(s) 883, 1386
Bst2UI CCWGG 2 cut(s) 1181, 1462
Bst4CI ACNGT 6 cut(s) 373, 469, 552, 997, 1130, 1434
Bst6I CTCTTC 3 cut(s) 214, 521, 765
BstAUI TGTACA 2 cut(s) 655, 1201
BstBAI YACGTR 1 cut(s) 1486
BstC8I GCNNGC 9 cut(s) 50, 680, 717, 762, 1038, 1262, 1266, 1411, 1600
BstDEI CTNAG 6 cut(s) 17, 429, 781, 1194, 1537, 1583
BstF5I GGATG 7 cut(s) 169, 239, 511, 517, 1009, 1509, 1578
BstFNI CGCG 1 cut(s) 678
BstHHI GCGC 2 cut(s) 1140, 1227
BstMAI GTCTC 2 cut(s) 693, 1540
BstMWI GCNNNNNNNGC 6 cut(s) 545, 733, 860, 1163, 1231, 1476
BstNI CCWGG 2 cut(s) 1181, 1462
BstNSI RCATGY 3 cut(s) 746, 1202, 1268
BstSCI CCNGG 4 cut(s) 127, 1012, 1179, 1460
BstSFI CTRYAG 1 cut(s) 1308
BstSLI GKGCMC 1 cut(s) 1101
BstSNI TACGTA 1 cut(s) 1486
BstUI CGCG 1 cut(s) 678
BstV1I GCAGC 9 cut(s) 310, 351, 421, 523, 526, 579, 950, 1144, 1491
BstX2I RGATCY 7 cut(s) 41, 245, 874, 1113, 1513, 1540, 1564
BstYI RGATCY 7 cut(s) 41, 245, 874, 1113, 1513, 1540, 1564
BsuRI GGCC 2 cut(s) 496, 1260
BtrI CACGTC 2 cut(s) 453, 980
BtsCI GGATG 7 cut(s) 169, 239, 511, 517, 1009, 1509, 1578
BtsI GCAGTG 1 cut(s) 587
BtsIMutI CAGTG 2 cut(s) 349, 587
BveI ACCTGC 1 cut(s) 390
Cac8I GCNNGC 9 cut(s) 50, 680, 717, 762, 1038, 1262, 1266, 1411, 1600
CaiI CAGNNNCTG 2 cut(s) 911, 1544
CfoI GCGC 2 cut(s) 1140, 1227
Csp6I GTAC 7 cut(s) 164, 426, 656, 918, 1109, 1202, 1487
CviAII CATG 9 cut(s) 533, 683, 743, 751, 802, 949, 991, 1199, 1265
CviQI GTAC 7 cut(s) 164, 426, 656, 918, 1109, 1202, 1487
DdeI CTNAG 6 cut(s) 17, 429, 781, 1194, 1537, 1583
EaeI YGGCCR 1 cut(s) 494
Eam1104I CTCTTC 3 cut(s) 214, 521, 765
EarI CTCTTC 3 cut(s) 214, 521, 765
Eco105I TACGTA 1 cut(s) 1486
Eco24I GRGCYC 2 cut(s) 293, 973
Eco31I GGTCTC 1 cut(s) 693
Eco57I CTGAAG 2 cut(s) 312, 1347
EcoRII CCWGG 2 cut(s) 1179, 1460
EcoT22I ATGCAT 1 cut(s) 1192
EcoT38I GRGCYC 2 cut(s) 293, 973
FaeI CATG 9 cut(s) 536, 686, 746, 754, 805, 952, 994, 1202, 1268
FatI CATG 9 cut(s) 532, 682, 742, 750, 801, 948, 990, 1198, 1264
FauI CCCGC 3 cut(s) 720, 1018, 1029
FblI GTMKAC 3 cut(s) 449, 747, 1392
FokI GGATG 7 cut(s) 156, 226, 518, 524, 1016, 1516, 1565
FriOI GRGCYC 2 cut(s) 293, 973
FspBI CTAG 3 cut(s) 486, 635, 1568
FspI TGCGCA 1 cut(s) 1226
GlaI GCGC 2 cut(s) 1139, 1226
GsaI CCCAGC 2 cut(s) 867, 915
GsuI CTGGAG 2 cut(s) 1275, 1475
HaeIII GGCC 2 cut(s) 496, 1260
HapII CCGG 4 cut(s) 129, 243, 1014, 1501
HhaI GCGC 2 cut(s) 1140, 1227
Hin1II CATG 9 cut(s) 536, 686, 746, 754, 805, 952, 994, 1202, 1268
Hin6I GCGC 2 cut(s) 1138, 1225
HinP1I GCGC 2 cut(s) 1138, 1225
HincII GTYRAC 1 cut(s) 450
HindII GTYRAC 1 cut(s) 450
HindIII AAGCTT 2 cut(s) 758, 852
HpaII CCGG 4 cut(s) 129, 243, 1014, 1501
HphI GGTGA 6 cut(s) 253, 385, 637, 1028, 1110, 1519
Hpy166II GTNNAC 6 cut(s) 450, 658, 748, 1099, 1300, 1393
Hpy188III TCNNGA 8 cut(s) 194, 243, 315, 503, 1386, 1544, 1568, 1582
Hpy8I GTNNAC 6 cut(s) 450, 658, 748, 1099, 1300, 1393
Hpy99I CGWCG 3 cut(s) 451, 454, 981
HpyAV CCTTC 1 cut(s) 15
HpyCH4III ACNGT 6 cut(s) 373, 469, 552, 997, 1130, 1434
HpyCH4IV ACGT 5 cut(s) 452, 574, 979, 1302, 1485
HpyCH4V TGCA 8 cut(s) 169, 542, 580, 715, 952, 1099, 1190, 1430
HpyF10VI GCNNNNNNNGC 6 cut(s) 545, 733, 860, 1163, 1231, 1476
HpyF3I CTNAG 6 cut(s) 17, 429, 781, 1194, 1537, 1583
HpySE526I ACGT 5 cut(s) 452, 574, 979, 1302, 1485
Hsp92II CATG 9 cut(s) 536, 686, 746, 754, 805, 952, 994, 1202, 1268
HspAI GCGC 2 cut(s) 1138, 1225
Kpn2I TCCGGA 1 cut(s) 242
LmnI GCTCC 5 cut(s) 296, 442, 868, 908, 1250
Lsp1109I GCAGC 9 cut(s) 310, 351, 421, 523, 526, 579, 950, 1144, 1491
LweI GCATC 3 cut(s) 178, 1177, 1199
MaeI CTAG 3 cut(s) 486, 635, 1568
MaeII ACGT 5 cut(s) 452, 574, 979, 1302, 1485
MaeIII GTNAC 5 cut(s) 455, 887, 1130, 1168, 1383
MbiI CCGCTC 1 cut(s) 727
MboII GAAGA 9 cut(s) 201, 271, 538, 598, 679, 782, 837, 1508, 1628
MfeI CAATTG 1 cut(s) 174
MflI RGATCY 7 cut(s) 41, 245, 874, 1113, 1513, 1540, 1564
MhlI GDGCHC 3 cut(s) 293, 973, 1101
MluCI AATT 4 cut(s) 174, 731, 1206, 1314
MlyI GAGTC 3 cut(s) 538, 773, 1165
MmeI TCCRAC 1 cut(s) 585
Mph1103I ATGCAT 1 cut(s) 1192
MroI TCCGGA 1 cut(s) 242
MroXI GAANNNNTTC 1 cut(s) 829
MseI TTAA 5 cut(s) 149, 308, 567, 816, 1209
MslI CAYNNNNRTG 2 cut(s) 953, 995
MspA1I CMGCKG 1 cut(s) 620
MspI CCGG 4 cut(s) 129, 243, 1014, 1501
MspR9I CCNGG 4 cut(s) 129, 1014, 1181, 1462
MunI CAATTG 1 cut(s) 174
Mva1269I GAATGC 1 cut(s) 1432
MvaI CCWGG 2 cut(s) 1181, 1462
MvnI CGCG 1 cut(s) 678
MwoI GCNNNNNNNGC 6 cut(s) 545, 733, 860, 1163, 1231, 1476
NciI CCSGG 2 cut(s) 129, 1014
NlaIII CATG 9 cut(s) 536, 686, 746, 754, 805, 952, 994, 1202, 1268
NlaIV GGNNCC 5 cut(s) 43, 247, 292, 444, 876
NmuCI GTSAC 3 cut(s) 455, 1168, 1383
NsbI TGCGCA 1 cut(s) 1226
NsiI ATGCAT 1 cut(s) 1192
NspI RCATGY 3 cut(s) 746, 1202, 1268
PaeI GCATGC 1 cut(s) 1268
PctI GAATGC 1 cut(s) 1432
PdmI GAANNNNTTC 1 cut(s) 829
PleI GAGTC 3 cut(s) 537, 773, 1165
PpsI GAGTC 3 cut(s) 537, 773, 1165
Ppu21I YACGTR 1 cut(s) 1486
PshBI ATTAAT 1 cut(s) 1209
Psp1406I AACGTT 1 cut(s) 574
Psp6I CCWGG 2 cut(s) 1179, 1460
PspFI CCCAGC 2 cut(s) 863, 911
PspGI CCWGG 2 cut(s) 1179, 1460
PspN4I GGNNCC 5 cut(s) 43, 247, 292, 444, 876
PstNI CAGNNNCTG 2 cut(s) 911, 1544
PsuI RGATCY 7 cut(s) 41, 245, 874, 1113, 1513, 1540, 1564
RsaI GTAC 7 cut(s) 165, 427, 657, 919, 1110, 1203, 1488
RsaNI GTAC 7 cut(s) 164, 426, 656, 918, 1109, 1202, 1487
RseI CAYNNNNRTG 2 cut(s) 953, 995
SalI GTCGAC 1 cut(s) 448
SaqAI TTAA 5 cut(s) 149, 308, 567, 816, 1209
SchI GAGTC 3 cut(s) 538, 773, 1165
ScrFI CCNGG 4 cut(s) 129, 1014, 1181, 1462
SduI GDGCHC 3 cut(s) 293, 973, 1101
SfaNI GCATC 3 cut(s) 178, 1177, 1199
SfcI CTRYAG 1 cut(s) 1308
SgrDI CGTCGACG 1 cut(s) 448
SmiMI CAYNNNNRTG 2 cut(s) 953, 995
SmlI CTYRAG 3 cut(s) 737, 1400, 1556
SmoI CTYRAG 3 cut(s) 737, 1400, 1556
SnaBI TACGTA 1 cut(s) 1486
SphI GCATGC 1 cut(s) 1268
Sse9I AATT 4 cut(s) 174, 731, 1206, 1314
SsiI CCGC 8 cut(s) 81, 396, 620, 676, 727, 1025, 1036, 1373
SspMI CTAG 3 cut(s) 486, 635, 1568
StyD4I CCNGG 4 cut(s) 127, 1012, 1179, 1460
TaaI ACNGT 6 cut(s) 373, 469, 552, 997, 1130, 1434
TaiI ACGT 5 cut(s) 455, 577, 982, 1305, 1488
TaqI TCGA 3 cut(s) 269, 449, 475
TasI AATT 4 cut(s) 174, 731, 1206, 1314
TatI WGTACW 3 cut(s) 163, 655, 1201
TauI GCSGC 3 cut(s) 84, 399, 678
Tru1I TTAA 5 cut(s) 149, 308, 567, 816, 1209
Tru9I TTAA 5 cut(s) 149, 308, 567, 816, 1209
TscAI CASTG 2 cut(s) 349, 587
TseFI GTSAC 3 cut(s) 455, 1168, 1383
TseI GCWGC 9 cut(s) 323, 364, 434, 536, 539, 592, 938, 1157, 1479
Tsp45I GTSAC 3 cut(s) 455, 1168, 1383
TspGWI ACGGA 1 cut(s) 1126
TspRI CASTG 2 cut(s) 349, 587
VneI GTGCAC 1 cut(s) 1097
VspI ATTAAT 1 cut(s) 1209
XbaI TCTAGA 1 cut(s) 1567
XceI RCATGY 3 cut(s) 746, 1202, 1268
XmiI GTMKAC 3 cut(s) 449, 747, 1392
XmnI GAANNNNTTC 1 cut(s) 829
XspI CTAG 3 cut(s) 486, 635, 1568
Zsp2I ATGCAT 1 cut(s) 1192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.