Rorug03G0018100

Peptide chain release factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0014362
Physical Location & Seq
Forward (+)
1438821 .. 1440843
2023 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0018100.1

Sequence Viewer

Length: 1893 bp
ATGGAGCCTCTGCTTTTCTTGCTTATGATTGTGTTCCCCAGTATTGTTAAACCAATTAGCAAAGCAGCCAATGATCAGGTCTGCCCAGTTCAAAGGTGCAGGCATGATGGTCCTGATATCAAGTTTCCATTCCGTCTACAACACTATCCCCTTCACTGTGGCCATCCAGAGTTTGAGGTTTCCTGCAGCAGCTTCACCAACATGACCATGATGAAGCTTTCCTCATCATCAGGATTCTTTCCAATACAAACAATCGATTACGAGAGTCAACAGTTTCGTGTGTATGATCAGGATGGTTGCCTCCCGAGACGCCTTCTTAATTTTACCGTCTCCTCCTCGCTCTTTCAACCAGCAGTTGTGAGTGAAGATTCCGTTTGCGGTTACGATTACATGAGGTCGAAGACCATGACGTTGTTAAATTGTACCACGGCACAAAATTTTAGCACATCCACCACACCTGCTGACACCCCCTTGTGGAAGTACATGGCGATCAAATGTCTCAGTGACCCAGGGCATTATCAAGTTCTGGCTACTACTGAGTATTTTACAATGTCTGACTTGCCAATAGACTCTTGCTCTACTTTGTCAGAACTCTTTCTTCCAGTTCGACATATACAGGGTGATCATATGTATGCTTGTGAACAAGACCCTGGTTTATATCTGAAATGGAATGAGTCGAAATTCCCAGAATGTCAAAACTGTAGTGGAAGGTGCTATTTCAATTACACCACCAACCAAGTCTTGTGTTATCCTCCCATTCCCAGACAAAAACACAAAGGTCTATCAACTAGGTTTATTGTCATCGGAGTGAGCGTATGTGGGCTTGTTGTCTTGTCAACTACAGCGGTAGCCGCCTTTTACCATTTAAAGCAATCAAAGAGGAGAGAGGAAAAGGAGAATCAAAGAAAGGTTGAAAAGTTCTTGGATGATCACAAGTCCCATGTACCAACAAGATACTCCTATGCTGATATAAAGAAGATGACAAGAGGATTCAAGAAGAAGTTAGGTGAAGGAGGTTTTGGAAGTGTTTTCAGTGGAAAGCTTCCCAGTAATGGGGTGCCAGTTGCTGTAAAAGTCCTCAAGGATTCTAAAGGAAGTGGAGAAGACTTTGTTAACGAAGTGGGAACCATTGGCAGAATTCACCATGTTAACGTGGTTCGACTACTTGGGTTTTCAGCTGAAGGAGGGAAGCGTGCTCTTATTTATGAGCTCATGCCAAACAAGTCCCTAGAGAAGTTCATCAAATCTAGAGGATCAAATAATAGCAGCAGTTCATTTAACTGGGAGAAACTTTGCAACATCATCACAGGTATAGCAAAGGGAATCGAGTACCTTCACCAAGGGTGTGACCAGAGGATTCTCCACTTTGATATCAAGCCTCACAATATCTTGTTAGACCATGCCTTTAATCCAAAAATTTCTGATTTTGGTCTGGCTAAACTCTGTTCCAGGGAAGATAGTGTTATATCTATGACTGCTGCCAGAGGCACTATAGGCTACATTGCACCTGAAGTATTTAATGGCAACTTTGGAACTGTCACCTACAAATCTGATGTGTATAGTTTCGGGATGCTGGTGCTTGAAGTTATTGGAGCCAGGAAGGAAACTGCTGTTACATCTAGCGACAATGAGGTGTATTTTCCTGAATTGATTTTCAAGTGTCTGATCCAGGGAGAAGAGTTGGATTTGGAGCTAGCTGATGATGGAGATGCGCAGATTGCTAAGAGATTAGCAATTGTGGCACTTTGGTGCATCCAGTGGTACCCAGTGTATCGCCCTTCCATGAAAGCAGTTGTAAGAATGCTGGAAGGACCCTCTGAAAGCTTGATCATGCCACCCAATCCGTTTGCTTCCACAACTCAGATGGAACCAACAACAACCCTATCAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PCRF RF-1

Protein Analysis

630

Amino Acids

70.69

Weight (kDa)

7.97

Isoelectric Point (pI)

44.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 28 - 96 5e-15 Wall-associated receptor kinase galacturonan-binding
PK_Tyr_Ser-Thr PF07714 331 - 601 7.3e-43 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 332 - 600 1.5e-43 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014362)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G56350
fragaria_vesca FvH4_6g07390
malus_domestica MD04G1193600.v1.1 MD12G1206300.v1.1
prunus_persica Prupe.6G313600_v2.0.a1 Prupe.6G313600_v2.0.a1
pyrus_communis pycom12g19420
rosa_chinensis RchiOBHm_Chr3g0456991
rosa_laevigata RLG00000025255
rosa_multiflora Rmu_co8479901.1_g000001
rosa_roxburghii Rroxscaffold_6G00422290
rosa_rugosa Rorug03G0018100
rosa_samantha Rh3AG077900 Rh3BG079900 Rh3CG079800 Rh3DG081600
rosa_wichuraiana Rw3G006690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 466
Acc16I TGCGCA 1 cut(s) 1713
Acc36I ACCTGC 1 cut(s) 466
Acc65I GGTACC 1 cut(s) 1761
AccB1I GGYRCC 2 cut(s) 1057, 1761
AccI GTMKAC 1 cut(s) 136
AciI CCGC 3 cut(s) 378, 845, 852
AclWI GGATC 2 cut(s) 1261, 1660
AcoI YGGCCR 1 cut(s) 160
AcsI RAATTY 4 cut(s) 436, 680, 1137, 1416
AcuI CTGAAG 2 cut(s) 1200, 1530
AcyI GRCGYC 1 cut(s) 310
AfaI GTAC 5 cut(s) 424, 482, 945, 1331, 1763
AfiI CCNNNNNNNGG 3 cut(s) 474, 1052, 1053
AgsI TTSAA 7 cut(s) 92, 347, 721, 914, 994, 1583, 1657
AjnI CCWGG 5 cut(s) 508, 649, 1448, 1595, 1668
AjuI GAANNNNNNNTTGG 2 cut(s) 1002, 1034
AleI CACNNNNGTG 1 cut(s) 1747
AluBI AGCT 8 cut(s) 192, 217, 1042, 1178, 1210, 1693, 1697, 1824
AluI AGCT 8 cut(s) 192, 217, 1042, 1178, 1210, 1693, 1697, 1824
Alw21I GWGCWC 2 cut(s) 1198, 1212
Alw26I GTCTC 3 cut(s) 301, 334, 503
AlwI GGATC 2 cut(s) 1261, 1660
AlwNI CAGNNNCTG 1 cut(s) 1067
Ama87I CYCGRG 1 cut(s) 304
AoxI GGCC 1 cut(s) 160
ApeKI GCWGC 5 cut(s) 65, 186, 189, 1266, 1478
ApoI RAATTY 4 cut(s) 436, 680, 1137, 1416
Asp700I GAANNNNTTC 1 cut(s) 594
Asp718I GGTACC 1 cut(s) 1761
AspLEI GCGC 1 cut(s) 1714
AspS9I GGNCC 2 cut(s) 110, 1811
AsuHPI GGTGA 6 cut(s) 187, 632, 1019, 1133, 1328, 1531
AsuNHI GCTAGC 1 cut(s) 1693
AvaI CYCGRG 1 cut(s) 304
AvaII GGWCC 2 cut(s) 110, 1811
BalI TGGCCA 1 cut(s) 162
BanI GGYRCC 2 cut(s) 1057, 1761
BanII GRGCYC 1 cut(s) 1212
BbsI GAAGAC 2 cut(s) 407, 1110
Bbv12I GWGCWC 2 cut(s) 1198, 1212
BbvI GCAGC 5 cut(s) 77, 198, 201, 1278, 1465
BccI CCATC 5 cut(s) 101, 171, 287, 1697, 1858
BceAI ACGGC 1 cut(s) 444
BciT130I CCWGG 5 cut(s) 510, 651, 1450, 1597, 1670
BclI TGATCA 5 cut(s) 73, 286, 622, 928, 1827
BcoDI GTCTC 3 cut(s) 301, 334, 503
BfaI CTAG 5 cut(s) 789, 1229, 1248, 1620, 1694
BfmI CTRYAG 4 cut(s) 184, 700, 840, 1491
BfuAI ACCTGC 1 cut(s) 466
BisI GCNGC 6 cut(s) 66, 187, 190, 852, 1267, 1479
BlsI GCNGC 6 cut(s) 67, 188, 191, 853, 1268, 1480
Bme1390I CCNGG 5 cut(s) 510, 651, 1450, 1597, 1670
Bme18I GGWCC 2 cut(s) 110, 1811
BmeT110I CYCGRG 1 cut(s) 304
BmgT120I GGNCC 2 cut(s) 110, 1811
BmiI GGNNCC 7 cut(s) 6, 1059, 1126, 1594, 1763, 1813, 1869
BmrFI CCNGG 5 cut(s) 510, 651, 1450, 1597, 1670
BmrI ACTGGG 5 cut(s) 33, 80, 1041, 1291, 1760
BmsI GCATC 3 cut(s) 1560, 1699, 1761
BmtI GCTAGC 1 cut(s) 1697
BmuI ACTGGG 5 cut(s) 33, 80, 1041, 1291, 1760
BpiI GAAGAC 2 cut(s) 407, 1110
BpuEI CTTGAG 1 cut(s) 1064
Bsa29I ATCGAT 1 cut(s) 255
BsaHI GRCGYC 1 cut(s) 310
BsaJI CCNNGG 7 cut(s) 426, 508, 509, 649, 1339, 1449, 1669
BsaXI ACNNNNNCTCC 2 cut(s) 798, 828
Bsc4I CCNNNNNNNGG 3 cut(s) 474, 1052, 1053
Bse1I ACTGG 8 cut(s) 39, 86, 602, 1047, 1061, 1286, 1756, 1766
Bse3DI GCAATG 1 cut(s) 1500
BseBI CCWGG 5 cut(s) 510, 651, 1450, 1597, 1670
BseCI ATCGAT 1 cut(s) 255
BseDI CCNNGG 7 cut(s) 426, 508, 509, 649, 1339, 1449, 1669
BseGI GGATG 6 cut(s) 163, 298, 446, 931, 1575, 1752
BseLI CCNNNNNNNGG 3 cut(s) 474, 1052, 1053
BseMI GCAATG 1 cut(s) 1500
BseMII CTCAG 3 cut(s) 514, 528, 1874
BseNI ACTGG 8 cut(s) 39, 86, 602, 1047, 1061, 1286, 1756, 1766
BseRI GAGGAG 3 cut(s) 322, 325, 895
BseXI GCAGC 5 cut(s) 77, 198, 201, 1278, 1465
BsgI GTGCAG 1 cut(s) 118
BshFI GGCC 1 cut(s) 162
BshNI GGYRCC 2 cut(s) 1057, 1761
BshVI ATCGAT 1 cut(s) 255
BsiHKAI GWGCWC 2 cut(s) 1198, 1212
BsiHKCI CYCGRG 1 cut(s) 304
BslFI GGGAC 2 cut(s) 922, 1210
BslI CCNNNNNNNGG 3 cut(s) 474, 1052, 1053
BsmAI GTCTC 3 cut(s) 301, 334, 503
BsmBI CGTCTC 2 cut(s) 301, 334
BsmFI GGGAC 2 cut(s) 922, 1210
BsmI GAATGC 1 cut(s) 1806
BsnI GGCC 1 cut(s) 162
BsoBI CYCGRG 1 cut(s) 304
Bsp1286I GDGCHC 2 cut(s) 1198, 1212
Bsp143I GATC 8 cut(s) 73, 286, 489, 622, 928, 1253, 1665, 1827
BspACI CCGC 3 cut(s) 378, 845, 852
BspANI GGCC 1 cut(s) 162
BspCNI CTCAG 3 cut(s) 513, 529, 1873
BspDI ATCGAT 1 cut(s) 255
BspLI GGNNCC 7 cut(s) 6, 1059, 1126, 1594, 1763, 1813, 1869
BspMAI CTGCAG 1 cut(s) 188
BspMI ACCTGC 1 cut(s) 466
BspOI GCTAGC 1 cut(s) 1697
BspPI GGATC 2 cut(s) 1261, 1660
BspT107I GGYRCC 2 cut(s) 1057, 1761
BsrDI GCAATG 1 cut(s) 1500
BsrI ACTGG 8 cut(s) 39, 86, 602, 1047, 1061, 1286, 1756, 1766
BssECI CCNNGG 7 cut(s) 426, 508, 509, 649, 1339, 1449, 1669
BssMI GATC 8 cut(s) 73, 286, 489, 622, 928, 1253, 1665, 1827
BssNI GRCGYC 1 cut(s) 310
BssT1I CCWWGG 1 cut(s) 1339
Bst2UI CCWGG 5 cut(s) 510, 651, 1450, 1597, 1670
Bst4CI ACNGT 5 cut(s) 158, 273, 328, 701, 1537
Bst6I CTCTTC 1 cut(s) 1671
BstACI GRCGYC 1 cut(s) 310
BstC8I GCNNGC 3 cut(s) 101, 1194, 1695
BstDEI CTNAG 4 cut(s) 500, 537, 1722, 1860
BstDSI CCRYGG 1 cut(s) 426
BstF5I GGATG 6 cut(s) 163, 298, 446, 931, 1575, 1752
BstHHI GCGC 1 cut(s) 1714
BstKTI GATC 8 cut(s) 76, 289, 492, 625, 931, 1256, 1668, 1830
BstMAI GTCTC 3 cut(s) 301, 334, 503
BstMBI GATC 8 cut(s) 73, 286, 489, 622, 928, 1253, 1665, 1827
BstMWI GCNNNNNNNGC 5 cut(s) 19, 851, 1494, 1718, 1739
BstNI CCWGG 5 cut(s) 510, 651, 1450, 1597, 1670
BstSCI CCNGG 5 cut(s) 508, 649, 1448, 1595, 1668
BstSFI CTRYAG 4 cut(s) 184, 700, 840, 1491
BstV1I GCAGC 5 cut(s) 77, 198, 201, 1278, 1465
BstV2I GAAGAC 2 cut(s) 407, 1110
Bsu15I ATCGAT 1 cut(s) 255
BsuRI GGCC 1 cut(s) 162
BsuTUI ATCGAT 1 cut(s) 255
BtgI CCRYGG 1 cut(s) 426
BtsCI GGATG 6 cut(s) 163, 298, 446, 931, 1575, 1752
BtsIMutI CAGTG 5 cut(s) 154, 508, 1039, 1763, 1773
BveI ACCTGC 1 cut(s) 466
Cac8I GCNNGC 3 cut(s) 101, 1194, 1695
CaiI CAGNNNCTG 1 cut(s) 1067
CfoI GCGC 1 cut(s) 1714
Cfr13I GGNCC 2 cut(s) 110, 1811
ClaI ATCGAT 1 cut(s) 255
CseI GACGC 1 cut(s) 318
Csp6I GTAC 5 cut(s) 423, 481, 944, 1330, 1762
CviQI GTAC 5 cut(s) 423, 481, 944, 1330, 1762
DdeI CTNAG 4 cut(s) 500, 537, 1722, 1860
DpnI GATC 8 cut(s) 75, 288, 491, 624, 930, 1255, 1667, 1829
DpnII GATC 8 cut(s) 73, 286, 489, 622, 928, 1253, 1665, 1827
DraI TTTAAA 1 cut(s) 867
EaeI YGGCCR 1 cut(s) 160
Eam1104I CTCTTC 1 cut(s) 1671
EarI CTCTTC 1 cut(s) 1671
Ecl136II GAGCTC 1 cut(s) 1210
Eco130I CCWWGG 1 cut(s) 1339
Eco24I GRGCYC 1 cut(s) 1212
Eco32I GATATC 2 cut(s) 118, 1372
Eco47I GGWCC 2 cut(s) 110, 1811
Eco53kI GAGCTC 1 cut(s) 1210
Eco57I CTGAAG 2 cut(s) 1200, 1530
Eco88I CYCGRG 1 cut(s) 304
EcoICRI GAGCTC 1 cut(s) 1210
EcoO109I RGGNCCY 1 cut(s) 1811
EcoRI GAATTC 1 cut(s) 1137
EcoRII CCWGG 5 cut(s) 508, 649, 1448, 1595, 1668
EcoRV GATATC 2 cut(s) 118, 1372
EcoT14I CCWWGG 1 cut(s) 1339
EcoT38I GRGCYC 1 cut(s) 1212
ErhI CCWWGG 1 cut(s) 1339
Esp3I CGTCTC 2 cut(s) 301, 334
FaqI GGGAC 2 cut(s) 922, 1210
FauNDI CATATG 1 cut(s) 627
FbaI TGATCA 5 cut(s) 73, 286, 622, 928, 1827
FblI GTMKAC 1 cut(s) 136
Fnu4HI GCNGC 6 cut(s) 66, 187, 190, 852, 1267, 1479
FokI GGATG 6 cut(s) 150, 305, 433, 938, 1582, 1739
FriOI GRGCYC 1 cut(s) 1212
Fsp4HI GCNGC 6 cut(s) 66, 187, 190, 852, 1267, 1479
FspBI CTAG 5 cut(s) 789, 1229, 1248, 1620, 1694
FspI TGCGCA 1 cut(s) 1713
GlaI GCGC 1 cut(s) 1713
GluI GCNGC 6 cut(s) 66, 187, 190, 852, 1267, 1479
HaeIII GGCC 1 cut(s) 162
HgaI GACGC 1 cut(s) 318
HhaI GCGC 1 cut(s) 1714
Hin1I GRCGYC 1 cut(s) 310
Hin6I GCGC 1 cut(s) 1712
HinP1I GCGC 1 cut(s) 1712
HincII GTYRAC 4 cut(s) 269, 837, 1114, 1150
HindII GTYRAC 4 cut(s) 269, 837, 1114, 1150
HindIII AAGCTT 3 cut(s) 215, 1040, 1822
HpaI GTTAAC 2 cut(s) 1114, 1150
HphI GGTGA 6 cut(s) 187, 632, 1019, 1133, 1328, 1531
Hpy166II GTNNAC 6 cut(s) 137, 269, 641, 837, 1114, 1150
Hpy188I TCNGA 9 cut(s) 556, 589, 663, 806, 1423, 1552, 1665, 1819, 1863
Hpy188III TCNNGA 9 cut(s) 113, 167, 231, 290, 304, 994, 1248, 1567, 1643
Hpy8I GTNNAC 6 cut(s) 137, 269, 641, 837, 1114, 1150
HpyAV CCTTC 9 cut(s) 161, 323, 702, 1004, 1175, 1343, 1594, 1788, 1802
HpyCH4III ACNGT 5 cut(s) 158, 273, 328, 701, 1537
HpyCH4IV ACGT 2 cut(s) 410, 1152
HpyCH4V TGCA 5 cut(s) 99, 186, 1296, 1505, 1752
HpyF10VI GCNNNNNNNGC 5 cut(s) 19, 851, 1494, 1718, 1739
HpyF3I CTNAG 4 cut(s) 500, 537, 1722, 1860
HpySE526I ACGT 2 cut(s) 410, 1152
Hsp92I GRCGYC 1 cut(s) 310
HspAI GCGC 1 cut(s) 1712
KpnI GGTACC 1 cut(s) 1765
Ksp22I TGATCA 5 cut(s) 73, 286, 622, 928, 1827
KspAI GTTAAC 2 cut(s) 1114, 1150
Kzo9I GATC 8 cut(s) 73, 286, 489, 622, 928, 1253, 1665, 1827
LmnI GCTCC 3 cut(s) 4, 1592, 1690
Lsp1109I GCAGC 5 cut(s) 77, 198, 201, 1278, 1465
LweI GCATC 3 cut(s) 1560, 1699, 1761
MaeI CTAG 5 cut(s) 789, 1229, 1248, 1620, 1694
MaeII ACGT 2 cut(s) 410, 1152
MaeIII GTNAC 5 cut(s) 380, 503, 1346, 1537, 1612
MalI GATC 8 cut(s) 75, 288, 491, 624, 930, 1255, 1667, 1829
MboI GATC 8 cut(s) 73, 286, 489, 622, 928, 1253, 1665, 1827
MboII GAAGA 8 cut(s) 377, 412, 590, 988, 1009, 1115, 1466, 1688
MfeI CAATTG 1 cut(s) 1734
MhlI GDGCHC 2 cut(s) 1198, 1212
MlsI TGGCCA 1 cut(s) 162
MluNI TGGCCA 1 cut(s) 162
MlyI GAGTC 3 cut(s) 274, 563, 683
MmeI TCCRAC 1 cut(s) 1662
Mox20I TGGCCA 1 cut(s) 162
MroXI GAANNNNTTC 1 cut(s) 594
MscI TGGCCA 1 cut(s) 162
MslI CAYNNNNRTG 5 cut(s) 200, 206, 630, 806, 1747
Msp20I TGGCCA 1 cut(s) 162
MspA1I CMGCKG 2 cut(s) 845, 1178
MspR9I CCNGG 5 cut(s) 510, 651, 1450, 1597, 1670
MunI CAATTG 1 cut(s) 1734
Mva1269I GAATGC 1 cut(s) 1806
MvaI CCWGG 5 cut(s) 510, 651, 1450, 1597, 1670
MwoI GCNNNNNNNGC 5 cut(s) 19, 851, 1494, 1718, 1739
NdeI CATATG 1 cut(s) 627
NdeII GATC 8 cut(s) 73, 286, 489, 622, 928, 1253, 1665, 1827
NheI GCTAGC 1 cut(s) 1693
NlaIV GGNNCC 7 cut(s) 6, 1059, 1126, 1594, 1763, 1813, 1869
NmuCI GTSAC 3 cut(s) 503, 1346, 1537
NsbI TGCGCA 1 cut(s) 1713
OliI CACNNNNGTG 1 cut(s) 1747
PaqCI CACCTGC 1 cut(s) 466
PasI CCCWGGG 1 cut(s) 509
PcsI WCGNNNNNNNCGW 1 cut(s) 810
PctI GAATGC 1 cut(s) 1806
PdmI GAANNNNTTC 1 cut(s) 594
PfeI GAWTC 7 cut(s) 234, 368, 898, 990, 1085, 1323, 1357
PkrI GCNGC 6 cut(s) 67, 188, 191, 853, 1268, 1480
PleI GAGTC 3 cut(s) 273, 563, 682
PpsI GAGTC 3 cut(s) 273, 563, 682
PpuMI RGGWCCY 1 cut(s) 1811
Psp124BI GAGCTC 1 cut(s) 1212
Psp5II RGGWCCY 1 cut(s) 1811
Psp6I CCWGG 5 cut(s) 508, 649, 1448, 1595, 1668
PspGI CCWGG 5 cut(s) 508, 649, 1448, 1595, 1668
PspN4I GGNNCC 7 cut(s) 6, 1059, 1126, 1594, 1763, 1813, 1869
PspPI GGNCC 2 cut(s) 110, 1811
PspPPI RGGWCCY 1 cut(s) 1811
PstI CTGCAG 1 cut(s) 188
PstNI CAGNNNCTG 1 cut(s) 1067
PvuII CAGCTG 1 cut(s) 1178
RsaI GTAC 5 cut(s) 424, 482, 945, 1331, 1763
RsaNI GTAC 5 cut(s) 423, 481, 944, 1330, 1762
RseI CAYNNNNRTG 5 cut(s) 200, 206, 630, 806, 1747
SacI GAGCTC 1 cut(s) 1212
SatI GCNGC 6 cut(s) 66, 187, 190, 852, 1267, 1479
Sau3AI GATC 8 cut(s) 73, 286, 489, 622, 928, 1253, 1665, 1827
Sau96I GGNCC 2 cut(s) 110, 1811
SchI GAGTC 3 cut(s) 274, 563, 683
ScrFI CCNGG 5 cut(s) 510, 651, 1450, 1597, 1670
SduI GDGCHC 2 cut(s) 1198, 1212
SfaNI GCATC 3 cut(s) 1560, 1699, 1761
SfcI CTRYAG 4 cut(s) 184, 700, 840, 1491
SinI GGWCC 2 cut(s) 110, 1811
SmiMI CAYNNNNRTG 5 cut(s) 200, 206, 630, 806, 1747
SmlI CTYRAG 1 cut(s) 1079
SmoI CTYRAG 1 cut(s) 1079
SsiI CCGC 3 cut(s) 378, 845, 852
SspMI CTAG 5 cut(s) 789, 1229, 1248, 1620, 1694
SstI GAGCTC 1 cut(s) 1212
StyD4I CCNGG 5 cut(s) 508, 649, 1448, 1595, 1668
StyI CCWWGG 1 cut(s) 1339
TaaI ACNGT 5 cut(s) 158, 273, 328, 701, 1537
TaiI ACGT 2 cut(s) 413, 1155
TaqI TCGA 6 cut(s) 255, 398, 607, 677, 1159, 1326
TatI WGTACW 1 cut(s) 480
TauI GCSGC 1 cut(s) 854
TfiI GAWTC 7 cut(s) 234, 368, 898, 990, 1085, 1323, 1357
TscAI CASTG 5 cut(s) 161, 508, 1039, 1763, 1773
TseFI GTSAC 3 cut(s) 503, 1346, 1537
TseI GCWGC 5 cut(s) 65, 186, 189, 1266, 1478
Tsp45I GTSAC 3 cut(s) 503, 1346, 1537
TspDTI ATGAA 4 cut(s) 227, 1228, 1263, 1799
TspGWI ACGGA 3 cut(s) 122, 361, 1833
TspRI CASTG 5 cut(s) 161, 508, 1039, 1763, 1773
VpaK11BI GGWCC 2 cut(s) 110, 1811
XapI RAATTY 4 cut(s) 436, 680, 1137, 1416
XbaI TCTAGA 1 cut(s) 1247
XcmI CCANNNNNNNNNTGG 1 cut(s) 1861
XmiI GTMKAC 1 cut(s) 136
XmnI GAANNNNTTC 1 cut(s) 594
XspI CTAG 5 cut(s) 789, 1229, 1248, 1620, 1694
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.