Rmu_co8479901.1_g000001

Peptide chain release factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8479901.1
OG0014362
Physical Location & Seq
Reverse (-)
1 .. 1979
1979 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8479901.1_g000001.1.cds

Sequence Viewer

Length: 890 bp
atggcagaggatgagagtgattggaagagtcacgctgctgcaattgcccaatcagttcatcttattaagaagcgtttgcagtggaagaagttgctggtgaggttggatatgttatcagtggagctgaataagccagacctttggaatgatcctgtacatgccgggaagataagtcgcgagcatggttcactgatgggaaaaatgaaagaagtgaaggcatttgagcaggaattgcttgagcatgtggacatgataaagcttgctcgggaggagaatgacgcagagttggaatcggaatccatgacggctttgcttgagatgagaagaacttcaaaggagaaagagaccgaagctttgttagctggggagcaggatccttgctcatgttatatagaggtgcaatcgggagctgggggtacagagagcaatgactgggcagcaatggtcatgcagatgtataaaatgtgggctcaacgccgtggatataaaatgaccgtggtggacgaaatgcctggtgagattgcgggaatcaaggttcaccttcttgccatccatcaccaatccttcatcattctattcttgcagcgggcaactatcaaagttgatggtgaatatgcttttggatatgccaaagcagaagtaggagtgcacaggttggtacgtatctcaccttttgacagtaataagcgcagacatacttcatttgctgctgtcgctgtgactccaatcctgggagatgtatctgctcatgtactaattaatgaatctgatctgcgtatcgagcgttttcgttctgggggatctggaggccagcatgctaatacgactgatagtgctgtgaggattgttcacattcccactggaataactgccacttgtcaaaatgaaag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PCRF RF-1

Protein Analysis

296

Amino Acids

33.13

Weight (kDa)

6.13

Isoelectric Point (pI)

48.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014362)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G56350
fragaria_vesca FvH4_6g07390
malus_domestica MD04G1193600.v1.1 MD12G1206300.v1.1
prunus_persica Prupe.6G313600_v2.0.a1 Prupe.6G313600_v2.0.a1
pyrus_communis pycom12g19420
rosa_chinensis RchiOBHm_Chr3g0456991
rosa_laevigata RLG00000025255
rosa_multiflora Rmu_co8479901.1_g000001
rosa_roxburghii Rroxscaffold_6G00422290
rosa_rugosa Rorug03G0018100
rosa_samantha Rh3AG077900 Rh3BG079900 Rh3CG079800 Rh3DG081600
rosa_wichuraiana Rw3G006690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 177
AciI CCGC 2 cut(s) 524, 586
AclWI GGATC 4 cut(s) 143, 368, 381, 808
AfaI GTAC 4 cut(s) 156, 418, 660, 753
AfiI CCNNNNNNNGG 1 cut(s) 731
AgsI TTSAA 1 cut(s) 333
AjnI CCWGG 2 cut(s) 511, 729
AjuI GAANNNNNNNTTGG 2 cut(s) 603, 635
AloI GAACNNNNNNTCC 2 cut(s) 519, 551
AluBI AGCT 5 cut(s) 124, 259, 353, 362, 410
AluI AGCT 5 cut(s) 124, 259, 353, 362, 410
Alw21I GWGCWC 1 cut(s) 651
Alw26I GTCTC 1 cut(s) 338
Alw44I GTGCAC 1 cut(s) 647
AlwI GGATC 4 cut(s) 143, 368, 381, 808
Ama87I CYCGRG 1 cut(s) 264
AoxI GGCC 1 cut(s) 808
ApaLI GTGCAC 1 cut(s) 647
ApeKI GCWGC 5 cut(s) 35, 38, 437, 583, 707
ArsI GACNNNNNNTTYG 2 cut(s) 337, 369
AseI ATTAAT 1 cut(s) 759
Asp700I GAANNNNTTC 1 cut(s) 328
AspLEI GCGC 1 cut(s) 690
AsuC2I CCSGG 1 cut(s) 163
AsuHPI GGTGA 6 cut(s) 109, 527, 530, 548, 620, 660
AvaI CYCGRG 1 cut(s) 264
BaeGI GKGCMC 1 cut(s) 651
BamHI GGATCC 1 cut(s) 373
BanII GRGCYC 1 cut(s) 472
Bbv12I GWGCWC 1 cut(s) 651
BbvI GCAGC 5 cut(s) 22, 25, 449, 595, 694
BccI CCATC 4 cut(s) 187, 557, 561, 599
BceAI ACGGC 2 cut(s) 321, 462
BciT130I CCWGG 2 cut(s) 513, 731
BcnI CCSGG 1 cut(s) 163
BcoDI GTCTC 1 cut(s) 338
BisI GCNGC 5 cut(s) 36, 39, 438, 584, 708
BlsI GCNGC 5 cut(s) 37, 40, 439, 585, 709
Bme1390I CCNGG 3 cut(s) 163, 513, 731
BmeT110I CYCGRG 1 cut(s) 264
BmiI GGNNCC 1 cut(s) 375
BmrFI CCNGG 3 cut(s) 163, 513, 731
BmrI ACTGGG 1 cut(s) 442
BmuI ACTGGG 1 cut(s) 442
BpmI CTGGAG 1 cut(s) 825
BpuEI CTTGAG 2 cut(s) 257, 335
BpuMI CCSGG 1 cut(s) 163
BsaAI YACGTR 1 cut(s) 662
BsaI GGTCTC 1 cut(s) 338
BsaJI CCNNGG 3 cut(s) 478, 495, 730
Bsc4I CCNNNNNNNGG 1 cut(s) 731
Bse1I ACTGG 2 cut(s) 437, 865
Bse3DI GCAATG 2 cut(s) 433, 447
BseBI CCWGG 2 cut(s) 513, 731
BseDI CCNNGG 3 cut(s) 478, 495, 730
BseGI GGATG 2 cut(s) 16, 549
BseLI CCNNNNNNNGG 1 cut(s) 731
BseMI GCAATG 2 cut(s) 433, 447
BseNI ACTGG 2 cut(s) 437, 865
BseRI GAGGAG 1 cut(s) 284
BseSI GKGCMC 1 cut(s) 651
BseXI GCAGC 5 cut(s) 22, 25, 449, 595, 694
BseYI CCCAGC 2 cut(s) 362, 410
Bsh1236I CGCG 1 cut(s) 177
BshFI GGCC 1 cut(s) 810
BsiHKAI GWGCWC 1 cut(s) 651
BsiHKCI CYCGRG 1 cut(s) 264
BsiSI CCGG 1 cut(s) 162
BslI CCNNNNNNNGG 1 cut(s) 731
BsmAI GTCTC 1 cut(s) 338
BsnI GGCC 1 cut(s) 810
Bso31I GGTCTC 1 cut(s) 338
BsoBI CYCGRG 1 cut(s) 264
Bsp1286I GDGCHC 2 cut(s) 472, 651
Bsp1407I TGTACA 1 cut(s) 154
Bsp143I GATC 4 cut(s) 148, 373, 769, 800
Bsp68I TCGCGA 1 cut(s) 177
BspACI CCGC 2 cut(s) 524, 586
BspANI GGCC 1 cut(s) 810
BspFNI CGCG 1 cut(s) 177
BspLI GGNNCC 1 cut(s) 375
BspPI GGATC 4 cut(s) 143, 368, 381, 808
BspTNI GGTCTC 1 cut(s) 338
BsrDI GCAATG 2 cut(s) 433, 447
BsrGI TGTACA 1 cut(s) 154
BsrI ACTGG 2 cut(s) 437, 865
BssECI CCNNGG 3 cut(s) 478, 495, 730
BssMI GATC 4 cut(s) 148, 373, 769, 800
Bst2UI CCWGG 2 cut(s) 513, 731
Bst4CI ACNGT 2 cut(s) 496, 680
Bst6I CTCTTC 1 cut(s) 20
BstAPI GCANNNNNTGC 1 cut(s) 232
BstAUI TGTACA 1 cut(s) 154
BstBAI YACGTR 1 cut(s) 662
BstC8I GCNNGC 5 cut(s) 179, 261, 588, 812, 816
BstDSI CCRYGG 2 cut(s) 478, 495
BstF5I GGATG 2 cut(s) 16, 549
BstFNI CGCG 1 cut(s) 177
BstHHI GCGC 1 cut(s) 690
BstKTI GATC 4 cut(s) 151, 376, 772, 803
BstMAI GTCTC 1 cut(s) 338
BstMBI GATC 4 cut(s) 148, 373, 769, 800
BstMWI GCNNNNNNNGC 6 cut(s) 44, 130, 232, 359, 713, 781
BstNI CCWGG 2 cut(s) 513, 731
BstNSI RCATGY 3 cut(s) 161, 245, 818
BstSCI CCNGG 3 cut(s) 161, 511, 729
BstSLI GKGCMC 1 cut(s) 651
BstSNI TACGTA 1 cut(s) 662
BstUI CGCG 1 cut(s) 177
BstV1I GCAGC 5 cut(s) 22, 25, 449, 595, 694
BstX2I RGATCY 2 cut(s) 373, 800
BstXI CCANNNNNNTGG 1 cut(s) 141
BstYI RGATCY 2 cut(s) 373, 800
BsuRI GGCC 1 cut(s) 810
BtgI CCRYGG 2 cut(s) 478, 495
BtsCI GGATG 2 cut(s) 16, 549
BtsI GCAGTG 1 cut(s) 86
BtsIMutI CAGTG 4 cut(s) 86, 123, 188, 858
BtuMI TCGCGA 1 cut(s) 177
Cac8I GCNNGC 5 cut(s) 179, 261, 588, 812, 816
CfoI GCGC 1 cut(s) 690
CseI GACGC 1 cut(s) 287
Csp6I GTAC 4 cut(s) 155, 417, 659, 752
CviAII CATG 9 cut(s) 158, 182, 242, 250, 301, 384, 448, 749, 815
CviJI RGCY 9 cut(s) 124, 133, 259, 308, 353, 362, 410, 470, 810
CviKI_1 RGCY 9 cut(s) 124, 133, 259, 308, 353, 362, 410, 470, 810
CviQI GTAC 4 cut(s) 155, 417, 659, 752
DpnI GATC 4 cut(s) 150, 375, 771, 802
DpnII GATC 4 cut(s) 148, 373, 769, 800
Eam1104I CTCTTC 1 cut(s) 20
EarI CTCTTC 1 cut(s) 20
Eco105I TACGTA 1 cut(s) 662
Eco24I GRGCYC 1 cut(s) 472
Eco31I GGTCTC 1 cut(s) 338
Eco88I CYCGRG 1 cut(s) 264
EcoRII CCWGG 2 cut(s) 511, 729
EcoT38I GRGCYC 1 cut(s) 472
FaeI CATG 9 cut(s) 161, 185, 245, 253, 304, 387, 451, 752, 818
FatI CATG 9 cut(s) 157, 181, 241, 249, 300, 383, 447, 748, 814
FauI CCCGC 2 cut(s) 517, 579
Fnu4HI GCNGC 5 cut(s) 36, 39, 438, 584, 708
FokI GGATG 2 cut(s) 23, 536
FriOI GRGCYC 1 cut(s) 472
Fsp4HI GCNGC 5 cut(s) 36, 39, 438, 584, 708
GlaI GCGC 1 cut(s) 689
GluI GCNGC 5 cut(s) 36, 39, 438, 584, 708
GsaI CCCAGC 2 cut(s) 366, 414
GsuI CTGGAG 1 cut(s) 825
HaeIII GGCC 1 cut(s) 810
HapII CCGG 1 cut(s) 162
HgaI GACGC 1 cut(s) 287
HhaI GCGC 1 cut(s) 690
Hin1II CATG 9 cut(s) 161, 185, 245, 253, 304, 387, 451, 752, 818
Hin6I GCGC 1 cut(s) 688
HinP1I GCGC 1 cut(s) 688
HindIII AAGCTT 2 cut(s) 257, 351
HinfI GANTC 6 cut(s) 28, 290, 296, 528, 721, 764
HpaII CCGG 1 cut(s) 162
HphI GGTGA 6 cut(s) 109, 527, 530, 548, 620, 660
Hpy166II GTNNAC 6 cut(s) 188, 247, 502, 538, 649, 850
Hpy188I TCNGA 2 cut(s) 295, 769
Hpy188III TCNNGA 4 cut(s) 176, 266, 405, 804
Hpy8I GTNNAC 6 cut(s) 188, 247, 502, 538, 649, 850
HpyAV CCTTC 3 cut(s) 208, 551, 574
HpyCH4III ACNGT 2 cut(s) 496, 680
HpyCH4IV ACGT 1 cut(s) 661
HpyCH4V TGCA 6 cut(s) 41, 79, 400, 451, 583, 649
HpyF10VI GCNNNNNNNGC 6 cut(s) 44, 130, 232, 359, 713, 781
HpySE526I ACGT 1 cut(s) 661
Hsp92II CATG 9 cut(s) 161, 185, 245, 253, 304, 387, 451, 752, 818
HspAI GCGC 1 cut(s) 688
Kzo9I GATC 4 cut(s) 148, 373, 769, 800
LmnI GCTCC 3 cut(s) 121, 367, 407
Lsp1109I GCAGC 5 cut(s) 22, 25, 449, 595, 694
MaeII ACGT 1 cut(s) 661
MaeIII GTNAC 2 cut(s) 29, 718
MalI GATC 4 cut(s) 150, 375, 771, 802
MboI GATC 4 cut(s) 148, 373, 769, 800
MboII GAAGA 4 cut(s) 37, 97, 178, 336
MfeI CAATTG 1 cut(s) 42
MflI RGATCY 2 cut(s) 373, 800
MhlI GDGCHC 2 cut(s) 472, 651
MluCI AATT 3 cut(s) 42, 230, 756
MlyI GAGTC 2 cut(s) 37, 715
MmeI TCCRAC 2 cut(s) 84, 267
MnlI CCTC 5 cut(s) 93, 262, 388, 800, 834
MroXI GAANNNNTTC 1 cut(s) 328
MseI TTAA 2 cut(s) 66, 759
MslI CAYNNNNRTG 1 cut(s) 452
MspA1I CMGCKG 1 cut(s) 586
MspI CCGG 1 cut(s) 162
MspR9I CCNGG 3 cut(s) 163, 513, 731
MunI CAATTG 1 cut(s) 42
MvaI CCWGG 2 cut(s) 513, 731
MvnI CGCG 1 cut(s) 177
MwoI GCNNNNNNNGC 6 cut(s) 44, 130, 232, 359, 713, 781
NciI CCSGG 1 cut(s) 163
NdeII GATC 4 cut(s) 148, 373, 769, 800
NlaIII CATG 9 cut(s) 161, 185, 245, 253, 304, 387, 451, 752, 818
NlaIV GGNNCC 1 cut(s) 375
NmuCI GTSAC 2 cut(s) 29, 718
NruI TCGCGA 1 cut(s) 177
NspI RCATGY 3 cut(s) 161, 245, 818
PaeI GCATGC 1 cut(s) 818
PdmI GAANNNNTTC 1 cut(s) 328
PfeI GAWTC 4 cut(s) 290, 296, 528, 764
PkrI GCNGC 5 cut(s) 37, 40, 439, 585, 709
PleI GAGTC 2 cut(s) 36, 715
PpsI GAGTC 2 cut(s) 36, 715
Ppu21I YACGTR 1 cut(s) 662
PshBI ATTAAT 1 cut(s) 759
Psp6I CCWGG 2 cut(s) 511, 729
PspFI CCCAGC 2 cut(s) 362, 410
PspGI CCWGG 2 cut(s) 511, 729
PspN4I GGNNCC 1 cut(s) 375
PsuI RGATCY 2 cut(s) 373, 800
RruI TCGCGA 1 cut(s) 177
RsaI GTAC 4 cut(s) 156, 418, 660, 753
RsaNI GTAC 4 cut(s) 155, 417, 659, 752
RseI CAYNNNNRTG 1 cut(s) 452
SaqAI TTAA 2 cut(s) 66, 759
SatI GCNGC 5 cut(s) 36, 39, 438, 584, 708
Sau3AI GATC 4 cut(s) 148, 373, 769, 800
SchI GAGTC 2 cut(s) 37, 715
ScrFI CCNGG 3 cut(s) 163, 513, 731
SduI GDGCHC 2 cut(s) 472, 651
SmiMI CAYNNNNRTG 1 cut(s) 452
SmlI CTYRAG 2 cut(s) 236, 314
SmoI CTYRAG 2 cut(s) 236, 314
SnaBI TACGTA 1 cut(s) 662
SphI GCATGC 1 cut(s) 818
Sse9I AATT 3 cut(s) 42, 230, 756
SsiI CCGC 2 cut(s) 524, 586
StyD4I CCNGG 3 cut(s) 161, 511, 729
TaaI ACNGT 2 cut(s) 496, 680
TaiI ACGT 1 cut(s) 664
TaqI TCGA 1 cut(s) 780
TaqII GACCGA 1 cut(s) 362
TasI AATT 3 cut(s) 42, 230, 756
TatI WGTACW 2 cut(s) 154, 751
TfiI GAWTC 4 cut(s) 290, 296, 528, 764
Tru1I TTAA 2 cut(s) 66, 759
Tru9I TTAA 2 cut(s) 66, 759
TscAI CASTG 4 cut(s) 86, 123, 195, 865
TseFI GTSAC 2 cut(s) 29, 718
TseI GCWGC 5 cut(s) 35, 38, 437, 583, 707
Tsp45I GTSAC 2 cut(s) 29, 718
TspDTI ATGAA 5 cut(s) 47, 218, 556, 690, 777
TspRI CASTG 4 cut(s) 86, 123, 195, 865
VneI GTGCAC 1 cut(s) 647
VspI ATTAAT 1 cut(s) 759
XceI RCATGY 3 cut(s) 161, 245, 818
XmnI GAANNNNTTC 1 cut(s) 328
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.