MD04G1193600.v1.1

Peptide chain release factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0014362
Physical Location & Seq
Reverse (-)
28340828 .. 28342200
1373 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1193600.v1.1.491

Sequence Viewer

Length: 561 bp
ATGGAATCCATGAACGCTTTGCTTAATATGAGAAGAACTTCAAAGGAGAATGAGATTGAAGCTTTGTTAGCTGGGGAGCAGGATCATTGCATCGTTCAAGCAGGAGATGGGGGTACAGAGAGTAATGACTGGGCAGCTATGGTAATGCAGATTTATAAAATGTGGGCTCAACGATGCAGATATGACATGACTGTGGTGGATGAAATGCCGGGTGAGATTGTGGGAATCAAGATCAATCTGTTCTTGCCGTGGGCAACAATCAAAGTTGACGGTGAATATGCTTTTGGATATGCCAAAGCAGAAGTAGGAGTGCACAGGTTGGTATGGATCTCACCTTTTGACAGTAATAAGCGTAGGCACACTTCATTTGCTGCTGTTGCTGTGACTCCAATCCTGGGAGATGCATCTGAGCATGTACAAATTAATGAATCTGATCTGCGCATTGAGCGATTTCGTTCTGGGGGAGCTGGAGGCCAGCACGCAAATACAACCGATAGTGCTGTGAGGATTGTTCACATTCCTACAGGAATTACTGCTACTTGTCAAAATGAGAGGTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PCRF RF-1

Protein Analysis

187

Amino Acids

20.63

Weight (kDa)

5.5

Isoelectric Point (pI)

42.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCRF PF03462 13 - 126 2.9e-28 PCRF domain
RF-1 PF00472 138 - 185 6.5e-19 RF-1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014362)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G56350
fragaria_vesca FvH4_6g07390
malus_domestica MD04G1193600.v1.1 MD12G1206300.v1.1
prunus_persica Prupe.6G313600_v2.0.a1 Prupe.6G313600_v2.0.a1
pyrus_communis pycom12g19420
rosa_chinensis RchiOBHm_Chr3g0456991
rosa_laevigata RLG00000025255
rosa_multiflora Rmu_co8479901.1_g000001
rosa_roxburghii Rroxscaffold_6G00422290
rosa_rugosa Rorug03G0018100
rosa_samantha Rh3AG077900 Rh3BG079900 Rh3CG079800 Rh3DG081600
rosa_wichuraiana Rw3G006690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 156
Acc16I TGCGCA 1 cut(s) 440
AclWI GGATC 2 cut(s) 90, 335
AfaI GTAC 2 cut(s) 115, 417
AfiI CCNNNNNNNGG 1 cut(s) 395
AgsI TTSAA 3 cut(s) 42, 59, 98
AjnI CCWGG 1 cut(s) 393
AjuI GAANNNNNNNTTGG 2 cut(s) 267, 299
AluBI AGCT 4 cut(s) 62, 71, 137, 467
AluI AGCT 4 cut(s) 62, 71, 137, 467
Alw21I GWGCWC 1 cut(s) 315
Alw44I GTGCAC 1 cut(s) 311
AlwI GGATC 2 cut(s) 90, 335
AoxI GGCC 1 cut(s) 472
ApaLI GTGCAC 1 cut(s) 311
ApeKI GCWGC 2 cut(s) 134, 371
AseI ATTAAT 1 cut(s) 423
Asp700I GAANNNNTTC 1 cut(s) 37
AspLEI GCGC 1 cut(s) 441
AsuC2I CCSGG 1 cut(s) 210
AsuHPI GGTGA 3 cut(s) 224, 284, 324
BaeGI GKGCMC 1 cut(s) 315
BanII GRGCYC 1 cut(s) 169
BarI GAAGNNNNNNTAC 2 cut(s) 346, 378
Bbv12I GWGCWC 1 cut(s) 315
BbvI GCAGC 2 cut(s) 146, 358
BccI CCATC 1 cut(s) 101
BceAI ACGGC 1 cut(s) 232
BciT130I CCWGG 1 cut(s) 395
BcnI CCSGG 1 cut(s) 210
BfmI CTRYAG 1 cut(s) 522
BisI GCNGC 2 cut(s) 135, 372
BlsI GCNGC 2 cut(s) 136, 373
Bme1390I CCNGG 2 cut(s) 210, 395
BmrFI CCNGG 2 cut(s) 210, 395
BmrI ACTGGG 1 cut(s) 139
BmsI GCATC 4 cut(s) 99, 164, 391, 413
BmuI ACTGGG 1 cut(s) 139
BpmI CTGGAG 1 cut(s) 489
BpuMI CCSGG 1 cut(s) 210
BsaJI CCNNGG 2 cut(s) 248, 394
Bsc4I CCNNNNNNNGG 1 cut(s) 395
Bse1I ACTGG 1 cut(s) 134
Bse3DI GCAATG 1 cut(s) 85
BseBI CCWGG 1 cut(s) 395
BseDI CCNNGG 2 cut(s) 248, 394
BseGI GGATG 1 cut(s) 205
BseLI CCNNNNNNNGG 1 cut(s) 395
BseMI GCAATG 1 cut(s) 85
BseMII CTCAG 1 cut(s) 399
BseNI ACTGG 1 cut(s) 134
BseSI GKGCMC 1 cut(s) 315
BseXI GCAGC 2 cut(s) 146, 358
BseYI CCCAGC 1 cut(s) 71
BshFI GGCC 1 cut(s) 474
BsiHKAI GWGCWC 1 cut(s) 315
BsiSI CCGG 1 cut(s) 209
BslI CCNNNNNNNGG 1 cut(s) 395
BsnI GGCC 1 cut(s) 474
Bsp1286I GDGCHC 2 cut(s) 169, 315
Bsp1407I TGTACA 1 cut(s) 415
Bsp143I GATC 4 cut(s) 82, 231, 327, 433
BspANI GGCC 1 cut(s) 474
BspCNI CTCAG 1 cut(s) 400
BspPI GGATC 2 cut(s) 90, 335
BsrDI GCAATG 1 cut(s) 85
BsrGI TGTACA 1 cut(s) 415
BsrI ACTGG 1 cut(s) 134
BssECI CCNNGG 2 cut(s) 248, 394
BssMI GATC 4 cut(s) 82, 231, 327, 433
Bst2UI CCWGG 1 cut(s) 395
Bst4CI ACNGT 3 cut(s) 193, 272, 344
BstAUI TGTACA 1 cut(s) 415
BstC8I GCNNGC 2 cut(s) 476, 480
BstDEI CTNAG 1 cut(s) 408
BstDSI CCRYGG 1 cut(s) 248
BstF5I GGATG 1 cut(s) 205
BstHHI GCGC 1 cut(s) 441
BstKTI GATC 4 cut(s) 85, 234, 330, 436
BstMBI GATC 4 cut(s) 82, 231, 327, 433
BstMWI GCNNNNNNNGC 3 cut(s) 68, 377, 445
BstNI CCWGG 1 cut(s) 395
BstNSI RCATGY 1 cut(s) 416
BstSCI CCNGG 2 cut(s) 208, 393
BstSFI CTRYAG 1 cut(s) 522
BstSLI GKGCMC 1 cut(s) 315
BstV1I GCAGC 2 cut(s) 146, 358
BstX2I RGATCY 1 cut(s) 327
BstYI RGATCY 1 cut(s) 327
BsuRI GGCC 1 cut(s) 474
BtgI CCRYGG 1 cut(s) 248
BtsCI GGATG 1 cut(s) 205
Cac8I GCNNGC 2 cut(s) 476, 480
CfoI GCGC 1 cut(s) 441
Csp6I GTAC 2 cut(s) 114, 416
CviAII CATG 3 cut(s) 10, 187, 413
CviJI RGCY 6 cut(s) 62, 71, 137, 167, 467, 474
CviKI_1 RGCY 6 cut(s) 62, 71, 137, 167, 467, 474
CviQI GTAC 2 cut(s) 114, 416
DdeI CTNAG 1 cut(s) 408
DpnI GATC 4 cut(s) 84, 233, 329, 435
DpnII GATC 4 cut(s) 82, 231, 327, 433
Eco24I GRGCYC 1 cut(s) 169
EcoRII CCWGG 1 cut(s) 393
EcoT22I ATGCAT 1 cut(s) 406
EcoT38I GRGCYC 1 cut(s) 169
FaeI CATG 3 cut(s) 13, 190, 416
FatI CATG 3 cut(s) 9, 186, 412
Fnu4HI GCNGC 2 cut(s) 135, 372
FokI GGATG 1 cut(s) 212
FriOI GRGCYC 1 cut(s) 169
Fsp4HI GCNGC 2 cut(s) 135, 372
FspI TGCGCA 1 cut(s) 440
GlaI GCGC 1 cut(s) 440
GluI GCNGC 2 cut(s) 135, 372
GsaI CCCAGC 1 cut(s) 75
GsuI CTGGAG 1 cut(s) 489
HaeIII GGCC 1 cut(s) 474
HapII CCGG 1 cut(s) 209
HhaI GCGC 1 cut(s) 441
Hin1II CATG 3 cut(s) 13, 190, 416
Hin6I GCGC 1 cut(s) 439
HinP1I GCGC 1 cut(s) 439
HincII GTYRAC 1 cut(s) 268
HindII GTYRAC 1 cut(s) 268
HindIII AAGCTT 1 cut(s) 60
HinfI GANTC 4 cut(s) 5, 225, 385, 428
HpaII CCGG 1 cut(s) 209
HphI GGTGA 3 cut(s) 224, 284, 324
Hpy166II GTNNAC 3 cut(s) 268, 313, 514
Hpy188I TCNGA 2 cut(s) 409, 433
Hpy188III TCNNGA 1 cut(s) 229
Hpy8I GTNNAC 3 cut(s) 268, 313, 514
HpyCH4III ACNGT 3 cut(s) 193, 272, 344
HpyCH4V TGCA 5 cut(s) 90, 148, 177, 313, 404
HpyF10VI GCNNNNNNNGC 3 cut(s) 68, 377, 445
HpyF3I CTNAG 1 cut(s) 408
Hsp92II CATG 3 cut(s) 13, 190, 416
HspAI GCGC 1 cut(s) 439
Kzo9I GATC 4 cut(s) 82, 231, 327, 433
LmnI GCTCC 2 cut(s) 76, 464
Lsp1109I GCAGC 2 cut(s) 146, 358
LweI GCATC 4 cut(s) 99, 164, 391, 413
MaeIII GTNAC 1 cut(s) 382
MalI GATC 4 cut(s) 84, 233, 329, 435
MboI GATC 4 cut(s) 82, 231, 327, 433
MboII GAAGA 1 cut(s) 45
MflI RGATCY 1 cut(s) 327
MhlI GDGCHC 2 cut(s) 169, 315
MluCI AATT 2 cut(s) 420, 528
MlyI GAGTC 1 cut(s) 379
MnlI CCTC 3 cut(s) 464, 498, 546
Mph1103I ATGCAT 1 cut(s) 406
MroXI GAANNNNTTC 1 cut(s) 37
MseI TTAA 3 cut(s) 24, 423, 559
MslI CAYNNNNRTG 1 cut(s) 191
MspI CCGG 1 cut(s) 209
MspR9I CCNGG 2 cut(s) 210, 395
MvaI CCWGG 1 cut(s) 395
MwoI GCNNNNNNNGC 3 cut(s) 68, 377, 445
NciI CCSGG 1 cut(s) 210
NdeII GATC 4 cut(s) 82, 231, 327, 433
NlaIII CATG 3 cut(s) 13, 190, 416
NmuCI GTSAC 1 cut(s) 382
NsbI TGCGCA 1 cut(s) 440
NsiI ATGCAT 1 cut(s) 406
NspI RCATGY 1 cut(s) 416
PdmI GAANNNNTTC 1 cut(s) 37
PfeI GAWTC 3 cut(s) 5, 225, 428
PkrI GCNGC 2 cut(s) 136, 373
PleI GAGTC 1 cut(s) 379
PpsI GAGTC 1 cut(s) 379
PshBI ATTAAT 1 cut(s) 423
PsiI TTATAA 1 cut(s) 156
Psp6I CCWGG 1 cut(s) 393
PspFI CCCAGC 1 cut(s) 71
PspGI CCWGG 1 cut(s) 393
PsuI RGATCY 1 cut(s) 327
RsaI GTAC 2 cut(s) 115, 417
RsaNI GTAC 2 cut(s) 114, 416
RseI CAYNNNNRTG 1 cut(s) 191
SaqAI TTAA 3 cut(s) 24, 423, 559
SatI GCNGC 2 cut(s) 135, 372
Sau3AI GATC 4 cut(s) 82, 231, 327, 433
SchI GAGTC 1 cut(s) 379
ScrFI CCNGG 2 cut(s) 210, 395
SduI GDGCHC 2 cut(s) 169, 315
SetI ASST 7 cut(s) 64, 73, 139, 320, 337, 469, 557
SfaNI GCATC 4 cut(s) 99, 164, 391, 413
SfcI CTRYAG 1 cut(s) 522
SmiMI CAYNNNNRTG 1 cut(s) 191
Sse9I AATT 2 cut(s) 420, 528
StyD4I CCNGG 2 cut(s) 208, 393
TaaI ACNGT 3 cut(s) 193, 272, 344
TasI AATT 2 cut(s) 420, 528
TatI WGTACW 1 cut(s) 415
TfiI GAWTC 3 cut(s) 5, 225, 428
Tru1I TTAA 3 cut(s) 24, 423, 559
Tru9I TTAA 3 cut(s) 24, 423, 559
TseFI GTSAC 1 cut(s) 382
TseI GCWGC 2 cut(s) 134, 371
Tsp45I GTSAC 1 cut(s) 382
TspDTI ATGAA 4 cut(s) 26, 216, 354, 441
VneI GTGCAC 1 cut(s) 311
VspI ATTAAT 1 cut(s) 423
XceI RCATGY 1 cut(s) 416
XmnI GAANNNNTTC 1 cut(s) 37
Zsp2I ATGCAT 1 cut(s) 406
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.