RLG00000025255

Peptide chain release factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0014362
Physical Location & Seq
Reverse (-)
43414759 .. 43417564
2806 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025255

Sequence Viewer

Length: 1545 bp
ATGTCATCTTTCCTGTTCAGAAAAAGAGCAAGTAGTAGTAGATGCTGGGGGGTTCTCTGCCGAAACCCTAAAGCTGAATCTCAATTTGGTAATCTCAATAAACCCCCGAAACCTCAAAATGTCACAAGCGCAGTTGAAATTCCCAGGATTTTGGATCCTGGGTCTTCTTCATTTTCACCCAAAACCTCAAAAACCCTATCTGGGTCTCTCCAGAACATCAAAAACTCAATTGCTTCCGGTGAAAGTTTTGGGCTTTCTTCTGATTTGGGGGTTCCAGTTGTGAGAAGCTTATGTGGGTTTCATGGTGGTATTGCAACTTGTAGCTTGGTTCGATTTTATGGTACTCAGGCTGCTCAGGAGCCTTCGACCTCTGATGGGCTCACAGTTGATCGAATCCTTAATAGTGAGTGGTCGATTCTGGCAGAGGATGAGAGTGATTGGAAGAGTCACGCTGCTGCAATTGCCCAATCAGTTCATCTTATTAAGAAGCGTTTGCAGTGGAAGAAGTTGCTGGTGAGGTTGGATATGTTATCAGTGGAGCTGAATAAGCCAGACCTTTGGAATGATCCTGTACATGCAGGAAAGATAAGCCGCGAGCATGGTTCATTGATGGGGAAAATGAAAGAAGTGAAGGCATTTGAGCAGGAATTGGTTGAGCATGTGGACATGATAAAGCTTGCTCGGGAGGAGAATGACGCGGAGTTGGAATCGGAATCCATGAAGGCTTTGCTTGAGATGAGAAGAACTTCAAAGGAGAAAGAGACCCAAGCTTTGTTGGCTGGGGAGCAGGATCCTTGCTCATGTTATATAGAGGTGCAATCGGGAGCTGGGGGTACAGAGAGCAATGACTGGGCAGCGATGGTCATGCAGATGTATAAAATGTGGGCTCAACGTCGTGGATATAAAATGACCATGGTGGATGAAATGCCTGGTGAGATTGCGGGAATCAAGCGGGCAACTATCAAAGTTGATGGTGAGTATGCTTTTGGATATGCCAAAGCAGAAGTAGGAGTGCACAGGTTGGTACGTATCTCACCTTTTGACAGTAATAAGCGCAGACATACTTCATTTGCTGCTGTCGCTGTGACTCCAATCCTGGGAGATGTATCTGCTCATGTACAAATTAATGAATCTGATCTGCGTATCGAGCGTTTTCGTTCGGGGGGATCTGGAGGCCAGCACGCTAATACGACTGATAGTGCTGTGAGGATTGTTCACATTCCCACTGGAATAACTGCCACTTGTCAAAATGAGAGATCACAACATCAGAATAAGGCTTCAGCAATGGCTGTTATCCAAGCAAGAGTGGACCAGCTTGAAATGGCACGCCAGACTCAGAAGAATGCACAATATTCACAATCTCTTACTGATATAACCTGGGGCAGCCAGATACGCAGCTACGTGCTTCAGCCATACCGCATGGTGAAAGATCTCAGAACCAATTATGAAGTCTCTGATCCTGATTCTGTCCTTGAGGGAGATCTAGACGGTTTTATCTTGAGTTATTTATCAGCTTCCTTGGATAAAGATGAAGATGACCGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PCRF RF-1

Protein Analysis

515

Amino Acids

56.97

Weight (kDa)

6.66

Isoelectric Point (pI)

47.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCRF PF03462 173 - 360 4.3e-51 PCRF domain
RF-1 PF00472 372 - 479 6.5e-36 RF-1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014362)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G56350
fragaria_vesca FvH4_6g07390
malus_domestica MD04G1193600.v1.1 MD12G1206300.v1.1
prunus_persica Prupe.6G313600_v2.0.a1 Prupe.6G313600_v2.0.a1
pyrus_communis pycom12g19420
rosa_chinensis RchiOBHm_Chr3g0456991
rosa_laevigata RLG00000025255
rosa_multiflora Rmu_co8479901.1_g000001
rosa_roxburghii Rroxscaffold_6G00422290
rosa_rugosa Rorug03G0018100
rosa_samantha Rh3AG077900 Rh3BG079900 Rh3CG079800 Rh3DG081600
rosa_wichuraiana Rw3G006690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 594, 698
AciI CCGC 6 cut(s) 592, 698, 941, 952, 1417, 1540
AclWI GGATC 7 cut(s) 149, 162, 560, 785, 798, 1174, 1451
AcsI RAATTY 1 cut(s) 138
AcuI CTGAAG 2 cut(s) 1263, 1391
AfaI GTAC 5 cut(s) 343, 573, 835, 1026, 1119
AfiI CCNNNNNNNGG 3 cut(s) 201, 375, 1097
AgsI TTSAA 3 cut(s) 137, 750, 1319
AjnI CCWGG 5 cut(s) 143, 157, 928, 1095, 1376
AjuI GAANNNNNNNTTGG 2 cut(s) 69, 101
Alw21I GWGCWC 1 cut(s) 1017
Alw26I GTCTC 3 cut(s) 210, 755, 1456
Alw44I GTGCAC 1 cut(s) 1013
AlwI GGATC 7 cut(s) 149, 162, 560, 785, 798, 1174, 1451
Ama87I CYCGRG 1 cut(s) 681
AoxI GGCC 1 cut(s) 1174
ApaLI GTGCAC 1 cut(s) 1013
ApeKI GCWGC 7 cut(s) 350, 452, 455, 854, 1073, 1383, 1395
ApoI RAATTY 1 cut(s) 138
ArsI GACNNNNNNTTYG 2 cut(s) 754, 786
AseI ATTAAT 1 cut(s) 1125
Asp700I GAANNNNTTC 1 cut(s) 745
AspLEI GCGC 2 cut(s) 131, 1056
AspS9I GGNCC 1 cut(s) 1309
AsuHPI GGTGA 7 cut(s) 168, 251, 526, 944, 986, 1026, 1435
AvaI CYCGRG 1 cut(s) 681
AvaII GGWCC 1 cut(s) 1309
BaeGI GKGCMC 1 cut(s) 1017
BamHI GGATCC 2 cut(s) 154, 790
BanII GRGCYC 2 cut(s) 381, 889
BbsI GAAGAC 1 cut(s) 156
Bbv12I GWGCWC 1 cut(s) 1017
BbvI GCAGC 7 cut(s) 337, 439, 442, 866, 1060, 1395, 1407
BccI CCATC 4 cut(s) 368, 604, 853, 965
BcgI CGANNNNNNTGC 2 cut(s) 847, 881
BciT130I CCWGG 5 cut(s) 145, 159, 930, 1097, 1378
BcoDI GTCTC 3 cut(s) 210, 755, 1456
BfaI CTAG 1 cut(s) 1484
BglII AGATCT 2 cut(s) 1429, 1480
BisI GCNGC 8 cut(s) 351, 453, 456, 592, 855, 1074, 1384, 1396
BlsI GCNGC 8 cut(s) 352, 454, 457, 593, 856, 1075, 1385, 1397
Bme1390I CCNGG 5 cut(s) 145, 159, 930, 1097, 1378
Bme18I GGWCC 1 cut(s) 1309
BmeT110I CYCGRG 1 cut(s) 681
BmgT120I GGNCC 1 cut(s) 1309
BmiI GGNNCC 4 cut(s) 156, 273, 360, 792
BmrFI CCNGG 5 cut(s) 145, 159, 930, 1097, 1378
BmrI ACTGGG 1 cut(s) 859
BmsI GCATC 1 cut(s) 32
BmuI ACTGGG 1 cut(s) 859
BpiI GAAGAC 1 cut(s) 156
BpmI CTGGAG 2 cut(s) 194, 1191
Bpu10I CCTNAGC 1 cut(s) 354
BpuEI CTTGAG 3 cut(s) 752, 1493, 1519
BsaAI YACGTR 2 cut(s) 1028, 1402
BsaI GGTCTC 2 cut(s) 210, 755
BsaJI CCNNGG 6 cut(s) 143, 158, 912, 1096, 1377, 1518
BsaWI WCCGGW 1 cut(s) 236
Bsc4I CCNNNNNNNGG 3 cut(s) 201, 375, 1097
Bse1I ACTGG 3 cut(s) 275, 854, 1231
Bse3DI GCAATG 2 cut(s) 850, 1290
BseBI CCWGG 5 cut(s) 145, 159, 930, 1097, 1378
BseDI CCNNGG 6 cut(s) 143, 158, 912, 1096, 1377, 1518
BseGI GGATG 2 cut(s) 433, 925
BseLI CCNNNNNNNGG 3 cut(s) 201, 375, 1097
BseMI GCAATG 2 cut(s) 850, 1290
BseMII CTCAG 4 cut(s) 359, 368, 1349, 1447
BseNI ACTGG 3 cut(s) 275, 854, 1231
BseRI GAGGAG 1 cut(s) 701
BseSI GKGCMC 1 cut(s) 1017
BseXI GCAGC 7 cut(s) 337, 439, 442, 866, 1060, 1395, 1407
BseYI CCCAGC 3 cut(s) 45, 779, 827
Bsh1236I CGCG 2 cut(s) 594, 698
BshFI GGCC 1 cut(s) 1176
BsiHKAI GWGCWC 1 cut(s) 1017
BsiHKCI CYCGRG 1 cut(s) 681
BsiSI CCGG 1 cut(s) 237
BslI CCNNNNNNNGG 3 cut(s) 201, 375, 1097
BsmAI GTCTC 3 cut(s) 210, 755, 1456
BsmI GAATGC 1 cut(s) 1348
BsnI GGCC 1 cut(s) 1176
Bso31I GGTCTC 2 cut(s) 210, 755
BsoBI CYCGRG 1 cut(s) 681
Bsp1286I GDGCHC 3 cut(s) 381, 889, 1017
Bsp1407I TGTACA 2 cut(s) 571, 1117
Bsp19I CCATGG 1 cut(s) 912
BspACI CCGC 6 cut(s) 592, 698, 941, 952, 1417, 1540
BspANI GGCC 1 cut(s) 1176
BspCNI CTCAG 4 cut(s) 358, 367, 1348, 1446
BspFNI CGCG 2 cut(s) 594, 698
BspLI GGNNCC 4 cut(s) 156, 273, 360, 792
BspPI GGATC 7 cut(s) 149, 162, 560, 785, 798, 1174, 1451
BspTNI GGTCTC 2 cut(s) 210, 755
BsrDI GCAATG 2 cut(s) 850, 1290
BsrGI TGTACA 2 cut(s) 571, 1117
BsrI ACTGG 3 cut(s) 275, 854, 1231
BssECI CCNNGG 6 cut(s) 143, 158, 912, 1096, 1377, 1518
BssT1I CCWWGG 2 cut(s) 912, 1518
Bst2UI CCWGG 5 cut(s) 145, 159, 930, 1097, 1378
Bst4CI ACNGT 3 cut(s) 385, 1046, 1490
Bst6I CTCTTC 1 cut(s) 437
BstAUI TGTACA 2 cut(s) 571, 1117
BstBAI YACGTR 2 cut(s) 1028, 1402
BstC8I GCNNGC 6 cut(s) 596, 678, 954, 1178, 1182, 1327
BstDEI CTNAG 4 cut(s) 345, 354, 1335, 1433
BstDSI CCRYGG 1 cut(s) 912
BstF5I GGATG 2 cut(s) 433, 925
BstFNI CGCG 2 cut(s) 594, 698
BstHHI GCGC 2 cut(s) 131, 1056
BstMAI GTCTC 3 cut(s) 210, 755, 1456
BstMWI GCNNNNNNNGC 6 cut(s) 461, 547, 776, 1079, 1147, 1392
BstNI CCWGG 5 cut(s) 145, 159, 930, 1097, 1378
BstNSI RCATGY 2 cut(s) 578, 662
BstSCI CCNGG 5 cut(s) 143, 157, 928, 1095, 1376
BstSLI GKGCMC 1 cut(s) 1017
BstSNI TACGTA 1 cut(s) 1028
BstUI CGCG 2 cut(s) 594, 698
BstV1I GCAGC 7 cut(s) 337, 439, 442, 866, 1060, 1395, 1407
BstV2I GAAGAC 1 cut(s) 156
BstX2I RGATCY 5 cut(s) 154, 790, 1166, 1429, 1480
BstXI CCANNNNNNTGG 2 cut(s) 151, 558
BstYI RGATCY 5 cut(s) 154, 790, 1166, 1429, 1480
BsuRI GGCC 1 cut(s) 1176
BtgI CCRYGG 1 cut(s) 912
BtgZI GCGATG 1 cut(s) 872
BtsCI GGATG 2 cut(s) 433, 925
BtsI GCAGTG 1 cut(s) 503
BtsIMutI CAGTG 3 cut(s) 503, 540, 1224
Cac8I GCNNGC 6 cut(s) 596, 678, 954, 1178, 1182, 1327
CfoI GCGC 2 cut(s) 131, 1056
Cfr13I GGNCC 1 cut(s) 1309
CseI GACGC 1 cut(s) 704
Csp6I GTAC 5 cut(s) 342, 572, 834, 1025, 1118
CviQI GTAC 5 cut(s) 342, 572, 834, 1025, 1118
DdeI CTNAG 4 cut(s) 345, 354, 1335, 1433
Eam1104I CTCTTC 1 cut(s) 437
EarI CTCTTC 1 cut(s) 437
Eco105I TACGTA 1 cut(s) 1028
Eco130I CCWWGG 2 cut(s) 912, 1518
Eco24I GRGCYC 2 cut(s) 381, 889
Eco31I GGTCTC 2 cut(s) 210, 755
Eco47I GGWCC 1 cut(s) 1309
Eco57I CTGAAG 2 cut(s) 1263, 1391
Eco88I CYCGRG 1 cut(s) 681
EcoRII CCWGG 5 cut(s) 143, 157, 928, 1095, 1376
EcoT14I CCWWGG 2 cut(s) 912, 1518
EcoT38I GRGCYC 2 cut(s) 381, 889
ErhI CCWWGG 2 cut(s) 912, 1518
FauI CCCGC 2 cut(s) 934, 945
Fnu4HI GCNGC 8 cut(s) 351, 453, 456, 592, 855, 1074, 1384, 1396
FokI GGATG 2 cut(s) 440, 932
FriOI GRGCYC 2 cut(s) 381, 889
Fsp4HI GCNGC 8 cut(s) 351, 453, 456, 592, 855, 1074, 1384, 1396
FspBI CTAG 1 cut(s) 1484
GlaI GCGC 2 cut(s) 130, 1055
GluI GCNGC 8 cut(s) 351, 453, 456, 592, 855, 1074, 1384, 1396
GsaI CCCAGC 3 cut(s) 49, 783, 831
GsuI CTGGAG 2 cut(s) 194, 1191
HaeIII GGCC 1 cut(s) 1176
HapII CCGG 1 cut(s) 237
HgaI GACGC 1 cut(s) 704
HhaI GCGC 2 cut(s) 131, 1056
Hin6I GCGC 2 cut(s) 129, 1054
HinP1I GCGC 2 cut(s) 129, 1054
HindIII AAGCTT 3 cut(s) 286, 674, 768
HpaII CCGG 1 cut(s) 237
HphI GGTGA 7 cut(s) 168, 251, 526, 944, 986, 1026, 1435
Hpy166II GTNNAC 4 cut(s) 664, 1015, 1216, 1309
Hpy188I TCNGA 9 cut(s) 20, 262, 373, 712, 1135, 1269, 1338, 1436, 1456
Hpy188III TCNNGA 8 cut(s) 211, 356, 683, 822, 1170, 1460, 1484, 1498
Hpy8I GTNNAC 4 cut(s) 664, 1015, 1216, 1309
Hpy99I CGWCG 1 cut(s) 897
HpyAV CCTTC 3 cut(s) 372, 625, 715
HpyCH4III ACNGT 3 cut(s) 385, 1046, 1490
HpyCH4IV ACGT 3 cut(s) 892, 1027, 1401
HpyCH4V TGCA 8 cut(s) 314, 458, 496, 578, 817, 868, 1015, 1346
HpyF10VI GCNNNNNNNGC 6 cut(s) 461, 547, 776, 1079, 1147, 1392
HpyF3I CTNAG 4 cut(s) 345, 354, 1335, 1433
HpySE526I ACGT 3 cut(s) 892, 1027, 1401
HspAI GCGC 2 cut(s) 129, 1054
LmnI GCTCC 4 cut(s) 358, 538, 784, 824
Lsp1109I GCAGC 7 cut(s) 337, 439, 442, 866, 1060, 1395, 1407
LweI GCATC 1 cut(s) 32
MaeI CTAG 1 cut(s) 1484
MaeII ACGT 3 cut(s) 892, 1027, 1401
MaeIII GTNAC 3 cut(s) 121, 446, 1084
MboII GAAGA 8 cut(s) 156, 159, 249, 454, 514, 753, 1351, 1544
MfeI CAATTG 2 cut(s) 228, 459
MflI RGATCY 5 cut(s) 154, 790, 1166, 1429, 1480
MhlI GDGCHC 3 cut(s) 381, 889, 1017
MluCI AATT 7 cut(s) 83, 138, 228, 459, 647, 1122, 1441
MlyI GAGTC 3 cut(s) 454, 1081, 1327
MmeI TCCRAC 2 cut(s) 501, 684
MroXI GAANNNNTTC 1 cut(s) 745
MseI TTAA 3 cut(s) 399, 483, 1125
MslI CAYNNNNRTG 1 cut(s) 869
MspI CCGG 1 cut(s) 237
MspR9I CCNGG 5 cut(s) 145, 159, 930, 1097, 1378
MunI CAATTG 2 cut(s) 228, 459
Mva1269I GAATGC 1 cut(s) 1348
MvaI CCWGG 5 cut(s) 145, 159, 930, 1097, 1378
MvnI CGCG 2 cut(s) 594, 698
MwoI GCNNNNNNNGC 6 cut(s) 461, 547, 776, 1079, 1147, 1392
NcoI CCATGG 1 cut(s) 912
NlaIV GGNNCC 4 cut(s) 156, 273, 360, 792
NmuCI GTSAC 3 cut(s) 121, 446, 1084
NspI RCATGY 2 cut(s) 578, 662
PctI GAATGC 1 cut(s) 1348
PdmI GAANNNNTTC 1 cut(s) 745
PfeI GAWTC 8 cut(s) 77, 393, 415, 707, 713, 945, 1130, 1463
PkrI GCNGC 8 cut(s) 352, 454, 457, 593, 856, 1075, 1385, 1397
PleI GAGTC 3 cut(s) 453, 1081, 1327
PpsI GAGTC 3 cut(s) 453, 1081, 1327
Ppu21I YACGTR 2 cut(s) 1028, 1402
PshBI ATTAAT 1 cut(s) 1125
Psp6I CCWGG 5 cut(s) 143, 157, 928, 1095, 1376
PspFI CCCAGC 3 cut(s) 45, 779, 827
PspGI CCWGG 5 cut(s) 143, 157, 928, 1095, 1376
PspN4I GGNNCC 4 cut(s) 156, 273, 360, 792
PspPI GGNCC 1 cut(s) 1309
PsuI RGATCY 5 cut(s) 154, 790, 1166, 1429, 1480
RsaI GTAC 5 cut(s) 343, 573, 835, 1026, 1119
RsaNI GTAC 5 cut(s) 342, 572, 834, 1025, 1118
RseI CAYNNNNRTG 1 cut(s) 869
SaqAI TTAA 3 cut(s) 399, 483, 1125
SatI GCNGC 8 cut(s) 351, 453, 456, 592, 855, 1074, 1384, 1396
Sau96I GGNCC 1 cut(s) 1309
SchI GAGTC 3 cut(s) 454, 1081, 1327
ScrFI CCNGG 5 cut(s) 145, 159, 930, 1097, 1378
SduI GDGCHC 3 cut(s) 381, 889, 1017
SfaNI GCATC 1 cut(s) 32
SinI GGWCC 1 cut(s) 1309
SmiMI CAYNNNNRTG 1 cut(s) 869
SmlI CTYRAG 3 cut(s) 731, 1472, 1498
SmoI CTYRAG 3 cut(s) 731, 1472, 1498
SnaBI TACGTA 1 cut(s) 1028
Sse9I AATT 7 cut(s) 83, 138, 228, 459, 647, 1122, 1441
SsiI CCGC 6 cut(s) 592, 698, 941, 952, 1417, 1540
SspI AATATT 1 cut(s) 1352
SspMI CTAG 1 cut(s) 1484
StyD4I CCNGG 5 cut(s) 143, 157, 928, 1095, 1376
StyI CCWWGG 2 cut(s) 912, 1518
TaaI ACNGT 3 cut(s) 385, 1046, 1490
TaiI ACGT 3 cut(s) 895, 1030, 1404
TaqI TCGA 5 cut(s) 331, 365, 391, 413, 1146
TasI AATT 7 cut(s) 83, 138, 228, 459, 647, 1122, 1441
TatI WGTACW 2 cut(s) 571, 1117
TauI GCSGC 1 cut(s) 594
TfiI GAWTC 8 cut(s) 77, 393, 415, 707, 713, 945, 1130, 1463
Tru1I TTAA 3 cut(s) 399, 483, 1125
Tru9I TTAA 3 cut(s) 399, 483, 1125
TscAI CASTG 3 cut(s) 503, 540, 1231
TseFI GTSAC 3 cut(s) 121, 446, 1084
TseI GCWGC 7 cut(s) 350, 452, 455, 854, 1073, 1383, 1395
Tsp45I GTSAC 3 cut(s) 121, 446, 1084
TspRI CASTG 3 cut(s) 503, 540, 1231
VneI GTGCAC 1 cut(s) 1013
VpaK11BI GGWCC 1 cut(s) 1309
VspI ATTAAT 1 cut(s) 1125
XapI RAATTY 1 cut(s) 138
XbaI TCTAGA 1 cut(s) 1483
XceI RCATGY 2 cut(s) 578, 662
XmnI GAANNNNTTC 1 cut(s) 745
XspI CTAG 1 cut(s) 1484
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.