Rw3G006690

Peptide chain release factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0014362
Physical Location & Seq
Forward (+)
6248946 .. 6251733
2788 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G006690.1

Sequence Viewer

Length: 1545 bp
ATGTCATCTTTCCTGTTCAGAAAAAGAGCAAGCAGTAGTAGATGCTGGGGGGTTCTCTGCCGAAACCCTAAAGCTGAATCTCAATTTGGTAATCTCAATAAACCCCCAAAACCTCAAAATGTCACAAGCGCAGTTGAAATTCCCAGGATTTTGGATCCTGGGTCTTCTTCATTTTCACCCAAAACCTCTAAAACACTATCTGGGTCTCTCCAGAACATCAAAAACTCAATTGCTTCCGGTGAAAGTTTTGGGCTTTATTCTGATTTGGGGGTTCCAGTTGTGAGAAGCTTATGTGGGTTTCATGGTGGAATTGCAACTTGTAGCTTGGTTCGATTTTATGGGGCTCAGGCAGCTCAGGAGCCTTCGACCGCTGATGGGCTCACAGTTGATCGAATCCTTAATAGTGAGTGGTCAATTATGGCAGAGGATGAGAGTGATTGGAAGAGTCACGCTGCTGCAATTGCCCAATCAGTTCATCTTATTAAGAAGCGTTTGCAGTGGAAGAAGTTGCTGGTGAGGTTGGATATGTTATCAGTGGAGCTGAATAAACCAGACCTTTGGAATGATCCTGTACATGCCGGGAAGATAAGTCGCGAGCATGGTTCACTGATGGGAAAAATGAAAGAAGTGAAGGCATTTGAGCAGGAATTGCTTGAGCATGTGGACATGATAAAGCTTGCTCGGGAGGAGAATGATGCAGAGTTGGAATCGGAATCCATGAAGGCTTTGCTTGAGATGAGAAGAACTTCAAAGGAGAAAGAGACCCAAGCTTTGTTAGCTGGGGAGCAGGATCCTTGCTCATGTTATATAGAGGTGCAATCGGGAGCTGGGGGTACAGAGAGCAACGACTGGGCAGCGATGGTCATGCAGATGTATAAAATGTGGGCTCAACGCCATGGATATAAATTGACCGTGGTGGATGAAATGCCTGGTGAGATTGCGGGAATCAAGCGGGCAACTATCAAAGTTGATGGTGAATATGCTTTTGGATATGCCAAAGCTGAAGTAGGAGTGCACAGGTTGGTACGTATCTCACCTTTTGACAGTAATAAGCGCAGACATACTTCATTTGCTGCTGTCGCTGTGACTCCAATCCTGGGTGATGTATCTGCTCATGTACAAATTAATGAATCTGATCTGCGTATTGAGCGTTTTCGTTCAGGGGGATCTGGAGGCCAGCACGCTAATACGACTGATAGTGCTGTGAGGATTGTTCACATTCCCACTGGAATAACTGCCACTTGTCAAAATGAAAGATCACAACATCAGAATAAGGCTTCAGCAATGGCTGTTATCCAAGCAAGAGTAGACCAGCTTGAAATGGCACGCCAGACTCAGAAGAATGCACAATATTCACAATCTCTTACTGATATAACCTGGGGCAGCCAGATACGCAGCTACGTGCTCCAGCCATACCGCATGGTGAAAGATCTTAGAACCAATTATGAAGTCTCTGATCCTGATTCTGTCCTTGAAGGAGATCTAGACGGTTTTATCTTGAGTTATTTATCAGCTTCCTTGGATAAAGATGAAGATGACCGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PCRF RF-1

Protein Analysis

514

Amino Acids

56.96

Weight (kDa)

6.52

Isoelectric Point (pI)

45.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCRF PF03462 173 - 360 2.9e-51 PCRF domain
RF-1 PF00472 372 - 479 6.5e-36 RF-1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014362)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G56350
fragaria_vesca FvH4_6g07390
malus_domestica MD04G1193600.v1.1 MD12G1206300.v1.1
prunus_persica Prupe.6G313600_v2.0.a1 Prupe.6G313600_v2.0.a1
pyrus_communis pycom12g19420
rosa_chinensis RchiOBHm_Chr3g0456991
rosa_laevigata RLG00000025255
rosa_multiflora Rmu_co8479901.1_g000001
rosa_roxburghii Rroxscaffold_6G00422290
rosa_rugosa Rorug03G0018100
rosa_samantha Rh3AG077900 Rh3BG079900 Rh3CG079800 Rh3DG081600
rosa_wichuraiana Rw3G006690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1308
AccII CGCG 1 cut(s) 594
AciI CCGC 5 cut(s) 369, 941, 952, 1417, 1540
AclWI GGATC 7 cut(s) 149, 162, 560, 785, 798, 1174, 1451
AcsI RAATTY 1 cut(s) 138
AcuI CTGAAG 2 cut(s) 1023, 1263
AfaI GTAC 4 cut(s) 573, 835, 1026, 1119
AfiI CCNNNNNNNGG 2 cut(s) 375, 1097
AgsI TTSAA 4 cut(s) 137, 750, 1319, 1475
AjnI CCWGG 5 cut(s) 143, 157, 928, 1095, 1376
AjuI GAANNNNNNNTTGG 4 cut(s) 69, 101, 969, 1001
Alw21I GWGCWC 2 cut(s) 1017, 1407
Alw26I GTCTC 3 cut(s) 210, 755, 1456
Alw44I GTGCAC 1 cut(s) 1013
AlwI GGATC 7 cut(s) 149, 162, 560, 785, 798, 1174, 1451
Ama87I CYCGRG 1 cut(s) 681
AoxI GGCC 1 cut(s) 1174
ApaLI GTGCAC 1 cut(s) 1013
ApeKI GCWGC 7 cut(s) 350, 452, 455, 854, 1073, 1383, 1395
ApoI RAATTY 1 cut(s) 138
ArsI GACNNNNNNTTYG 2 cut(s) 754, 786
AseI ATTAAT 1 cut(s) 1125
Asp700I GAANNNNTTC 1 cut(s) 745
AspLEI GCGC 2 cut(s) 131, 1056
AsuC2I CCSGG 1 cut(s) 580
AsuHPI GGTGA 8 cut(s) 168, 251, 526, 944, 986, 1026, 1112, 1435
AvaI CYCGRG 1 cut(s) 681
BaeGI GKGCMC 1 cut(s) 1017
BamHI GGATCC 2 cut(s) 154, 790
BanII GRGCYC 3 cut(s) 346, 381, 889
BbsI GAAGAC 1 cut(s) 156
Bbv12I GWGCWC 2 cut(s) 1017, 1407
BbvI GCAGC 7 cut(s) 362, 439, 442, 866, 1060, 1395, 1407
BccI CCATC 4 cut(s) 368, 604, 853, 965
BcgI CGANNNNNNTGC 2 cut(s) 847, 881
BciT130I CCWGG 5 cut(s) 145, 159, 930, 1097, 1378
BcnI CCSGG 1 cut(s) 580
BcoDI GTCTC 3 cut(s) 210, 755, 1456
BfaI CTAG 1 cut(s) 1484
BglII AGATCT 2 cut(s) 1429, 1480
BisI GCNGC 7 cut(s) 351, 453, 456, 855, 1074, 1384, 1396
BlsI GCNGC 7 cut(s) 352, 454, 457, 856, 1075, 1385, 1397
Bme1390I CCNGG 6 cut(s) 145, 159, 580, 930, 1097, 1378
BmeT110I CYCGRG 1 cut(s) 681
BmiI GGNNCC 4 cut(s) 156, 273, 360, 792
BmrFI CCNGG 6 cut(s) 145, 159, 580, 930, 1097, 1378
BmrI ACTGGG 1 cut(s) 859
BmsI GCATC 2 cut(s) 32, 685
BmuI ACTGGG 1 cut(s) 859
BpiI GAAGAC 1 cut(s) 156
BpmI CTGGAG 3 cut(s) 194, 1191, 1391
Bpu10I CCTNAGC 2 cut(s) 345, 354
BpuEI CTTGAG 3 cut(s) 674, 752, 1519
BpuMI CCSGG 1 cut(s) 580
BsaAI YACGTR 2 cut(s) 1028, 1402
BsaI GGTCTC 2 cut(s) 210, 755
BsaJI CCNNGG 7 cut(s) 143, 158, 895, 912, 1096, 1377, 1518
BsaWI WCCGGW 1 cut(s) 236
Bsc4I CCNNNNNNNGG 2 cut(s) 375, 1097
Bse1I ACTGG 3 cut(s) 275, 854, 1231
Bse3DI GCAATG 1 cut(s) 1290
BseBI CCWGG 5 cut(s) 145, 159, 930, 1097, 1378
BseDI CCNNGG 7 cut(s) 143, 158, 895, 912, 1096, 1377, 1518
BseGI GGATG 2 cut(s) 433, 925
BseLI CCNNNNNNNGG 2 cut(s) 375, 1097
BseMI GCAATG 1 cut(s) 1290
BseMII CTCAG 3 cut(s) 359, 368, 1349
BseNI ACTGG 3 cut(s) 275, 854, 1231
BseRI GAGGAG 1 cut(s) 701
BseSI GKGCMC 1 cut(s) 1017
BseXI GCAGC 7 cut(s) 362, 439, 442, 866, 1060, 1395, 1407
BseYI CCCAGC 3 cut(s) 45, 779, 827
Bsh1236I CGCG 1 cut(s) 594
Bsh1285I CGRYCG 1 cut(s) 369
BshFI GGCC 1 cut(s) 1176
BsiEI CGRYCG 1 cut(s) 369
BsiHKAI GWGCWC 2 cut(s) 1017, 1407
BsiHKCI CYCGRG 1 cut(s) 681
BsiSI CCGG 2 cut(s) 237, 579
BslI CCNNNNNNNGG 2 cut(s) 375, 1097
BsmAI GTCTC 3 cut(s) 210, 755, 1456
BsmI GAATGC 1 cut(s) 1348
BsnI GGCC 1 cut(s) 1176
Bso31I GGTCTC 2 cut(s) 210, 755
BsoBI CYCGRG 1 cut(s) 681
Bsp1286I GDGCHC 5 cut(s) 346, 381, 889, 1017, 1407
Bsp1407I TGTACA 2 cut(s) 571, 1117
Bsp19I CCATGG 1 cut(s) 895
Bsp68I TCGCGA 1 cut(s) 594
BspACI CCGC 5 cut(s) 369, 941, 952, 1417, 1540
BspANI GGCC 1 cut(s) 1176
BspCNI CTCAG 3 cut(s) 358, 367, 1348
BspFNI CGCG 1 cut(s) 594
BspLI GGNNCC 4 cut(s) 156, 273, 360, 792
BspPI GGATC 7 cut(s) 149, 162, 560, 785, 798, 1174, 1451
BspTNI GGTCTC 2 cut(s) 210, 755
BsrDI GCAATG 1 cut(s) 1290
BsrGI TGTACA 2 cut(s) 571, 1117
BsrI ACTGG 3 cut(s) 275, 854, 1231
BssECI CCNNGG 7 cut(s) 143, 158, 895, 912, 1096, 1377, 1518
BssT1I CCWWGG 2 cut(s) 895, 1518
Bst2UI CCWGG 5 cut(s) 145, 159, 930, 1097, 1378
Bst4CI ACNGT 4 cut(s) 385, 913, 1046, 1490
Bst6I CTCTTC 1 cut(s) 437
BstAPI GCANNNNNTGC 1 cut(s) 649
BstAUI TGTACA 2 cut(s) 571, 1117
BstBAI YACGTR 2 cut(s) 1028, 1402
BstC8I GCNNGC 7 cut(s) 31, 596, 678, 954, 1178, 1182, 1327
BstDEI CTNAG 4 cut(s) 345, 354, 1335, 1433
BstDSI CCRYGG 2 cut(s) 895, 912
BstF5I GGATG 2 cut(s) 433, 925
BstFNI CGCG 1 cut(s) 594
BstHHI GCGC 2 cut(s) 131, 1056
BstMAI GTCTC 3 cut(s) 210, 755, 1456
BstMCI CGRYCG 1 cut(s) 369
BstMWI GCNNNNNNNGC 7 cut(s) 350, 461, 649, 776, 1079, 1147, 1392
BstNI CCWGG 5 cut(s) 145, 159, 930, 1097, 1378
BstNSI RCATGY 2 cut(s) 578, 662
BstSCI CCNGG 6 cut(s) 143, 157, 578, 928, 1095, 1376
BstSLI GKGCMC 1 cut(s) 1017
BstSNI TACGTA 1 cut(s) 1028
BstUI CGCG 1 cut(s) 594
BstV1I GCAGC 7 cut(s) 362, 439, 442, 866, 1060, 1395, 1407
BstV2I GAAGAC 1 cut(s) 156
BstX2I RGATCY 5 cut(s) 154, 790, 1166, 1429, 1480
BstXI CCANNNNNNTGG 2 cut(s) 151, 558
BstYI RGATCY 5 cut(s) 154, 790, 1166, 1429, 1480
BsuRI GGCC 1 cut(s) 1176
BtgI CCRYGG 2 cut(s) 895, 912
BtgZI GCGATG 1 cut(s) 872
BtsCI GGATG 2 cut(s) 433, 925
BtsI GCAGTG 1 cut(s) 503
BtsIMutI CAGTG 4 cut(s) 503, 540, 605, 1224
BtuMI TCGCGA 1 cut(s) 594
Cac8I GCNNGC 7 cut(s) 31, 596, 678, 954, 1178, 1182, 1327
CfoI GCGC 2 cut(s) 131, 1056
Csp6I GTAC 4 cut(s) 572, 834, 1025, 1118
CviQI GTAC 4 cut(s) 572, 834, 1025, 1118
DdeI CTNAG 4 cut(s) 345, 354, 1335, 1433
Eam1104I CTCTTC 1 cut(s) 437
EarI CTCTTC 1 cut(s) 437
Eco105I TACGTA 1 cut(s) 1028
Eco130I CCWWGG 2 cut(s) 895, 1518
Eco24I GRGCYC 3 cut(s) 346, 381, 889
Eco31I GGTCTC 2 cut(s) 210, 755
Eco57I CTGAAG 2 cut(s) 1023, 1263
Eco88I CYCGRG 1 cut(s) 681
EcoRII CCWGG 5 cut(s) 143, 157, 928, 1095, 1376
EcoT14I CCWWGG 2 cut(s) 895, 1518
EcoT38I GRGCYC 3 cut(s) 346, 381, 889
ErhI CCWWGG 2 cut(s) 895, 1518
FauI CCCGC 2 cut(s) 934, 945
FblI GTMKAC 1 cut(s) 1308
Fnu4HI GCNGC 7 cut(s) 351, 453, 456, 855, 1074, 1384, 1396
FokI GGATG 2 cut(s) 440, 932
FriOI GRGCYC 3 cut(s) 346, 381, 889
Fsp4HI GCNGC 7 cut(s) 351, 453, 456, 855, 1074, 1384, 1396
FspBI CTAG 1 cut(s) 1484
GlaI GCGC 2 cut(s) 130, 1055
GluI GCNGC 7 cut(s) 351, 453, 456, 855, 1074, 1384, 1396
GsaI CCCAGC 3 cut(s) 49, 783, 831
GsuI CTGGAG 3 cut(s) 194, 1191, 1391
HaeIII GGCC 1 cut(s) 1176
HapII CCGG 2 cut(s) 237, 579
HhaI GCGC 2 cut(s) 131, 1056
Hin6I GCGC 2 cut(s) 129, 1054
HinP1I GCGC 2 cut(s) 129, 1054
HindIII AAGCTT 3 cut(s) 286, 674, 768
HpaII CCGG 2 cut(s) 237, 579
HphI GGTGA 8 cut(s) 168, 251, 526, 944, 986, 1026, 1112, 1435
Hpy166II GTNNAC 5 cut(s) 605, 664, 1015, 1216, 1309
Hpy188I TCNGA 7 cut(s) 20, 262, 712, 1135, 1269, 1338, 1456
Hpy188III TCNNGA 9 cut(s) 211, 356, 593, 683, 822, 1170, 1460, 1484, 1498
Hpy8I GTNNAC 5 cut(s) 605, 664, 1015, 1216, 1309
HpyAV CCTTC 4 cut(s) 372, 625, 715, 1469
HpyCH4III ACNGT 4 cut(s) 385, 913, 1046, 1490
HpyCH4IV ACGT 2 cut(s) 1027, 1401
HpyCH4V TGCA 8 cut(s) 314, 458, 496, 698, 817, 868, 1015, 1346
HpyF10VI GCNNNNNNNGC 7 cut(s) 350, 461, 649, 776, 1079, 1147, 1392
HpyF3I CTNAG 4 cut(s) 345, 354, 1335, 1433
HpySE526I ACGT 2 cut(s) 1027, 1401
HspAI GCGC 2 cut(s) 129, 1054
LmnI GCTCC 5 cut(s) 358, 538, 784, 824, 1410
Lsp1109I GCAGC 7 cut(s) 362, 439, 442, 866, 1060, 1395, 1407
LweI GCATC 2 cut(s) 32, 685
MaeI CTAG 1 cut(s) 1484
MaeII ACGT 2 cut(s) 1027, 1401
MaeIII GTNAC 3 cut(s) 121, 446, 1084
MboII GAAGA 8 cut(s) 156, 159, 454, 514, 595, 753, 1351, 1544
MfeI CAATTG 2 cut(s) 228, 459
MflI RGATCY 5 cut(s) 154, 790, 1166, 1429, 1480
MhlI GDGCHC 5 cut(s) 346, 381, 889, 1017, 1407
MlyI GAGTC 3 cut(s) 454, 1081, 1327
MmeI TCCRAC 2 cut(s) 501, 684
MnlI CCTC 8 cut(s) 123, 196, 418, 510, 679, 805, 1166, 1200
MroXI GAANNNNTTC 1 cut(s) 745
MseI TTAA 3 cut(s) 399, 483, 1125
MslI CAYNNNNRTG 1 cut(s) 869
MspA1I CMGCKG 1 cut(s) 371
MspI CCGG 2 cut(s) 237, 579
MspR9I CCNGG 6 cut(s) 145, 159, 580, 930, 1097, 1378
MunI CAATTG 2 cut(s) 228, 459
Mva1269I GAATGC 1 cut(s) 1348
MvaI CCWGG 5 cut(s) 145, 159, 930, 1097, 1378
MvnI CGCG 1 cut(s) 594
MwoI GCNNNNNNNGC 7 cut(s) 350, 461, 649, 776, 1079, 1147, 1392
NciI CCSGG 1 cut(s) 580
NcoI CCATGG 1 cut(s) 895
NlaIV GGNNCC 4 cut(s) 156, 273, 360, 792
NmuCI GTSAC 3 cut(s) 121, 446, 1084
NruI TCGCGA 1 cut(s) 594
NspI RCATGY 2 cut(s) 578, 662
PctI GAATGC 1 cut(s) 1348
PdmI GAANNNNTTC 1 cut(s) 745
PfeI GAWTC 7 cut(s) 77, 393, 707, 713, 945, 1130, 1463
PkrI GCNGC 7 cut(s) 352, 454, 457, 856, 1075, 1385, 1397
PleI GAGTC 3 cut(s) 453, 1081, 1327
PpsI GAGTC 3 cut(s) 453, 1081, 1327
Ppu21I YACGTR 2 cut(s) 1028, 1402
PshBI ATTAAT 1 cut(s) 1125
Psp6I CCWGG 5 cut(s) 143, 157, 928, 1095, 1376
PspFI CCCAGC 3 cut(s) 45, 779, 827
PspGI CCWGG 5 cut(s) 143, 157, 928, 1095, 1376
PspN4I GGNNCC 4 cut(s) 156, 273, 360, 792
PsuI RGATCY 5 cut(s) 154, 790, 1166, 1429, 1480
RruI TCGCGA 1 cut(s) 594
RsaI GTAC 4 cut(s) 573, 835, 1026, 1119
RsaNI GTAC 4 cut(s) 572, 834, 1025, 1118
RseI CAYNNNNRTG 1 cut(s) 869
SaqAI TTAA 3 cut(s) 399, 483, 1125
SatI GCNGC 7 cut(s) 351, 453, 456, 855, 1074, 1384, 1396
SchI GAGTC 3 cut(s) 454, 1081, 1327
ScrFI CCNGG 6 cut(s) 145, 159, 580, 930, 1097, 1378
SduI GDGCHC 5 cut(s) 346, 381, 889, 1017, 1407
SfaNI GCATC 2 cut(s) 32, 685
SmiMI CAYNNNNRTG 1 cut(s) 869
SmlI CTYRAG 3 cut(s) 653, 731, 1498
SmoI CTYRAG 3 cut(s) 653, 731, 1498
SnaBI TACGTA 1 cut(s) 1028
SsiI CCGC 5 cut(s) 369, 941, 952, 1417, 1540
SspI AATATT 1 cut(s) 1352
SspMI CTAG 1 cut(s) 1484
StyD4I CCNGG 6 cut(s) 143, 157, 578, 928, 1095, 1376
StyI CCWWGG 2 cut(s) 895, 1518
TaaI ACNGT 4 cut(s) 385, 913, 1046, 1490
TaiI ACGT 2 cut(s) 1030, 1404
TaqI TCGA 3 cut(s) 331, 365, 391
TatI WGTACW 2 cut(s) 571, 1117
TfiI GAWTC 7 cut(s) 77, 393, 707, 713, 945, 1130, 1463
Tru1I TTAA 3 cut(s) 399, 483, 1125
Tru9I TTAA 3 cut(s) 399, 483, 1125
TscAI CASTG 4 cut(s) 503, 540, 612, 1231
TseFI GTSAC 3 cut(s) 121, 446, 1084
TseI GCWGC 7 cut(s) 350, 452, 455, 854, 1073, 1383, 1395
Tsp45I GTSAC 3 cut(s) 121, 446, 1084
TspRI CASTG 4 cut(s) 503, 540, 612, 1231
VneI GTGCAC 1 cut(s) 1013
VspI ATTAAT 1 cut(s) 1125
XapI RAATTY 1 cut(s) 138
XbaI TCTAGA 1 cut(s) 1483
XceI RCATGY 2 cut(s) 578, 662
XmiI GTMKAC 1 cut(s) 1308
XmnI GAANNNNTTC 1 cut(s) 745
XspI CTAG 1 cut(s) 1484
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.