AT1G60660

Cytochrome b5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0010898
Physical Location & Seq
Reverse (-)
22342290 .. 22343269
980 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G60660.1

Sequence Viewer

Length: 366 bp
ATGATAGCTGTGATCGGATTGCTTCTGGGTTTTCTAGTATCAGCATTGTTCTTAATCCAAGGAAAGCGAAGAAGAACAAACGACAATCAAGAGAAGAAGAGATCTAGCAGTGAGCCTGTAGAAGATGTGGTAAGACCAAAAAGTTACAGCAAGAGTGAAGTCGCAGTACATAACAAAAGGAACGATTGTTGGATCATAATCAAAGATAAAGTCTATGATATTACTTCTTATGTTGAAGAACATCCTGGTGGTGACGCTATTCTTGATCATGCTGGTGATGATTCTACTGATGGCTTCTTCGGACCTCAACACGCCACTCGTGTTTTCGACATGATTGAAGATTTCTACATCGGGGAACTTCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyt-b5

Protein Analysis

121

Amino Acids

13.71

Weight (kDa)

5.46

Isoelectric Point (pI)

64.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyt-b5 PF00173 50 - 120 2.1e-24 Cytochrome b5-like Heme/Steroid binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010898)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G60660
fragaria_vesca FvH4_1g06100 FvH4_1g06100 FvH4_1g06100
malus_domestica MD02G1065500.v1.1
prunus_persica Prupe.7G220000_v2.0.a1 Prupe.7G220000_v2.0.a1
pyrus_communis pycom02g05160
rosa_chinensis RchiOBHm_Chr2g0091851
rosa_laevigata RLG00000016257
rosa_multiflora Rmu_co8418845.1_g000001
rosa_roxburghii Rroxscaffold_2G00149670 Rroxscaffold_2G00149680
rosa_rugosa Rorug02G0021500
rosa_samantha Rh2AG066300 Rh2AG066500 Rh2BG065700 Rh2CG067100 Rh2DG065500
rosa_wichuraiana Rw2G005440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 200
AfaI GTAC 1 cut(s) 168
AgsI TTSAA 2 cut(s) 236, 338
AjnI CCWGG 1 cut(s) 244
AluBI AGCT 1 cut(s) 8
AluI AGCT 1 cut(s) 8
AlwI GGATC 1 cut(s) 200
ArsI GACNNNNNNTTYG 2 cut(s) 195, 227
AspS9I GGNCC 1 cut(s) 302
AsuHPI GGTGA 2 cut(s) 263, 287
AvaII GGWCC 1 cut(s) 302
BarI GAAGNNNNNNTAC 2 cut(s) 150, 182
BauI CACGAG 1 cut(s) 318
BccI CCATC 1 cut(s) 284
BciT130I CCWGG 1 cut(s) 246
BclI TGATCA 1 cut(s) 265
BfaI CTAG 2 cut(s) 35, 105
BfmI CTRYAG 1 cut(s) 117
BglII AGATCT 1 cut(s) 101
Bme1390I CCNGG 1 cut(s) 246
Bme18I GGWCC 1 cut(s) 302
BmgT120I GGNCC 1 cut(s) 302
BmrFI CCNGG 1 cut(s) 246
BsaBI GATNNNNATC 1 cut(s) 197
BsaJI CCNNGG 1 cut(s) 58
Bse8I GATNNNNATC 1 cut(s) 197
BseBI CCWGG 1 cut(s) 246
BseDI CCNNGG 1 cut(s) 58
BseGI GGATG 1 cut(s) 241
BseJI GATNNNNATC 1 cut(s) 197
Bsp143I GATC 4 cut(s) 12, 101, 192, 265
BspPI GGATC 1 cut(s) 200
BssECI CCNNGG 1 cut(s) 58
BssMI GATC 4 cut(s) 12, 101, 192, 265
BssSI CACGAG 1 cut(s) 318
BssT1I CCWWGG 1 cut(s) 58
Bst2BI CACGAG 1 cut(s) 318
Bst2UI CCWGG 1 cut(s) 246
Bst6I CTCTTC 1 cut(s) 92
BstF5I GGATG 1 cut(s) 241
BstKTI GATC 4 cut(s) 15, 104, 195, 268
BstMBI GATC 4 cut(s) 12, 101, 192, 265
BstNI CCWGG 1 cut(s) 246
BstSCI CCNGG 1 cut(s) 244
BstSFI CTRYAG 1 cut(s) 117
BstX2I RGATCY 1 cut(s) 101
BstYI RGATCY 1 cut(s) 101
BtsCI GGATG 1 cut(s) 241
BtsI GCAGTG 1 cut(s) 115
BtsIMutI CAGTG 1 cut(s) 115
Cfr13I GGNCC 1 cut(s) 302
CseI GACGC 1 cut(s) 263
Csp6I GTAC 1 cut(s) 167
CviAII CATG 2 cut(s) 269, 331
CviJI RGCY 3 cut(s) 8, 115, 294
CviKI_1 RGCY 3 cut(s) 8, 115, 294
CviQI GTAC 1 cut(s) 167
DpnI GATC 4 cut(s) 14, 103, 194, 267
DpnII GATC 4 cut(s) 12, 101, 192, 265
Eam1104I CTCTTC 1 cut(s) 92
EarI CTCTTC 1 cut(s) 92
Eco130I CCWWGG 1 cut(s) 58
Eco47I GGWCC 1 cut(s) 302
EcoRII CCWGG 1 cut(s) 244
EcoT14I CCWWGG 1 cut(s) 58
ErhI CCWWGG 1 cut(s) 58
FaeI CATG 2 cut(s) 272, 334
FaiI YATR 6 cut(s) 171, 197, 216, 231, 270, 332
FatI CATG 2 cut(s) 268, 330
FbaI TGATCA 1 cut(s) 265
FokI GGATG 1 cut(s) 228
FspBI CTAG 2 cut(s) 35, 105
HgaI GACGC 1 cut(s) 263
Hin1II CATG 2 cut(s) 272, 334
HinfI GANTC 1 cut(s) 281
HphI GGTGA 2 cut(s) 263, 287
Hpy188I TCNGA 2 cut(s) 17, 302
Hpy188III TCNNGA 2 cut(s) 89, 263
Hsp92II CATG 2 cut(s) 272, 334
Ksp22I TGATCA 1 cut(s) 265
Kzo9I GATC 4 cut(s) 12, 101, 192, 265
LpnPI CCDG 5 cut(s) 11, 129, 231, 258, 258
MaeI CTAG 2 cut(s) 35, 105
MaeIII GTNAC 2 cut(s) 143, 251
MalI GATC 4 cut(s) 14, 103, 194, 267
MboI GATC 4 cut(s) 12, 101, 192, 265
MboII GAAGA 8 cut(s) 81, 84, 106, 109, 134, 248, 289, 350
MflI RGATCY 1 cut(s) 101
MmeI TCCRAC 1 cut(s) 170
MnlI CCTC 1 cut(s) 315
MseI TTAA 1 cut(s) 53
MslI CAYNNNNRTG 2 cut(s) 246, 273
MspR9I CCNGG 1 cut(s) 246
MvaI CCWGG 1 cut(s) 246
NdeII GATC 4 cut(s) 12, 101, 192, 265
NlaIII CATG 2 cut(s) 272, 334
NmuCI GTSAC 1 cut(s) 251
PfeI GAWTC 1 cut(s) 281
Psp6I CCWGG 1 cut(s) 244
PspGI CCWGG 1 cut(s) 244
PspPI GGNCC 1 cut(s) 302
PsuI RGATCY 1 cut(s) 101
RsaI GTAC 1 cut(s) 168
RsaNI GTAC 1 cut(s) 167
RseI CAYNNNNRTG 2 cut(s) 246, 273
SaqAI TTAA 1 cut(s) 53
Sau3AI GATC 4 cut(s) 12, 101, 192, 265
Sau96I GGNCC 1 cut(s) 302
ScrFI CCNGG 1 cut(s) 246
SetI ASST 2 cut(s) 10, 307
SfcI CTRYAG 1 cut(s) 117
SinI GGWCC 1 cut(s) 302
SmiMI CAYNNNNRTG 2 cut(s) 246, 273
SspMI CTAG 2 cut(s) 35, 105
StyD4I CCNGG 1 cut(s) 244
StyI CCWWGG 1 cut(s) 58
TaqI TCGA 1 cut(s) 327
TatI WGTACW 1 cut(s) 166
TfiI GAWTC 1 cut(s) 281
Tru1I TTAA 1 cut(s) 53
Tru9I TTAA 1 cut(s) 53
TscAI CASTG 1 cut(s) 115
TseFI GTSAC 1 cut(s) 251
Tsp45I GTSAC 1 cut(s) 251
TspDTI ATGAA 1 cut(s) 350
TspRI CASTG 1 cut(s) 115
VpaK11BI GGWCC 1 cut(s) 302
XspI CTAG 2 cut(s) 35, 105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.