RchiOBHm_Chr2g0091851

heme binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0010898
Physical Location & Seq
Forward (+)
5535483 .. 5537445
1963 bp
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UTR
Exon/CDS
Intron
PRQ46702

Sequence Viewer

Length: 363 bp
ATGATTCTAGCTGTGGTGGCATTGCTTCTGTGTGTCTTTTTAGGAGCTTTCGTTCTGATCCCATCTGGGAATACAAAAAACGTTAAGTCAAATGCTAAAGCTGAGAAGACGGCCAAGATATACTCTAAAGCTGAGGTTTCTTTGCATAATAAACGAACAGATTGTTGGGTGATTATCAAAAACAAGGTGTATGACCTTACACCATATGTAGAGGTACACCCGGGTGGTGATGCCATCCTCGCACACGCCGGGGATGATGCAACTGTTGGGTTTTATGGGCCACAGCATGCTAGCTTAGTCTATGATATGGTTCACGAATTCTATATTGGAGATCTGGAGCCTGGAGCCAGTGTAACTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyt-b5

Protein Analysis

120

Amino Acids

12.98

Weight (kDa)

6.16

Isoelectric Point (pI)

26.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyt-b5 PF00173 43 - 112 7.6e-22 Cytochrome b5-like Heme/Steroid binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010898)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G60660
fragaria_vesca FvH4_1g06100 FvH4_1g06100 FvH4_1g06100
malus_domestica MD02G1065500.v1.1
prunus_persica Prupe.7G220000_v2.0.a1 Prupe.7G220000_v2.0.a1
pyrus_communis pycom02g05160
rosa_chinensis RchiOBHm_Chr2g0091851
rosa_laevigata RLG00000016257
rosa_multiflora Rmu_co8418845.1_g000001
rosa_roxburghii Rroxscaffold_2G00149670 Rroxscaffold_2G00149680
rosa_rugosa Rorug02G0021500
rosa_samantha Rh2AG066300 Rh2AG066500 Rh2BG065700 Rh2CG067100 Rh2DG065500
rosa_wichuraiana Rw2G005440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 1 cut(s) 81
AclWI GGATC 1 cut(s) 52
AcoI YGGCCR 1 cut(s) 111
AcsI RAATTY 1 cut(s) 317
AfaI GTAC 1 cut(s) 216
AjnI CCWGG 1 cut(s) 340
AjuI GAANNNNNNNTTGG 4 cut(s) 148, 180, 309, 341
AleI CACNNNNGTG 1 cut(s) 222
AloI GAACNNNNNNTCC 2 cut(s) 36, 68
AluBI AGCT 5 cut(s) 11, 47, 101, 131, 294
AluI AGCT 5 cut(s) 11, 47, 101, 131, 294
AlwI GGATC 1 cut(s) 52
Ama87I CYCGRG 1 cut(s) 220
AoxI GGCC 2 cut(s) 111, 278
ApoI RAATTY 1 cut(s) 317
AspS9I GGNCC 1 cut(s) 278
AsuC2I CCSGG 3 cut(s) 221, 222, 250
AsuHPI GGTGA 2 cut(s) 181, 239
AsuNHI GCTAGC 1 cut(s) 290
AvaI CYCGRG 1 cut(s) 220
BbsI GAAGAC 1 cut(s) 113
BbvCI CCTCAGC 1 cut(s) 132
BccI CCATC 2 cut(s) 70, 242
BceAI ACGGC 1 cut(s) 126
BciT130I CCWGG 1 cut(s) 342
BcnI CCSGG 3 cut(s) 221, 222, 250
BfaI CTAG 2 cut(s) 8, 291
BglII AGATCT 1 cut(s) 331
Bme1390I CCNGG 4 cut(s) 221, 222, 250, 342
BmeT110I CYCGRG 1 cut(s) 220
BmgT120I GGNCC 1 cut(s) 278
BmiI GGNNCC 2 cut(s) 339, 346
BmrFI CCNGG 4 cut(s) 221, 222, 250, 342
BmsI GCATC 2 cut(s) 220, 247
BmtI GCTAGC 1 cut(s) 294
BpiI GAAGAC 1 cut(s) 113
BpmI CTGGAG 2 cut(s) 356, 363
Bpu10I CCTNAGC 1 cut(s) 132
BpuMI CCSGG 3 cut(s) 221, 222, 250
BsaJI CCNNGG 2 cut(s) 220, 249
BsaXI ACNNNNNCTCC 3 cut(s) 36, 66, 336
Bse1I ACTGG 1 cut(s) 348
Bse3DI GCAATG 1 cut(s) 20
BseBI CCWGG 1 cut(s) 342
BseDI CCNNGG 2 cut(s) 220, 249
BseGI GGATG 2 cut(s) 234, 259
BseMI GCAATG 1 cut(s) 20
BseMII CTCAG 2 cut(s) 93, 123
BseNI ACTGG 1 cut(s) 348
BshFI GGCC 2 cut(s) 113, 280
BsiHKCI CYCGRG 1 cut(s) 220
BsiSI CCGG 2 cut(s) 221, 249
BsnI GGCC 2 cut(s) 113, 280
BsoBI CYCGRG 1 cut(s) 220
Bsp143I GATC 2 cut(s) 57, 331
BspANI GGCC 2 cut(s) 113, 280
BspCNI CTCAG 2 cut(s) 94, 124
BspLI GGNNCC 2 cut(s) 339, 346
BspOI GCTAGC 1 cut(s) 294
BspPI GGATC 1 cut(s) 52
BsrDI GCAATG 1 cut(s) 20
BsrI ACTGG 1 cut(s) 348
BssECI CCNNGG 2 cut(s) 220, 249
BssMI GATC 2 cut(s) 57, 331
Bst2UI CCWGG 1 cut(s) 342
Bst4CI ACNGT 1 cut(s) 265
BstC8I GCNNGC 2 cut(s) 288, 292
BstDEI CTNAG 3 cut(s) 102, 132, 295
BstF5I GGATG 2 cut(s) 234, 259
BstKTI GATC 2 cut(s) 60, 334
BstMBI GATC 2 cut(s) 57, 331
BstMWI GCNNNNNNNGC 2 cut(s) 17, 239
BstNI CCWGG 1 cut(s) 342
BstNSI RCATGY 1 cut(s) 290
BstSCI CCNGG 4 cut(s) 219, 220, 248, 340
BstV2I GAAGAC 1 cut(s) 113
BstX2I RGATCY 1 cut(s) 331
BstYI RGATCY 1 cut(s) 331
BsuRI GGCC 2 cut(s) 113, 280
BtsCI GGATG 2 cut(s) 234, 259
BtsIMutI CAGTG 1 cut(s) 355
Cac8I GCNNGC 2 cut(s) 288, 292
Cfr13I GGNCC 1 cut(s) 278
Cfr9I CCCGGG 1 cut(s) 220
Csp6I GTAC 1 cut(s) 215
CviAII CATG 1 cut(s) 287
CviJI RGCY 9 cut(s) 11, 47, 101, 113, 131, 280, 294, 340, 347
CviKI_1 RGCY 9 cut(s) 11, 47, 101, 113, 131, 280, 294, 340, 347
CviQI GTAC 1 cut(s) 215
DdeI CTNAG 3 cut(s) 102, 132, 295
DpnI GATC 2 cut(s) 59, 333
DpnII GATC 2 cut(s) 57, 331
EaeI YGGCCR 1 cut(s) 111
Eco88I CYCGRG 1 cut(s) 220
EcoRI GAATTC 1 cut(s) 317
EcoRII CCWGG 1 cut(s) 340
FaeI CATG 1 cut(s) 290
FatI CATG 1 cut(s) 286
FauNDI CATATG 1 cut(s) 205
FokI GGATG 2 cut(s) 221, 266
FspBI CTAG 2 cut(s) 8, 291
GsuI CTGGAG 2 cut(s) 356, 363
HaeIII GGCC 2 cut(s) 113, 280
HapII CCGG 2 cut(s) 221, 249
Hin1II CATG 1 cut(s) 290
HinfI GANTC 1 cut(s) 4
HpaII CCGG 2 cut(s) 221, 249
HphI GGTGA 2 cut(s) 181, 239
Hpy166II GTNNAC 2 cut(s) 217, 313
Hpy188I TCNGA 1 cut(s) 57
Hpy188III TCNNGA 2 cut(s) 314, 335
Hpy8I GTNNAC 2 cut(s) 217, 313
HpyCH4III ACNGT 1 cut(s) 265
HpyCH4IV ACGT 1 cut(s) 81
HpyCH4V TGCA 2 cut(s) 145, 260
HpyF10VI GCNNNNNNNGC 2 cut(s) 17, 239
HpyF3I CTNAG 3 cut(s) 102, 132, 295
HpySE526I ACGT 1 cut(s) 81
Hsp92II CATG 1 cut(s) 290
Kzo9I GATC 2 cut(s) 57, 331
LmnI GCTCC 3 cut(s) 44, 337, 344
LpnPI CCDG 6 cut(s) 51, 234, 262, 320, 327, 354
LweI GCATC 2 cut(s) 220, 247
MaeI CTAG 2 cut(s) 8, 291
MaeII ACGT 1 cut(s) 81
MaeIII GTNAC 1 cut(s) 352
MalI GATC 2 cut(s) 59, 333
MboI GATC 2 cut(s) 57, 331
MboII GAAGA 1 cut(s) 118
MflI RGATCY 1 cut(s) 331
MluCI AATT 2 cut(s) 317, 358
MnlI CCTC 3 cut(s) 127, 205, 248
MseI TTAA 1 cut(s) 84
MslI CAYNNNNRTG 1 cut(s) 222
MspI CCGG 2 cut(s) 221, 249
MspR9I CCNGG 4 cut(s) 221, 222, 250, 342
MvaI CCWGG 1 cut(s) 342
MwoI GCNNNNNNNGC 2 cut(s) 17, 239
NciI CCSGG 3 cut(s) 221, 222, 250
NdeI CATATG 1 cut(s) 205
NdeII GATC 2 cut(s) 57, 331
NheI GCTAGC 1 cut(s) 290
NlaIII CATG 1 cut(s) 290
NlaIV GGNNCC 2 cut(s) 339, 346
NspI RCATGY 1 cut(s) 290
OliI CACNNNNGTG 1 cut(s) 222
PaeI GCATGC 1 cut(s) 290
PfeI GAWTC 1 cut(s) 4
Psp1406I AACGTT 1 cut(s) 81
Psp6I CCWGG 1 cut(s) 340
PspGI CCWGG 1 cut(s) 340
PspN4I GGNNCC 2 cut(s) 339, 346
PspPI GGNCC 1 cut(s) 278
PsuI RGATCY 1 cut(s) 331
RsaI GTAC 1 cut(s) 216
RsaNI GTAC 1 cut(s) 215
RseI CAYNNNNRTG 1 cut(s) 222
SaqAI TTAA 1 cut(s) 84
Sau3AI GATC 2 cut(s) 57, 331
Sau96I GGNCC 1 cut(s) 278
ScrFI CCNGG 4 cut(s) 221, 222, 250, 342
SfaNI GCATC 2 cut(s) 220, 247
SmaI CCCGGG 1 cut(s) 222
SmiMI CAYNNNNRTG 1 cut(s) 222
SphI GCATGC 1 cut(s) 290
Sse9I AATT 2 cut(s) 317, 358
SspMI CTAG 2 cut(s) 8, 291
StyD4I CCNGG 4 cut(s) 219, 220, 248, 340
TaaI ACNGT 1 cut(s) 265
TaiI ACGT 1 cut(s) 84
TasI AATT 2 cut(s) 317, 358
TfiI GAWTC 1 cut(s) 4
Tru1I TTAA 1 cut(s) 84
Tru9I TTAA 1 cut(s) 84
TscAI CASTG 1 cut(s) 355
TspMI CCCGGG 1 cut(s) 220
TspRI CASTG 1 cut(s) 355
XapI RAATTY 1 cut(s) 317
XceI RCATGY 1 cut(s) 290
XmaI CCCGGG 1 cut(s) 220
XspI CTAG 2 cut(s) 8, 291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.