MD02G1065500.v1.1

Belongs to the cytochrome b5 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0010898
Physical Location & Seq
Forward (+)
5308730 .. 5310682
1953 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1065500.v1.1.491

Sequence Viewer

Length: 348 bp
ATGATGATCGCCGCGGTGGCGCTGGTGGCGGTTATCCTTCTGGTAGCTTTCTTTTTGATCCCACGAGGCCCAAAATATGGGAAAGTAAAGAAAGTTCTGTCGAATGAGAAGCCGGCCAGAGTGTACACGAAAGCTGATGTCTCGTTGCATAACAAGCGAACGGATTGTTGGATAATTATTAAAGACAAGGTATATGATATTACCTCGTACGTAGAGGAGCACCCGGGTGGTGATGCCATTTTAGATCATGCTGGGGATGATTCGACTGAAGGGTTTTTCGGCCCGCAGCATGCAACCCGAGTCTTTGACATGATCGACGACTTTTACATTGGAGATCTGGTGCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyt-b5

Protein Analysis

116

Amino Acids

12.83

Weight (kDa)

5.72

Isoelectric Point (pI)

26.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyt-b5 PF00173 43 - 113 2.2e-23 Cytochrome b5-like Heme/Steroid binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010898)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G60660
fragaria_vesca FvH4_1g06100 FvH4_1g06100 FvH4_1g06100
malus_domestica MD02G1065500.v1.1
prunus_persica Prupe.7G220000_v2.0.a1 Prupe.7G220000_v2.0.a1
pyrus_communis pycom02g05160
rosa_chinensis RchiOBHm_Chr2g0091851
rosa_laevigata RLG00000016257
rosa_multiflora Rmu_co8418845.1_g000001
rosa_roxburghii Rroxscaffold_2G00149670 Rroxscaffold_2G00149680
rosa_rugosa Rorug02G0021500
rosa_samantha Rh2AG066300 Rh2AG066500 Rh2BG065700 Rh2CG067100 Rh2DG065500
rosa_wichuraiana Rw2G005440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 77
AccII CGCG 1 cut(s) 14
AciI CCGC 4 cut(s) 12, 14, 29, 284
AclWI GGATC 1 cut(s) 52
AcoI YGGCCR 1 cut(s) 114
AcuI CTGAAG 1 cut(s) 288
AfaI GTAC 2 cut(s) 125, 209
AfiI CCNNNNNNNGG 1 cut(s) 77
AjuI GAANNNNNNNTTGG 2 cut(s) 151, 183
AleI CACNNNNGTG 1 cut(s) 225
AluBI AGCT 2 cut(s) 47, 134
AluI AGCT 2 cut(s) 47, 134
Alw21I GWGCWC 1 cut(s) 222
Alw26I GTCTC 1 cut(s) 145
AlwI GGATC 1 cut(s) 52
Ama87I CYCGRG 2 cut(s) 223, 297
AoxI GGCC 3 cut(s) 67, 114, 280
ApeKI GCWGC 1 cut(s) 286
AspLEI GCGC 1 cut(s) 22
AspS9I GGNCC 2 cut(s) 68, 281
AsuC2I CCSGG 2 cut(s) 224, 225
AsuHPI GGTGA 1 cut(s) 242
AvaI CYCGRG 2 cut(s) 223, 297
BauI CACGAG 1 cut(s) 63
Bbv12I GWGCWC 1 cut(s) 222
BbvI GCAGC 1 cut(s) 298
BcnI CCSGG 2 cut(s) 224, 225
BcoDI GTCTC 1 cut(s) 145
BfoI RGCGCY 1 cut(s) 23
BglI GCCNNNNNGGC 1 cut(s) 17
BglII AGATCT 1 cut(s) 334
BisI GCNGC 2 cut(s) 12, 287
BlsI GCNGC 2 cut(s) 13, 288
Bme1390I CCNGG 2 cut(s) 224, 225
BmeT110I CYCGRG 2 cut(s) 223, 297
BmgT120I GGNCC 2 cut(s) 68, 281
BmrFI CCNGG 2 cut(s) 224, 225
BmsI GCATC 1 cut(s) 223
BpuMI CCSGG 2 cut(s) 224, 225
BsaAI YACGTR 1 cut(s) 211
BsaJI CCNNGG 2 cut(s) 12, 223
BsaXI ACNNNNNCTCC 1 cut(s) 324
Bsc4I CCNNNNNNNGG 1 cut(s) 77
Bse118I RCCGGY 1 cut(s) 112
BseDI CCNNGG 2 cut(s) 12, 223
BseGI GGATG 1 cut(s) 262
BseLI CCNNNNNNNGG 1 cut(s) 77
BseRI GAGGAG 1 cut(s) 230
BseXI GCAGC 1 cut(s) 298
BseYI CCCAGC 1 cut(s) 251
Bsh1236I CGCG 1 cut(s) 14
BshFI GGCC 3 cut(s) 69, 116, 282
BsiHKAI GWGCWC 1 cut(s) 222
BsiHKCI CYCGRG 2 cut(s) 223, 297
BsiSI CCGG 2 cut(s) 113, 224
BsiWI CGTACG 1 cut(s) 207
BslI CCNNNNNNNGG 1 cut(s) 77
BsmAI GTCTC 1 cut(s) 145
BsnI GGCC 3 cut(s) 69, 116, 282
BsoBI CYCGRG 2 cut(s) 223, 297
Bsp1286I GDGCHC 1 cut(s) 222
Bsp1407I TGTACA 1 cut(s) 123
Bsp143I GATC 5 cut(s) 6, 57, 244, 312, 334
BspACI CCGC 4 cut(s) 12, 14, 29, 284
BspANI GGCC 3 cut(s) 69, 116, 282
BspFNI CGCG 1 cut(s) 14
BspPI GGATC 1 cut(s) 52
BsrFI RCCGGY 1 cut(s) 112
BsrGI TGTACA 1 cut(s) 123
BssAI RCCGGY 1 cut(s) 112
BssECI CCNNGG 2 cut(s) 12, 223
BssMI GATC 5 cut(s) 6, 57, 244, 312, 334
BssSI CACGAG 1 cut(s) 63
Bst2BI CACGAG 1 cut(s) 63
BstAUI TGTACA 1 cut(s) 123
BstBAI YACGTR 1 cut(s) 211
BstC8I GCNNGC 3 cut(s) 114, 284, 291
BstDSI CCRYGG 1 cut(s) 12
BstF5I GGATG 1 cut(s) 262
BstFNI CGCG 1 cut(s) 14
BstH2I RGCGCY 1 cut(s) 23
BstHHI GCGC 1 cut(s) 22
BstKTI GATC 5 cut(s) 9, 60, 247, 315, 337
BstMAI GTCTC 1 cut(s) 145
BstMBI GATC 5 cut(s) 6, 57, 244, 312, 334
BstMWI GCNNNNNNNGC 3 cut(s) 17, 26, 154
BstNSI RCATGY 1 cut(s) 293
BstSCI CCNGG 2 cut(s) 222, 223
BstSNI TACGTA 1 cut(s) 211
BstUI CGCG 1 cut(s) 14
BstV1I GCAGC 1 cut(s) 298
BstX2I RGATCY 1 cut(s) 334
BstYI RGATCY 1 cut(s) 334
BsuRI GGCC 3 cut(s) 69, 116, 282
BtgI CCRYGG 1 cut(s) 12
BtsCI GGATG 1 cut(s) 262
Cac8I GCNNGC 3 cut(s) 114, 284, 291
CfoI GCGC 1 cut(s) 22
Cfr10I RCCGGY 1 cut(s) 112
Cfr13I GGNCC 2 cut(s) 68, 281
Cfr42I CCGCGG 1 cut(s) 15
Cfr9I CCCGGG 1 cut(s) 223
Csp6I GTAC 2 cut(s) 124, 208
CviAII CATG 3 cut(s) 248, 290, 310
CviJI RGCY 6 cut(s) 47, 69, 112, 116, 134, 282
CviKI_1 RGCY 6 cut(s) 47, 69, 112, 116, 134, 282
CviQI GTAC 2 cut(s) 124, 208
DpnI GATC 5 cut(s) 8, 59, 246, 314, 336
DpnII GATC 5 cut(s) 6, 57, 244, 312, 334
EaeI YGGCCR 1 cut(s) 114
Eco105I TACGTA 1 cut(s) 211
Eco57I CTGAAG 1 cut(s) 288
Eco88I CYCGRG 2 cut(s) 223, 297
FaeI CATG 3 cut(s) 251, 293, 313
FaiI YATR 7 cut(s) 78, 150, 193, 195, 249, 291, 311
FatI CATG 3 cut(s) 247, 289, 309
FauI CCCGC 1 cut(s) 291
Fnu4HI GCNGC 2 cut(s) 12, 287
FokI GGATG 1 cut(s) 269
Fsp4HI GCNGC 2 cut(s) 12, 287
GlaI GCGC 1 cut(s) 21
GluI GCNGC 2 cut(s) 12, 287
GsaI CCCAGC 1 cut(s) 255
HaeII RGCGCY 1 cut(s) 23
HaeIII GGCC 3 cut(s) 69, 116, 282
HapII CCGG 2 cut(s) 113, 224
HhaI GCGC 1 cut(s) 22
Hin1II CATG 3 cut(s) 251, 293, 313
Hin6I GCGC 1 cut(s) 20
HinP1I GCGC 1 cut(s) 20
HinfI GANTC 2 cut(s) 260, 300
HpaII CCGG 2 cut(s) 113, 224
HphI GGTGA 1 cut(s) 242
Hpy166II GTNNAC 2 cut(s) 124, 126
Hpy8I GTNNAC 2 cut(s) 124, 126
Hpy99I CGWCG 1 cut(s) 320
HpyAV CCTTC 2 cut(s) 47, 263
HpyCH4IV ACGT 1 cut(s) 210
HpyCH4V TGCA 2 cut(s) 148, 293
HpyF10VI GCNNNNNNNGC 3 cut(s) 17, 26, 154
HpySE526I ACGT 1 cut(s) 210
Hsp92II CATG 3 cut(s) 251, 293, 313
HspAI GCGC 1 cut(s) 20
KroI GCCGGC 1 cut(s) 112
KroNI GCCGGC 1 cut(s) 114
KspI CCGCGG 1 cut(s) 15
Kzo9I GATC 5 cut(s) 6, 57, 244, 312, 334
LmnI GCTCC 1 cut(s) 217
LpnPI CCDG 7 cut(s) 8, 26, 126, 130, 237, 237, 323
Lsp1109I GCAGC 1 cut(s) 298
LweI GCATC 1 cut(s) 223
MaeII ACGT 1 cut(s) 210
MalI GATC 5 cut(s) 8, 59, 246, 314, 336
MboI GATC 5 cut(s) 6, 57, 244, 312, 334
MflI RGATCY 1 cut(s) 334
MhlI GDGCHC 1 cut(s) 222
MluCI AATT 1 cut(s) 174
MlyI GAGTC 1 cut(s) 309
MmeI TCCRAC 1 cut(s) 149
MnlI CCTC 3 cut(s) 59, 208, 214
MroNI GCCGGC 1 cut(s) 112
MseI TTAA 1 cut(s) 180
MslI CAYNNNNRTG 1 cut(s) 225
MspA1I CMGCKG 1 cut(s) 14
MspI CCGG 2 cut(s) 113, 224
MspR9I CCNGG 2 cut(s) 224, 225
MvnI CGCG 1 cut(s) 14
MwoI GCNNNNNNNGC 3 cut(s) 17, 26, 154
NaeI GCCGGC 1 cut(s) 114
NciI CCSGG 2 cut(s) 224, 225
NdeII GATC 5 cut(s) 6, 57, 244, 312, 334
NgoMIV GCCGGC 1 cut(s) 112
NlaIII CATG 3 cut(s) 251, 293, 313
NspI RCATGY 1 cut(s) 293
OliI CACNNNNGTG 1 cut(s) 225
PaeI GCATGC 1 cut(s) 293
PdiI GCCGGC 1 cut(s) 114
PfeI GAWTC 1 cut(s) 260
Pfl23II CGTACG 1 cut(s) 207
PflMI CCANNNNNTGG 1 cut(s) 77
PkrI GCNGC 2 cut(s) 13, 288
PleI GAGTC 1 cut(s) 308
PpsI GAGTC 1 cut(s) 308
Ppu21I YACGTR 1 cut(s) 211
PspFI CCCAGC 1 cut(s) 251
PspLI CGTACG 1 cut(s) 207
PspPI GGNCC 2 cut(s) 68, 281
PsrI GAACNNNNNNTAC 2 cut(s) 78, 110
PsuI RGATCY 1 cut(s) 334
RsaI GTAC 2 cut(s) 125, 209
RsaNI GTAC 2 cut(s) 124, 208
RseI CAYNNNNRTG 1 cut(s) 225
SacII CCGCGG 1 cut(s) 15
SaqAI TTAA 1 cut(s) 180
SatI GCNGC 2 cut(s) 12, 287
Sau3AI GATC 5 cut(s) 6, 57, 244, 312, 334
Sau96I GGNCC 2 cut(s) 68, 281
SchI GAGTC 1 cut(s) 309
ScrFI CCNGG 2 cut(s) 224, 225
SduI GDGCHC 1 cut(s) 222
SetI ASST 5 cut(s) 49, 136, 192, 206, 213
SfaNI GCATC 1 cut(s) 223
Sfr303I CCGCGG 1 cut(s) 15
SgrBI CCGCGG 1 cut(s) 15
SmaI CCCGGG 1 cut(s) 225
SmiMI CAYNNNNRTG 1 cut(s) 225
SnaBI TACGTA 1 cut(s) 211
SphI GCATGC 1 cut(s) 293
Sse9I AATT 1 cut(s) 174
SsiI CCGC 4 cut(s) 12, 14, 29, 284
StyD4I CCNGG 2 cut(s) 222, 223
TaiI ACGT 1 cut(s) 213
TaqI TCGA 3 cut(s) 101, 263, 315
TasI AATT 1 cut(s) 174
TatI WGTACW 1 cut(s) 123
TauI GCSGC 1 cut(s) 14
TfiI GAWTC 1 cut(s) 260
Tru1I TTAA 1 cut(s) 180
Tru9I TTAA 1 cut(s) 180
TseI GCWGC 1 cut(s) 286
TspGWI ACGGA 1 cut(s) 176
TspMI CCCGGG 1 cut(s) 223
Van91I CCANNNNNTGG 1 cut(s) 77
XceI RCATGY 1 cut(s) 293
XmaI CCCGGG 1 cut(s) 223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.