Rorug02G0021500

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0010898
Physical Location & Seq
Forward (+)
1752066 .. 1754581
2516 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0021500.1

Sequence Viewer

Length: 633 bp
ATGGAATTGTTATCAGAAGAGAAACATTTGCACTCTGTTGATTCTGGTAAACAACTAAAGCCTCTGTCATCTGTACTGGATACTACTGGTACTTCTTTGAAGCTACTACATGCCCACCCTTCTGCTGCACAACATCATCAGAGCCTTGGCCGATCAACATTCTTTAAACGTTCACGTCATTACTATGCCCATCAATACTCTCGGCGTAACTCTGGCAATGTTGCCAATGCATCAACTTCTCGTGGAAAAGGGACTCCTTCACGTGAAGAGAAGCTCTCTTATAAGTTGGCTACTCAATGCAGCTCTGACTCTGGACGTCACTCAGTGAAGGAAAAACCCTTTTTTCGGCCAGCCAGAATAAGGTCTAGTTACTTGGTAATGGATGCTGCCTCAGCTGATCCTATGAAGATGTTATGTGGGATTTGTCAGAAGCCATTGAGGCGCAAACCTTTTTTTCTTGGGATCACGCTGTCTTCTACTGAAGTCTCTGTTGTGGCAGTTTTAGTTTGCGGCCATGTTTACCATGCTGATTGTTTGGAGCAGAAAACAAGCTTTGAAGACAGACGTGACCCTCCCTGTCCTTTGTGTGTTGGGTTGTTGCCCAAGATCGAAGATTCTAGAGGGCAGGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PITH Peptidase_M16 Peptidase_M16_C

Protein Analysis

210

Amino Acids

23.35

Weight (kDa)

9.55

Isoelectric Point (pI)

52.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 139 - 196 3e-06 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010898)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G60660
fragaria_vesca FvH4_1g06100 FvH4_1g06100 FvH4_1g06100
malus_domestica MD02G1065500.v1.1
prunus_persica Prupe.7G220000_v2.0.a1 Prupe.7G220000_v2.0.a1
pyrus_communis pycom02g05160
rosa_chinensis RchiOBHm_Chr2g0091851
rosa_laevigata RLG00000016257
rosa_multiflora Rmu_co8418845.1_g000001
rosa_roxburghii Rroxscaffold_2G00149670 Rroxscaffold_2G00149680
rosa_rugosa Rorug02G0021500
rosa_samantha Rh2AG066300 Rh2AG066500 Rh2BG065700 Rh2CG067100 Rh2DG065500
rosa_wichuraiana Rw2G005440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 282
AatII GACGTC 1 cut(s) 319
Acc36I ACCTGC 1 cut(s) 616
AciI CCGC 1 cut(s) 510
AclI AACGTT 1 cut(s) 169
AclWI GGATC 2 cut(s) 392, 470
AcoI YGGCCR 3 cut(s) 148, 347, 511
AcuI CTGAAG 1 cut(s) 501
AcvI CACGTG 1 cut(s) 263
AcyI GRCGYC 1 cut(s) 316
AdeI CACNNNGTG 1 cut(s) 325
AfaI GTAC 2 cut(s) 75, 91
AfiI CCNNNNNNNGG 2 cut(s) 345, 360
AgsI TTSAA 2 cut(s) 100, 557
AjiI CACGTC 2 cut(s) 176, 566
AluBI AGCT 5 cut(s) 103, 274, 303, 395, 552
AluI AGCT 5 cut(s) 103, 274, 303, 395, 552
Alw26I GTCTC 1 cut(s) 490
AlwI GGATC 2 cut(s) 392, 470
AoxI GGCC 3 cut(s) 148, 347, 511
ApeKI GCWGC 3 cut(s) 125, 300, 386
AspLEI GCGC 1 cut(s) 444
BauI CACGAG 1 cut(s) 240
BbrPI CACGTG 1 cut(s) 263
BbsI GAAGAC 2 cut(s) 465, 564
BbvCI CCTCAGC 1 cut(s) 391
BbvI GCAGC 3 cut(s) 112, 312, 373
BccI CCATC 1 cut(s) 198
BciVI GTATCC 1 cut(s) 73
BcoDI GTCTC 1 cut(s) 490
BfaI CTAG 2 cut(s) 366, 618
BfuAI ACCTGC 1 cut(s) 616
BfuI GTATCC 1 cut(s) 73
BglI GCCNNNNNGGC 1 cut(s) 439
BisI GCNGC 4 cut(s) 126, 301, 387, 511
BlsI GCNGC 4 cut(s) 127, 302, 388, 512
BmgBI CACGTC 2 cut(s) 176, 566
BmsI GCATC 2 cut(s) 239, 373
BpiI GAAGAC 2 cut(s) 465, 564
BplI GAGNNNNNCTC 2 cut(s) 260, 292
Bpu10I CCTNAGC 1 cut(s) 391
BsaAI YACGTR 1 cut(s) 263
BsaHI GRCGYC 1 cut(s) 316
BsaJI CCNNGG 1 cut(s) 145
Bsc4I CCNNNNNNNGG 2 cut(s) 345, 360
Bse1I ACTGG 2 cut(s) 81, 91
Bse3DI GCAATG 1 cut(s) 223
BseDI CCNNGG 1 cut(s) 145
BseGI GGATG 1 cut(s) 388
BseLI CCNNNNNNNGG 2 cut(s) 345, 360
BseMI GCAATG 1 cut(s) 223
BseMII CTCAG 2 cut(s) 336, 405
BseNI ACTGG 2 cut(s) 81, 91
BseXI GCAGC 3 cut(s) 112, 312, 373
BsgI GTGCAG 1 cut(s) 111
BshFI GGCC 3 cut(s) 150, 349, 513
BslFI GGGAC 1 cut(s) 265
BslI CCNNNNNNNGG 2 cut(s) 345, 360
BsmAI GTCTC 1 cut(s) 490
BsmFI GGGAC 1 cut(s) 265
BsnI GGCC 3 cut(s) 150, 349, 513
Bsp143I GATC 4 cut(s) 152, 397, 462, 606
BspACI CCGC 1 cut(s) 510
BspANI GGCC 3 cut(s) 150, 349, 513
BspCNI CTCAG 2 cut(s) 335, 404
BspMI ACCTGC 1 cut(s) 616
BspPI GGATC 2 cut(s) 392, 470
BsrDI GCAATG 1 cut(s) 223
BsrI ACTGG 2 cut(s) 81, 91
BssECI CCNNGG 1 cut(s) 145
BssMI GATC 4 cut(s) 152, 397, 462, 606
BssNI GRCGYC 1 cut(s) 316
BssSI CACGAG 1 cut(s) 240
BssT1I CCWWGG 1 cut(s) 145
Bst2BI CACGAG 1 cut(s) 240
Bst6I CTCTTC 2 cut(s) 12, 261
BstACI GRCGYC 1 cut(s) 316
BstBAI YACGTR 1 cut(s) 263
BstC8I GCNNGC 1 cut(s) 351
BstDEI CTNAG 2 cut(s) 322, 391
BstF5I GGATG 1 cut(s) 388
BstHHI GCGC 1 cut(s) 444
BstKTI GATC 4 cut(s) 155, 400, 465, 609
BstMAI GTCTC 1 cut(s) 490
BstMBI GATC 4 cut(s) 152, 397, 462, 606
BstMWI GCNNNNNNNGC 2 cut(s) 392, 439
BstNSI RCATGY 1 cut(s) 113
BstV1I GCAGC 3 cut(s) 112, 312, 373
BstV2I GAAGAC 2 cut(s) 465, 564
BsuI GTATCC 1 cut(s) 73
BsuRI GGCC 3 cut(s) 150, 349, 513
BtrI CACGTC 2 cut(s) 176, 566
BtsCI GGATG 1 cut(s) 388
BtsIMutI CAGTG 1 cut(s) 330
BveI ACCTGC 1 cut(s) 616
Cac8I GCNNGC 1 cut(s) 351
CfoI GCGC 1 cut(s) 444
Csp6I GTAC 2 cut(s) 74, 90
CviAII CATG 3 cut(s) 110, 515, 524
CviQI GTAC 2 cut(s) 74, 90
DdeI CTNAG 2 cut(s) 322, 391
DpnI GATC 4 cut(s) 154, 399, 464, 608
DpnII GATC 4 cut(s) 152, 397, 462, 606
DraI TTTAAA 1 cut(s) 166
DraIII CACNNNGTG 1 cut(s) 325
EaeI YGGCCR 3 cut(s) 148, 347, 511
Eam1104I CTCTTC 2 cut(s) 12, 261
EarI CTCTTC 2 cut(s) 12, 261
Eco130I CCWWGG 1 cut(s) 145
Eco57I CTGAAG 1 cut(s) 501
Eco72I CACGTG 1 cut(s) 263
EcoT14I CCWWGG 1 cut(s) 145
EcoT22I ATGCAT 1 cut(s) 232
ErhI CCWWGG 1 cut(s) 145
FaeI CATG 3 cut(s) 113, 518, 527
FaiI YATR 7 cut(s) 111, 186, 282, 404, 415, 516, 525
FaqI GGGAC 1 cut(s) 265
FatI CATG 3 cut(s) 109, 514, 523
Fnu4HI GCNGC 4 cut(s) 126, 301, 387, 511
FokI GGATG 1 cut(s) 395
Fsp4HI GCNGC 4 cut(s) 126, 301, 387, 511
FspBI CTAG 2 cut(s) 366, 618
GlaI GCGC 1 cut(s) 443
GluI GCNGC 4 cut(s) 126, 301, 387, 511
HaeIII GGCC 3 cut(s) 150, 349, 513
HhaI GCGC 1 cut(s) 444
Hin1I GRCGYC 1 cut(s) 316
Hin1II CATG 3 cut(s) 113, 518, 527
Hin6I GCGC 1 cut(s) 442
HinP1I GCGC 1 cut(s) 442
HindIII AAGCTT 1 cut(s) 550
HinfI GANTC 4 cut(s) 41, 253, 308, 614
Hpy166II GTNNAC 3 cut(s) 50, 173, 520
Hpy188I TCNGA 4 cut(s) 16, 141, 307, 429
Hpy188III TCNNGA 2 cut(s) 312, 618
Hpy8I GTNNAC 3 cut(s) 50, 173, 520
HpyAV CCTTC 3 cut(s) 129, 267, 322
HpyCH4IV ACGT 5 cut(s) 169, 175, 262, 316, 565
HpyCH4V TGCA 4 cut(s) 31, 128, 230, 300
HpyF10VI GCNNNNNNNGC 2 cut(s) 392, 439
HpyF3I CTNAG 2 cut(s) 322, 391
HpySE526I ACGT 5 cut(s) 169, 175, 262, 316, 565
Hsp92I GRCGYC 1 cut(s) 316
Hsp92II CATG 3 cut(s) 113, 518, 527
HspAI GCGC 1 cut(s) 442
Kzo9I GATC 4 cut(s) 152, 397, 462, 606
LmnI GCTCC 1 cut(s) 538
LpnPI CCDG 9 cut(s) 30, 62, 72, 198, 297, 363, 367, 589, 611
Lsp1109I GCAGC 3 cut(s) 112, 312, 373
LweI GCATC 2 cut(s) 239, 373
MaeI CTAG 2 cut(s) 366, 618
MaeII ACGT 5 cut(s) 169, 175, 262, 316, 565
MaeIII GTNAC 4 cut(s) 206, 317, 368, 566
MalI GATC 4 cut(s) 154, 399, 464, 608
MboI GATC 4 cut(s) 152, 397, 462, 606
MboII GAAGA 6 cut(s) 29, 278, 418, 465, 569, 623
MluCI AATT 1 cut(s) 5
MlyI GAGTC 2 cut(s) 247, 302
MnlI CCTC 5 cut(s) 72, 400, 432, 582, 614
Mph1103I ATGCAT 1 cut(s) 232
MseI TTAA 1 cut(s) 165
MslI CAYNNNNRTG 1 cut(s) 183
MspA1I CMGCKG 1 cut(s) 395
MwoI GCNNNNNNNGC 2 cut(s) 392, 439
NdeII GATC 4 cut(s) 152, 397, 462, 606
NlaIII CATG 3 cut(s) 113, 518, 527
NmeAIII GCCGAG 1 cut(s) 181
NmuCI GTSAC 2 cut(s) 317, 566
NsiI ATGCAT 1 cut(s) 232
NspI RCATGY 1 cut(s) 113
PfeI GAWTC 2 cut(s) 41, 614
PkrI GCNGC 4 cut(s) 127, 302, 388, 512
PleI GAGTC 2 cut(s) 247, 302
PmaCI CACGTG 1 cut(s) 263
PmlI CACGTG 1 cut(s) 263
PpsI GAGTC 2 cut(s) 247, 302
Ppu21I YACGTR 1 cut(s) 263
PsiI TTATAA 1 cut(s) 282
Psp1406I AACGTT 1 cut(s) 169
PspCI CACGTG 1 cut(s) 263
PvuII CAGCTG 1 cut(s) 395
RsaI GTAC 2 cut(s) 75, 91
RsaNI GTAC 2 cut(s) 74, 90
RseI CAYNNNNRTG 1 cut(s) 183
SaqAI TTAA 1 cut(s) 165
SatI GCNGC 4 cut(s) 126, 301, 387, 511
Sau3AI GATC 4 cut(s) 152, 397, 462, 606
SchI GAGTC 2 cut(s) 247, 302
SfaNI GCATC 2 cut(s) 239, 373
SmiMI CAYNNNNRTG 1 cut(s) 183
Sse9I AATT 1 cut(s) 5
SsiI CCGC 1 cut(s) 510
SspMI CTAG 2 cut(s) 366, 618
StyI CCWWGG 1 cut(s) 145
TaiI ACGT 5 cut(s) 172, 178, 265, 319, 568
TaqI TCGA 1 cut(s) 609
TasI AATT 1 cut(s) 5
TatI WGTACW 1 cut(s) 73
TauI GCSGC 1 cut(s) 513
TfiI GAWTC 2 cut(s) 41, 614
Tru1I TTAA 1 cut(s) 165
Tru9I TTAA 1 cut(s) 165
TscAI CASTG 1 cut(s) 330
TseFI GTSAC 2 cut(s) 317, 566
TseI GCWGC 3 cut(s) 125, 300, 386
Tsp45I GTSAC 2 cut(s) 317, 566
TspDTI ATGAA 1 cut(s) 419
TspRI CASTG 1 cut(s) 330
XbaI TCTAGA 1 cut(s) 617
XceI RCATGY 1 cut(s) 113
XspI CTAG 2 cut(s) 366, 618
ZraI GACGTC 1 cut(s) 317
Zsp2I ATGCAT 1 cut(s) 232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.