Rh2AG066300

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0010898
Physical Location & Seq
Forward (+)
5549652 .. 5550251
600 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG066300.1

Sequence Viewer

Length: 600 bp
ATGGCTCTCCTGGTTTCTATTTTATTTCTCCTTTTAATAGTCCTCGGCGTCGTAAGAATTAAACATGAACTTCCCCCCGCCCTAGCTATAGTCATCTGTATACTCATGATACTAGATGTCTTGATAATATACACCATTGTTCTTCTTTCCATTAATCCCTTTGATCATGATCATTCCATTGAAACTAATTCCATCATAACAGTCCTCCGCATTGCGTTTGGGTTCCCTGCGTTACTTCTGGTATATGACTTAATACTCATGGTCAACCTGTACTGTTCAGGGAATAAGATGATCACAGTGATGCAACATCTACAGAATAATGCGAGTTTTTTTCTGGTGAGAATCGGTGCCAAGAGGCCTAAGGGCTATGTGATTTTCAGTTATCATATGGATGAAGAAACCGGGTTTTGCAGCCATGAGTGTTCTATTTGCTTGATGGACTACAAGGAAAAAGACGTGTGCGCATTGCTAGATAAATGTGGACACACATTTCACCATATATGCCTCCATATGTGTTTGAATGAATCTACCCGTTGTCCGCTTTGTCGTCAAAATATCAAAGATTGTTCACAAAGGGAACTCTATACACTCTATATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

199

Amino Acids

22.75

Weight (kDa)

6.53

Isoelectric Point (pI)

43.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 140 - 183 2.7e-09 Ring finger domain
zf-RING_11 PF17123 140 - 169 7.5e-07 RING-like zinc finger
zf-RING_UBOX PF13445 141 - 180 3.3e-06 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010898)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G60660
fragaria_vesca FvH4_1g06100 FvH4_1g06100 FvH4_1g06100
malus_domestica MD02G1065500.v1.1
prunus_persica Prupe.7G220000_v2.0.a1 Prupe.7G220000_v2.0.a1
pyrus_communis pycom02g05160
rosa_chinensis RchiOBHm_Chr2g0091851
rosa_laevigata RLG00000016257
rosa_multiflora Rmu_co8418845.1_g000001
rosa_roxburghii Rroxscaffold_2G00149670 Rroxscaffold_2G00149680
rosa_rugosa Rorug02G0021500
rosa_samantha Rh2AG066300 Rh2AG066500 Rh2BG065700 Rh2CG067100 Rh2DG065500
rosa_wichuraiana Rw2G005440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 463
AccB1I GGYRCC 1 cut(s) 347
AccI GTMKAC 1 cut(s) 100
AciI CCGC 3 cut(s) 78, 208, 539
AcyI GRCGYC 1 cut(s) 48
AfaI GTAC 1 cut(s) 272
AflIII ACRYGT 1 cut(s) 456
AgsI TTSAA 2 cut(s) 182, 520
AjiI CACGTC 1 cut(s) 457
AjnI CCWGG 1 cut(s) 9
AluBI AGCT 1 cut(s) 86
AluI AGCT 1 cut(s) 86
AoxI GGCC 1 cut(s) 356
ApeKI GCWGC 1 cut(s) 411
AseI ATTAAT 1 cut(s) 153
AspLEI GCGC 1 cut(s) 464
AsuC2I CCSGG 1 cut(s) 403
AsuHPI GGTGA 2 cut(s) 349, 485
AxyI CCTNAGG 1 cut(s) 360
BanI GGYRCC 1 cut(s) 347
BbvI GCAGC 1 cut(s) 423
BccI CCATC 2 cut(s) 200, 430
BciT130I CCWGG 1 cut(s) 11
BclI TGATCA 3 cut(s) 163, 169, 291
BcnI CCSGG 1 cut(s) 403
BfaI CTAG 3 cut(s) 83, 113, 470
BfmI CTRYAG 2 cut(s) 87, 311
BisI GCNGC 1 cut(s) 412
BlsI GCNGC 1 cut(s) 413
Bme1390I CCNGG 2 cut(s) 11, 403
BmgBI CACGTC 1 cut(s) 457
BmiI GGNNCC 2 cut(s) 224, 349
BmrFI CCNGG 2 cut(s) 11, 403
BmsI GCATC 1 cut(s) 291
BpuMI CCSGG 1 cut(s) 403
BsaBI GATNNNNATC 1 cut(s) 168
BsaHI GRCGYC 1 cut(s) 48
BsaJI CCNNGG 1 cut(s) 43
Bse21I CCTNAGG 1 cut(s) 360
Bse3DI GCAATG 2 cut(s) 210, 464
Bse8I GATNNNNATC 1 cut(s) 168
BseBI CCWGG 1 cut(s) 11
BseDI CCNNGG 1 cut(s) 43
BseGI GGATG 1 cut(s) 397
BseJI GATNNNNATC 1 cut(s) 168
BseMI GCAATG 2 cut(s) 210, 464
BseXI GCAGC 1 cut(s) 423
BshFI GGCC 1 cut(s) 358
BshNI GGYRCC 1 cut(s) 347
BsiSI CCGG 1 cut(s) 402
BsnI GGCC 1 cut(s) 358
Bsp143I GATC 3 cut(s) 163, 169, 291
BspACI CCGC 3 cut(s) 78, 208, 539
BspANI GGCC 1 cut(s) 358
BspHI TCATGA 2 cut(s) 105, 166
BspLI GGNNCC 2 cut(s) 224, 349
BspT107I GGYRCC 1 cut(s) 347
BsrDI GCAATG 2 cut(s) 210, 464
BssECI CCNNGG 1 cut(s) 43
BssMI GATC 3 cut(s) 163, 169, 291
BssNAI GTATAC 1 cut(s) 101
BssNI GRCGYC 1 cut(s) 48
Bst1107I GTATAC 1 cut(s) 101
Bst2UI CCWGG 1 cut(s) 11
Bst4CI ACNGT 3 cut(s) 202, 275, 298
BstACI GRCGYC 1 cut(s) 48
BstDEI CTNAG 1 cut(s) 360
BstF5I GGATG 1 cut(s) 397
BstHHI GCGC 1 cut(s) 464
BstKTI GATC 3 cut(s) 166, 172, 294
BstMBI GATC 3 cut(s) 163, 169, 291
BstNI CCWGG 1 cut(s) 11
BstSCI CCNGG 2 cut(s) 9, 401
BstSFI CTRYAG 2 cut(s) 87, 311
BstV1I GCAGC 1 cut(s) 423
BstZ17I GTATAC 1 cut(s) 101
Bsu36I CCTNAGG 1 cut(s) 360
BsuRI GGCC 1 cut(s) 358
BtrI CACGTC 1 cut(s) 457
BtsCI GGATG 1 cut(s) 397
BtsIMutI CAGTG 1 cut(s) 303
CciI TCATGA 2 cut(s) 105, 166
CfoI GCGC 1 cut(s) 464
CseI GACGC 1 cut(s) 37
Csp6I GTAC 1 cut(s) 271
CviAII CATG 5 cut(s) 65, 106, 167, 259, 416
CviJI RGCY 5 cut(s) 5, 86, 358, 366, 414
CviKI_1 RGCY 5 cut(s) 5, 86, 358, 366, 414
CviQI GTAC 1 cut(s) 271
DdeI CTNAG 1 cut(s) 360
DpnI GATC 3 cut(s) 165, 171, 293
DpnII GATC 3 cut(s) 163, 169, 291
Eco147I AGGCCT 1 cut(s) 358
Eco81I CCTNAGG 1 cut(s) 360
EcoRII CCWGG 1 cut(s) 9
FaeI CATG 5 cut(s) 68, 109, 170, 262, 419
FatI CATG 5 cut(s) 64, 105, 166, 258, 415
FauI CCCGC 1 cut(s) 85
FauNDI CATATG 2 cut(s) 387, 510
FbaI TGATCA 3 cut(s) 163, 169, 291
FblI GTMKAC 1 cut(s) 100
Fnu4HI GCNGC 1 cut(s) 412
FokI GGATG 1 cut(s) 404
Fsp4HI GCNGC 1 cut(s) 412
FspAI RTGCGCAY 1 cut(s) 463
FspBI CTAG 3 cut(s) 83, 113, 470
FspI TGCGCA 1 cut(s) 463
GlaI GCGC 1 cut(s) 463
GluI GCNGC 1 cut(s) 412
HaeIII GGCC 1 cut(s) 358
HapII CCGG 1 cut(s) 402
HgaI GACGC 1 cut(s) 37
HhaI GCGC 1 cut(s) 464
Hin1I GRCGYC 1 cut(s) 48
Hin1II CATG 5 cut(s) 68, 109, 170, 262, 419
Hin6I GCGC 1 cut(s) 462
HinP1I GCGC 1 cut(s) 462
HincII GTYRAC 1 cut(s) 265
HindII GTYRAC 1 cut(s) 265
HinfI GANTC 2 cut(s) 342, 524
HpaII CCGG 1 cut(s) 402
HphI GGTGA 2 cut(s) 349, 485
Hpy166II GTNNAC 4 cut(s) 101, 265, 482, 569
Hpy188III TCNNGA 3 cut(s) 106, 121, 167
Hpy8I GTNNAC 4 cut(s) 101, 265, 482, 569
Hpy99I CGWCG 1 cut(s) 53
HpyCH4III ACNGT 3 cut(s) 202, 275, 298
HpyCH4IV ACGT 1 cut(s) 456
HpyCH4V TGCA 2 cut(s) 304, 411
HpyF3I CTNAG 1 cut(s) 360
HpySE526I ACGT 1 cut(s) 456
Hsp92I GRCGYC 1 cut(s) 48
Hsp92II CATG 5 cut(s) 68, 109, 170, 262, 419
HspAI GCGC 1 cut(s) 462
Ksp22I TGATCA 3 cut(s) 163, 169, 291
Kzo9I GATC 3 cut(s) 163, 169, 291
LpnPI CCDG 7 cut(s) 23, 224, 240, 264, 281, 320, 415
Lsp1109I GCAGC 1 cut(s) 423
LweI GCATC 1 cut(s) 291
MaeI CTAG 3 cut(s) 83, 113, 470
MaeII ACGT 1 cut(s) 456
MaeIII GTNAC 1 cut(s) 231
MalI GATC 3 cut(s) 165, 171, 293
MboI GATC 3 cut(s) 163, 169, 291
MboII GAAGA 2 cut(s) 134, 407
MluCI AATT 2 cut(s) 57, 187
MnlI CCTC 4 cut(s) 53, 215, 348, 515
MseI TTAA 4 cut(s) 35, 60, 153, 251
MslI CAYNNNNRTG 2 cut(s) 299, 390
MspI CCGG 1 cut(s) 402
MspR9I CCNGG 2 cut(s) 11, 403
MvaI CCWGG 1 cut(s) 11
NciI CCSGG 1 cut(s) 403
NdeI CATATG 2 cut(s) 387, 510
NdeII GATC 3 cut(s) 163, 169, 291
NlaIII CATG 5 cut(s) 68, 109, 170, 262, 419
NlaIV GGNNCC 2 cut(s) 224, 349
NmeAIII GCCGAG 1 cut(s) 24
NsbI TGCGCA 1 cut(s) 463
PagI TCATGA 2 cut(s) 105, 166
PceI AGGCCT 1 cut(s) 358
PfeI GAWTC 2 cut(s) 342, 524
PkrI GCNGC 1 cut(s) 413
PshBI ATTAAT 1 cut(s) 153
Psp6I CCWGG 1 cut(s) 9
PspGI CCWGG 1 cut(s) 9
PspN4I GGNNCC 2 cut(s) 224, 349
RsaI GTAC 1 cut(s) 272
RsaNI GTAC 1 cut(s) 271
RseI CAYNNNNRTG 2 cut(s) 299, 390
SaqAI TTAA 4 cut(s) 35, 60, 153, 251
SatI GCNGC 1 cut(s) 412
Sau3AI GATC 3 cut(s) 163, 169, 291
ScrFI CCNGG 2 cut(s) 11, 403
SetI ASST 3 cut(s) 88, 270, 459
SfaNI GCATC 1 cut(s) 291
SfcI CTRYAG 2 cut(s) 87, 311
SmiMI CAYNNNNRTG 2 cut(s) 299, 390
Sse9I AATT 2 cut(s) 57, 187
SseBI AGGCCT 1 cut(s) 358
SsiI CCGC 3 cut(s) 78, 208, 539
SspMI CTAG 3 cut(s) 83, 113, 470
StuI AGGCCT 1 cut(s) 358
StyD4I CCNGG 2 cut(s) 9, 401
TaaI ACNGT 3 cut(s) 202, 275, 298
TaiI ACGT 1 cut(s) 459
TasI AATT 2 cut(s) 57, 187
TatI WGTACW 1 cut(s) 270
TfiI GAWTC 2 cut(s) 342, 524
Tru1I TTAA 4 cut(s) 35, 60, 153, 251
Tru9I TTAA 4 cut(s) 35, 60, 153, 251
TscAI CASTG 1 cut(s) 303
TseI GCWGC 1 cut(s) 411
TspDTI ATGAA 3 cut(s) 81, 408, 537
TspRI CASTG 1 cut(s) 303
VspI ATTAAT 1 cut(s) 153
XmiI GTMKAC 1 cut(s) 100
XspI CTAG 3 cut(s) 83, 113, 470
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.