AT1G62250

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0009340
Physical Location & Seq
Reverse (-)
22994538 .. 22997709
3172 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G62250.2

Sequence Viewer

Length: 804 bp
ATGTTAGTTGCGTATCCTGCGGGAACTATCAACAAATTCCAAATCCCTATTAGCTGCTATGCCGCTTTGCGCAGATTCAAGACCTCGCCTTTTGGTCCTCTGATTAGTTGTTCTGTCTCCCCAAGATCAATAGTTCCTTGTAGCAATGTAGAGTGTTATCATTCATCTTTTTCTACAAAATATCAAATTCAGCCTCGAAGGAGATGGATGTTTTGTAACTCACGCAACAACTCGATTTCATCGGATGATGAGTACCGTTCATCACCCAACATAGCTATAAGTCTTTTAAGACGTTACAGGACCGTGATTGGACGCGGTGAAGGCGAAACCTTAAAAGAATTCATATCCGCTGGTGTGAATGCATATGCGCTCGGCTGCACAGATGAAGATTTGAGAAAAGAACTAATGGCTATGAAAGATTCGGGTTTAGAGATTGAGAGAATGGAGAATTACGGCGGAAGCACACAGACTAAATCCAAAATTACGCTGTCTGAGGTAGATGAATGCATATTGTGGCTTAGGATTGTATTCATCACGATCTTGTGTACGCCGCAACCAACAGTCATAAGATGGTCATCGACTCCTTCGGTTTCTGATGAAATTCTAAGCAAATGGAGAGGCTTTTGCGCGGTTATAGCAAATGCATACTATATTAGAGGAATGGCTTGGCTGCCTGTGAAGACTCTTCAGTTGGAGCAGATGGCGGTGACAGGACAATCAGAGGAGCCGTCCGTTGTGGCCAGCCGAATGAGACTGGTGTTTAGCACTCTCGAGGTAGTGAGTCCTCAATGGCCACGGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

267

Amino Acids

30.14

Weight (kDa)

8.96

Isoelectric Point (pI)

71.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7876 PF25286 77 - 223 9.8e-73 Domain of unknown function (DUF7876)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 71
AccII CGCG 2 cut(s) 315, 629
AciI CCGC 8 cut(s) 20, 63, 315, 348, 456, 551, 629, 704
AcoI YGGCCR 2 cut(s) 738, 791
AcsI RAATTY 4 cut(s) 35, 186, 338, 600
AcuI CTGAAG 1 cut(s) 671
AfaI GTAC 2 cut(s) 254, 547
AgsI TTSAA 1 cut(s) 79
AluBI AGCT 2 cut(s) 54, 275
AluI AGCT 2 cut(s) 54, 275
Alw26I GTCTC 2 cut(s) 121, 745
Ama87I CYCGRG 1 cut(s) 770
AoxI GGCC 2 cut(s) 738, 791
ApeKI GCWGC 3 cut(s) 54, 375, 670
ApoI RAATTY 4 cut(s) 35, 186, 338, 600
AspLEI GCGC 3 cut(s) 72, 370, 629
AspS9I GGNCC 2 cut(s) 95, 300
AsuHPI GGTGA 3 cut(s) 255, 329, 718
AvaI CYCGRG 1 cut(s) 770
AvaII GGWCC 2 cut(s) 95, 300
BalI TGGCCA 2 cut(s) 740, 793
BbsI GAAGAC 1 cut(s) 686
BbvI GCAGC 3 cut(s) 41, 362, 657
BccI CCATC 3 cut(s) 198, 564, 694
BceAI ACGGC 2 cut(s) 469, 712
BciVI GTATCC 1 cut(s) 24
BcoDI GTCTC 2 cut(s) 121, 745
BfuI GTATCC 1 cut(s) 24
BisI GCNGC 5 cut(s) 55, 63, 376, 551, 671
BlsI GCNGC 5 cut(s) 56, 64, 377, 552, 672
Bme18I GGWCC 2 cut(s) 95, 300
BmeT110I CYCGRG 1 cut(s) 770
BmgT120I GGNCC 2 cut(s) 95, 300
BmiI GGNNCC 1 cut(s) 726
BpiI GAAGAC 1 cut(s) 686
Bpu10I CCTNAGC 1 cut(s) 518
BsaBI GATNNNNATC 1 cut(s) 574
BsaJI CCNNGG 1 cut(s) 794
Bse1I ACTGG 1 cut(s) 759
Bse3DI GCAATG 1 cut(s) 151
Bse8I GATNNNNATC 1 cut(s) 574
BseDI CCNNGG 1 cut(s) 794
BseGI GGATG 2 cut(s) 213, 250
BseJI GATNNNNATC 1 cut(s) 574
BseMI GCAATG 1 cut(s) 151
BseMII CTCAG 1 cut(s) 483
BseNI ACTGG 1 cut(s) 759
BseRI GAGGAG 1 cut(s) 737
BseXI GCAGC 3 cut(s) 41, 362, 657
BsgI GTGCAG 1 cut(s) 361
Bsh1236I CGCG 2 cut(s) 315, 629
BshFI GGCC 2 cut(s) 740, 793
BsiHKCI CYCGRG 1 cut(s) 770
BsmAI GTCTC 2 cut(s) 121, 745
BsmI GAATGC 2 cut(s) 364, 509
BsnI GGCC 2 cut(s) 740, 793
BsoBI CYCGRG 1 cut(s) 770
Bsp143I GATC 2 cut(s) 125, 537
BspACI CCGC 8 cut(s) 20, 63, 315, 348, 456, 551, 629, 704
BspANI GGCC 2 cut(s) 740, 793
BspCNI CTCAG 1 cut(s) 484
BspFNI CGCG 2 cut(s) 315, 629
BspLI GGNNCC 1 cut(s) 726
BsrDI GCAATG 1 cut(s) 151
BsrI ACTGG 1 cut(s) 759
BssECI CCNNGG 1 cut(s) 794
BssMI GATC 2 cut(s) 125, 537
Bst4CI ACNGT 3 cut(s) 257, 304, 562
Bst6I CTCTTC 1 cut(s) 690
BstC8I GCNNGC 1 cut(s) 742
BstDEI CTNAG 3 cut(s) 492, 518, 605
BstDSI CCRYGG 1 cut(s) 794
BstF5I GGATG 2 cut(s) 213, 250
BstFNI CGCG 2 cut(s) 315, 629
BstHHI GCGC 3 cut(s) 72, 370, 629
BstKTI GATC 2 cut(s) 128, 540
BstMAI GTCTC 2 cut(s) 121, 745
BstMBI GATC 2 cut(s) 125, 537
BstMWI GCNNNNNNNGC 3 cut(s) 17, 321, 635
BstUI CGCG 2 cut(s) 315, 629
BstV1I GCAGC 3 cut(s) 41, 362, 657
BstV2I GAAGAC 1 cut(s) 686
BsuI GTATCC 1 cut(s) 24
BsuRI GGCC 2 cut(s) 740, 793
BtgI CCRYGG 1 cut(s) 794
BtsCI GGATG 2 cut(s) 213, 250
Cac8I GCNNGC 1 cut(s) 742
CfoI GCGC 3 cut(s) 72, 370, 629
Cfr13I GGNCC 2 cut(s) 95, 300
CseI GACGC 1 cut(s) 321
Csp6I GTAC 2 cut(s) 253, 546
CviQI GTAC 2 cut(s) 253, 546
DdeI CTNAG 3 cut(s) 492, 518, 605
DpnI GATC 2 cut(s) 127, 539
DpnII GATC 2 cut(s) 125, 537
EaeI YGGCCR 2 cut(s) 738, 791
Eam1104I CTCTTC 1 cut(s) 690
EarI CTCTTC 1 cut(s) 690
EciI GGCGGA 1 cut(s) 471
Eco47I GGWCC 2 cut(s) 95, 300
Eco57I CTGAAG 1 cut(s) 671
Eco88I CYCGRG 1 cut(s) 770
EcoRI GAATTC 1 cut(s) 338
EcoT22I ATGCAT 3 cut(s) 364, 509, 646
FauI CCCGC 1 cut(s) 13
FauNDI CATATG 1 cut(s) 364
Fnu4HI GCNGC 5 cut(s) 55, 63, 376, 551, 671
FokI GGATG 2 cut(s) 220, 257
Fsp4HI GCNGC 5 cut(s) 55, 63, 376, 551, 671
FspI TGCGCA 1 cut(s) 71
GlaI GCGC 3 cut(s) 71, 369, 628
GluI GCNGC 5 cut(s) 55, 63, 376, 551, 671
HaeIII GGCC 2 cut(s) 740, 793
HgaI GACGC 1 cut(s) 321
HhaI GCGC 3 cut(s) 72, 370, 629
Hin6I GCGC 3 cut(s) 70, 368, 627
HinP1I GCGC 3 cut(s) 70, 368, 627
HinfI GANTC 5 cut(s) 75, 419, 580, 682, 781
HphI GGTGA 3 cut(s) 255, 329, 718
Hpy166II GTNNAC 1 cut(s) 546
Hpy188I TCNGA 5 cut(s) 102, 244, 493, 595, 721
Hpy188III TCNNGA 3 cut(s) 79, 535, 770
Hpy8I GTNNAC 1 cut(s) 546
HpyAV CCTTC 3 cut(s) 192, 314, 594
HpyCH4III ACNGT 3 cut(s) 257, 304, 562
HpyCH4IV ACGT 1 cut(s) 292
HpyCH4V TGCA 4 cut(s) 362, 378, 507, 644
HpyF10VI GCNNNNNNNGC 3 cut(s) 17, 321, 635
HpyF3I CTNAG 3 cut(s) 492, 518, 605
HpySE526I ACGT 1 cut(s) 292
HspAI GCGC 3 cut(s) 70, 368, 627
Kzo9I GATC 2 cut(s) 125, 537
LmnI GCTCC 2 cut(s) 694, 724
LpnPI CCDG 7 cut(s) 30, 283, 336, 687, 696, 740, 754
Lsp1109I GCAGC 3 cut(s) 41, 362, 657
MaeII ACGT 1 cut(s) 292
MaeIII GTNAC 3 cut(s) 215, 293, 706
MalI GATC 2 cut(s) 127, 539
MboI GATC 2 cut(s) 125, 537
MboII GAAGA 3 cut(s) 398, 677, 691
MlsI TGGCCA 2 cut(s) 740, 793
MluCI AATT 6 cut(s) 35, 186, 338, 448, 480, 600
MluNI TGGCCA 2 cut(s) 740, 793
MlyI GAGTC 3 cut(s) 574, 676, 790
MmeI TCCRAC 1 cut(s) 672
MnlI CCTC 9 cut(s) 94, 108, 204, 487, 611, 650, 715, 766, 795
Mox20I TGGCCA 2 cut(s) 740, 793
Mph1103I ATGCAT 3 cut(s) 364, 509, 646
MscI TGGCCA 2 cut(s) 740, 793
MseI TTAA 3 cut(s) 287, 332, 802
Msp20I TGGCCA 2 cut(s) 740, 793
MspA1I CMGCKG 1 cut(s) 350
Mva1269I GAATGC 2 cut(s) 364, 509
MvnI CGCG 2 cut(s) 315, 629
MwoI GCNNNNNNNGC 3 cut(s) 17, 321, 635
NdeI CATATG 1 cut(s) 364
NdeII GATC 2 cut(s) 125, 537
NlaIV GGNNCC 1 cut(s) 726
NmeAIII GCCGAG 1 cut(s) 351
NmuCI GTSAC 1 cut(s) 706
NsbI TGCGCA 1 cut(s) 71
NsiI ATGCAT 3 cut(s) 364, 509, 646
PaeR7I CTCGAG 1 cut(s) 770
PctI GAATGC 2 cut(s) 364, 509
PfeI GAWTC 2 cut(s) 75, 419
PkrI GCNGC 5 cut(s) 56, 64, 377, 552, 672
PleI GAGTC 3 cut(s) 574, 676, 789
PpsI GAGTC 3 cut(s) 574, 676, 789
PspN4I GGNNCC 1 cut(s) 726
PspPI GGNCC 2 cut(s) 95, 300
RsaI GTAC 2 cut(s) 254, 547
RsaNI GTAC 2 cut(s) 253, 546
SaqAI TTAA 3 cut(s) 287, 332, 802
SatI GCNGC 5 cut(s) 55, 63, 376, 551, 671
Sau3AI GATC 2 cut(s) 125, 537
Sau96I GGNCC 2 cut(s) 95, 300
SchI GAGTC 3 cut(s) 574, 676, 790
SetI ASST 7 cut(s) 56, 86, 277, 295, 332, 498, 777
Sfr274I CTCGAG 1 cut(s) 770
SinI GGWCC 2 cut(s) 95, 300
SlaI CTCGAG 1 cut(s) 770
SmlI CTYRAG 1 cut(s) 770
SmoI CTYRAG 1 cut(s) 770
Sse9I AATT 6 cut(s) 35, 186, 338, 448, 480, 600
SsiI CCGC 8 cut(s) 20, 63, 315, 348, 456, 551, 629, 704
TaaI ACNGT 3 cut(s) 257, 304, 562
TaiI ACGT 1 cut(s) 295
TaqI TCGA 4 cut(s) 196, 233, 578, 771
TasI AATT 6 cut(s) 35, 186, 338, 448, 480, 600
TauI GCSGC 2 cut(s) 65, 553
TfiI GAWTC 2 cut(s) 75, 419
Tru1I TTAA 3 cut(s) 287, 332, 802
Tru9I TTAA 3 cut(s) 287, 332, 802
TseFI GTSAC 1 cut(s) 706
TseI GCWGC 3 cut(s) 54, 375, 670
Tsp45I GTSAC 1 cut(s) 706
TspDTI ATGAA 9 cut(s) 153, 228, 249, 331, 399, 428, 516, 520, 612
TspGWI ACGGA 1 cut(s) 721
VpaK11BI GGWCC 2 cut(s) 95, 300
XapI RAATTY 4 cut(s) 35, 186, 338, 600
XhoI CTCGAG 1 cut(s) 770
Zsp2I ATGCAT 3 cut(s) 364, 509, 646
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.