Rh5BG530900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0009340
Physical Location & Seq
Reverse (-)
83579750 .. 83581974
2225 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG530900.1

Sequence Viewer

Length: 801 bp
ATGTTGATGGTTTATGCTCCAAGAGCTGTTACATGTCTAGATTCTCATATACTGAATGAGACATTATTACCCTGTAAGAATACACATGTGAATTCCTTAAACAATGTTTCAATCGGTCTTGGCCCAATTCGAGCTCCTTGTAGTTCTATATTGTCTGCATCTTCGTTTTATTGGAAAAGCCGAAATCAGCTGAGCAGTACCAAGAAATGGCTATGCCAGATGCATGATTCAACTCCATCAGATGACGAGTATCGATCATCGAGGAACATAGCAATCAGTTTGTTCAGGCGGTACAGGAATGTGCTTGACCGTGGAGGAGGTGACAACCTAAAAGAGTTCATCAGTGCGGGGGTAAATGCGTATGCACTGGGATGCACAGATGAGGGATTGAGAAAAGAGCTTATTGATATGAAGCAATCTGGGGTTGAAATTGAAGCAATGGAGAGCTATAGTGGAAGCACCAGTTTGAAATCCAAGATTGCATCAGGGGAGATTGATGAGTGCATCCTGTGGTTGAGCATTATATTTATCACCATCTTGTGTACACCACAACCGACAATAGTTAGATGGTCATCCACACCTCCAGTGTCAGATGAAGAAAGGCTCAAGTGGAAAGGTTTTTGTGCGGTCATAGCAAATGCTTATTACTTGAGGGGAATGGCATGGCTCCCCGTAAAGACTCTTCAACTAGAGCAAATGGCAGTAGTTGGACAAGCTGAGGAACCATCTGTTGTTGCAAGTCGAATGCGATTAGTGTTTAGCACACTCGAGGTTGTGAGTCCACAGTGGCCTAAAGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

266

Amino Acids

29.65

Weight (kDa)

8.08

Isoelectric Point (pI)

60.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7876 PF25286 76 - 264 4.2e-106 Domain of unknown function (DUF7876)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 207
AciI CCGC 3 cut(s) 289, 347, 626
AcsI RAATTY 1 cut(s) 91
AfaI GTAC 3 cut(s) 199, 293, 544
AfiI CCNNNNNNNGG 1 cut(s) 207
AflIII ACRYGT 2 cut(s) 32, 85
AgsI TTSAA 6 cut(s) 111, 231, 428, 434, 469, 686
AluBI AGCT 6 cut(s) 26, 134, 190, 400, 447, 716
AluI AGCT 6 cut(s) 26, 134, 190, 400, 447, 716
Alw21I GWGCWC 1 cut(s) 136
Alw26I GTCTC 1 cut(s) 53
Ama87I CYCGRG 1 cut(s) 767
AoxI GGCC 2 cut(s) 121, 788
ApoI RAATTY 1 cut(s) 91
AspS9I GGNCC 1 cut(s) 122
AsuHPI GGTGA 2 cut(s) 332, 523
AvaI CYCGRG 1 cut(s) 767
BanII GRGCYC 1 cut(s) 136
BarI GAAGNNNNNNTAC 2 cut(s) 666, 698
Bbv12I GWGCWC 1 cut(s) 136
BbvCI CCTCAGC 1 cut(s) 717
BccI CCATC 4 cut(s) 244, 542, 561, 733
BcoDI GTCTC 1 cut(s) 53
BfaI CTAG 2 cut(s) 38, 689
BfmI CTRYAG 1 cut(s) 448
BlpI GCTNAGC 1 cut(s) 191
BmeT110I CYCGRG 1 cut(s) 767
BmgT120I GGNCC 1 cut(s) 122
BmiI GGNNCC 2 cut(s) 668, 723
BmrI ACTGGG 1 cut(s) 377
BmsI GCATC 5 cut(s) 167, 210, 362, 491, 513
BmuI ACTGGG 1 cut(s) 377
BpmI CTGGAG 1 cut(s) 567
Bpu10I CCTNAGC 1 cut(s) 717
Bpu1102I GCTNAGC 1 cut(s) 191
BpuEI CTTGAG 2 cut(s) 590, 670
Bsa29I ATCGAT 1 cut(s) 253
BsaBI GATNNNNATC 1 cut(s) 571
BsaJI CCNNGG 1 cut(s) 310
Bsc4I CCNNNNNNNGG 1 cut(s) 207
Bse1I ACTGG 3 cut(s) 372, 462, 584
Bse3DI GCAATG 1 cut(s) 444
Bse8I GATNNNNATC 1 cut(s) 571
BseCI ATCGAT 1 cut(s) 253
BseDI CCNNGG 1 cut(s) 310
BseGI GGATG 3 cut(s) 377, 504, 572
BseJI GATNNNNATC 1 cut(s) 571
BseLI CCNNNNNNNGG 1 cut(s) 207
BseMI GCAATG 1 cut(s) 444
BseMII CTCAG 2 cut(s) 182, 708
BseNI ACTGG 3 cut(s) 372, 462, 584
BseRI GAGGAG 1 cut(s) 330
BshFI GGCC 2 cut(s) 123, 790
BshVI ATCGAT 1 cut(s) 253
BsiHKAI GWGCWC 1 cut(s) 136
BsiHKCI CYCGRG 1 cut(s) 767
BslI CCNNNNNNNGG 1 cut(s) 207
BsmAI GTCTC 1 cut(s) 53
BsmI GAATGC 1 cut(s) 750
BsnI GGCC 2 cut(s) 123, 790
BsoBI CYCGRG 1 cut(s) 767
Bsp1286I GDGCHC 1 cut(s) 136
Bsp1407I TGTACA 1 cut(s) 542
Bsp143I GATC 1 cut(s) 254
Bsp1720I GCTNAGC 1 cut(s) 191
BspACI CCGC 3 cut(s) 289, 347, 626
BspANI GGCC 2 cut(s) 123, 790
BspCNI CTCAG 2 cut(s) 183, 709
BspDI ATCGAT 1 cut(s) 253
BspLI GGNNCC 2 cut(s) 668, 723
BsrDI GCAATG 1 cut(s) 444
BsrGI TGTACA 1 cut(s) 542
BsrI ACTGG 3 cut(s) 372, 462, 584
BssECI CCNNGG 1 cut(s) 310
BssMI GATC 1 cut(s) 254
Bst4CI ACNGT 2 cut(s) 311, 786
Bst6I CTCTTC 1 cut(s) 687
BstAUI TGTACA 1 cut(s) 542
BstDEI CTNAG 2 cut(s) 191, 717
BstDSI CCRYGG 1 cut(s) 310
BstF5I GGATG 3 cut(s) 377, 504, 572
BstKTI GATC 1 cut(s) 257
BstMAI GTCTC 1 cut(s) 53
BstMBI GATC 1 cut(s) 254
BstMWI GCNNNNNNNGC 2 cut(s) 23, 632
BstNSI RCATGY 2 cut(s) 36, 89
BstSFI CTRYAG 1 cut(s) 448
Bsu15I ATCGAT 1 cut(s) 253
BsuRI GGCC 2 cut(s) 123, 790
BsuTUI ATCGAT 1 cut(s) 253
BtgI CCRYGG 1 cut(s) 310
BtsCI GGATG 3 cut(s) 377, 504, 572
BtsIMutI CAGTG 4 cut(s) 349, 365, 591, 791
Cfr13I GGNCC 1 cut(s) 122
ClaI ATCGAT 1 cut(s) 253
Csp6I GTAC 3 cut(s) 198, 292, 543
CviAII CATG 4 cut(s) 33, 86, 224, 663
CviQI GTAC 3 cut(s) 198, 292, 543
DdeI CTNAG 2 cut(s) 191, 717
DpnI GATC 1 cut(s) 256
DpnII GATC 1 cut(s) 254
Eam1104I CTCTTC 1 cut(s) 687
EarI CTCTTC 1 cut(s) 687
Ecl136II GAGCTC 1 cut(s) 134
Eco24I GRGCYC 1 cut(s) 136
Eco53kI GAGCTC 1 cut(s) 134
Eco88I CYCGRG 1 cut(s) 767
EcoICRI GAGCTC 1 cut(s) 134
EcoRI GAATTC 1 cut(s) 91
EcoT22I ATGCAT 1 cut(s) 225
EcoT38I GRGCYC 1 cut(s) 136
FaeI CATG 4 cut(s) 36, 89, 227, 666
FatI CATG 4 cut(s) 32, 85, 223, 662
FauI CCCGC 1 cut(s) 340
FokI GGATG 3 cut(s) 384, 491, 559
FriOI GRGCYC 1 cut(s) 136
FspBI CTAG 2 cut(s) 38, 689
GsuI CTGGAG 1 cut(s) 567
HaeIII GGCC 2 cut(s) 123, 790
Hin1II CATG 4 cut(s) 36, 89, 227, 666
HinfI GANTC 4 cut(s) 41, 227, 679, 778
HphI GGTGA 2 cut(s) 332, 523
Hpy166II GTNNAC 3 cut(s) 543, 545, 782
Hpy188I TCNGA 2 cut(s) 241, 592
Hpy188III TCNNGA 1 cut(s) 38
Hpy8I GTNNAC 3 cut(s) 543, 545, 782
HpyCH4III ACNGT 2 cut(s) 311, 786
HpyCH4V TGCA 7 cut(s) 158, 223, 365, 375, 482, 504, 737
HpyF10VI GCNNNNNNNGC 2 cut(s) 23, 632
HpyF3I CTNAG 2 cut(s) 191, 717
Hsp92II CATG 4 cut(s) 36, 89, 227, 666
Kzo9I GATC 1 cut(s) 254
LmnI GCTCC 3 cut(s) 22, 139, 672
LweI GCATC 5 cut(s) 167, 210, 362, 491, 513
MaeI CTAG 2 cut(s) 38, 689
MaeIII GTNAC 2 cut(s) 28, 320
MalI GATC 1 cut(s) 256
MboI GATC 1 cut(s) 254
MboII GAAGA 3 cut(s) 153, 608, 674
MhlI GDGCHC 1 cut(s) 136
MluCI AATT 3 cut(s) 91, 126, 429
MlyI GAGTC 2 cut(s) 673, 787
MmeI TCCRAC 1 cut(s) 688
MnlI CCTC 8 cut(s) 255, 308, 311, 376, 591, 645, 712, 763
Mph1103I ATGCAT 1 cut(s) 225
MseI TTAA 1 cut(s) 98
MslI CAYNNNNRTG 1 cut(s) 370
MspA1I CMGCKG 1 cut(s) 190
Mva1269I GAATGC 1 cut(s) 750
MwoI GCNNNNNNNGC 2 cut(s) 23, 632
NdeII GATC 1 cut(s) 254
NlaIII CATG 4 cut(s) 36, 89, 227, 666
NlaIV GGNNCC 2 cut(s) 668, 723
NmuCI GTSAC 1 cut(s) 320
NsiI ATGCAT 1 cut(s) 225
NspI RCATGY 2 cut(s) 36, 89
PaeR7I CTCGAG 1 cut(s) 767
PciI ACATGT 2 cut(s) 32, 85
PctI GAATGC 1 cut(s) 750
PfeI GAWTC 2 cut(s) 41, 227
PflMI CCANNNNNTGG 1 cut(s) 207
PleI GAGTC 2 cut(s) 673, 786
PpsI GAGTC 2 cut(s) 673, 786
PscI ACATGT 2 cut(s) 32, 85
Psp124BI GAGCTC 1 cut(s) 136
PspN4I GGNNCC 2 cut(s) 668, 723
PspPI GGNCC 1 cut(s) 122
PspXI VCTCGAGB 1 cut(s) 767
PvuII CAGCTG 1 cut(s) 190
RsaI GTAC 3 cut(s) 199, 293, 544
RsaNI GTAC 3 cut(s) 198, 292, 543
RseI CAYNNNNRTG 1 cut(s) 370
SacI GAGCTC 1 cut(s) 136
SaqAI TTAA 1 cut(s) 98
Sau3AI GATC 1 cut(s) 254
Sau96I GGNCC 1 cut(s) 122
SchI GAGTC 2 cut(s) 673, 787
SduI GDGCHC 1 cut(s) 136
SfaNI GCATC 5 cut(s) 167, 210, 362, 491, 513
SfcI CTRYAG 1 cut(s) 448
Sfr274I CTCGAG 1 cut(s) 767
SlaI CTCGAG 1 cut(s) 767
SmiMI CAYNNNNRTG 1 cut(s) 370
SmlI CTYRAG 3 cut(s) 605, 649, 767
SmoI CTYRAG 3 cut(s) 605, 649, 767
Sse9I AATT 3 cut(s) 91, 126, 429
SsiI CCGC 3 cut(s) 289, 347, 626
SspMI CTAG 2 cut(s) 38, 689
SstI GAGCTC 1 cut(s) 136
TaaI ACNGT 2 cut(s) 311, 786
TaqI TCGA 5 cut(s) 130, 253, 260, 742, 768
TaqII GACCGA 1 cut(s) 104
TasI AATT 3 cut(s) 91, 126, 429
TatI WGTACW 1 cut(s) 542
TfiI GAWTC 2 cut(s) 41, 227
Tru1I TTAA 1 cut(s) 98
Tru9I TTAA 1 cut(s) 98
TscAI CASTG 4 cut(s) 349, 372, 591, 791
TseFI GTSAC 1 cut(s) 320
Tsp45I GTSAC 1 cut(s) 320
TspDTI ATGAA 3 cut(s) 328, 425, 609
TspRI CASTG 4 cut(s) 349, 372, 591, 791
Van91I CCANNNNNTGG 1 cut(s) 207
XapI RAATTY 1 cut(s) 91
XbaI TCTAGA 1 cut(s) 37
XceI RCATGY 2 cut(s) 36, 89
XhoI CTCGAG 1 cut(s) 767
XspI CTAG 2 cut(s) 38, 689
Zsp2I ATGCAT 1 cut(s) 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.