AT1G62850

RF-1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0008388
Physical Location & Seq
Reverse (-)
23271703 .. 23274340
2638 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G62850.3

Sequence Viewer

Length: 711 bp
ATGGCGGCAATCAGAACGATGACGAACATGATTCTACGCGAGTTCATCCATCATCCACTTCTCCTTCACTCTTCATCGAAATCGTGTCAATCTCTTTTACCATGTCTCCGTCTCACTCCCCTAATTTCTCCAATTCACTCTAATTCCCGGCTCGTATCCGTCCGATGTGCGGCATCGACCTCCGGTGGAAGCGGTGGTGATCGGAAAGTCTCTTCACGGTTGTCGCAGGTGCAACAGATGCTTCATGAAGCCGAAGAGAGAGCTAGTTCCGCTGGCAATGAGCCTACTCCTCAAATCACTCTAGACAATGTTACACTTAACTTTGCTAGAAGTGGTGGTCCTGGAGGCCAGAATGTGAACAAATTGAATACCAAAGTAGATATGCGCTTCAATGTTAAGAACGCGTATTGGCTGAGTGACAGGATCAGAGAGAAAATCTTACTGACGGAGAAGAATCGGATCAACAAGGATGGTGAACTTGTGATATCTTCTACCAAAACCAGAACGCAGAAAGGTAACATCGACGATGCACTTGAAAAACTACAGGCGATAATTGATGCGGCTTCATATGTTCCACCTCCACCATCAGAAGAACAAAAGAAGAAAATAGTAAAGCTCGCTGCGAAAGCTGACAATAAACGACTTAAAAGCAAAAAAGTTCTGTCAGACAAGAAATCTGCGAGAAGAAGCCGCGGTAGTTACGATGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RF-1

Protein Analysis

236

Amino Acids

26.07

Weight (kDa)

10.04

Isoelectric Point (pI)

46.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RF-1 PF00472 98 - 224 5e-15 RF-1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 217
Acc36I ACCTGC 1 cut(s) 217
AccII CGCG 3 cut(s) 39, 404, 693
AciI CCGC 7 cut(s) 5, 170, 192, 270, 560, 691, 693
AclWI GGATC 2 cut(s) 431, 467
AfiI CCNNNNNNNGG 1 cut(s) 169
AflIII ACRYGT 1 cut(s) 402
AgsI TTSAA 3 cut(s) 367, 391, 536
AjnI CCWGG 1 cut(s) 340
AluBI AGCT 3 cut(s) 263, 616, 629
AluI AGCT 3 cut(s) 263, 616, 629
Alw26I GTCTC 3 cut(s) 110, 116, 214
AlwI GGATC 2 cut(s) 431, 467
AoxI GGCC 1 cut(s) 346
ApeKI GCWGC 1 cut(s) 620
AspLEI GCGC 1 cut(s) 387
AspS9I GGNCC 1 cut(s) 338
AsuC2I CCSGG 1 cut(s) 148
AsuHPI GGTGA 2 cut(s) 209, 485
AvaII GGWCC 1 cut(s) 338
BbvI GCAGC 1 cut(s) 607
BccI CCATC 3 cut(s) 57, 464, 592
BciT130I CCWGG 1 cut(s) 342
BciVI GTATCC 1 cut(s) 166
BcnI CCSGG 1 cut(s) 148
BcoDI GTCTC 3 cut(s) 110, 116, 214
BfaI CTAG 3 cut(s) 264, 302, 327
BfmI CTRYAG 1 cut(s) 542
BfuAI ACCTGC 1 cut(s) 217
BfuI GTATCC 1 cut(s) 166
BisI GCNGC 5 cut(s) 6, 171, 561, 621, 691
BlsI GCNGC 5 cut(s) 7, 172, 562, 622, 692
Bme1390I CCNGG 2 cut(s) 148, 342
Bme18I GGWCC 1 cut(s) 338
BmgT120I GGNCC 1 cut(s) 338
BmrFI CCNGG 2 cut(s) 148, 342
BmsI GCATC 4 cut(s) 182, 228, 517, 547
BpmI CTGGAG 1 cut(s) 363
BpuMI CCSGG 1 cut(s) 148
BsaJI CCNNGG 1 cut(s) 691
BsaWI WCCGGW 1 cut(s) 182
BsaXI ACNNNNNCTCC 2 cut(s) 90, 120
Bsc4I CCNNNNNNNGG 1 cut(s) 169
Bse3DI GCAATG 1 cut(s) 283
BseBI CCWGG 1 cut(s) 342
BseDI CCNNGG 1 cut(s) 691
BseGI GGATG 3 cut(s) 45, 52, 475
BseLI CCNNNNNNNGG 1 cut(s) 169
BseMI GCAATG 1 cut(s) 283
BseMII CTCAG 1 cut(s) 404
BseRI GAGGAG 1 cut(s) 279
BseXI GCAGC 1 cut(s) 607
Bsh1236I CGCG 3 cut(s) 39, 404, 693
BshFI GGCC 1 cut(s) 348
BsiSI CCGG 2 cut(s) 148, 183
BslI CCNNNNNNNGG 1 cut(s) 169
BsmAI GTCTC 3 cut(s) 110, 116, 214
BsmBI CGTCTC 1 cut(s) 116
BsnI GGCC 1 cut(s) 348
Bsp143I GATC 3 cut(s) 199, 423, 459
BspACI CCGC 7 cut(s) 5, 170, 192, 270, 560, 691, 693
BspANI GGCC 1 cut(s) 348
BspCNI CTCAG 1 cut(s) 405
BspFNI CGCG 3 cut(s) 39, 404, 693
BspHI TCATGA 1 cut(s) 244
BspMI ACCTGC 1 cut(s) 217
BspPI GGATC 2 cut(s) 431, 467
BsrDI GCAATG 1 cut(s) 283
BssECI CCNNGG 1 cut(s) 691
BssMI GATC 3 cut(s) 199, 423, 459
Bst2UI CCWGG 1 cut(s) 342
Bst4CI ACNGT 1 cut(s) 219
Bst6I CTCTTC 3 cut(s) 76, 217, 249
BstAPI GCANNNNNTGC 1 cut(s) 238
BstC8I GCNNGC 2 cut(s) 274, 618
BstDEI CTNAG 1 cut(s) 413
BstDSI CCRYGG 1 cut(s) 691
BstF5I GGATG 3 cut(s) 45, 52, 475
BstFNI CGCG 3 cut(s) 39, 404, 693
BstHHI GCGC 1 cut(s) 387
BstKTI GATC 3 cut(s) 202, 426, 462
BstMAI GTCTC 3 cut(s) 110, 116, 214
BstMBI GATC 3 cut(s) 199, 423, 459
BstMWI GCNNNNNNNGC 3 cut(s) 238, 269, 626
BstNI CCWGG 1 cut(s) 342
BstSCI CCNGG 2 cut(s) 146, 340
BstSFI CTRYAG 1 cut(s) 542
BstUI CGCG 3 cut(s) 39, 404, 693
BstV1I GCAGC 1 cut(s) 607
BsuI GTATCC 1 cut(s) 166
BsuRI GGCC 1 cut(s) 348
BtgI CCRYGG 1 cut(s) 691
BtsCI GGATG 3 cut(s) 45, 52, 475
BveI ACCTGC 1 cut(s) 217
Cac8I GCNNGC 2 cut(s) 274, 618
CciI TCATGA 1 cut(s) 244
CfoI GCGC 1 cut(s) 387
Cfr13I GGNCC 1 cut(s) 338
Cfr42I CCGCGG 1 cut(s) 694
CviAII CATG 3 cut(s) 28, 102, 245
DdeI CTNAG 1 cut(s) 413
DpnI GATC 3 cut(s) 201, 425, 461
DpnII GATC 3 cut(s) 199, 423, 459
Eam1104I CTCTTC 3 cut(s) 76, 217, 249
EarI CTCTTC 3 cut(s) 76, 217, 249
Eco32I GATATC 1 cut(s) 486
Eco47I GGWCC 1 cut(s) 338
EcoRII CCWGG 1 cut(s) 340
EcoRV GATATC 1 cut(s) 486
Esp3I CGTCTC 1 cut(s) 116
FaeI CATG 3 cut(s) 31, 105, 248
FaiI YATR 6 cut(s) 29, 103, 246, 383, 568, 570
FatI CATG 3 cut(s) 27, 101, 244
FauNDI CATATG 1 cut(s) 568
Fnu4HI GCNGC 5 cut(s) 6, 171, 561, 621, 691
FokI GGATG 3 cut(s) 32, 39, 482
Fsp4HI GCNGC 5 cut(s) 6, 171, 561, 621, 691
FspBI CTAG 3 cut(s) 264, 302, 327
GlaI GCGC 1 cut(s) 386
GluI GCNGC 5 cut(s) 6, 171, 561, 621, 691
GsuI CTGGAG 1 cut(s) 363
HaeIII GGCC 1 cut(s) 348
HapII CCGG 2 cut(s) 148, 183
HhaI GCGC 1 cut(s) 387
Hin1II CATG 3 cut(s) 31, 105, 248
Hin6I GCGC 1 cut(s) 385
HinP1I GCGC 1 cut(s) 385
HinfI GANTC 2 cut(s) 31, 454
HpaII CCGG 2 cut(s) 148, 183
HphI GGTGA 2 cut(s) 209, 485
Hpy166II GTNNAC 2 cut(s) 358, 476
Hpy188I TCNGA 7 cut(s) 14, 164, 204, 428, 459, 589, 667
Hpy188III TCNNGA 2 cut(s) 245, 302
Hpy8I GTNNAC 2 cut(s) 358, 476
Hpy99I CGWCG 1 cut(s) 527
HpyAV CCTTC 1 cut(s) 74
HpyCH4III ACNGT 1 cut(s) 219
HpyCH4V TGCA 2 cut(s) 232, 530
HpyF10VI GCNNNNNNNGC 3 cut(s) 238, 269, 626
HpyF3I CTNAG 1 cut(s) 413
Hsp92II CATG 3 cut(s) 31, 105, 248
HspAI GCGC 1 cut(s) 385
KspI CCGCGG 1 cut(s) 694
Kzo9I GATC 3 cut(s) 199, 423, 459
Lsp1109I GCAGC 1 cut(s) 607
LweI GCATC 4 cut(s) 182, 228, 517, 547
MaeI CTAG 3 cut(s) 264, 302, 327
MaeIII GTNAC 4 cut(s) 310, 416, 515, 698
MalI GATC 3 cut(s) 201, 425, 461
MboI GATC 3 cut(s) 199, 423, 459
MboII GAAGA 8 cut(s) 63, 204, 266, 463, 480, 602, 613, 696
MluCI AATT 5 cut(s) 123, 132, 142, 362, 552
MluI ACGCGT 1 cut(s) 402
MnlI CCTC 4 cut(s) 190, 300, 338, 588
MseI TTAA 4 cut(s) 318, 396, 645, 709
MspA1I CMGCKG 2 cut(s) 272, 693
MspI CCGG 2 cut(s) 148, 183
MspR9I CCNGG 2 cut(s) 148, 342
MvaI CCWGG 1 cut(s) 342
MvnI CGCG 3 cut(s) 39, 404, 693
MwoI GCNNNNNNNGC 3 cut(s) 238, 269, 626
NciI CCSGG 1 cut(s) 148
NdeI CATATG 1 cut(s) 568
NdeII GATC 3 cut(s) 199, 423, 459
NlaIII CATG 3 cut(s) 31, 105, 248
NmuCI GTSAC 1 cut(s) 416
PagI TCATGA 1 cut(s) 244
PaqCI CACCTGC 1 cut(s) 217
PfeI GAWTC 2 cut(s) 31, 454
PfoI TCCNGGA 1 cut(s) 340
PkrI GCNGC 5 cut(s) 7, 172, 562, 622, 692
Psp6I CCWGG 1 cut(s) 340
PspGI CCWGG 1 cut(s) 340
PspPI GGNCC 1 cut(s) 338
SacII CCGCGG 1 cut(s) 694
SaqAI TTAA 4 cut(s) 318, 396, 645, 709
SatI GCNGC 5 cut(s) 6, 171, 561, 621, 691
Sau3AI GATC 3 cut(s) 199, 423, 459
Sau96I GGNCC 1 cut(s) 338
ScrFI CCNGG 2 cut(s) 148, 342
SetI ASST 7 cut(s) 182, 231, 265, 517, 580, 618, 631
SfaNI GCATC 4 cut(s) 182, 228, 517, 547
SfcI CTRYAG 1 cut(s) 542
Sfr303I CCGCGG 1 cut(s) 694
SgrBI CCGCGG 1 cut(s) 694
SinI GGWCC 1 cut(s) 338
Sse9I AATT 5 cut(s) 123, 132, 142, 362, 552
SsiI CCGC 7 cut(s) 5, 170, 192, 270, 560, 691, 693
SspMI CTAG 3 cut(s) 264, 302, 327
StyD4I CCNGG 2 cut(s) 146, 340
TaaI ACNGT 1 cut(s) 219
TaqI TCGA 3 cut(s) 77, 176, 522
TasI AATT 5 cut(s) 123, 132, 142, 362, 552
TauI GCSGC 4 cut(s) 8, 173, 563, 693
TfiI GAWTC 2 cut(s) 31, 454
Tru1I TTAA 4 cut(s) 318, 396, 645, 709
Tru9I TTAA 4 cut(s) 318, 396, 645, 709
TseFI GTSAC 1 cut(s) 416
TseI GCWGC 1 cut(s) 620
Tsp45I GTSAC 1 cut(s) 416
TspDTI ATGAA 5 cut(s) 34, 63, 233, 261, 555
TspGWI ACGGA 3 cut(s) 98, 148, 461
VpaK11BI GGWCC 1 cut(s) 338
XbaI TCTAGA 1 cut(s) 301
XspI CTAG 3 cut(s) 264, 302, 327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.