Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000007

Orthogroup ID

OG0008388

Physical Location & Seq

Genomic Coordinates Reverse (-)

4862479 .. 4874785

Gene Structure 12307 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug07G0064600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3624 bp

ATGGCTCAGATCTTCGAGCACTTCGTTGTCTGCGGTATCGGACCGGAGATCCGAACCCTCGACGGCGCAAGAGGCTTCCACGGCTTCGCCGAAATGTACCTGCCGTCTTTGCTCGACCAGTTCCCTCCCGCCAACCACACTCTCTACCCTCCTCCGCCGCCTCAGCTCCCCACCTGTGTGTTACCGGCTGGCGTTTCGTTCTACTCCTCAGGATTTGATTCCAGTGATGCGACGACGTTTCCACGGAGCTATCCCATTGTTCTAACTGAGGGTGATGGGTCAAAAATCTACGTTAGTTGCATTGCTTTCCGGGATCCGGTCAGTGAGGATATTGCTGAAGCTTATTGTATACCGGCAAATTCATTTGCTGACAAATGTATCTGTCTAGTTTCGCGGTCACCTAGTCTCCGTTTACTTAAAAATGTACTGGAGGAGTTGTTTGCACTGTGTTTTTCCTCTAGTGGAAGCAGCAAACCATTATGGGATATTATTGCGTCTATGGTATCCAATGTACCTCTGCCTACTCCTGGAAAAGATCGAGTTTTGTTTGCTATCGACAATTGTCTGCTTTCTGTGGAGACTCCACCGAAGGATGGGCTCCCTTATGTTGATATATCATTTCAGCCGTTGGTACAGTGTCTAGATGTTGACAACTTAATTAAATTTTTCACAGCCGTGCTGCTTGAAAGGAGGATTTTGATCCGATCACACAAATATTCACTTTTGACCCTTGCATCAGAAGCCATTTGCTATTTAATTTATCCATTTCGGTGGCAGCAAGTCTACATTCCGTTACTCTTTTTCAGTGGAGTAGACTATATTGATGCCCCTACGCCGTATATGATGGGTCTTCATTCTGGTGTTGATACATCTACTCTAGCAATGGATGGTGTAGTTATTGTGGACCTTGAGTTCAATCGTATCACAACAACAGAAGATATACCTTCTATTCCCGAGCCAGAACTAAGCTGTTTGCGTCAGGAAATAACGCAGCTATTGTATCCCAATGTTGTGGGGATAGATCTGATGAAGGATGGTGTTTTCAGTTCATCCGAGCAATATCCTAAACTTGGCAACAAACCTTGGGGAGAGGATCATGACCTACAACTTAGGCTAGTGTTCTTAAAGTTCTTTGCATCACTTTTAAGTGGCTATCGGAACTTCATAGAACTTTCTGCTACTCACGTCTTCAATGTTCAAGCATTCTTGAAGATGCGCTCTCGGTCAACTCAGCAACCCCCAGAACCCATGTTGACACAATTTTTGGAATCACATGGTTTCATGGATTATCTGGAAAGAGGTTTGGGTTCTGATGAAAACAACAATAATCTACTTGACAAGTTACAAGATGCACTGGGAAGGGGCCAAAATCCTATTTCAATTCTTCCTTCAAATTCAGTTGAGCCTGAGATTATAACAATTGCGGACACAGATGTGGGAACATCAGGGTCAGGTGGCACATACACTTATGACAGGTTTCCCTCAAACATTAGGACAGAAGAGGAAGAAGAAAAGAGGAAGCAAATTCTTGCTGCAGCAAGTGGTTTATCTGCTAGCTCACCTTCTGTCCTTCCTGGTAAAGACCCCAAATCAGAAACTCTAACTCCATTGGAGCGGGCTGCTGAAAGGGAATGTATGGTTTTGGACATAAAAGTTAAGCTGCAGGGTCTGTGGCTGCGTCTTCTAAAACTGGGACCTACTGATGATCCTCTTTCTTCATTTGAATATGGAACAATCCTTGCTTTAATTGAATCTGATGCAGAAGGAATTGGTGGCAGTGGGTTTGTTGAATGTATAAGGGAGCACATAAATTCGGGATGGCACTGTCAGTTGACTGAAGAACAATTTATTGCTGTGAAGGAACTGCTGAAAACAGCTATCAGTCGTGCAACGTCCAGAAATGATTGGATGACCATCAGAGATGCCCTTGAAGTGTCTGCTGAAATGTTTAAGAAGGATGGAAATAATGTTTCAGATTATGTTCAGCGTCATCTTATATCTTTGTCCATTTGGGAGGAATTACGTTTCTGGGAAAGCTACTTTGACTGTCTAATGGAACGGTCTTCAAACAAGTCGGCCAACTATGCCTCTTTGGTGACTGATCAGCTATCTTTGGTGGAATTACACATGGCTGGACTAGGACTTCCTGATCTTGATGCATGGTACATGATTGAAACAATAGCTGAGAAGAACAACATTGGATACACACAATTTATAAAGCTGAGGGGGTTATTGTCGCATGTTCAACAACTACGAATTGGTTACTGGGGAATCAGTTCTTTCAAGGCCCAATCCATGTCTCATGGATTAGCATCTCCACGTTCAAAAGATGCCGCAGTTGAGAGTCAGCAACCTGCCGAGGCCTCCGGTGTTGGGAGGAGCTGGGTTCAGAGCATGTTCAGCAGGGATAATTCAACCAGATTAAACTCTTTCAGTCGTGTTCGCAAATGGACTTCTGATGGTGGCTCTTCAGCTACAAATGAAAATGGGACATCTCGTAAGCGAGAATTATCTGCAGCTGGACAGAAGAAAACCCAAACCAATGTTCGAGTACTTAGAGGCCATACTGGCCCTATCACTGCTCTACATTGTGTAACAAGGAGGGAAGTTTGGGATCTAGTGGGTGACCGTGAAGACGCAGGTTTCTTCATCAGTGGGAGCACAGACTGCACGGTTAAGATCTGGGATCCTAGTTTTCGTGGTAACGAACTTCGGGCAACCCTGAAAGGACACACAAGAACCATCCGTGCAATAAGTTCGGATCGAGGAAAGGTGGTATCTGGATCAGATGATCAATCTGTTTTAGTATGGGATAAGCAAACTACTCAGCTTCTAGAAGAGTTGAAAGGCCATGATGCACAGATTAGCTGTGCTCGCATGCTGTCAGGGGAACGTGTCCTTACTGCTGCTCATGATGGAACTGTTAAAATGTGGGATGTTAGAACTGATACTTGTGTGGCAACTGTTGGTCGTTGCTCTAGTGCCGTCCTATGCATGGAATATGATGACTCCACAGGAATCCTAGCTGCTGGTGGTAGAGATGCCATTGCTAACATTTGGGACATTCGTGCCGGTAGGCAAATGCACAAGCTTCTAGGGCACACAAAATGGATCCGGTCAATTAGAATGGTGGGGGACACAGTGGTTACTGGTAGTGATGATTGGACTGCAAGAATGTGGTCCATTTCCAGAGGAACATGTGATGCTGTTCTTGCATGCCATGCTGGCCCAATATTATCCGTTGAGTACTCCACACTTGACAGAGGAATAATCACAGGTTCAACAGATGGCTTACTTCGCTTTTGGGAAAATGTGGAAGGAGGTATAAGATGTGTAAAAAATGTTACTGTTCACACTGCTGCTATATTGTCCATCAATGCTGCGGAACATTGGTTAGGAATCGGAGCAGCTGATAATTCCATGTCTCTTTTTCATCGGCCTCAAGAGAAACTTGGCGGCTTCTCTAATACGGGATCAAAAATGGCTGGGTGGCAACTATACCGAACACCACAAAGAACAGCTGCTGTGGTAAGATGCGTTGCTACGGACCTTGAGAGAAAAAGGATATGCAGTGGTGATCGCAAGGGCCAGCTTCGGCTATGGGAAGCCACCATAAACATCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000313 GO:0000315 GO:0003674 GO:0003676 GO:0003723 GO:0003747 GO:0003824 GO:0004045 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0005761 GO:0005762 GO:0005840 GO:0006412 GO:0006414 GO:0006415 GO:0006417 GO:0006518 GO:0006807 GO:0008079 GO:0008135 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010608 GO:0015934 GO:0016043 GO:0016150 GO:0016787 GO:0016788 GO:0019222 GO:0019538 GO:0022411 GO:0031323 GO:0031326 GO:0031974 GO:0032268 GO:0032543 GO:0032984 GO:0032991 GO:0034248 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043603 GO:0043604 GO:0043624 GO:0043933 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0050789 GO:0050794 GO:0051171 GO:0051246 GO:0052689 GO:0060255 GO:0065007 GO:0070013 GO:0070126 GO:0071704 GO:0071840 GO:0072344 GO:0080090 GO:0097159 GO:0098798 GO:0140053 GO:0140098 GO:0140101 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1990904 GO:2000112

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RF-1

Protein Analysis

1207

Amino Acids

133.72

Weight (kDa)

5.69

Isoelectric Point (pI)

43.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
uDENN	PF03456	35 - 103	2.2e-12	uDENN domain
DENN	PF02141	123 - 304	1.3e-42	DENN (AEX-3) domain
Beta-prop_WDR5	PF25175	879 - 1031	2.4e-27	WDR5 beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	879 - 979	7.3e-09	WDR3 second beta-propeller domain
WD40_MABP1-WDR62_2nd	PF24782	880 - 1045	3.1e-07	MABP1/WDR62 second WD40 domain
Beta-prop_EML_2	PF23414	882 - 1030	4.2e-11	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st	PF25173	882 - 993	1.5e-21	WDR3 first beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	882 - 1078	1.9e-16	TEP-1 second beta-propeller
Beta-prop_THOC3	PF25174	883 - 1103	7.2e-31	THOC3 beta-propeller domain
WD40_WDHD1_1st	PF24817	883 - 988	3.8e-09	WDHD1 first WD40 domain
WD40_MABP1-WDR62_1st	PF24780	884 - 1014	2e-06	MABP1/WDR62 first WD40 domain
WDR55	PF24796	885 - 1103	2e-19	WDR55
Beta-prop_WDR36-Utp21_1st	PF25171	899 - 1102	2.1e-14	WDR36/Utp21 first beta-propeller
EIF3I	PF24805	907 - 1001	8.9e-07	EIF3I
Beta-prop_WDR41	PF25178	907 - 1014	3.5e-09	WDR41
WD40_Prp19	PF24814	920 - 996	1e-10	Prp19 WD40 domain
WD40_CDC20-Fz	PF24807	924 - 1067	2.6e-14	CDC20/Fizzy WD40 domain
WD40_Gbeta	PF25391	948 - 1144	1.2e-15	G protein beta WD-40 repeat protein
Beta-prop_DCAF4	PF23761	951 - 1027	6.3e-07	DDB1- and CUL4-associated factor 4 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	964 - 1066	2.8e-07	WDR36/Utp21 second beta-propeller domain
WD40_WDHD1_1st	PF24817	988 - 1067	1.5e-06	WDHD1 first WD40 domain
Beta-prop_WDR3_2nd	PF25172	992 - 1151	1.9e-10	WDR3 second beta-propeller domain
Beta-prop_WDR5	PF25175	993 - 1126	3.9e-20	WDR5 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	994 - 1103	2.5e-19	WDR3 first beta-propeller domain
WD40_Prp19	PF24814	995 - 1115	1.3e-14	Prp19 WD40 domain
Beta-prop_THOC3	PF25174	995 - 1203	2.7e-13	THOC3 beta-propeller domain
WD40_WDHD1_1st	PF24817	1045 - 1118	8e-07	WDHD1 first WD40 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0008388)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G62850 AT1G62850 AT1G62850
fragaria_vesca	FvH4_5g00690 FvH4_5g00690 FvH4_5g00690
malus_domestica	MD04G1079400.v1.1
prunus_persica	Prupe.5G079700_v2.0.a1 Prupe.5G079700_v2.0.a1 Prupe.5G079700_v2.0.a1
pyrus_communis	pycom04g07120
rosa_chinensis	RchiOBHm_Chr7g0201521
rosa_laevigata	RLG00000003665
rosa_multiflora	Rmu_sc0002046.1_g000001 Rmu_sc0017656.1_g000001
rosa_roxburghii	Rroxscaffold_3G00255250
rosa_rugosa	Rorug07G0064600 Rorug07G0064700
rosa_samantha	Rh7AG191200 Rh7BG193500 Rh7CG202400 Rh7DG196400
rosa_wichuraiana	Rw7G016760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1415, 2216
Acc36I	ACCTGC	3 cut(s)	108, 2362, 2630
AccBSI	CCGCTC	1 cut(s)	1615
AccI	GTMKAC	3 cut(s)	349, 783, 813
AccII	CGCG	1 cut(s)	394
AcoI	YGGCCR	1 cut(s)	2076
AcsI	RAATTY	5 cut(s)	358, 662, 1393, 1524, 1810
AcuI	CTGAAG	3 cut(s)	357, 1857, 2454
AfaI	GTAC	7 cut(s)	98, 426, 513, 633, 2165, 2553, 3248
AfiI	CCNNNNNNNGG	6 cut(s)	316, 527, 593, 1070, 2373, 2995
AflIII	ACRYGT	2 cut(s)	2893, 3197
AjiI	CACGTC	1 cut(s)	1186
AjnI	CCWGG	2 cut(s)	526, 1573
AjuI	GAANNNNNNNTTGG	2 cut(s)	2529, 2561
AleI	CACNNNNGTG	3 cut(s)	176, 674, 1433
AloI	GAACNNNNNNTCC	2 cut(s)	896, 928
Alw21I	GWGCWC	4 cut(s)	21, 1806, 2663, 2875
Alw26I	GTCTC	4 cut(s)	410, 572, 2304, 3429
AlwNI	CAGNNNCTG	2 cut(s)	1669, 3524
Ama87I	CYCGRG	1 cut(s)	953
ApoI	RAATTY	5 cut(s)	358, 662, 1393, 1524, 1810
ArsI	GACNNNNNNTTYG	2 cut(s)	1246, 1278
Asp700I	GAANNNNTTC	1 cut(s)	2275
AspLEI	GCGC	2 cut(s)	68, 1218
AsuC2I	CCSGG	1 cut(s)	311
AsuHPI	GGTGA	6 cut(s)	284, 390, 1551, 2107, 2636, 3587
AsuNHI	GCTAGC	1 cut(s)	1553
AvaI	CYCGRG	1 cut(s)	953
AvaII	GGWCC	5 cut(s)	41, 904, 1694, 3180, 3547
AxyI	CCTNAGG	1 cut(s)	208
BaeGI	GKGCMC	1 cut(s)	3102
BaeI	ACNNNNGTAYC	4 cut(s)	361, 394, 2940, 2973
BamHI	GGATCC	3 cut(s)	313, 2686, 3111
BanII	GRGCYC	1 cut(s)	600
BbsI	GAAGAC	5 cut(s)	842, 1180, 1673, 2055, 2640
Bbv12I	GWGCWC	4 cut(s)	21, 1806, 2663, 2875
BbvCI	CCTCAGC	2 cut(s)	162, 2222
BceAI	ACGGC	7 cut(s)	79, 88, 97, 610, 659, 820, 2969
BcgI	CGANNNNNNTGC	2 cut(s)	2739, 2773
BciT130I	CCWGG	2 cut(s)	528, 1575
BciVI	GTATCC	3 cut(s)	514, 1011, 2195
BclI	TGATCA	2 cut(s)	2101, 2791
BcnI	CCSGG	1 cut(s)	311
BcoDI	GTCTC	4 cut(s)	410, 572, 2304, 3429
BfmI	CTRYAG	3 cut(s)	1533, 1661, 2514
BfuAI	ACCTGC	3 cut(s)	108, 2362, 2630
BfuI	GTATCC	3 cut(s)	514, 1011, 2195
BglI	GCCNNNNNGGC	2 cut(s)	2568, 3225
BglII	AGATCT	3 cut(s)	9, 1021, 2679
BmcAI	AGTACT	2 cut(s)	2553, 3248
Bme1390I	CCNGG	3 cut(s)	311, 528, 1575
Bme18I	GGWCC	5 cut(s)	41, 904, 1694, 3180, 3547
BmeT110I	CYCGRG	1 cut(s)	953
BmgBI	CACGTC	1 cut(s)	1186
BmiI	GGNNCC	6 cut(s)	315, 599, 1364, 1695, 2688, 3113
BmrFI	CCNGG	3 cut(s)	311, 528, 1575
BmrI	ACTGGG	3 cut(s)	1364, 1700, 2275
BmtI	GCTAGC	1 cut(s)	1557
BmuI	ACTGGG	3 cut(s)	1364, 1700, 2275
BoxI	GACNNNNGTC	1 cut(s)	561
BpiI	GAAGAC	5 cut(s)	842, 1180, 1673, 2055, 2640
BpmI	CTGGAG	1 cut(s)	449
Bpu10I	CCTNAGC	2 cut(s)	162, 2222
BpuEI	CTTGAG	3 cut(s)	929, 3426, 3572
BpuMI	CCSGG	1 cut(s)	311
BsaBI	GATNNNNATC	3 cut(s)	698, 1913, 2311
BsaJI	CCNNGG	4 cut(s)	79, 242, 1082, 2358
BsaWI	WCCGGW	4 cut(s)	43, 316, 2366, 3114
BsaXI	ACNNNNNCTCC	6 cut(s)	390, 420, 422, 452, 1588, 1618
Bsc4I	CCNNNNNNNGG	6 cut(s)	316, 527, 593, 1070, 2373, 2995
Bse118I	RCCGGY	3 cut(s)	184, 352, 3071
Bse1I	ACTGG	8 cut(s)	118, 222, 432, 1359, 1695, 2270, 2572, 3154
Bse21I	CCTNAGG	1 cut(s)	208
Bse3DI	GCAATG	3 cut(s)	300, 888, 3045
Bse8I	GATNNNNATC	3 cut(s)	698, 1913, 2311
BseBI	CCWGG	2 cut(s)	528, 1575
BseDI	CCNNGG	4 cut(s)	79, 242, 1082, 2358
BseGI	GGATG	9 cut(s)	598, 892, 1039, 1049, 1823, 1914, 1963, 2742, 2941
BseJI	GATNNNNATC	3 cut(s)	698, 1913, 2311
BseLI	CCNNNNNNNGG	6 cut(s)	316, 527, 593, 1070, 2373, 2995
BseMI	GCAATG	3 cut(s)	300, 888, 3045
BseMII	CTCAG	9 cut(s)	20, 176, 222, 258, 1244, 1398, 2175, 2213, 2840
BseNI	ACTGG	8 cut(s)	118, 222, 432, 1359, 1695, 2270, 2572, 3154
BseRI	GAGGAG	4 cut(s)	141, 196, 446, 2392
BseSI	GKGCMC	1 cut(s)	3102
BseYI	CCCAGC	2 cut(s)	2382, 3485
BsgI	GTGCAG	1 cut(s)	2653
Bsh1236I	CGCG	1 cut(s)	394
BsiHKAI	GWGCWC	4 cut(s)	21, 1806, 2663, 2875
BsiHKCI	CYCGRG	1 cut(s)	953
BsiSI	CCGG	8 cut(s)	44, 185, 310, 317, 353, 2367, 3072, 3115
BslFI	GGGAC	4 cut(s)	1707, 2503, 3074, 3149
BslI	CCNNNNNNNGG	6 cut(s)	316, 527, 593, 1070, 2373, 2995
BsmAI	GTCTC	4 cut(s)	410, 572, 2304, 3429
BsmFI	GGGAC	4 cut(s)	1707, 2503, 3074, 3149
BsmI	GAATGC	1 cut(s)	1202
BsoBI	CYCGRG	1 cut(s)	953
Bsp1286I	GDGCHC	6 cut(s)	21, 600, 1806, 2663, 2875, 3102
BspCNI	CTCAG	9 cut(s)	19, 175, 221, 259, 1243, 1399, 2176, 2214, 2839
BspFNI	CGCG	1 cut(s)	394
BspHI	TCATGA	2 cut(s)	1096, 2911
BspLI	GGNNCC	6 cut(s)	315, 599, 1364, 1695, 2688, 3113
BspMAI	CTGCAG	3 cut(s)	1537, 1665, 2518
BspMI	ACCTGC	3 cut(s)	108, 2362, 2630
BspOI	GCTAGC	1 cut(s)	1557
BspQI	GCTCTTC	1 cut(s)	2473
BsrBI	CCGCTC	1 cut(s)	1615
BsrDI	GCAATG	3 cut(s)	300, 888, 3045
BsrFI	RCCGGY	3 cut(s)	184, 352, 3071
BsrI	ACTGG	8 cut(s)	118, 222, 432, 1359, 1695, 2270, 2572, 3154
BssAI	RCCGGY	3 cut(s)	184, 352, 3071
BssECI	CCNNGG	4 cut(s)	79, 242, 1082, 2358
BssNAI	GTATAC	1 cut(s)	350
BssT1I	CCWWGG	1 cut(s)	1082
Bst1107I	GTATAC	1 cut(s)	350
Bst2UI	CCWGG	2 cut(s)	528, 1575
Bst6I	CTCTTC	3 cut(s)	1494, 2473, 2832
BstAPI	GCANNNNNTGC	2 cut(s)	2667, 3221
BstC8I	GCNNGC	8 cut(s)	190, 1555, 1617, 2875, 2879, 3217, 3226, 3590
BstDSI	CCRYGG	2 cut(s)	79, 242
BstEII	GGTNACC	2 cut(s)	396, 2624
BstF5I	GGATG	9 cut(s)	598, 892, 1039, 1049, 1823, 1914, 1963, 2742, 2941
BstFNI	CGCG	1 cut(s)	394
BstHHI	GCGC	2 cut(s)	68, 1218
BstMAI	GTCTC	4 cut(s)	410, 572, 2304, 3429
BstNI	CCWGG	2 cut(s)	528, 1575
BstNSI	RCATGY	5 cut(s)	2243, 2398, 2881, 3201, 3219
BstPAI	GACNNNNGTC	1 cut(s)	561
BstPI	GGTNACC	2 cut(s)	396, 2624
BstSCI	CCNGG	3 cut(s)	309, 526, 1573
BstSFI	CTRYAG	3 cut(s)	1533, 1661, 2514
BstSLI	GKGCMC	1 cut(s)	3102
BstUI	CGCG	1 cut(s)	394
BstV2I	GAAGAC	5 cut(s)	842, 1180, 1673, 2055, 2640
BstX2I	RGATCY	8 cut(s)	9, 48, 313, 1021, 2614, 2679, 2686, 3111
BstXI	CCANNNNNNTGG	2 cut(s)	771, 1012
BstYI	RGATCY	8 cut(s)	9, 48, 313, 1021, 2614, 2679, 2686, 3111
BstZ17I	GTATAC	1 cut(s)	350
Bsu36I	CCTNAGG	1 cut(s)	208
BsuI	GTATCC	3 cut(s)	514, 1011, 2195
BtgI	CCRYGG	2 cut(s)	79, 242
BtrI	CACGTC	1 cut(s)	1186
BtsCI	GGATG	9 cut(s)	598, 892, 1039, 1049, 1823, 1914, 1963, 2742, 2941
BtsI	GCAGTG	4 cut(s)	1783, 2577, 3354, 3577
BveI	ACCTGC	3 cut(s)	108, 2362, 2630
Cac8I	GCNNGC	8 cut(s)	190, 1555, 1617, 2875, 2879, 3217, 3226, 3590
CaiI	CAGNNNCTG	2 cut(s)	1669, 3524
CciI	TCATGA	2 cut(s)	1096, 2911
CfoI	GCGC	2 cut(s)	68, 1218
Cfr10I	RCCGGY	3 cut(s)	184, 352, 3071
CpoI	CGGWCCG	1 cut(s)	41
CseI	GACGC	5 cut(s)	483, 965, 1667, 1976, 2645
Csp6I	GTAC	7 cut(s)	97, 425, 512, 632, 2164, 2552, 3247
CspI	CGGWCCG	1 cut(s)	41
CviQI	GTAC	7 cut(s)	97, 425, 512, 632, 2164, 2552, 3247
EaeI	YGGCCR	1 cut(s)	2076
Eam1104I	CTCTTC	3 cut(s)	1494, 2473, 2832
EarI	CTCTTC	3 cut(s)	1494, 2473, 2832
EciI	GGCGGA	1 cut(s)	144
Eco130I	CCWWGG	1 cut(s)	1082
Eco147I	AGGCCT	1 cut(s)	2363
Eco24I	GRGCYC	1 cut(s)	600
Eco47I	GGWCC	5 cut(s)	41, 904, 1694, 3180, 3547
Eco57I	CTGAAG	3 cut(s)	357, 1857, 2454
Eco81I	CCTNAGG	1 cut(s)	208
Eco88I	CYCGRG	1 cut(s)	953
Eco91I	GGTNACC	2 cut(s)	396, 2624
EcoO109I	RGGNCCY	1 cut(s)	1694
EcoO65I	GGTNACC	2 cut(s)	396, 2624
EcoRII	CCWGG	2 cut(s)	526, 1573
EcoT14I	CCWWGG	1 cut(s)	1082
EcoT22I	ATGCAT	2 cut(s)	2161, 2996
EcoT38I	GRGCYC	1 cut(s)	600
ErhI	CCWWGG	1 cut(s)	1082
FalI	AAGNNNNNCTT	2 cut(s)	3435, 3467
FaqI	GGGAC	4 cut(s)	1707, 2503, 3074, 3149
FauI	CCCGC	2 cut(s)	136, 1608
FbaI	TGATCA	2 cut(s)	2101, 2791
FblI	GTMKAC	3 cut(s)	349, 783, 813
FokI	GGATG	9 cut(s)	605, 899, 1036, 1046, 1830, 1921, 1970, 2729, 2948
FriOI	GRGCYC	1 cut(s)	600
GlaI	GCGC	2 cut(s)	67, 1217
GsaI	CCCAGC	2 cut(s)	2386, 3489
GsuI	CTGGAG	1 cut(s)	449
HapII	CCGG	8 cut(s)	44, 185, 310, 317, 353, 2367, 3072, 3115
HgaI	GACGC	5 cut(s)	483, 965, 1667, 1976, 2645
HhaI	GCGC	2 cut(s)	68, 1218
Hin6I	GCGC	2 cut(s)	66, 1216
HinP1I	GCGC	2 cut(s)	66, 1216
HincII	GTYRAC	4 cut(s)	649, 1227, 1254, 1833
HindII	GTYRAC	4 cut(s)	649, 1227, 1254, 1833
HindIII	AAGCTT	2 cut(s)	339, 3089
HinfI	GANTC	9 cut(s)	218, 580, 1268, 1751, 2271, 2344, 3008, 3018, 3399
HpaII	CCGG	8 cut(s)	44, 185, 310, 317, 353, 2367, 3072, 3115
HphI	GGTGA	6 cut(s)	284, 390, 1551, 2107, 2636, 3587
Hpy99I	CGWCG	3 cut(s)	65, 235, 238
HpyCH4IV	ACGT	7 cut(s)	236, 291, 1185, 1892, 2023, 2320, 2893
HpySE526I	ACGT	7 cut(s)	236, 291, 1185, 1892, 2023, 2320, 2893
HspAI	GCGC	2 cut(s)	66, 1216
Ksp22I	TGATCA	2 cut(s)	2101, 2791
LguI	GCTCTTC	1 cut(s)	2473
LmnI	GCTCC	8 cut(s)	171, 246, 603, 1612, 1801, 2379, 2658, 3404
MaeII	ACGT	7 cut(s)	236, 291, 1185, 1892, 2023, 2320, 2893
MbiI	CCGCTC	1 cut(s)	1615
MfeI	CAATTG	2 cut(s)	559, 1419
MflI	RGATCY	8 cut(s)	9, 48, 313, 1021, 2614, 2679, 2686, 3111
MhlI	GDGCHC	6 cut(s)	21, 600, 1806, 2663, 2875, 3102
MlyI	GAGTC	3 cut(s)	574, 2353, 3002
Mph1103I	ATGCAT	2 cut(s)	2161, 2996
MroXI	GAANNNNTTC	1 cut(s)	2275
MslI	CAYNNNNRTG	5 cut(s)	176, 674, 769, 858, 1433
MspA1I	CMGCKG	3 cut(s)	2519, 3410, 3521
MspI	CCGG	8 cut(s)	44, 185, 310, 317, 353, 2367, 3072, 3115
MspR9I	CCNGG	3 cut(s)	311, 528, 1575
MunI	CAATTG	2 cut(s)	559, 1419
Mva1269I	GAATGC	1 cut(s)	1202
MvaI	CCWGG	2 cut(s)	528, 1575
MvnI	CGCG	1 cut(s)	394
NciI	CCSGG	1 cut(s)	311
NheI	GCTAGC	1 cut(s)	1553
NlaIV	GGNNCC	6 cut(s)	315, 599, 1364, 1695, 2688, 3113
NmeAIII	GCCGAG	1 cut(s)	2383
NmuCI	GTSAC	3 cut(s)	396, 2095, 2624
NsiI	ATGCAT	2 cut(s)	2161, 2996
NspI	RCATGY	5 cut(s)	2243, 2398, 2881, 3201, 3219
OliI	CACNNNNGTG	3 cut(s)	176, 674, 1433
PacI	TTAATTAA	1 cut(s)	660
PaeI	GCATGC	2 cut(s)	2881, 3219
PagI	TCATGA	2 cut(s)	1096, 2911
PceI	AGGCCT	1 cut(s)	2363
PciI	ACATGT	1 cut(s)	3197
PciSI	GCTCTTC	1 cut(s)	2473
PcsI	WCGNNNNNNNCGW	2 cut(s)	21, 87
PctI	GAATGC	1 cut(s)	1202
PdmI	GAANNNNTTC	1 cut(s)	2275
PfeI	GAWTC	6 cut(s)	218, 1268, 1751, 2271, 3018, 3399
PfoI	TCCNGGA	2 cut(s)	309, 526
PleI	GAGTC	3 cut(s)	574, 2352, 3002
PpsI	GAGTC	3 cut(s)	574, 2352, 3002
PpuMI	RGGWCCY	1 cut(s)	1694
PscI	ACATGT	1 cut(s)	3197
PshAI	GACNNNNGTC	1 cut(s)	561
PsiI	TTATAA	2 cut(s)	1415, 2216
Psp5II	RGGWCCY	1 cut(s)	1694
Psp6I	CCWGG	2 cut(s)	526, 1573
PspEI	GGTNACC	2 cut(s)	396, 2624
PspFI	CCCAGC	2 cut(s)	2382, 3485
PspGI	CCWGG	2 cut(s)	526, 1573
PspN4I	GGNNCC	6 cut(s)	315, 599, 1364, 1695, 2688, 3113
PspPPI	RGGWCCY	1 cut(s)	1694
PstI	CTGCAG	3 cut(s)	1537, 1665, 2518
PstNI	CAGNNNCTG	2 cut(s)	1669, 3524
PsuI	RGATCY	8 cut(s)	9, 48, 313, 1021, 2614, 2679, 2686, 3111
PvuII	CAGCTG	3 cut(s)	2519, 3410, 3521
RsaI	GTAC	7 cut(s)	98, 426, 513, 633, 2165, 2553, 3248
RsaNI	GTAC	7 cut(s)	97, 425, 512, 632, 2164, 2552, 3247
RseI	CAYNNNNRTG	5 cut(s)	176, 674, 769, 858, 1433
Rsr2I	CGGWCCG	1 cut(s)	41
RsrII	CGGWCCG	1 cut(s)	41
SapI	GCTCTTC	1 cut(s)	2473
ScaI	AGTACT	2 cut(s)	2553, 3248
SchI	GAGTC	3 cut(s)	574, 2353, 3002
ScrFI	CCNGG	3 cut(s)	311, 528, 1575
SduI	GDGCHC	6 cut(s)	21, 600, 1806, 2663, 2875, 3102
SfcI	CTRYAG	3 cut(s)	1533, 1661, 2514
SfiI	GGCCNNNNNGGCC	1 cut(s)	2568
SinI	GGWCC	5 cut(s)	41, 904, 1694, 3180, 3547
SmiMI	CAYNNNNRTG	5 cut(s)	176, 674, 769, 858, 1433
SmlI	CTYRAG	3 cut(s)	908, 3441, 3551
SmoI	CTYRAG	3 cut(s)	908, 3441, 3551
SphI	GCATGC	2 cut(s)	2881, 3219
SseBI	AGGCCT	1 cut(s)	2363
SspI	AATATT	2 cut(s)	716, 3234
StuI	AGGCCT	1 cut(s)	2363
StyD4I	CCNGG	3 cut(s)	309, 526, 1573
StyI	CCWWGG	1 cut(s)	1082
TaiI	ACGT	7 cut(s)	239, 294, 1188, 1895, 2026, 2323, 2896
TaqI	TCGA	7 cut(s)	15, 60, 114, 538, 555, 2548, 2764
TaqII	GACCGA	1 cut(s)	1212
TatI	WGTACW	3 cut(s)	424, 2551, 3246
TauI	GCSGC	3 cut(s)	160, 2336, 3459
TfiI	GAWTC	6 cut(s)	218, 1268, 1751, 2271, 3018, 3399
TseFI	GTSAC	3 cut(s)	396, 2095, 2624
Tsp45I	GTSAC	3 cut(s)	396, 2095, 2624
TspGWI	ACGGA	6 cut(s)	259, 398, 780, 2735, 3229, 3560
VpaK11BI	GGWCC	5 cut(s)	41, 904, 1694, 3180, 3547
XapI	RAATTY	5 cut(s)	358, 662, 1393, 1524, 1810
XbaI	TCTAGA	2 cut(s)	640, 2833
XceI	RCATGY	5 cut(s)	2243, 2398, 2881, 3201, 3219
XmiI	GTMKAC	3 cut(s)	349, 783, 813
XmnI	GAANNNNTTC	1 cut(s)	2275
ZrmI	AGTACT	2 cut(s)	2553, 3248
Zsp2I	ATGCAT	2 cut(s)	2161, 2996

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.