AT1G65032

protein polyubiquitination

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0013108
Physical Location & Seq
Reverse (-)
24158650 .. 24159635
986 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G65032.1

Sequence Viewer

Length: 276 bp
ATGAAGAGTCTGATATGGTTAACAGCAGAAGAGGCTGCAAAGAACAGAGGAAAAGTTCTTTCTTTGTATCGACAATTACTTAGGAGCATTAACTCGCCAAAATTGCAGCTTAGTTATGCTTCAAGACTTGCGAAGAAAGCTGAGGTTAGGACCATCTTCTTGTTTGGCTCTGAGGAGATTTCGAAACACAATGTCGCTGATCTCATCCGTACTGGTGAATATGCTCTTTCTCAATTGAAACAAGGCAAAATCCCCAACAACTCTACTCAGTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

91

Amino Acids

10.32

Weight (kDa)

10.06

Isoelectric Point (pI)

41.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LYRM_2 PF23655 12 - 80 1.7e-35 LYR motif containing domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfaI GTAC 1 cut(s) 211
AgsI TTSAA 2 cut(s) 123, 238
AluBI AGCT 2 cut(s) 109, 140
AluI AGCT 2 cut(s) 109, 140
ApeKI GCWGC 2 cut(s) 35, 106
AspS9I GGNCC 1 cut(s) 150
AsuHPI GGTGA 1 cut(s) 227
AsuII TTCGAA 1 cut(s) 182
AvaII GGWCC 1 cut(s) 150
BbvCI CCTCAGC 1 cut(s) 141
BbvI GCAGC 2 cut(s) 22, 118
BccI CCATC 1 cut(s) 161
BisI GCNGC 2 cut(s) 36, 107
BlsI GCNGC 2 cut(s) 37, 108
Bme18I GGWCC 1 cut(s) 150
BmgT120I GGNCC 1 cut(s) 150
Bpu10I CCTNAGC 1 cut(s) 141
Bpu14I TTCGAA 1 cut(s) 182
Bse1I ACTGG 1 cut(s) 217
BseGI GGATG 1 cut(s) 204
BseMII CTCAG 2 cut(s) 132, 162
BseNI ACTGG 1 cut(s) 217
BseRI GAGGAG 1 cut(s) 188
BseXI GCAGC 2 cut(s) 22, 118
Bsp119I TTCGAA 1 cut(s) 182
Bsp143I GATC 1 cut(s) 199
BspCNI CTCAG 2 cut(s) 133, 163
BspT104I TTCGAA 1 cut(s) 182
BsrI ACTGG 1 cut(s) 217
BssMI GATC 1 cut(s) 199
Bst6I CTCTTC 1 cut(s) 24
BstBI TTCGAA 1 cut(s) 182
BstDEI CTNAG 5 cut(s) 80, 110, 141, 171, 267
BstF5I GGATG 1 cut(s) 204
BstKTI GATC 1 cut(s) 202
BstMBI GATC 1 cut(s) 199
BstMWI GCNNNNNNNGC 3 cut(s) 32, 103, 137
BstV1I GCAGC 2 cut(s) 22, 118
BtsCI GGATG 1 cut(s) 204
Cfr13I GGNCC 1 cut(s) 150
Csp6I GTAC 1 cut(s) 210
CviJI RGCY 4 cut(s) 35, 109, 140, 168
CviKI_1 RGCY 4 cut(s) 35, 109, 140, 168
CviQI GTAC 1 cut(s) 210
DdeI CTNAG 5 cut(s) 80, 110, 141, 171, 267
DpnI GATC 1 cut(s) 201
DpnII GATC 1 cut(s) 199
Eam1104I CTCTTC 1 cut(s) 24
EarI CTCTTC 1 cut(s) 24
Eco47I GGWCC 1 cut(s) 150
FaiI YATR 3 cut(s) 16, 117, 222
Fnu4HI GCNGC 2 cut(s) 36, 107
FokI GGATG 1 cut(s) 191
Fsp4HI GCNGC 2 cut(s) 36, 107
GluI GCNGC 2 cut(s) 36, 107
HincII GTYRAC 1 cut(s) 21
HindII GTYRAC 1 cut(s) 21
HinfI GANTC 1 cut(s) 7
HpaI GTTAAC 1 cut(s) 21
HphI GGTGA 1 cut(s) 227
Hpy166II GTNNAC 1 cut(s) 21
Hpy188I TCNGA 2 cut(s) 12, 172
Hpy188III TCNNGA 1 cut(s) 123
Hpy8I GTNNAC 1 cut(s) 21
HpyCH4V TGCA 2 cut(s) 38, 106
HpyF10VI GCNNNNNNNGC 3 cut(s) 32, 103, 137
HpyF3I CTNAG 5 cut(s) 80, 110, 141, 171, 267
KspAI GTTAAC 1 cut(s) 21
Kzo9I GATC 1 cut(s) 199
LmnI GCTCC 1 cut(s) 84
LpnPI CCDG 1 cut(s) 198
Lsp1109I GCAGC 2 cut(s) 22, 118
MalI GATC 1 cut(s) 201
MboI GATC 1 cut(s) 199
MboII GAAGA 4 cut(s) 16, 41, 145, 148
MfeI CAATTG 1 cut(s) 233
MluCI AATT 3 cut(s) 74, 101, 233
MlyI GAGTC 1 cut(s) 16
MnlI CCTC 4 cut(s) 25, 41, 136, 166
MseI TTAA 2 cut(s) 20, 90
MunI CAATTG 1 cut(s) 233
MwoI GCNNNNNNNGC 3 cut(s) 32, 103, 137
NdeII GATC 1 cut(s) 199
NspV TTCGAA 1 cut(s) 182
PkrI GCNGC 2 cut(s) 37, 108
PleI GAGTC 1 cut(s) 15
PpsI GAGTC 1 cut(s) 15
PspPI GGNCC 1 cut(s) 150
RsaI GTAC 1 cut(s) 211
RsaNI GTAC 1 cut(s) 210
SaqAI TTAA 2 cut(s) 20, 90
SatI GCNGC 2 cut(s) 36, 107
Sau3AI GATC 1 cut(s) 199
Sau96I GGNCC 1 cut(s) 150
SchI GAGTC 1 cut(s) 16
SetI ASST 3 cut(s) 111, 142, 147
SfuI TTCGAA 1 cut(s) 182
SgeI CNNG 6 cut(s) 106, 135, 140, 172, 225, 254
SinI GGWCC 1 cut(s) 150
Sse9I AATT 3 cut(s) 74, 101, 233
TaqI TCGA 2 cut(s) 70, 182
TasI AATT 3 cut(s) 74, 101, 233
Tru1I TTAA 2 cut(s) 20, 90
Tru9I TTAA 2 cut(s) 20, 90
TseI GCWGC 2 cut(s) 35, 106
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 197
VpaK11BI GGWCC 1 cut(s) 150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.