MD02G1240300.v1.1

protein polyubiquitination

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0013108
Physical Location & Seq
Reverse (-)
28884256 .. 28885593
1338 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1240300.v1.1.491

Sequence Viewer

Length: 273 bp
ATGGTGCAGGGGGTAATTTGGGCGACGGCGGAGGACTTGGCAAGAAACAGAGGACGCGTCCTCTCTCTGTATCGCCAGATACTCAGAAGCCTCAACTCGACGAGCTTGCCGCTGAATCTGGCCGCAAGGCTGGCCAAGAAAGCGGAGGCGCACGCAATCTTCATGTTGGCGGCGGAGGAGAGATCCCTGCACAACATTGACGACCTCGTTGATGCTGCTGACTACTCTCTCTCCCTCTTGAGGAAGGGGGAATTACCCAAGTACATTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

91

Amino Acids

10.08

Weight (kDa)

9.22

Isoelectric Point (pI)

37.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LYRM_2 PF23655 12 - 81 2.9e-39 LYR motif containing domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 57
AciI CCGC 6 cut(s) 29, 110, 123, 143, 170, 173
AclWI GGATC 1 cut(s) 177
AcoI YGGCCR 2 cut(s) 120, 132
AfaI GTAC 1 cut(s) 263
AfiI CCNNNNNNNGG 1 cut(s) 240
AflIII ACRYGT 1 cut(s) 55
AgsI TTSAA 1 cut(s) 269
AluBI AGCT 1 cut(s) 105
AluI AGCT 1 cut(s) 105
AlwI GGATC 1 cut(s) 177
AoxI GGCC 2 cut(s) 120, 132
ApeKI GCWGC 1 cut(s) 215
AspLEI GCGC 1 cut(s) 151
BalI TGGCCA 1 cut(s) 134
BbvI GCAGC 1 cut(s) 202
BceAI ACGGC 1 cut(s) 42
BcgI CGANNNNNNTGC 2 cut(s) 88, 122
BisI GCNGC 4 cut(s) 110, 123, 171, 216
BlsI GCNGC 4 cut(s) 111, 124, 172, 217
BmsI GCATC 1 cut(s) 202
BpuEI CTTGAG 1 cut(s) 259
BsaXI ACNNNNNCTCC 2 cut(s) 215, 245
Bsc4I CCNNNNNNNGG 1 cut(s) 240
BseLI CCNNNNNNNGG 1 cut(s) 240
BseMII CTCAG 1 cut(s) 97
BseRI GAGGAG 1 cut(s) 191
BseXI GCAGC 1 cut(s) 202
BsgI GTGCAG 2 cut(s) 26, 173
Bsh1236I CGCG 1 cut(s) 57
BshFI GGCC 2 cut(s) 122, 134
BslI CCNNNNNNNGG 1 cut(s) 240
BsnI GGCC 2 cut(s) 122, 134
Bsp143I GATC 1 cut(s) 182
BspACI CCGC 6 cut(s) 29, 110, 123, 143, 170, 173
BspANI GGCC 2 cut(s) 122, 134
BspCNI CTCAG 1 cut(s) 96
BspFNI CGCG 1 cut(s) 57
BspPI GGATC 1 cut(s) 177
BssMI GATC 1 cut(s) 182
BstC8I GCNNGC 3 cut(s) 107, 132, 153
BstDEI CTNAG 1 cut(s) 83
BstFNI CGCG 1 cut(s) 57
BstHHI GCGC 1 cut(s) 151
BstKTI GATC 1 cut(s) 185
BstMBI GATC 1 cut(s) 182
BstMWI GCNNNNNNNGC 2 cut(s) 131, 140
BstUI CGCG 1 cut(s) 57
BstV1I GCAGC 1 cut(s) 202
BstX2I RGATCY 1 cut(s) 182
BstYI RGATCY 1 cut(s) 182
BsuRI GGCC 2 cut(s) 122, 134
Cac8I GCNNGC 3 cut(s) 107, 132, 153
CfoI GCGC 1 cut(s) 151
CseI GACGC 2 cut(s) 46, 63
Csp6I GTAC 1 cut(s) 262
CviAII CATG 1 cut(s) 163
CviJI RGCY 5 cut(s) 90, 105, 122, 130, 134
CviKI_1 RGCY 5 cut(s) 90, 105, 122, 130, 134
CviQI GTAC 1 cut(s) 262
DdeI CTNAG 1 cut(s) 83
DpnI GATC 1 cut(s) 184
DpnII GATC 1 cut(s) 182
EaeI YGGCCR 2 cut(s) 120, 132
EciI GGCGGA 2 cut(s) 44, 188
FaeI CATG 1 cut(s) 166
FaiI YATR 1 cut(s) 164
FatI CATG 1 cut(s) 162
Fnu4HI GCNGC 4 cut(s) 110, 123, 171, 216
Fsp4HI GCNGC 4 cut(s) 110, 123, 171, 216
GlaI GCGC 1 cut(s) 150
GluI GCNGC 4 cut(s) 110, 123, 171, 216
HaeIII GGCC 2 cut(s) 122, 134
HgaI GACGC 2 cut(s) 46, 63
HhaI GCGC 1 cut(s) 151
Hin1II CATG 1 cut(s) 166
Hin6I GCGC 1 cut(s) 149
HinP1I GCGC 1 cut(s) 149
HinfI GANTC 1 cut(s) 115
Hpy188I TCNGA 1 cut(s) 86
Hpy188III TCNNGA 1 cut(s) 238
Hpy99I CGWCG 2 cut(s) 28, 103
HpyAV CCTTC 1 cut(s) 238
HpyCH4V TGCA 2 cut(s) 7, 190
HpyF10VI GCNNNNNNNGC 2 cut(s) 131, 140
HpyF3I CTNAG 1 cut(s) 83
Hsp92II CATG 1 cut(s) 166
HspAI GCGC 1 cut(s) 149
Kzo9I GATC 1 cut(s) 182
LpnPI CCDG 4 cut(s) 89, 104, 116, 200
Lsp1109I GCAGC 1 cut(s) 202
LweI GCATC 1 cut(s) 202
MalI GATC 1 cut(s) 184
MboI GATC 1 cut(s) 182
MboII GAAGA 1 cut(s) 151
MflI RGATCY 1 cut(s) 182
MlsI TGGCCA 1 cut(s) 134
MluCI AATT 2 cut(s) 15, 251
MluI ACGCGT 1 cut(s) 55
MluNI TGGCCA 1 cut(s) 134
MnlI CCTC 9 cut(s) 25, 44, 71, 101, 139, 169, 215, 234, 245
Mox20I TGGCCA 1 cut(s) 134
MscI TGGCCA 1 cut(s) 134
Msp20I TGGCCA 1 cut(s) 134
MspA1I CMGCKG 1 cut(s) 112
MvnI CGCG 1 cut(s) 57
MwoI GCNNNNNNNGC 2 cut(s) 131, 140
NdeII GATC 1 cut(s) 182
NlaIII CATG 1 cut(s) 166
PfeI GAWTC 1 cut(s) 115
PkrI GCNGC 4 cut(s) 111, 124, 172, 217
PsuI RGATCY 1 cut(s) 182
RsaI GTAC 1 cut(s) 263
RsaNI GTAC 1 cut(s) 262
SatI GCNGC 4 cut(s) 110, 123, 171, 216
Sau3AI GATC 1 cut(s) 182
SetI ASST 2 cut(s) 107, 207
SfaNI GCATC 1 cut(s) 202
SmlI CTYRAG 1 cut(s) 238
SmoI CTYRAG 1 cut(s) 238
Sse9I AATT 2 cut(s) 15, 251
SsiI CCGC 6 cut(s) 29, 110, 123, 143, 170, 173
TaqI TCGA 1 cut(s) 98
TasI AATT 2 cut(s) 15, 251
TatI WGTACW 1 cut(s) 261
TauI GCSGC 3 cut(s) 112, 125, 173
TfiI GAWTC 1 cut(s) 115
TseI GCWGC 1 cut(s) 215
TspDTI ATGAA 1 cut(s) 151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.