Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0005119

Physical Location & Seq

Genomic Coordinates Forward (+)

25255155 .. 25260590

Gene Structure 5436 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G67420.3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2619 bp

ATGCCGTTTTGGAAGATGAGCGCAAGAGATGTCACTGGATTCAAATTCCTCTTCTCACTCGTCTTCATCTACGCTTTAATGTCAGCTATTGTTTACTCCGTCCTCCACATGAAATTCATCTCTCCGCTTCCGGCAAACGCTCCTCTCGAACGATTCTCTGAAGCTAGAGCCGTCGAACACATCCGTGTCTTGGCTGAAGAGATCGATGGTCGTCAGGAAGGACGACCTGGACTTAAAGAAGCTGCAACGTATATAAAATCGCAATTGGAGATGGTGAAGGAGAGAGCTGGACCAAATTTAAGGGTTGAAGTGGAAGAGACTCAAGTGGATGGTTCCTTTAGCATGATGTTTCTAGGACACAGCATATCACTTGGATACAGAAACCATACTAATATACTTATGCGTATCTCGTCAATGAATTCTCTTGACACTGATGCATCTGTATTGATGAATGCACATTATGACAGTCCTGTTAACTCTCCTGGAGCTGGTGATTGTGGGTCATGTGTAGCATCACTACTTGAATTAGCAAGACTGGTAGTGGATTCTGGTTGGGTCCCGCCTCAACCTGTAATTTTTCTCTTCAATGGTGCTGAAGAGCTTTTTATGCTGGGCTCACACGGCTTCATGACGAAGCATAAGTTGAAAGATACCATTGGAGCTTTTATAAACGTGGAAGCCTCTGGGACAGGGGGTATTGATCTGGTCTGCCAATCAGGGCCTGGCTCGTGGCCATCTTATGTCTACTCTCAAGCAGCAGTGTATCCAATGGCACAGAGTTCTGCACAGGATGTTTTCCCTGTAATTCCTGGAGACACAGATTACAGAATGTTTGCAGAAGATTATGCCGACATTCCCGGGCTAGACATTATCTTTCTTCTGGGCGGTTACTACTACCATACTACCTTCGATACAGTGGACAGAATAGTACCTGGAAGCATGCAAGCACGTGGAGAAAACTTAATCAGCGTGCTTAAAGCCTTCGCGAGTTCTTCTAGGCTAAAGGTTGCTAGTGAAAGAAAGACGCTTGACGTGGATGCCAACAGTGACATGGTTGAAAGAGCTGTTTTCTTTGATTACTTAACATGGTTCATGGTGTTCTACCCAAGAAGAGTAGCTTTCGTACTTCACAACATTCCAGCTGCTTTGTTCCTTTGTGTTCCTTTCTTTTTGTATATGATGGACCCTAGGACACACCCTTTGTTATCATTCTTCTGGGCTTTCTTTAAAGGAGTTATGCACCATTTCGCCGGGATCTTACTTGGTGTAATAGTCCCTGTTCTCTTTGCAGTCATCAGATTGTTCTTTGCTTATCCCATGAGCTGGTTTGCTCATTCATACTTAGCTTTCTTGATGTTCATCCCCTGCTCATTTTTTGGCCTTTTAATTCCAAGAGCTATCTCTGATCGTGTCTCACATTTTCAAGGTGTTTCATCCAAGAAAATTATGAAAGAACCATCTGATGAAGCAAGGTTTTGGGGAGCATTTGGGTTCTATGCTTTTGCAACTTCGGCATATTTTTTCGCTGGATTAAATGGAGGATTCATGACATTTGTCATCTCCATTTCTATGCTACTAGGGTGGATTGCTTTCTATTTGTCAGTCAAGTCCTATGGCTATAATTCGATCAAGTCTCCCATGTTTTATGTGATAGCTCTTGTTCCATGCCTTCTGTATTCTCTCTATTTTGGTGGTATTCTTACACTATTATTAATTGAGAAGACGGGAATGATGGGAGCAATTCCACCACCTTATGGATTTTATCTAGCAGATGTAGCAGTAGCTGCAGTCATTGGAATCGTCACCGGCTTGTGTTTGGGTCCAATAATACCAATTTGTGATCGTTGGCTGGCCAAGTCGTCGATCTTGAAATTCTTGCTTCACTTCACTGTAGTCATGTTGGCGGTATCATCACAGTTCTTTCCTTATAGCAAAGATGCACCAAAGAGAGTTGTACTCCAACACACATTCATCTCTACTGGTGGAAATGAAATCACAGGCTCAAGTTATGATTTAGCTGTCATTGATTCAAATTCTATGGAGTTCATTTTTAAGCATGCTCCGGAGGTGGCAAAGGAACTTCATGTTGGCCCTTCTTTTTCATTGGGAAACGCTGAAGCGTCTCCTCAAGAAGCTTGGATGGCACTTTTCCCTATTTCATGTGTATTAACAACAAACGGGAGGTTCCCTGCTAAAGCCAACAATATTTTAGAACGATATAGCCAATTCCCACTCCTACAAGCGCATAAACCGCAAACAACATTTGAAAATGGAACTCGCAGAGTTCATCTGGAGCTCTCTCTGGGGTCGTTGGAGGAGATTTGGGTCACGGTTTTGAACATAACCGGACCGTTATCAGGGTGGTCCTTTGCCGATGGCAAACCTCCAGCACCTGAACTCCCACCGGGTGGTCCTCCCTCGTACATATTGAGACTGAGTGGCAACAGCGATGAGAAGTGGAACTTCTGGTTAGAGGCAAGTAGTGAAGAAGAAGTGAGAGTAGATGTAGCTGTTCTTGATCAACGTCTTGATGAAGAAACTATACATTTGAAGGGTCTTTTCCCAAAATGGTCCGATGTCATTGCCTACACTAGTTTTCTTTCTACTTACTTGTTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001541 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0007275 GO:0007548 GO:0008150 GO:0008406 GO:0008585 GO:0012505 GO:0022414 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0045137 GO:0046545 GO:0046660 GO:0048513 GO:0048608 GO:0048731 GO:0048856 GO:0061458

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_M28

Protein Analysis

872

Amino Acids

96.99

Weight (kDa)

5.86

Isoelectric Point (pI)

40.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M28	PF04389	41 - 233	2.7e-44	Peptidase family M28
ERMP1_C	PF22248	558 - 781	3.6e-28	Endoplasmic reticulum metallopeptidase 1, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005119)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G67420 AT1G67420 AT1G67420 AT1G67420 AT1G67420 AT1G67420
fragaria_vesca	FvH4_4g23910 FvH4_4g23910 FvH4_4g23910 FvH4_4g23910 FvH4_4g23910 FvH4_4g23910
malus_domestica	MD13G1117700.v1.1 MD16G1117900.v1.1
prunus_persica	Prupe.1G227300_v2.0.a1 Prupe.1G227300_v2.0.a1 Prupe.1G227300_v2.0.a1
pyrus_communis	pycom13g10180 pycom16g10100
rosa_chinensis	RchiOBHm_Chr4g0430551
rosa_laevigata	RLG00000006970
rosa_multiflora	Rmu_sc0011782.1_g000005
rosa_roxburghii	Rroxscaffold_5G00372320
rosa_rugosa	Rorug04G0244100 Rorug04G0244200
rosa_samantha	Rh4AG300200 Rh4BG307300 Rh4CG324000 Rh4DG304600
rosa_wichuraiana	Rw4G026050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	668
AccB7I	CCANNNNNTGG	3 cut(s)	1323, 1754, 2408
AccI	GTMKAC	1 cut(s)	744
AccII	CGCG	1 cut(s)	986
AccIII	TCCGGA	1 cut(s)	2062
AciI	CCGC	5 cut(s)	125, 560, 885, 1904, 2252
AclWI	GGATC	1 cut(s)	1262
AcoI	YGGCCR	2 cut(s)	731, 1851
AcsI	RAATTY	6 cut(s)	44, 113, 295, 418, 1871, 2032
AcuI	CTGAAG	4 cut(s)	180, 216, 615, 2136
AcvI	CACGTG	1 cut(s)	950
AdeI	CACNNNGTG	1 cut(s)	2408
AfaI	GTAC	4 cut(s)	930, 1125, 1956, 2423
AfiI	CCNNNNNNNGG	6 cut(s)	190, 488, 1323, 1754, 2357, 2408
AhlI	ACTAGT	1 cut(s)	2591
AjiI	CACGTC	1 cut(s)	1033
AjnI	CCWGG	5 cut(s)	226, 481, 721, 808, 931
AjuI	GAANNNNNNNTTGG	2 cut(s)	2070, 2102
AleI	CACNNNNGTG	1 cut(s)	183
Alw21I	GWGCWC	1 cut(s)	2298
Alw26I	GTCTC	6 cut(s)	311, 807, 1417, 1638, 2127, 2425
AlwI	GGATC	1 cut(s)	1262
AlwNI	CAGNNNCTG	3 cut(s)	722, 1784, 2393
Ama87I	CYCGRG	1 cut(s)	857
Aor13HI	TCCGGA	1 cut(s)	2062
AoxI	GGCC	5 cut(s)	719, 731, 1378, 1851, 2089
ApeKI	GCWGC	4 cut(s)	242, 755, 1142, 1784
ApoI	RAATTY	6 cut(s)	44, 113, 295, 418, 1871, 2032
ArsI	GACNNNNNNTTYG	2 cut(s)	1183, 1215
AseI	ATTAAT	1 cut(s)	1712
AspA2I	CCTAGG	1 cut(s)	1187
AspLEI	GCGC	2 cut(s)	23, 2245
AsuC2I	CCSGG	4 cut(s)	858, 859, 1252, 2406
AsuHPI	GGTGA	3 cut(s)	286, 503, 1795
AvaI	CYCGRG	1 cut(s)	857
AvaII	GGWCC	8 cut(s)	290, 556, 1183, 1820, 2348, 2364, 2411, 2571
AvrII	CCTAGG	1 cut(s)	1187
BalI	TGGCCA	2 cut(s)	733, 1853
BanII	GRGCYC	2 cut(s)	617, 2298
BarI	GAAGNNNNNNTAC	2 cut(s)	2526, 2558
BauI	CACGAG	1 cut(s)	727
BbrPI	CACGTG	1 cut(s)	950
BbsI	GAAGAC	2 cut(s)	55, 1727
Bbv12I	GWGCWC	1 cut(s)	2298
BbvI	GCAGC	4 cut(s)	229, 767, 1129, 1771
BccI	CCATC	9 cut(s)	200, 265, 323, 742, 1174, 1465, 1726, 2134, 2369
BceAI	ACGGC	2 cut(s)	155, 637
BcgI	CGANNNNNNTGC	2 cut(s)	2564, 2598
BciT130I	CCWGG	5 cut(s)	228, 483, 723, 810, 933
BciVI	GTATCC	2 cut(s)	368, 774
BclI	TGATCA	1 cut(s)	2518
BcnI	CCSGG	4 cut(s)	858, 859, 1252, 2406
BcoDI	GTCTC	6 cut(s)	311, 807, 1417, 1638, 2127, 2425
BcuI	ACTAGT	1 cut(s)	2591
BfaI	CTAG	9 cut(s)	165, 353, 863, 996, 1011, 1188, 1577, 1766, 2592
BfmI	CTRYAG	2 cut(s)	1785, 1890
BfuI	GTATCC	2 cut(s)	368, 774
BisI	GCNGC	4 cut(s)	243, 756, 1143, 1785
BlnI	CCTAGG	1 cut(s)	1187
BlsI	GCNGC	4 cut(s)	244, 757, 1144, 1786
Bme1390I	CCNGG	9 cut(s)	228, 483, 723, 810, 858, 859, 933, 1252, 2406
Bme18I	GGWCC	8 cut(s)	290, 556, 1183, 1820, 2348, 2364, 2411, 2571
BmeT110I	CYCGRG	1 cut(s)	857
BmgBI	CACGTC	1 cut(s)	1033
BmiI	GGNNCC	6 cut(s)	334, 557, 558, 1185, 1821, 2186
BmrFI	CCNGG	9 cut(s)	228, 483, 723, 810, 858, 859, 933, 1252, 2406
BmsI	GCATC	5 cut(s)	424, 446, 521, 1027, 1927
BoxI	GACNNNNGTC	1 cut(s)	1553
BpiI	GAAGAC	2 cut(s)	55, 1727
BplI	GAGNNNNNCTC	2 cut(s)	1941, 1973
BpmI	CTGGAG	4 cut(s)	504, 831, 2312, 2370
BpuEI	CTTGAG	4 cut(s)	306, 735, 1987, 2112
BpuMI	CCSGG	4 cut(s)	858, 859, 1252, 2406
Bsa29I	ATCGAT	1 cut(s)	204
BsaAI	YACGTR	1 cut(s)	950
BsaBI	GATNNNNATC	1 cut(s)	1358
BsaJI	CCNNGG	2 cut(s)	857, 1187
BsaWI	WCCGGW	2 cut(s)	2062, 2345
BsaXI	ACNNNNNCTCC	2 cut(s)	2382, 2412
Bsc4I	CCNNNNNNNGG	6 cut(s)	190, 488, 1323, 1754, 2357, 2408
Bse118I	RCCGGY	1 cut(s)	1805
Bse1I	ACTGG	3 cut(s)	40, 540, 1984
Bse3DI	GCAATG	1 cut(s)	2580
Bse8I	GATNNNNATC	1 cut(s)	1358
BseAI	TCCGGA	1 cut(s)	2062
BseBI	CCWGG	5 cut(s)	228, 483, 723, 810, 933
BseCI	ATCGAT	1 cut(s)	204
BseDI	CCNNGG	2 cut(s)	857, 1187
BseGI	GGATG	7 cut(s)	180, 334, 796, 1042, 1359, 1433, 2145
BseJI	GATNNNNATC	1 cut(s)	1358
BseLI	CCNNNNNNNGG	6 cut(s)	190, 488, 1323, 1754, 2357, 2408
BseMI	GCAATG	1 cut(s)	2580
BseMII	CTCAG	1 cut(s)	2426
BseNI	ACTGG	3 cut(s)	40, 540, 1984
BseRI	GAGGAG	3 cut(s)	132, 2115, 2330
BseXI	GCAGC	4 cut(s)	229, 767, 1129, 1771
BseYI	CCCAGC	1 cut(s)	610
BsgI	GTGCAG	1 cut(s)	768
Bsh1236I	CGCG	1 cut(s)	986
BshFI	GGCC	5 cut(s)	721, 733, 1380, 1853, 2091
BshVI	ATCGAT	1 cut(s)	204
BsiHKAI	GWGCWC	1 cut(s)	2298
BsiHKCI	CYCGRG	1 cut(s)	857
BsiSI	CCGG	7 cut(s)	131, 858, 1251, 1806, 2063, 2346, 2405
BslFI	GGGAC	3 cut(s)	542, 700, 1259
BslI	CCNNNNNNNGG	6 cut(s)	190, 488, 1323, 1754, 2357, 2408
BsmAI	GTCTC	6 cut(s)	311, 807, 1417, 1638, 2127, 2425
BsmBI	CGTCTC	1 cut(s)	2127
BsmFI	GGGAC	3 cut(s)	542, 700, 1259
BsmI	GAATGC	1 cut(s)	457
BsnI	GGCC	5 cut(s)	721, 733, 1380, 1853, 2091
BsoBI	CYCGRG	1 cut(s)	857
Bsp1286I	GDGCHC	2 cut(s)	617, 2298
Bsp13I	TCCGGA	1 cut(s)	2062
Bsp143I	GATC	8 cut(s)	201, 700, 1254, 1405, 1626, 1840, 1863, 2518
Bsp68I	TCGCGA	1 cut(s)	986
BspACI	CCGC	5 cut(s)	125, 560, 885, 1904, 2252
BspANI	GGCC	5 cut(s)	721, 733, 1380, 1853, 2091
BspCNI	CTCAG	1 cut(s)	2427
BspDI	ATCGAT	1 cut(s)	204
BspEI	TCCGGA	1 cut(s)	2062
BspFNI	CGCG	1 cut(s)	986
BspHI	TCATGA	2 cut(s)	627, 1545
BspLI	GGNNCC	6 cut(s)	334, 557, 558, 1185, 1821, 2186
BspMAI	CTGCAG	1 cut(s)	1789
BspPI	GGATC	1 cut(s)	1262
BspQI	GCTCTTC	1 cut(s)	591
BsrDI	GCAATG	1 cut(s)	2580
BsrFI	RCCGGY	1 cut(s)	1805
BsrI	ACTGG	3 cut(s)	40, 540, 1984
BssAI	RCCGGY	1 cut(s)	1805
BssECI	CCNNGG	2 cut(s)	857, 1187
BssMI	GATC	8 cut(s)	201, 700, 1254, 1405, 1626, 1840, 1863, 2518
BssSI	CACGAG	1 cut(s)	727
BssT1I	CCWWGG	1 cut(s)	1187
Bst2BI	CACGAG	1 cut(s)	727
Bst2UI	CCWGG	5 cut(s)	228, 483, 723, 810, 933
Bst4CI	ACNGT	7 cut(s)	467, 916, 1046, 1891, 1917, 2332, 2352
Bst6I	CTCTTC	6 cut(s)	56, 192, 309, 587, 591, 1105
BstAPI	GCANNNNNTGC	1 cut(s)	1784
BstBAI	YACGTR	1 cut(s)	950
BstC8I	GCNNGC	5 cut(s)	941, 945, 971, 1851, 2058
BstDEI	CTNAG	2 cut(s)	1342, 2435
BstF5I	GGATG	7 cut(s)	180, 334, 796, 1042, 1359, 1433, 2145
BstFNI	CGCG	1 cut(s)	986
BstHHI	GCGC	2 cut(s)	23, 2245
BstKTI	GATC	8 cut(s)	204, 703, 1257, 1408, 1629, 1843, 1866, 2521
BstMAI	GTCTC	6 cut(s)	311, 807, 1417, 1638, 2127, 2425
BstMBI	GATC	8 cut(s)	201, 700, 1254, 1405, 1626, 1840, 1863, 2518
BstMWI	GCNNNNNNNGC	7 cut(s)	607, 621, 1511, 1775, 1784, 2141, 2251
BstNI	CCWGG	5 cut(s)	228, 483, 723, 810, 933
BstNSI	RCATGY	2 cut(s)	943, 2060
BstPAI	GACNNNNGTC	1 cut(s)	1553
BstSCI	CCNGG	9 cut(s)	226, 481, 721, 808, 856, 857, 931, 1250, 2404
BstSFI	CTRYAG	2 cut(s)	1785, 1890
BstUI	CGCG	1 cut(s)	986
BstV1I	GCAGC	4 cut(s)	229, 767, 1129, 1771
BstV2I	GAAGAC	2 cut(s)	55, 1727
BstX2I	RGATCY	1 cut(s)	1254
BstYI	RGATCY	1 cut(s)	1254
Bsu15I	ATCGAT	1 cut(s)	204
BsuI	GTATCC	2 cut(s)	368, 774
BsuRI	GGCC	5 cut(s)	721, 733, 1380, 1853, 2091
BsuTUI	ATCGAT	1 cut(s)	204
BtgZI	GCGATG	1 cut(s)	2463
BtrI	CACGTC	1 cut(s)	1033
BtsCI	GGATG	7 cut(s)	180, 334, 796, 1042, 1359, 1433, 2145
BtsI	GCAGTG	1 cut(s)	765
BtsIMutI	CAGTG	6 cut(s)	33, 429, 765, 921, 1051, 1887
BtuMI	TCGCGA	1 cut(s)	986
Cac8I	GCNNGC	5 cut(s)	941, 945, 971, 1851, 2058
CaiI	CAGNNNCTG	3 cut(s)	722, 1784, 2393
CciI	TCATGA	2 cut(s)	627, 1545
CfoI	GCGC	2 cut(s)	23, 2245
Cfr10I	RCCGGY	1 cut(s)	1805
Cfr9I	CCCGGG	1 cut(s)	857
ClaI	ATCGAT	1 cut(s)	204
CpoI	CGGWCCG	1 cut(s)	2348
CseI	GACGC	2 cut(s)	1033, 2109
Csp6I	GTAC	4 cut(s)	929, 1124, 1955, 2422
CspI	CGGWCCG	1 cut(s)	2348
CviQI	GTAC	4 cut(s)	929, 1124, 1955, 2422
DdeI	CTNAG	2 cut(s)	1342, 2435
DpnI	GATC	8 cut(s)	203, 702, 1256, 1407, 1628, 1842, 1865, 2520
DpnII	GATC	8 cut(s)	201, 700, 1254, 1405, 1626, 1840, 1863, 2518
DraI	TTTAAA	1 cut(s)	1228
DraIII	CACNNNGTG	1 cut(s)	2408
EaeI	YGGCCR	2 cut(s)	731, 1851
Eam1104I	CTCTTC	6 cut(s)	56, 192, 309, 587, 591, 1105
EarI	CTCTTC	6 cut(s)	56, 192, 309, 587, 591, 1105
Ecl136II	GAGCTC	1 cut(s)	2296
Eco130I	CCWWGG	1 cut(s)	1187
Eco24I	GRGCYC	2 cut(s)	617, 2298
Eco47I	GGWCC	8 cut(s)	290, 556, 1183, 1820, 2348, 2364, 2411, 2571
Eco53kI	GAGCTC	1 cut(s)	2296
Eco57I	CTGAAG	4 cut(s)	180, 216, 615, 2136
Eco72I	CACGTG	1 cut(s)	950
Eco88I	CYCGRG	1 cut(s)	857
EcoICRI	GAGCTC	1 cut(s)	2296
EcoO109I	RGGNCCY	2 cut(s)	556, 719
EcoRI	GAATTC	1 cut(s)	418
EcoRII	CCWGG	5 cut(s)	226, 481, 721, 808, 931
EcoT14I	CCWWGG	1 cut(s)	1187
EcoT22I	ATGCAT	1 cut(s)	439
EcoT38I	GRGCYC	2 cut(s)	617, 2298
ErhI	CCWWGG	1 cut(s)	1187
Esp3I	CGTCTC	1 cut(s)	2127
FalI	AAGNNNNNCTT	4 cut(s)	1102, 1134, 2447, 2479
FaqI	GGGAC	3 cut(s)	542, 700, 1259
FauI	CCCGC	1 cut(s)	567
FbaI	TGATCA	1 cut(s)	2518
FblI	GTMKAC	1 cut(s)	744
Fnu4HI	GCNGC	4 cut(s)	243, 756, 1143, 1785
FokI	GGATG	7 cut(s)	167, 341, 803, 1049, 1346, 1420, 2152
FriOI	GRGCYC	2 cut(s)	617, 2298
Fsp4HI	GCNGC	4 cut(s)	243, 756, 1143, 1785
FspBI	CTAG	9 cut(s)	165, 353, 863, 996, 1011, 1188, 1577, 1766, 2592
GlaI	GCGC	2 cut(s)	22, 2244
GluI	GCNGC	4 cut(s)	243, 756, 1143, 1785
GsaI	CCCAGC	1 cut(s)	614
GsuI	CTGGAG	4 cut(s)	504, 831, 2312, 2370
HaeIII	GGCC	5 cut(s)	721, 733, 1380, 1853, 2091
HapII	CCGG	7 cut(s)	131, 858, 1251, 1806, 2063, 2346, 2405
HgaI	GACGC	2 cut(s)	1033, 2109
HhaI	GCGC	2 cut(s)	23, 2245
Hin6I	GCGC	2 cut(s)	21, 2243
HinP1I	GCGC	2 cut(s)	21, 2243
HincII	GTYRAC	1 cut(s)	475
HindII	GTYRAC	1 cut(s)	475
HindIII	AAGCTT	1 cut(s)	2133
HinfI	GANTC	7 cut(s)	39, 153, 319, 545, 1542, 1797, 2027
HpaI	GTTAAC	1 cut(s)	475
HpaII	CCGG	7 cut(s)	131, 858, 1251, 1806, 2063, 2346, 2405
HphI	GGTGA	3 cut(s)	286, 503, 1795
Hpy166II	GTNNAC	4 cut(s)	94, 475, 745, 919
Hpy188I	TCNGA	6 cut(s)	160, 1298, 1405, 1462, 2575, 2618
Hpy8I	GTNNAC	4 cut(s)	94, 475, 745, 919
Hpy99I	CGWCG	2 cut(s)	176, 1864
HpyAV	CCTTC	7 cut(s)	212, 271, 916, 991, 1679, 2103, 2545
HpyCH4III	ACNGT	7 cut(s)	467, 916, 1046, 1891, 1917, 2332, 2352
HpyCH4IV	ACGT	5 cut(s)	248, 672, 949, 1032, 2524
HpyF10VI	GCNNNNNNNGC	7 cut(s)	607, 621, 1511, 1775, 1784, 2141, 2251
HpyF3I	CTNAG	2 cut(s)	1342, 2435
HpySE526I	ACGT	5 cut(s)	248, 672, 949, 1032, 2524
HspAI	GCGC	2 cut(s)	21, 2243
KflI	GGGWCCC	1 cut(s)	556
Kpn2I	TCCGGA	1 cut(s)	2062
Ksp22I	TGATCA	1 cut(s)	2518
KspAI	GTTAAC	1 cut(s)	475
Kzo9I	GATC	8 cut(s)	201, 700, 1254, 1405, 1626, 1840, 1863, 2518
LguI	GCTCTTC	1 cut(s)	591
LmnI	GCTCC	7 cut(s)	145, 485, 659, 1481, 1736, 2065, 2293
Lsp1109I	GCAGC	4 cut(s)	229, 767, 1129, 1771
LweI	GCATC	5 cut(s)	424, 446, 521, 1027, 1927
MaeI	CTAG	9 cut(s)	165, 353, 863, 996, 1011, 1188, 1577, 1766, 2592
MaeII	ACGT	5 cut(s)	248, 672, 949, 1032, 2524
MaeIII	GTNAC	5 cut(s)	31, 887, 1046, 1801, 2326
MalI	GATC	8 cut(s)	203, 702, 1256, 1407, 1628, 1842, 1865, 2520
MboI	GATC	8 cut(s)	201, 700, 1254, 1405, 1626, 1840, 1863, 2518
MfeI	CAATTG	1 cut(s)	263
MflI	RGATCY	1 cut(s)	1254
MhlI	GDGCHC	2 cut(s)	617, 2298
MlsI	TGGCCA	2 cut(s)	733, 1853
MluNI	TGGCCA	2 cut(s)	733, 1853
MlyI	GAGTC	1 cut(s)	313
MmeI	TCCRAC	2 cut(s)	1984, 2292
Mox20I	TGGCCA	2 cut(s)	733, 1853
Mph1103I	ATGCAT	1 cut(s)	439
MroI	TCCGGA	1 cut(s)	2062
MscI	TGGCCA	2 cut(s)	733, 1853
MslI	CAYNNNNRTG	2 cut(s)	183, 1568
Msp20I	TGGCCA	2 cut(s)	733, 1853
MspA1I	CMGCKG	1 cut(s)	1142
MspI	CCGG	7 cut(s)	131, 858, 1251, 1806, 2063, 2346, 2405
MspR9I	CCNGG	9 cut(s)	228, 483, 723, 810, 858, 859, 933, 1252, 2406
MunI	CAATTG	1 cut(s)	263
Mva1269I	GAATGC	1 cut(s)	457
MvaI	CCWGG	5 cut(s)	228, 483, 723, 810, 933
MvnI	CGCG	1 cut(s)	986
MwoI	GCNNNNNNNGC	7 cut(s)	607, 621, 1511, 1775, 1784, 2141, 2251
NciI	CCSGG	4 cut(s)	858, 859, 1252, 2406
NdeII	GATC	8 cut(s)	201, 700, 1254, 1405, 1626, 1840, 1863, 2518
NlaIV	GGNNCC	6 cut(s)	334, 557, 558, 1185, 1821, 2186
NmuCI	GTSAC	4 cut(s)	31, 1046, 1801, 2326
NruI	TCGCGA	1 cut(s)	986
NsiI	ATGCAT	1 cut(s)	439
NspI	RCATGY	2 cut(s)	943, 2060
OliI	CACNNNNGTG	1 cut(s)	183
PaeI	GCATGC	2 cut(s)	943, 2060
PagI	TCATGA	2 cut(s)	627, 1545
PciSI	GCTCTTC	1 cut(s)	591
PcsI	WCGNNNNNNNCGW	1 cut(s)	144
PctI	GAATGC	1 cut(s)	457
PfeI	GAWTC	6 cut(s)	39, 153, 545, 1542, 1797, 2027
PflMI	CCANNNNNTGG	3 cut(s)	1323, 1754, 2408
PfoI	TCCNGGA	2 cut(s)	481, 808
PkrI	GCNGC	4 cut(s)	244, 757, 1144, 1786
PleI	GAGTC	1 cut(s)	313
PmaCI	CACGTG	1 cut(s)	950
PmlI	CACGTG	1 cut(s)	950
PpsI	GAGTC	1 cut(s)	313
Ppu21I	YACGTR	1 cut(s)	950
PpuMI	RGGWCCY	1 cut(s)	556
PshAI	GACNNNNGTC	1 cut(s)	1553
PshBI	ATTAAT	1 cut(s)	1712
PsiI	TTATAA	1 cut(s)	668
Psp124BI	GAGCTC	1 cut(s)	2298
Psp5II	RGGWCCY	1 cut(s)	556
Psp6I	CCWGG	5 cut(s)	226, 481, 721, 808, 931
PspCI	CACGTG	1 cut(s)	950
PspFI	CCCAGC	1 cut(s)	610
PspGI	CCWGG	5 cut(s)	226, 481, 721, 808, 931
PspN4I	GGNNCC	6 cut(s)	334, 557, 558, 1185, 1821, 2186
PspPPI	RGGWCCY	1 cut(s)	556
PstI	CTGCAG	1 cut(s)	1789
PstNI	CAGNNNCTG	3 cut(s)	722, 1784, 2393
PsuI	RGATCY	1 cut(s)	1254
PvuII	CAGCTG	1 cut(s)	1142
RruI	TCGCGA	1 cut(s)	986
RsaI	GTAC	4 cut(s)	930, 1125, 1956, 2423
RsaNI	GTAC	4 cut(s)	929, 1124, 1955, 2422
RseI	CAYNNNNRTG	2 cut(s)	183, 1568
Rsr2I	CGGWCCG	1 cut(s)	2348
RsrII	CGGWCCG	1 cut(s)	2348
SacI	GAGCTC	1 cut(s)	2298
SapI	GCTCTTC	1 cut(s)	591
SatI	GCNGC	4 cut(s)	243, 756, 1143, 1785
Sau3AI	GATC	8 cut(s)	201, 700, 1254, 1405, 1626, 1840, 1863, 2518
SchI	GAGTC	1 cut(s)	313
ScrFI	CCNGG	9 cut(s)	228, 483, 723, 810, 858, 859, 933, 1252, 2406
SduI	GDGCHC	2 cut(s)	617, 2298
SfaNI	GCATC	5 cut(s)	424, 446, 521, 1027, 1927
SfcI	CTRYAG	2 cut(s)	1785, 1890
SinI	GGWCC	8 cut(s)	290, 556, 1183, 1820, 2348, 2364, 2411, 2571
SmaI	CCCGGG	1 cut(s)	859
SmiMI	CAYNNNNRTG	2 cut(s)	183, 1568
SmlI	CTYRAG	4 cut(s)	321, 750, 2002, 2127
SmoI	CTYRAG	4 cut(s)	321, 750, 2002, 2127
SpeI	ACTAGT	1 cut(s)	2591
SphI	GCATGC	2 cut(s)	943, 2060
SsiI	CCGC	5 cut(s)	125, 560, 885, 1904, 2252
SspI	AATATT	1 cut(s)	2206
SspMI	CTAG	9 cut(s)	165, 353, 863, 996, 1011, 1188, 1577, 1766, 2592
SstI	GAGCTC	1 cut(s)	2298
StyD4I	CCNGG	9 cut(s)	226, 481, 721, 808, 856, 857, 931, 1250, 2404
StyI	CCWWGG	1 cut(s)	1187
TaaI	ACNGT	7 cut(s)	467, 916, 1046, 1891, 1917, 2332, 2352
TaiI	ACGT	5 cut(s)	251, 675, 952, 1035, 2527
TaqI	TCGA	6 cut(s)	147, 174, 204, 909, 1625, 1862
TatI	WGTACW	1 cut(s)	1954
TfiI	GAWTC	6 cut(s)	39, 153, 545, 1542, 1797, 2027
TscAI	CASTG	6 cut(s)	40, 436, 765, 921, 1051, 1894
TseFI	GTSAC	4 cut(s)	31, 1046, 1801, 2326
TseI	GCWGC	4 cut(s)	242, 755, 1142, 1784
Tsp45I	GTSAC	4 cut(s)	31, 1046, 1801, 2326
TspGWI	ACGGA	2 cut(s)	88, 173
TspMI	CCCGGG	1 cut(s)	857
TspRI	CASTG	6 cut(s)	40, 436, 765, 921, 1051, 1894
Van91I	CCANNNNNTGG	3 cut(s)	1323, 1754, 2408
VpaK11BI	GGWCC	8 cut(s)	290, 556, 1183, 1820, 2348, 2364, 2411, 2571
VspI	ATTAAT	1 cut(s)	1712
XapI	RAATTY	6 cut(s)	44, 113, 295, 418, 1871, 2032
XceI	RCATGY	2 cut(s)	943, 2060
XcmI	CCANNNNNNNNNTGG	2 cut(s)	719, 1048
XmaI	CCCGGG	1 cut(s)	857
XmaJI	CCTAGG	1 cut(s)	1187
XmiI	GTMKAC	1 cut(s)	744
XspI	CTAG	9 cut(s)	165, 353, 863, 996, 1011, 1188, 1577, 1766, 2592
Zsp2I	ATGCAT	1 cut(s)	439

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.