Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000005

Orthogroup ID

OG0005119

Physical Location & Seq

Genomic Coordinates Forward (+)

53770229 .. 53777676

Gene Structure 7448 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_5G00372320.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2409 bp

ATGGCGCCGAGGCTCGGCTCCGGCGACGTGTCCGGCTTCAAGTTCCTCCTCTTTCTCGTCGCGATTTACGGCCTCATCGCCGTCCTGGTGCACTCGATTCTCCACATGCGCTTCATTACGCCGCTTGGGATCGACGCGCCGCTCGATCGATTCTCCGAGGCCCGAGCCGTCGAGCACGTTCGAGTCCTGGCCAAAGATATCGATAGCCGCCAGGAAGGGAGTCCTGGATTGGGAGAAGCTGCTCGATATATCATAGCGCAGTTGGAGATGGTAAAGGAGCGAGCTGGACCTAACTTTAGAGTTGAGATAGAAGAGACTGTTGTGAATGGCACGTTCAATATGCTGTTTTTAGGGTATAGCATATCTCTGGGGTACAGAAACCACACAAATATTGTGATGAGAATATCATCAGTAGATTCACAAGACTCTGATCCGTCAGTATTGTTGAATGGCCATTTTGATAGTCCACTTGGTTCTCCGGGTGCTGGTGATTGTGGGTCATGTGTAGCATCAATGCTGGAAATAGCAAGACTTATTGTGGACTCTGGATGGGTTCCCCCTCAGCCTGTTATTTTTCTTTTTAATGGTGCAGAAGAGCTCTTTTTGCTGGGGTCTCATGGCTTCATGAAGACACATAAATGGCGTGAAACTATTGGGGCTTTTATAAATGTGGAAGCGTCGGGGACAGGCGGTCCAGATTTAGTTTGCCAATCAGGACCTAGTTCCTGGCCTTCTCAGGTCTATGCTCAATCAGCAGTATATCCCATGGCACATAGCGCAGCCCAGGATGTTTTTCCTGTTGTTCCTGGAGATACAGATTTTCGAATATTTTCCGAGGACTATGGTGACATTCCTGGCTTGGATATTATCTTTCTCCTTGGTGGTTATTTTTATCATACATCCTTCGATACAGTGGAGAGACTATTACCTGGAAGTATACAAGCTCGTGGAGAAAATCTGTTTAGCATACTTAAAGCCTTCACCAATTCTTCTACGCTACAAAATACTCTCGAAAGACATTCTAATGTATCAACTGCCAAGCAGCATGAGGATGGACGTGCTGTTTTCTTTGATTATTTAACATGGTTCATGGTAGCTATGGTACTTCACCCTATTCCTATTGGCATCTTCCTGGCTATGCCATTTTTCTCACAAAAGCAGAATCCTGGGCTCCTACCTTGGTTTGCTACTTTCAGCAATTTTGTAAAAGGAATGCTTTTCCATGCTGCTGGGGTTTTTCTAGCAATTGTAATTCCGGTTATATTTTCCATCGTGAGATTGTTGTTTGCTAGTCGTGCAATGAACTGGTTTGCTCATCCATATTTGGCTTATTTGATGTTCATACCTTGCTCTCTTGTTGGTCTGTTAATTCCAAGATTTGTTTGGAGTAGCTTTCCCCTCACTCAAGAGGCATCAGTTGTCAAGTCTTTAAAAGAGGCTTTATCGGATGAAGCAAGGTTCTGGGGAGCTTTTGGATTATACGCTACAATAACTGTGTTTTTGGTTGAAAAGATGGGTATGATGGGTGCTTTGCCACCTCCATATGGTTATTTCGTTCCTGACATTGTGGTGGCTGCAGTAATTGGAGTTGTGACCAGTTGGTCTGTAGGCCCTCTGATACCTATTTGTGGTCACTGGTTAGCCAGGTCATCCATTTTGCAAGTCTTGTTGCATCTTAGTGTGCTAGGCTTGGCTCTGTCATCTCAGTTCTTTCCATATAGCACGGGTGCACCTAAGAGAGTTGTTTTCCAGCATACTTTTCTTACCGCAGATGCAAATCATGTTGTGGAGTCCAGCTATGATTTTTCTGTTGTGGATTCCAATTCGTTACTTTTTCTTTTCAAACATGCACCTGAGGTGGCAAAGGAATTGCACATTAGTTCAGAGTTGTCTTTTGAAACTGCAAAGTCATCTCACCGAGAGACTTGGATGGGACTTTTTCCTGTTTCTTTTTTGTTTTCAAGAAGCTTGAAGTTCCCCGCAAGAAGTGATGGTATTTTAAAGCAATACGGGCAGTTCCCTCATCTGTCCACTTATAAGCCACATACAGTATTTGGCGAGGAATCTCGTAGAATATACTTGGAACTGTCTTTAGGTTCCTTAGAAGAGGTTTGGGTTGCAGTCCTCAATATTACAGGCCCTTTATCTAGTTGGTCATTTGCAGACAATACACTTTCAGCTACTGAAACATCTGATGGAGGTCCACCATCATACATATGCCGACTTAGTGGAGCAAGTCCTGAGAATTGGACCTTCTGGTTAGAGGCTAACAGGTCTGAAGATCTAAGGGTAGAAGTTGCTGTCGTAGATCAATATATGGTTGATGAAGCAAAGAAGCTGAAAGGGCTTTTTCCGGAGTGGGTGGATGTAGTTGCTTATTCGGGCTTTTTGTCGAGTTATATTTTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001541 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0007275 GO:0007548 GO:0008150 GO:0008406 GO:0008585 GO:0012505 GO:0022414 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0045137 GO:0046545 GO:0046660 GO:0048513 GO:0048608 GO:0048731 GO:0048856 GO:0061458

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_M28

Protein Analysis

802

Amino Acids

88.63

Weight (kDa)

5.59

Isoelectric Point (pI)

35.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M28	PF04389	130 - 321	4.9e-44	Peptidase family M28
ERMP1_C	PF22248	577 - 800	4e-31	Endoplasmic reticulum metallopeptidase 1, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005119)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G67420 AT1G67420 AT1G67420 AT1G67420 AT1G67420 AT1G67420
fragaria_vesca	FvH4_4g23910 FvH4_4g23910 FvH4_4g23910 FvH4_4g23910 FvH4_4g23910 FvH4_4g23910
malus_domestica	MD13G1117700.v1.1 MD16G1117900.v1.1
prunus_persica	Prupe.1G227300_v2.0.a1 Prupe.1G227300_v2.0.a1 Prupe.1G227300_v2.0.a1
pyrus_communis	pycom13g10180 pycom16g10100
rosa_chinensis	RchiOBHm_Chr4g0430551
rosa_laevigata	RLG00000006970
rosa_multiflora	Rmu_sc0011782.1_g000005
rosa_roxburghii	Rroxscaffold_5G00372320
rosa_rugosa	Rorug04G0244100 Rorug04G0244200
rosa_samantha	Rh4AG300200 Rh4BG307300 Rh4CG324000 Rh4DG304600
rosa_wichuraiana	Rw4G026050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	665, 2037
AasI	GACNNNNNNGTC	1 cut(s)	1600
AccB1I	GGYRCC	1 cut(s)	4
AccBSI	CCGCTC	1 cut(s)	142
AccI	GTMKAC	1 cut(s)	937
AccII	CGCG	2 cut(s)	62, 137
AccIII	TCCGGA	1 cut(s)	2353
AciI	CCGC	6 cut(s)	122, 140, 208, 690, 1767, 1980
AclWI	GGATC	2 cut(s)	137, 425
AcoI	YGGCCR	2 cut(s)	189, 451
AcuI	CTGAAG	1 cut(s)	2298
AcyI	GRCGYC	1 cut(s)	5
AfaI	GTAC	2 cut(s)	374, 1104
AfiI	CCNNNNNNNGG	5 cut(s)	14, 230, 485, 1544, 1628
AflIII	ACRYGT	1 cut(s)	27
AgsI	TTSAA	8 cut(s)	40, 337, 448, 1508, 1843, 1898, 1962, 1972
AhdI	GACNNNNNGTC	1 cut(s)	690
AjiI	CACGTC	2 cut(s)	28, 1058
Alw21I	GWGCWC	4 cut(s)	93, 177, 600, 1732
Alw26I	GTCTC	4 cut(s)	308, 618, 913, 1916
Alw44I	GTGCAC	2 cut(s)	89, 1728
AlwI	GGATC	2 cut(s)	137, 425
AlwNI	CAGNNNCTG	1 cut(s)	2183
Ama87I	CYCGRG	1 cut(s)	162
Aor13HI	TCCGGA	1 cut(s)	2353
AoxI	GGCC	7 cut(s)	70, 159, 189, 451, 728, 1609, 2137
ApaLI	GTGCAC	2 cut(s)	89, 1728
ApeKI	GCWGC	5 cut(s)	239, 779, 1042, 1226, 1574
Asp700I	GAANNNNTTC	1 cut(s)	829
AspLEI	GCGC	5 cut(s)	7, 111, 139, 259, 779
AspS9I	GGNCC	8 cut(s)	160, 287, 692, 716, 1610, 2138, 2201, 2250
AsuC2I	CCSGG	1 cut(s)	480
AsuHPI	GGTGA	5 cut(s)	500, 857, 973, 1100, 1907
AsuII	TTCGAA	1 cut(s)	823
AvaI	CYCGRG	1 cut(s)	162
AvaII	GGWCC	5 cut(s)	287, 692, 716, 2201, 2250
AxyI	CCTNAGG	1 cut(s)	1854
BaeGI	GKGCMC	2 cut(s)	93, 1732
BalI	TGGCCA	2 cut(s)	191, 453
BanI	GGYRCC	1 cut(s)	4
BanII	GRGCYC	2 cut(s)	600, 1173
BauI	CACGAG	1 cut(s)	945
BbsI	GAAGAC	1 cut(s)	635
Bbv12I	GWGCWC	4 cut(s)	93, 177, 600, 1732
BbvCI	CCTCAGC	1 cut(s)	561
BbvI	GCAGC	5 cut(s)	226, 791, 1054, 1213, 1561
BceAI	ACGGC	3 cut(s)	65, 85, 152
BcnI	CCSGG	1 cut(s)	480
BcoDI	GTCTC	4 cut(s)	308, 618, 913, 1916
BfaI	CTAG	6 cut(s)	720, 1241, 1290, 1685, 2148, 2407
BfmI	CTRYAG	2 cut(s)	1575, 1605
BfoI	RGCGCY	1 cut(s)	8
BglII	AGATCT	1 cut(s)	2281
BisI	GCNGC	8 cut(s)	122, 140, 208, 240, 780, 1043, 1227, 1575
BlsI	GCNGC	8 cut(s)	123, 141, 209, 241, 781, 1044, 1228, 1576
Bme18I	GGWCC	5 cut(s)	287, 692, 716, 2201, 2250
BmeRI	GACNNNNNGTC	1 cut(s)	690
BmeT110I	CYCGRG	1 cut(s)	162
BmgBI	CACGTC	2 cut(s)	28, 1058
BmgT120I	GGNCC	8 cut(s)	160, 287, 692, 716, 1610, 2138, 2201, 2250
BmiI	GGNNCC	5 cut(s)	6, 19, 555, 1172, 2098
BmsI	GCATC	5 cut(s)	518, 1134, 1421, 1681, 1762
BpiI	GAAGAC	1 cut(s)	635
BpmI	CTGGAG	1 cut(s)	828
Bpu10I	CCTNAGC	1 cut(s)	561
Bpu14I	TTCGAA	1 cut(s)	823
BpuEI	CTTGAG	1 cut(s)	1389
BpuMI	CCSGG	1 cut(s)	480
Bsa29I	ATCGAT	2 cut(s)	148, 201
BsaBI	GATNNNNATC	1 cut(s)	1776
BsaHI	GRCGYC	1 cut(s)	5
BsaI	GGTCTC	1 cut(s)	618
BsaJI	CCNNGG	8 cut(s)	8, 156, 765, 783, 834, 877, 1166, 1178
BsaWI	WCCGGW	2 cut(s)	1255, 2353
Bsc4I	CCNNNNNNNGG	5 cut(s)	14, 230, 485, 1544, 1628
Bse1I	ACTGG	3 cut(s)	1310, 1596, 1640
Bse21I	CCTNAGG	1 cut(s)	1854
Bse3DI	GCAATG	1 cut(s)	1305
Bse8I	GATNNNNATC	1 cut(s)	1776
BseAI	TCCGGA	1 cut(s)	2353
BseCI	ATCGAT	2 cut(s)	148, 201
BseDI	CCNNGG	8 cut(s)	8, 156, 765, 783, 834, 877, 1166, 1178
BseGI	GGATG	9 cut(s)	554, 793, 899, 1057, 1315, 1453, 1649, 1935, 2371
BseJI	GATNNNNATC	1 cut(s)	1776
BseLI	CCNNNNNNNGG	5 cut(s)	14, 230, 485, 1544, 1628
BseMI	GCAATG	1 cut(s)	1305
BseMII	CTCAG	5 cut(s)	575, 749, 1718, 1845, 2232
BseNI	ACTGG	3 cut(s)	1310, 1596, 1640
BseRI	GAGGAG	1 cut(s)	38
BseSI	GKGCMC	2 cut(s)	93, 1732
BseXI	GCAGC	5 cut(s)	226, 791, 1054, 1213, 1561
BseYI	CCCAGC	2 cut(s)	607, 1229
BsgI	GTGCAG	1 cut(s)	609
Bsh1236I	CGCG	2 cut(s)	62, 137
Bsh1285I	CGRYCG	1 cut(s)	148
BshFI	GGCC	7 cut(s)	72, 161, 191, 453, 730, 1611, 2139
BshNI	GGYRCC	1 cut(s)	4
BshVI	ATCGAT	2 cut(s)	148, 201
BsiEI	CGRYCG	1 cut(s)	148
BsiHKAI	GWGCWC	4 cut(s)	93, 177, 600, 1732
BsiHKCI	CYCGRG	1 cut(s)	162
BsiSI	CCGG	5 cut(s)	21, 33, 479, 1256, 2354
BslFI	GGGAC	2 cut(s)	697, 1947
BslI	CCNNNNNNNGG	5 cut(s)	14, 230, 485, 1544, 1628
BsmAI	GTCTC	4 cut(s)	308, 618, 913, 1916
BsmFI	GGGAC	2 cut(s)	697, 1947
BsmI	GAATGC	1 cut(s)	1218
BsnI	GGCC	7 cut(s)	72, 161, 191, 453, 730, 1611, 2139
Bso31I	GGTCTC	1 cut(s)	618
BsoBI	CYCGRG	1 cut(s)	162
Bsp119I	TTCGAA	1 cut(s)	823
Bsp1286I	GDGCHC	5 cut(s)	93, 177, 600, 1173, 1732
Bsp13I	TCCGGA	1 cut(s)	2353
Bsp143I	GATC	5 cut(s)	129, 145, 430, 2281, 2308
Bsp19I	CCATGG	1 cut(s)	765
Bsp68I	TCGCGA	1 cut(s)	62
BspACI	CCGC	6 cut(s)	122, 140, 208, 690, 1767, 1980
BspANI	GGCC	7 cut(s)	72, 161, 191, 453, 730, 1611, 2139
BspCNI	CTCAG	5 cut(s)	574, 748, 1717, 1846, 2233
BspDI	ATCGAT	2 cut(s)	148, 201
BspEI	TCCGGA	1 cut(s)	2353
BspFNI	CGCG	2 cut(s)	62, 137
BspHI	TCATGA	1 cut(s)	624
BspLI	GGNNCC	5 cut(s)	6, 19, 555, 1172, 2098
BspMAI	CTGCAG	1 cut(s)	1579
BspPI	GGATC	2 cut(s)	137, 425
BspQI	GCTCTTC	1 cut(s)	588
BspT104I	TTCGAA	1 cut(s)	823
BspT107I	GGYRCC	1 cut(s)	4
BspTNI	GGTCTC	1 cut(s)	618
BsrBI	CCGCTC	1 cut(s)	142
BsrDI	GCAATG	1 cut(s)	1305
BsrI	ACTGG	3 cut(s)	1310, 1596, 1640
BssECI	CCNNGG	8 cut(s)	8, 156, 765, 783, 834, 877, 1166, 1178
BssMI	GATC	5 cut(s)	129, 145, 430, 2281, 2308
BssNAI	GTATAC	1 cut(s)	938
BssNI	GRCGYC	1 cut(s)	5
BssSI	CACGAG	1 cut(s)	945
BssT1I	CCWWGG	3 cut(s)	765, 877, 1178
Bst1107I	GTATAC	1 cut(s)	938
Bst2BI	CACGAG	1 cut(s)	945
Bst4CI	ACNGT	5 cut(s)	319, 913, 1495, 2050, 2088
Bst6I	CTCTTC	3 cut(s)	306, 588, 2100
BstACI	GRCGYC	1 cut(s)	5
BstBI	TTCGAA	1 cut(s)	823
BstC8I	GCNNGC	1 cut(s)	282
BstDSI	CCRYGG	1 cut(s)	765
BstF5I	GGATG	9 cut(s)	554, 793, 899, 1057, 1315, 1453, 1649, 1935, 2371
BstFNI	CGCG	2 cut(s)	62, 137
BstH2I	RGCGCY	1 cut(s)	8
BstHHI	GCGC	5 cut(s)	7, 111, 139, 259, 779
BstKTI	GATC	5 cut(s)	132, 148, 433, 2284, 2311
BstMAI	GTCTC	4 cut(s)	308, 618, 913, 1916
BstMBI	GATC	5 cut(s)	129, 145, 430, 2281, 2308
BstMCI	CGRYCG	1 cut(s)	148
BstMWI	GCNNNNNNNGC	6 cut(s)	604, 752, 776, 1295, 2011, 2344
BstNSI	RCATGY	2 cut(s)	109, 1850
BstSFI	CTRYAG	2 cut(s)	1575, 1605
BstSLI	GKGCMC	2 cut(s)	93, 1732
BstUI	CGCG	2 cut(s)	62, 137
BstV1I	GCAGC	5 cut(s)	226, 791, 1054, 1213, 1561
BstV2I	GAAGAC	1 cut(s)	635
BstX2I	RGATCY	1 cut(s)	2281
BstXI	CCANNNNNNTGG	1 cut(s)	1229
BstYI	RGATCY	1 cut(s)	2281
BstZ17I	GTATAC	1 cut(s)	938
Bsu15I	ATCGAT	2 cut(s)	148, 201
Bsu36I	CCTNAGG	1 cut(s)	1854
BsuRI	GGCC	7 cut(s)	72, 161, 191, 453, 730, 1611, 2139
BsuTUI	ATCGAT	2 cut(s)	148, 201
BtgI	CCRYGG	1 cut(s)	765
BtgZI	GCGATG	1 cut(s)	61
BtrI	CACGTC	2 cut(s)	28, 1058
BtsCI	GGATG	9 cut(s)	554, 793, 899, 1057, 1315, 1453, 1649, 1935, 2371
BtsIMutI	CAGTG	2 cut(s)	918, 1633
BtuMI	TCGCGA	1 cut(s)	62
Cac8I	GCNNGC	1 cut(s)	282
CaiI	CAGNNNCTG	1 cut(s)	2183
CciI	TCATGA	1 cut(s)	624
CfoI	GCGC	5 cut(s)	7, 111, 139, 259, 779
Cfr13I	GGNCC	8 cut(s)	160, 287, 692, 716, 1610, 2138, 2201, 2250
ClaI	ATCGAT	2 cut(s)	148, 201
CseI	GACGC	2 cut(s)	143, 666
Csp6I	GTAC	2 cut(s)	373, 1103
CviQI	GTAC	2 cut(s)	373, 1103
DinI	GGCGCC	1 cut(s)	6
DpnI	GATC	5 cut(s)	131, 147, 432, 2283, 2310
DpnII	GATC	5 cut(s)	129, 145, 430, 2281, 2308
DraI	TTTAAA	2 cut(s)	1431, 2001
DrdI	GACNNNNNNGTC	1 cut(s)	1600
DriI	GACNNNNNGTC	1 cut(s)	690
DseDI	GACNNNNNNGTC	1 cut(s)	1600
EaeI	YGGCCR	2 cut(s)	189, 451
Eam1104I	CTCTTC	3 cut(s)	306, 588, 2100
Eam1105I	GACNNNNNGTC	1 cut(s)	690
EarI	CTCTTC	3 cut(s)	306, 588, 2100
Ecl136II	GAGCTC	1 cut(s)	598
Eco130I	CCWWGG	3 cut(s)	765, 877, 1178
Eco24I	GRGCYC	2 cut(s)	600, 1173
Eco31I	GGTCTC	1 cut(s)	618
Eco32I	GATATC	1 cut(s)	199
Eco47I	GGWCC	5 cut(s)	287, 692, 716, 2201, 2250
Eco53kI	GAGCTC	1 cut(s)	598
Eco57I	CTGAAG	1 cut(s)	2298
Eco81I	CCTNAGG	1 cut(s)	1854
Eco88I	CYCGRG	1 cut(s)	162
EcoICRI	GAGCTC	1 cut(s)	598
EcoO109I	RGGNCCY	3 cut(s)	716, 1610, 2138
EcoRV	GATATC	1 cut(s)	199
EcoT14I	CCWWGG	3 cut(s)	765, 877, 1178
EcoT38I	GRGCYC	2 cut(s)	600, 1173
EgeI	GGCGCC	1 cut(s)	6
EheI	GGCGCC	1 cut(s)	6
ErhI	CCWWGG	3 cut(s)	765, 877, 1178
FalI	AAGNNNNNCTT	2 cut(s)	1200, 1232
FaqI	GGGAC	2 cut(s)	697, 1947
FauI	CCCGC	1 cut(s)	1987
FauNDI	CATATG	2 cut(s)	1543, 2216
FblI	GTMKAC	1 cut(s)	937
Fnu4HI	GCNGC	8 cut(s)	122, 140, 208, 240, 780, 1043, 1227, 1575
FokI	GGATG	9 cut(s)	561, 800, 886, 1064, 1302, 1460, 1636, 1942, 2378
FriOI	GRGCYC	2 cut(s)	600, 1173
Fsp4HI	GCNGC	8 cut(s)	122, 140, 208, 240, 780, 1043, 1227, 1575
FspBI	CTAG	6 cut(s)	720, 1241, 1290, 1685, 2148, 2407
GlaI	GCGC	5 cut(s)	6, 110, 138, 258, 778
GluI	GCNGC	8 cut(s)	122, 140, 208, 240, 780, 1043, 1227, 1575
GsaI	CCCAGC	2 cut(s)	611, 1233
GsuI	CTGGAG	1 cut(s)	828
HaeII	RGCGCY	1 cut(s)	8
HaeIII	GGCC	7 cut(s)	72, 161, 191, 453, 730, 1611, 2139
HapII	CCGG	5 cut(s)	21, 33, 479, 1256, 2354
HgaI	GACGC	2 cut(s)	143, 666
HhaI	GCGC	5 cut(s)	7, 111, 139, 259, 779
Hin1I	GRCGYC	1 cut(s)	5
Hin6I	GCGC	5 cut(s)	5, 109, 137, 257, 777
HinP1I	GCGC	5 cut(s)	5, 109, 137, 257, 777
HindIII	AAGCTT	1 cut(s)	1966
HpaII	CCGG	5 cut(s)	21, 33, 479, 1256, 2354
HphI	GGTGA	5 cut(s)	500, 857, 973, 1100, 1907
Hpy166II	GTNNAC	7 cut(s)	91, 467, 541, 938, 1730, 2031, 2204
Hpy188I	TCNGA	8 cut(s)	157, 430, 835, 1447, 1617, 1885, 2194, 2278
Hpy8I	GTNNAC	7 cut(s)	91, 467, 541, 938, 1730, 2031, 2204
Hpy99I	CGWCG	5 cut(s)	29, 62, 137, 173, 682
HpyAV	CCTTC	5 cut(s)	209, 741, 913, 988, 2263
HpyCH4III	ACNGT	5 cut(s)	319, 913, 1495, 2050, 2088
HpyCH4IV	ACGT	4 cut(s)	27, 177, 332, 1057
HpyF10VI	GCNNNNNNNGC	6 cut(s)	604, 752, 776, 1295, 2011, 2344
HpySE526I	ACGT	4 cut(s)	27, 177, 332, 1057
Hsp92I	GRCGYC	1 cut(s)	5
HspAI	GCGC	5 cut(s)	5, 109, 137, 257, 777
KasI	GGCGCC	1 cut(s)	4
Kpn2I	TCCGGA	1 cut(s)	2353
Kzo9I	GATC	5 cut(s)	129, 145, 430, 2281, 2308
LguI	GCTCTTC	1 cut(s)	588
LmnI	GCTCC	5 cut(s)	23, 277, 1176, 1466, 2231
Lsp1109I	GCAGC	5 cut(s)	226, 791, 1054, 1213, 1561
LweI	GCATC	5 cut(s)	518, 1134, 1421, 1681, 1762
MaeI	CTAG	6 cut(s)	720, 1241, 1290, 1685, 2148, 2407
MaeII	ACGT	4 cut(s)	27, 177, 332, 1057
MaeIII	GTNAC	4 cut(s)	845, 1591, 1631, 1827
MalI	GATC	5 cut(s)	131, 147, 432, 2283, 2310
MbiI	CCGCTC	1 cut(s)	142
MboI	GATC	5 cut(s)	129, 145, 430, 2281, 2308
MboII	GAAGA	7 cut(s)	323, 605, 640, 981, 1120, 2117, 2291
MfeI	CAATTG	1 cut(s)	1245
MflI	RGATCY	1 cut(s)	2281
MhlI	GDGCHC	5 cut(s)	93, 177, 600, 1173, 1732
MlsI	TGGCCA	2 cut(s)	191, 453
MluCI	AATT	9 cut(s)	985, 1198, 1245, 1251, 1368, 1581, 1822, 1868, 2245
MluNI	TGGCCA	2 cut(s)	191, 453
Mly113I	GGCGCC	1 cut(s)	5
MlyI	GAGTC	5 cut(s)	192, 229, 419, 536, 1799
MmeI	TCCRAC	1 cut(s)	243
Mox20I	TGGCCA	2 cut(s)	191, 453
MroI	TCCGGA	1 cut(s)	2353
MroXI	GAANNNNTTC	1 cut(s)	829
MscI	TGGCCA	2 cut(s)	191, 453
MseI	TTAA	6 cut(s)	582, 972, 1079, 1367, 1430, 2000
MslI	CAYNNNNRTG	6 cut(s)	637, 1023, 1050, 1568, 1677, 2215
Msp20I	TGGCCA	2 cut(s)	191, 453
MspI	CCGG	5 cut(s)	21, 33, 479, 1256, 2354
MunI	CAATTG	1 cut(s)	1245
Mva1269I	GAATGC	1 cut(s)	1218
MvnI	CGCG	2 cut(s)	62, 137
MwoI	GCNNNNNNNGC	6 cut(s)	604, 752, 776, 1295, 2011, 2344
NarI	GGCGCC	1 cut(s)	5
NciI	CCSGG	1 cut(s)	480
NcoI	CCATGG	1 cut(s)	765
NdeI	CATATG	2 cut(s)	1543, 2216
NdeII	GATC	5 cut(s)	129, 145, 430, 2281, 2308
NlaIV	GGNNCC	5 cut(s)	6, 19, 555, 1172, 2098
NmeAIII	GCCGAG	1 cut(s)	33
NmuCI	GTSAC	3 cut(s)	845, 1591, 1631
NruI	TCGCGA	1 cut(s)	62
NspI	RCATGY	2 cut(s)	109, 1850
NspV	TTCGAA	1 cut(s)	823
PagI	TCATGA	1 cut(s)	624
PciSI	GCTCTTC	1 cut(s)	588
PcsI	WCGNNNNNNNCGW	2 cut(s)	21, 141
PctI	GAATGC	1 cut(s)	1218
PdmI	GAANNNNTTC	1 cut(s)	829
PfeI	GAWTC	6 cut(s)	97, 150, 416, 1162, 1817, 2063
PfoI	TCCNGGA	2 cut(s)	223, 805
PkrI	GCNGC	8 cut(s)	123, 141, 209, 241, 781, 1044, 1228, 1576
Ple19I	CGATCG	1 cut(s)	148
PleI	GAGTC	5 cut(s)	191, 228, 419, 536, 1798
PluTI	GGCGCC	1 cut(s)	8
PpsI	GAGTC	5 cut(s)	191, 228, 419, 536, 1798
PpuMI	RGGWCCY	1 cut(s)	716
PsiI	TTATAA	2 cut(s)	665, 2037
Psp124BI	GAGCTC	1 cut(s)	600
Psp5II	RGGWCCY	1 cut(s)	716
PspFI	CCCAGC	2 cut(s)	607, 1229
PspN4I	GGNNCC	5 cut(s)	6, 19, 555, 1172, 2098
PspPI	GGNCC	8 cut(s)	160, 287, 692, 716, 1610, 2138, 2201, 2250
PspPPI	RGGWCCY	1 cut(s)	716
PstI	CTGCAG	1 cut(s)	1579
PstNI	CAGNNNCTG	1 cut(s)	2183
PsuI	RGATCY	1 cut(s)	2281
PvuI	CGATCG	1 cut(s)	148
RruI	TCGCGA	1 cut(s)	62
RsaI	GTAC	2 cut(s)	374, 1104
RsaNI	GTAC	2 cut(s)	373, 1103
RseI	CAYNNNNRTG	6 cut(s)	637, 1023, 1050, 1568, 1677, 2215
SacI	GAGCTC	1 cut(s)	600
SapI	GCTCTTC	1 cut(s)	588
SaqAI	TTAA	6 cut(s)	582, 972, 1079, 1367, 1430, 2000
SatI	GCNGC	8 cut(s)	122, 140, 208, 240, 780, 1043, 1227, 1575
Sau3AI	GATC	5 cut(s)	129, 145, 430, 2281, 2308
Sau96I	GGNCC	8 cut(s)	160, 287, 692, 716, 1610, 2138, 2201, 2250
SchI	GAGTC	5 cut(s)	192, 229, 419, 536, 1799
SduI	GDGCHC	5 cut(s)	93, 177, 600, 1173, 1732
SfaNI	GCATC	5 cut(s)	518, 1134, 1421, 1681, 1762
SfcI	CTRYAG	2 cut(s)	1575, 1605
SfoI	GGCGCC	1 cut(s)	6
SfuI	TTCGAA	1 cut(s)	823
SinI	GGWCC	5 cut(s)	287, 692, 716, 2201, 2250
SmiMI	CAYNNNNRTG	6 cut(s)	637, 1023, 1050, 1568, 1677, 2215
SmlI	CTYRAG	1 cut(s)	1404
SmoI	CTYRAG	1 cut(s)	1404
Sse9I	AATT	9 cut(s)	985, 1198, 1245, 1251, 1368, 1581, 1822, 1868, 2245
SsiI	CCGC	6 cut(s)	122, 140, 208, 690, 1767, 1980
SspDI	GGCGCC	1 cut(s)	4
SspI	AATATT	3 cut(s)	391, 828, 2131
SspMI	CTAG	6 cut(s)	720, 1241, 1290, 1685, 2148, 2407
SstI	GAGCTC	1 cut(s)	600
StyI	CCWWGG	3 cut(s)	765, 877, 1178
TaaI	ACNGT	5 cut(s)	319, 913, 1495, 2050, 2088
TaiI	ACGT	4 cut(s)	30, 180, 335, 1060
TasI	AATT	9 cut(s)	985, 1198, 1245, 1251, 1368, 1581, 1822, 1868, 2245
TauI	GCSGC	3 cut(s)	124, 142, 210
TfiI	GAWTC	6 cut(s)	97, 150, 416, 1162, 1817, 2063
Tru1I	TTAA	6 cut(s)	582, 972, 1079, 1367, 1430, 2000
Tru9I	TTAA	6 cut(s)	582, 972, 1079, 1367, 1430, 2000
TscAI	CASTG	2 cut(s)	918, 1640
TseFI	GTSAC	3 cut(s)	845, 1591, 1631
TseI	GCWGC	5 cut(s)	239, 779, 1042, 1226, 1574
Tsp45I	GTSAC	3 cut(s)	845, 1591, 1631
TspDTI	ATGAA	8 cut(s)	103, 613, 641, 1078, 1316, 1330, 1464, 2340
TspGWI	ACGGA	1 cut(s)	423
TspRI	CASTG	2 cut(s)	918, 1640
VneI	GTGCAC	2 cut(s)	89, 1728
VpaK11BI	GGWCC	5 cut(s)	287, 692, 716, 2201, 2250
XceI	RCATGY	2 cut(s)	109, 1850
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1380
XmiI	GTMKAC	1 cut(s)	937
XmnI	GAANNNNTTC	1 cut(s)	829
XspI	CTAG	6 cut(s)	720, 1241, 1290, 1685, 2148, 2407

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.