Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0005119

Physical Location & Seq

Genomic Coordinates Reverse (-)

8381191 .. 8390064

Gene Structure 8874 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1117900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2613 bp

ATGGCGTTGGGATTTAGCTCCGGCGACGCGCCCGGCTTCAAGTTGCTGCTCATTCTGGTAGCGACGTACGGCCTCATGGCGGCGCTGGTTCACTCCGTACTCTACATGCGCTTCATCAAGCCGCTCGACATCGACGCCCCTCTCGATCGCTTCTCCGAAGCCAGAGCCGTCGAGCACGTTCGAGTGTTGGCCCATGAGATCGACGGCCGCCAGGAAGGACGCCCTGGCTTGACCGAAGCTGCTCGGTACATTACAGGGCAGTTGGAAATGATAAAGGAGCGAGCTGGGTCCAATGTTAGAATTGAGATAGAAGAGTCCGTTGTTAATGGCACCTTTAATATGATGTTTTTGGGCCATGGCATTTCTCTTGGTTACAGAAACCACACAAATATTGTGATGAGGATATCGTCAATATATTCACAAGACAATGAACCATCAGTTTTGTTAAATGGACATTTTGACAGTCCACTTGCTTCCCCAGGTGCTGCTGATTGCGGTTCATGTGTAGCATCAATGCTGGAAATAGCAAGGCTAATGGTGGATTCTGGATGGGTTCCTCCTCGGCCTGTTATTTTTCTTTTTAATGGGGCTGAAGAGCTTTTCTTGTTGGGTTCTCATGGCTTCATGAAGACACATCAATGGCGTGAGAGTATTGGGGCTTTTATAAATGTGGAGGCATCAGGGACCGGCGGTCCTGATTTAGTTTGTCAATCAGGACCTGGTTCTTGGCCCTCTCAGGTCTATGCTCAATCAGCCGTATATCCCATGGCACATAGCGCAGCTCAGGATGTTTTTCCCGTCATTCCTGGAGATACAGATTTCCGGATATTTTCTCAAGATTATGGCAACATTCCAGGCTTGGATATTATCTTTCTCCTTGGTGGTTATTTTTATCATACATCCTATGATACAATGGAGAGATTATTACCTGGAAGTATGCAAGCTCGTGGAGAGAATTTGGTTAGTGTAATTAAGGCCTTCACCAATTCTTCTAAGCTACAAGTTAAACATGAAAGAGAGTCGAATGCATATCACTACGACGGAGAACATGCTGTTTTCTTTGATTATTTATCATTGTTTATGATTTACTATACGCGGAAGGTAGCCAGGCTACTTCACAGTATTCCTATTGGCATCTTCTTAGCTATGCCATTTTTTTCATACAAGCAGAATCGTGGGCTCCTTTCTTGGTTTTCAACTTTCTGCGATTTTATGAAAGGAATGATTTTCCATGCTACTGGGATTTTTCTGGCAATTGTATTTCCAATTATCTTTGCCATCCTGAGATTGCTGTTCACTAGTTGTGCTATGAATTGGTTTGCTCATCCATATTTGGCTTACTTGATGTTCATCCCGTGCTCACTTGTTGGTATGTTGATTCCTAGAATTATTTGGAGTAGCTTTCCCCTCTCTCAAGATGCATCAGCTCTCAAGTCTTTAAAGGAGGCTTTATCTGATGAAGCGAGGTTTTGGGGAGCATTTGGATTATACGCTGTATCAACTTTGGCTTACCTTTTAGCTGGGCTCAGCGGAGGCTTCTTGACTTTTTCAATGTCTACCTCTATGCTTCCTGGATGGATTTCATACTGCTTATCAGTCAAATTATTTGGTCACCAGTCACTCAGGTCAACATTATTTTACGTGTTACCTATACTTCCATGCCTTGCATACTCTGTATATTTTGGTGGATTTCTTATACAATTTGTGGTTGAAAAGCTGGGTATGATGGGTGCTTTGCCACCTCCATATGGTTATTTTGTTCCTGATGTTGTGATGGCAACAACAGTGGGAGCTGTGACTGGTTTGTGTGTAGGCCCTCTGATACCAATCTGTGGTCGCTGGTTAGCTAGGTCATCCATTTTGCAGGTTTTGCTGCACATTAGTGTGCTAGGCTTGGCTTTGTCGTCTCAGTTCTTTCCATACAGTGTGGACGCTCCTAAGCGGATTCTTTTCCAGCATACATTTCTTACTGCAGATGCAAATCAGGTTATGGACTCCAGCTATGATCTTTCTGTTGTAGATTCCAATTCTTTACTTTTTGTATTCAAACATGCACCGGAGGTGGCAAAGGAATTGCACATTAGTTCAGAGTTATCTTTTGAAACTGCAAAGTTATCTCAGCGGGAGAATTTTATGGGAATTTTTCCTGTGTCTTTTTTGTTCTCAAGAAGCTTGAAGTTCCCTGCAAAAAGTGACGGTATTTTGAAGCAATACAGGCAGTTCCCCCATCTGTCTACTTATGAGCCGCATACGGTTTTCATTGAGGGATCTCGGAGAGTATACTTGCAACTGTCTTTAGGTTCCTTAGAGGAGGTTTGGGTTACCGTCGTCAACATAACAGGCCCTTTATCCAGTTGGTCATTTGCAGACAATACACTTCCAGCTACTGTAACGAATGACGGAGGTCCACCGTCATACATATGCCGGCTTAGTGGAGCTAGCTCTGAGAATTGGAACTTCTGGTTAGAGGCTAGGGGATCTGACGGTTTGAGGGTTGACGTAGCTGTCATAGACCAAAAAATGGTTGACGAAACAAAGAAGCTGAGAGGCCTTTTTCCGGAGTGGGTGGATGTCGTTGCTTATTCAAGCTTCTTGTCAAGTTATATCTTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001541 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0007275 GO:0007548 GO:0008150 GO:0008406 GO:0008585 GO:0012505 GO:0022414 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0045137 GO:0046545 GO:0046660 GO:0048513 GO:0048608 GO:0048731 GO:0048856 GO:0061458

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_M28

Protein Analysis

871

Amino Acids

96.56

Weight (kDa)

6.09

Isoelectric Point (pI)

37.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M28	PF04389	130 - 322	7.1e-45	Peptidase family M28
ERMP1_C	PF22248	645 - 868	2.3e-30	Endoplasmic reticulum metallopeptidase 1, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005119)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G67420 AT1G67420 AT1G67420 AT1G67420 AT1G67420 AT1G67420
fragaria_vesca	FvH4_4g23910 FvH4_4g23910 FvH4_4g23910 FvH4_4g23910 FvH4_4g23910 FvH4_4g23910
malus_domestica	MD13G1117700.v1.1 MD16G1117900.v1.1
prunus_persica	Prupe.1G227300_v2.0.a1 Prupe.1G227300_v2.0.a1 Prupe.1G227300_v2.0.a1
pyrus_communis	pycom13g10180 pycom16g10100
rosa_chinensis	RchiOBHm_Chr4g0430551
rosa_laevigata	RLG00000006970
rosa_multiflora	Rmu_sc0011782.1_g000005
rosa_roxburghii	Rroxscaffold_5G00372320
rosa_rugosa	Rorug04G0244100 Rorug04G0244200
rosa_samantha	Rh4AG300200 Rh4BG307300 Rh4CG324000 Rh4DG304600
rosa_wichuraiana	Rw4G026050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	665
AasI	GACNNNNNNGTC	1 cut(s)	2504
Acc36I	ACCTGC	1 cut(s)	1855
AccB1I	GGYRCC	1 cut(s)	329
AccB7I	CCANNNNNTGG	2 cut(s)	1832, 2521
AccBSI	CCGCTC	1 cut(s)	124
AccI	GTMKAC	3 cut(s)	1556, 2234, 2280
AccII	CGCG	2 cut(s)	29, 1096
AccIII	TCCGGA	2 cut(s)	822, 2557
AclWI	GGATC	2 cut(s)	2275, 2485
AcoI	YGGCCR	1 cut(s)	205
AcsI	RAATTY	3 cut(s)	955, 2128, 2139
AcuI	CTGAAG	1 cut(s)	612
AcyI	GRCGYC	2 cut(s)	135, 220
AfaI	GTAC	3 cut(s)	68, 99, 248
AfiI	CCNNNNNNNGG	5 cut(s)	79, 1748, 1832, 2521, 2557
AflIII	ACRYGT	1 cut(s)	1641
AgsI	TTSAA	9 cut(s)	40, 1197, 1551, 1712, 2047, 2102, 2176, 2206, 2586
AhdI	GACNNNNNGTC	1 cut(s)	690
AhlI	ACTAGT	1 cut(s)	1298
AjnI	CCWGG	9 cut(s)	210, 223, 478, 718, 805, 853, 928, 1106, 1570
AleI	CACNNNNGTG	1 cut(s)	1881
Alw21I	GWGCWC	2 cut(s)	177, 1361
Alw26I	GTCTC	1 cut(s)	1911
AlwI	GGATC	2 cut(s)	2275, 2485
AlwNI	CAGNNNCTG	3 cut(s)	485, 719, 2387
Aor13HI	TCCGGA	2 cut(s)	822, 2557
ApeKI	GCWGC	5 cut(s)	46, 239, 485, 779, 1873
ApoI	RAATTY	3 cut(s)	955, 2128, 2139
AspLEI	GCGC	4 cut(s)	31, 85, 111, 779
AsuC2I	CCSGG	1 cut(s)	33
AsuHPI	GGTGA	2 cut(s)	973, 1604
AsuNHI	GCTAGC	1 cut(s)	2438
AvaII	GGWCC	5 cut(s)	288, 684, 692, 716, 2405
BanI	GGYRCC	1 cut(s)	329
BanII	GRGCYC	2 cut(s)	1182, 1527
BauI	CACGAG	1 cut(s)	945
BbsI	GAAGAC	1 cut(s)	635
Bbv12I	GWGCWC	2 cut(s)	177, 1361
BbvI	GCAGC	5 cut(s)	33, 226, 472, 791, 1860
BccI	CCATC	7 cut(s)	442, 543, 1286, 1569, 1720, 1768, 2235
BceAI	ACGGC	4 cut(s)	85, 152, 220, 740
BciT130I	CCWGG	9 cut(s)	212, 225, 480, 720, 807, 855, 930, 1108, 1572
BcnI	CCSGG	1 cut(s)	33
BcoDI	GTCTC	1 cut(s)	1911
BcuI	ACTAGT	1 cut(s)	1298
BfaI	CTAG	7 cut(s)	1299, 1383, 1848, 1889, 2439, 2472, 2611
BfmI	CTRYAG	1 cut(s)	1971
BfoI	RGCGCY	1 cut(s)	86
BfuAI	ACCTGC	1 cut(s)	1855
BisI	GCNGC	9 cut(s)	47, 81, 122, 208, 240, 486, 780, 1874, 2246
BlpI	GCTNAGC	1 cut(s)	1526
BlsI	GCNGC	9 cut(s)	48, 82, 123, 209, 241, 487, 781, 1875, 2247
Bme18I	GGWCC	5 cut(s)	288, 684, 692, 716, 2405
BmeRI	GACNNNNNGTC	1 cut(s)	690
BmiI	GGNNCC	6 cut(s)	289, 331, 555, 685, 1181, 2302
BmrI	ACTGGG	1 cut(s)	1248
BmsI	GCATC	6 cut(s)	518, 686, 1143, 1408, 1430, 1966
BmtI	GCTAGC	1 cut(s)	2442
BmuI	ACTGGG	1 cut(s)	1248
BoxI	GACNNNNGTC	1 cut(s)	2403
BpiI	GAAGAC	1 cut(s)	635
BpmI	CTGGAG	2 cut(s)	828, 1981
Bpu10I	CCTNAGC	2 cut(s)	783, 1938
Bpu1102I	GCTNAGC	1 cut(s)	1526
BpuEI	CTTGAG	4 cut(s)	819, 1398, 1415, 2149
BpuMI	CCSGG	1 cut(s)	33
BsaAI	YACGTR	1 cut(s)	1642
BsaBI	GATNNNNATC	3 cut(s)	1349, 1826, 1980
BsaHI	GRCGYC	2 cut(s)	135, 220
BsaJI	CCNNGG	6 cut(s)	223, 355, 478, 560, 765, 877
BsaWI	WCCGGW	3 cut(s)	822, 2056, 2557
Bsc4I	CCNNNNNNNGG	5 cut(s)	79, 1748, 1832, 2521, 2557
Bse118I	RCCGGY	2 cut(s)	686, 2424
Bse1I	ACTGG	4 cut(s)	1243, 1615, 1804, 2352
Bse8I	GATNNNNATC	3 cut(s)	1349, 1826, 1980
BseAI	TCCGGA	2 cut(s)	822, 2557
BseBI	CCWGG	9 cut(s)	212, 225, 480, 720, 807, 855, 930, 1108, 1572
BseDI	CCNNGG	6 cut(s)	223, 355, 478, 560, 765, 877
BseGI	GGATG	9 cut(s)	554, 793, 899, 1278, 1324, 1350, 1580, 1853, 2575
BseJI	GATNNNNATC	3 cut(s)	1349, 1826, 1980
BseLI	CCNNNNNNNGG	5 cut(s)	79, 1748, 1832, 2521, 2557
BseMII	CTCAG	9 cut(s)	749, 797, 1274, 1540, 1636, 1922, 2132, 2436, 2534
BseNI	ACTGG	4 cut(s)	1243, 1615, 1804, 2352
BseRI	GAGGAG	2 cut(s)	549, 2324
BseX3I	CGGCCG	1 cut(s)	205
BseXI	GCAGC	5 cut(s)	33, 226, 472, 791, 1860
BseYI	CCCAGC	3 cut(s)	284, 1520, 1717
BsgI	GTGCAG	1 cut(s)	1859
Bsh1236I	CGCG	2 cut(s)	29, 1096
Bsh1285I	CGRYCG	2 cut(s)	148, 208
BshNI	GGYRCC	1 cut(s)	329
BsiEI	CGRYCG	2 cut(s)	148, 208
BsiHKAI	GWGCWC	2 cut(s)	177, 1361
BsiSI	CCGG	7 cut(s)	21, 33, 687, 823, 2057, 2425, 2558
BsiWI	CGTACG	1 cut(s)	66
BslFI	GGGAC	1 cut(s)	697
BslI	CCNNNNNNNGG	5 cut(s)	79, 1748, 1832, 2521, 2557
BsmAI	GTCTC	1 cut(s)	1911
BsmBI	CGTCTC	1 cut(s)	1911
BsmFI	GGGAC	1 cut(s)	697
BsmI	GAATGC	1 cut(s)	1030
Bsp1286I	GDGCHC	4 cut(s)	177, 1182, 1361, 1527
Bsp13I	TCCGGA	2 cut(s)	822, 2557
Bsp143I	GATC	5 cut(s)	145, 198, 2005, 2267, 2477
Bsp1720I	GCTNAGC	1 cut(s)	1526
Bsp19I	CCATGG	2 cut(s)	355, 765
BspCNI	CTCAG	9 cut(s)	748, 796, 1275, 1539, 1635, 1921, 2131, 2437, 2535
BspEI	TCCGGA	2 cut(s)	822, 2557
BspFNI	CGCG	2 cut(s)	29, 1096
BspHI	TCATGA	1 cut(s)	624
BspLI	GGNNCC	6 cut(s)	289, 331, 555, 685, 1181, 2302
BspMAI	CTGCAG	1 cut(s)	1975
BspMI	ACCTGC	1 cut(s)	1855
BspOI	GCTAGC	1 cut(s)	2442
BspPI	GGATC	2 cut(s)	2275, 2485
BspQI	GCTCTTC	1 cut(s)	588
BspT107I	GGYRCC	1 cut(s)	329
BsrBI	CCGCTC	1 cut(s)	124
BsrFI	RCCGGY	2 cut(s)	686, 2424
BsrI	ACTGG	4 cut(s)	1243, 1615, 1804, 2352
BssAI	RCCGGY	2 cut(s)	686, 2424
BssECI	CCNNGG	6 cut(s)	223, 355, 478, 560, 765, 877
BssMI	GATC	5 cut(s)	145, 198, 2005, 2267, 2477
BssNAI	GTATAC	1 cut(s)	2281
BssNI	GRCGYC	2 cut(s)	135, 220
BssSI	CACGAG	1 cut(s)	945
BssT1I	CCWWGG	3 cut(s)	355, 765, 877
Bst1107I	GTATAC	1 cut(s)	2281
Bst2BI	CACGAG	1 cut(s)	945
Bst2UI	CCWGG	9 cut(s)	212, 225, 480, 720, 807, 855, 930, 1108, 1572
Bst6I	CTCTTC	2 cut(s)	306, 588
BstACI	GRCGYC	2 cut(s)	135, 220
BstAPI	GCANNNNNTGC	1 cut(s)	1870
BstBAI	YACGTR	1 cut(s)	1642
BstC8I	GCNNGC	4 cut(s)	282, 942, 2426, 2440
BstDSI	CCRYGG	2 cut(s)	355, 765
BstEII	GGTNACC	2 cut(s)	1610, 2320
BstF5I	GGATG	9 cut(s)	554, 793, 899, 1278, 1324, 1350, 1580, 1853, 2575
BstFNI	CGCG	2 cut(s)	29, 1096
BstH2I	RGCGCY	1 cut(s)	86
BstHHI	GCGC	4 cut(s)	31, 85, 111, 779
BstKTI	GATC	5 cut(s)	148, 201, 2008, 2270, 2480
BstMAI	GTCTC	1 cut(s)	1911
BstMBI	GATC	5 cut(s)	145, 198, 2005, 2267, 2477
BstMCI	CGRYCG	2 cut(s)	148, 208
BstMWI	GCNNNNNNNGC	4 cut(s)	752, 776, 1870, 2215
BstNI	CCWGG	9 cut(s)	212, 225, 480, 720, 807, 855, 930, 1108, 1572
BstNSI	RCATGY	3 cut(s)	109, 1052, 2054
BstPAI	GACNNNNGTC	1 cut(s)	2403
BstPI	GGTNACC	2 cut(s)	1610, 2320
BstSFI	CTRYAG	1 cut(s)	1971
BstUI	CGCG	2 cut(s)	29, 1096
BstV1I	GCAGC	5 cut(s)	33, 226, 472, 791, 1860
BstV2I	GAAGAC	1 cut(s)	635
BstX2I	RGATCY	2 cut(s)	2267, 2477
BstXI	CCANNNNNNTGG	1 cut(s)	1238
BstYI	RGATCY	2 cut(s)	2267, 2477
BstZ17I	GTATAC	1 cut(s)	2281
BstZI	CGGCCG	1 cut(s)	205
BtgI	CCRYGG	2 cut(s)	355, 765
BtsCI	GGATG	9 cut(s)	554, 793, 899, 1278, 1324, 1350, 1580, 1853, 2575
BtsIMutI	CAGTG	2 cut(s)	1791, 1930
BveI	ACCTGC	1 cut(s)	1855
Cac8I	GCNNGC	4 cut(s)	282, 942, 2426, 2440
CaiI	CAGNNNCTG	3 cut(s)	485, 719, 2387
CciI	TCATGA	1 cut(s)	624
CfoI	GCGC	4 cut(s)	31, 85, 111, 779
Cfr10I	RCCGGY	2 cut(s)	686, 2424
CseI	GACGC	4 cut(s)	35, 143, 228, 1940
CsiI	ACCWGGT	1 cut(s)	718
Csp6I	GTAC	3 cut(s)	67, 98, 247
CspCI	CAANNNNNGTGG	2 cut(s)	1767, 1802
CviQI	GTAC	3 cut(s)	67, 98, 247
DpnI	GATC	5 cut(s)	147, 200, 2007, 2269, 2479
DpnII	GATC	5 cut(s)	145, 198, 2005, 2267, 2477
DraI	TTTAAA	1 cut(s)	1440
DrdI	GACNNNNNNGTC	1 cut(s)	2504
DriI	GACNNNNNGTC	1 cut(s)	690
DseDI	GACNNNNNNGTC	1 cut(s)	2504
EaeI	YGGCCR	1 cut(s)	205
EagI	CGGCCG	1 cut(s)	205
Eam1104I	CTCTTC	2 cut(s)	306, 588
Eam1105I	GACNNNNNGTC	1 cut(s)	690
EarI	CTCTTC	2 cut(s)	306, 588
EclXI	CGGCCG	1 cut(s)	205
Eco130I	CCWWGG	3 cut(s)	355, 765, 877
Eco147I	AGGCCT	2 cut(s)	977, 2550
Eco24I	GRGCYC	2 cut(s)	1182, 1527
Eco32I	GATATC	1 cut(s)	405
Eco47I	GGWCC	5 cut(s)	288, 684, 692, 716, 2405
Eco52I	CGGCCG	1 cut(s)	205
Eco57I	CTGAAG	1 cut(s)	612
Eco91I	GGTNACC	2 cut(s)	1610, 2320
EcoO109I	RGGNCCY	3 cut(s)	716, 1814, 2342
EcoO65I	GGTNACC	2 cut(s)	1610, 2320
EcoRII	CCWGG	9 cut(s)	210, 223, 478, 718, 805, 853, 928, 1106, 1570
EcoRV	GATATC	1 cut(s)	405
EcoT14I	CCWWGG	3 cut(s)	355, 765, 877
EcoT22I	ATGCAT	2 cut(s)	1030, 1423
EcoT38I	GRGCYC	2 cut(s)	1182, 1527
ErhI	CCWWGG	3 cut(s)	355, 765, 877
Esp3I	CGTCTC	1 cut(s)	1911
FaqI	GGGAC	1 cut(s)	697
FauI	CCCGC	1 cut(s)	2115
FauNDI	CATATG	2 cut(s)	1747, 2420
FblI	GTMKAC	3 cut(s)	1556, 2234, 2280
Fnu4HI	GCNGC	9 cut(s)	47, 81, 122, 208, 240, 486, 780, 1874, 2246
FokI	GGATG	9 cut(s)	561, 800, 886, 1265, 1311, 1337, 1587, 1840, 2582
FriOI	GRGCYC	2 cut(s)	1182, 1527
Fsp4HI	GCNGC	9 cut(s)	47, 81, 122, 208, 240, 486, 780, 1874, 2246
FspBI	CTAG	7 cut(s)	1299, 1383, 1848, 1889, 2439, 2472, 2611
GlaI	GCGC	4 cut(s)	30, 84, 110, 778
GluI	GCNGC	9 cut(s)	47, 81, 122, 208, 240, 486, 780, 1874, 2246
GsaI	CCCAGC	3 cut(s)	288, 1524, 1721
GsuI	CTGGAG	2 cut(s)	828, 1981
HaeII	RGCGCY	1 cut(s)	86
HapII	CCGG	7 cut(s)	21, 33, 687, 823, 2057, 2425, 2558
HgaI	GACGC	4 cut(s)	35, 143, 228, 1940
HhaI	GCGC	4 cut(s)	31, 85, 111, 779
Hin1I	GRCGYC	2 cut(s)	135, 220
Hin6I	GCGC	4 cut(s)	29, 83, 109, 777
HinP1I	GCGC	4 cut(s)	29, 83, 109, 777
HincII	GTYRAC	4 cut(s)	1629, 2332, 2497, 2527
HindII	GTYRAC	4 cut(s)	1629, 2332, 2497, 2527
HindIII	AAGCTT	2 cut(s)	2170, 2587
HinfI	GANTC	8 cut(s)	314, 542, 1019, 1171, 1378, 1945, 1994, 2021
HpaII	CCGG	7 cut(s)	21, 33, 687, 823, 2057, 2425, 2558
HphI	GGTGA	2 cut(s)	973, 1604
Hpy188I	TCNGA	7 cut(s)	157, 1456, 1821, 2089, 2274, 2446, 2482
Hpy99I	CGWCG	7 cut(s)	29, 67, 137, 173, 206, 1043, 2330
HpyAV	CCTTC	3 cut(s)	209, 988, 1093
HpyCH4IV	ACGT	4 cut(s)	65, 177, 1641, 2499
HpyF10VI	GCNNNNNNNGC	4 cut(s)	752, 776, 1870, 2215
HpySE526I	ACGT	4 cut(s)	65, 177, 1641, 2499
Hsp92I	GRCGYC	2 cut(s)	135, 220
HspAI	GCGC	4 cut(s)	29, 83, 109, 777
Kpn2I	TCCGGA	2 cut(s)	822, 2557
KroI	GCCGGC	1 cut(s)	2424
KroNI	GCCGGC	1 cut(s)	2426
Kzo9I	GATC	5 cut(s)	145, 198, 2005, 2267, 2477
LguI	GCTCTTC	1 cut(s)	588
LmnI	GCTCC	7 cut(s)	23, 277, 1185, 1475, 1790, 1939, 2435
Lsp1109I	GCAGC	5 cut(s)	33, 226, 472, 791, 1860
LweI	GCATC	6 cut(s)	518, 686, 1143, 1408, 1430, 1966
MabI	ACCWGGT	1 cut(s)	718
MaeI	CTAG	7 cut(s)	1299, 1383, 1848, 1889, 2439, 2472, 2611
MaeII	ACGT	4 cut(s)	65, 177, 1641, 2499
MaeIII	GTNAC	8 cut(s)	371, 1610, 1617, 1644, 1795, 2192, 2320, 2389
MalI	GATC	5 cut(s)	147, 200, 2007, 2269, 2479
MbiI	CCGCTC	1 cut(s)	124
MboI	GATC	5 cut(s)	145, 198, 2005, 2267, 2477
MboII	GAAGA	6 cut(s)	323, 605, 640, 981, 1129, 2599
MfeI	CAATTG	1 cut(s)	1254
MflI	RGATCY	2 cut(s)	2267, 2477
MhlI	GDGCHC	4 cut(s)	177, 1182, 1361, 1527
MlyI	GAGTC	3 cut(s)	323, 1028, 1988
MmeI	TCCRAC	1 cut(s)	243
Mph1103I	ATGCAT	2 cut(s)	1030, 1423
MroI	TCCGGA	2 cut(s)	822, 2557
MroNI	GCCGGC	1 cut(s)	2424
MseI	TTAA	7 cut(s)	324, 336, 446, 582, 972, 1005, 1439
MslI	CAYNNNNRTG	4 cut(s)	637, 1881, 1883, 2419
MspA1I	CMGCKG	2 cut(s)	1530, 2122
MspI	CCGG	7 cut(s)	21, 33, 687, 823, 2057, 2425, 2558
MunI	CAATTG	1 cut(s)	1254
Mva1269I	GAATGC	1 cut(s)	1030
MvaI	CCWGG	9 cut(s)	212, 225, 480, 720, 807, 855, 930, 1108, 1572
MvnI	CGCG	2 cut(s)	29, 1096
MwoI	GCNNNNNNNGC	4 cut(s)	752, 776, 1870, 2215
NaeI	GCCGGC	1 cut(s)	2426
NciI	CCSGG	1 cut(s)	33
NcoI	CCATGG	2 cut(s)	355, 765
NdeI	CATATG	2 cut(s)	1747, 2420
NdeII	GATC	5 cut(s)	145, 198, 2005, 2267, 2477
NgoMIV	GCCGGC	1 cut(s)	2424
NheI	GCTAGC	1 cut(s)	2438
NlaIV	GGNNCC	6 cut(s)	289, 331, 555, 685, 1181, 2302
NmeAIII	GCCGAG	1 cut(s)	541
NmuCI	GTSAC	4 cut(s)	1610, 1617, 1795, 2192
NsiI	ATGCAT	2 cut(s)	1030, 1423
NspI	RCATGY	3 cut(s)	109, 1052, 2054
OliI	CACNNNNGTG	1 cut(s)	1881
PagI	TCATGA	1 cut(s)	624
PceI	AGGCCT	2 cut(s)	977, 2550
PciSI	GCTCTTC	1 cut(s)	588
PcsI	WCGNNNNNNNCGW	1 cut(s)	141
PctI	GAATGC	1 cut(s)	1030
PdiI	GCCGGC	1 cut(s)	2426
PfeI	GAWTC	5 cut(s)	542, 1171, 1378, 1945, 2021
Pfl23II	CGTACG	1 cut(s)	66
PflMI	CCANNNNNTGG	2 cut(s)	1832, 2521
PfoI	TCCNGGA	2 cut(s)	805, 1570
PkrI	GCNGC	9 cut(s)	48, 82, 123, 209, 241, 487, 781, 1875, 2247
Ple19I	CGATCG	1 cut(s)	148
PleI	GAGTC	3 cut(s)	322, 1027, 1988
PpsI	GAGTC	3 cut(s)	322, 1027, 1988
Ppu21I	YACGTR	1 cut(s)	1642
PpuMI	RGGWCCY	1 cut(s)	716
PshAI	GACNNNNGTC	1 cut(s)	2403
PsiI	TTATAA	1 cut(s)	665
Psp5II	RGGWCCY	1 cut(s)	716
Psp6I	CCWGG	9 cut(s)	210, 223, 478, 718, 805, 853, 928, 1106, 1570
PspEI	GGTNACC	2 cut(s)	1610, 2320
PspFI	CCCAGC	3 cut(s)	284, 1520, 1717
PspGI	CCWGG	9 cut(s)	210, 223, 478, 718, 805, 853, 928, 1106, 1570
PspLI	CGTACG	1 cut(s)	66
PspN4I	GGNNCC	6 cut(s)	289, 331, 555, 685, 1181, 2302
PspPPI	RGGWCCY	1 cut(s)	716
PstI	CTGCAG	1 cut(s)	1975
PstNI	CAGNNNCTG	3 cut(s)	485, 719, 2387
PsuI	RGATCY	2 cut(s)	2267, 2477
PvuI	CGATCG	1 cut(s)	148
RsaI	GTAC	3 cut(s)	68, 99, 248
RsaNI	GTAC	3 cut(s)	67, 98, 247
RseI	CAYNNNNRTG	4 cut(s)	637, 1881, 1883, 2419
SapI	GCTCTTC	1 cut(s)	588
SaqAI	TTAA	7 cut(s)	324, 336, 446, 582, 972, 1005, 1439
SatI	GCNGC	9 cut(s)	47, 81, 122, 208, 240, 486, 780, 1874, 2246
Sau3AI	GATC	5 cut(s)	145, 198, 2005, 2267, 2477
SchI	GAGTC	3 cut(s)	323, 1028, 1988
SduI	GDGCHC	4 cut(s)	177, 1182, 1361, 1527
SexAI	ACCWGGT	1 cut(s)	718
SfaNI	GCATC	6 cut(s)	518, 686, 1143, 1408, 1430, 1966
SfcI	CTRYAG	1 cut(s)	1971
SinI	GGWCC	5 cut(s)	288, 684, 692, 716, 2405
SmiMI	CAYNNNNRTG	4 cut(s)	637, 1881, 1883, 2419
SmlI	CTYRAG	4 cut(s)	834, 1413, 1430, 2164
SmoI	CTYRAG	4 cut(s)	834, 1413, 1430, 2164
SpeI	ACTAGT	1 cut(s)	1298
SseBI	AGGCCT	2 cut(s)	977, 2550
SspI	AATATT	1 cut(s)	391
SspMI	CTAG	7 cut(s)	1299, 1383, 1848, 1889, 2439, 2472, 2611
StuI	AGGCCT	2 cut(s)	977, 2550
StyI	CCWWGG	3 cut(s)	355, 765, 877
TaiI	ACGT	4 cut(s)	68, 180, 1644, 2502
TaqI	TCGA	7 cut(s)	126, 132, 144, 171, 181, 201, 1022
TaqII	GACCGA	1 cut(s)	248
TauI	GCSGC	4 cut(s)	83, 124, 210, 2248
TfiI	GAWTC	5 cut(s)	542, 1171, 1378, 1945, 2021
Tru1I	TTAA	7 cut(s)	324, 336, 446, 582, 972, 1005, 1439
Tru9I	TTAA	7 cut(s)	324, 336, 446, 582, 972, 1005, 1439
TscAI	CASTG	2 cut(s)	1791, 1930
TseFI	GTSAC	4 cut(s)	1610, 1617, 1795, 2192
TseI	GCWGC	5 cut(s)	46, 239, 485, 779, 1873
Tsp45I	GTSAC	4 cut(s)	1610, 1617, 1795, 2192
TspGWI	ACGGA	4 cut(s)	85, 307, 1056, 2415
TspRI	CASTG	2 cut(s)	1791, 1930
Van91I	CCANNNNNTGG	2 cut(s)	1832, 2521
VpaK11BI	GGWCC	5 cut(s)	288, 684, 692, 716, 2405
XapI	RAATTY	3 cut(s)	955, 2128, 2139
XceI	RCATGY	3 cut(s)	109, 1052, 2054
XmiI	GTMKAC	3 cut(s)	1556, 2234, 2280
XspI	CTAG	7 cut(s)	1299, 1383, 1848, 1889, 2439, 2472, 2611
Zsp2I	ATGCAT	2 cut(s)	1030, 1423

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.