Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0005119

Physical Location & Seq

Genomic Coordinates Forward (+)

52270392 .. 52277403

Gene Structure 7012 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G026050.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2601 bp

ATGGCGCCGAGGCTCGGTTCCGGCGATGTGTCCGGCTTCAAGTTCCTTCTATTTCTCGTTGCGATTTACGGCCTCATCGCCGTCCTGGTGCACTCGATTCTCCACATGCGCTTCATTACGCCGCTTGGGATCGACGCGCCGCTCGATCGATTCTCCGAGGCTCGAGCCGTCGAGCACGTTCGAGTCCTGGCCAAAGATATCGATAGCCGCCAGGAAGGGAGTCCTGGATTGAGAGAAGCTGCTCGATATATTACAGCGCAGTTGGAGATGGTAAAGGAGCGAGCTGGACCTAACTTTAGAGTTGAGATAGAAGAGACTGTTGTGAATGGCACATTTAATATGCTGTTTTTAGGCTATAGCATATCTCTGGGGTACAGAAACCACACAAATATTGTGATGAGAATATCATCAGTAGATTCACAAGACTCTGATCGATCAGTATTGTTGAATGGCCATTTTGATAGTCCACTTGGTTCTCCGGGTGCTGGTGATTGTGGGTCATGTGTAGCATCAATGCTGGAAATAGCAAGACTTATTGTGGACTCTGGATGGGTTCCCCCTCAGCCTGTTATTTTTCTTTTTAATGGTGCAGAAGAGCTCTTTTTGCTGGGGTCTCATGGCTTCATGAAGACACATAAATGGCGTGAAACTATTGGGGCTTTTATAAATGTGGAAGCGTCGGGGACAGGCGGTCCTGATTTAGTTTGCCAATCAGGACCTAGTTCCTGGCCTTCTCAGGTCTATGCTCAATCAGCAGTATATCCCATGGCACATAGCGCAGCCCAGGATGTTTTTCCTGTTGTTCCAGGAGATACAGATTTTCGAATATTTTCCGAGGACTATGGTGACATTCCTGGCTTGGATATTATCTTTCTCCTTGGTGGTTATTTTTATCATACATCCTTCGATACAGTGGAGAGACTATTACCTGGAAGTATACAAGCTCGTGGAGAAAATCTGTTTAGCGTACTTAAAGCGTTCACCAATTCTTCTACGCTACAAAATACTCTCGAAAGACATTCTAATGTATCAACTGCCAAGCAGCATGAGGATGGACGTGCTGTTTTCTTTGATTATTTAACATGGTTCATGCTATGGTACTTCATCCCTATTCCTATTGGCATCTTCCTGGCCATGCCATTTTTCTCACAAAAGCAGAATCCTGGGCTCCTACCTTGGTTTGCTACTTTCAGCAATTTTGTAAAAGGAATGCTTTTCCATGCTGCTGGGGTTTTTCTAGCAATTGTAATTCCGGTTATCTTTTCCATTGTGAGATTGTTGTTTGCTAGTCGTGCAATGAACTGGTTTGCTCATCCATATTTGGCTTATTTGATGTTCATACCTTGCTCTCTTGTTGGTCTGTTAATTCCAAGATTTGTTTGGAGTAGCTTTCCCCTCACTCAAGAGGCATCAGTTGTCAAGTCTTTAAAAGAGGCATTATCGGATGAAGCAAGGTTCTGGGGAGCTTTTGGATTTTACGCTACAATAACTGTGGCTTACCTGTATGCTGGGTTGAGCGGAGGCTTCTTGACTTTTTCACTGTCCGTTTCTATGCTTCTTGGATGGATTTCATATTGCTTAGCAGTCAAATTATTTGGTCGCCAATCACTCAGGTCAACGTTAGTTTATATGTTACCTATACTTCCATGCCTTGCGTACTCTGTGTATTTTGGTGCATTTCTTGTCCAGTTTTTGGTTGAAAAGATGGGTATGATGGGTGCTTTGCCACCTCCATATGGTTATTTCGTTCCTGACATTGTGGTGGCTGCAGTAATTGGAGTTGTGACCAGTTGGTCTGTAGGCCCTCTGATACCTATTTGCGGTCACTGGTTAGCGAGGTCATCCATTTTGCAAGTCTTGTTGCATCTTAGTGTGCTAGGCTTGGCTCTGTCATCTCAGTTCTTTCCATATAGCACGGGTGCACCTAAGAGAGTTGTTTTCCAGCATACTTTTCTTACTGCAGATGCAAATCATGTTGTGGAGTCCAGCTATGATTTTTCTGTTGTGGATTCCAATTCATTACTTTTTCTTTTCAAACATGCACCCGAGGTGGCAAAGGAATTGCACATTAGTTCAGAGTTGTCTTTTGAAACTGCAAAGTCATCTCACCGAGAGACTTGGATGGGACTTTTTCCTGTTTCTTTTTTGTTTTCAAGAAGCTTGAAGTTCCCCGCAAGAAGTGATGGTATTTTAAAGCAATACGAGCAGTTCCCTCATCTGTCCACTTATAAGCCACATACAGTATTTGGCGAGGAATCTCGTAGAATATACTTGGAACTGTCTTTAGGTTCCTTAAAGGAGGTTTGGGTTGCAGTCCTCAATATTACAGGCCCTTTATCTAGTTGGTCATTTGCAGACAATACACTTTCAGCTACTGAAACATCTGATGGAGGTCCACCATCATACATATGCCGACTTAGTGGAGCAAGTCCTGAGAATTGGACCTTCTGGTTAGAGGCTAACAGTTCTGAAGATCTAAGGGTAGAAGTTGCTGTTGTAGATCAATATATGGTTGATGAAGCAAAGAAGCTGAAAGGGCTTTTTCCGGAGTGGGTGGATGTAGTTGCTTATTCGGGCTTTTTGTCGAGTTATATTTTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001541 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0007275 GO:0007548 GO:0008150 GO:0008406 GO:0008585 GO:0012505 GO:0022414 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0045137 GO:0046545 GO:0046660 GO:0048513 GO:0048608 GO:0048731 GO:0048856 GO:0061458

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_M28

Protein Analysis

866

Amino Acids

96.04

Weight (kDa)

5.83

Isoelectric Point (pI)

36.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M28	PF04389	130 - 322	2.2e-44	Peptidase family M28
ERMP1_C	PF22248	641 - 864	1.6e-31	Endoplasmic reticulum metallopeptidase 1, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005119)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G67420 AT1G67420 AT1G67420 AT1G67420 AT1G67420 AT1G67420
fragaria_vesca	FvH4_4g23910 FvH4_4g23910 FvH4_4g23910 FvH4_4g23910 FvH4_4g23910 FvH4_4g23910
malus_domestica	MD13G1117700.v1.1 MD16G1117900.v1.1
prunus_persica	Prupe.1G227300_v2.0.a1 Prupe.1G227300_v2.0.a1 Prupe.1G227300_v2.0.a1
pyrus_communis	pycom13g10180 pycom16g10100
rosa_chinensis	RchiOBHm_Chr4g0430551
rosa_laevigata	RLG00000006970
rosa_multiflora	Rmu_sc0011782.1_g000005
rosa_roxburghii	Rroxscaffold_5G00372320
rosa_rugosa	Rorug04G0244100 Rorug04G0244200
rosa_samantha	Rh4AG300200 Rh4BG307300 Rh4CG324000 Rh4DG304600
rosa_wichuraiana	Rw4G026050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	665, 2229
AasI	GACNNNNNNGTC	1 cut(s)	1792
AccB1I	GGYRCC	1 cut(s)	4
AccB7I	CCANNNNNTGG	1 cut(s)	1693
AccBSI	CCGCTC	2 cut(s)	142, 1518
AccI	GTMKAC	1 cut(s)	937
AccII	CGCG	1 cut(s)	137
AccIII	TCCGGA	1 cut(s)	2545
AciI	CCGC	7 cut(s)	122, 140, 208, 690, 1518, 1821, 2172
AclI	AACGTT	1 cut(s)	1619
AclWI	GGATC	1 cut(s)	137
AcoI	YGGCCR	3 cut(s)	189, 451, 1131
AcuI	CTGAAG	1 cut(s)	2490
AcyI	GRCGYC	1 cut(s)	5
AfaI	GTAC	4 cut(s)	374, 969, 1100, 1658
AfiI	CCNNNNNNNGG	5 cut(s)	14, 485, 1693, 1736, 1820
AgsI	TTSAA	7 cut(s)	40, 448, 1700, 2035, 2090, 2154, 2164
AhdI	GACNNNNNGTC	1 cut(s)	690
AjiI	CACGTC	1 cut(s)	1058
Alw21I	GWGCWC	4 cut(s)	93, 177, 600, 1924
Alw26I	GTCTC	4 cut(s)	308, 618, 913, 2108
Alw44I	GTGCAC	2 cut(s)	89, 1920
AlwI	GGATC	1 cut(s)	137
AlwNI	CAGNNNCTG	1 cut(s)	2375
Ama87I	CYCGRG	2 cut(s)	162, 2045
Aor13HI	TCCGGA	1 cut(s)	2545
AoxI	GGCC	7 cut(s)	70, 189, 451, 728, 1131, 1801, 2329
ApaLI	GTGCAC	2 cut(s)	89, 1920
ApeKI	GCWGC	5 cut(s)	239, 779, 1042, 1223, 1766
Asp700I	GAANNNNTTC	1 cut(s)	829
AspLEI	GCGC	5 cut(s)	7, 111, 139, 259, 779
AspS9I	GGNCC	7 cut(s)	287, 692, 716, 1802, 2330, 2393, 2442
AsuC2I	CCSGG	1 cut(s)	480
AsuHPI	GGTGA	4 cut(s)	500, 857, 973, 2099
AsuII	TTCGAA	1 cut(s)	823
AvaI	CYCGRG	2 cut(s)	162, 2045
AvaII	GGWCC	5 cut(s)	287, 692, 716, 2393, 2442
BaeGI	GKGCMC	2 cut(s)	93, 1924
BalI	TGGCCA	3 cut(s)	191, 453, 1133
BanI	GGYRCC	1 cut(s)	4
BanII	GRGCYC	2 cut(s)	600, 1170
BauI	CACGAG	1 cut(s)	945
BbsI	GAAGAC	1 cut(s)	635
Bbv12I	GWGCWC	4 cut(s)	93, 177, 600, 1924
BbvCI	CCTCAGC	1 cut(s)	561
BbvI	GCAGC	5 cut(s)	226, 791, 1054, 1210, 1753
BceAI	ACGGC	3 cut(s)	65, 85, 152
BcnI	CCSGG	1 cut(s)	480
BcoDI	GTCTC	4 cut(s)	308, 618, 913, 2108
BfaI	CTAG	6 cut(s)	720, 1238, 1287, 1877, 2340, 2599
BfmI	CTRYAG	4 cut(s)	355, 1767, 1797, 1959
BfoI	RGCGCY	1 cut(s)	8
BglII	AGATCT	1 cut(s)	2473
BisI	GCNGC	8 cut(s)	122, 140, 208, 240, 780, 1043, 1224, 1767
BlpI	GCTNAGC	1 cut(s)	1579
BlsI	GCNGC	8 cut(s)	123, 141, 209, 241, 781, 1044, 1225, 1768
Bme18I	GGWCC	5 cut(s)	287, 692, 716, 2393, 2442
BmeRI	GACNNNNNGTC	1 cut(s)	690
BmeT110I	CYCGRG	2 cut(s)	162, 2045
BmgBI	CACGTC	1 cut(s)	1058
BmgT120I	GGNCC	7 cut(s)	287, 692, 716, 1802, 2330, 2393, 2442
BmiI	GGNNCC	5 cut(s)	6, 19, 555, 1169, 2290
BmsI	GCATC	5 cut(s)	518, 1131, 1418, 1873, 1954
BpiI	GAAGAC	1 cut(s)	635
Bpu10I	CCTNAGC	1 cut(s)	561
Bpu1102I	GCTNAGC	1 cut(s)	1579
Bpu14I	TTCGAA	1 cut(s)	823
BpuEI	CTTGAG	1 cut(s)	1386
BpuMI	CCSGG	1 cut(s)	480
Bsa29I	ATCGAT	3 cut(s)	148, 201, 433
BsaBI	GATNNNNATC	1 cut(s)	1968
BsaHI	GRCGYC	1 cut(s)	5
BsaI	GGTCTC	1 cut(s)	618
BsaJI	CCNNGG	9 cut(s)	8, 156, 765, 783, 834, 877, 1163, 1175, 2046
BsaWI	WCCGGW	2 cut(s)	1252, 2545
Bsc4I	CCNNNNNNNGG	5 cut(s)	14, 485, 1693, 1736, 1820
Bse1I	ACTGG	4 cut(s)	1307, 1687, 1788, 1832
Bse3DI	GCAATG	1 cut(s)	1302
Bse8I	GATNNNNATC	1 cut(s)	1968
BseAI	TCCGGA	1 cut(s)	2545
BseCI	ATCGAT	3 cut(s)	148, 201, 433
BseDI	CCNNGG	9 cut(s)	8, 156, 765, 783, 834, 877, 1163, 1175, 2046
BseJI	GATNNNNATC	1 cut(s)	1968
BseLI	CCNNNNNNNGG	5 cut(s)	14, 485, 1693, 1736, 1820
BseMI	GCAATG	1 cut(s)	1302
BseMII	CTCAG	5 cut(s)	575, 749, 1624, 1910, 2424
BseNI	ACTGG	4 cut(s)	1307, 1687, 1788, 1832
BseSI	GKGCMC	2 cut(s)	93, 1924
BseXI	GCAGC	5 cut(s)	226, 791, 1054, 1210, 1753
BseYI	CCCAGC	3 cut(s)	607, 1226, 1508
BsgI	GTGCAG	1 cut(s)	609
Bsh1236I	CGCG	1 cut(s)	137
Bsh1285I	CGRYCG	1 cut(s)	148
BshFI	GGCC	7 cut(s)	72, 191, 453, 730, 1133, 1803, 2331
BshNI	GGYRCC	1 cut(s)	4
BshVI	ATCGAT	3 cut(s)	148, 201, 433
BsiEI	CGRYCG	1 cut(s)	148
BsiHKAI	GWGCWC	4 cut(s)	93, 177, 600, 1924
BsiHKCI	CYCGRG	2 cut(s)	162, 2045
BsiSI	CCGG	5 cut(s)	21, 33, 479, 1253, 2546
BslFI	GGGAC	2 cut(s)	697, 2139
BslI	CCNNNNNNNGG	5 cut(s)	14, 485, 1693, 1736, 1820
BsmAI	GTCTC	4 cut(s)	308, 618, 913, 2108
BsmFI	GGGAC	2 cut(s)	697, 2139
BsmI	GAATGC	1 cut(s)	1215
BsnI	GGCC	7 cut(s)	72, 191, 453, 730, 1133, 1803, 2331
Bso31I	GGTCTC	1 cut(s)	618
BsoBI	CYCGRG	2 cut(s)	162, 2045
Bsp119I	TTCGAA	1 cut(s)	823
Bsp1286I	GDGCHC	5 cut(s)	93, 177, 600, 1170, 1924
Bsp13I	TCCGGA	1 cut(s)	2545
Bsp143I	GATC	6 cut(s)	129, 145, 430, 434, 2473, 2500
Bsp1720I	GCTNAGC	1 cut(s)	1579
Bsp19I	CCATGG	1 cut(s)	765
BspACI	CCGC	7 cut(s)	122, 140, 208, 690, 1518, 1821, 2172
BspANI	GGCC	7 cut(s)	72, 191, 453, 730, 1133, 1803, 2331
BspCNI	CTCAG	5 cut(s)	574, 748, 1623, 1909, 2425
BspDI	ATCGAT	3 cut(s)	148, 201, 433
BspEI	TCCGGA	1 cut(s)	2545
BspFNI	CGCG	1 cut(s)	137
BspHI	TCATGA	1 cut(s)	624
BspLI	GGNNCC	5 cut(s)	6, 19, 555, 1169, 2290
BspMAI	CTGCAG	2 cut(s)	1771, 1963
BspPI	GGATC	1 cut(s)	137
BspQI	GCTCTTC	1 cut(s)	588
BspT104I	TTCGAA	1 cut(s)	823
BspT107I	GGYRCC	1 cut(s)	4
BspTNI	GGTCTC	1 cut(s)	618
BsrBI	CCGCTC	2 cut(s)	142, 1518
BsrDI	GCAATG	1 cut(s)	1302
BsrI	ACTGG	4 cut(s)	1307, 1687, 1788, 1832
BssECI	CCNNGG	9 cut(s)	8, 156, 765, 783, 834, 877, 1163, 1175, 2046
BssMI	GATC	6 cut(s)	129, 145, 430, 434, 2473, 2500
BssNAI	GTATAC	1 cut(s)	938
BssNI	GRCGYC	1 cut(s)	5
BssSI	CACGAG	1 cut(s)	945
BssT1I	CCWWGG	3 cut(s)	765, 877, 1175
Bst1107I	GTATAC	1 cut(s)	938
Bst2BI	CACGAG	1 cut(s)	945
Bst4CI	ACNGT	7 cut(s)	319, 913, 1492, 1542, 2242, 2280, 2465
Bst6I	CTCTTC	2 cut(s)	306, 588
BstACI	GRCGYC	1 cut(s)	5
BstBI	TTCGAA	1 cut(s)	823
BstC8I	GCNNGC	1 cut(s)	282
BstDSI	CCRYGG	1 cut(s)	765
BstFNI	CGCG	1 cut(s)	137
BstH2I	RGCGCY	1 cut(s)	8
BstHHI	GCGC	5 cut(s)	7, 111, 139, 259, 779
BstKTI	GATC	6 cut(s)	132, 148, 433, 437, 2476, 2503
BstMAI	GTCTC	4 cut(s)	308, 618, 913, 2108
BstMBI	GATC	6 cut(s)	129, 145, 430, 434, 2473, 2500
BstMCI	CGRYCG	1 cut(s)	148
BstMWI	GCNNNNNNNGC	6 cut(s)	604, 752, 776, 1292, 2203, 2536
BstNSI	RCATGY	2 cut(s)	109, 2042
BstSFI	CTRYAG	4 cut(s)	355, 1767, 1797, 1959
BstSLI	GKGCMC	2 cut(s)	93, 1924
BstUI	CGCG	1 cut(s)	137
BstV1I	GCAGC	5 cut(s)	226, 791, 1054, 1210, 1753
BstV2I	GAAGAC	1 cut(s)	635
BstX2I	RGATCY	1 cut(s)	2473
BstXI	CCANNNNNNTGG	1 cut(s)	1226
BstYI	RGATCY	1 cut(s)	2473
BstZ17I	GTATAC	1 cut(s)	938
Bsu15I	ATCGAT	3 cut(s)	148, 201, 433
BsuRI	GGCC	7 cut(s)	72, 191, 453, 730, 1133, 1803, 2331
BsuTUI	ATCGAT	3 cut(s)	148, 201, 433
BtgI	CCRYGG	1 cut(s)	765
BtgZI	GCGATG	2 cut(s)	39, 61
BtrI	CACGTC	1 cut(s)	1058
BtsIMutI	CAGTG	3 cut(s)	918, 1538, 1825
Cac8I	GCNNGC	1 cut(s)	282
CaiI	CAGNNNCTG	1 cut(s)	2375
CciI	TCATGA	1 cut(s)	624
CfoI	GCGC	5 cut(s)	7, 111, 139, 259, 779
Cfr13I	GGNCC	7 cut(s)	287, 692, 716, 1802, 2330, 2393, 2442
ClaI	ATCGAT	3 cut(s)	148, 201, 433
CseI	GACGC	2 cut(s)	143, 666
Csp6I	GTAC	4 cut(s)	373, 968, 1099, 1657
CspCI	CAANNNNNGTGG	2 cut(s)	1473, 1508
CviQI	GTAC	4 cut(s)	373, 968, 1099, 1657
DinI	GGCGCC	1 cut(s)	6
DpnI	GATC	6 cut(s)	131, 147, 432, 436, 2475, 2502
DpnII	GATC	6 cut(s)	129, 145, 430, 434, 2473, 2500
DraI	TTTAAA	2 cut(s)	1428, 2193
DrdI	GACNNNNNNGTC	1 cut(s)	1792
DriI	GACNNNNNGTC	1 cut(s)	690
DseDI	GACNNNNNNGTC	1 cut(s)	1792
EaeI	YGGCCR	3 cut(s)	189, 451, 1131
Eam1104I	CTCTTC	2 cut(s)	306, 588
Eam1105I	GACNNNNNGTC	1 cut(s)	690
EarI	CTCTTC	2 cut(s)	306, 588
Ecl136II	GAGCTC	1 cut(s)	598
Eco130I	CCWWGG	3 cut(s)	765, 877, 1175
Eco24I	GRGCYC	2 cut(s)	600, 1170
Eco31I	GGTCTC	1 cut(s)	618
Eco32I	GATATC	1 cut(s)	199
Eco47I	GGWCC	5 cut(s)	287, 692, 716, 2393, 2442
Eco53kI	GAGCTC	1 cut(s)	598
Eco57I	CTGAAG	1 cut(s)	2490
Eco88I	CYCGRG	2 cut(s)	162, 2045
EcoICRI	GAGCTC	1 cut(s)	598
EcoO109I	RGGNCCY	3 cut(s)	716, 1802, 2330
EcoRV	GATATC	1 cut(s)	199
EcoT14I	CCWWGG	3 cut(s)	765, 877, 1175
EcoT38I	GRGCYC	2 cut(s)	600, 1170
EgeI	GGCGCC	1 cut(s)	6
EheI	GGCGCC	1 cut(s)	6
ErhI	CCWWGG	3 cut(s)	765, 877, 1175
FalI	AAGNNNNNCTT	2 cut(s)	1197, 1229
FaqI	GGGAC	2 cut(s)	697, 2139
FauI	CCCGC	1 cut(s)	2179
FauNDI	CATATG	2 cut(s)	1735, 2408
FblI	GTMKAC	1 cut(s)	937
Fnu4HI	GCNGC	8 cut(s)	122, 140, 208, 240, 780, 1043, 1224, 1767
FriOI	GRGCYC	2 cut(s)	600, 1170
Fsp4HI	GCNGC	8 cut(s)	122, 140, 208, 240, 780, 1043, 1224, 1767
FspBI	CTAG	6 cut(s)	720, 1238, 1287, 1877, 2340, 2599
GlaI	GCGC	5 cut(s)	6, 110, 138, 258, 778
GluI	GCNGC	8 cut(s)	122, 140, 208, 240, 780, 1043, 1224, 1767
GsaI	CCCAGC	3 cut(s)	611, 1230, 1512
HaeII	RGCGCY	1 cut(s)	8
HaeIII	GGCC	7 cut(s)	72, 191, 453, 730, 1133, 1803, 2331
HapII	CCGG	5 cut(s)	21, 33, 479, 1253, 2546
HgaI	GACGC	2 cut(s)	143, 666
HhaI	GCGC	5 cut(s)	7, 111, 139, 259, 779
Hin1I	GRCGYC	1 cut(s)	5
Hin6I	GCGC	5 cut(s)	5, 109, 137, 257, 777
HinP1I	GCGC	5 cut(s)	5, 109, 137, 257, 777
HincII	GTYRAC	1 cut(s)	1617
HindII	GTYRAC	1 cut(s)	1617
HindIII	AAGCTT	1 cut(s)	2158
HpaII	CCGG	5 cut(s)	21, 33, 479, 1253, 2546
HphI	GGTGA	4 cut(s)	500, 857, 973, 2099
Hpy166II	GTNNAC	9 cut(s)	91, 467, 541, 938, 981, 1617, 1922, 2223, 2396
Hpy188I	TCNGA	8 cut(s)	157, 430, 835, 1444, 1809, 2077, 2386, 2470
Hpy8I	GTNNAC	9 cut(s)	91, 467, 541, 938, 981, 1617, 1922, 2223, 2396
Hpy99I	CGWCG	3 cut(s)	137, 173, 682
HpyAV	CCTTC	5 cut(s)	56, 209, 741, 913, 2455
HpyCH4III	ACNGT	7 cut(s)	319, 913, 1492, 1542, 2242, 2280, 2465
HpyCH4IV	ACGT	3 cut(s)	177, 1057, 1619
HpyF10VI	GCNNNNNNNGC	6 cut(s)	604, 752, 776, 1292, 2203, 2536
HpySE526I	ACGT	3 cut(s)	177, 1057, 1619
Hsp92I	GRCGYC	1 cut(s)	5
HspAI	GCGC	5 cut(s)	5, 109, 137, 257, 777
KasI	GGCGCC	1 cut(s)	4
Kpn2I	TCCGGA	1 cut(s)	2545
Kzo9I	GATC	6 cut(s)	129, 145, 430, 434, 2473, 2500
LguI	GCTCTTC	1 cut(s)	588
LmnI	GCTCC	4 cut(s)	277, 1173, 1463, 2423
Lsp1109I	GCAGC	5 cut(s)	226, 791, 1054, 1210, 1753
LweI	GCATC	5 cut(s)	518, 1131, 1418, 1873, 1954
MaeI	CTAG	6 cut(s)	720, 1238, 1287, 1877, 2340, 2599
MaeII	ACGT	3 cut(s)	177, 1057, 1619
MaeIII	GTNAC	4 cut(s)	845, 1632, 1783, 1823
MalI	GATC	6 cut(s)	131, 147, 432, 436, 2475, 2502
MbiI	CCGCTC	2 cut(s)	142, 1518
MboI	GATC	6 cut(s)	129, 145, 430, 434, 2473, 2500
MboII	GAAGA	6 cut(s)	323, 605, 640, 981, 1117, 2483
MfeI	CAATTG	1 cut(s)	1242
MflI	RGATCY	1 cut(s)	2473
MhlI	GDGCHC	5 cut(s)	93, 177, 600, 1170, 1924
MlsI	TGGCCA	3 cut(s)	191, 453, 1133
MluNI	TGGCCA	3 cut(s)	191, 453, 1133
Mly113I	GGCGCC	1 cut(s)	5
MlyI	GAGTC	5 cut(s)	192, 229, 419, 536, 1991
MmeI	TCCRAC	1 cut(s)	243
Mox20I	TGGCCA	3 cut(s)	191, 453, 1133
MroI	TCCGGA	1 cut(s)	2545
MroXI	GAANNNNTTC	1 cut(s)	829
MscI	TGGCCA	3 cut(s)	191, 453, 1133
MseI	TTAA	8 cut(s)	336, 582, 972, 1079, 1364, 1427, 2192, 2294
MslI	CAYNNNNRTG	6 cut(s)	637, 1023, 1050, 1760, 1869, 2407
Msp20I	TGGCCA	3 cut(s)	191, 453, 1133
MspI	CCGG	5 cut(s)	21, 33, 479, 1253, 2546
MunI	CAATTG	1 cut(s)	1242
Mva1269I	GAATGC	1 cut(s)	1215
MvnI	CGCG	1 cut(s)	137
MwoI	GCNNNNNNNGC	6 cut(s)	604, 752, 776, 1292, 2203, 2536
NarI	GGCGCC	1 cut(s)	5
NciI	CCSGG	1 cut(s)	480
NcoI	CCATGG	1 cut(s)	765
NdeI	CATATG	2 cut(s)	1735, 2408
NdeII	GATC	6 cut(s)	129, 145, 430, 434, 2473, 2500
NlaIV	GGNNCC	5 cut(s)	6, 19, 555, 1169, 2290
NmeAIII	GCCGAG	1 cut(s)	33
NmuCI	GTSAC	3 cut(s)	845, 1783, 1823
NspI	RCATGY	2 cut(s)	109, 2042
NspV	TTCGAA	1 cut(s)	823
PaeR7I	CTCGAG	1 cut(s)	162
PagI	TCATGA	1 cut(s)	624
PciSI	GCTCTTC	1 cut(s)	588
PcsI	WCGNNNNNNNCGW	2 cut(s)	21, 141
PctI	GAATGC	1 cut(s)	1215
PdmI	GAANNNNTTC	1 cut(s)	829
PfeI	GAWTC	6 cut(s)	97, 150, 416, 1159, 2009, 2255
PflMI	CCANNNNNTGG	1 cut(s)	1693
PfoI	TCCNGGA	2 cut(s)	223, 805
PkrI	GCNGC	8 cut(s)	123, 141, 209, 241, 781, 1044, 1225, 1768
Ple19I	CGATCG	1 cut(s)	148
PleI	GAGTC	5 cut(s)	191, 228, 419, 536, 1990
PluTI	GGCGCC	1 cut(s)	8
PpsI	GAGTC	5 cut(s)	191, 228, 419, 536, 1990
PpuMI	RGGWCCY	1 cut(s)	716
PsiI	TTATAA	2 cut(s)	665, 2229
Psp124BI	GAGCTC	1 cut(s)	600
Psp1406I	AACGTT	1 cut(s)	1619
Psp5II	RGGWCCY	1 cut(s)	716
PspFI	CCCAGC	3 cut(s)	607, 1226, 1508
PspN4I	GGNNCC	5 cut(s)	6, 19, 555, 1169, 2290
PspPI	GGNCC	7 cut(s)	287, 692, 716, 1802, 2330, 2393, 2442
PspPPI	RGGWCCY	1 cut(s)	716
PspXI	VCTCGAGB	1 cut(s)	162
PstI	CTGCAG	2 cut(s)	1771, 1963
PstNI	CAGNNNCTG	1 cut(s)	2375
PsuI	RGATCY	1 cut(s)	2473
PvuI	CGATCG	1 cut(s)	148
RsaI	GTAC	4 cut(s)	374, 969, 1100, 1658
RsaNI	GTAC	4 cut(s)	373, 968, 1099, 1657
RseI	CAYNNNNRTG	6 cut(s)	637, 1023, 1050, 1760, 1869, 2407
SacI	GAGCTC	1 cut(s)	600
SapI	GCTCTTC	1 cut(s)	588
SaqAI	TTAA	8 cut(s)	336, 582, 972, 1079, 1364, 1427, 2192, 2294
SatI	GCNGC	8 cut(s)	122, 140, 208, 240, 780, 1043, 1224, 1767
Sau3AI	GATC	6 cut(s)	129, 145, 430, 434, 2473, 2500
Sau96I	GGNCC	7 cut(s)	287, 692, 716, 1802, 2330, 2393, 2442
SchI	GAGTC	5 cut(s)	192, 229, 419, 536, 1991
SduI	GDGCHC	5 cut(s)	93, 177, 600, 1170, 1924
SfaNI	GCATC	5 cut(s)	518, 1131, 1418, 1873, 1954
SfcI	CTRYAG	4 cut(s)	355, 1767, 1797, 1959
SfoI	GGCGCC	1 cut(s)	6
Sfr274I	CTCGAG	1 cut(s)	162
SfuI	TTCGAA	1 cut(s)	823
SinI	GGWCC	5 cut(s)	287, 692, 716, 2393, 2442
SlaI	CTCGAG	1 cut(s)	162
SmiMI	CAYNNNNRTG	6 cut(s)	637, 1023, 1050, 1760, 1869, 2407
SmlI	CTYRAG	2 cut(s)	162, 1401
SmoI	CTYRAG	2 cut(s)	162, 1401
SsiI	CCGC	7 cut(s)	122, 140, 208, 690, 1518, 1821, 2172
SspDI	GGCGCC	1 cut(s)	4
SspI	AATATT	3 cut(s)	391, 828, 2323
SspMI	CTAG	6 cut(s)	720, 1238, 1287, 1877, 2340, 2599
SstI	GAGCTC	1 cut(s)	600
StyI	CCWWGG	3 cut(s)	765, 877, 1175
TaaI	ACNGT	7 cut(s)	319, 913, 1492, 1542, 2242, 2280, 2465
TaiI	ACGT	3 cut(s)	180, 1060, 1622
TauI	GCSGC	3 cut(s)	124, 142, 210
TfiI	GAWTC	6 cut(s)	97, 150, 416, 1159, 2009, 2255
Tru1I	TTAA	8 cut(s)	336, 582, 972, 1079, 1364, 1427, 2192, 2294
Tru9I	TTAA	8 cut(s)	336, 582, 972, 1079, 1364, 1427, 2192, 2294
TscAI	CASTG	3 cut(s)	918, 1545, 1832
TseFI	GTSAC	3 cut(s)	845, 1783, 1823
TseI	GCWGC	5 cut(s)	239, 779, 1042, 1223, 1766
Tsp45I	GTSAC	3 cut(s)	845, 1783, 1823
TspGWI	ACGGA	1 cut(s)	1534
TspRI	CASTG	3 cut(s)	918, 1545, 1832
Van91I	CCANNNNNTGG	1 cut(s)	1693
VneI	GTGCAC	2 cut(s)	89, 1920
VpaK11BI	GGWCC	5 cut(s)	287, 692, 716, 2393, 2442
XceI	RCATGY	2 cut(s)	109, 2042
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1377
XhoI	CTCGAG	1 cut(s)	162
XmiI	GTMKAC	1 cut(s)	937
XmnI	GAANNNNTTC	1 cut(s)	829
XspI	CTAG	6 cut(s)	720, 1238, 1287, 1877, 2340, 2599

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.