AT1G69610

Protein of unknown function (DUF1666)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0014609
Physical Location & Seq
Forward (+)
26186713 .. 26189568
2856 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G69610.2

Sequence Viewer

Length: 1911 bp
ATGGGTATTGTTACGACGAAGACGAAGACTTATACAACTACTAACTTCGATTTCTCCAATAAAGGTTTTGGTGTTGGTGGTTTGAAACAGTTTGTGTACGGAAACACTTTCATTGTTCTCGATGCTTTCTGGGTTTCTTTCTCTAATCTCTTCAGAGTTAATCCAGACAAAGAGACAAGCTTGGTCTTGTTCGAGAAACAGAACACAAAAGATTCCTCTGTTTTTGGAGATCATCAAGATCAAAACCAAATTTGTGGAACACAAGACGATGATCAGATGGAAAGCTCTCTGGTTTCGATTAGCAAGAGATGCGAGTTTTATTCCGACAACAAAAGTATCGTTGGTTTTGTAGAAGAACCTAAAGCGTTTAGCTTTAAGTTTCATGAGTATTCTGCTATTACTGTTCAGGAGGAGGAGAAGAAGAAGATGGTGAGTTTCTCTGATGAAAAAACGAGTTCCATGGTTTTGGAGAAATCAGAATCAGATGGTTTTATGAAGGAACACGTGAAAGAAGAGATGTTGGTTTATGAGTTCATGAGTTGTGGTGTTTTAAAAGAGTCATTGGTTCACGAGAATCTTGTTGGTGGTGAAGACAACTTGTTTGATGATGATGATGGGTTTATAGAACTAAACCCAAGTTTACAGATTTCAAACTTTGCTTATGGAGAAGAAGAAGAAGAAGAGTTTATACACAGAGAAGAAGAGATGAAAATGGGCTTTGATGAACAAGAAGAGGTATATGATGAGTTTGATGATGGTTCAGATTCAGATGATGATGAGTTTGAGCATAGTGATGTGATTGAGAAGTTGAAAACAGAATTAAGGACTGCAAGGACAGGAGGATTGTGCACTATTCTTGAGGAATCAGAGACTCCATTACAAGAGCTGAAACCACTCAAGATTGAACCAAAACCAGATCAACACAAAGACAGAATTGCTGAAATTCACAAGGTTTACAAGAACTACGCGGTTAAAATGCGAAAACTCGATGTCATCGATTCTCAAACAATGCATTCCATCAGTTTGCTAAAGCTAAAAGATTCATCTAAACCTAGTAGAAACACTGATAAACCGCCTAAATCGTCTCTGCATCAGAATATATGGCCATTCAAGAAACATACGCTTGAATGTGACCCGAGTGAGAGACTTGTGAAAGAAGCAAGCAGAGACTTTGAGACTGTCTATGTAGGCCAAGTTTGTCTCTCGTGGGAGATGCTTCGATGGCAATACGACAAAGTCTTGGAATTTGATTCACAGGTTACTACTTATCAATACAACTTAGTAGCTGGAGAGTTTCAGTTATTCCAAGTTCTGCTTCAACGGTTTGTGGAGAATGAACCGTTTCAGAACTCCTCTAGAGTTGAGACTTACTTAAAGAACCGTCGCCATTTCCAGAATTTCCTTCAGATTCCACTTGTTAGAGATGACCGTTCTTCGAAGAAGAAATGTAGATATGAAGGAGAATTCGCGGTAAAGACTGAAATGTTGAGAGAGATTATCCGAGAGTCGATGAGTGTTTTCTGGGAGTTTCTTTGTGCAGACAAAGACGAGTTTACTTCAATGATGAAAGTTTCTCACCAAACTCAAGTCTCTCCACAGGATTCTTTAGACCTTGAGCTCTTGACAGATATCAGAACTCATCTCCAAAAGAAAGAGAAGAAGCTAAAGGAAATACAAAGAAGCCAAAGCTGTATAGTGAAGAAGTTAAAGAAGAATGAATCAAAGAGTAGTATTGGTGTGAAGGATGAATTACTAATAGCTAAAATTGAACTGAGACTGGTTTCAAGAGTGATGTACATGTCGAAACTTACTACTGAGAAGTTACATTGGTGTCAGGAGAAACTTGAAAAGATCAGCTTCAACGGTCGGAAGATTCATATGGAACCATATTTCTCGTTGTTACCTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1666

Protein Analysis

636

Amino Acids

74.32

Weight (kDa)

5.21

Isoelectric Point (pI)

47.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1666 PF07891 390 - 636 8.7e-88 Protein of unknown function (DUF1666)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014609)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G69610 AT1G69610 AT1G69610
fragaria_vesca FvH4_4g31360
malus_domestica MD16G1072900.v1.1
prunus_persica Prupe.1G274600_v2.0.a1
pyrus_communis pycom16g06320
rosa_chinensis RchiOBHm_Chr4g0439871
rosa_laevigata RLG00000006231
rosa_multiflora Rmu_sc0000497.1_g000007
rosa_roxburghii Rroxscaffold_5G00380780
rosa_rugosa Rorug04G0321800
rosa_samantha Rh4AG372900 Rh4BG385300 Rh4CG399800 Rh4DG379500
rosa_wichuraiana Rw4G031880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 968, 1469
AciI CCGC 3 cut(s) 968, 1073, 1469
AcoI YGGCCR 1 cut(s) 1103
AcsI RAATTY 5 cut(s) 249, 942, 1244, 1396, 1463
AcuI CTGAAG 2 cut(s) 136, 1388
AcvI CACGTG 1 cut(s) 505
AfaI GTAC 2 cut(s) 98, 1796
AflIII ACRYGT 2 cut(s) 502, 1797
AjuI GAANNNNNNNTTGG 2 cut(s) 1299, 1331
Alw21I GWGCWC 2 cut(s) 851, 1620
Alw44I GTGCAC 1 cut(s) 847
Ama87I CYCGRG 1 cut(s) 1135
AoxI GGCC 2 cut(s) 1103, 1189
ApaLI GTGCAC 1 cut(s) 847
ApoI RAATTY 5 cut(s) 249, 942, 1244, 1396, 1463
ArsI GACNNNNNNTTYG 2 cut(s) 584, 616
Asp700I GAANNNNTTC 1 cut(s) 1341
AsuHPI GGTGA 3 cut(s) 442, 599, 1568
AsuII TTCGAA 1 cut(s) 1436
AvaI CYCGRG 1 cut(s) 1135
BaeGI GKGCMC 1 cut(s) 851
BaeI ACNNNNGTAYC 2 cut(s) 319, 352
BalI TGGCCA 1 cut(s) 1105
BanII GRGCYC 1 cut(s) 1620
BarI GAAGNNNNNNTAC 2 cut(s) 672, 704
BauI CACGAG 2 cut(s) 569, 1204
BbrPI CACGTG 1 cut(s) 505
BbsI GAAGAC 3 cut(s) 26, 32, 597
Bbv12I GWGCWC 2 cut(s) 851, 1620
BccI CCATC 7 cut(s) 271, 421, 479, 608, 749, 1025, 1215
BclI TGATCA 1 cut(s) 271
BfaI CTAG 2 cut(s) 1053, 1356
BmeT110I CYCGRG 1 cut(s) 1135
BmiI GGNNCC 1 cut(s) 1884
BmsI GCATC 4 cut(s) 112, 299, 1099, 1203
BpiI GAAGAC 3 cut(s) 26, 32, 597
BpmI CTGGAG 1 cut(s) 1308
Bpu14I TTCGAA 1 cut(s) 1436
BpuEI CTTGAG 4 cut(s) 878, 881, 1569, 1634
Bsa29I ATCGAT 1 cut(s) 996
BsaAI YACGTR 1 cut(s) 505
BsaJI CCNNGG 1 cut(s) 459
Bse1I ACTGG 1 cut(s) 1782
BseCI ATCGAT 1 cut(s) 996
BseDI CCNNGG 1 cut(s) 459
BseGI GGATG 1 cut(s) 1750
BseMII CTCAG 2 cut(s) 1763, 1806
BseNI ACTGG 1 cut(s) 1782
BseRI GAGGAG 3 cut(s) 425, 428, 1342
BseSI GKGCMC 1 cut(s) 851
BsgI GTGCAG 1 cut(s) 1557
Bsh1236I CGCG 2 cut(s) 968, 1469
Bsh1285I CGRYCG 1 cut(s) 1867
BshFI GGCC 2 cut(s) 1105, 1191
BshVI ATCGAT 1 cut(s) 996
BsiEI CGRYCG 1 cut(s) 1867
BsiHKAI GWGCWC 2 cut(s) 851, 1620
BsiHKCI CYCGRG 1 cut(s) 1135
BsmBI CGTCTC 1 cut(s) 1089
BsmI GAATGC 1 cut(s) 1012
BsnI GGCC 2 cut(s) 1105, 1191
BsoBI CYCGRG 1 cut(s) 1135
Bsp119I TTCGAA 1 cut(s) 1436
Bsp1286I GDGCHC 2 cut(s) 851, 1620
Bsp1407I TGTACA 1 cut(s) 1794
Bsp143I GATC 5 cut(s) 229, 238, 271, 916, 1851
Bsp19I CCATGG 1 cut(s) 459
BspACI CCGC 3 cut(s) 968, 1073, 1469
BspANI GGCC 2 cut(s) 1105, 1191
BspCNI CTCAG 2 cut(s) 1764, 1807
BspDI ATCGAT 1 cut(s) 996
BspFNI CGCG 2 cut(s) 968, 1469
BspHI TCATGA 2 cut(s) 382, 534
BspLI GGNNCC 1 cut(s) 1884
BspT104I TTCGAA 1 cut(s) 1436
BsrGI TGTACA 1 cut(s) 1794
BsrI ACTGG 1 cut(s) 1782
BssECI CCNNGG 1 cut(s) 459
BssMI GATC 5 cut(s) 229, 238, 271, 916, 1851
BssSI CACGAG 2 cut(s) 569, 1204
BssT1I CCWWGG 1 cut(s) 459
Bst2BI CACGAG 2 cut(s) 569, 1204
Bst4CI ACNGT 8 cut(s) 90, 403, 1180, 1323, 1341, 1382, 1430, 1865
Bst6I CTCTTC 5 cut(s) 155, 507, 675, 696, 726
BstAPI GCANNNNNTGC 1 cut(s) 309
BstAUI TGTACA 1 cut(s) 1794
BstBAI YACGTR 1 cut(s) 505
BstBI TTCGAA 1 cut(s) 1436
BstC8I GCNNGC 1 cut(s) 1162
BstDEI CTNAG 3 cut(s) 1279, 1772, 1815
BstDSI CCRYGG 1 cut(s) 459
BstF5I GGATG 1 cut(s) 1750
BstFNI CGCG 2 cut(s) 968, 1469
BstKTI GATC 5 cut(s) 232, 241, 274, 919, 1854
BstMBI GATC 5 cut(s) 229, 238, 271, 916, 1851
BstMCI CGRYCG 1 cut(s) 1867
BstMWI GCNNNNNNNGC 2 cut(s) 309, 1222
BstNSI RCATGY 1 cut(s) 1801
BstSLI GKGCMC 1 cut(s) 851
BstUI CGCG 2 cut(s) 968, 1469
BstV2I GAAGAC 3 cut(s) 26, 32, 597
BstXI CCANNNNNNTGG 2 cut(s) 254, 466
Bsu15I ATCGAT 1 cut(s) 996
BsuRI GGCC 2 cut(s) 1105, 1191
BsuTUI ATCGAT 1 cut(s) 996
BtgI CCRYGG 1 cut(s) 459
BtsCI GGATG 1 cut(s) 1750
BtsIMutI CAGTG 1 cut(s) 1062
Cac8I GCNNGC 1 cut(s) 1162
CciI TCATGA 2 cut(s) 382, 534
ClaI ATCGAT 1 cut(s) 996
Csp6I GTAC 2 cut(s) 97, 1795
CviAII CATG 4 cut(s) 383, 460, 535, 1798
CviQI GTAC 2 cut(s) 97, 1795
DdeI CTNAG 3 cut(s) 1279, 1772, 1815
DpnI GATC 5 cut(s) 231, 240, 273, 918, 1853
DpnII GATC 5 cut(s) 229, 238, 271, 916, 1851
DraI TTTAAA 1 cut(s) 552
EaeI YGGCCR 1 cut(s) 1103
Eam1104I CTCTTC 5 cut(s) 155, 507, 675, 696, 726
EarI CTCTTC 5 cut(s) 155, 507, 675, 696, 726
Ecl136II GAGCTC 1 cut(s) 1618
Eco130I CCWWGG 1 cut(s) 459
Eco24I GRGCYC 1 cut(s) 1620
Eco32I GATATC 1 cut(s) 1630
Eco53kI GAGCTC 1 cut(s) 1618
Eco57I CTGAAG 2 cut(s) 136, 1388
Eco72I CACGTG 1 cut(s) 505
Eco88I CYCGRG 1 cut(s) 1135
EcoICRI GAGCTC 1 cut(s) 1618
EcoRI GAATTC 1 cut(s) 1463
EcoRV GATATC 1 cut(s) 1630
EcoT14I CCWWGG 1 cut(s) 459
EcoT22I ATGCAT 1 cut(s) 1014
EcoT38I GRGCYC 1 cut(s) 1620
ErhI CCWWGG 1 cut(s) 459
Esp3I CGTCTC 1 cut(s) 1089
FaeI CATG 4 cut(s) 386, 463, 538, 1801
FalI AAGNNNNNCTT 4 cut(s) 1299, 1331, 1841, 1873
FatI CATG 4 cut(s) 382, 459, 534, 1797
FauNDI CATATG 1 cut(s) 1878
FbaI TGATCA 1 cut(s) 271
FokI GGATG 1 cut(s) 1757
FriOI GRGCYC 1 cut(s) 1620
FspBI CTAG 2 cut(s) 1053, 1356
GsuI CTGGAG 1 cut(s) 1308
HaeIII GGCC 2 cut(s) 1105, 1191
Hin1II CATG 4 cut(s) 386, 463, 538, 1801
HindIII AAGCTT 1 cut(s) 178
HphI GGTGA 3 cut(s) 442, 599, 1568
Hpy166II GTNNAC 6 cut(s) 97, 568, 641, 849, 955, 1554
Hpy8I GTNNAC 6 cut(s) 97, 568, 641, 849, 955, 1554
Hpy99I CGWCG 2 cut(s) 19, 1386
HpyAV CCTTC 4 cut(s) 490, 1412, 1451, 1735
HpyCH4III ACNGT 8 cut(s) 90, 403, 1180, 1323, 1341, 1382, 1430, 1865
HpyCH4IV ACGT 1 cut(s) 504
HpyCH4V TGCA 5 cut(s) 830, 849, 1012, 1090, 1538
HpyF10VI GCNNNNNNNGC 2 cut(s) 309, 1222
HpyF3I CTNAG 3 cut(s) 1279, 1772, 1815
HpySE526I ACGT 1 cut(s) 504
Hsp92II CATG 4 cut(s) 386, 463, 538, 1801
Ksp22I TGATCA 1 cut(s) 271
Kzo9I GATC 5 cut(s) 229, 238, 271, 916, 1851
LweI GCATC 4 cut(s) 112, 299, 1099, 1203
MaeI CTAG 2 cut(s) 1053, 1356
MaeII ACGT 1 cut(s) 504
MaeIII GTNAC 5 cut(s) 10, 1130, 1258, 1821, 1899
MalI GATC 5 cut(s) 231, 240, 273, 918, 1853
MboI GATC 5 cut(s) 229, 238, 271, 916, 1851
MhlI GDGCHC 2 cut(s) 851, 1620
MlsI TGGCCA 1 cut(s) 1105
MluCI AATT 9 cut(s) 249, 818, 933, 942, 1244, 1396, 1463, 1748, 1764
MluNI TGGCCA 1 cut(s) 1105
MlyI GAGTC 3 cut(s) 566, 865, 1514
MmeI TCCRAC 2 cut(s) 348, 1847
MnlI CCTC 7 cut(s) 226, 403, 406, 727, 833, 853, 1363
Mox20I TGGCCA 1 cut(s) 1105
Mph1103I ATGCAT 1 cut(s) 1014
MroXI GAANNNNTTC 1 cut(s) 1341
MscI TGGCCA 1 cut(s) 1105
MseI TTAA 7 cut(s) 159, 375, 551, 821, 972, 1373, 1706
MslI CAYNNNNRTG 1 cut(s) 792
Msp20I TGGCCA 1 cut(s) 1105
Mva1269I GAATGC 1 cut(s) 1012
MvnI CGCG 2 cut(s) 968, 1469
MwoI GCNNNNNNNGC 2 cut(s) 309, 1222
NcoI CCATGG 1 cut(s) 459
NdeI CATATG 1 cut(s) 1878
NdeII GATC 5 cut(s) 229, 238, 271, 916, 1851
NlaIII CATG 4 cut(s) 386, 463, 538, 1801
NlaIV GGNNCC 1 cut(s) 1884
NmuCI GTSAC 1 cut(s) 1130
NsiI ATGCAT 1 cut(s) 1014
NspI RCATGY 1 cut(s) 1801
NspV TTCGAA 1 cut(s) 1436
PagI TCATGA 2 cut(s) 382, 534
PciI ACATGT 1 cut(s) 1797
PcsI WCGNNNNNNNCGW 2 cut(s) 20, 993
PctI GAATGC 1 cut(s) 1012
PdmI GAANNNNTTC 1 cut(s) 1341
PflFI GACNNNGTC 1 cut(s) 1235
PleI GAGTC 3 cut(s) 565, 865, 1513
PmaCI CACGTG 1 cut(s) 505
PmlI CACGTG 1 cut(s) 505
PpsI GAGTC 3 cut(s) 565, 865, 1513
Ppu21I YACGTR 1 cut(s) 505
PscI ACATGT 1 cut(s) 1797
Psp124BI GAGCTC 1 cut(s) 1620
PspCI CACGTG 1 cut(s) 505
PspN4I GGNNCC 1 cut(s) 1884
PsyI GACNNNGTC 1 cut(s) 1235
RsaI GTAC 2 cut(s) 98, 1796
RsaNI GTAC 2 cut(s) 97, 1795
RseI CAYNNNNRTG 1 cut(s) 792
SacI GAGCTC 1 cut(s) 1620
SaqAI TTAA 7 cut(s) 159, 375, 551, 821, 972, 1373, 1706
Sau3AI GATC 5 cut(s) 229, 238, 271, 916, 1851
SchI GAGTC 3 cut(s) 566, 865, 1514
SduI GDGCHC 2 cut(s) 851, 1620
SfaNI GCATC 4 cut(s) 112, 299, 1099, 1203
SfuI TTCGAA 1 cut(s) 1436
SmiMI CAYNNNNRTG 1 cut(s) 792
SmlI CTYRAG 4 cut(s) 857, 896, 1584, 1613
SmoI CTYRAG 4 cut(s) 857, 896, 1584, 1613
Sse9I AATT 9 cut(s) 249, 818, 933, 942, 1244, 1396, 1463, 1748, 1764
SsiI CCGC 3 cut(s) 968, 1073, 1469
SspMI CTAG 2 cut(s) 1053, 1356
SstI GAGCTC 1 cut(s) 1620
StyI CCWWGG 1 cut(s) 459
TaaI ACNGT 8 cut(s) 90, 403, 1180, 1323, 1341, 1382, 1430, 1865
TaiI ACGT 1 cut(s) 507
TasI AATT 9 cut(s) 249, 818, 933, 942, 1244, 1396, 1463, 1748, 1764
TatI WGTACW 1 cut(s) 1794
Tru1I TTAA 7 cut(s) 159, 375, 551, 821, 972, 1373, 1706
Tru9I TTAA 7 cut(s) 159, 375, 551, 821, 972, 1373, 1706
TscAI CASTG 1 cut(s) 1069
TseFI GTSAC 1 cut(s) 1130
Tsp45I GTSAC 1 cut(s) 1130
TspGWI ACGGA 1 cut(s) 114
TspRI CASTG 1 cut(s) 1069
Tth111I GACNNNGTC 1 cut(s) 1235
VneI GTGCAC 1 cut(s) 847
XapI RAATTY 5 cut(s) 249, 942, 1244, 1396, 1463
XbaI TCTAGA 1 cut(s) 1355
XceI RCATGY 1 cut(s) 1801
XmnI GAANNNNTTC 1 cut(s) 1341
XspI CTAG 2 cut(s) 1053, 1356
Zsp2I ATGCAT 1 cut(s) 1014
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.