FvH4_4g31360

Protein of unknown function (DUF1666)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0014609
Physical Location & Seq
Reverse (-)
30542974 .. 30547893
4920 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g31360.t1

Sequence Viewer

Length: 2373 bp
ATGATGGGTATGAAGAAATTCATGGTGCTGAAAACCCAGTTTGATTATATTAGCAAACGCCTTTCAAGCGGAAACATGCTTGGAGTGATTGGTTCTATCTGGGTTTTTGTCTGCAACTACTTGTTCTTTGTGTCTGGATTCATTAGTAGATACATACTCATCAGATTTCAGGAACATGATCACAGAAATCATCACCCTGTTGTTTGTTTGGAGCATGATCATCAATTTGATGCAAATAGAGAAGAAGAAGAAGACTTGTCTTTGGATTCTCCTAGAAGAATGAATGATGATCAAAACAGAGCGGAAACAGAGGATTCCGATCAAAACAGAGCGGAAACAGAGGATTCGGATCAAAACAGAGCAGAAACAGAGGATTCCCGTCTTGTGAAGACTGTCTCAGCTGTAAGCACCGATTTATATGAGTATACTTCTAACAGAGCTATCAATGGCTACATGGAAGAACCAAAATCCATGAGCTTTACTGTGCAAGAATTCTATGTTGATTGGGGCAGTTCTAAATTCTCTGATTATCAAATTCCTATTAAAGGCGCAGAGGATGATGATGCTGATCATGAAAAAGCAGTGGAGTCTATTGAAAGCGTTGAGGCCTCAGAGAATGAAGCGTACAAGAATCCAGTCACAGATCAAAACCCTATCGAGGAATTTAGTACCAGGAAGGTTTTAGATGCTGAAATTGAGGTTGAAGAAGATAATTGCTCTGTAGTTTCTGATTCGAAACAAGACCCAGTTAGCTCAAGAAAAGATCAAGACGTGGGTTCACTTAACTATCAGTTCTTGATATACAGAAATGTGGTTCAGTATGATGAGGGACTTGAACCTCAAAGCTCTGGGTTTCAGTATGACGAGGAAGATGATCATAGAGTCCACTTTGATATGGAAGATGATCAATTGCTTGATGTAAGTATCCCAGATGGAAAAGCAGTTGTTTTTTCCGATCAGCGCGATGGTTCCGGATCTTCTGATGATGAATACATTGAATTGTTAGAGCCCCAGTTGAGAAGTTATAGTCAACAACTTGATCAAGTTGAGGAGAAAGGTGAACATAAAGAAGAATTAGTCCAAGTTGAAACACAAGGCACTAGTCCACTAGTGCAATCTGCAGAAACACAAGGCACTAGTCCACTAGTGCAACCTGCAGTAGAAGAAAATGTGTTGCAGCAAACAAAGTCTGGATTGGTTTCGGATGATGAAAACGATTTGGAGTCTTATGAGGAGCATGATCTAATTGAACAGATTAAAAGGGAAATTAAGAATGTGAGAACAGGAGGGCTTCCCACTATTTCAGAAGAAGATCATTCAGAATCTACAAAAATGGTGGAGAATCTAAAGCTGAAGCCACTGAAGATTGATGAAAAACTTGAATACAAAGATCGAATCGCAGAGATTCAGAAGGTCTACAAAAGCTACGCCGAGAAAATGCGAAAACTGGACATCTTGAACAACCAGACCATGCATGCAATCGGTTTTCTGCAGCTGAAAGACCAAGTTAAGCCAATTTCATTGCAGAAGTCTTCCATTAATCCAATCATGAAGTCTCTTCTTTCACAAAATATGTTGAGATGTAGAGCCCAAAGACGACCCACAGTTGACCCTTTGCCGAAATTTGTAGGAGACTTGCACAAGGACTTGGAGTTGGTGTATGTTGGACAGGTTTGCCTCTCTTGGGAAATTCTCCACTGGCAGCACAGAAAAGCCCAAGAGCTGCGGCAATATGATCATCATCACTATAATGCTGTTGCCAATGAGTTTCAACTGTTTCAAGTGCTTCTGCAAAGATTCATAGAAGACGATCCATTTCAAGGCCCCCGAGTGCAGCACTATGTCAGGAACCGATGTGTCCTTCGTAGTCTTCTTCAAGTTCCACCCATAAGAGATGATTGTAGGAAGGATAAGAAACATGGAATGGGAGGGGAGGAGGAGGATTTGATAATCTTAAGTGAAATGCTGGTGAAAATCATTGAAGAAACGATGCAGATATATTGGAATTTTCTTCGCAGTGATGAGAAAAATGTGGTCTTGAAGACCTTTCAAGAAAGCCAGGTTGAGCATAAGGATTTGGAGCTGCTTATAGACATCCGGAGAGATCTTCAAAAGAAGGAGAAAAAACTCAGAGACATACAGAGAATCGGAAATTGTGTAGTGAGGAAGTTCCAAAAGCAGCTTCAAGATCAAGACAGACTTGATCATTGTTTGTTTGTGGCTCAAGTTGAACTGAGGTTGGTATTGAGAGTGCTGAATATGTCGAAACTAACATCAGATCAGTTAGTTTGGTGCCATGAGAAGCTAGACAACATCAATTTTGTTCACAGAAAGGTTCTTATGGAGCCTTCGTTTCTTCTTTTTCCATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1666

Protein Analysis

791

Amino Acids

92.16

Weight (kDa)

4.91

Isoelectric Point (pI)

52.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1666 PF07891 548 - 789 7.3e-87 Protein of unknown function (DUF1666)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014609)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G69610 AT1G69610 AT1G69610
fragaria_vesca FvH4_4g31360
malus_domestica MD16G1072900.v1.1
prunus_persica Prupe.1G274600_v2.0.a1
pyrus_communis pycom16g06320
rosa_chinensis RchiOBHm_Chr4g0439871
rosa_laevigata RLG00000006231
rosa_multiflora Rmu_sc0000497.1_g000007
rosa_roxburghii Rroxscaffold_5G00380780
rosa_rugosa Rorug04G0321800
rosa_samantha Rh4AG372900 Rh4BG385300 Rh4CG399800 Rh4DG379500
rosa_wichuraiana Rw4G031880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1162
AccB1I GGYRCC 1 cut(s) 2292
AccBSI CCGCTC 2 cut(s) 302, 332
AccI GTMKAC 2 cut(s) 425, 1416
AccII CGCG 1 cut(s) 963
AccIII TCCGGA 2 cut(s) 971, 2097
AciI CCGC 4 cut(s) 69, 302, 332, 1726
AclWI GGATC 3 cut(s) 357, 982, 1805
AcsI RAATTY 8 cut(s) 17, 491, 518, 534, 662, 1622, 1689, 2005
AcuI CTGAAG 2 cut(s) 1373, 1382
AfaI GTAC 2 cut(s) 626, 670
AfiI CCNNNNNNNGG 4 cut(s) 545, 658, 1684, 1820
AflII CTTAAG 1 cut(s) 1954
AhlI ACTAGT 4 cut(s) 1100, 1108, 1136, 1144
AjiI CACGTC 1 cut(s) 772
AjnI CCWGG 2 cut(s) 671, 2058
AjuI GAANNNNNNNTTGG 2 cut(s) 2166, 2198
Alw26I GTCTC 4 cut(s) 400, 1560, 1626, 2127
AlwI GGATC 3 cut(s) 357, 982, 1805
Ama87I CYCGRG 1 cut(s) 1827
Aor13HI TCCGGA 2 cut(s) 971, 2097
AoxI GGCC 2 cut(s) 606, 1822
ApeKI GCWGC 7 cut(s) 1177, 1492, 1702, 1723, 1834, 2083, 2179
ApoI RAATTY 8 cut(s) 17, 491, 518, 534, 662, 1622, 1689, 2005
AseI ATTAAT 1 cut(s) 1539
Asp700I GAANNNNTTC 1 cut(s) 17
AspLEI GCGC 2 cut(s) 551, 963
AspS9I GGNCC 1 cut(s) 1823
AsuHPI GGTGA 3 cut(s) 185, 1070, 1981
AsuII TTCGAA 1 cut(s) 734
AvaI CYCGRG 1 cut(s) 1827
BanI GGYRCC 1 cut(s) 2292
BanII GRGCYC 2 cut(s) 1011, 1591
BbsI GAAGAC 6 cut(s) 258, 395, 1524, 1812, 1862, 2048
BbvI GCAGC 7 cut(s) 1189, 1504, 1710, 1714, 1846, 2070, 2191
BccI CCATC 2 cut(s) 926, 959
BciT130I CCWGG 2 cut(s) 673, 2060
BciVI GTATCC 1 cut(s) 935
BclI TGATCA 9 cut(s) 178, 217, 289, 568, 874, 904, 1039, 1735, 2203
BcoDI GTCTC 4 cut(s) 400, 1560, 1626, 2127
BcuI ACTAGT 4 cut(s) 1100, 1108, 1136, 1144
BfaI CTAG 6 cut(s) 273, 1101, 1109, 1137, 1145, 2306
BfmI CTRYAG 4 cut(s) 720, 1119, 1155, 1490
BfrI CTTAAG 1 cut(s) 1954
BfuAI ACCTGC 1 cut(s) 1162
BfuI GTATCC 1 cut(s) 935
BglII AGATCT 1 cut(s) 2104
BisI GCNGC 8 cut(s) 1178, 1493, 1703, 1724, 1727, 1835, 2084, 2180
BlsI GCNGC 8 cut(s) 1179, 1494, 1704, 1725, 1728, 1836, 2085, 2181
Bme1390I CCNGG 2 cut(s) 673, 2060
BmeT110I CYCGRG 1 cut(s) 1827
BmgBI CACGTC 1 cut(s) 772
BmgT120I GGNCC 1 cut(s) 1823
BmiI GGNNCC 5 cut(s) 970, 1825, 1850, 2294, 2346
BmrFI CCNGG 2 cut(s) 673, 2060
BmrI ACTGGG 3 cut(s) 31, 740, 1006
BmsI GCATC 4 cut(s) 220, 553, 676, 1980
BmuI ACTGGG 3 cut(s) 31, 740, 1006
BpiI GAAGAC 6 cut(s) 258, 395, 1524, 1812, 1862, 2048
Bpu14I TTCGAA 1 cut(s) 734
BpuEI CTTGAG 2 cut(s) 739, 2208
BsaBI GATNNNNATC 4 cut(s) 318, 348, 567, 1740
BsaWI WCCGGW 2 cut(s) 971, 2097
BsaXI ACNNNNNCTCC 4 cut(s) 1643, 1673, 2336, 2366
Bsc4I CCNNNNNNNGG 4 cut(s) 545, 658, 1684, 1820
Bse1I ACTGG 6 cut(s) 37, 635, 746, 1012, 1452, 1703
Bse3DI GCAATG 1 cut(s) 1520
Bse8I GATNNNNATC 4 cut(s) 318, 348, 567, 1740
BseAI TCCGGA 2 cut(s) 971, 2097
BseBI CCWGG 2 cut(s) 673, 2060
BseGI GGATG 3 cut(s) 562, 1210, 2094
BseJI GATNNNNATC 4 cut(s) 318, 348, 567, 1740
BseLI CCNNNNNNNGG 4 cut(s) 545, 658, 1684, 1820
BseMI GCAATG 1 cut(s) 1520
BseMII CTCAG 4 cut(s) 411, 624, 2143, 2225
BseNI ACTGG 6 cut(s) 37, 635, 746, 1012, 1452, 1703
BseRI GAGGAG 4 cut(s) 1064, 1247, 1949, 1952
BseXI GCAGC 7 cut(s) 1189, 1504, 1710, 1714, 1846, 2070, 2191
BsgI GTGCAG 1 cut(s) 1853
Bsh1236I CGCG 1 cut(s) 963
BshFI GGCC 2 cut(s) 608, 1824
BshNI GGYRCC 1 cut(s) 2292
BsiHKCI CYCGRG 1 cut(s) 1827
BsiSI CCGG 2 cut(s) 972, 2098
BslFI GGGAC 1 cut(s) 843
BslI CCNNNNNNNGG 4 cut(s) 545, 658, 1684, 1820
BsmAI GTCTC 4 cut(s) 400, 1560, 1626, 2127
BsmFI GGGAC 1 cut(s) 843
BsnI GGCC 2 cut(s) 608, 1824
BsoBI CYCGRG 1 cut(s) 1827
Bsp119I TTCGAA 1 cut(s) 734
Bsp1286I GDGCHC 2 cut(s) 1011, 1591
Bsp13I TCCGGA 2 cut(s) 971, 2097
BspACI CCGC 4 cut(s) 69, 302, 332, 1726
BspANI GGCC 2 cut(s) 608, 1824
BspCNI CTCAG 4 cut(s) 410, 623, 2142, 2226
BspEI TCCGGA 2 cut(s) 971, 2097
BspFNI CGCG 1 cut(s) 963
BspHI TCATGA 2 cut(s) 571, 1548
BspLI GGNNCC 5 cut(s) 970, 1825, 1850, 2294, 2346
BspMAI CTGCAG 3 cut(s) 1123, 1159, 1494
BspMI ACCTGC 1 cut(s) 1162
BspPI GGATC 3 cut(s) 357, 982, 1805
BspT104I TTCGAA 1 cut(s) 734
BspT107I GGYRCC 1 cut(s) 2292
BspTI CTTAAG 1 cut(s) 1954
BsrBI CCGCTC 2 cut(s) 302, 332
BsrDI GCAATG 1 cut(s) 1520
BsrI ACTGG 6 cut(s) 37, 635, 746, 1012, 1452, 1703
BssNAI GTATAC 1 cut(s) 426
Bst1107I GTATAC 1 cut(s) 426
Bst2UI CCWGG 2 cut(s) 673, 2060
Bst4CI ACNGT 4 cut(s) 394, 484, 1606, 1776
Bst6I CTCTTC 1 cut(s) 1563
BstAFI CTTAAG 1 cut(s) 1954
BstBI TTCGAA 1 cut(s) 734
BstC8I GCNNGC 1 cut(s) 1476
BstDEI CTNAG 4 cut(s) 397, 610, 2129, 2234
BstENI CCTNNNNNAGG 1 cut(s) 543
BstF5I GGATG 3 cut(s) 562, 1210, 2094
BstFNI CGCG 1 cut(s) 963
BstHHI GCGC 2 cut(s) 551, 963
BstMAI GTCTC 4 cut(s) 400, 1560, 1626, 2127
BstMWI GCNNNNNNNGC 1 cut(s) 66
BstNI CCWGG 2 cut(s) 673, 2060
BstNSI RCATGY 2 cut(s) 79, 1478
BstSCI CCNGG 2 cut(s) 671, 2058
BstSFI CTRYAG 4 cut(s) 720, 1119, 1155, 1490
BstUI CGCG 1 cut(s) 963
BstV1I GCAGC 7 cut(s) 1189, 1504, 1710, 1714, 1846, 2070, 2191
BstV2I GAAGAC 6 cut(s) 258, 395, 1524, 1812, 1862, 2048
BstX2I RGATCY 2 cut(s) 974, 2104
BstYI RGATCY 2 cut(s) 974, 2104
BstZ17I GTATAC 1 cut(s) 426
BsuI GTATCC 1 cut(s) 935
BsuRI GGCC 2 cut(s) 608, 1824
BtgZI GCGATG 1 cut(s) 978
BtrI CACGTC 1 cut(s) 772
BtsCI GGATG 3 cut(s) 562, 1210, 2094
BtsI GCAGTG 2 cut(s) 588, 2023
BtsIMutI CAGTG 4 cut(s) 588, 1358, 1696, 2023
BveI ACCTGC 1 cut(s) 1162
Cac8I GCNNGC 1 cut(s) 1476
CciI TCATGA 2 cut(s) 571, 1548
CfoI GCGC 2 cut(s) 551, 963
Cfr13I GGNCC 1 cut(s) 1823
Csp6I GTAC 2 cut(s) 625, 669
CspCI CAANNNNNGTGG 2 cut(s) 1317, 1352
CviQI GTAC 2 cut(s) 625, 669
DdeI CTNAG 4 cut(s) 397, 610, 2129, 2234
Eam1104I CTCTTC 1 cut(s) 1563
EarI CTCTTC 1 cut(s) 1563
Eco147I AGGCCT 1 cut(s) 608
Eco24I GRGCYC 2 cut(s) 1011, 1591
Eco57I CTGAAG 2 cut(s) 1373, 1382
Eco88I CYCGRG 1 cut(s) 1827
EcoNI CCTNNNNNAGG 1 cut(s) 543
EcoO109I RGGNCCY 1 cut(s) 1823
EcoRI GAATTC 1 cut(s) 491
EcoRII CCWGG 2 cut(s) 671, 2058
EcoT22I ATGCAT 1 cut(s) 1476
EcoT38I GRGCYC 2 cut(s) 1011, 1591
FalI AAGNNNNNCTT 2 cut(s) 2184, 2216
FaqI GGGAC 1 cut(s) 843
FbaI TGATCA 9 cut(s) 178, 217, 289, 568, 874, 904, 1039, 1735, 2203
FblI GTMKAC 2 cut(s) 425, 1416
Fnu4HI GCNGC 8 cut(s) 1178, 1493, 1703, 1724, 1727, 1835, 2084, 2180
FokI GGATG 3 cut(s) 569, 1217, 2081
FriOI GRGCYC 2 cut(s) 1011, 1591
Fsp4HI GCNGC 8 cut(s) 1178, 1493, 1703, 1724, 1727, 1835, 2084, 2180
FspBI CTAG 6 cut(s) 273, 1101, 1109, 1137, 1145, 2306
GlaI GCGC 2 cut(s) 550, 962
GluI GCNGC 8 cut(s) 1178, 1493, 1703, 1724, 1727, 1835, 2084, 2180
HaeIII GGCC 2 cut(s) 608, 1824
HapII CCGG 2 cut(s) 972, 2098
HhaI GCGC 2 cut(s) 551, 963
Hin6I GCGC 2 cut(s) 549, 961
HinP1I GCGC 2 cut(s) 549, 961
HincII GTYRAC 2 cut(s) 1031, 1609
HindII GTYRAC 2 cut(s) 1031, 1609
HpaII CCGG 2 cut(s) 972, 2098
HphI GGTGA 3 cut(s) 185, 1070, 1981
HpyAV CCTTC 6 cut(s) 670, 1405, 1871, 1900, 2110, 2358
HpyCH4III ACNGT 4 cut(s) 394, 484, 1606, 1776
HpyCH4IV ACGT 1 cut(s) 771
HpyF10VI GCNNNNNNNGC 1 cut(s) 66
HpyF3I CTNAG 4 cut(s) 397, 610, 2129, 2234
HpySE526I ACGT 1 cut(s) 771
HspAI GCGC 2 cut(s) 549, 961
Kpn2I TCCGGA 2 cut(s) 971, 2097
Ksp22I TGATCA 9 cut(s) 178, 217, 289, 568, 874, 904, 1039, 1735, 2203
LmnI GCTCC 4 cut(s) 211, 1234, 2080, 2344
Lsp1109I GCAGC 7 cut(s) 1189, 1504, 1710, 1714, 1846, 2070, 2191
LweI GCATC 4 cut(s) 220, 553, 676, 1980
MaeI CTAG 6 cut(s) 273, 1101, 1109, 1137, 1145, 2306
MaeII ACGT 1 cut(s) 771
MaeIII GTNAC 1 cut(s) 637
MbiI CCGCTC 2 cut(s) 302, 332
MfeI CAATTG 1 cut(s) 908
MflI RGATCY 2 cut(s) 974, 2104
MhlI GDGCHC 2 cut(s) 1011, 1591
MlyI GAGTC 3 cut(s) 596, 891, 1232
MmeI TCCRAC 1 cut(s) 1645
Mph1103I ATGCAT 1 cut(s) 1476
MroI TCCGGA 2 cut(s) 971, 2097
MroXI GAANNNNTTC 1 cut(s) 17
MseI TTAA 8 cut(s) 543, 783, 1257, 1269, 1509, 1539, 1955, 2371
MslI CAYNNNNRTG 1 cut(s) 1749
MspA1I CMGCKG 2 cut(s) 401, 1495
MspCI CTTAAG 1 cut(s) 1954
MspI CCGG 2 cut(s) 972, 2098
MspR9I CCNGG 2 cut(s) 673, 2060
MunI CAATTG 1 cut(s) 908
MvaI CCWGG 2 cut(s) 673, 2060
MvnI CGCG 1 cut(s) 963
MwoI GCNNNNNNNGC 1 cut(s) 66
NlaIV GGNNCC 5 cut(s) 970, 1825, 1850, 2294, 2346
NmeAIII GCCGAG 1 cut(s) 1456
NmuCI GTSAC 1 cut(s) 637
NsiI ATGCAT 1 cut(s) 1476
NspI RCATGY 2 cut(s) 79, 1478
NspV TTCGAA 1 cut(s) 734
PaeI GCATGC 1 cut(s) 1478
PagI TCATGA 2 cut(s) 571, 1548
PceI AGGCCT 1 cut(s) 608
PdmI GAANNNNTTC 1 cut(s) 17
PkrI GCNGC 8 cut(s) 1179, 1494, 1704, 1725, 1728, 1836, 2085, 2181
PleI GAGTC 3 cut(s) 595, 890, 1231
PpsI GAGTC 3 cut(s) 595, 890, 1231
PshBI ATTAAT 1 cut(s) 1539
Psp6I CCWGG 2 cut(s) 671, 2058
PspGI CCWGG 2 cut(s) 671, 2058
PspN4I GGNNCC 5 cut(s) 970, 1825, 1850, 2294, 2346
PspPI GGNCC 1 cut(s) 1823
PstI CTGCAG 3 cut(s) 1123, 1159, 1494
PsuI RGATCY 2 cut(s) 974, 2104
PvuII CAGCTG 2 cut(s) 401, 1495
RsaI GTAC 2 cut(s) 626, 670
RsaNI GTAC 2 cut(s) 625, 669
RseI CAYNNNNRTG 1 cut(s) 1749
SaqAI TTAA 8 cut(s) 543, 783, 1257, 1269, 1509, 1539, 1955, 2371
SatI GCNGC 8 cut(s) 1178, 1493, 1703, 1724, 1727, 1835, 2084, 2180
Sau96I GGNCC 1 cut(s) 1823
SchI GAGTC 3 cut(s) 596, 891, 1232
ScrFI CCNGG 2 cut(s) 673, 2060
SduI GDGCHC 2 cut(s) 1011, 1591
SfaNI GCATC 4 cut(s) 220, 553, 676, 1980
SfcI CTRYAG 4 cut(s) 720, 1119, 1155, 1490
SfuI TTCGAA 1 cut(s) 734
SmiMI CAYNNNNRTG 1 cut(s) 1749
SmlI CTYRAG 3 cut(s) 754, 1954, 2223
SmoI CTYRAG 3 cut(s) 754, 1954, 2223
SpeI ACTAGT 4 cut(s) 1100, 1108, 1136, 1144
SphI GCATGC 1 cut(s) 1478
SseBI AGGCCT 1 cut(s) 608
SsiI CCGC 4 cut(s) 69, 302, 332, 1726
SspMI CTAG 6 cut(s) 273, 1101, 1109, 1137, 1145, 2306
StuI AGGCCT 1 cut(s) 608
StyD4I CCNGG 2 cut(s) 671, 2058
TaaI ACNGT 4 cut(s) 394, 484, 1606, 1776
TaiI ACGT 1 cut(s) 774
TaqI TCGA 4 cut(s) 657, 734, 1393, 2264
TauI GCSGC 1 cut(s) 1729
Tru1I TTAA 8 cut(s) 543, 783, 1257, 1269, 1509, 1539, 1955, 2371
Tru9I TTAA 8 cut(s) 543, 783, 1257, 1269, 1509, 1539, 1955, 2371
TscAI CASTG 4 cut(s) 588, 1365, 1703, 2023
TseFI GTSAC 1 cut(s) 637
TseI GCWGC 7 cut(s) 1177, 1492, 1702, 1723, 1834, 2083, 2179
Tsp45I GTSAC 1 cut(s) 637
TspRI CASTG 4 cut(s) 588, 1365, 1703, 2023
Vha464I CTTAAG 1 cut(s) 1954
VspI ATTAAT 1 cut(s) 1539
XagI CCTNNNNNAGG 1 cut(s) 543
XapI RAATTY 8 cut(s) 17, 491, 518, 534, 662, 1622, 1689, 2005
XceI RCATGY 2 cut(s) 79, 1478
XmiI GTMKAC 2 cut(s) 425, 1416
XmnI GAANNNNTTC 1 cut(s) 17
XspI CTAG 6 cut(s) 273, 1101, 1109, 1137, 1145, 2306
Zsp2I ATGCAT 1 cut(s) 1476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.