Rh4DG379500

Protein of unknown function (DUF1666)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0014609
Physical Location & Seq
Reverse (-)
59753335 .. 59757487
4153 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG379500.1

Sequence Viewer

Length: 1890 bp
ATGGAAGAACCAAAAGCCACGAGCTTTATTGTCCAAGAATTCTATGTTGATTGGGGCAGTTCTAAATTCTCTGATTATCAAATTCCTATTAAAGCAGCAGAGGATGATGATGCTGATCAGGAAAAAGTAGTGGATTCTATTGAAAGCGTTGAGGCCTTGGAGAATGGAGAGCACGAGAAACCAGTCACTGATCAAAACCCTCTTGAGGAATTCAGTACTGGGAACGTTTTAGATGCTGAAATTGAGCTTTGTGAGGTTGAAGAAGACAATTTCTCTGTAGTTTCTGATTCGAAACAAGACCCGGTTAGCTCAAGAAAAGATCATGATGTGGGTTCACTTAACTACGAGTTTTTGATATACAGAAATGTGGTTCAGTTTGATGAGGGACTTGACCCTCAAAGCTCTGGATTCCAGTATGATGAGGAAGATCATAGAGTCCACTTTGATATGGAAGATGATCAGTTGCTTGATGTAAGTATCCCAGACGGAAAAGCAGTTGTCTTTTCCGATCAACTCGATGGTTCTGAATCTCCTGATGATGAATACATTGAATTGTTAGAGCCCCAATTGAGAAGTTATAGTCAACGACTTGATCAAGTTGTGGAGAGAGGTGAAGAAAAAGAGGAACTAGTCCAAGTTGAGACAAAAGCCACGAGTCCACTAGTACAATCTGCACAAGAAGAAATAGGATTGCAGCCTAAAAAGTTTGGATTGTATTCGGATGATGAAAACGAATTGGAGTCTCCTGACGAGCATGATGATCTAATTGAACAGATTAAAAGAGAAATTAAGAATGTGAGAACCGGAGGGCTTCCCACTATTTCAGAAGAAGATCATTCGGAGTCTACAAGAATGGTGGAGAATCTAAAGCTTAAGCCACTGAAGATTGATGAAAAGCTCGAATACAAAGATCGAATCGCAGAGATTCAGAAGGTGTACAAAAGCTACTCAGAGAAAATGCGAAAGCTGGACATTTTGAACAACCAGACCATGCATGCAATTGGTTTTCTACAGCTGAAAGACCAAGATAAGCCAATTTCATTGGAAAAGTCTTCCATTAATCCAATTATGAAGTCTCTTCTTTCGCAGAACCTGTTGAGGTGTAAAGCACAAAGACGACCGACAGTTGACCCTATGCCGAAATTTGTGGGAGACTTGCACAAGGATTTGGAATTGGTCTATGTTGGACAGGTTTGCCTCTCTTGGGAAATTCTTCACTGGCAGCACAGAAAAGCCCAAGAGTTGCAGCAATATGATCATCATCACTATAATGTTGTTGCCACTGAGTTTCAACTGTTTCAAGTGCTCCTGCAAAGATTCATAGAAGACGATCCATTTCAAGGCCCTCGAGTCCAGCACTATGTCCGGAACCGATGTGTCCTTCGTAGTCTTCTTCAAGTTCCAGCCATCAGAGATGATTGCAGGAAGGATAAGAAGCATGACATGGGAGGGGAGGAGGATTTGATAATCTCAAGTGAGATGCTGGTGAAAATCATTGAAGAAACGATGCAGATATTTTGGAAATTTCTTCGCAAGGATGAGAGAAATGCGGTCCTAAAGAGCCCTCAAGAAAGTCAGGTTGAGCATAAGGACTTGGAGCTTCTTGTAGATATCCGGAGGGATCTTCAAAAGAAGGAGAAAAAACTCAAAGACATACAGAGGAGTGGAAATTGTGTGGTGAAGAAGTTCCAAAAGCAGCATCAAGATCAGGACAGACTCGAGCATAGTTTTTTTGTCGCTCAAGTTGAACTGAGGTTGGTTTCAAGAGTGCTCAACATGTCAAAACTAACATCAGATCAGTTAGTTTGGTGCCATGAAAAGCTAGACAACATCAATTTTGTTCACAGAAGGGTTCTTATGGAGCCTTCGTTTCTTCTTTTTCCATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1666

Protein Analysis

629

Amino Acids

73.46

Weight (kDa)

4.85

Isoelectric Point (pI)

51.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1666 PF07891 388 - 628 2e-89 Protein of unknown function (DUF1666)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014609)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G69610 AT1G69610 AT1G69610
fragaria_vesca FvH4_4g31360
malus_domestica MD16G1072900.v1.1
prunus_persica Prupe.1G274600_v2.0.a1
pyrus_communis pycom16g06320
rosa_chinensis RchiOBHm_Chr4g0439871
rosa_laevigata RLG00000006231
rosa_multiflora Rmu_sc0000497.1_g000007
rosa_roxburghii Rroxscaffold_5G00380780
rosa_rugosa Rorug04G0321800
rosa_samantha Rh4AG372900 Rh4BG385300 Rh4CG399800 Rh4DG379500
rosa_wichuraiana Rw4G031880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1809
AccI GTMKAC 1 cut(s) 845
AccIII TCCGGA 2 cut(s) 1365, 1614
AciI CCGC 1 cut(s) 1550
AclI AACGTT 1 cut(s) 225
AclWI GGATC 2 cut(s) 1325, 1629
AcsI RAATTY 7 cut(s) 38, 65, 81, 209, 1142, 1209, 1523
AcuI CTGAAG 1 cut(s) 902
AfaI GTAC 3 cut(s) 217, 666, 938
AfiI CCNNNNNNNGG 3 cut(s) 205, 1204, 1340
AflII CTTAAG 1 cut(s) 872
AflIII ACRYGT 1 cut(s) 1776
AhlI ACTAGT 2 cut(s) 628, 661
Alw21I GWGCWC 3 cut(s) 174, 1308, 1773
Alw26I GTCTC 4 cut(s) 635, 747, 1080, 1146
AlwI GGATC 2 cut(s) 1325, 1629
AlwNI CAGNNNCTG 2 cut(s) 188, 1093
Ama87I CYCGRG 2 cut(s) 1347, 1718
Aor13HI TCCGGA 2 cut(s) 1365, 1614
AoxI GGCC 2 cut(s) 153, 1342
ApeKI GCWGC 5 cut(s) 95, 694, 1222, 1246, 1696
ApoI RAATTY 7 cut(s) 38, 65, 81, 209, 1142, 1209, 1523
AseI ATTAAT 1 cut(s) 1059
Asp700I GAANNNNTTC 2 cut(s) 1212, 1685
AspS9I GGNCC 2 cut(s) 1343, 1552
AsuC2I CCSGG 1 cut(s) 302
AsuHPI GGTGA 3 cut(s) 623, 1498, 1690
AsuII TTCGAA 1 cut(s) 290
AvaI CYCGRG 2 cut(s) 1347, 1718
AvaII GGWCC 1 cut(s) 1552
BanI GGYRCC 1 cut(s) 1809
BanII GRGCYC 2 cut(s) 564, 1565
BauI CACGAG 3 cut(s) 19, 173, 652
BbsI GAAGAC 4 cut(s) 270, 1044, 1332, 1382
Bbv12I GWGCWC 3 cut(s) 174, 1308, 1773
BbvI GCAGC 5 cut(s) 107, 706, 1234, 1258, 1708
BccI CCATC 2 cut(s) 512, 1415
BciVI GTATCC 1 cut(s) 488
BclI TGATCA 5 cut(s) 115, 190, 457, 592, 1255
BcnI CCSGG 1 cut(s) 302
BcoDI GTCTC 4 cut(s) 635, 747, 1080, 1146
BcuI ACTAGT 2 cut(s) 628, 661
BfaI CTAG 3 cut(s) 629, 662, 1823
BfmI CTRYAG 2 cut(s) 276, 1010
BfrI CTTAAG 1 cut(s) 872
BfuI GTATCC 1 cut(s) 488
BisI GCNGC 5 cut(s) 96, 695, 1223, 1247, 1697
BlsI GCNGC 5 cut(s) 97, 696, 1224, 1248, 1698
BmcAI AGTACT 1 cut(s) 217
Bme1390I CCNGG 1 cut(s) 302
Bme18I GGWCC 1 cut(s) 1552
BmeT110I CYCGRG 2 cut(s) 1347, 1718
BmgT120I GGNCC 2 cut(s) 1343, 1552
BmiI GGNNCC 3 cut(s) 1370, 1811, 1863
BmrFI CCNGG 1 cut(s) 302
BmrI ACTGGG 1 cut(s) 228
BmsI GCATC 5 cut(s) 100, 223, 1470, 1497, 1708
BmuI ACTGGG 1 cut(s) 228
BpiI GAAGAC 4 cut(s) 270, 1044, 1332, 1382
Bpu14I TTCGAA 1 cut(s) 290
BpuEI CTTGAG 5 cut(s) 224, 295, 1456, 1551, 1725
BpuMI CCSGG 1 cut(s) 302
BsaBI GATNNNNATC 2 cut(s) 114, 1260
BsaJI CCNNGG 1 cut(s) 156
BsaWI WCCGGW 3 cut(s) 803, 1365, 1614
BsaXI ACNNNNNCTCC 2 cut(s) 1853, 1883
Bsc4I CCNNNNNNNGG 3 cut(s) 205, 1204, 1340
Bse1I ACTGG 4 cut(s) 182, 223, 412, 1223
Bse8I GATNNNNATC 2 cut(s) 114, 1260
BseAI TCCGGA 2 cut(s) 1365, 1614
BseDI CCNNGG 1 cut(s) 156
BseGI GGATG 3 cut(s) 109, 727, 1543
BseJI GATNNNNATC 2 cut(s) 114, 1260
BseLI CCNNNNNNNGG 3 cut(s) 205, 1204, 1340
BseMII CTCAG 3 cut(s) 963, 1275, 1742
BseNI ACTGG 4 cut(s) 182, 223, 412, 1223
BseRI GAGGAG 2 cut(s) 1469, 1675
BseXI GCAGC 5 cut(s) 107, 706, 1234, 1258, 1708
BsgI GTGCAG 1 cut(s) 657
Bsh1285I CGRYCG 1 cut(s) 1121
BshFI GGCC 2 cut(s) 155, 1344
BshNI GGYRCC 1 cut(s) 1809
BsiEI CGRYCG 1 cut(s) 1121
BsiHKAI GWGCWC 3 cut(s) 174, 1308, 1773
BsiHKCI CYCGRG 2 cut(s) 1347, 1718
BsiSI CCGG 4 cut(s) 302, 804, 1366, 1615
BslFI GGGAC 1 cut(s) 399
BslI CCNNNNNNNGG 3 cut(s) 205, 1204, 1340
BsmAI GTCTC 4 cut(s) 635, 747, 1080, 1146
BsmFI GGGAC 1 cut(s) 399
BsnI GGCC 2 cut(s) 155, 1344
BsoBI CYCGRG 2 cut(s) 1347, 1718
Bsp119I TTCGAA 1 cut(s) 290
Bsp1286I GDGCHC 5 cut(s) 174, 564, 1308, 1565, 1773
Bsp13I TCCGGA 2 cut(s) 1365, 1614
Bsp1407I TGTACA 1 cut(s) 936
BspACI CCGC 1 cut(s) 1550
BspANI GGCC 2 cut(s) 155, 1344
BspCNI CTCAG 3 cut(s) 962, 1276, 1743
BspEI TCCGGA 2 cut(s) 1365, 1614
BspHI TCATGA 1 cut(s) 322
BspLI GGNNCC 3 cut(s) 1370, 1811, 1863
BspPI GGATC 2 cut(s) 1325, 1629
BspT104I TTCGAA 1 cut(s) 290
BspT107I GGYRCC 1 cut(s) 1809
BspTI CTTAAG 1 cut(s) 872
BsrGI TGTACA 1 cut(s) 936
BsrI ACTGG 4 cut(s) 182, 223, 412, 1223
BssECI CCNNGG 1 cut(s) 156
BssSI CACGAG 3 cut(s) 19, 173, 652
BssT1I CCWWGG 1 cut(s) 156
Bst2BI CACGAG 3 cut(s) 19, 173, 652
Bst4CI ACNGT 2 cut(s) 1126, 1296
Bst6I CTCTTC 1 cut(s) 1083
BstAFI CTTAAG 1 cut(s) 872
BstAUI TGTACA 1 cut(s) 936
BstBI TTCGAA 1 cut(s) 290
BstC8I GCNNGC 1 cut(s) 996
BstDEI CTNAG 3 cut(s) 949, 1284, 1751
BstF5I GGATG 3 cut(s) 109, 727, 1543
BstMAI GTCTC 4 cut(s) 635, 747, 1080, 1146
BstMCI CGRYCG 1 cut(s) 1121
BstNSI RCATGY 2 cut(s) 998, 1780
BstSCI CCNGG 1 cut(s) 300
BstSFI CTRYAG 2 cut(s) 276, 1010
BstV1I GCAGC 5 cut(s) 107, 706, 1234, 1258, 1708
BstV2I GAAGAC 4 cut(s) 270, 1044, 1332, 1382
BstX2I RGATCY 1 cut(s) 1621
BstYI RGATCY 1 cut(s) 1621
BsuI GTATCC 1 cut(s) 488
BsuRI GGCC 2 cut(s) 155, 1344
BtsCI GGATG 3 cut(s) 109, 727, 1543
BtsIMutI CAGTG 4 cut(s) 186, 878, 1216, 1281
Cac8I GCNNGC 1 cut(s) 996
CaiI CAGNNNCTG 2 cut(s) 188, 1093
CciI TCATGA 1 cut(s) 322
Cfr13I GGNCC 2 cut(s) 1343, 1552
Csp6I GTAC 3 cut(s) 216, 665, 937
CspCI CAANNNNNGTGG 2 cut(s) 837, 872
CviAII CATG 9 cut(s) 323, 755, 991, 995, 1439, 1444, 1777, 1814, 1884
CviQI GTAC 3 cut(s) 216, 665, 937
DdeI CTNAG 3 cut(s) 949, 1284, 1751
Eam1104I CTCTTC 1 cut(s) 1083
EarI CTCTTC 1 cut(s) 1083
Eco130I CCWWGG 1 cut(s) 156
Eco147I AGGCCT 1 cut(s) 155
Eco24I GRGCYC 2 cut(s) 564, 1565
Eco32I GATATC 1 cut(s) 1612
Eco47I GGWCC 1 cut(s) 1552
Eco57I CTGAAG 1 cut(s) 902
Eco88I CYCGRG 2 cut(s) 1347, 1718
EcoO109I RGGNCCY 1 cut(s) 1343
EcoRI GAATTC 2 cut(s) 38, 209
EcoRV GATATC 1 cut(s) 1612
EcoT14I CCWWGG 1 cut(s) 156
EcoT22I ATGCAT 1 cut(s) 996
EcoT38I GRGCYC 2 cut(s) 564, 1565
ErhI CCWWGG 1 cut(s) 156
FaeI CATG 9 cut(s) 326, 758, 994, 998, 1442, 1447, 1780, 1817, 1887
FaqI GGGAC 1 cut(s) 399
FatI CATG 9 cut(s) 322, 754, 990, 994, 1438, 1443, 1776, 1813, 1883
FbaI TGATCA 5 cut(s) 115, 190, 457, 592, 1255
FblI GTMKAC 1 cut(s) 845
Fnu4HI GCNGC 5 cut(s) 96, 695, 1223, 1247, 1697
FokI GGATG 3 cut(s) 116, 734, 1550
FriOI GRGCYC 2 cut(s) 564, 1565
Fsp4HI GCNGC 5 cut(s) 96, 695, 1223, 1247, 1697
FspBI CTAG 3 cut(s) 629, 662, 1823
GluI GCNGC 5 cut(s) 96, 695, 1223, 1247, 1697
HaeIII GGCC 2 cut(s) 155, 1344
HapII CCGG 4 cut(s) 302, 804, 1366, 1615
Hin1II CATG 9 cut(s) 326, 758, 994, 998, 1442, 1447, 1780, 1817, 1887
HincII GTYRAC 2 cut(s) 584, 1129
HindII GTYRAC 2 cut(s) 584, 1129
HindIII AAGCTT 1 cut(s) 869
HpaII CCGG 4 cut(s) 302, 804, 1366, 1615
HphI GGTGA 3 cut(s) 623, 1498, 1690
Hpy166II GTNNAC 8 cut(s) 335, 439, 584, 659, 846, 937, 1129, 1843
Hpy8I GTNNAC 8 cut(s) 335, 439, 584, 659, 846, 937, 1129, 1843
HpyAV CCTTC 6 cut(s) 925, 1391, 1420, 1627, 1842, 1875
HpyCH4III ACNGT 2 cut(s) 1126, 1296
HpyCH4IV ACGT 1 cut(s) 225
HpyCH4V TGCA 9 cut(s) 674, 694, 994, 998, 1159, 1246, 1312, 1422, 1510
HpyF3I CTNAG 3 cut(s) 949, 1284, 1751
HpySE526I ACGT 1 cut(s) 225
Hsp92II CATG 9 cut(s) 326, 758, 994, 998, 1442, 1447, 1780, 1817, 1887
Kpn2I TCCGGA 2 cut(s) 1365, 1614
Ksp22I TGATCA 5 cut(s) 115, 190, 457, 592, 1255
LmnI GCTCC 3 cut(s) 1311, 1597, 1861
Lsp1109I GCAGC 5 cut(s) 107, 706, 1234, 1258, 1708
LweI GCATC 5 cut(s) 100, 223, 1470, 1497, 1708
MaeI CTAG 3 cut(s) 629, 662, 1823
MaeII ACGT 1 cut(s) 225
MaeIII GTNAC 1 cut(s) 184
MfeI CAATTG 2 cut(s) 566, 999
MflI RGATCY 1 cut(s) 1621
MhlI GDGCHC 5 cut(s) 174, 564, 1308, 1565, 1773
MlyI GAGTC 6 cut(s) 444, 664, 749, 851, 1359, 1710
MmeI TCCRAC 1 cut(s) 1165
Mph1103I ATGCAT 1 cut(s) 996
MroI TCCGGA 2 cut(s) 1365, 1614
MroXI GAANNNNTTC 2 cut(s) 1212, 1685
MseI TTAA 7 cut(s) 90, 339, 777, 789, 873, 1059, 1888
MslI CAYNNNNRTG 1 cut(s) 1269
MspA1I CMGCKG 1 cut(s) 1015
MspCI CTTAAG 1 cut(s) 872
MspI CCGG 4 cut(s) 302, 804, 1366, 1615
MspR9I CCNGG 1 cut(s) 302
MunI CAATTG 2 cut(s) 566, 999
NciI CCSGG 1 cut(s) 302
NlaIII CATG 9 cut(s) 326, 758, 994, 998, 1442, 1447, 1780, 1817, 1887
NlaIV GGNNCC 3 cut(s) 1370, 1811, 1863
NmuCI GTSAC 1 cut(s) 184
NsiI ATGCAT 1 cut(s) 996
NspI RCATGY 2 cut(s) 998, 1780
NspV TTCGAA 1 cut(s) 290
PaeI GCATGC 1 cut(s) 998
PaeR7I CTCGAG 2 cut(s) 1347, 1718
PagI TCATGA 1 cut(s) 322
PceI AGGCCT 1 cut(s) 155
PciI ACATGT 1 cut(s) 1776
PdmI GAANNNNTTC 2 cut(s) 1212, 1685
PfeI GAWTC 8 cut(s) 134, 287, 408, 527, 862, 915, 925, 1317
PkrI GCNGC 5 cut(s) 97, 696, 1224, 1248, 1698
PleI GAGTC 6 cut(s) 443, 663, 748, 850, 1358, 1710
PpsI GAGTC 6 cut(s) 443, 663, 748, 850, 1358, 1710
PscI ACATGT 1 cut(s) 1776
PshBI ATTAAT 1 cut(s) 1059
Psp1406I AACGTT 1 cut(s) 225
PspN4I GGNNCC 3 cut(s) 1370, 1811, 1863
PspPI GGNCC 2 cut(s) 1343, 1552
PspXI VCTCGAGB 2 cut(s) 1347, 1718
PstNI CAGNNNCTG 2 cut(s) 188, 1093
PsuI RGATCY 1 cut(s) 1621
PvuII CAGCTG 1 cut(s) 1015
RsaI GTAC 3 cut(s) 217, 666, 938
RsaNI GTAC 3 cut(s) 216, 665, 937
RseI CAYNNNNRTG 1 cut(s) 1269
SaqAI TTAA 7 cut(s) 90, 339, 777, 789, 873, 1059, 1888
SatI GCNGC 5 cut(s) 96, 695, 1223, 1247, 1697
Sau96I GGNCC 2 cut(s) 1343, 1552
ScaI AGTACT 1 cut(s) 217
SchI GAGTC 6 cut(s) 444, 664, 749, 851, 1359, 1710
ScrFI CCNGG 1 cut(s) 302
SduI GDGCHC 5 cut(s) 174, 564, 1308, 1565, 1773
SfaNI GCATC 5 cut(s) 100, 223, 1470, 1497, 1708
SfcI CTRYAG 2 cut(s) 276, 1010
Sfr274I CTCGAG 2 cut(s) 1347, 1718
SfuI TTCGAA 1 cut(s) 290
SinI GGWCC 1 cut(s) 1552
SlaI CTCGAG 2 cut(s) 1347, 1718
SmiMI CAYNNNNRTG 1 cut(s) 1269
SmlI CTYRAG 8 cut(s) 203, 310, 872, 1347, 1471, 1566, 1718, 1740
SmoI CTYRAG 8 cut(s) 203, 310, 872, 1347, 1471, 1566, 1718, 1740
SpeI ACTAGT 2 cut(s) 628, 661
SphI GCATGC 1 cut(s) 998
SseBI AGGCCT 1 cut(s) 155
SsiI CCGC 1 cut(s) 1550
SspMI CTAG 3 cut(s) 629, 662, 1823
StuI AGGCCT 1 cut(s) 155
StyD4I CCNGG 1 cut(s) 300
StyI CCWWGG 1 cut(s) 156
TaaI ACNGT 2 cut(s) 1126, 1296
TaiI ACGT 1 cut(s) 228
TaqI TCGA 6 cut(s) 290, 516, 900, 913, 1348, 1719
TaqII GACCGA 1 cut(s) 1135
TatI WGTACW 3 cut(s) 215, 664, 936
TfiI GAWTC 8 cut(s) 134, 287, 408, 527, 862, 915, 925, 1317
Tru1I TTAA 7 cut(s) 90, 339, 777, 789, 873, 1059, 1888
Tru9I TTAA 7 cut(s) 90, 339, 777, 789, 873, 1059, 1888
TscAI CASTG 4 cut(s) 193, 885, 1223, 1288
TseFI GTSAC 1 cut(s) 184
TseI GCWGC 5 cut(s) 95, 694, 1222, 1246, 1696
Tsp45I GTSAC 1 cut(s) 184
TspDTI ATGAA 7 cut(s) 555, 741, 906, 1029, 1085, 1309, 1830
TspGWI ACGGA 1 cut(s) 501
TspRI CASTG 4 cut(s) 193, 885, 1223, 1288
Vha464I CTTAAG 1 cut(s) 872
VpaK11BI GGWCC 1 cut(s) 1552
VspI ATTAAT 1 cut(s) 1059
XapI RAATTY 7 cut(s) 38, 65, 81, 209, 1142, 1209, 1523
XceI RCATGY 2 cut(s) 998, 1780
XhoI CTCGAG 2 cut(s) 1347, 1718
XmiI GTMKAC 1 cut(s) 845
XmnI GAANNNNTTC 2 cut(s) 1212, 1685
XspI CTAG 3 cut(s) 629, 662, 1823
ZrmI AGTACT 1 cut(s) 217
Zsp2I ATGCAT 1 cut(s) 996
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.