MD16G1072900.v1.1

Protein of unknown function (DUF1666)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0014609
Physical Location & Seq
Reverse (-)
5130043 .. 5134510
4468 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1072900.v1.1.491

Sequence Viewer

Length: 2274 bp
ATGATGGTTACCAGGAAATTCCACGTGCTCAAAACCCAATTCGATTCCGTTGCTAAGGGTTTGCTGAGCAAGACCCTGTACGGAAACATGGTTGGAGTCGTTGGTTCTATGTGGGTTCTGGTTTGCAGCTACTTTTTATTTGTTTTTGGATTCGTAAGCAGATACATGCTCAGATTCCAGGAAGATAATCCGAGAGATCAAAGTCAGAGTTCTTGGATTTCAGACATGAATGTGTTCAAAGAAGAAGAAGAAGAAGAAGAAGACGCATTCTTTGATTCGTCTAATAGCAAAATTGATGAGGAACACAGAGAAGAAGAAACAGGGGATTTGGGTTTTTCGGAGACGGTGTCGGCCGCGAGCACTGATCCTTACGAGTTCAGGTATGGCAAAGATGCTAGTTGCTACATGGAAGAACCGAAAGCTATGAGCTTTGTTGTCCAAGAATTGTACGCAGACTGGAGCAATTCAAATACTTATGGTAATCAGGAGAAAACAGCGGCAGAAACAGAGGATGTCGATGATCAAGAAAAAACAGAGGATTTTGATGATGTTCGAGAAAAAGCGCATGACTTGGCTGAAAGTGTTTGTACAGAAGAGCACCCTTTTCAGAGTTTTAGTTTTATGAAGTATCCGGTTGCTGAAATTGCGCCTTCGGAGACGGTTAAGCATCCGGAAACTGCAAGTTTTGGTGAGCATGATTCATCAGATGATGTTCAATCGCTTGTCGAAAATCGTGCCGCGGTTTCTGATTCTAAACAAGAGTCGGTTACATCAAGAGGAGGAACTGATGAGGTGGATTCAATTACTTACGAGTTTGTGGTGGGAAGAAATGTGAGAGAGCGATTGGAACCTCAAAGCCTAGTGATCATGAAGGGAGATGAACAGACGGTTAAGATGAACACGGATGATTTTCAATTGCATGAATTTAGTATCCCAAATAGTAAAGAAGTTCTTTTTTCAGATTCAGAAAATGGTTCTGATGATGAATATATCGAATTATCAGACCCCCAAATTCAAGTTTCTGACTTTAAGGAGGAAGTTGGTCCTCAAATTGTAGAGCAATCAGCAGAAGAAATTGGATTGCAGGAGGCAAATTTCGGGTCGAATCGGGATGGCGAAGACGAGGATGAAGAGCCATGGGAGCATGATGATCTAATCGAGCAGCTGAAAAATGAAATAAGAAATGGGGGAGGCGGGAGGCTTCCGACAATTTTAGAGGAAGAAACTGAGGGTACAAAGACGGTGGATAATCTGAAGCTGAAGCCGCTGGTTATGGAGGAAAAGCTCGAATTCAAAGATCGAATGGCTGAGATTCAGAAGGTGTACAAAAGCTACGCAGAGAAGATGAAGAAGCTGGATATCTTGAACAACCAGACCATGCATGCAATTGGTTTTCTCCAGCTGAAGGACCAAGTGAGATCAATGTCAATCCAAAAATCTTCAACGTTTCCACTTGGGAAGTCTCTTATTTCACAAAACTTATGGGGATGCAAAACACAAAGGCACCCCACAGCTCTTGCTCCAATGCCAAAAGTGGTCGGGGACTTGCACAAAGACTTTGAATTGGTGTATGTAGGGCAGGTTTGCCTTTCTTGGGAAATACTTAACTGGCAGCATGGAAAAGCCCTAGAACTGCAGCAATATGACAATCAAACATCTCATCATCGATATAACCTAGTAGCTACTGAGTTCCAACTGTTTCAAGTGCTCCTTCAAAGATTCATAGAGGATGATCCGTTTCAAGGCCCCAGGGTCCAGCATTACGTGAGGAATCGATGCGTTCTTCGTAACCTTCTCCAAGTTCCCACCATCAAAGATGATAAGAAGCATCTAACAAGAGAGGAGGAGGAATCGATTATTTTGAGTGGAATGCTAGTGAAGATCATCGAAGAATCAATGCATATATTTTGGAACTTTGTTCGTACGGATGAGAGGAATGCCAACCTAAAGGGACTTCAAGAGACTCAGGTCGACCATAAGGATTTAGAGCTTCTCCTTGACATACGGAAAGATCTCCAAAAGAAGGAGAAAAAGCTTAAAGAAATACATAGAAGTGGAAATTGTGTAGTGAAGAAGTTCCAAAAGCACCAAGAGAGACGACTTGAGCACGATTTATTCACTGCTCAAGTTGAATTGAGGTTGATTTCAAGAGTGCTAAACTTGTCAAAAATCAAAACGGAGCAGTTGGTTTGGTGCCACCAAAAGCTGGAGAATATCAATTTTGTTCATAGGAAGGTTCATGTGGAGCCCTCGTTTTTGCTTTTCCCATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1666

Protein Analysis

758

Amino Acids

87.66

Weight (kDa)

4.89

Isoelectric Point (pI)

50.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1666 PF07891 518 - 756 1.2e-89 Protein of unknown function (DUF1666)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014609)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G69610 AT1G69610 AT1G69610
fragaria_vesca FvH4_4g31360
malus_domestica MD16G1072900.v1.1
prunus_persica Prupe.1G274600_v2.0.a1
pyrus_communis pycom16g06320
rosa_chinensis RchiOBHm_Chr4g0439871
rosa_laevigata RLG00000006231
rosa_multiflora Rmu_sc0000497.1_g000007
rosa_roxburghii Rroxscaffold_5G00380780
rosa_rugosa Rorug04G0321800
rosa_samantha Rh4AG372900 Rh4BG385300 Rh4CG399800 Rh4DG379500
rosa_wichuraiana Rw4G031880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1570
AccB1I GGYRCC 2 cut(s) 1503, 2193
AccB7I CCANNNNNTGG 1 cut(s) 2206
AccI GTMKAC 1 cut(s) 1971
AccII CGCG 2 cut(s) 356, 740
AccIII TCCGGA 1 cut(s) 670
AciI CCGC 6 cut(s) 354, 497, 738, 740, 1194, 1265
AclI AACGTT 1 cut(s) 1445
AclWI GGATC 2 cut(s) 359, 1727
AcoI YGGCCR 1 cut(s) 351
AcsI RAATTY 5 cut(s) 17, 923, 1011, 1093, 1289
AcuI CTGAAG 3 cut(s) 1274, 1280, 1424
AcvI CACGTG 1 cut(s) 25
AfaI GTAC 6 cut(s) 80, 449, 589, 1234, 1325, 1924
AfiI CCNNNNNNNGG 5 cut(s) 1405, 1594, 1742, 2023, 2206
AjnI CCWGG 3 cut(s) 11, 177, 1748
Alw21I GWGCWC 5 cut(s) 30, 362, 600, 1710, 2109
Alw26I GTCTC 5 cut(s) 335, 650, 1467, 1955, 2089
AlwI GGATC 2 cut(s) 359, 1727
Aor13HI TCCGGA 1 cut(s) 670
AoxI GGCC 2 cut(s) 351, 1744
ApeKI GCWGC 4 cut(s) 126, 1162, 1612, 1636
ApoI RAATTY 5 cut(s) 17, 923, 1011, 1093, 1289
ArsI GACNNNNNNTTYG 4 cut(s) 254, 286, 1085, 1117
Asp700I GAANNNNTTC 2 cut(s) 233, 2075
AspLEI GCGC 2 cut(s) 565, 649
AspS9I GGNCC 4 cut(s) 1043, 1408, 1745, 1753
AsuHPI GGTGA 1 cut(s) 701
AvaII GGWCC 3 cut(s) 1043, 1408, 1753
BanI GGYRCC 2 cut(s) 1503, 2193
BanII GRGCYC 1 cut(s) 2250
BbrPI CACGTG 1 cut(s) 25
BbsI GAAGAC 2 cut(s) 267, 1125
Bbv12I GWGCWC 5 cut(s) 30, 362, 600, 1710, 2109
BbvI GCAGC 4 cut(s) 138, 1174, 1624, 1648
BccI CCATC 2 cut(s) 1106, 1817
BcgI CGANNNNNNTGC 4 cut(s) 32, 66, 716, 750
BciT130I CCWGG 3 cut(s) 13, 179, 1750
BciVI GTATCC 2 cut(s) 639, 941
BclI TGATCA 2 cut(s) 520, 864
BcoDI GTCTC 5 cut(s) 335, 650, 1467, 1955, 2089
BfaI CTAG 5 cut(s) 396, 860, 1628, 1676, 1874
BfmI CTRYAG 1 cut(s) 1634
BfuAI ACCTGC 1 cut(s) 1570
BfuI GTATCC 2 cut(s) 639, 941
BglII AGATCT 1 cut(s) 2011
BisI GCNGC 8 cut(s) 127, 354, 498, 738, 1163, 1265, 1613, 1637
BlpI GCTNAGC 1 cut(s) 65
BlsI GCNGC 8 cut(s) 128, 355, 499, 739, 1164, 1266, 1614, 1638
Bme1390I CCNGG 3 cut(s) 13, 179, 1750
Bme18I GGWCC 3 cut(s) 1043, 1408, 1753
BmgT120I GGNCC 4 cut(s) 1043, 1408, 1745, 1753
BmiI GGNNCC 6 cut(s) 849, 1505, 1747, 1754, 2195, 2247
BmrFI CCNGG 3 cut(s) 13, 179, 1750
BmsI GCATC 5 cut(s) 382, 676, 1478, 1766, 1837
BoxI GACNNNNGTC 1 cut(s) 1967
BpiI GAAGAC 2 cut(s) 267, 1125
BpmI CTGGAG 3 cut(s) 478, 1382, 2228
Bpu10I CCTNAGC 1 cut(s) 54
Bpu1102I GCTNAGC 1 cut(s) 65
BpuEI CTTGAG 2 cut(s) 2109, 2123
Bsa29I ATCGAT 3 cut(s) 1666, 1774, 1853
BsaAI YACGTR 2 cut(s) 25, 1765
BsaJI CCNNGG 4 cut(s) 738, 1136, 1748, 1749
BsaWI WCCGGW 2 cut(s) 631, 670
BsaXI ACNNNNNCTCC 4 cut(s) 332, 362, 2237, 2267
Bsc4I CCNNNNNNNGG 5 cut(s) 1405, 1594, 1742, 2023, 2206
Bse1I ACTGG 2 cut(s) 461, 1613
BseAI TCCGGA 1 cut(s) 670
BseBI CCWGG 3 cut(s) 13, 179, 1750
BseCI ATCGAT 3 cut(s) 1666, 1774, 1853
BseDI CCNNGG 4 cut(s) 738, 1136, 1748, 1749
BseGI GGATG 8 cut(s) 517, 667, 910, 1117, 1132, 1493, 1735, 1933
BseLI CCNNNNNNNGG 5 cut(s) 1405, 1594, 1742, 2023, 2206
BseMII CTCAG 6 cut(s) 56, 184, 1218, 1299, 1677, 1979
BseNI ACTGG 2 cut(s) 461, 1613
BseRI GAGGAG 3 cut(s) 792, 1856, 1859
BseX3I CGGCCG 1 cut(s) 351
BseXI GCAGC 4 cut(s) 138, 1174, 1624, 1648
Bsh1236I CGCG 2 cut(s) 356, 740
Bsh1285I CGRYCG 1 cut(s) 354
BshFI GGCC 2 cut(s) 353, 1746
BshNI GGYRCC 2 cut(s) 1503, 2193
BshVI ATCGAT 3 cut(s) 1666, 1774, 1853
BsiEI CGRYCG 1 cut(s) 354
BsiHKAI GWGCWC 5 cut(s) 30, 362, 600, 1710, 2109
BsiSI CCGG 2 cut(s) 632, 671
BsiWI CGTACG 1 cut(s) 1922
BslFI GGGAC 2 cut(s) 1556, 1965
BslI CCNNNNNNNGG 5 cut(s) 1405, 1594, 1742, 2023, 2206
BsmAI GTCTC 5 cut(s) 335, 650, 1467, 1955, 2089
BsmBI CGTCTC 3 cut(s) 335, 650, 2089
BsmFI GGGAC 2 cut(s) 1556, 1965
BsmI GAATGC 3 cut(s) 266, 1875, 1942
BsnI GGCC 2 cut(s) 353, 1746
Bsp1286I GDGCHC 6 cut(s) 30, 362, 600, 1710, 2109, 2250
Bsp13I TCCGGA 1 cut(s) 670
Bsp1407I TGTACA 2 cut(s) 587, 1323
Bsp1720I GCTNAGC 1 cut(s) 65
Bsp19I CCATGG 1 cut(s) 1136
BspACI CCGC 6 cut(s) 354, 497, 738, 740, 1194, 1265
BspANI GGCC 2 cut(s) 353, 1746
BspCNI CTCAG 6 cut(s) 57, 183, 1219, 1300, 1678, 1978
BspDI ATCGAT 3 cut(s) 1666, 1774, 1853
BspEI TCCGGA 1 cut(s) 670
BspFNI CGCG 2 cut(s) 356, 740
BspHI TCATGA 1 cut(s) 867
BspLI GGNNCC 6 cut(s) 849, 1505, 1747, 1754, 2195, 2247
BspMAI CTGCAG 1 cut(s) 1638
BspMI ACCTGC 1 cut(s) 1570
BspPI GGATC 2 cut(s) 359, 1727
BspQI GCTCTTC 2 cut(s) 588, 1125
BspT107I GGYRCC 2 cut(s) 1503, 2193
BsrGI TGTACA 2 cut(s) 587, 1323
BsrI ACTGG 2 cut(s) 461, 1613
BssECI CCNNGG 4 cut(s) 738, 1136, 1748, 1749
BssT1I CCWWGG 1 cut(s) 1136
Bst2UI CCWGG 3 cut(s) 13, 179, 1750
Bst4CI ACNGT 5 cut(s) 346, 661, 889, 1243, 1698
Bst6I CTCTTC 2 cut(s) 588, 1125
BstAUI TGTACA 2 cut(s) 587, 1323
BstBAI YACGTR 2 cut(s) 25, 1765
BstC8I GCNNGC 2 cut(s) 358, 1383
BstDEI CTNAG 7 cut(s) 54, 65, 170, 1227, 1308, 1686, 1965
BstDSI CCRYGG 2 cut(s) 738, 1136
BstEII GGTNACC 1 cut(s) 7
BstF5I GGATG 8 cut(s) 517, 667, 910, 1117, 1132, 1493, 1735, 1933
BstFNI CGCG 2 cut(s) 356, 740
BstHHI GCGC 2 cut(s) 565, 649
BstMAI GTCTC 5 cut(s) 335, 650, 1467, 1955, 2089
BstMCI CGRYCG 1 cut(s) 354
BstMWI GCNNNNNNNGC 3 cut(s) 644, 1141, 1264
BstNI CCWGG 3 cut(s) 13, 179, 1750
BstNSI RCATGY 2 cut(s) 169, 1385
BstPAI GACNNNNGTC 1 cut(s) 1967
BstPI GGTNACC 1 cut(s) 7
BstSCI CCNGG 3 cut(s) 11, 177, 1748
BstSFI CTRYAG 1 cut(s) 1634
BstUI CGCG 2 cut(s) 356, 740
BstV1I GCAGC 4 cut(s) 138, 1174, 1624, 1648
BstV2I GAAGAC 2 cut(s) 267, 1125
BstX2I RGATCY 1 cut(s) 2011
BstYI RGATCY 1 cut(s) 2011
BstZI CGGCCG 1 cut(s) 351
Bsu15I ATCGAT 3 cut(s) 1666, 1774, 1853
BsuI GTATCC 2 cut(s) 639, 941
BsuRI GGCC 2 cut(s) 353, 1746
BsuTUI ATCGAT 3 cut(s) 1666, 1774, 1853
BtgI CCRYGG 2 cut(s) 738, 1136
BtsCI GGATG 8 cut(s) 517, 667, 910, 1117, 1132, 1493, 1735, 1933
BtsI GCAGTG 1 cut(s) 2118
BtsIMutI CAGTG 2 cut(s) 360, 2118
BveI ACCTGC 1 cut(s) 1570
Cac8I GCNNGC 2 cut(s) 358, 1383
CciI TCATGA 1 cut(s) 867
CfoI GCGC 2 cut(s) 565, 649
Cfr13I GGNCC 4 cut(s) 1043, 1408, 1745, 1753
Cfr42I CCGCGG 1 cut(s) 741
ClaI ATCGAT 3 cut(s) 1666, 1774, 1853
CseI GACGC 1 cut(s) 272
Csp6I GTAC 6 cut(s) 79, 448, 588, 1233, 1324, 1923
CspCI CAANNNNNGTGG 4 cut(s) 1224, 1259, 1498, 1533
CviQI GTAC 6 cut(s) 79, 448, 588, 1233, 1324, 1923
DdeI CTNAG 7 cut(s) 54, 65, 170, 1227, 1308, 1686, 1965
EaeI YGGCCR 1 cut(s) 351
EagI CGGCCG 1 cut(s) 351
Eam1104I CTCTTC 2 cut(s) 588, 1125
EarI CTCTTC 2 cut(s) 588, 1125
EclXI CGGCCG 1 cut(s) 351
Eco130I CCWWGG 1 cut(s) 1136
Eco24I GRGCYC 1 cut(s) 2250
Eco32I GATATC 1 cut(s) 1360
Eco47I GGWCC 3 cut(s) 1043, 1408, 1753
Eco52I CGGCCG 1 cut(s) 351
Eco57I CTGAAG 3 cut(s) 1274, 1280, 1424
Eco72I CACGTG 1 cut(s) 25
Eco91I GGTNACC 1 cut(s) 7
EcoO109I RGGNCCY 1 cut(s) 1745
EcoO65I GGTNACC 1 cut(s) 7
EcoRI GAATTC 1 cut(s) 1289
EcoRII CCWGG 3 cut(s) 11, 177, 1748
EcoRV GATATC 1 cut(s) 1360
EcoT14I CCWWGG 1 cut(s) 1136
EcoT22I ATGCAT 2 cut(s) 1383, 1902
EcoT38I GRGCYC 1 cut(s) 2250
ErhI CCWWGG 1 cut(s) 1136
Esp3I CGTCTC 3 cut(s) 335, 650, 2089
FalI AAGNNNNNCTT 4 cut(s) 936, 968, 1695, 1727
FaqI GGGAC 2 cut(s) 1556, 1965
FauI CCCGC 1 cut(s) 1187
FbaI TGATCA 2 cut(s) 520, 864
FblI GTMKAC 1 cut(s) 1971
Fnu4HI GCNGC 8 cut(s) 127, 354, 498, 738, 1163, 1265, 1613, 1637
FokI GGATG 8 cut(s) 524, 654, 917, 1124, 1139, 1500, 1742, 1940
FriOI GRGCYC 1 cut(s) 2250
Fsp4HI GCNGC 8 cut(s) 127, 354, 498, 738, 1163, 1265, 1613, 1637
FspBI CTAG 5 cut(s) 396, 860, 1628, 1676, 1874
GlaI GCGC 2 cut(s) 564, 648
GluI GCNGC 8 cut(s) 127, 354, 498, 738, 1163, 1265, 1613, 1637
GsuI CTGGAG 3 cut(s) 478, 1382, 2228
HaeIII GGCC 2 cut(s) 353, 1746
HapII CCGG 2 cut(s) 632, 671
HgaI GACGC 1 cut(s) 272
HhaI GCGC 2 cut(s) 565, 649
Hin6I GCGC 2 cut(s) 563, 647
HinP1I GCGC 2 cut(s) 563, 647
HincII GTYRAC 1 cut(s) 1972
HindII GTYRAC 1 cut(s) 1972
HindIII AAGCTT 1 cut(s) 2033
HpaII CCGG 2 cut(s) 632, 671
HphI GGTGA 1 cut(s) 701
Hpy166II GTNNAC 2 cut(s) 1324, 1972
Hpy8I GTNNAC 2 cut(s) 1324, 1972
HpyAV CCTTC 8 cut(s) 660, 865, 1312, 1399, 1721, 1802, 2017, 2227
HpyCH4III ACNGT 5 cut(s) 346, 661, 889, 1243, 1698
HpyCH4IV ACGT 3 cut(s) 24, 1445, 1764
HpyF10VI GCNNNNNNNGC 3 cut(s) 644, 1141, 1264
HpyF3I CTNAG 7 cut(s) 54, 65, 170, 1227, 1308, 1686, 1965
HpySE526I ACGT 3 cut(s) 24, 1445, 1764
HspAI GCGC 2 cut(s) 563, 647
Kpn2I TCCGGA 1 cut(s) 670
Ksp22I TGATCA 2 cut(s) 520, 864
KspI CCGCGG 1 cut(s) 741
LguI GCTCTTC 2 cut(s) 588, 1125
LmnI GCTCC 6 cut(s) 459, 1141, 1525, 1713, 2179, 2245
Lsp1109I GCAGC 4 cut(s) 138, 1174, 1624, 1648
LweI GCATC 5 cut(s) 382, 676, 1478, 1766, 1837
MaeI CTAG 5 cut(s) 396, 860, 1628, 1676, 1874
MaeII ACGT 3 cut(s) 24, 1445, 1764
MaeIII GTNAC 3 cut(s) 7, 766, 1787
MfeI CAATTG 2 cut(s) 914, 1386
MflI RGATCY 1 cut(s) 2011
MhlI GDGCHC 6 cut(s) 30, 362, 600, 1710, 2109, 2250
MlyI GAGTC 3 cut(s) 105, 770, 1957
MmeI TCCRAC 3 cut(s) 73, 1229, 1717
Mph1103I ATGCAT 2 cut(s) 1383, 1902
MroI TCCGGA 1 cut(s) 670
MroXI GAANNNNTTC 2 cut(s) 233, 2075
MseI TTAA 5 cut(s) 663, 891, 1029, 1605, 2037
MslI CAYNNNNRTG 2 cut(s) 230, 2052
MspA1I CMGCKG 5 cut(s) 497, 740, 1165, 1267, 1402
MspI CCGG 2 cut(s) 632, 671
MspR9I CCNGG 3 cut(s) 13, 179, 1750
MunI CAATTG 2 cut(s) 914, 1386
Mva1269I GAATGC 3 cut(s) 266, 1875, 1942
MvaI CCWGG 3 cut(s) 13, 179, 1750
MvnI CGCG 2 cut(s) 356, 740
MwoI GCNNNNNNNGC 3 cut(s) 644, 1141, 1264
NcoI CCATGG 1 cut(s) 1136
NlaIV GGNNCC 6 cut(s) 849, 1505, 1747, 1754, 2195, 2247
NsiI ATGCAT 2 cut(s) 1383, 1902
NspI RCATGY 2 cut(s) 169, 1385
PaeI GCATGC 1 cut(s) 1385
PagI TCATGA 1 cut(s) 867
PasI CCCWGGG 1 cut(s) 1749
PciSI GCTCTTC 2 cut(s) 588, 1125
PctI GAATGC 3 cut(s) 266, 1875, 1942
PdmI GAANNNNTTC 2 cut(s) 233, 2075
Pfl23II CGTACG 1 cut(s) 1922
PflFI GACNNNGTC 1 cut(s) 346
PflMI CCANNNNNTGG 1 cut(s) 2206
PfoI TCCNGGA 1 cut(s) 177
PkrI GCNGC 8 cut(s) 128, 355, 499, 739, 1164, 1266, 1614, 1638
PleI GAGTC 3 cut(s) 104, 769, 1957
PmaCI CACGTG 1 cut(s) 25
PmlI CACGTG 1 cut(s) 25
PpsI GAGTC 3 cut(s) 104, 769, 1957
Ppu21I YACGTR 2 cut(s) 25, 1765
PshAI GACNNNNGTC 1 cut(s) 1967
Psp1406I AACGTT 1 cut(s) 1445
Psp6I CCWGG 3 cut(s) 11, 177, 1748
PspCI CACGTG 1 cut(s) 25
PspEI GGTNACC 1 cut(s) 7
PspGI CCWGG 3 cut(s) 11, 177, 1748
PspLI CGTACG 1 cut(s) 1922
PspN4I GGNNCC 6 cut(s) 849, 1505, 1747, 1754, 2195, 2247
PspPI GGNCC 4 cut(s) 1043, 1408, 1745, 1753
PstI CTGCAG 1 cut(s) 1638
PsuI RGATCY 1 cut(s) 2011
PsyI GACNNNGTC 1 cut(s) 346
PvuII CAGCTG 2 cut(s) 1165, 1402
RsaI GTAC 6 cut(s) 80, 449, 589, 1234, 1325, 1924
RsaNI GTAC 6 cut(s) 79, 448, 588, 1233, 1324, 1923
RseI CAYNNNNRTG 2 cut(s) 230, 2052
SacII CCGCGG 1 cut(s) 741
SalI GTCGAC 1 cut(s) 1970
SapI GCTCTTC 2 cut(s) 588, 1125
SaqAI TTAA 5 cut(s) 663, 891, 1029, 1605, 2037
SatI GCNGC 8 cut(s) 127, 354, 498, 738, 1163, 1265, 1613, 1637
Sau96I GGNCC 4 cut(s) 1043, 1408, 1745, 1753
SchI GAGTC 3 cut(s) 105, 770, 1957
ScrFI CCNGG 3 cut(s) 13, 179, 1750
SduI GDGCHC 6 cut(s) 30, 362, 600, 1710, 2109, 2250
SfaNI GCATC 5 cut(s) 382, 676, 1478, 1766, 1837
SfcI CTRYAG 1 cut(s) 1634
Sfr303I CCGCGG 1 cut(s) 741
SgrBI CCGCGG 1 cut(s) 741
SinI GGWCC 3 cut(s) 1043, 1408, 1753
SmiMI CAYNNNNRTG 2 cut(s) 230, 2052
SmlI CTYRAG 2 cut(s) 2102, 2124
SmoI CTYRAG 2 cut(s) 2102, 2124
SphI GCATGC 1 cut(s) 1385
SsiI CCGC 6 cut(s) 354, 497, 738, 740, 1194, 1265
SspMI CTAG 5 cut(s) 396, 860, 1628, 1676, 1874
StyD4I CCNGG 3 cut(s) 11, 177, 1748
StyI CCWWGG 1 cut(s) 1136
TaaI ACNGT 5 cut(s) 346, 661, 889, 1243, 1698
TaiI ACGT 3 cut(s) 27, 1448, 1767
TatI WGTACW 2 cut(s) 587, 1323
TauI GCSGC 4 cut(s) 356, 500, 740, 1267
Tru1I TTAA 5 cut(s) 663, 891, 1029, 1605, 2037
Tru9I TTAA 5 cut(s) 663, 891, 1029, 1605, 2037
TscAI CASTG 2 cut(s) 367, 2125
TseI GCWGC 4 cut(s) 126, 1162, 1612, 1636
TspGWI ACGGA 7 cut(s) 37, 96, 917, 1725, 1940, 2020, 2192
TspRI CASTG 2 cut(s) 367, 2125
Tth111I GACNNNGTC 1 cut(s) 346
Van91I CCANNNNNTGG 1 cut(s) 2206
VpaK11BI GGWCC 3 cut(s) 1043, 1408, 1753
XapI RAATTY 5 cut(s) 17, 923, 1011, 1093, 1289
XceI RCATGY 2 cut(s) 169, 1385
XmiI GTMKAC 1 cut(s) 1971
XmnI GAANNNNTTC 2 cut(s) 233, 2075
XspI CTAG 5 cut(s) 396, 860, 1628, 1676, 1874
Zsp2I ATGCAT 2 cut(s) 1383, 1902
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.