Rh4AG372900

Protein of unknown function (DUF1666)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0014609
Physical Location & Seq
Reverse (-)
68082422 .. 68086719
4298 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG372900.1

Sequence Viewer

Length: 2187 bp
ATGAAGAATTCCGATCAAAACAGAGCAGAAACAGAGGGTTCCGTTCAAAACAGAGCAGAAAGAGAGGATTTCGATCAAAACAGAGCAGAAACGGATAATTCAGATCAAAACAGAGCAGAAACAGAGGATTCCGGTCAAAACAGAGCAGAAACGGAGAATTCCTATCAAAACAGAGCAGAAACGGAGAATTCCGATCAAAACAGAGCAGAAACAGAGAATTCCGGTTTTGTGGAGACCGTCTCTGCTGTAACCACCGATTTGTATGAGTATACTTCTAATAGAGTTATCAATGGCTATATGGAAGAACCAAAAGCCACGAGCTTCATTGTCCAAGAATTCTATGTTGATTGGGGCAGTTCTAAATTCTCTGATTATCAAATTCCTATTAAAGCAGCAGAGGATGATGATGCTGATCAGGAAAAAGTAGTGGATTCTATTGAAAGCGTTGAGGCCTTGGAGAATGGAGAGCACGAGAAACCAGTCACTGATCAAAACCCTCTTGAGGAATTCAGTACTGGGAACGTTTTAGATGCTGAAATTGAGCTTTGTGAGGTTGCAGAAGACAATTTCTCTGTAGTTTCTGATTCGAAACAAGACCCGGTTAGCTCAAGAAAAGATCATGAAGTGGGTTCACTTAACTACGAGTTTTTGATATACAGAAATGTGGTTCAGTTTGATGAGGGACTTGACCCTCAAAGCTCTGGATTCCAGTATGATGAGGAAGATCATAGAGTCCACTTTGATATGGAAGATGATCAGTTGCTTGATGTAAGTATCCCAGACGGAAAAGCAGTTGTCTTTTCCGATCAACTCGATGGTTCTGAATCTCCTGATGATGAATACATTGAATTGTTAGAGCCCCAATTGAGAAGTTATAGTCAACGACTTGATCAAGTTGTGGAGAGAGGTGAAGAAAAAGAGGAACTAGTCCAAGTTGAGACAGAAGCCACGAGTCCACTAGTACAATCTGCACAAGAAGAAATAGGATTGCAGCCTAAAAAGTTTGGATTGTATTCGGATGATGAAAACGAATTGGAGTCTCCTGACGAGCATGATGATCTAATTGAACAGATTAAAAGAGAAATTAAGAATGTGAGAACCGGAGGGCTTCCCACTATTTCAGAAGAAGATCATTCGGAGTCTACAAGAATGGTGGAGAATCTAAAGCTTAAGCCACTGAAGATTGATGAAAAGCTCGAATACAAAGATCGAATCGCAGAGATTCAGAAGGTGTACAAAAGCTACGCAGAGAAAATGCGAAAGCTGGACATTTTGAACAACCAGACCATGCATGCAATTGGTTTTCTACAGCTGAAAGACCAAGATAAGCCAATTTCATTGGAAAAGTCTTCCATTAATCCAATTATGAAGTCTCTTCTTTCGCAGAACCTGTTGAGGTGTAAAGCACAAAGACGACCGACAGTTGACCCTATGCCGAAATTTGTGGGAGACTTGCACAAGGACTTGGAATTGGTCTATGTTGGACAGGTTTGCCTCTCTTGGGAAATTCTTCACTGGCAGCACAGAAAAGCCCGAGAGTTGCAGCAATATGATCATCATCACTATAATGTTGTTGCCACTGAGTTTCAACTGTTTCAAGTGCTCCTGCAAAGATTCATAGAAGACGATCCATTTCAAGGCCCTCGAGTCCGGCACTATGTCCGGAACCGATGTGTCCTTCGTAGTCTTCTTCAAGTTCCAGCCATTAGAGATGATTGCAGGAAGGATAAGAAGCATGACATGGGAGGGGAGGAGGATTTGATAATCTTAAGTGAGATGCTGGTGAAAATCATTGAAGAAACGATGCAGATATTTTGGAAATTTCTTCGCAAGGATGAGAGAAATGCGGTCCTAAAGAGCCTTCAAGAAAGTCAGGTTGAGCATAAGGACTTGGAGCTTCTTGTAGATATCCGGAGGGATCTTCAAAAGAAGGAGAAAAAACTCAAAGACATACAGAGGAGTGGAAATTGTGTAGTGAAGAAGTTCCAAAAGCAGCATCAAGATCAAGACAGACTCGAGCATAGTTTTTTTGTCGCTCAAGTTGAACTGAGGTTGGTTTCAAGAGTGCTCAACATGTCAAAACTAACATCAGATCAGTTAGTTTGGTGCCATGAAAAGCTAGACAACATCAATTTTGTTCACAGAAGGGTTCTTTTGGAGCCTTCGTTTCTTCTTTTTCCATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1666

Protein Analysis

728

Amino Acids

84.76

Weight (kDa)

4.75

Isoelectric Point (pI)

47.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1666 PF07891 487 - 727 1.4e-88 Protein of unknown function (DUF1666)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014609)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G69610 AT1G69610 AT1G69610
fragaria_vesca FvH4_4g31360
malus_domestica MD16G1072900.v1.1
prunus_persica Prupe.1G274600_v2.0.a1
pyrus_communis pycom16g06320
rosa_chinensis RchiOBHm_Chr4g0439871
rosa_laevigata RLG00000006231
rosa_multiflora Rmu_sc0000497.1_g000007
rosa_roxburghii Rroxscaffold_5G00380780
rosa_rugosa Rorug04G0321800
rosa_samantha Rh4AG372900 Rh4BG385300 Rh4CG399800 Rh4DG379500
rosa_wichuraiana Rw4G031880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 2106
AccI GTMKAC 2 cut(s) 269, 1142
AccIII TCCGGA 2 cut(s) 1662, 1911
AciI CCGC 1 cut(s) 1847
AclI AACGTT 1 cut(s) 522
AclWI GGATC 2 cut(s) 1622, 1926
AcuI CTGAAG 1 cut(s) 1199
AfaI GTAC 3 cut(s) 514, 963, 1235
AfiI CCNNNNNNNGG 3 cut(s) 502, 1501, 1637
AflII CTTAAG 2 cut(s) 1169, 1768
AflIII ACRYGT 1 cut(s) 2073
AhlI ACTAGT 2 cut(s) 925, 958
Alw21I GWGCWC 3 cut(s) 471, 1605, 2070
Alw26I GTCTC 6 cut(s) 227, 244, 932, 1044, 1377, 1443
AlwI GGATC 2 cut(s) 1622, 1926
AlwNI CAGNNNCTG 2 cut(s) 485, 1390
Ama87I CYCGRG 3 cut(s) 1533, 1644, 2015
Aor13HI TCCGGA 2 cut(s) 1662, 1911
AoxI GGCC 2 cut(s) 450, 1639
ApeKI GCWGC 5 cut(s) 392, 991, 1519, 1543, 1993
AseI ATTAAT 1 cut(s) 1356
Asp700I GAANNNNTTC 2 cut(s) 1509, 1982
AspS9I GGNCC 2 cut(s) 1640, 1849
AsuC2I CCSGG 1 cut(s) 599
AsuHPI GGTGA 2 cut(s) 920, 1795
AsuII TTCGAA 1 cut(s) 587
AvaI CYCGRG 3 cut(s) 1533, 1644, 2015
AvaII GGWCC 1 cut(s) 1849
BanI GGYRCC 1 cut(s) 2106
BanII GRGCYC 1 cut(s) 861
BauI CACGAG 3 cut(s) 316, 470, 949
BbsI GAAGAC 4 cut(s) 567, 1341, 1629, 1679
Bbv12I GWGCWC 3 cut(s) 471, 1605, 2070
BbvI GCAGC 5 cut(s) 404, 1003, 1531, 1555, 2005
BccI CCATC 1 cut(s) 809
BciVI GTATCC 1 cut(s) 785
BclI TGATCA 5 cut(s) 412, 487, 754, 889, 1552
BcnI CCSGG 1 cut(s) 599
BcoDI GTCTC 6 cut(s) 227, 244, 932, 1044, 1377, 1443
BcuI ACTAGT 2 cut(s) 925, 958
BfaI CTAG 3 cut(s) 926, 959, 2120
BfmI CTRYAG 2 cut(s) 573, 1307
BfrI CTTAAG 2 cut(s) 1169, 1768
BfuI GTATCC 1 cut(s) 785
BisI GCNGC 5 cut(s) 393, 992, 1520, 1544, 1994
BlsI GCNGC 5 cut(s) 394, 993, 1521, 1545, 1995
BmcAI AGTACT 1 cut(s) 514
Bme1390I CCNGG 1 cut(s) 599
Bme18I GGWCC 1 cut(s) 1849
BmeT110I CYCGRG 3 cut(s) 1533, 1644, 2015
BmgT120I GGNCC 2 cut(s) 1640, 1849
BmiI GGNNCC 4 cut(s) 40, 1667, 2108, 2160
BmrFI CCNGG 1 cut(s) 599
BmrI ACTGGG 1 cut(s) 525
BmsI GCATC 5 cut(s) 397, 520, 1767, 1794, 2005
BmuI ACTGGG 1 cut(s) 525
BpiI GAAGAC 4 cut(s) 567, 1341, 1629, 1679
BplI GAGNNNNNCTC 2 cut(s) 224, 256
Bpu14I TTCGAA 1 cut(s) 587
BpuEI CTTGAG 3 cut(s) 521, 592, 2022
BpuMI CCSGG 1 cut(s) 599
BsaBI GATNNNNATC 3 cut(s) 72, 411, 1557
BsaI GGTCTC 1 cut(s) 227
BsaJI CCNNGG 1 cut(s) 453
BsaWI WCCGGW 5 cut(s) 131, 221, 1100, 1662, 1911
BsaXI ACNNNNNCTCC 2 cut(s) 2150, 2180
Bsc4I CCNNNNNNNGG 3 cut(s) 502, 1501, 1637
Bse1I ACTGG 4 cut(s) 479, 520, 709, 1520
Bse8I GATNNNNATC 3 cut(s) 72, 411, 1557
BseAI TCCGGA 2 cut(s) 1662, 1911
BseDI CCNNGG 1 cut(s) 453
BseGI GGATG 3 cut(s) 406, 1024, 1840
BseJI GATNNNNATC 3 cut(s) 72, 411, 1557
BseLI CCNNNNNNNGG 3 cut(s) 502, 1501, 1637
BseMII CTCAG 2 cut(s) 1572, 2039
BseNI ACTGG 4 cut(s) 479, 520, 709, 1520
BseRI GAGGAG 2 cut(s) 1766, 1972
BseXI GCAGC 5 cut(s) 404, 1003, 1531, 1555, 2005
BsgI GTGCAG 1 cut(s) 954
Bsh1285I CGRYCG 1 cut(s) 1418
BshFI GGCC 2 cut(s) 452, 1641
BshNI GGYRCC 1 cut(s) 2106
BsiEI CGRYCG 1 cut(s) 1418
BsiHKAI GWGCWC 3 cut(s) 471, 1605, 2070
BsiHKCI CYCGRG 3 cut(s) 1533, 1644, 2015
BsiSI CCGG 7 cut(s) 132, 222, 599, 1101, 1651, 1663, 1912
BslFI GGGAC 1 cut(s) 696
BslI CCNNNNNNNGG 3 cut(s) 502, 1501, 1637
BsmAI GTCTC 6 cut(s) 227, 244, 932, 1044, 1377, 1443
BsmBI CGTCTC 1 cut(s) 244
BsmFI GGGAC 1 cut(s) 696
BsnI GGCC 2 cut(s) 452, 1641
Bso31I GGTCTC 1 cut(s) 227
BsoBI CYCGRG 3 cut(s) 1533, 1644, 2015
Bsp119I TTCGAA 1 cut(s) 587
Bsp1286I GDGCHC 4 cut(s) 471, 861, 1605, 2070
Bsp13I TCCGGA 2 cut(s) 1662, 1911
Bsp1407I TGTACA 1 cut(s) 1233
BspACI CCGC 1 cut(s) 1847
BspANI GGCC 2 cut(s) 452, 1641
BspCNI CTCAG 2 cut(s) 1573, 2040
BspEI TCCGGA 2 cut(s) 1662, 1911
BspHI TCATGA 1 cut(s) 619
BspLI GGNNCC 4 cut(s) 40, 1667, 2108, 2160
BspPI GGATC 2 cut(s) 1622, 1926
BspT104I TTCGAA 1 cut(s) 587
BspT107I GGYRCC 1 cut(s) 2106
BspTI CTTAAG 2 cut(s) 1169, 1768
BspTNI GGTCTC 1 cut(s) 227
BsrGI TGTACA 1 cut(s) 1233
BsrI ACTGG 4 cut(s) 479, 520, 709, 1520
BssECI CCNNGG 1 cut(s) 453
BssNAI GTATAC 1 cut(s) 270
BssSI CACGAG 3 cut(s) 316, 470, 949
BssT1I CCWWGG 1 cut(s) 453
Bst1107I GTATAC 1 cut(s) 270
Bst2BI CACGAG 3 cut(s) 316, 470, 949
Bst4CI ACNGT 3 cut(s) 238, 1423, 1593
Bst6I CTCTTC 1 cut(s) 1380
BstAFI CTTAAG 2 cut(s) 1169, 1768
BstAUI TGTACA 1 cut(s) 1233
BstBI TTCGAA 1 cut(s) 587
BstC8I GCNNGC 1 cut(s) 1293
BstDEI CTNAG 2 cut(s) 1581, 2048
BstF5I GGATG 3 cut(s) 406, 1024, 1840
BstMAI GTCTC 6 cut(s) 227, 244, 932, 1044, 1377, 1443
BstMCI CGRYCG 1 cut(s) 1418
BstNSI RCATGY 2 cut(s) 1295, 2077
BstSCI CCNGG 1 cut(s) 597
BstSFI CTRYAG 2 cut(s) 573, 1307
BstV1I GCAGC 5 cut(s) 404, 1003, 1531, 1555, 2005
BstV2I GAAGAC 4 cut(s) 567, 1341, 1629, 1679
BstX2I RGATCY 1 cut(s) 1918
BstYI RGATCY 1 cut(s) 1918
BstZ17I GTATAC 1 cut(s) 270
BsuI GTATCC 1 cut(s) 785
BsuRI GGCC 2 cut(s) 452, 1641
BtsCI GGATG 3 cut(s) 406, 1024, 1840
BtsIMutI CAGTG 4 cut(s) 483, 1175, 1513, 1578
Cac8I GCNNGC 1 cut(s) 1293
CaiI CAGNNNCTG 2 cut(s) 485, 1390
CciI TCATGA 1 cut(s) 619
Cfr13I GGNCC 2 cut(s) 1640, 1849
Csp6I GTAC 3 cut(s) 513, 962, 1234
CspCI CAANNNNNGTGG 2 cut(s) 1134, 1169
CviAII CATG 9 cut(s) 620, 1052, 1288, 1292, 1736, 1741, 2074, 2111, 2181
CviQI GTAC 3 cut(s) 513, 962, 1234
DdeI CTNAG 2 cut(s) 1581, 2048
Eam1104I CTCTTC 1 cut(s) 1380
EarI CTCTTC 1 cut(s) 1380
Eco130I CCWWGG 1 cut(s) 453
Eco147I AGGCCT 1 cut(s) 452
Eco24I GRGCYC 1 cut(s) 861
Eco31I GGTCTC 1 cut(s) 227
Eco32I GATATC 1 cut(s) 1909
Eco47I GGWCC 1 cut(s) 1849
Eco57I CTGAAG 1 cut(s) 1199
Eco88I CYCGRG 3 cut(s) 1533, 1644, 2015
EcoO109I RGGNCCY 1 cut(s) 1640
EcoRI GAATTC 6 cut(s) 7, 157, 187, 217, 335, 506
EcoRV GATATC 1 cut(s) 1909
EcoT14I CCWWGG 1 cut(s) 453
EcoT22I ATGCAT 1 cut(s) 1293
EcoT38I GRGCYC 1 cut(s) 861
ErhI CCWWGG 1 cut(s) 453
Esp3I CGTCTC 1 cut(s) 244
FaeI CATG 9 cut(s) 623, 1055, 1291, 1295, 1739, 1744, 2077, 2114, 2184
FaqI GGGAC 1 cut(s) 696
FatI CATG 9 cut(s) 619, 1051, 1287, 1291, 1735, 1740, 2073, 2110, 2180
FbaI TGATCA 5 cut(s) 412, 487, 754, 889, 1552
FblI GTMKAC 2 cut(s) 269, 1142
Fnu4HI GCNGC 5 cut(s) 393, 992, 1520, 1544, 1994
FokI GGATG 3 cut(s) 413, 1031, 1847
FriOI GRGCYC 1 cut(s) 861
Fsp4HI GCNGC 5 cut(s) 393, 992, 1520, 1544, 1994
FspBI CTAG 3 cut(s) 926, 959, 2120
GluI GCNGC 5 cut(s) 393, 992, 1520, 1544, 1994
HaeIII GGCC 2 cut(s) 452, 1641
HapII CCGG 7 cut(s) 132, 222, 599, 1101, 1651, 1663, 1912
Hin1II CATG 9 cut(s) 623, 1055, 1291, 1295, 1739, 1744, 2077, 2114, 2184
HincII GTYRAC 2 cut(s) 881, 1426
HindII GTYRAC 2 cut(s) 881, 1426
HindIII AAGCTT 1 cut(s) 1166
HpaII CCGG 7 cut(s) 132, 222, 599, 1101, 1651, 1663, 1912
HphI GGTGA 2 cut(s) 920, 1795
Hpy166II GTNNAC 9 cut(s) 270, 632, 736, 881, 956, 1143, 1234, 1426, 2140
Hpy8I GTNNAC 9 cut(s) 270, 632, 736, 881, 956, 1143, 1234, 1426, 2140
HpyAV CCTTC 7 cut(s) 1222, 1688, 1717, 1871, 1924, 2139, 2172
HpyCH4III ACNGT 3 cut(s) 238, 1423, 1593
HpyCH4IV ACGT 1 cut(s) 522
HpyF3I CTNAG 2 cut(s) 1581, 2048
HpySE526I ACGT 1 cut(s) 522
Hsp92II CATG 9 cut(s) 623, 1055, 1291, 1295, 1739, 1744, 2077, 2114, 2184
Kpn2I TCCGGA 2 cut(s) 1662, 1911
Ksp22I TGATCA 5 cut(s) 412, 487, 754, 889, 1552
LmnI GCTCC 3 cut(s) 1608, 1894, 2158
Lsp1109I GCAGC 5 cut(s) 404, 1003, 1531, 1555, 2005
LweI GCATC 5 cut(s) 397, 520, 1767, 1794, 2005
MaeI CTAG 3 cut(s) 926, 959, 2120
MaeII ACGT 1 cut(s) 522
MaeIII GTNAC 2 cut(s) 247, 481
MfeI CAATTG 2 cut(s) 863, 1296
MflI RGATCY 1 cut(s) 1918
MhlI GDGCHC 4 cut(s) 471, 861, 1605, 2070
MlyI GAGTC 6 cut(s) 741, 961, 1046, 1148, 1656, 2007
MmeI TCCRAC 1 cut(s) 1462
Mph1103I ATGCAT 1 cut(s) 1293
MroI TCCGGA 2 cut(s) 1662, 1911
MroXI GAANNNNTTC 2 cut(s) 1509, 1982
MseI TTAA 8 cut(s) 387, 636, 1074, 1086, 1170, 1356, 1769, 2185
MslI CAYNNNNRTG 1 cut(s) 1566
MspA1I CMGCKG 1 cut(s) 1312
MspCI CTTAAG 2 cut(s) 1169, 1768
MspI CCGG 7 cut(s) 132, 222, 599, 1101, 1651, 1663, 1912
MspR9I CCNGG 1 cut(s) 599
MunI CAATTG 2 cut(s) 863, 1296
NciI CCSGG 1 cut(s) 599
NlaIII CATG 9 cut(s) 623, 1055, 1291, 1295, 1739, 1744, 2077, 2114, 2184
NlaIV GGNNCC 4 cut(s) 40, 1667, 2108, 2160
NmuCI GTSAC 1 cut(s) 481
NsiI ATGCAT 1 cut(s) 1293
NspI RCATGY 2 cut(s) 1295, 2077
NspV TTCGAA 1 cut(s) 587
PaeI GCATGC 1 cut(s) 1295
PaeR7I CTCGAG 2 cut(s) 1644, 2015
PagI TCATGA 1 cut(s) 619
PceI AGGCCT 1 cut(s) 452
PciI ACATGT 1 cut(s) 2073
PdmI GAANNNNTTC 2 cut(s) 1509, 1982
PfeI GAWTC 9 cut(s) 128, 431, 584, 705, 824, 1159, 1212, 1222, 1614
PkrI GCNGC 5 cut(s) 394, 993, 1521, 1545, 1995
PleI GAGTC 6 cut(s) 740, 960, 1045, 1147, 1655, 2007
PpsI GAGTC 6 cut(s) 740, 960, 1045, 1147, 1655, 2007
PscI ACATGT 1 cut(s) 2073
PshBI ATTAAT 1 cut(s) 1356
Psp1406I AACGTT 1 cut(s) 522
PspN4I GGNNCC 4 cut(s) 40, 1667, 2108, 2160
PspPI GGNCC 2 cut(s) 1640, 1849
PspXI VCTCGAGB 2 cut(s) 1644, 2015
PstNI CAGNNNCTG 2 cut(s) 485, 1390
PsuI RGATCY 1 cut(s) 1918
PvuII CAGCTG 1 cut(s) 1312
RsaI GTAC 3 cut(s) 514, 963, 1235
RsaNI GTAC 3 cut(s) 513, 962, 1234
RseI CAYNNNNRTG 1 cut(s) 1566
SaqAI TTAA 8 cut(s) 387, 636, 1074, 1086, 1170, 1356, 1769, 2185
SatI GCNGC 5 cut(s) 393, 992, 1520, 1544, 1994
Sau96I GGNCC 2 cut(s) 1640, 1849
ScaI AGTACT 1 cut(s) 514
SchI GAGTC 6 cut(s) 741, 961, 1046, 1148, 1656, 2007
ScrFI CCNGG 1 cut(s) 599
SduI GDGCHC 4 cut(s) 471, 861, 1605, 2070
SfaNI GCATC 5 cut(s) 397, 520, 1767, 1794, 2005
SfcI CTRYAG 2 cut(s) 573, 1307
Sfr274I CTCGAG 2 cut(s) 1644, 2015
SfuI TTCGAA 1 cut(s) 587
SinI GGWCC 1 cut(s) 1849
SlaI CTCGAG 2 cut(s) 1644, 2015
SmiMI CAYNNNNRTG 1 cut(s) 1566
SmlI CTYRAG 7 cut(s) 500, 607, 1169, 1644, 1768, 2015, 2037
SmoI CTYRAG 7 cut(s) 500, 607, 1169, 1644, 1768, 2015, 2037
SpeI ACTAGT 2 cut(s) 925, 958
SphI GCATGC 1 cut(s) 1295
SseBI AGGCCT 1 cut(s) 452
SsiI CCGC 1 cut(s) 1847
SspMI CTAG 3 cut(s) 926, 959, 2120
StuI AGGCCT 1 cut(s) 452
StyD4I CCNGG 1 cut(s) 597
StyI CCWWGG 1 cut(s) 453
TaaI ACNGT 3 cut(s) 238, 1423, 1593
TaiI ACGT 1 cut(s) 525
TaqI TCGA 7 cut(s) 72, 587, 813, 1197, 1210, 1645, 2016
TaqII GACCGA 1 cut(s) 1432
TatI WGTACW 3 cut(s) 512, 961, 1233
TfiI GAWTC 9 cut(s) 128, 431, 584, 705, 824, 1159, 1212, 1222, 1614
Tru1I TTAA 8 cut(s) 387, 636, 1074, 1086, 1170, 1356, 1769, 2185
Tru9I TTAA 8 cut(s) 387, 636, 1074, 1086, 1170, 1356, 1769, 2185
TscAI CASTG 4 cut(s) 490, 1182, 1520, 1585
TseFI GTSAC 1 cut(s) 481
TseI GCWGC 5 cut(s) 392, 991, 1519, 1543, 1993
Tsp45I GTSAC 1 cut(s) 481
TspGWI ACGGA 5 cut(s) 31, 107, 167, 197, 798
TspRI CASTG 4 cut(s) 490, 1182, 1520, 1585
Vha464I CTTAAG 2 cut(s) 1169, 1768
VpaK11BI GGWCC 1 cut(s) 1849
VspI ATTAAT 1 cut(s) 1356
XceI RCATGY 2 cut(s) 1295, 2077
XhoI CTCGAG 2 cut(s) 1644, 2015
XmiI GTMKAC 2 cut(s) 269, 1142
XmnI GAANNNNTTC 2 cut(s) 1509, 1982
XspI CTAG 3 cut(s) 926, 959, 2120
ZrmI AGTACT 1 cut(s) 514
Zsp2I ATGCAT 1 cut(s) 1293
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.