Rh4CG399800

Protein of unknown function (DUF1666)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0014609
Physical Location & Seq
Reverse (-)
64860658 .. 64864502
3845 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG399800.1

Sequence Viewer

Length: 2400 bp
ATGAACGTTTTTTATTATTATTATTATCATTCTCTCAGATTTCAGGCAGATGATCTCAGAAATCATAACCCTGTTGTTTGTTTGGAGCGTGATCATCAATTTGATGCAAATAGAGACGAAAAAGAAGAGGTGTCTTTGGATTCTCCTATTATAAGAACAAATGATGATCAAAACAGAGCAGAAACAGAGGATTCCGATCAAAACAGAACAGAAAGAGAGGATTCCGGTCAAAACAGAGCAGAAACGAAGAATTCCGATCAAAACAGAGCAGAAACAGAGGGTTCCGATCAAAACAGAGTAGAAACAGAGGATTTCGATCAAAACAGAGCAGAAACGGATAATTCAGATCAAAACAGAGCAGAAACGGAGAATTCCTATCAAAACAGAGCAGAAACGGAGAATTCCGATCAAAACAGAGCAGAAACACAGAATTCCGGTTTTTTGGAGACCGTCTCTGCTGTAACCACCGATTTGTATGAGTATACTTCTAATAGAGTTATCAATGGCTATATGGAAGAACCAAAAGCCACGAGCTTTATTGTCCAAGAATTCTATGTTGATTGGGGCAGTTCTAAATTCTCTGATTATCAAATTCCTATTAAAGCAGCAGAGGATGATGATGCTGATCAGGAAAAAGTAGTGGATTATATTGAAAGCGTTGAGGCCCTAGAGAATGGAGAGCACAAGAAACCAGTCAGTGATCAAAACCCTCTTGAGGAATTCAGTACTGGGAAGGTTTTAGATGCTGAAATTGAGCTTTGTGAGGTTGAAGAAGACAATTTCTCTGTAGTTTCTGATTCGAAACAAGACCCAGTTAGATCAAGAAAAGATCATGATGTGGGTTCACTTAACTACGAGTTTTTGATATACAGAAATGTGGTTCAGTTTGATGAGGGACTTGACCCTCAAAGCTCTGGATTCCAGTATGATGAGGAAGATCATAGAGTCCACTTTGATATGGAAGATGATCAATTGCTTGATGTAAGTATCCCAGATGGAAAATCAGTTGTCTTTTCCGATCAACTCGATGGTTCTGAATCTCCTGATGATGAATACATTGAATTGTTAGAACCCCAATTGAGAAATTATAGTCAACGACTTGATCAAGTTGTGGAGAGAGGTGAAGATAAAGAGGAACTAGTCCAAGTTGAGACAGAAGCCACGAGTCCACTAGTACAATCTGCACAAGAAGAAATAGGATTGCACCCTAAAAAGTTTGGATTGTATTCGGATGATGAAAACGAATTGGAGTCTCCTGACGAGCATGATGATCTAATTGAACAGATTAAAAGGGAAATTAAGAATGTGAGAACCGGAGGGCTTCCCACTATTTCAGAAGAAGATCATTCGGAGTCTACAAGAATGGTGGAGAATCTAAAGCTTAAGCCACTGAAGATTGATGAAAAGCTCGAATACAAAGATCGAATCGCAGAGATTCAGAAGGTGTACAAAAGCTACGCAGAGAAAATGCGAAAGCTGGACATCTTGAACAACCAAACCATGCATGCAATTGGTTTTCTACAGCTGAAAGACCAAGTTAAGCCAATTTCATTGGAAAAGTCTTCCATTAATCCAATTATGAAGTCTCTTCTTTCGCAGAACCTGTTGAGGTGTAAAGCACAAAGACGACCGACAGTTGACCCTATGCCGAAATTTGAGGGAGACTTGCACAAGGACTTGGAATTGGTGTATGTTGGACAGGTTTGCCTCTCTTGGGAAATTCTTCACTGGCAGCACAGAAAAGCCCAAGAGTTGCAGCAATATGATCATCATCACTATCATGTTGTTGCCACTGAGTTTCAACTGTTTCAAGTGCTCCTGCAAAGATTCATAGAAGACGATCCATTTCACGGCCCTCGAGTCCAGCACTATGTCCGGAACCGATGTGTCCTTCGTAGCCTTCTTCAAGTTCCAGCCATCAGAGATGATTGCTGGAAGGATAAGAAGCATAACATGGGAGGGGAGGAGGATTTGATAATCTTAAGTGAGATGCTGGTGAAAATCATTGAAGAAACGATGCAGATATTTTGGAAATTTCTTCGCAAGGATGAGAGAAATGCGGTCCTAAAGAGCCTTCAAGAAAGTCAGGTTGAGCATAAGGATTTGGAGCTTCTTGTAGATATCCAGAGAGATCTTCAAAAGAAGGAGAAAAAGCTCAAAGACATACAGAGAAGTGGAAATTGTGTAGTGAAGAAATTCCAAAAGCAGCATCAAGATCAAGACAGACTCGAGCATAGTTTGTTTGTCGCTCAAGTTGAACTGAGGTTGGTTTCAAGAGTGCTCAACATGTCAAAACTAACATCAGATCAGTTAGTTTGGTGTCATGAAAAGCTAGACAACATCAATTTTGTTCACAGAAAGGTTCTTATGGAGCCTTCGTTTCTTCTTTTTCCATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1666

Protein Analysis

799

Amino Acids

93.58

Weight (kDa)

4.75

Isoelectric Point (pI)

46.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1666 PF07891 558 - 798 1.6e-88 Protein of unknown function (DUF1666)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014609)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G69610 AT1G69610 AT1G69610
fragaria_vesca FvH4_4g31360
malus_domestica MD16G1072900.v1.1
prunus_persica Prupe.1G274600_v2.0.a1
pyrus_communis pycom16g06320
rosa_chinensis RchiOBHm_Chr4g0439871
rosa_laevigata RLG00000006231
rosa_multiflora Rmu_sc0000497.1_g000007
rosa_roxburghii Rroxscaffold_5G00380780
rosa_rugosa Rorug04G0321800
rosa_samantha Rh4AG372900 Rh4BG385300 Rh4CG399800 Rh4DG379500
rosa_wichuraiana Rw4G031880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 152
AccI GTMKAC 2 cut(s) 482, 1355
AccIII TCCGGA 1 cut(s) 1875
AciI CCGC 1 cut(s) 2060
AclI AACGTT 1 cut(s) 6
AclWI GGATC 1 cut(s) 1835
AcuI CTGAAG 1 cut(s) 1412
AfaI GTAC 3 cut(s) 727, 1176, 1448
AfiI CCNNNNNNNGG 3 cut(s) 715, 1714, 1850
AflII CTTAAG 2 cut(s) 1382, 1981
AflIII ACRYGT 1 cut(s) 2286
AhlI ACTAGT 2 cut(s) 1138, 1171
Alw21I GWGCWC 3 cut(s) 684, 1818, 2283
Alw26I GTCTC 7 cut(s) 108, 440, 457, 1145, 1257, 1590, 1656
AlwI GGATC 1 cut(s) 1835
AlwNI CAGNNNCTG 1 cut(s) 1603
Ama87I CYCGRG 2 cut(s) 1857, 2228
Aor13HI TCCGGA 1 cut(s) 1875
AoxI GGCC 2 cut(s) 663, 1852
ApeKI GCWGC 4 cut(s) 605, 1732, 1756, 2206
AseI ATTAAT 1 cut(s) 1569
Asp700I GAANNNNTTC 2 cut(s) 1722, 2195
AspS9I GGNCC 3 cut(s) 664, 1853, 2062
AsuHPI GGTGA 2 cut(s) 1133, 2008
AsuII TTCGAA 1 cut(s) 800
AvaI CYCGRG 2 cut(s) 1857, 2228
AvaII GGWCC 1 cut(s) 2062
BauI CACGAG 2 cut(s) 529, 1162
BbsI GAAGAC 3 cut(s) 780, 1554, 1842
Bbv12I GWGCWC 3 cut(s) 684, 1818, 2283
BbvI GCAGC 4 cut(s) 617, 1744, 1768, 2218
BccI CCATC 3 cut(s) 989, 1022, 1925
BceAI ACGGC 1 cut(s) 1867
BciVI GTATCC 1 cut(s) 998
BclI TGATCA 7 cut(s) 91, 166, 625, 700, 967, 1102, 1765
BcoDI GTCTC 7 cut(s) 108, 440, 457, 1145, 1257, 1590, 1656
BcuI ACTAGT 2 cut(s) 1138, 1171
BfaI CTAG 4 cut(s) 668, 1139, 1172, 2333
BfmI CTRYAG 2 cut(s) 786, 1520
BfrI CTTAAG 2 cut(s) 1382, 1981
BfuI GTATCC 1 cut(s) 998
BglII AGATCT 1 cut(s) 2131
BisI GCNGC 4 cut(s) 606, 1733, 1757, 2207
BlsI GCNGC 4 cut(s) 607, 1734, 1758, 2208
BmcAI AGTACT 1 cut(s) 727
Bme18I GGWCC 1 cut(s) 2062
BmeT110I CYCGRG 2 cut(s) 1857, 2228
BmgT120I GGNCC 3 cut(s) 664, 1853, 2062
BmiI GGNNCC 3 cut(s) 283, 1880, 2373
BmrI ACTGGG 2 cut(s) 738, 806
BmsI GCATC 6 cut(s) 94, 610, 733, 1980, 2007, 2218
BmuI ACTGGG 2 cut(s) 738, 806
BpiI GAAGAC 3 cut(s) 780, 1554, 1842
BplI GAGNNNNNCTC 2 cut(s) 437, 469
Bpu14I TTCGAA 1 cut(s) 800
BpuEI CTTGAG 2 cut(s) 734, 2235
BsaBI GATNNNNATC 4 cut(s) 195, 315, 624, 1770
BsaI GGTCTC 1 cut(s) 440
BsaWI WCCGGW 4 cut(s) 224, 434, 1313, 1875
BsaXI ACNNNNNCTCC 2 cut(s) 2363, 2393
Bsc4I CCNNNNNNNGG 3 cut(s) 715, 1714, 1850
Bse1I ACTGG 5 cut(s) 692, 733, 812, 922, 1733
Bse8I GATNNNNATC 4 cut(s) 195, 315, 624, 1770
BseAI TCCGGA 1 cut(s) 1875
BseGI GGATG 3 cut(s) 619, 1237, 2053
BseJI GATNNNNATC 4 cut(s) 195, 315, 624, 1770
BseLI CCNNNNNNNGG 3 cut(s) 715, 1714, 1850
BseMII CTCAG 4 cut(s) 49, 70, 1785, 2252
BseNI ACTGG 5 cut(s) 692, 733, 812, 922, 1733
BseRI GAGGAG 1 cut(s) 1979
BseXI GCAGC 4 cut(s) 617, 1744, 1768, 2218
BsgI GTGCAG 1 cut(s) 1167
Bsh1285I CGRYCG 1 cut(s) 1631
BshFI GGCC 2 cut(s) 665, 1854
BsiEI CGRYCG 1 cut(s) 1631
BsiHKAI GWGCWC 3 cut(s) 684, 1818, 2283
BsiHKCI CYCGRG 2 cut(s) 1857, 2228
BsiSI CCGG 4 cut(s) 225, 435, 1314, 1876
BslFI GGGAC 1 cut(s) 909
BslI CCNNNNNNNGG 3 cut(s) 715, 1714, 1850
BsmAI GTCTC 7 cut(s) 108, 440, 457, 1145, 1257, 1590, 1656
BsmBI CGTCTC 2 cut(s) 108, 457
BsmFI GGGAC 1 cut(s) 909
BsnI GGCC 2 cut(s) 665, 1854
Bso31I GGTCTC 1 cut(s) 440
BsoBI CYCGRG 2 cut(s) 1857, 2228
Bsp119I TTCGAA 1 cut(s) 800
Bsp1286I GDGCHC 3 cut(s) 684, 1818, 2283
Bsp13I TCCGGA 1 cut(s) 1875
Bsp1407I TGTACA 1 cut(s) 1446
BspACI CCGC 1 cut(s) 2060
BspANI GGCC 2 cut(s) 665, 1854
BspCNI CTCAG 4 cut(s) 48, 69, 1786, 2253
BspEI TCCGGA 1 cut(s) 1875
BspHI TCATGA 2 cut(s) 832, 2323
BspLI GGNNCC 3 cut(s) 283, 1880, 2373
BspPI GGATC 1 cut(s) 1835
BspT104I TTCGAA 1 cut(s) 800
BspTI CTTAAG 2 cut(s) 1382, 1981
BspTNI GGTCTC 1 cut(s) 440
BsrGI TGTACA 1 cut(s) 1446
BsrI ACTGG 5 cut(s) 692, 733, 812, 922, 1733
BssNAI GTATAC 1 cut(s) 483
BssSI CACGAG 2 cut(s) 529, 1162
Bst1107I GTATAC 1 cut(s) 483
Bst2BI CACGAG 2 cut(s) 529, 1162
Bst4CI ACNGT 3 cut(s) 451, 1636, 1806
Bst6I CTCTTC 2 cut(s) 120, 1593
BstAFI CTTAAG 2 cut(s) 1382, 1981
BstAUI TGTACA 1 cut(s) 1446
BstBI TTCGAA 1 cut(s) 800
BstC8I GCNNGC 1 cut(s) 1506
BstDEI CTNAG 4 cut(s) 35, 56, 1794, 2261
BstF5I GGATG 3 cut(s) 619, 1237, 2053
BstMAI GTCTC 7 cut(s) 108, 440, 457, 1145, 1257, 1590, 1656
BstMCI CGRYCG 1 cut(s) 1631
BstNSI RCATGY 2 cut(s) 1508, 2290
BstSFI CTRYAG 2 cut(s) 786, 1520
BstV1I GCAGC 4 cut(s) 617, 1744, 1768, 2218
BstV2I GAAGAC 3 cut(s) 780, 1554, 1842
BstX2I RGATCY 1 cut(s) 2131
BstYI RGATCY 1 cut(s) 2131
BstZ17I GTATAC 1 cut(s) 483
BsuI GTATCC 1 cut(s) 998
BsuRI GGCC 2 cut(s) 665, 1854
BtsCI GGATG 3 cut(s) 619, 1237, 2053
BtsIMutI CAGTG 4 cut(s) 703, 1388, 1726, 1791
Cac8I GCNNGC 1 cut(s) 1506
CaiI CAGNNNCTG 1 cut(s) 1603
CciI TCATGA 2 cut(s) 832, 2323
Cfr13I GGNCC 3 cut(s) 664, 1853, 2062
Csp6I GTAC 3 cut(s) 726, 1175, 1447
CspCI CAANNNNNGTGG 2 cut(s) 1347, 1382
CviAII CATG 9 cut(s) 833, 1265, 1501, 1505, 1781, 1954, 2287, 2324, 2394
CviQI GTAC 3 cut(s) 726, 1175, 1447
DdeI CTNAG 4 cut(s) 35, 56, 1794, 2261
Eam1104I CTCTTC 2 cut(s) 120, 1593
EarI CTCTTC 2 cut(s) 120, 1593
Eco31I GGTCTC 1 cut(s) 440
Eco32I GATATC 1 cut(s) 2122
Eco47I GGWCC 1 cut(s) 2062
Eco57I CTGAAG 1 cut(s) 1412
Eco88I CYCGRG 2 cut(s) 1857, 2228
EcoO109I RGGNCCY 1 cut(s) 664
EcoRI GAATTC 6 cut(s) 250, 370, 400, 430, 548, 719
EcoRV GATATC 1 cut(s) 2122
EcoT22I ATGCAT 1 cut(s) 1506
Esp3I CGTCTC 2 cut(s) 108, 457
FaeI CATG 9 cut(s) 836, 1268, 1504, 1508, 1784, 1957, 2290, 2327, 2397
FaqI GGGAC 1 cut(s) 909
FatI CATG 9 cut(s) 832, 1264, 1500, 1504, 1780, 1953, 2286, 2323, 2393
FbaI TGATCA 7 cut(s) 91, 166, 625, 700, 967, 1102, 1765
FblI GTMKAC 2 cut(s) 482, 1355
Fnu4HI GCNGC 4 cut(s) 606, 1733, 1757, 2207
FokI GGATG 3 cut(s) 626, 1244, 2060
Fsp4HI GCNGC 4 cut(s) 606, 1733, 1757, 2207
FspBI CTAG 4 cut(s) 668, 1139, 1172, 2333
GluI GCNGC 4 cut(s) 606, 1733, 1757, 2207
HaeIII GGCC 2 cut(s) 665, 1854
HapII CCGG 4 cut(s) 225, 435, 1314, 1876
Hin1II CATG 9 cut(s) 836, 1268, 1504, 1508, 1784, 1957, 2290, 2327, 2397
HincII GTYRAC 2 cut(s) 1094, 1639
HindII GTYRAC 2 cut(s) 1094, 1639
HindIII AAGCTT 1 cut(s) 1379
HpaII CCGG 4 cut(s) 225, 435, 1314, 1876
HphI GGTGA 2 cut(s) 1133, 2008
Hpy166II GTNNAC 9 cut(s) 483, 845, 949, 1094, 1169, 1356, 1447, 1639, 2353
Hpy8I GTNNAC 9 cut(s) 483, 845, 949, 1094, 1169, 1356, 1447, 1639, 2353
HpyAV CCTTC 8 cut(s) 727, 1435, 1901, 1910, 1930, 2084, 2137, 2385
HpyCH4III ACNGT 3 cut(s) 451, 1636, 1806
HpyCH4IV ACGT 1 cut(s) 6
HpyCH4V TGCA 9 cut(s) 107, 1184, 1204, 1504, 1508, 1669, 1756, 1822, 2020
HpyF3I CTNAG 4 cut(s) 35, 56, 1794, 2261
HpySE526I ACGT 1 cut(s) 6
Hsp92II CATG 9 cut(s) 836, 1268, 1504, 1508, 1784, 1957, 2290, 2327, 2397
Kpn2I TCCGGA 1 cut(s) 1875
Ksp22I TGATCA 7 cut(s) 91, 166, 625, 700, 967, 1102, 1765
LmnI GCTCC 4 cut(s) 85, 1821, 2107, 2371
Lsp1109I GCAGC 4 cut(s) 617, 1744, 1768, 2218
LweI GCATC 6 cut(s) 94, 610, 733, 1980, 2007, 2218
MaeI CTAG 4 cut(s) 668, 1139, 1172, 2333
MaeII ACGT 1 cut(s) 6
MaeIII GTNAC 1 cut(s) 460
MfeI CAATTG 3 cut(s) 971, 1076, 1509
MflI RGATCY 1 cut(s) 2131
MhlI GDGCHC 3 cut(s) 684, 1818, 2283
MlyI GAGTC 6 cut(s) 954, 1174, 1259, 1361, 1869, 2220
MmeI TCCRAC 1 cut(s) 1675
Mph1103I ATGCAT 1 cut(s) 1506
MroI TCCGGA 1 cut(s) 1875
MroXI GAANNNNTTC 2 cut(s) 1722, 2195
MseI TTAA 9 cut(s) 600, 849, 1287, 1299, 1383, 1539, 1569, 1982, 2398
MslI CAYNNNNRTG 1 cut(s) 1779
MspA1I CMGCKG 1 cut(s) 1525
MspCI CTTAAG 2 cut(s) 1382, 1981
MspI CCGG 4 cut(s) 225, 435, 1314, 1876
MunI CAATTG 3 cut(s) 971, 1076, 1509
NlaIII CATG 9 cut(s) 836, 1268, 1504, 1508, 1784, 1957, 2290, 2327, 2397
NlaIV GGNNCC 3 cut(s) 283, 1880, 2373
NsiI ATGCAT 1 cut(s) 1506
NspI RCATGY 2 cut(s) 1508, 2290
NspV TTCGAA 1 cut(s) 800
PaeI GCATGC 1 cut(s) 1508
PaeR7I CTCGAG 2 cut(s) 1857, 2228
PagI TCATGA 2 cut(s) 832, 2323
PciI ACATGT 1 cut(s) 2286
PdmI GAANNNNTTC 2 cut(s) 1722, 2195
PkrI GCNGC 4 cut(s) 607, 1734, 1758, 2208
PleI GAGTC 6 cut(s) 953, 1173, 1258, 1360, 1868, 2220
PpsI GAGTC 6 cut(s) 953, 1173, 1258, 1360, 1868, 2220
PscI ACATGT 1 cut(s) 2286
PshBI ATTAAT 1 cut(s) 1569
PsiI TTATAA 1 cut(s) 152
Psp1406I AACGTT 1 cut(s) 6
PspN4I GGNNCC 3 cut(s) 283, 1880, 2373
PspPI GGNCC 3 cut(s) 664, 1853, 2062
PspXI VCTCGAGB 2 cut(s) 1857, 2228
PstNI CAGNNNCTG 1 cut(s) 1603
PsuI RGATCY 1 cut(s) 2131
PvuII CAGCTG 1 cut(s) 1525
RsaI GTAC 3 cut(s) 727, 1176, 1448
RsaNI GTAC 3 cut(s) 726, 1175, 1447
RseI CAYNNNNRTG 1 cut(s) 1779
SaqAI TTAA 9 cut(s) 600, 849, 1287, 1299, 1383, 1539, 1569, 1982, 2398
SatI GCNGC 4 cut(s) 606, 1733, 1757, 2207
Sau96I GGNCC 3 cut(s) 664, 1853, 2062
ScaI AGTACT 1 cut(s) 727
SchI GAGTC 6 cut(s) 954, 1174, 1259, 1361, 1869, 2220
SduI GDGCHC 3 cut(s) 684, 1818, 2283
SfaNI GCATC 6 cut(s) 94, 610, 733, 1980, 2007, 2218
SfcI CTRYAG 2 cut(s) 786, 1520
Sfr274I CTCGAG 2 cut(s) 1857, 2228
SfuI TTCGAA 1 cut(s) 800
SinI GGWCC 1 cut(s) 2062
SlaI CTCGAG 2 cut(s) 1857, 2228
SmiMI CAYNNNNRTG 1 cut(s) 1779
SmlI CTYRAG 6 cut(s) 713, 1382, 1857, 1981, 2228, 2250
SmoI CTYRAG 6 cut(s) 713, 1382, 1857, 1981, 2228, 2250
SpeI ACTAGT 2 cut(s) 1138, 1171
SphI GCATGC 1 cut(s) 1508
SsiI CCGC 1 cut(s) 2060
SspMI CTAG 4 cut(s) 668, 1139, 1172, 2333
TaaI ACNGT 3 cut(s) 451, 1636, 1806
TaiI ACGT 1 cut(s) 9
TaqI TCGA 7 cut(s) 315, 800, 1026, 1410, 1423, 1858, 2229
TaqII GACCGA 1 cut(s) 1645
TatI WGTACW 3 cut(s) 725, 1174, 1446
Tru1I TTAA 9 cut(s) 600, 849, 1287, 1299, 1383, 1539, 1569, 1982, 2398
Tru9I TTAA 9 cut(s) 600, 849, 1287, 1299, 1383, 1539, 1569, 1982, 2398
TscAI CASTG 4 cut(s) 703, 1395, 1733, 1798
TseI GCWGC 4 cut(s) 605, 1732, 1756, 2206
TspDTI ATGAA 8 cut(s) 17, 1065, 1251, 1416, 1539, 1595, 1819, 2340
TspGWI ACGGA 3 cut(s) 350, 380, 410
TspRI CASTG 4 cut(s) 703, 1395, 1733, 1798
Vha464I CTTAAG 2 cut(s) 1382, 1981
VpaK11BI GGWCC 1 cut(s) 2062
VspI ATTAAT 1 cut(s) 1569
XceI RCATGY 2 cut(s) 1508, 2290
XhoI CTCGAG 2 cut(s) 1857, 2228
XmiI GTMKAC 2 cut(s) 482, 1355
XmnI GAANNNNTTC 2 cut(s) 1722, 2195
XspI CTAG 4 cut(s) 668, 1139, 1172, 2333
ZrmI AGTACT 1 cut(s) 727
Zsp2I ATGCAT 1 cut(s) 1506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.