RLG00000006231

Protein of unknown function (DUF1666)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0014609
Physical Location & Seq
Forward (+)
5396420 .. 5400174
3755 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006231

Sequence Viewer

Length: 2523 bp
ATGGTTATTACGAAATTCATGGTGCTGCAAACCCAGTTTGATTTTATTAGCAAACGTGTGTCAAGCGGAAACATGCTTGGAGTCCTCAGTTCCATTTGGGTTTTTGTCTGGAACTACTTGTTCTTTGTGTTTGGATTCATTAGTAGATACATACTTATCAGATTTCAGGCAGATACTCTCAGAAATCATAACCCTGTTGTTTGTTTGGAGCGTGATCATCAATTTGATGCAAATAGAGACGAAAAAGAAGAGGTGTCTTTGGATTCTCCTATTATAAGTAGAAATGATCATCAAAACAGAACAGAAAGAGAGGATTCCGGTCAAAACAGAGCAGAAACGAAGAATTCCGATCAAAACAGAGCAGAAACAGGGGGTTCCGATCAAAACAGAGCAGAAACAGAGGATTCCCATCAAAACAGAGCAGAAACGGATAATTCAGATGAAAACAGAGCAGAAACAGAGGATTCCGGTCAAAACAGAGCAGAAACGAAGAATTCGGATCAAAACAGAGCAGAAACAGAGAATTCCGGTTTTGTGGAGACCGTCTCTGCTGTAACCACCGATTTGTATGAGTATACTTCTAATAGAGTTATCAATGGCTATATGGAAGAACCAAAAACCACGAGCTTTATTGTCCAAGAATTCTATGTTGATTGGGGCAGTTCTAAATTCTCTGATTATCAAATTCCTATTAAAGCAGCAAAGGATGATGATGCTGATCATGAAAAAGTAGTGGATTCTATTGAAAGCGTTGAGGCCTTAGAGAATGGAGAGCACGAGAAACCAGTCACTGATCAAAACCCTCTTGAGGAATTCAGTACTGGGAAGGTTTTAGATGCTGAAATTGAGCTTTGTGAGGTTGAAGAAGACAATTTCTCTGTAGTTTCAGATTCGAAACAAGACCCAGTTAGCTCAAGAAAAGATCATGATGTGGGTTCACTTAACTACGAGTTTTTGATATACAGAAATGTGGTTCAGTTTGATGAGGGACTTGACCCTCAAAGCTCTGGATTCCAGTATGATGAGGAAGATCATAGAGTCCACTTTGATACGGAAGATGATCAATTGCTTGATGTAAGTATCCCAGAGGGAAAAGCAGTTGTCTTTTCCGATCAACTCGATGGTTCTGAATCTCCTGATGATGAATACATTGAATTGTTAGAGCCCCAATTGAGAAGTTATAGTCAACGACTTGATCAAGTTGTGGAGAGAGGTGAAGATAAAGAGGAACTAGTCCAAGTTGAGACAGAAGCCACGAGTCCGCTAGTACAATCCGCACAAGAAGAAATAGGATTGCAGCCTAAAAAGTCTGGATTGTATTCGGATGATGAAAACGAATTGGAGTCTCCTGACGAGCATGATGATCTAATTGAACAGATTAAAAGGGAAATTAAGAATGTGAGAACCGGAGGGCTTCCCACTATTTCAGAAGAAGATCATTCGGAGTCTACAAGAATGGTGGAGAATCTGACGCTTAAGCCACTGAAGATTGATGAAAAGCTCGAATACAAAGATCGAATCGCAGAGATTCAGAAGGTGTACAAAAGCTACGCAGAGAAAATGCGAAAGCTGGACATCTTGAACAACCAGACCATGCATGCAATTGGTTTTCTACAGCTGAAAGACCAAGTTAAGCCAATTTCATTGGAAAAGTCTTCCATTAATCCAATTATGAAGTCTCTTCTTTCGCAGAACCTGTTGAGGTGCAAAGCACAAAGACGACCGACAGTTGACCCTATGCCGAAATTTGTGGGAGACTTGCACAAGGACTTGGAATTGGTCTATGTTGGACAGGTTTGCCTCTCTTGGGAAATTCTTCACTGGCAGCACAGAAAAGCCCAAGAGTTGCAGCAATATGATCATCATCACTATGATGTTGTTGCCACTGAGTTTCAACTGTTTCAAGTGCTCCTGCAAAGATTCATAGAAGACGATCCATTTCAAGGCCCTCGAGTCCAGCACTATGTCCGGAACCGATGTGTCCTTCGTAGTCTTCTTCAAGTTCCAGCCATCAGAGATGATTGCAGGAAGGATAAGAAGCATGACATGGGAGGGGAGGAGGATTTGATAATCTCAAGTGAGATGCTGGTGAAAATCATTGAGGAAACGATGCAGATATTTTGGAAATTTCTTCGCAAGGATGAGAGAAATGCGGTCCTAAAGAGCCTTCAAGAAAGTCAGGTTGAGCATAAGGACTTGGAGCTTCTTGTAGATATCCGGAGGGATCTTCAAAAGAAGGAGAAAAAGCTCAAAGACATACAGAGGAGTGGAAATTGTGTAGTGAAGAAGTTCCAAAAGCAGCATCAAGATCAAGACAGACTCGAGCATAGTTTTTTTGTCGCTCAAGTTGAACTGAGGTTGGTTTCAAGAGTGCTCAACATGTCAAAACTAACATCAGATCAGTTAGTTTGGTGCCATGAAAAGCTAGACAACATCAATTTTGTTCACAGAAAGATCACAGATGCTGCAATAAGAGAAGGATGGTCAATGATCTTTGTAAATCTATCAAGGACTAACACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1666

Protein Analysis

841

Amino Acids

97.6

Weight (kDa)

4.86

Isoelectric Point (pI)

45.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1666 PF07891 589 - 821 1.1e-84 Protein of unknown function (DUF1666)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014609)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G69610 AT1G69610 AT1G69610
fragaria_vesca FvH4_4g31360
malus_domestica MD16G1072900.v1.1
prunus_persica Prupe.1G274600_v2.0.a1
pyrus_communis pycom16g06320
rosa_chinensis RchiOBHm_Chr4g0439871
rosa_laevigata RLG00000006231
rosa_multiflora Rmu_sc0000497.1_g000007
rosa_roxburghii Rroxscaffold_5G00380780
rosa_rugosa Rorug04G0321800
rosa_samantha Rh4AG372900 Rh4BG385300 Rh4CG399800 Rh4DG379500
rosa_wichuraiana Rw4G031880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 275
AccB1I GGYRCC 1 cut(s) 2412
AccI GTMKAC 2 cut(s) 575, 1448
AccIII TCCGGA 2 cut(s) 1968, 2217
AciI CCGC 4 cut(s) 66, 1262, 1275, 2153
AclWI GGATC 3 cut(s) 507, 1928, 2232
AcuI CTGAAG 1 cut(s) 1505
AfaI GTAC 3 cut(s) 820, 1269, 1541
AfiI CCNNNNNNNGG 3 cut(s) 808, 1807, 1943
AflII CTTAAG 1 cut(s) 1475
AflIII ACRYGT 2 cut(s) 55, 2379
AhlI ACTAGT 1 cut(s) 1231
Alw21I GWGCWC 3 cut(s) 777, 1911, 2376
Alw26I GTCTC 7 cut(s) 231, 533, 550, 1238, 1350, 1683, 1749
AlwI GGATC 3 cut(s) 507, 1928, 2232
AlwNI CAGNNNCTG 3 cut(s) 791, 1696, 2465
Ama87I CYCGRG 2 cut(s) 1950, 2321
Aor13HI TCCGGA 2 cut(s) 1968, 2217
AoxI GGCC 2 cut(s) 756, 1945
ApeKI GCWGC 7 cut(s) 25, 698, 1297, 1825, 1849, 2299, 2465
AseI ATTAAT 1 cut(s) 1662
Asp700I GAANNNNTTC 2 cut(s) 1815, 2288
AspS9I GGNCC 2 cut(s) 1946, 2155
AsuHPI GGTGA 2 cut(s) 1226, 2101
AsuII TTCGAA 1 cut(s) 893
AvaI CYCGRG 2 cut(s) 1950, 2321
AvaII GGWCC 1 cut(s) 2155
BanI GGYRCC 1 cut(s) 2412
BanII GRGCYC 1 cut(s) 1167
BauI CACGAG 3 cut(s) 622, 776, 1255
BbsI GAAGAC 4 cut(s) 873, 1647, 1935, 1985
Bbv12I GWGCWC 3 cut(s) 777, 1911, 2376
BbvI GCAGC 7 cut(s) 12, 710, 1309, 1837, 1861, 2311, 2452
BccI CCATC 4 cut(s) 417, 1115, 2018, 2475
BciVI GTATCC 1 cut(s) 1091
BclI TGATCA 7 cut(s) 214, 286, 718, 793, 1060, 1195, 1858
BcoDI GTCTC 7 cut(s) 231, 533, 550, 1238, 1350, 1683, 1749
BcuI ACTAGT 1 cut(s) 1231
BfaI CTAG 3 cut(s) 1232, 1265, 2426
BfmI CTRYAG 2 cut(s) 879, 1613
BfrI CTTAAG 1 cut(s) 1475
BfuI GTATCC 1 cut(s) 1091
BisI GCNGC 7 cut(s) 26, 699, 1298, 1826, 1850, 2300, 2466
BlsI GCNGC 7 cut(s) 27, 700, 1299, 1827, 1851, 2301, 2467
BmcAI AGTACT 1 cut(s) 820
Bme18I GGWCC 1 cut(s) 2155
BmeT110I CYCGRG 2 cut(s) 1950, 2321
BmgT120I GGNCC 2 cut(s) 1946, 2155
BmiI GGNNCC 3 cut(s) 376, 1973, 2414
BmrI ACTGGG 3 cut(s) 28, 831, 899
BmsI GCATC 7 cut(s) 217, 703, 826, 2073, 2100, 2311, 2452
BmuI ACTGGG 3 cut(s) 28, 831, 899
BpiI GAAGAC 4 cut(s) 873, 1647, 1935, 1985
BplI GAGNNNNNCTC 2 cut(s) 530, 562
Bpu14I TTCGAA 1 cut(s) 893
BpuEI CTTGAG 4 cut(s) 827, 898, 2059, 2328
BsaBI GATNNNNATC 3 cut(s) 408, 717, 1863
BsaI GGTCTC 1 cut(s) 533
BsaWI WCCGGW 6 cut(s) 317, 467, 527, 1406, 1968, 2217
Bsc4I CCNNNNNNNGG 3 cut(s) 808, 1807, 1943
Bse1I ACTGG 6 cut(s) 34, 785, 826, 905, 1015, 1826
Bse8I GATNNNNATC 3 cut(s) 408, 717, 1863
BseAI TCCGGA 2 cut(s) 1968, 2217
BseGI GGATG 4 cut(s) 712, 1330, 2146, 2486
BseJI GATNNNNATC 3 cut(s) 408, 717, 1863
BseLI CCNNNNNNNGG 3 cut(s) 808, 1807, 1943
BseMII CTCAG 4 cut(s) 100, 193, 1878, 2345
BseNI ACTGG 6 cut(s) 34, 785, 826, 905, 1015, 1826
BseRI GAGGAG 2 cut(s) 2072, 2278
BseXI GCAGC 7 cut(s) 12, 710, 1309, 1837, 1861, 2311, 2452
Bsh1285I CGRYCG 1 cut(s) 1724
BshFI GGCC 2 cut(s) 758, 1947
BshNI GGYRCC 1 cut(s) 2412
BsiEI CGRYCG 1 cut(s) 1724
BsiHKAI GWGCWC 3 cut(s) 777, 1911, 2376
BsiHKCI CYCGRG 2 cut(s) 1950, 2321
BsiSI CCGG 6 cut(s) 318, 468, 528, 1407, 1969, 2218
BslFI GGGAC 1 cut(s) 1002
BslI CCNNNNNNNGG 3 cut(s) 808, 1807, 1943
BsmAI GTCTC 7 cut(s) 231, 533, 550, 1238, 1350, 1683, 1749
BsmBI CGTCTC 2 cut(s) 231, 550
BsmFI GGGAC 1 cut(s) 1002
BsnI GGCC 2 cut(s) 758, 1947
Bso31I GGTCTC 1 cut(s) 533
BsoBI CYCGRG 2 cut(s) 1950, 2321
Bsp119I TTCGAA 1 cut(s) 893
Bsp1286I GDGCHC 4 cut(s) 777, 1167, 1911, 2376
Bsp13I TCCGGA 2 cut(s) 1968, 2217
Bsp1407I TGTACA 1 cut(s) 1539
BspACI CCGC 4 cut(s) 66, 1262, 1275, 2153
BspANI GGCC 2 cut(s) 758, 1947
BspCNI CTCAG 4 cut(s) 99, 192, 1879, 2346
BspEI TCCGGA 2 cut(s) 1968, 2217
BspHI TCATGA 2 cut(s) 721, 925
BspLI GGNNCC 3 cut(s) 376, 1973, 2414
BspPI GGATC 3 cut(s) 507, 1928, 2232
BspT104I TTCGAA 1 cut(s) 893
BspT107I GGYRCC 1 cut(s) 2412
BspTI CTTAAG 1 cut(s) 1475
BspTNI GGTCTC 1 cut(s) 533
BsrGI TGTACA 1 cut(s) 1539
BsrI ACTGG 6 cut(s) 34, 785, 826, 905, 1015, 1826
BssNAI GTATAC 1 cut(s) 576
BssSI CACGAG 3 cut(s) 622, 776, 1255
Bst1107I GTATAC 1 cut(s) 576
Bst2BI CACGAG 3 cut(s) 622, 776, 1255
Bst4CI ACNGT 3 cut(s) 544, 1729, 1899
Bst6I CTCTTC 2 cut(s) 243, 1686
BstAFI CTTAAG 1 cut(s) 1475
BstAUI TGTACA 1 cut(s) 1539
BstBI TTCGAA 1 cut(s) 893
BstC8I GCNNGC 1 cut(s) 1599
BstDEI CTNAG 5 cut(s) 86, 179, 760, 1887, 2354
BstF5I GGATG 4 cut(s) 712, 1330, 2146, 2486
BstMAI GTCTC 7 cut(s) 231, 533, 550, 1238, 1350, 1683, 1749
BstMCI CGRYCG 1 cut(s) 1724
BstNSI RCATGY 3 cut(s) 76, 1601, 2383
BstSFI CTRYAG 2 cut(s) 879, 1613
BstV1I GCAGC 7 cut(s) 12, 710, 1309, 1837, 1861, 2311, 2452
BstV2I GAAGAC 4 cut(s) 873, 1647, 1935, 1985
BstX2I RGATCY 1 cut(s) 2224
BstYI RGATCY 1 cut(s) 2224
BstZ17I GTATAC 1 cut(s) 576
BsuI GTATCC 1 cut(s) 1091
BsuRI GGCC 2 cut(s) 758, 1947
BtsCI GGATG 4 cut(s) 712, 1330, 2146, 2486
BtsIMutI CAGTG 4 cut(s) 789, 1481, 1819, 1884
Cac8I GCNNGC 1 cut(s) 1599
CaiI CAGNNNCTG 3 cut(s) 791, 1696, 2465
CciI TCATGA 2 cut(s) 721, 925
Cfr13I GGNCC 2 cut(s) 1946, 2155
CseI GACGC 1 cut(s) 1480
Csp6I GTAC 3 cut(s) 819, 1268, 1540
CspCI CAANNNNNGTGG 2 cut(s) 1440, 1475
CviQI GTAC 3 cut(s) 819, 1268, 1540
DdeI CTNAG 5 cut(s) 86, 179, 760, 1887, 2354
Eam1104I CTCTTC 2 cut(s) 243, 1686
EarI CTCTTC 2 cut(s) 243, 1686
Eco147I AGGCCT 1 cut(s) 758
Eco24I GRGCYC 1 cut(s) 1167
Eco31I GGTCTC 1 cut(s) 533
Eco32I GATATC 1 cut(s) 2215
Eco47I GGWCC 1 cut(s) 2155
Eco57I CTGAAG 1 cut(s) 1505
Eco88I CYCGRG 2 cut(s) 1950, 2321
EcoO109I RGGNCCY 1 cut(s) 1946
EcoRI GAATTC 5 cut(s) 343, 493, 523, 641, 812
EcoRV GATATC 1 cut(s) 2215
EcoT22I ATGCAT 1 cut(s) 1599
EcoT38I GRGCYC 1 cut(s) 1167
Esp3I CGTCTC 2 cut(s) 231, 550
FaqI GGGAC 1 cut(s) 1002
FbaI TGATCA 7 cut(s) 214, 286, 718, 793, 1060, 1195, 1858
FblI GTMKAC 2 cut(s) 575, 1448
Fnu4HI GCNGC 7 cut(s) 26, 699, 1298, 1826, 1850, 2300, 2466
FokI GGATG 4 cut(s) 719, 1337, 2153, 2493
FriOI GRGCYC 1 cut(s) 1167
Fsp4HI GCNGC 7 cut(s) 26, 699, 1298, 1826, 1850, 2300, 2466
FspBI CTAG 3 cut(s) 1232, 1265, 2426
GluI GCNGC 7 cut(s) 26, 699, 1298, 1826, 1850, 2300, 2466
HaeIII GGCC 2 cut(s) 758, 1947
HapII CCGG 6 cut(s) 318, 468, 528, 1407, 1969, 2218
HgaI GACGC 1 cut(s) 1480
HincII GTYRAC 2 cut(s) 1187, 1732
HindII GTYRAC 2 cut(s) 1187, 1732
HpaII CCGG 6 cut(s) 318, 468, 528, 1407, 1969, 2218
HphI GGTGA 2 cut(s) 1226, 2101
Hpy166II GTNNAC 8 cut(s) 576, 938, 1042, 1187, 1449, 1540, 1732, 2446
Hpy8I GTNNAC 8 cut(s) 576, 938, 1042, 1187, 1449, 1540, 1732, 2446
HpyAV CCTTC 7 cut(s) 820, 1528, 1994, 2023, 2177, 2230, 2471
HpyCH4III ACNGT 3 cut(s) 544, 1729, 1899
HpyCH4IV ACGT 1 cut(s) 55
HpyF3I CTNAG 5 cut(s) 86, 179, 760, 1887, 2354
HpySE526I ACGT 1 cut(s) 55
Kpn2I TCCGGA 2 cut(s) 1968, 2217
Ksp22I TGATCA 7 cut(s) 214, 286, 718, 793, 1060, 1195, 1858
LmnI GCTCC 3 cut(s) 208, 1914, 2200
Lsp1109I GCAGC 7 cut(s) 12, 710, 1309, 1837, 1861, 2311, 2452
LweI GCATC 7 cut(s) 217, 703, 826, 2073, 2100, 2311, 2452
MaeI CTAG 3 cut(s) 1232, 1265, 2426
MaeII ACGT 1 cut(s) 55
MaeIII GTNAC 2 cut(s) 553, 787
MfeI CAATTG 3 cut(s) 1064, 1169, 1602
MflI RGATCY 1 cut(s) 2224
MhlI GDGCHC 4 cut(s) 777, 1167, 1911, 2376
MlyI GAGTC 7 cut(s) 90, 1047, 1267, 1352, 1454, 1962, 2313
MmeI TCCRAC 1 cut(s) 1768
Mph1103I ATGCAT 1 cut(s) 1599
MroI TCCGGA 2 cut(s) 1968, 2217
MroXI GAANNNNTTC 2 cut(s) 1815, 2288
MseI TTAA 8 cut(s) 693, 942, 1380, 1392, 1476, 1632, 1662, 2521
MslI CAYNNNNRTG 2 cut(s) 1869, 1872
MspA1I CMGCKG 1 cut(s) 1618
MspCI CTTAAG 1 cut(s) 1475
MspI CCGG 6 cut(s) 318, 468, 528, 1407, 1969, 2218
MunI CAATTG 3 cut(s) 1064, 1169, 1602
NlaIV GGNNCC 3 cut(s) 376, 1973, 2414
NmuCI GTSAC 1 cut(s) 787
NsiI ATGCAT 1 cut(s) 1599
NspI RCATGY 3 cut(s) 76, 1601, 2383
NspV TTCGAA 1 cut(s) 893
PaeI GCATGC 1 cut(s) 1601
PaeR7I CTCGAG 2 cut(s) 1950, 2321
PagI TCATGA 2 cut(s) 721, 925
PceI AGGCCT 1 cut(s) 758
PciI ACATGT 1 cut(s) 2379
PdmI GAANNNNTTC 2 cut(s) 1815, 2288
PkrI GCNGC 7 cut(s) 27, 700, 1299, 1827, 1851, 2301, 2467
PleI GAGTC 7 cut(s) 89, 1046, 1266, 1351, 1453, 1961, 2313
PpsI GAGTC 7 cut(s) 89, 1046, 1266, 1351, 1453, 1961, 2313
PscI ACATGT 1 cut(s) 2379
PshBI ATTAAT 1 cut(s) 1662
PsiI TTATAA 1 cut(s) 275
PspN4I GGNNCC 3 cut(s) 376, 1973, 2414
PspPI GGNCC 2 cut(s) 1946, 2155
PspXI VCTCGAGB 2 cut(s) 1950, 2321
PstNI CAGNNNCTG 3 cut(s) 791, 1696, 2465
PsuI RGATCY 1 cut(s) 2224
PvuII CAGCTG 1 cut(s) 1618
RsaI GTAC 3 cut(s) 820, 1269, 1541
RsaNI GTAC 3 cut(s) 819, 1268, 1540
RseI CAYNNNNRTG 2 cut(s) 1869, 1872
SaqAI TTAA 8 cut(s) 693, 942, 1380, 1392, 1476, 1632, 1662, 2521
SatI GCNGC 7 cut(s) 26, 699, 1298, 1826, 1850, 2300, 2466
Sau96I GGNCC 2 cut(s) 1946, 2155
ScaI AGTACT 1 cut(s) 820
SchI GAGTC 7 cut(s) 90, 1047, 1267, 1352, 1454, 1962, 2313
SduI GDGCHC 4 cut(s) 777, 1167, 1911, 2376
SfaNI GCATC 7 cut(s) 217, 703, 826, 2073, 2100, 2311, 2452
SfcI CTRYAG 2 cut(s) 879, 1613
Sfr274I CTCGAG 2 cut(s) 1950, 2321
SfuI TTCGAA 1 cut(s) 893
SinI GGWCC 1 cut(s) 2155
SlaI CTCGAG 2 cut(s) 1950, 2321
SmiMI CAYNNNNRTG 2 cut(s) 1869, 1872
SmlI CTYRAG 7 cut(s) 806, 913, 1475, 1950, 2074, 2321, 2343
SmoI CTYRAG 7 cut(s) 806, 913, 1475, 1950, 2074, 2321, 2343
SpeI ACTAGT 1 cut(s) 1231
SphI GCATGC 1 cut(s) 1601
SseBI AGGCCT 1 cut(s) 758
SsiI CCGC 4 cut(s) 66, 1262, 1275, 2153
SspMI CTAG 3 cut(s) 1232, 1265, 2426
StuI AGGCCT 1 cut(s) 758
TaaI ACNGT 3 cut(s) 544, 1729, 1899
TaiI ACGT 1 cut(s) 58
TaqI TCGA 6 cut(s) 893, 1119, 1503, 1516, 1951, 2322
TaqII GACCGA 1 cut(s) 1738
TatI WGTACW 3 cut(s) 818, 1267, 1539
Tru1I TTAA 8 cut(s) 693, 942, 1380, 1392, 1476, 1632, 1662, 2521
Tru9I TTAA 8 cut(s) 693, 942, 1380, 1392, 1476, 1632, 1662, 2521
TscAI CASTG 4 cut(s) 796, 1488, 1826, 1891
TseFI GTSAC 1 cut(s) 787
TseI GCWGC 7 cut(s) 25, 698, 1297, 1825, 1849, 2299, 2465
Tsp45I GTSAC 1 cut(s) 787
TspGWI ACGGA 2 cut(s) 443, 1067
TspRI CASTG 4 cut(s) 796, 1488, 1826, 1891
Vha464I CTTAAG 1 cut(s) 1475
VpaK11BI GGWCC 1 cut(s) 2155
VspI ATTAAT 1 cut(s) 1662
XceI RCATGY 3 cut(s) 76, 1601, 2383
XhoI CTCGAG 2 cut(s) 1950, 2321
XmiI GTMKAC 2 cut(s) 575, 1448
XmnI GAANNNNTTC 2 cut(s) 1815, 2288
XspI CTAG 3 cut(s) 1232, 1265, 2426
ZrmI AGTACT 1 cut(s) 820
Zsp2I ATGCAT 1 cut(s) 1599
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.