AT1G70330

Equilibrative nucleotide transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0014468
Physical Location & Seq
Forward (+)
26502909 .. 26504619
1711 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G70330.1

Sequence Viewer

Length: 1353 bp
ATGACTCCCATTAGTCAACGAATTTCCCAAAAATCCCCAAAACTGAGAGAAACTAAACAAATTCAAATGACCACCACCGATAAATCCGCCGGAATCGTAACTGACTCGGAAGCCGGACCGGAGACTTCTCTTCTCCTCAATCCTCACGAAGGATCTACCAAAAAAGCTCCTTCTGATTCCTACCACTTCGCTTACATCATCTACTTCACCCTCGGCGTTGGCTTTCTTCTTCCATGGAACGCATTCATCACCGCCGTCGATTACTTCTCTTACCTCTATCCTTCCACGGCCGTCGATCGGATCTTCGCCGTTATCTACATGCTCGTTGCTCTTGTTTGTCTATTCGTTATCGTTGTCTTCTATGCACATAAGAGCCTCGCTAGCTTTAGAATCAATCTCGGTCTTTTACTCTTCGTAATCGCCTTGCTTGTGGTTCCTGTTTTGGATCTGGTTTATGTTAAAGGACAGGTCGGATTATACGCCGGATTCGATGTTACTTCCGCCGCCGTTGCCTTATCCGGTCTAGGTGATGCACTTATGCAAGGAGGTCTTATCGGTGTTGCCGGTGAAATGCCGGAGAGGTATATGCAAGCTGTTGTCGCCGGAACTGCTGGCTCCGGTGTTCTTGTGTCACTATTGAGAATTTTGACTAAAGCTGTGTATCCTCAAGATCCTGATGGTTTGAGAAAAAGTGCGAATCTATACTTTGCTGTGGGGATTGTTGTTATGGTGATTTGTGCTGTGTTCTACAACGTAGCGCATAAGCTTCCGGTGATCAAGTTCCATGAGGAGCGTAAGAATGAAGAACTAATCAGAGAGAAGAGCGAAGAGAAAGGTTCTTTAACCGGTTTAGCGTGGAGGACAACACTCTGGGATATCGTGACGAAAGTTAAATCTCATGGTTTCGGGATTGTTCTCTTATACATGGTGACATTGTCGATATTTCCTGGATACATTACTGAGGATGTTCATTCAGAGCTTCTTACAGATTGGTACCCTATTCTGCTTATTGCAGCGTACAATGTGTTTGACTTGGTTGGTAAGTGTCTGACTGCTGTATTCATGCTGGAGGATGAGAAAATCGCTGTTGGTGGTTCCATTGCTAGGCTCTTGTTTTACCCTCTCTTCTGGGGTTGCTTGCACGGTCCGATGTTTCTCAGGACTGAGATTCCTGTAACTATACTGACATGCTTATTGGGACTTACCAACGGGTACTTGACAAGCGTCTTGATGATTCTGGCTCCAAAATCGGTTCCACTGAGACACTCGGAGACTGCAGGAATCGTCACTGTGATGTTCTTGGTTGTTGGTTTGGCCTCTGGATCTGTCATTGCTTGGTTCTGGGTCATTTGA

Protein Analysis

450

Amino Acids

49.34

Weight (kDa)

6.76

Isoelectric Point (pI)

31.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nucleoside_tran PF01733 168 - 450 4.1e-99 Nucleoside transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014468)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G70330
fragaria_vesca FvH4_4g34590
malus_domestica MD13G1091100.v1.1 MD16G1091600.v1.1
prunus_persica Prupe.1G254800_v2.0.a1
pyrus_communis pycom13g07930 pycom16g07820
rosa_chinensis RchiOBHm_Chr4g0443961
rosa_laevigata RLG00000005873
rosa_multiflora Rmu_sc0011390.1_g000001
rosa_roxburghii Rroxscaffold_5G00384500
rosa_rugosa Rorug04G0348500
rosa_samantha Rh4AG409200 Rh4BG420300 Rh4CG434300 Rh4DG415300
rosa_wichuraiana Rw4G035150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 993
AccB1I GGYRCC 1 cut(s) 993
AciI CCGC 4 cut(s) 87, 252, 501, 504
AclWI GGATC 5 cut(s) 160, 308, 453, 665, 1330
AcoI YGGCCR 1 cut(s) 288
AcsI RAATTY 3 cut(s) 21, 60, 642
AfaI GTAC 3 cut(s) 995, 1019, 1214
AfiI CCNNNNNNNGG 3 cut(s) 149, 297, 1104
AgeI ACCGGT 1 cut(s) 845
AgsI TTSAA 1 cut(s) 65
AjnI CCWGG 1 cut(s) 946
AluBI AGCT 6 cut(s) 167, 384, 593, 656, 766, 979
AluI AGCT 6 cut(s) 167, 384, 593, 656, 766, 979
Alw26I GTCTC 3 cut(s) 116, 1255, 1265
AlwI GGATC 5 cut(s) 160, 308, 453, 665, 1330
AoxI GGCC 2 cut(s) 288, 1314
ApeKI GCWGC 1 cut(s) 1013
ApoI RAATTY 3 cut(s) 21, 60, 642
AsiGI ACCGGT 1 cut(s) 845
Asp700I GAANNNNTTC 1 cut(s) 242
Asp718I GGTACC 1 cut(s) 993
AspLEI GCGC 1 cut(s) 760
AspS9I GGNCC 2 cut(s) 116, 1145
AsuHPI GGTGA 7 cut(s) 199, 241, 539, 578, 742, 784, 940
AsuNHI GCTAGC 1 cut(s) 380
AvaII GGWCC 2 cut(s) 116, 1145
BanI GGYRCC 1 cut(s) 993
BbsI GAAGAC 1 cut(s) 349
BbvI GCAGC 1 cut(s) 1025
BccI CCATC 1 cut(s) 671
BceAI ACGGC 5 cut(s) 239, 275, 293, 303, 491
BciT130I CCWGG 1 cut(s) 948
BciVI GTATCC 2 cut(s) 672, 944
BclI TGATCA 1 cut(s) 774
BcoDI GTCTC 3 cut(s) 116, 1255, 1265
BfaI CTAG 3 cut(s) 381, 524, 1104
BfmI CTRYAG 1 cut(s) 1275
BfuI GTATCC 2 cut(s) 672, 944
BisI GCNGC 2 cut(s) 504, 1014
BlsI GCNGC 2 cut(s) 505, 1015
Bme1390I CCNGG 1 cut(s) 948
Bme18I GGWCC 2 cut(s) 116, 1145
BmgT120I GGNCC 2 cut(s) 116, 1145
BmiI GGNNCC 6 cut(s) 435, 616, 995, 1096, 1242, 1254
BmrFI CCNGG 1 cut(s) 948
BmsI GCATC 1 cut(s) 520
BmtI GCTAGC 1 cut(s) 384
BoxI GACNNNNGTC 1 cut(s) 1223
BpiI GAAGAC 1 cut(s) 349
BpmI CTGGAG 1 cut(s) 1088
BpuEI CTTGAG 1 cut(s) 651
BsaJI CCNNGG 3 cut(s) 211, 233, 285
BsaWI WCCGGW 5 cut(s) 118, 518, 617, 769, 845
Bsc4I CCNNNNNNNGG 3 cut(s) 149, 297, 1104
Bse118I RCCGGY 2 cut(s) 563, 845
Bse3DI GCAATG 2 cut(s) 1098, 1329
BseBI CCWGG 1 cut(s) 948
BseDI CCNNGG 3 cut(s) 211, 233, 285
BseGI GGATG 2 cut(s) 970, 1078
BseLI CCNNNNNNNGG 3 cut(s) 149, 297, 1104
BseMI GCAATG 2 cut(s) 1098, 1329
BseMII CTCAG 5 cut(s) 35, 951, 1155, 1171, 1250
BseRI GAGGAG 2 cut(s) 125, 803
BseX3I CGGCCG 1 cut(s) 288
BseXI GCAGC 1 cut(s) 1025
Bsh1285I CGRYCG 2 cut(s) 291, 298
BshFI GGCC 2 cut(s) 290, 1316
BshNI GGYRCC 1 cut(s) 993
BshTI ACCGGT 1 cut(s) 845
BsiEI CGRYCG 2 cut(s) 291, 298
BslFI GGGAC 1 cut(s) 1212
BslI CCNNNNNNNGG 3 cut(s) 149, 297, 1104
BsmAI GTCTC 3 cut(s) 116, 1255, 1265
BsmFI GGGAC 1 cut(s) 1212
BsmI GAATGC 1 cut(s) 242
BsnI GGCC 2 cut(s) 290, 1316
Bsp143I GATC 7 cut(s) 152, 295, 300, 445, 670, 774, 1322
Bsp19I CCATGG 1 cut(s) 233
BspACI CCGC 4 cut(s) 87, 252, 501, 504
BspANI GGCC 2 cut(s) 290, 1316
BspCNI CTCAG 5 cut(s) 36, 952, 1156, 1170, 1251
BspLI GGNNCC 6 cut(s) 435, 616, 995, 1096, 1242, 1254
BspMAI CTGCAG 1 cut(s) 1279
BspOI GCTAGC 1 cut(s) 384
BspPI GGATC 5 cut(s) 160, 308, 453, 665, 1330
BspQI GCTCTTC 1 cut(s) 815
BspT107I GGYRCC 1 cut(s) 993
BsrDI GCAATG 2 cut(s) 1098, 1329
BsrFI RCCGGY 2 cut(s) 563, 845
BssAI RCCGGY 2 cut(s) 563, 845
BssECI CCNNGG 3 cut(s) 211, 233, 285
BssMI GATC 7 cut(s) 152, 295, 300, 445, 670, 774, 1322
BssT1I CCWWGG 1 cut(s) 233
Bst2UI CCWGG 1 cut(s) 948
Bst4CI ACNGT 2 cut(s) 1145, 1291
Bst6I CTCTTC 5 cut(s) 135, 416, 815, 822, 1130
BstC8I GCNNGC 4 cut(s) 382, 591, 613, 1139
BstDEI CTNAG 5 cut(s) 44, 960, 1157, 1164, 1259
BstDSI CCRYGG 2 cut(s) 233, 285
BstENI CCTNNNNNAGG 1 cut(s) 147
BstF5I GGATG 2 cut(s) 970, 1078
BstHHI GCGC 1 cut(s) 760
BstKTI GATC 7 cut(s) 155, 298, 303, 448, 673, 777, 1325
BstMAI GTCTC 3 cut(s) 116, 1255, 1265
BstMBI GATC 7 cut(s) 152, 295, 300, 445, 670, 774, 1322
BstMCI CGRYCG 2 cut(s) 291, 298
BstMWI GCNNNNNNNGC 4 cut(s) 381, 509, 599, 608
BstNI CCWGG 1 cut(s) 948
BstNSI RCATGY 2 cut(s) 322, 1191
BstPAI GACNNNNGTC 1 cut(s) 1223
BstSCI CCNGG 1 cut(s) 946
BstSFI CTRYAG 1 cut(s) 1275
BstV1I GCAGC 1 cut(s) 1025
BstV2I GAAGAC 1 cut(s) 349
BstX2I RGATCY 5 cut(s) 152, 300, 445, 670, 1322
BstYI RGATCY 5 cut(s) 152, 300, 445, 670, 1322
BstZI CGGCCG 1 cut(s) 288
BsuI GTATCC 2 cut(s) 672, 944
BsuRI GGCC 2 cut(s) 290, 1316
BtgI CCRYGG 2 cut(s) 233, 285
BtsCI GGATG 2 cut(s) 970, 1078
BtsIMutI CAGTG 2 cut(s) 1256, 1287
Cac8I GCNNGC 4 cut(s) 382, 591, 613, 1139
CfoI GCGC 1 cut(s) 760
Cfr10I RCCGGY 2 cut(s) 563, 845
Cfr13I GGNCC 2 cut(s) 116, 1145
CpoI CGGWCCG 2 cut(s) 116, 1145
CseI GACGC 1 cut(s) 1213
Csp6I GTAC 3 cut(s) 994, 1018, 1213
CspAI ACCGGT 1 cut(s) 845
CspI CGGWCCG 2 cut(s) 116, 1145
CviAII CATG 7 cut(s) 234, 319, 785, 899, 925, 1063, 1188
CviQI GTAC 3 cut(s) 994, 1018, 1213
DdeI CTNAG 5 cut(s) 44, 960, 1157, 1164, 1259
DpnI GATC 7 cut(s) 154, 297, 302, 447, 672, 776, 1324
DpnII GATC 7 cut(s) 152, 295, 300, 445, 670, 774, 1322
EaeI YGGCCR 1 cut(s) 288
EagI CGGCCG 1 cut(s) 288
Eam1104I CTCTTC 5 cut(s) 135, 416, 815, 822, 1130
EarI CTCTTC 5 cut(s) 135, 416, 815, 822, 1130
EciI GGCGGA 2 cut(s) 76, 490
EclXI CGGCCG 1 cut(s) 288
Eco130I CCWWGG 1 cut(s) 233
Eco32I GATATC 1 cut(s) 877
Eco47I GGWCC 2 cut(s) 116, 1145
Eco52I CGGCCG 1 cut(s) 288
EcoNI CCTNNNNNAGG 1 cut(s) 147
EcoRII CCWGG 1 cut(s) 946
EcoRV GATATC 1 cut(s) 877
EcoT14I CCWWGG 1 cut(s) 233
ErhI CCWWGG 1 cut(s) 233
FaeI CATG 7 cut(s) 237, 322, 788, 902, 928, 1066, 1191
FalI AAGNNNNNCTT 2 cut(s) 534, 566
FaqI GGGAC 1 cut(s) 1212
FatI CATG 7 cut(s) 233, 318, 784, 898, 924, 1062, 1187
FbaI TGATCA 1 cut(s) 774
Fnu4HI GCNGC 2 cut(s) 504, 1014
FokI GGATG 2 cut(s) 977, 1085
Fsp4HI GCNGC 2 cut(s) 504, 1014
FspBI CTAG 3 cut(s) 381, 524, 1104
GlaI GCGC 1 cut(s) 759
GluI GCNGC 2 cut(s) 504, 1014
GsuI CTGGAG 1 cut(s) 1088
HaeIII GGCC 2 cut(s) 290, 1316
HgaI GACGC 1 cut(s) 1213
HhaI GCGC 1 cut(s) 760
Hin1II CATG 7 cut(s) 237, 322, 788, 902, 928, 1066, 1191
Hin6I GCGC 1 cut(s) 758
HinP1I GCGC 1 cut(s) 758
HincII GTYRAC 1 cut(s) 17
HindII GTYRAC 1 cut(s) 17
HindIII AAGCTT 1 cut(s) 764
HphI GGTGA 7 cut(s) 199, 241, 539, 578, 742, 784, 940
Hpy166II GTNNAC 1 cut(s) 17
Hpy188I TCNGA 9 cut(s) 109, 175, 300, 473, 815, 976, 1050, 1149, 1270
Hpy188III TCNNGA 8 cut(s) 146, 668, 674, 880, 907, 1159, 1228, 1320
Hpy8I GTNNAC 1 cut(s) 17
Hpy99I CGWCG 2 cut(s) 260, 296
HpyAV CCTTC 3 cut(s) 143, 180, 291
HpyCH4III ACNGT 2 cut(s) 1145, 1291
HpyCH4IV ACGT 1 cut(s) 753
HpyCH4V TGCA 7 cut(s) 365, 533, 541, 589, 1013, 1141, 1277
HpyF10VI GCNNNNNNNGC 4 cut(s) 381, 509, 599, 608
HpyF3I CTNAG 5 cut(s) 44, 960, 1157, 1164, 1259
HpySE526I ACGT 1 cut(s) 753
Hsp92II CATG 7 cut(s) 237, 322, 788, 902, 928, 1066, 1191
HspAI GCGC 1 cut(s) 758
KpnI GGTACC 1 cut(s) 997
Ksp22I TGATCA 1 cut(s) 774
Kzo9I GATC 7 cut(s) 152, 295, 300, 445, 670, 774, 1322
LguI GCTCTTC 1 cut(s) 815
LmnI GCTCC 4 cut(s) 172, 620, 790, 1246
Lsp1109I GCAGC 1 cut(s) 1025
LweI GCATC 1 cut(s) 520
MaeI CTAG 3 cut(s) 381, 524, 1104
MaeII ACGT 1 cut(s) 753
MaeIII GTNAC 7 cut(s) 97, 493, 630, 880, 928, 1174, 1285
MalI GATC 7 cut(s) 154, 297, 302, 447, 672, 776, 1324
MboI GATC 7 cut(s) 152, 295, 300, 445, 670, 774, 1322
MflI RGATCY 5 cut(s) 152, 300, 445, 670, 1322
MluCI AATT 3 cut(s) 21, 60, 642
MlyI GAGTC 1 cut(s) 98
MmeI TCCRAC 1 cut(s) 451
MroXI GAANNNNTTC 1 cut(s) 242
MseI TTAA 3 cut(s) 459, 842, 891
MslI CAYNNNNRTG 1 cut(s) 1292
MspR9I CCNGG 1 cut(s) 948
Mva1269I GAATGC 1 cut(s) 242
MvaI CCWGG 1 cut(s) 948
MwoI GCNNNNNNNGC 4 cut(s) 381, 509, 599, 608
NcoI CCATGG 1 cut(s) 233
NdeII GATC 7 cut(s) 152, 295, 300, 445, 670, 774, 1322
NheI GCTAGC 1 cut(s) 380
NlaIII CATG 7 cut(s) 237, 322, 788, 902, 928, 1066, 1191
NlaIV GGNNCC 6 cut(s) 435, 616, 995, 1096, 1242, 1254
NmeAIII GCCGAG 1 cut(s) 192
NmuCI GTSAC 4 cut(s) 630, 880, 928, 1285
NspI RCATGY 2 cut(s) 322, 1191
PciSI GCTCTTC 1 cut(s) 815
PcsI WCGNNNNNNNCGW 1 cut(s) 486
PctI GAATGC 1 cut(s) 242
PdmI GAANNNNTTC 1 cut(s) 242
PfeI GAWTC 8 cut(s) 93, 176, 390, 486, 697, 1168, 1234, 1281
PflFI GACNNNGTC 1 cut(s) 934
PfoI TCCNGGA 1 cut(s) 946
PinAI ACCGGT 1 cut(s) 845
PkrI GCNGC 2 cut(s) 505, 1015
Ple19I CGATCG 1 cut(s) 298
PleI GAGTC 1 cut(s) 98
PpsI GAGTC 1 cut(s) 98
PshAI GACNNNNGTC 1 cut(s) 1223
Psp6I CCWGG 1 cut(s) 946
PspGI CCWGG 1 cut(s) 946
PspN4I GGNNCC 6 cut(s) 435, 616, 995, 1096, 1242, 1254
PspPI GGNCC 2 cut(s) 116, 1145
PstI CTGCAG 1 cut(s) 1279
PsuI RGATCY 5 cut(s) 152, 300, 445, 670, 1322
PsyI GACNNNGTC 1 cut(s) 934
PvuI CGATCG 1 cut(s) 298
RsaI GTAC 3 cut(s) 995, 1019, 1214
RsaNI GTAC 3 cut(s) 994, 1018, 1213
RseI CAYNNNNRTG 1 cut(s) 1292
Rsr2I CGGWCCG 2 cut(s) 116, 1145
RsrII CGGWCCG 2 cut(s) 116, 1145
SapI GCTCTTC 1 cut(s) 815
SaqAI TTAA 3 cut(s) 459, 842, 891
SatI GCNGC 2 cut(s) 504, 1014
Sau3AI GATC 7 cut(s) 152, 295, 300, 445, 670, 774, 1322
Sau96I GGNCC 2 cut(s) 116, 1145
SchI GAGTC 1 cut(s) 98
ScrFI CCNGG 1 cut(s) 948
SfaNI GCATC 1 cut(s) 520
SfcI CTRYAG 1 cut(s) 1275
SinI GGWCC 2 cut(s) 116, 1145
SmiMI CAYNNNNRTG 1 cut(s) 1292
SmlI CTYRAG 1 cut(s) 666
SmoI CTYRAG 1 cut(s) 666
Sse9I AATT 3 cut(s) 21, 60, 642
SsiI CCGC 4 cut(s) 87, 252, 501, 504
SspMI CTAG 3 cut(s) 381, 524, 1104
StyD4I CCNGG 1 cut(s) 946
StyI CCWWGG 1 cut(s) 233
TaaI ACNGT 2 cut(s) 1145, 1291
TaiI ACGT 1 cut(s) 756
TaqI TCGA 4 cut(s) 258, 294, 489, 938
TaqII GACCGA 1 cut(s) 389
TasI AATT 3 cut(s) 21, 60, 642
TauI GCSGC 1 cut(s) 506
TfiI GAWTC 8 cut(s) 93, 176, 390, 486, 697, 1168, 1234, 1281
Tru1I TTAA 3 cut(s) 459, 842, 891
Tru9I TTAA 3 cut(s) 459, 842, 891
TscAI CASTG 2 cut(s) 1263, 1294
TseFI GTSAC 4 cut(s) 630, 880, 928, 1285
TseI GCWGC 1 cut(s) 1013
Tsp45I GTSAC 4 cut(s) 630, 880, 928, 1285
TspDTI ATGAA 4 cut(s) 235, 816, 959, 1051
TspRI CASTG 2 cut(s) 1263, 1294
Tth111I GACNNNGTC 1 cut(s) 934
VpaK11BI GGWCC 2 cut(s) 116, 1145
XagI CCTNNNNNAGG 1 cut(s) 147
XapI RAATTY 3 cut(s) 21, 60, 642
XceI RCATGY 2 cut(s) 322, 1191
XmnI GAANNNNTTC 1 cut(s) 242
XspI CTAG 3 cut(s) 381, 524, 1104
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.