pycom16g07820

Equilibrative nucleotide transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0014468
Physical Location & Seq
Forward (+)
5149774 .. 5151497
1724 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g07820.2

Sequence Viewer

Length: 1275 bp
ATGGGTTTCAATGTCAAAACCACCGATGAAAATTCCGAGACCAGCCTCCTCCTCCCCACCGTAAGCGCCGCCGCCGCCGTCGTACCTAATAAAATTCCAGAGGACTCGTTCCACTTCGCCTATATCATCTACTTCACACTGGGTCTCGGCTACCTCCTCCCATGGAACGCTTTCATCACAGCCGTCGATTACTTCGCCTACCTCTATCCAGATGCCAGCGTCGACCGTATATTCTCCGTCGTTTACATGGTGGTGGGCCTCTTCTGCCTCCTCCTGATTATCTTCTACTCCCACAAGTCCGAAGCCTATGTCCGGATCAATGTGGGCCTGGGCCTCTTCGTCGTTTCGCTGCTCGTCGTTCCCCTCATGGATGTCTTTTACATCAAGGGTCGGGTCGGGTTGTACGACGGGTTTTACGTGACGGTTGCGGCGGTTGCGCTTTCCTCCGTGGCGGATGCTTTGGTTCAAGGCTCTCTCATTGGGGCGGCTAGTGAGTTGCCCGGAACTTATATGCAAGCCGTTGTGGCCGGGACTGCTGGTTCAGGAGTCATTGTTTCTGCTCTGAGGATCGTAACCAAGGCTGTATATCCACAAGATACCGATGGCCTGAGGAAAAGTGCGAACCTTTACTTCGCTGTCGGGATAGTCATTATTGTCATATGCATTGTCTTCTACAATGTGGCACCAAGGATTCCAGTTATGAAGTATTATGCAGAGTTAAAGGTTCAGGCTGTAAACGATGCGACAGAAGAGAGAGGCCCTCCGACTCTGACTGTGTTGAGATCAACTCTGTGGCACATAGTCGACAGAGTCAAGTGGTACGGAATTAGCATCTTCCTCATCTATGTCGTGACTTTGTCGATATTTCCAGGATACATTACTGAAGACGTGCACTCTCAGATTCTCAAGGATTGGTACCCAATCATCCTTATCACCGGCTACAATGTGTTTGATCTCGTTGGCAAGTCTCTGACTTCTGTATGCCTCCTCAAAAATTCCAAGGTTGCAATCGGCAGTACAGTTGTGCGATTGCTCTTCTTTCCTCTCTTCTATGGGTGCTTGCACGGCCCCAAATTCTTCCGAACAGAGATTCCAGTGACGATACTAACCTGCCTTCTGGGGCTCACCAACGGCTACTTGACTAGCGTGTTGATGATTTTGGCTCCCAAAGTTGTCCAATTCCAACATGCTGAGACGGCAGGGATTGTGATCGTGTTGTTCTTGGTTCTGGGTTTGGCTGCTGGCGCAGTTGTATCTTGGTTCTGGGTCATCTGA

Protein Analysis

425

Amino Acids

46.49

Weight (kDa)

6.99

Isoelectric Point (pI)

27.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014468)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G70330
fragaria_vesca FvH4_4g34590
malus_domestica MD13G1091100.v1.1 MD16G1091600.v1.1
prunus_persica Prupe.1G254800_v2.0.a1
pyrus_communis pycom13g07930 pycom16g07820
rosa_chinensis RchiOBHm_Chr4g0443961
rosa_laevigata RLG00000005873
rosa_multiflora Rmu_sc0011390.1_g000001
rosa_roxburghii Rroxscaffold_5G00384500
rosa_rugosa Rorug04G0348500
rosa_samantha Rh4AG409200 Rh4BG420300 Rh4CG434300 Rh4DG415300
rosa_wichuraiana Rw4G035150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1118
Acc65I GGTACC 1 cut(s) 915
AccB1I GGYRCC 2 cut(s) 682, 915
AccI GTMKAC 2 cut(s) 222, 804
AccIII TCCGGA 1 cut(s) 312
AciI CCGC 7 cut(s) 69, 72, 75, 428, 431, 452, 485
AclWI GGATC 2 cut(s) 323, 575
AcoI YGGCCR 1 cut(s) 525
AcsI RAATTY 4 cut(s) 31, 93, 994, 1073
AcuI CTGAAG 1 cut(s) 903
AfaI GTAC 5 cut(s) 84, 404, 821, 917, 1018
AfiI CCNNNNNNNGG 1 cut(s) 312
AgsI TTSAA 2 cut(s) 10, 467
AjiI CACGTC 1 cut(s) 889
AjnI CCWGG 2 cut(s) 327, 868
AloI GAACNNNNNNTCC 4 cut(s) 523, 555, 1075, 1107
Alw21I GWGCWC 1 cut(s) 894
Alw26I GTCTC 4 cut(s) 32, 149, 972, 1187
Alw44I GTGCAC 1 cut(s) 890
AlwI GGATC 2 cut(s) 323, 575
Aor13HI TCCGGA 1 cut(s) 312
AoxI GGCC 7 cut(s) 256, 325, 331, 525, 604, 757, 1066
ApaLI GTGCAC 1 cut(s) 890
ApeKI GCWGC 2 cut(s) 349, 1238
ApoI RAATTY 4 cut(s) 31, 93, 994, 1073
ArsI GACNNNNNNTTYG 2 cut(s) 294, 326
Asp700I GAANNNNTTC 1 cut(s) 170
Asp718I GGTACC 1 cut(s) 915
AspLEI GCGC 3 cut(s) 68, 439, 1247
AspS9I GGNCC 5 cut(s) 256, 325, 331, 758, 1067
AsuC2I CCSGG 2 cut(s) 501, 529
AsuHPI GGTGA 2 cut(s) 925, 1117
AxyI CCTNAGG 1 cut(s) 608
BaeGI GKGCMC 1 cut(s) 894
BanI GGYRCC 2 cut(s) 682, 915
BanII GRGCYC 1 cut(s) 1125
BbsI GAAGAC 2 cut(s) 661, 891
Bbv12I GWGCWC 1 cut(s) 894
BbvI GCAGC 2 cut(s) 336, 1225
BccI CCATC 1 cut(s) 596
BceAI ACGGC 6 cut(s) 62, 167, 503, 1081, 1147, 1212
BciT130I CCWGG 2 cut(s) 329, 870
BciVI GTATCC 1 cut(s) 866
BcnI CCSGG 2 cut(s) 501, 529
BcoDI GTCTC 4 cut(s) 32, 149, 972, 1187
BfaI CTAG 2 cut(s) 489, 1143
BfoI RGCGCY 1 cut(s) 69
BfuAI ACCTGC 1 cut(s) 1118
BfuI GTATCC 1 cut(s) 866
BglI GCCNNNNNGGC 1 cut(s) 524
BisI GCNGC 7 cut(s) 69, 72, 75, 350, 429, 486, 1239
BlsI GCNGC 7 cut(s) 70, 73, 76, 351, 430, 487, 1240
Bme1390I CCNGG 4 cut(s) 329, 501, 529, 870
BmgBI CACGTC 1 cut(s) 889
BmgT120I GGNCC 5 cut(s) 256, 325, 331, 758, 1067
BmiI GGNNCC 4 cut(s) 684, 917, 1069, 1164
BmrFI CCNGG 4 cut(s) 329, 501, 529, 870
BmrI ACTGGG 1 cut(s) 149
BmsI GCATC 4 cut(s) 202, 445, 730, 840
BmuI ACTGGG 1 cut(s) 149
BpiI GAAGAC 2 cut(s) 661, 891
BplI GAGNNNNNCTC 4 cut(s) 745, 777, 772, 804
BpuEI CTTGAG 1 cut(s) 890
BpuMI CCSGG 2 cut(s) 501, 529
BsaAI YACGTR 1 cut(s) 418
BsaBI GATNNNNATC 1 cut(s) 1208
BsaI GGTCTC 2 cut(s) 32, 149
BsaJI CCNNGG 6 cut(s) 161, 328, 447, 576, 686, 999
BsaWI WCCGGW 1 cut(s) 312
BsaXI ACNNNNNCTCC 2 cut(s) 537, 567
Bsc4I CCNNNNNNNGG 1 cut(s) 312
Bse118I RCCGGY 1 cut(s) 935
Bse1I ACTGG 3 cut(s) 144, 695, 1094
Bse21I CCTNAGG 1 cut(s) 608
Bse8I GATNNNNATC 1 cut(s) 1208
BseAI TCCGGA 1 cut(s) 312
BseBI CCWGG 2 cut(s) 329, 870
BseDI CCNNGG 6 cut(s) 161, 328, 447, 576, 686, 999
BseGI GGATG 3 cut(s) 376, 460, 924
BseJI GATNNNNATC 1 cut(s) 1208
BseLI CCNNNNNNNGG 1 cut(s) 312
BseMII CTCAG 4 cut(s) 554, 599, 911, 1182
BseNI ACTGG 3 cut(s) 144, 695, 1094
BseRI GAGGAG 5 cut(s) 38, 41, 146, 260, 977
BseSI GKGCMC 1 cut(s) 894
BseXI GCAGC 2 cut(s) 336, 1225
Bsh1285I CGRYCG 1 cut(s) 226
BshFI GGCC 7 cut(s) 258, 327, 333, 527, 606, 759, 1068
BshNI GGYRCC 2 cut(s) 682, 915
BsiEI CGRYCG 1 cut(s) 226
BsiHKAI GWGCWC 1 cut(s) 894
BsiSI CCGG 4 cut(s) 313, 501, 528, 936
BslFI GGGAC 1 cut(s) 544
BslI CCNNNNNNNGG 1 cut(s) 312
BsmAI GTCTC 4 cut(s) 32, 149, 972, 1187
BsmBI CGTCTC 1 cut(s) 1187
BsmFI GGGAC 1 cut(s) 544
BsnI GGCC 7 cut(s) 258, 327, 333, 527, 606, 759, 1068
Bso31I GGTCTC 2 cut(s) 32, 149
Bsp1286I GDGCHC 2 cut(s) 894, 1125
Bsp13I TCCGGA 1 cut(s) 312
Bsp143I GATC 5 cut(s) 315, 567, 782, 952, 1209
Bsp19I CCATGG 1 cut(s) 161
BspACI CCGC 7 cut(s) 69, 72, 75, 428, 431, 452, 485
BspANI GGCC 7 cut(s) 258, 327, 333, 527, 606, 759, 1068
BspCNI CTCAG 4 cut(s) 555, 600, 910, 1183
BspEI TCCGGA 1 cut(s) 312
BspLI GGNNCC 4 cut(s) 684, 917, 1069, 1164
BspMI ACCTGC 1 cut(s) 1118
BspPI GGATC 2 cut(s) 323, 575
BspQI GCTCTTC 1 cut(s) 1040
BspT107I GGYRCC 2 cut(s) 682, 915
BspTNI GGTCTC 2 cut(s) 32, 149
BsrFI RCCGGY 1 cut(s) 935
BsrI ACTGG 3 cut(s) 144, 695, 1094
BssAI RCCGGY 1 cut(s) 935
BssECI CCNNGG 6 cut(s) 161, 328, 447, 576, 686, 999
BssMI GATC 5 cut(s) 315, 567, 782, 952, 1209
BssT1I CCWWGG 4 cut(s) 161, 576, 686, 999
Bst2UI CCWGG 2 cut(s) 329, 870
Bst4CI ACNGT 5 cut(s) 61, 227, 424, 775, 1021
Bst6I CTCTTC 5 cut(s) 266, 341, 744, 1040, 1052
BstBAI YACGTR 1 cut(s) 418
BstC8I GCNNGC 4 cut(s) 217, 516, 1061, 1243
BstDEI CTNAG 4 cut(s) 563, 608, 897, 1191
BstDSI CCRYGG 2 cut(s) 161, 447
BstF5I GGATG 3 cut(s) 376, 460, 924
BstH2I RGCGCY 1 cut(s) 69
BstHHI GCGC 3 cut(s) 68, 439, 1247
BstKTI GATC 5 cut(s) 318, 570, 785, 955, 1212
BstMAI GTCTC 4 cut(s) 32, 149, 972, 1187
BstMBI GATC 5 cut(s) 315, 567, 782, 952, 1209
BstMCI CGRYCG 1 cut(s) 226
BstMWI GCNNNNNNNGC 8 cut(s) 74, 264, 434, 524, 533, 1065, 1196, 1244
BstNI CCWGG 2 cut(s) 329, 870
BstNSI RCATGY 1 cut(s) 1190
BstSCI CCNGG 4 cut(s) 327, 499, 527, 868
BstSLI GKGCMC 1 cut(s) 894
BstV1I GCAGC 2 cut(s) 336, 1225
BstV2I GAAGAC 2 cut(s) 661, 891
Bsu36I CCTNAGG 1 cut(s) 608
BsuI GTATCC 1 cut(s) 866
BsuRI GGCC 7 cut(s) 258, 327, 333, 527, 606, 759, 1068
BtgI CCRYGG 2 cut(s) 161, 447
BtrI CACGTC 1 cut(s) 889
BtsCI GGATG 3 cut(s) 376, 460, 924
BtsIMutI CAGTG 2 cut(s) 137, 1101
BveI ACCTGC 1 cut(s) 1118
Cac8I GCNNGC 4 cut(s) 217, 516, 1061, 1243
CfoI GCGC 3 cut(s) 68, 439, 1247
Cfr10I RCCGGY 1 cut(s) 935
Cfr13I GGNCC 5 cut(s) 256, 325, 331, 758, 1067
CseI GACGC 1 cut(s) 208
Csp6I GTAC 5 cut(s) 83, 403, 820, 916, 1017
CviAII CATG 4 cut(s) 162, 247, 367, 1187
CviQI GTAC 5 cut(s) 83, 403, 820, 916, 1017
DdeI CTNAG 4 cut(s) 563, 608, 897, 1191
DpnI GATC 5 cut(s) 317, 569, 784, 954, 1211
DpnII GATC 5 cut(s) 315, 567, 782, 952, 1209
EaeI YGGCCR 1 cut(s) 525
Eam1104I CTCTTC 5 cut(s) 266, 341, 744, 1040, 1052
EarI CTCTTC 5 cut(s) 266, 341, 744, 1040, 1052
EciI GGCGGA 1 cut(s) 467
Eco130I CCWWGG 4 cut(s) 161, 576, 686, 999
Eco24I GRGCYC 1 cut(s) 1125
Eco31I GGTCTC 2 cut(s) 32, 149
Eco57I CTGAAG 1 cut(s) 903
Eco81I CCTNAGG 1 cut(s) 608
EcoO109I RGGNCCY 1 cut(s) 758
EcoRII CCWGG 2 cut(s) 327, 868
EcoT14I CCWWGG 4 cut(s) 161, 576, 686, 999
EcoT22I ATGCAT 1 cut(s) 665
EcoT38I GRGCYC 1 cut(s) 1125
ErhI CCWWGG 4 cut(s) 161, 576, 686, 999
Esp3I CGTCTC 1 cut(s) 1187
FaeI CATG 4 cut(s) 165, 250, 370, 1190
FaqI GGGAC 1 cut(s) 544
FatI CATG 4 cut(s) 161, 246, 366, 1186
FauNDI CATATG 1 cut(s) 659
FblI GTMKAC 2 cut(s) 222, 804
Fnu4HI GCNGC 7 cut(s) 69, 72, 75, 350, 429, 486, 1239
FokI GGATG 3 cut(s) 383, 467, 911
FriOI GRGCYC 1 cut(s) 1125
Fsp4HI GCNGC 7 cut(s) 69, 72, 75, 350, 429, 486, 1239
FspBI CTAG 2 cut(s) 489, 1143
GlaI GCGC 3 cut(s) 67, 438, 1246
GluI GCNGC 7 cut(s) 69, 72, 75, 350, 429, 486, 1239
HaeII RGCGCY 1 cut(s) 69
HaeIII GGCC 7 cut(s) 258, 327, 333, 527, 606, 759, 1068
HapII CCGG 4 cut(s) 313, 501, 528, 936
HgaI GACGC 1 cut(s) 208
HhaI GCGC 3 cut(s) 68, 439, 1247
Hin1II CATG 4 cut(s) 165, 250, 370, 1190
Hin6I GCGC 3 cut(s) 66, 437, 1245
HinP1I GCGC 3 cut(s) 66, 437, 1245
HincII GTYRAC 2 cut(s) 223, 805
HindII GTYRAC 2 cut(s) 223, 805
HinfI GANTC 7 cut(s) 104, 546, 691, 766, 810, 901, 1090
HpaII CCGG 4 cut(s) 313, 501, 528, 936
HphI GGTGA 2 cut(s) 925, 1117
Hpy166II GTNNAC 5 cut(s) 223, 244, 736, 805, 892
Hpy188I TCNGA 9 cut(s) 37, 301, 564, 765, 771, 900, 972, 1082, 1274
Hpy188III TCNNGA 7 cut(s) 98, 209, 274, 313, 543, 640, 850
Hpy8I GTNNAC 5 cut(s) 223, 244, 736, 805, 892
Hpy99I CGWCG 7 cut(s) 83, 188, 224, 242, 344, 359, 410
HpyAV CCTTC 1 cut(s) 1124
HpyCH4III ACNGT 5 cut(s) 61, 227, 424, 775, 1021
HpyCH4IV ACGT 2 cut(s) 417, 888
HpyCH4V TGCA 6 cut(s) 514, 663, 713, 892, 1007, 1063
HpyF10VI GCNNNNNNNGC 8 cut(s) 74, 264, 434, 524, 533, 1065, 1196, 1244
HpyF3I CTNAG 4 cut(s) 563, 608, 897, 1191
HpySE526I ACGT 2 cut(s) 417, 888
Hsp92II CATG 4 cut(s) 165, 250, 370, 1190
HspAI GCGC 3 cut(s) 66, 437, 1245
Kpn2I TCCGGA 1 cut(s) 312
KpnI GGTACC 1 cut(s) 919
Kzo9I GATC 5 cut(s) 315, 567, 782, 952, 1209
LguI GCTCTTC 1 cut(s) 1040
LmnI GCTCC 1 cut(s) 1168
Lsp1109I GCAGC 2 cut(s) 336, 1225
LweI GCATC 4 cut(s) 202, 445, 730, 840
MaeI CTAG 2 cut(s) 489, 1143
MaeII ACGT 2 cut(s) 417, 888
MaeIII GTNAC 4 cut(s) 418, 571, 850, 1096
MalI GATC 5 cut(s) 317, 569, 784, 954, 1211
MboI GATC 5 cut(s) 315, 567, 782, 952, 1209
MhlI GDGCHC 2 cut(s) 894, 1125
MluCI AATT 6 cut(s) 31, 93, 825, 994, 1073, 1178
MlyI GAGTC 4 cut(s) 98, 555, 760, 819
MmeI TCCRAC 2 cut(s) 788, 1207
Mph1103I ATGCAT 1 cut(s) 665
MroI TCCGGA 1 cut(s) 312
MroXI GAANNNNTTC 1 cut(s) 170
MseI TTAA 1 cut(s) 719
MslI CAYNNNNRTG 1 cut(s) 251
MspI CCGG 4 cut(s) 313, 501, 528, 936
MspR9I CCNGG 4 cut(s) 329, 501, 529, 870
MvaI CCWGG 2 cut(s) 329, 870
MwoI GCNNNNNNNGC 8 cut(s) 74, 264, 434, 524, 533, 1065, 1196, 1244
NciI CCSGG 2 cut(s) 501, 529
NcoI CCATGG 1 cut(s) 161
NdeI CATATG 1 cut(s) 659
NdeII GATC 5 cut(s) 315, 567, 782, 952, 1209
NlaIII CATG 4 cut(s) 165, 250, 370, 1190
NlaIV GGNNCC 4 cut(s) 684, 917, 1069, 1164
NmeAIII GCCGAG 1 cut(s) 126
NmuCI GTSAC 3 cut(s) 418, 850, 1096
NsiI ATGCAT 1 cut(s) 665
NspI RCATGY 1 cut(s) 1190
PciSI GCTCTTC 1 cut(s) 1040
PcsI WCGNNNNNNNCGW 2 cut(s) 402, 414
PdmI GAANNNNTTC 1 cut(s) 170
PfeI GAWTC 3 cut(s) 691, 901, 1090
PflFI GACNNNGTC 2 cut(s) 809, 856
PfoI TCCNGGA 1 cut(s) 868
PkrI GCNGC 7 cut(s) 70, 73, 76, 351, 430, 487, 1240
PleI GAGTC 4 cut(s) 98, 554, 760, 818
PpsI GAGTC 4 cut(s) 98, 554, 760, 818
Ppu21I YACGTR 1 cut(s) 418
Psp6I CCWGG 2 cut(s) 327, 868
PspGI CCWGG 2 cut(s) 327, 868
PspN4I GGNNCC 4 cut(s) 684, 917, 1069, 1164
PspPI GGNCC 5 cut(s) 256, 325, 331, 758, 1067
PsyI GACNNNGTC 2 cut(s) 809, 856
RsaI GTAC 5 cut(s) 84, 404, 821, 917, 1018
RsaNI GTAC 5 cut(s) 83, 403, 820, 916, 1017
RseI CAYNNNNRTG 1 cut(s) 251
SalI GTCGAC 2 cut(s) 221, 803
SapI GCTCTTC 1 cut(s) 1040
SaqAI TTAA 1 cut(s) 719
SatI GCNGC 7 cut(s) 69, 72, 75, 350, 429, 486, 1239
Sau3AI GATC 5 cut(s) 315, 567, 782, 952, 1209
Sau96I GGNCC 5 cut(s) 256, 325, 331, 758, 1067
SchI GAGTC 4 cut(s) 98, 555, 760, 819
ScrFI CCNGG 4 cut(s) 329, 501, 529, 870
SduI GDGCHC 2 cut(s) 894, 1125
SetI ASST 9 cut(s) 88, 156, 204, 420, 627, 726, 891, 1005, 1112
SfaNI GCATC 4 cut(s) 202, 445, 730, 840
SmiMI CAYNNNNRTG 1 cut(s) 251
SmlI CTYRAG 1 cut(s) 905
SmoI CTYRAG 1 cut(s) 905
Sse9I AATT 6 cut(s) 31, 93, 825, 994, 1073, 1178
SsiI CCGC 7 cut(s) 69, 72, 75, 428, 431, 452, 485
SspMI CTAG 2 cut(s) 489, 1143
StyD4I CCNGG 4 cut(s) 327, 499, 527, 868
StyI CCWWGG 4 cut(s) 161, 576, 686, 999
TaaI ACNGT 5 cut(s) 61, 227, 424, 775, 1021
TaiI ACGT 2 cut(s) 420, 891
TaqI TCGA 4 cut(s) 186, 222, 804, 860
TasI AATT 6 cut(s) 31, 93, 825, 994, 1073, 1178
TatI WGTACW 1 cut(s) 1016
TauI GCSGC 5 cut(s) 71, 74, 77, 431, 488
TfiI GAWTC 3 cut(s) 691, 901, 1090
Tru1I TTAA 1 cut(s) 719
Tru9I TTAA 1 cut(s) 719
TscAI CASTG 2 cut(s) 144, 1101
TseFI GTSAC 3 cut(s) 418, 850, 1096
TseI GCWGC 2 cut(s) 349, 1238
Tsp45I GTSAC 3 cut(s) 418, 850, 1096
TspDTI ATGAA 3 cut(s) 42, 163, 716
TspGWI ACGGA 3 cut(s) 226, 436, 837
TspRI CASTG 2 cut(s) 144, 1101
Tth111I GACNNNGTC 2 cut(s) 809, 856
VneI GTGCAC 1 cut(s) 890
XapI RAATTY 4 cut(s) 31, 93, 994, 1073
XceI RCATGY 1 cut(s) 1190
XmiI GTMKAC 2 cut(s) 222, 804
XmnI GAANNNNTTC 1 cut(s) 170
XspI CTAG 2 cut(s) 489, 1143
Zsp2I ATGCAT 1 cut(s) 665
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.