Rorug04G0348500

Equilibrative nucleotide transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0004755
Physical Location & Seq
Forward (+)
52073757 .. 52086968
13212 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0348500.1

Sequence Viewer

Length: 4221 bp
ATGAGCAACTCGGTGAGCCATAATCTTCTCCAGCAGAATCTGCTTCAGAGCAAAATCAATTCTTCAGGCATTCCTGCCAACACATTGTTACAGCCCAAGTGCATACATCCAGTGCCAGCGCAGCCGCGCAAGTCTTCTTCTATATCCAACAAATTTTGTGGCAACAGTTTGCAGAAGCCGAAGCTGGCCATGGGGTCTCGTCGGCCTGTTACATTTGTTCCTTGCGCTGTACTAACCACGGATCCGCAGTCTGATCTTGCCGGGAAATACAACCTTGATGAGAACATTGAATTGCAGGTTTATGTTGATGCTTCCTCTCCAGGGTCTGCCACTAGAGTAGATATTCAGGTCTCATATGACGGTGGTTCCTTGGTTCTTCATTGGGGTGGGATAAAAGATAGAAAAGAAAAATGGGTGCTTCCTTCTTGTCGCCCAGATGGAACCAAAGAATATAAGAACAGAGCTCTTAGAACTCCTTTTGCGAAATCTGGCTCCAATTCCGTACTTAAAATTGAGATTGATGATCCTGCAATACAAGCTATAGAGTTTCTCATAGTTGATGAAAGTCAGAACAGATGGTTTAAGAACAACGGCGGCAACTTTCATGTTAAATTACCTTTGAAAGAGAAGTTGATATCAAATGTTTCAGTTCCTGAAGATTTAGTGCAAATTCAAGCATATCTGAGGTGGGAGCGAAAGGGTAAACAGATGTACACACCAGAACAAGAGAAGGTGGAGTATGAAGCAGCCCGAACTGAGCTACTGGAGGAAGTAGCCAGGGGTGCTTCCATACAAGAGCTCCAAGCAAGGCTGACTAAGAAAACTGATTCTGGTAACAGTCAGGAACCATCTCATGCTGGTAACAAAGAAGTTTCCAAGGCCGCAAAGAAACATGAGAGTAAACAGGTTTTCAGAACTGGAGGAATTAAGCGGAAGCAACGAGACTTAATGAAGATTCTCAACAAACATGCTACTAAACCTGTAGATGAAGCTAAACTTGCACATGAAGAATCAACGAAACCAAAAGCCTTGAAAGCAGTTGAGCTATTTGCCAAGGCAAAAGAAGAACAAGATGGTGCTTCCGTCCTGAACAAAAAAATCTACAGGCTCGGTGATAAGGACCTGCTGGTACTTGTGACAAAGGTTGCTGACAAGATAAAGGTTCATCTGGCGACAGATATCAAAGAGCCAATTAACCTTCATTGGGCTCTATCTGTAAACAAAGCTGGAGAGTGGTTGGAACCACCTCCAAATGTACTGCCCCAGGGTTCAGTTTCTCTAAATGGGGCTGTTGAAACACAATTTGTATCTAGTTCTGTTGATTCTAATTATGAGGTTCAATCTTTAGAAATAGAAATGCAAGAGGAAAGTTTCAAAGGAATGCCATTTGTCCTTTGTTCTTCTGGAAACTGGATTAAGAACCAAGGCTCAGATTTCTATGCTGACTTTGGTGTTGCGGTCAAGAAAGTTCAGAAGGATGCTGGTGACGGGAAAGGTACTGCAAAGGATTTATTGGATAAAATAGCAGATCTGGAGAGTGAGGCGCAAAAATCCTTTATGCATCGGTTTAACATTGCAGCAGACTTGATAAATCAAGCAAAAGATGCTGGCGAATTAGGTCTTGCAGGTATTCTGGTGTGGATGAGGTTTATGGCTACGAGGCAGCTGATTTGGAATAAAAATTACAATGTCAAACCACGTGAGATTAGTAAAGCACAGGACAGGCTCACAGGCTTGCTCCAGAGTATTTACACAAGTCATCCACAGTATCGAGAGCTTTTACGGATGATATTGTCTACTGTTGGACGTGGAGGAGAAGGGGATGTGGGACAGCGAATTCGAGATGAGATCCTTGTTATTCAGAGAAACTGTGAGTGTAAGGGCGGAATGATGGAGGAATGGCATCAAAAGTTGCATAATAACACTAGCCCAGATGATGTTGTTATATGTCAGGCGCTAATTGATTACATCAAAAGTGACTTTGATATTGGTGTTTACTGGAAAACTTTGAACAACAATGGAATTACAAAAGAACGTCTTCTAAGCTATGACCGTGCAATCCACAATGAACCAAGTTTCAGGAGAGATCAGAAAGAGCGTCTTCTGCATGATCTGGGGAACTACATGAGAACTTTAAAGGCAGTTCACTCAGGTGCAGATCTTGAGTCTGCCATCACAAATTGTTTGGGTTACAGTGCTCAGGGTCAAGGCTTCATGGTTGGAGTACAAATAAATCCTGTATCTGGCCTGCCCTCTGAAGTTCCAGGACTGCTGCAATTTGTAATGGAACATGTTGAGGACAAGAATGTGGAAGTCCTTATTGAGGGTTTGCTTGAGGCTCGTCAGGAGCTGTGGCCATTGCTCTCTAAGCCTAATGATCGCCTGAAGGATCTCTTATTTTTGGACATTGCCCTTGATTCTACTGTGCGAACCGCCATTGAGAGGGGATATGAAGAACTGAATAATGCTGGGCCTGAGAAAATTATGTACTTCATCTCAATGGTTCTAGAAAACCTGGCATTATCGTCAGATGATAATGAGGATCTCATATACTGTTTGAAGGGATGGAATCATGCATTAAACATGCTAAAGAGTAATACCGATAACTGGGCATTATATGCAAAATCAATCCTAGACAGAACCCGCCTGGCACTTGCAAACAAGGCAGAGTCGTATCACAGTATTTTACAGCCATCAGCAGAGTACCTTGGATCAAAGCTTGGAGTGGACCAAGGGGCTTTGAGCATATTTACTGAAGAAGTTATCCGTGCTGGATCAGCTGCCTCTTTGTCCGCACTCGTTAATCGACTTGATCCAGTTCTTCGGAAGACTGCTAATTTGGGAAGTTGGCAGGTTATCAGCCCAGTTGAAGTGGTTGGATATGTTGTAGTTGTGGACGAGTTGCTCACTGTCCAGAATAAAGTATACGATAAGCCGACAATTTTGGTGGCAAGAAGTGTAAGAGGTGAGGAAGAAATTCCAGATGGCACAGTTGCTGTGCTAACACCTGATATGCCAGACGTCCTATCTCATGTTTCTGTCCGAGCAAGAAATAGCAAGGTCTGTTTTGCGACGTGTTTTGACTCCAATATTCTAGCTGACCTCCAAGCTTGTGAAGGGAAGTTATTGCGTGTAAAGCCAACTCCTGCTGATGTAGTTTATAGCGAGGTTAATGAGAGTGAGCTAGGAGATGCAAGTTCAACTAATACAAATGAAGACATCCCAGCTCTGACATTAGTGAAGAAACAGTTTACGGGTAGATATGCCATATCATCTGATGAGTTCACAAGTGAAATGGTTGGAGCTAAATCACGTAATATTTCTTATATAAAAGGAAAACTGCCCTCTTGGGTTGGAATTCCTACATCAGTTGCCCTACCATTTGGAGTATTTGAAAAAGTTCTTTCTGAAGATTCAAATAAGGCTGTTGCTGACAAGTTAGAAATTCTAAAGAAAAAGTTAAAAGAAGGAGATTTTGGTTCACTTGGGGAGATTCGAGAGACAGTTTTACAGTTGACAGCACCACCCCCGTTGGTGCAAGAGCTGAAGACTAAGATGCAAAGTTCAGGAATGCCTTGGCCTGGTGATGAGGGTGAACAACGATGGGAACAAGCATGGTTGGCTATAAAAAAGGTCTGGGCTTCAAAGTGGAATGAGAGAGCATACTTCAGCACAAGAAAAGTGAAATTAGACCATGATTATCTTTGCATGGCTGTCCTTGTTCAAGAAATAATAAGTGCCGACTATGCATTCGTTATTCACACAACTAACCCATCTTCAGGAGACTCGTCGGAGATATATGCCGAGGTTGTCAAAGGTCTTGGAGAAACCTTAGTTGGAGCTTATCCTGGACGTGCCTTAAGTTTTATTAGCAAGAAAAATGCTCTGGACTCTCCTCAGGTGTTAGGTTACCCTAGCAAACCGGTTGGCCTCTTTATCAGACGGTCCATAATCTTCCGATCCGATTCCAATGGTGAAGATCTTGAAGGTTATGCTGGTGCAGGCCTTTATGACAGTGTGCCTATGGATGCGGAAGAGGAAGTCGTGCTTGACTACTCATCTGACCCACTCGTTACCGATGGGAACTTCCAAAAGACAATTCTGTCCAGCATTGCTCGCGCAGGAAGTGCAATCGAGGAGTTATATGGTACAGCACAAGACATTGAAGGTGTGATCAGAGATGGGAAACTCTATGTTGTCCAGACAAGACCCCAGATGTGA

Protein Analysis

1406

Amino Acids

155.92

Weight (kDa)

5.95

Isoelectric Point (pI)

42.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ig_N_CWD1 PF23166 88 - 205 6.6e-41 Alpha-glucan water dikinase 1 Ig-like N-terminal domain
DUF7067 PF23229 219 - 274 4.1e-28 Alpha-glucan water dikinase, DUF7067
Ig_N_CWD1 PF23166 364 - 482 3e-27 Alpha-glucan water dikinase 1 Ig-like N-terminal domain
GWD1_pHisD PF22973 949 - 1061 2.7e-53 Alpha-glucan water dikinase, phosphohistidine-like domain
PPDK_N PF01326 1204 - 1405 2.5e-19 Pyruvate phosphate dikinase, AMP/ATP-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 4102
AatII GACGTC 1 cut(s) 3024
Acc36I ACCTGC 4 cut(s) 286, 1131, 1616, 2842
AccI GTMKAC 2 cut(s) 1796, 2925
AccII CGCG 2 cut(s) 127, 4119
AcoI YGGCCR 2 cut(s) 186, 2354
AcsI RAATTY 6 cut(s) 152, 669, 1836, 2976, 3357, 3444
AcvI CACGTG 1 cut(s) 1700
AcyI GRCGYC 1 cut(s) 3021
AfiI CCNNNNNNNGG 8 cut(s) 321, 1204, 2243, 2323, 2442, 2607, 3581, 3779
AflII CTTAAG 1 cut(s) 3859
AflIII ACRYGT 2 cut(s) 2290, 3074
AgeI ACCGGT 1 cut(s) 3922
AjiI CACGTC 3 cut(s) 1808, 3075, 3854
AjnI CCWGG 8 cut(s) 319, 776, 1263, 2263, 2514, 2646, 3580, 3847
AjuI GAANNNNNNNTTGG 6 cut(s) 3459, 3491, 3819, 3851, 4083, 4115
AleI CACNNNNGTG 1 cut(s) 2151
Alw21I GWGCWC 3 cut(s) 466, 801, 2200
Alw26I GTCTC 5 cut(s) 201, 355, 936, 3494, 3777
AlwNI CAGNNNCTG 5 cut(s) 40, 326, 653, 2350, 2996
AoxI GGCC 9 cut(s) 186, 203, 879, 2245, 2354, 2471, 3578, 3928, 4003
ApeKI GCWGC 6 cut(s) 121, 746, 1577, 1663, 2272, 2780
ApoI RAATTY 6 cut(s) 152, 669, 1836, 2976, 3357, 3444
ArsI GACNNNNNNTTYG 6 cut(s) 1821, 1853, 2821, 2853, 3734, 3766
AsiGI ACCGGT 1 cut(s) 3922
Asp700I GAANNNNTTC 3 cut(s) 2037, 2976, 3399
AspLEI GCGC 6 cut(s) 121, 129, 227, 1546, 1957, 4121
AspS9I GGNCC 4 cut(s) 1120, 2471, 2728, 3945
AsuC2I CCSGG 1 cut(s) 262
AsuHPI GGTGA 7 cut(s) 25, 1124, 1496, 2978, 3596, 3605, 3986
AvaII GGWCC 3 cut(s) 1120, 2728, 3945
AxyI CCTNAGG 1 cut(s) 3897
BalI TGGCCA 2 cut(s) 188, 2356
BamHI GGATCC 1 cut(s) 241
BanII GRGCYC 3 cut(s) 466, 801, 1210
BbrPI CACGTG 1 cut(s) 1700
BbsI GAAGAC 6 cut(s) 126, 2030, 2093, 2834, 3222, 3554
Bbv12I GWGCWC 3 cut(s) 466, 801, 2200
BbvI GCAGC 6 cut(s) 133, 758, 1589, 1675, 2259, 2767
BceAI ACGGC 1 cut(s) 607
BciT130I CCWGG 8 cut(s) 321, 778, 1265, 2265, 2516, 2648, 3582, 3849
BclI TGATCA 1 cut(s) 4173
BcnI CCSGG 1 cut(s) 262
BcoDI GTCTC 5 cut(s) 201, 355, 936, 3494, 3777
BfaI CTAG 8 cut(s) 333, 1311, 1926, 2507, 2633, 3095, 3185, 3915
BfmI CTRYAG 3 cut(s) 540, 981, 1102
BfoI RGCGCY 1 cut(s) 1958
BfrI CTTAAG 1 cut(s) 3859
BfuAI ACCTGC 4 cut(s) 286, 1131, 1616, 2842
BglII AGATCT 3 cut(s) 1528, 2158, 3979
BisI GCNGC 9 cut(s) 122, 125, 595, 747, 882, 1578, 1664, 2273, 2781
BlsI GCNGC 9 cut(s) 123, 126, 596, 748, 883, 1579, 1665, 2274, 2782
Bme1390I CCNGG 9 cut(s) 262, 321, 778, 1265, 2265, 2516, 2648, 3582, 3849
Bme18I GGWCC 3 cut(s) 1120, 2728, 3945
BmgBI CACGTC 3 cut(s) 1808, 3075, 3854
BmgT120I GGNCC 4 cut(s) 1120, 2471, 2728, 3945
BmiI GGNNCC 6 cut(s) 243, 367, 442, 493, 846, 1242
BmrFI CCNGG 9 cut(s) 262, 321, 778, 1265, 2265, 2516, 2648, 3582, 3849
BmrI ACTGGG 2 cut(s) 2617, 2858
BmsI GCATC 8 cut(s) 298, 1468, 1570, 1594, 1912, 3181, 3546, 4018
BmuI ACTGGG 2 cut(s) 2617, 2858
BpiI GAAGAC 6 cut(s) 126, 2030, 2093, 2834, 3222, 3554
BpmI CTGGAG 7 cut(s) 14, 303, 785, 939, 1248, 1553, 1724
Bpu10I CCTNAGC 1 cut(s) 2199
BpuEI CTTGAG 2 cut(s) 2183, 2354
BpuMI CCSGG 1 cut(s) 262
BsaAI YACGTR 2 cut(s) 1700, 3314
BsaHI GRCGYC 1 cut(s) 3021
BsaI GGTCTC 2 cut(s) 201, 355
BsaWI WCCGGW 1 cut(s) 3922
BsaXI ACNNNNNCTCC 2 cut(s) 783, 813
Bsc4I CCNNNNNNNGG 8 cut(s) 321, 1204, 2243, 2323, 2442, 2607, 3581, 3779
Bse118I RCCGGY 1 cut(s) 3922
Bse1I ACTGG 8 cut(s) 110, 768, 922, 1415, 2003, 2612, 2816, 2864
Bse21I CCTNAGG 1 cut(s) 3897
Bse3DI GCAATG 4 cut(s) 1572, 2357, 2406, 4110
BseBI CCWGG 8 cut(s) 321, 778, 1265, 2265, 2516, 2648, 3582, 3849
BseGI GGATG 9 cut(s) 106, 1483, 1647, 1759, 1791, 1828, 2570, 3219, 4033
BseLI CCNNNNNNNGG 8 cut(s) 321, 1204, 2243, 2323, 2442, 2607, 3581, 3779
BseMI GCAATG 4 cut(s) 1572, 2357, 2406, 4110
BseMII CTCAG 7 cut(s) 674, 747, 1443, 2163, 2213, 2466, 3911
BseNI ACTGG 8 cut(s) 110, 768, 922, 1415, 2003, 2612, 2816, 2864
BseRI GAGGAG 3 cut(s) 1827, 3885, 4151
BseXI GCAGC 6 cut(s) 133, 758, 1589, 1675, 2259, 2767
BseYI CCCAGC 2 cut(s) 2468, 3223
BsgI GTGCAG 2 cut(s) 2175, 4020
Bsh1236I CGCG 2 cut(s) 127, 4119
BshFI GGCC 9 cut(s) 188, 205, 881, 2247, 2356, 2473, 3580, 3930, 4005
BshTI ACCGGT 1 cut(s) 3922
BsiHKAI GWGCWC 3 cut(s) 466, 801, 2200
BsiSI CCGG 2 cut(s) 261, 3923
BslFI GGGAC 1 cut(s) 1842
BslI CCNNNNNNNGG 8 cut(s) 321, 1204, 2243, 2323, 2442, 2607, 3581, 3779
BsmAI GTCTC 5 cut(s) 201, 355, 936, 3494, 3777
BsmFI GGGAC 1 cut(s) 1842
BsmI GAATGC 4 cut(s) 69, 1386, 3576, 3749
BsnI GGCC 9 cut(s) 188, 205, 881, 2247, 2356, 2473, 3580, 3930, 4005
Bso31I GGTCTC 2 cut(s) 201, 355
Bsp1286I GDGCHC 4 cut(s) 466, 801, 1210, 2200
Bsp1407I TGTACA 1 cut(s) 711
Bsp19I CCATGG 1 cut(s) 189
BspANI GGCC 9 cut(s) 188, 205, 881, 2247, 2356, 2473, 3580, 3930, 4005
BspCNI CTCAG 7 cut(s) 675, 748, 1442, 2162, 2212, 2467, 3910
BspFNI CGCG 2 cut(s) 127, 4119
BspLI GGNNCC 6 cut(s) 243, 367, 442, 493, 846, 1242
BspMI ACCTGC 4 cut(s) 286, 1131, 1616, 2842
BspTI CTTAAG 1 cut(s) 3859
BspTNI GGTCTC 2 cut(s) 201, 355
BsrDI GCAATG 4 cut(s) 1572, 2357, 2406, 4110
BsrFI RCCGGY 1 cut(s) 3922
BsrGI TGTACA 1 cut(s) 711
BsrI ACTGG 8 cut(s) 110, 768, 922, 1415, 2003, 2612, 2816, 2864
BssAI RCCGGY 1 cut(s) 3922
BssNAI GTATAC 1 cut(s) 2926
BssNI GRCGYC 1 cut(s) 3021
BssT1I CCWWGG 8 cut(s) 189, 369, 876, 1053, 1423, 2707, 2731, 3575
Bst1107I GTATAC 1 cut(s) 2926
Bst2UI CCWGG 8 cut(s) 321, 778, 1265, 2265, 2516, 2648, 3582, 3849
Bst6I CTCTTC 1 cut(s) 4029
BstACI GRCGYC 1 cut(s) 3021
BstAFI CTTAAG 1 cut(s) 3859
BstAPI GCANNNNNTGC 4 cut(s) 40, 1604, 2618, 4127
BstAUI TGTACA 1 cut(s) 711
BstBAI YACGTR 2 cut(s) 1700, 3314
BstC8I GCNNGC 7 cut(s) 117, 186, 1609, 1736, 2249, 4003, 4117
BstDSI CCRYGG 2 cut(s) 189, 237
BstEII GGTNACC 1 cut(s) 3908
BstENI CCTNNNNNAGG 1 cut(s) 2321
BstF5I GGATG 9 cut(s) 106, 1483, 1647, 1759, 1791, 1828, 2570, 3219, 4033
BstFNI CGCG 2 cut(s) 127, 4119
BstH2I RGCGCY 1 cut(s) 1958
BstHHI GCGC 6 cut(s) 121, 129, 227, 1546, 1957, 4121
BstMAI GTCTC 5 cut(s) 201, 355, 936, 3494, 3777
BstNI CCWGG 8 cut(s) 321, 778, 1265, 2265, 2516, 2648, 3582, 3849
BstNSI RCATGY 3 cut(s) 971, 2294, 2587
BstPI GGTNACC 1 cut(s) 3908
BstSCI CCNGG 9 cut(s) 260, 319, 776, 1263, 2263, 2514, 2646, 3580, 3847
BstSFI CTRYAG 3 cut(s) 540, 981, 1102
BstUI CGCG 2 cut(s) 127, 4119
BstV1I GCAGC 6 cut(s) 133, 758, 1589, 1675, 2259, 2767
BstV2I GAAGAC 6 cut(s) 126, 2030, 2093, 2834, 3222, 3554
BstX2I RGATCY 7 cut(s) 241, 1528, 1848, 2158, 2389, 2542, 3979
BstYI RGATCY 7 cut(s) 241, 1528, 1848, 2158, 2389, 2542, 3979
BstZ17I GTATAC 1 cut(s) 2926
Bsu36I CCTNAGG 1 cut(s) 3897
BsuRI GGCC 9 cut(s) 188, 205, 881, 2247, 2356, 2473, 3580, 3930, 4005
BtgI CCRYGG 2 cut(s) 189, 237
BtrI CACGTC 3 cut(s) 1808, 3075, 3854
BtsCI GGATG 9 cut(s) 106, 1483, 1647, 1759, 1791, 1828, 2570, 3219, 4033
BtsIMutI CAGTG 4 cut(s) 117, 2200, 2907, 4021
BveI ACCTGC 4 cut(s) 286, 1131, 1616, 2842
Cac8I GCNNGC 7 cut(s) 117, 186, 1609, 1736, 2249, 4003, 4117
CaiI CAGNNNCTG 5 cut(s) 40, 326, 653, 2350, 2996
CfoI GCGC 6 cut(s) 121, 129, 227, 1546, 1957, 4121
Cfr10I RCCGGY 1 cut(s) 3922
Cfr13I GGNCC 4 cut(s) 1120, 2471, 2728, 3945
CseI GACGC 1 cut(s) 2087
CspAI ACCGGT 1 cut(s) 3922
CspCI CAANNNNNGTGG 6 cut(s) 139, 174, 2928, 2963, 3513, 3548
DraI TTTAAA 1 cut(s) 2136
DrdI GACNNNNNNGTC 1 cut(s) 4102
DseDI GACNNNNNNGTC 1 cut(s) 4102
EaeI YGGCCR 2 cut(s) 186, 2354
Eam1104I CTCTTC 1 cut(s) 4029
EarI CTCTTC 1 cut(s) 4029
EciI GGCGGA 1 cut(s) 1899
Ecl136II GAGCTC 2 cut(s) 464, 799
Eco130I CCWWGG 8 cut(s) 189, 369, 876, 1053, 1423, 2707, 2731, 3575
Eco147I AGGCCT 1 cut(s) 4005
Eco24I GRGCYC 3 cut(s) 466, 801, 1210
Eco31I GGTCTC 2 cut(s) 201, 355
Eco32I GATATC 2 cut(s) 636, 1180
Eco47I GGWCC 3 cut(s) 1120, 2728, 3945
Eco53kI GAGCTC 2 cut(s) 464, 799
Eco72I CACGTG 1 cut(s) 1700
Eco81I CCTNAGG 1 cut(s) 3897
Eco91I GGTNACC 1 cut(s) 3908
EcoICRI GAGCTC 2 cut(s) 464, 799
EcoNI CCTNNNNNAGG 1 cut(s) 2321
EcoO109I RGGNCCY 1 cut(s) 1120
EcoO65I GGTNACC 1 cut(s) 3908
EcoRI GAATTC 2 cut(s) 1836, 3357
EcoRII CCWGG 8 cut(s) 319, 776, 1263, 2263, 2514, 2646, 3580, 3847
EcoRV GATATC 2 cut(s) 636, 1180
EcoT14I CCWWGG 8 cut(s) 189, 369, 876, 1053, 1423, 2707, 2731, 3575
EcoT22I ATGCAT 3 cut(s) 1563, 2578, 3751
EcoT38I GRGCYC 3 cut(s) 466, 801, 1210
ErhI CCWWGG 8 cut(s) 189, 369, 876, 1053, 1423, 2707, 2731, 3575
FaqI GGGAC 1 cut(s) 1842
FauI CCCGC 1 cut(s) 2651
FauNDI CATATG 1 cut(s) 355
FbaI TGATCA 1 cut(s) 4173
FblI GTMKAC 2 cut(s) 1796, 2925
Fnu4HI GCNGC 9 cut(s) 122, 125, 595, 747, 882, 1578, 1664, 2273, 2781
FokI GGATG 9 cut(s) 93, 1490, 1654, 1746, 1798, 1835, 2577, 3206, 4040
FriOI GRGCYC 3 cut(s) 466, 801, 1210
Fsp4HI GCNGC 9 cut(s) 122, 125, 595, 747, 882, 1578, 1664, 2273, 2781
FspBI CTAG 8 cut(s) 333, 1311, 1926, 2507, 2633, 3095, 3185, 3915
GlaI GCGC 6 cut(s) 120, 128, 226, 1545, 1956, 4120
GluI GCNGC 9 cut(s) 122, 125, 595, 747, 882, 1578, 1664, 2273, 2781
GsaI CCCAGC 2 cut(s) 2472, 3227
GsuI CTGGAG 7 cut(s) 14, 303, 785, 939, 1248, 1553, 1724
HaeII RGCGCY 1 cut(s) 1958
HaeIII GGCC 9 cut(s) 188, 205, 881, 2247, 2356, 2473, 3580, 3930, 4005
HapII CCGG 2 cut(s) 261, 3923
HgaI GACGC 1 cut(s) 2087
HhaI GCGC 6 cut(s) 121, 129, 227, 1546, 1957, 4121
Hin1I GRCGYC 1 cut(s) 3021
Hin6I GCGC 6 cut(s) 119, 127, 225, 1544, 1955, 4119
HinP1I GCGC 6 cut(s) 119, 127, 225, 1544, 1955, 4119
HincII GTYRAC 1 cut(s) 3516
HindII GTYRAC 1 cut(s) 3516
HindIII AAGCTT 2 cut(s) 2717, 3108
HpaII CCGG 2 cut(s) 261, 3923
HphI GGTGA 7 cut(s) 25, 1124, 1496, 2978, 3596, 3605, 3986
Hpy99I CGWCG 3 cut(s) 204, 3076, 3793
HpyCH4IV ACGT 7 cut(s) 1699, 1807, 2035, 3021, 3074, 3313, 3853
HpySE526I ACGT 7 cut(s) 1699, 1807, 2035, 3021, 3074, 3313, 3853
Hsp92I GRCGYC 1 cut(s) 3021
HspAI GCGC 6 cut(s) 119, 127, 225, 1544, 1955, 4119
Ksp22I TGATCA 1 cut(s) 4173
LmnI GCTCC 7 cut(s) 497, 691, 804, 1743, 2347, 3302, 3839
Lsp1109I GCAGC 6 cut(s) 133, 758, 1589, 1675, 2259, 2767
LweI GCATC 8 cut(s) 298, 1468, 1570, 1594, 1912, 3181, 3546, 4018
MaeI CTAG 8 cut(s) 333, 1311, 1926, 2507, 2633, 3095, 3185, 3915
MaeII ACGT 7 cut(s) 1699, 1807, 2035, 3021, 3074, 3313, 3853
MflI RGATCY 7 cut(s) 241, 1528, 1848, 2158, 2389, 2542, 3979
MhlI GDGCHC 4 cut(s) 466, 801, 1210, 2200
MlsI TGGCCA 2 cut(s) 188, 2356
MluNI TGGCCA 2 cut(s) 188, 2356
MlyI GAGTC 5 cut(s) 2174, 2678, 3077, 3779, 3884
MmeI TCCRAC 9 cut(s) 171, 1218, 1783, 2200, 2857, 3280, 3334, 3771, 3817
Mox20I TGGCCA 2 cut(s) 188, 2356
Mph1103I ATGCAT 3 cut(s) 1563, 2578, 3751
MroXI GAANNNNTTC 3 cut(s) 2037, 2976, 3399
MscI TGGCCA 2 cut(s) 188, 2356
MslI CAYNNNNRTG 3 cut(s) 384, 2151, 2498
Msp20I TGGCCA 2 cut(s) 188, 2356
MspA1I CMGCKG 2 cut(s) 1666, 2780
MspCI CTTAAG 1 cut(s) 3859
MspI CCGG 2 cut(s) 261, 3923
MspR9I CCNGG 9 cut(s) 262, 321, 778, 1265, 2265, 2516, 2648, 3582, 3849
Mva1269I GAATGC 4 cut(s) 69, 1386, 3576, 3749
MvaI CCWGG 8 cut(s) 321, 778, 1265, 2265, 2516, 2648, 3582, 3849
MvnI CGCG 2 cut(s) 127, 4119
NciI CCSGG 1 cut(s) 262
NcoI CCATGG 1 cut(s) 189
NdeI CATATG 1 cut(s) 355
NlaIV GGNNCC 6 cut(s) 243, 367, 442, 493, 846, 1242
NmeAIII GCCGAG 1 cut(s) 3829
NmuCI GTSAC 3 cut(s) 1135, 1484, 1976
NsiI ATGCAT 3 cut(s) 1563, 2578, 3751
NspI RCATGY 3 cut(s) 971, 2294, 2587
OliI CACNNNNGTG 1 cut(s) 2151
PasI CCCWGGG 1 cut(s) 1264
PceI AGGCCT 1 cut(s) 4005
PciI ACATGT 1 cut(s) 2290
PctI GAATGC 4 cut(s) 69, 1386, 3576, 3749
PdmI GAANNNNTTC 3 cut(s) 2037, 2976, 3399
PfoI TCCNGGA 2 cut(s) 2263, 3847
PinAI ACCGGT 1 cut(s) 3922
PkrI GCNGC 9 cut(s) 123, 126, 596, 748, 883, 1579, 1665, 2274, 2782
PleI GAGTC 5 cut(s) 2173, 2677, 3077, 3779, 3884
PmaCI CACGTG 1 cut(s) 1700
PmlI CACGTG 1 cut(s) 1700
PpsI GAGTC 5 cut(s) 2173, 2677, 3077, 3779, 3884
Ppu21I YACGTR 2 cut(s) 1700, 3314
PpuMI RGGWCCY 1 cut(s) 1120
PscI ACATGT 1 cut(s) 2290
Psp124BI GAGCTC 2 cut(s) 466, 801
Psp5II RGGWCCY 1 cut(s) 1120
Psp6I CCWGG 8 cut(s) 319, 776, 1263, 2263, 2514, 2646, 3580, 3847
PspCI CACGTG 1 cut(s) 1700
PspEI GGTNACC 1 cut(s) 3908
PspFI CCCAGC 2 cut(s) 2468, 3223
PspGI CCWGG 8 cut(s) 319, 776, 1263, 2263, 2514, 2646, 3580, 3847
PspN4I GGNNCC 6 cut(s) 243, 367, 442, 493, 846, 1242
PspPI GGNCC 4 cut(s) 1120, 2471, 2728, 3945
PspPPI RGGWCCY 1 cut(s) 1120
PstNI CAGNNNCTG 5 cut(s) 40, 326, 653, 2350, 2996
PsuI RGATCY 7 cut(s) 241, 1528, 1848, 2158, 2389, 2542, 3979
PvuII CAGCTG 2 cut(s) 1666, 2780
RseI CAYNNNNRTG 3 cut(s) 384, 2151, 2498
SacI GAGCTC 2 cut(s) 466, 801
SatI GCNGC 9 cut(s) 122, 125, 595, 747, 882, 1578, 1664, 2273, 2781
Sau96I GGNCC 4 cut(s) 1120, 2471, 2728, 3945
SchI GAGTC 5 cut(s) 2174, 2678, 3077, 3779, 3884
ScrFI CCNGG 9 cut(s) 262, 321, 778, 1265, 2265, 2516, 2648, 3582, 3849
SduI GDGCHC 4 cut(s) 466, 801, 1210, 2200
SfaNI GCATC 8 cut(s) 298, 1468, 1570, 1594, 1912, 3181, 3546, 4018
SfcI CTRYAG 3 cut(s) 540, 981, 1102
SinI GGWCC 3 cut(s) 1120, 2728, 3945
SmiMI CAYNNNNRTG 3 cut(s) 384, 2151, 2498
SmlI CTYRAG 3 cut(s) 2162, 2333, 3859
SmoI CTYRAG 3 cut(s) 2162, 2333, 3859
SseBI AGGCCT 1 cut(s) 4005
SspI AATATT 2 cut(s) 3091, 3319
SspMI CTAG 8 cut(s) 333, 1311, 1926, 2507, 2633, 3095, 3185, 3915
SstI GAGCTC 2 cut(s) 466, 801
StuI AGGCCT 1 cut(s) 4005
StyD4I CCNGG 9 cut(s) 260, 319, 776, 1263, 2263, 2514, 2646, 3580, 3847
StyI CCWWGG 8 cut(s) 189, 369, 876, 1053, 1423, 2707, 2731, 3575
TaiI ACGT 7 cut(s) 1702, 1810, 2038, 3024, 3077, 3316, 3856
TaqI TCGA 5 cut(s) 1771, 1840, 2806, 3496, 4134
TatI WGTACW 5 cut(s) 229, 711, 1255, 2224, 2487
TauI GCSGC 3 cut(s) 127, 597, 884
TscAI CASTG 4 cut(s) 117, 2200, 2914, 4021
TseFI GTSAC 3 cut(s) 1135, 1484, 1976
TseI GCWGC 6 cut(s) 121, 746, 1577, 1663, 2272, 2780
Tsp45I GTSAC 3 cut(s) 1135, 1484, 1976
TspGWI ACGGA 5 cut(s) 254, 490, 1072, 1798, 2756
TspRI CASTG 4 cut(s) 117, 2200, 2914, 4021
Vha464I CTTAAG 1 cut(s) 3859
VpaK11BI GGWCC 3 cut(s) 1120, 2728, 3945
XagI CCTNNNNNAGG 1 cut(s) 2321
XapI RAATTY 6 cut(s) 152, 669, 1836, 2976, 3357, 3444
XbaI TCTAGA 1 cut(s) 2506
XceI RCATGY 3 cut(s) 971, 2294, 2587
XmiI GTMKAC 2 cut(s) 1796, 2925
XmnI GAANNNNTTC 3 cut(s) 2037, 2976, 3399
XspI CTAG 8 cut(s) 333, 1311, 1926, 2507, 2633, 3095, 3185, 3915
ZraI GACGTC 1 cut(s) 3022
Zsp2I ATGCAT 3 cut(s) 1563, 2578, 3751
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.