Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0004755

Physical Location & Seq

Genomic Coordinates Reverse (-)

32265221 .. 32270384

Gene Structure 5164 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g34560.t3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1695 bp

ATGGAAGGAGAGAGAGCCGAGAAGAAGAGGCCGCTTGCTATTGACTTCAACAAGCTCTTGGATGGCAATGACGATTTTGACCCGCCGGAGGTTCTCAGAGTGAAGAAGACCAAGCCCGTCACCATCGCCGCCGACGAGACACCGAAACGGGCGGCGATGGGCGGCGGCGATGAGCTAAGGGATGAGGAACTTGAGAGGGTGAGCAATAAGGACCTGGATGATTTGATTGGGCGGCAAAGAGCACATGTGAAGATGTTGGCTCCAAAATTGCCAGACAAAGGCGAAAAGCTGAGGGTTCGTTTGAAGAAAATGGAGGATGAGCAGCAGAGGAGGATTGAACACCCAGAGAGGTATATCAGTATGGTGGAGAAGCCTACCGGAGCGACCAGCTCTGCAGGTGTTTCTAGTGGCTTCAGAGAGGAGGCTACATCATCTCCAGGCGAGCGAGGGGCTTCATTTTTAAAACTCTTTTCTCAAAAGCTGGATGACGATAGAGATTCTAACAAGGGAAATGCATTTGAAAGAGAATTTTCAACTTTGGGTCACTGCAACCGCCAGAAAAAGAACAATAATGGAGCATTCGTGCCAAAGGGGGCACAGAAAAGTCGATCTTCATCAAGACAATTCCCTTTTCAAAGCCCTAGCAACTTCTATCGTGATAAAGATAAAACTTCTCTCTCAAATGGTGATCGTAAAGGCAAAGCTTCTTCCAATAACTCAGGTTATCAGTGTGAGGAAAACTCCTCCAGCAGCTTTGCAAAAAAGGAGGATCATTATCAACTTCGGAATAGGACCAGGAAGAGTCAGACCATTTTACTTGTGGATGAGGAGGAACCTGAGTTCACAGACACCACAGAGCAAACAGAAGAAATTCCTGAGAGCATGAAAGACTCCAGAATCTACTATCCATCAAGGGATGATCCACATTCCGTTGAAATTTGTTATGCAGACACTAAGTGCCTTGATCCTCAAAGCTACCTGACATCAGCAATTATGAATTTCTACATACGGTATTTGCATCAACAAGCATCCCCTACAGACACGGGAAGTTGTGCATTTCATTTCTTCAATACCTTTTTTTACAATAAGCTCAAAGATGCTGTATCAGAAAAGGGAGCTAACAAGGATAACCGCTTTGTCAAGCTTAGAAGGTGGTGGAAGGGTGTTGATATGTTTCAGAAGGCTTACATACTTATTCCTATAAATGAAGACGTTCATTGGAGCCTTGTCATTATCTGTATCCCTGACAAGGAAGAAGAATCAGGACCACTTGTTCTTCATTTAGACTCATTAGGACTCCATTGTAGCAAATCCATTTTTCGAATTATTAAAAGCTTTTTAAAAGAAGAATGGTGCTATTTGGATGAAGTTGATATTTTAGATCTAACAGTTTCAGACTGTTGGAAACGACTTCCTGACCATATTGAGGAGAAAAGACTTCCGGTCCCGCAACAGAAAAATGACTATGACTGTGGTCTTTTTGTACTCTTGTTTATGGAACACTTCATAGAAGACGCTCCTGAAAGGCTAAAAAAGAAAGATTTATCAATGTTTGGCAGGCAGTGGTTCCAACCTCAAGAGGCTTCTAGGTTGAGAAACAAAATCCGTAAGTTACTCAAGGAAGAATTTCAAAATGCATCCAAGTCTAACCGCTCTAAAGAACCTTCTCCTCTGAGTGATGGTTCTGAAGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006464 GO:0006508 GO:0006807 GO:0006950 GO:0006970 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009628 GO:0009651 GO:0009791 GO:0009987 GO:0010228 GO:0016787 GO:0016926 GO:0016929 GO:0018193 GO:0018205 GO:0019538 GO:0019783 GO:0022414 GO:0032501 GO:0032502 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0061458 GO:0070011 GO:0070122 GO:0070137 GO:0070138 GO:0070139 GO:0070140 GO:0070646 GO:0070647 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_C48

Protein Analysis

565

Amino Acids

65.09

Weight (kDa)

6.52

Isoelectric Point (pI)

54.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_C48	PF02902	326 - 370	2.9e-06	Ulp1 protease family, C-terminal catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004755)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G10570 AT1G10570 AT1G10570 AT1G60220
fragaria_vesca	FvH4_4g34560 FvH4_4g34560 FvH4_4g34560 FvH4_4g34560 FvH4_4g34560 FvH4_4g34560 FvH4_4g34560
malus_domestica	MD13G1091500.v1.1
prunus_persica	Prupe.1G254600_v2.0.a1 Prupe.1G254600_v2.0.a1 Prupe.1G254600_v2.0.a1 Prupe.1G254600_v2.0.a1
pyrus_communis	pycom13g07950
rosa_chinensis	RchiOBHm_Chr3g0455541 RchiOBHm_Chr4g0443941
rosa_laevigata	RLG00000005875
rosa_multiflora	Rmu_sc0011390.1_g000004
rosa_roxburghii	Rroxscaffold_5G00384480
rosa_rugosa	Rorug03G0008300 Rorug04G0348400 Rorug04G0348500
rosa_samantha	Rh4AG409000 Rh4BG420100 Rh4CG434100 Rh4DG415100
rosa_wichuraiana	Rw3G005410 Rw4G035130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	386
Acc36I	ACCTGC	1 cut(s)	386
AccBSI	CCGCTC	1 cut(s)	1653
AclWI	GGATC	3 cut(s)	777, 914, 959
AcsI	RAATTY	5 cut(s)	527, 870, 936, 997, 1625
AcuI	CTGAAG	1 cut(s)	397
AdeI	CACNNNGTG	1 cut(s)	957
AfaI	GTAC	1 cut(s)	1485
AfiI	CCNNNNNNNGG	4 cut(s)	88, 278, 1249, 1426
AflIII	ACRYGT	1 cut(s)	244
AgsI	TTSAA	9 cut(s)	49, 304, 338, 521, 534, 635, 935, 1069, 1631
AhdI	GACNNNNNGTC	1 cut(s)	1442
AjnI	CCWGG	3 cut(s)	213, 436, 794
AloI	GAACNNNNNNTCC	4 cut(s)	824, 856, 1257, 1289
Alw21I	GWGCWC	1 cut(s)	244
Alw26I	GTCTC	1 cut(s)	131
AlwI	GGATC	3 cut(s)	777, 914, 959
AoxI	GGCC	1 cut(s)	29
ApeKI	GCWGC	2 cut(s)	322, 750
ApoI	RAATTY	5 cut(s)	527, 870, 936, 997, 1625
Asp700I	GAANNNNTTC	3 cut(s)	870, 1212, 1626
AspS9I	GGNCC	4 cut(s)	211, 792, 1265, 1444
AsuHPI	GGTGA	3 cut(s)	112, 211, 698
AsuII	TTCGAA	1 cut(s)	1321
AvaII	GGWCC	4 cut(s)	211, 792, 1265, 1444
BaeGI	GKGCMC	1 cut(s)	598
BbsI	GAAGAC	3 cut(s)	113, 1215, 1518
Bbv12I	GWGCWC	1 cut(s)	244
BbvCI	CCTCAGC	1 cut(s)	290
BbvI	GCAGC	2 cut(s)	334, 762
BccI	CCATC	5 cut(s)	56, 131, 151, 916, 1673
BciT130I	CCWGG	3 cut(s)	215, 438, 796
BciVI	GTATCC	1 cut(s)	1250
BcoDI	GTCTC	1 cut(s)	131
BfaI	CTAG	3 cut(s)	405, 642, 1587
BfmI	CTRYAG	2 cut(s)	393, 1035
BfuAI	ACCTGC	1 cut(s)	386
BfuI	GTATCC	1 cut(s)	1250
BglII	AGATCT	1 cut(s)	1381
BisI	GCNGC	8 cut(s)	32, 129, 153, 163, 166, 233, 323, 751
BlsI	GCNGC	8 cut(s)	33, 130, 154, 164, 167, 234, 324, 752
Bme1390I	CCNGG	3 cut(s)	215, 438, 796
Bme18I	GGWCC	4 cut(s)	211, 792, 1265, 1444
BmeRI	GACNNNNNGTC	1 cut(s)	1442
BmgT120I	GGNCC	4 cut(s)	211, 792, 1265, 1444
BmiI	GGNNCC	5 cut(s)	261, 834, 1223, 1446, 1568
BmrFI	CCNGG	3 cut(s)	215, 438, 796
BmsI	GCATC	4 cut(s)	1027, 1037, 1087, 1646
BoxI	GACNNNNGTC	1 cut(s)	1473
BpiI	GAAGAC	3 cut(s)	113, 1215, 1518
BplI	GAGNNNNNCTC	2 cut(s)	725, 757
BpmI	CTGGAG	3 cut(s)	420, 730, 877
Bpu10I	CCTNAGC	2 cut(s)	176, 290
Bpu14I	TTCGAA	1 cut(s)	1321
BpuEI	CTTGAG	3 cut(s)	212, 1560, 1601
BsaBI	GATNNNNATC	2 cut(s)	613, 774
BsaWI	WCCGGW	2 cut(s)	377, 1441
BsaXI	ACNNNNNCTCC	4 cut(s)	418, 448, 567, 597
Bsc4I	CCNNNNNNNGG	4 cut(s)	88, 278, 1249, 1426
Bse3DI	GCAATG	1 cut(s)	73
Bse8I	GATNNNNATC	2 cut(s)	613, 774
BseBI	CCWGG	3 cut(s)	215, 438, 796
BseJI	GATNNNNATC	2 cut(s)	613, 774
BseLI	CCNNNNNNNGG	4 cut(s)	88, 278, 1249, 1426
BseMI	GCAATG	1 cut(s)	73
BseMII	CTCAG	6 cut(s)	109, 281, 732, 828, 867, 1664
BseRI	GAGGAG	6 cut(s)	343, 434, 733, 842, 1442, 1659
BseSI	GKGCMC	1 cut(s)	598
BseXI	GCAGC	2 cut(s)	334, 762
BshFI	GGCC	1 cut(s)	31
BsiHKAI	GWGCWC	1 cut(s)	244
BsiSI	CCGG	3 cut(s)	86, 378, 1442
BslFI	GGGAC	1 cut(s)	1430
BslI	CCNNNNNNNGG	4 cut(s)	88, 278, 1249, 1426
BsmAI	GTCTC	1 cut(s)	131
BsmFI	GGGAC	1 cut(s)	1430
BsmI	GAATGC	1 cut(s)	578
BsnI	GGCC	1 cut(s)	31
Bsp119I	TTCGAA	1 cut(s)	1321
Bsp1286I	GDGCHC	2 cut(s)	244, 598
Bsp143I	GATC	6 cut(s)	608, 688, 769, 919, 964, 1381
BspANI	GGCC	1 cut(s)	31
BspCNI	CTCAG	6 cut(s)	108, 282, 731, 829, 868, 1665
BspLI	GGNNCC	5 cut(s)	261, 834, 1223, 1446, 1568
BspMAI	CTGCAG	1 cut(s)	397
BspMI	ACCTGC	1 cut(s)	386
BspPI	GGATC	3 cut(s)	777, 914, 959
BspT104I	TTCGAA	1 cut(s)	1321
BsrBI	CCGCTC	1 cut(s)	1653
BsrDI	GCAATG	1 cut(s)	73
BssMI	GATC	6 cut(s)	608, 688, 769, 919, 964, 1381
Bst2UI	CCWGG	3 cut(s)	215, 438, 796
Bst4CI	ACNGT	4 cut(s)	1011, 1390, 1400, 1472
Bst6I	CTCTTC	2 cut(s)	20, 794
BstBI	TTCGAA	1 cut(s)	1321
BstC8I	GCNNGC	3 cut(s)	36, 443, 1559
BstDEI	CTNAG	9 cut(s)	95, 176, 290, 718, 837, 876, 954, 1145, 1673
BstKTI	GATC	6 cut(s)	611, 691, 772, 922, 967, 1384
BstMAI	GTCTC	1 cut(s)	131
BstMBI	GATC	6 cut(s)	608, 688, 769, 919, 964, 1381
BstNI	CCWGG	3 cut(s)	215, 438, 796
BstNSI	RCATGY	1 cut(s)	248
BstPAI	GACNNNNGTC	1 cut(s)	1473
BstSCI	CCNGG	3 cut(s)	213, 436, 794
BstSFI	CTRYAG	2 cut(s)	393, 1035
BstSLI	GKGCMC	1 cut(s)	598
BstV1I	GCAGC	2 cut(s)	334, 762
BstV2I	GAAGAC	3 cut(s)	113, 1215, 1518
BstX2I	RGATCY	1 cut(s)	1381
BstYI	RGATCY	1 cut(s)	1381
BsuI	GTATCC	1 cut(s)	1250
BsuRI	GGCC	1 cut(s)	31
BtgZI	GCGATG	3 cut(s)	109, 170, 183
BtsI	GCAGTG	2 cut(s)	544, 1568
BtsIMutI	CAGTG	3 cut(s)	544, 734, 1568
BveI	ACCTGC	1 cut(s)	386
Cac8I	GCNNGC	3 cut(s)	36, 443, 1559
Cfr13I	GGNCC	4 cut(s)	211, 792, 1265, 1444
CseI	GACGC	1 cut(s)	1523
Csp6I	GTAC	1 cut(s)	1484
CviAII	CATG	2 cut(s)	245, 883
CviQI	GTAC	1 cut(s)	1484
DdeI	CTNAG	9 cut(s)	95, 176, 290, 718, 837, 876, 954, 1145, 1673
DpnI	GATC	6 cut(s)	610, 690, 771, 921, 966, 1383
DpnII	GATC	6 cut(s)	608, 688, 769, 919, 964, 1381
DraI	TTTAAA	2 cut(s)	462, 1341
DraIII	CACNNNGTG	1 cut(s)	957
DriI	GACNNNNNGTC	1 cut(s)	1442
Eam1104I	CTCTTC	2 cut(s)	20, 794
Eam1105I	GACNNNNNGTC	1 cut(s)	1442
EarI	CTCTTC	2 cut(s)	20, 794
Eco47I	GGWCC	4 cut(s)	211, 792, 1265, 1444
Eco57I	CTGAAG	1 cut(s)	397
EcoO109I	RGGNCCY	1 cut(s)	211
EcoRII	CCWGG	3 cut(s)	213, 436, 794
EcoT22I	ATGCAT	2 cut(s)	517, 1639
FaeI	CATG	2 cut(s)	248, 886
FalI	AAGNNNNNCTT	2 cut(s)	595, 627
FaqI	GGGAC	1 cut(s)	1430
FatI	CATG	2 cut(s)	244, 882
FauI	CCCGC	2 cut(s)	90, 1455
Fnu4HI	GCNGC	8 cut(s)	32, 129, 153, 163, 166, 233, 323, 751
Fsp4HI	GCNGC	8 cut(s)	32, 129, 153, 163, 166, 233, 323, 751
FspBI	CTAG	3 cut(s)	405, 642, 1587
GluI	GCNGC	8 cut(s)	32, 129, 153, 163, 166, 233, 323, 751
GsuI	CTGGAG	3 cut(s)	420, 730, 877
HaeIII	GGCC	1 cut(s)	31
HapII	CCGG	3 cut(s)	86, 378, 1442
HgaI	GACGC	1 cut(s)	1523
Hin1II	CATG	2 cut(s)	248, 886
HindIII	AAGCTT	3 cut(s)	702, 1142, 1333
HinfI	GANTC	7 cut(s)	497, 802, 890, 897, 1259, 1286, 1296
HpaII	CCGG	3 cut(s)	86, 378, 1442
HphI	GGTGA	3 cut(s)	112, 211, 698
Hpy166II	GTNNAC	1 cut(s)	843
Hpy188I	TCNGA	9 cut(s)	98, 416, 786, 807, 1108, 1179, 1396, 1674, 1687
Hpy188III	TCNNGA	8 cut(s)	618, 656, 875, 894, 1263, 1415, 1520, 1577
Hpy8I	GTNNAC	1 cut(s)	843
Hpy99I	CGWCG	1 cut(s)	137
HpyAV	CCTTC	4 cut(s)	1143, 1153, 1174, 1674
HpyCH4III	ACNGT	4 cut(s)	1011, 1390, 1400, 1472
HpyCH4IV	ACGT	1 cut(s)	1212
HpyCH4V	TGCA	8 cut(s)	395, 515, 549, 758, 947, 1018, 1055, 1637
HpyF3I	CTNAG	9 cut(s)	95, 176, 290, 718, 837, 876, 954, 1145, 1673
HpySE526I	ACGT	1 cut(s)	1212
Hsp92II	CATG	2 cut(s)	248, 886
Kzo9I	GATC	6 cut(s)	608, 688, 769, 919, 964, 1381
LmnI	GCTCC	6 cut(s)	265, 380, 575, 1115, 1221, 1522
Lsp1109I	GCAGC	2 cut(s)	334, 762
LweI	GCATC	4 cut(s)	1027, 1037, 1087, 1646
MaeI	CTAG	3 cut(s)	405, 642, 1587
MaeII	ACGT	1 cut(s)	1212
MaeIII	GTNAC	3 cut(s)	118, 542, 1611
MalI	GATC	6 cut(s)	610, 690, 771, 921, 966, 1383
MbiI	CCGCTC	1 cut(s)	1653
MboI	GATC	6 cut(s)	608, 688, 769, 919, 964, 1381
MflI	RGATCY	1 cut(s)	1381
MhlI	GDGCHC	2 cut(s)	244, 598
MluCI	AATT	9 cut(s)	266, 527, 623, 870, 936, 990, 997, 1323, 1625
MlyI	GAGTC	4 cut(s)	811, 884, 1280, 1290
MmeI	TCCRAC	2 cut(s)	1382, 1594
Mph1103I	ATGCAT	2 cut(s)	517, 1639
MroXI	GAANNNNTTC	3 cut(s)	870, 1212, 1626
MseI	TTAA	3 cut(s)	461, 1329, 1340
MspI	CCGG	3 cut(s)	86, 378, 1442
MspR9I	CCNGG	3 cut(s)	215, 438, 796
Mva1269I	GAATGC	1 cut(s)	578
MvaI	CCWGG	3 cut(s)	215, 438, 796
NdeII	GATC	6 cut(s)	608, 688, 769, 919, 964, 1381
NlaIII	CATG	2 cut(s)	248, 886
NlaIV	GGNNCC	5 cut(s)	261, 834, 1223, 1446, 1568
NmeAIII	GCCGAG	1 cut(s)	43
NmuCI	GTSAC	2 cut(s)	118, 542
NsiI	ATGCAT	2 cut(s)	517, 1639
NspI	RCATGY	1 cut(s)	248
NspV	TTCGAA	1 cut(s)	1321
PaqCI	CACCTGC	1 cut(s)	386
PciI	ACATGT	1 cut(s)	244
PcsI	WCGNNNNNNNCGW	1 cut(s)	132
PctI	GAATGC	1 cut(s)	578
PdmI	GAANNNNTTC	3 cut(s)	870, 1212, 1626
PfeI	GAWTC	3 cut(s)	497, 897, 1259
PkrI	GCNGC	8 cut(s)	33, 130, 154, 164, 167, 234, 324, 752
PleI	GAGTC	4 cut(s)	810, 884, 1280, 1290
PpsI	GAGTC	4 cut(s)	810, 884, 1280, 1290
PpuMI	RGGWCCY	1 cut(s)	211
PscI	ACATGT	1 cut(s)	244
PshAI	GACNNNNGTC	1 cut(s)	1473
Psp5II	RGGWCCY	1 cut(s)	211
Psp6I	CCWGG	3 cut(s)	213, 436, 794
PspGI	CCWGG	3 cut(s)	213, 436, 794
PspN4I	GGNNCC	5 cut(s)	261, 834, 1223, 1446, 1568
PspPI	GGNCC	4 cut(s)	211, 792, 1265, 1444
PspPPI	RGGWCCY	1 cut(s)	211
PstI	CTGCAG	1 cut(s)	397
PsuI	RGATCY	1 cut(s)	1381
RsaI	GTAC	1 cut(s)	1485
RsaNI	GTAC	1 cut(s)	1484
SaqAI	TTAA	3 cut(s)	461, 1329, 1340
SatI	GCNGC	8 cut(s)	32, 129, 153, 163, 166, 233, 323, 751
Sau3AI	GATC	6 cut(s)	608, 688, 769, 919, 964, 1381
Sau96I	GGNCC	4 cut(s)	211, 792, 1265, 1444
SchI	GAGTC	4 cut(s)	811, 884, 1280, 1290
ScrFI	CCNGG	3 cut(s)	215, 438, 796
SduI	GDGCHC	2 cut(s)	244, 598
SfaNI	GCATC	4 cut(s)	1027, 1037, 1087, 1646
SfcI	CTRYAG	2 cut(s)	393, 1035
SfuI	TTCGAA	1 cut(s)	1321
SinI	GGWCC	4 cut(s)	211, 792, 1265, 1444
SmlI	CTYRAG	3 cut(s)	191, 1575, 1616
SmoI	CTYRAG	3 cut(s)	191, 1575, 1616
Sse9I	AATT	9 cut(s)	266, 527, 623, 870, 936, 990, 997, 1323, 1625
SspMI	CTAG	3 cut(s)	405, 642, 1587
StyD4I	CCNGG	3 cut(s)	213, 436, 794
TaaI	ACNGT	4 cut(s)	1011, 1390, 1400, 1472
TaiI	ACGT	1 cut(s)	1215
TaqI	TCGA	2 cut(s)	607, 1321
TasI	AATT	9 cut(s)	266, 527, 623, 870, 936, 990, 997, 1323, 1625
TatI	WGTACW	1 cut(s)	1483
TauI	GCSGC	6 cut(s)	34, 131, 155, 165, 168, 235
TfiI	GAWTC	3 cut(s)	497, 897, 1259
Tru1I	TTAA	3 cut(s)	461, 1329, 1340
Tru9I	TTAA	3 cut(s)	461, 1329, 1340
TscAI	CASTG	3 cut(s)	551, 734, 1568
TseFI	GTSAC	2 cut(s)	118, 542
TseI	GCWGC	2 cut(s)	322, 750
Tsp45I	GTSAC	2 cut(s)	118, 542
TspGWI	ACGGA	2 cut(s)	919, 1595
TspRI	CASTG	3 cut(s)	551, 734, 1568
VpaK11BI	GGWCC	4 cut(s)	211, 792, 1265, 1444
XapI	RAATTY	5 cut(s)	527, 870, 936, 997, 1625
XceI	RCATGY	1 cut(s)	248
XcmI	CCANNNNNNNNNTGG	1 cut(s)	817
XmnI	GAANNNNTTC	3 cut(s)	870, 1212, 1626
XspI	CTAG	3 cut(s)	405, 642, 1587
Zsp2I	ATGCAT	2 cut(s)	517, 1639

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.