RLG00000005873

Equilibrative nucleotide transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0014468
Physical Location & Seq
Forward (+)
2485491 .. 2486982
1492 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005873

Sequence Viewer

Length: 1287 bp
ATGGGTTTCGCCGCCAAAACCCCCGAAGAAAACTCCGAATCGAGTCTCCTCCTGCCCACCACCACCCCCACCCCCACCCCCGACGGCGCCGCCGCACCCAATAAAGTCCCAAACGACCCTTTCCATCTGACCTACATCATCTACTTCACTCTGGGCCTTGGCTACCTCCTCCCATGGAACGCTTTCATCACAGCCGTTGATTACTTCACCTACCTCTACCCCGACGCCAGCGTCGACCGTATATTCTCCGTCGCGTACATGCTCGTCGGCCTCGTCGTCCTTGTGATTATCGTCTTCTACGCCCAGAAGTCCGACGCCTACGTCCGGATCAACGTCGGCCTCGCCCTGTTTGTGGTGTCGCTGCTCATCGTGCCGGTGCTGGACTTGGTCTACATCCAGGGCCGGGTCGGGTTGTACGCCGGGTTTTACGTCACGGTTGCCGCGGTTGCGCTTTCGGGTCTGGCTGATGCTCTGGTCCAAGGCTCGCTGATCGGCGCAGCGGGTGAGTTGCCTGAGAAGTATATGCAGGCCATTGTGGCTGGCACTGCTGGTTCAGGAGTGCTTGTTTCGTTTTTGAGAATCCTAACGAAGGCTGTATATCCGCAAACTGGTGAGGGACTGAGGAAAAGTGCAAATCTCTACTTTGCTGTGGGGATAGTAGTTATGGTCATATGCATTGTTTTCTACAATGTGGCACCGAGGATTCCGGTTATGAAGTACTATGCAGAGTTGAAGATTCAGACTATAAATGAGGAGAAAGAATTGAAAGGTCCTCTGACGGGTTCTGTGCTGAGATCGACTCTATGGGGTGTAGTTGGGAGTGTCAAGTGGTATGGTATCGGCATTCTGCTTATCTACATTGTGACTTTGGCAATATTTCCTGGGTATATTACCGAAGATGTGCACTCTGATCTTCTCAAGGATTGGTACGCGGTCATCCTTATTACTGGTTACAATGTGTTTGATCTGGTTGGCAAGGCTCTGACTTCAGCATATCTCCTTGAAAATTCCAAGGTTGCAATCGGCGGCACAGTTGCTAGATTGCTATTCTTTCCTCTGTTCTATGGGTGCTTGCACGGCCCGGCATTCTTCAGAACAGAGATTCCAGTGACAATACTGACTTGCCTTTTGGGCCTTACTAATGGCTACTTGACGAGTGTGTTGATGATTTTGGCTCCCAAAGTTGTCCAATTACAACATGCCGAGACTGCAGGGATTGTGATTGTCTTGTTCTTGGTTCTGGGTTTGGCTGCCGGTTCACTTGTATCTTGGTTTTGGGTCATCTGA

Protein Analysis

429

Amino Acids

46.36

Weight (kDa)

6.11

Isoelectric Point (pI)

26.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nucleoside_tran PF01733 147 - 428 1.5e-76 Nucleoside transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014468)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G70330
fragaria_vesca FvH4_4g34590
malus_domestica MD13G1091100.v1.1 MD16G1091600.v1.1
prunus_persica Prupe.1G254800_v2.0.a1
pyrus_communis pycom13g07930 pycom16g07820
rosa_chinensis RchiOBHm_Chr4g0443961
rosa_laevigata RLG00000005873
rosa_multiflora Rmu_sc0011390.1_g000001
rosa_roxburghii Rroxscaffold_5G00384500
rosa_rugosa Rorug04G0348500
rosa_samantha Rh4AG409200 Rh4BG420300 Rh4CG434300 Rh4DG415300
rosa_wichuraiana Rw4G035150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 230, 320
AccB1I GGYRCC 2 cut(s) 86, 694
AccI GTMKAC 2 cut(s) 234, 390
AccII CGCG 3 cut(s) 254, 443, 932
AccIII TCCGGA 1 cut(s) 324
AciI CCGC 9 cut(s) 12, 90, 93, 441, 443, 500, 602, 932, 1026
AclWI GGATC 1 cut(s) 335
AcsI RAATTY 1 cut(s) 1006
AcuI CTGAAG 2 cut(s) 972, 1075
AcyI GRCGYC 3 cut(s) 87, 225, 315
AfaI GTAC 4 cut(s) 257, 416, 719, 929
AfiI CCNNNNNNNGG 6 cut(s) 324, 352, 403, 589, 608, 779
AgsI TTSAA 3 cut(s) 733, 766, 1004
AjnI CCWGG 2 cut(s) 396, 880
AloI GAACNNNNNNTCC 2 cut(s) 1087, 1119
Alw21I GWGCWC 1 cut(s) 906
Alw26I GTCTC 2 cut(s) 50, 1199
Alw44I GTGCAC 1 cut(s) 902
AlwI GGATC 1 cut(s) 335
Aor13HI TCCGGA 1 cut(s) 324
AoxI GGCC 7 cut(s) 154, 268, 337, 400, 528, 1078, 1132
ApaLI GTGCAC 1 cut(s) 902
ApeKI GCWGC 3 cut(s) 361, 497, 1250
ApoI RAATTY 1 cut(s) 1006
ArsI GACNNNNNNTTYG 2 cut(s) 1111, 1143
Asp700I GAANNNNTTC 1 cut(s) 182
AspLEI GCGC 3 cut(s) 89, 451, 497
AspS9I GGNCC 6 cut(s) 154, 400, 475, 770, 1079, 1132
AsuC2I CCSGG 3 cut(s) 404, 421, 1082
AsuHPI GGTGA 3 cut(s) 199, 515, 623
AvaII GGWCC 2 cut(s) 475, 770
BaeGI GKGCMC 1 cut(s) 906
BanI GGYRCC 2 cut(s) 86, 694
BbsI GAAGAC 1 cut(s) 286
Bbv12I GWGCWC 1 cut(s) 906
BbvI GCAGC 3 cut(s) 348, 509, 1237
BccI CCATC 1 cut(s) 132
BceAI ACGGC 3 cut(s) 100, 179, 1093
BciT130I CCWGG 2 cut(s) 398, 882
BcnI CCSGG 3 cut(s) 404, 421, 1082
BcoDI GTCTC 2 cut(s) 50, 1199
BfaI CTAG 1 cut(s) 1038
BfmI CTRYAG 1 cut(s) 1209
BfoI RGCGCY 1 cut(s) 90
BglI GCCNNNNNGGC 2 cut(s) 536, 1131
BisI GCNGC 8 cut(s) 12, 90, 93, 362, 441, 498, 1027, 1251
BlsI GCNGC 8 cut(s) 13, 91, 94, 363, 442, 499, 1028, 1252
BmcAI AGTACT 1 cut(s) 719
Bme1390I CCNGG 5 cut(s) 398, 404, 421, 882, 1082
Bme18I GGWCC 2 cut(s) 475, 770
BmgT120I GGNCC 6 cut(s) 154, 400, 475, 770, 1079, 1132
BmiI GGNNCC 3 cut(s) 88, 696, 1176
BmrFI CCNGG 5 cut(s) 398, 404, 421, 882, 1082
BmsI GCATC 1 cut(s) 457
BpiI GAAGAC 1 cut(s) 286
BplI GAGNNNNNCTC 2 cut(s) 784, 816
BpuEI CTTGAG 1 cut(s) 902
BpuMI CCSGG 3 cut(s) 404, 421, 1082
BsaHI GRCGYC 3 cut(s) 87, 225, 315
BsaJI CCNNGG 8 cut(s) 157, 173, 397, 441, 478, 698, 881, 1011
BsaWI WCCGGW 2 cut(s) 324, 706
BsaXI ACNNNNNCTCC 2 cut(s) 549, 579
Bsc4I CCNNNNNNNGG 6 cut(s) 324, 352, 403, 589, 608, 779
Bse118I RCCGGY 2 cut(s) 373, 1253
Bse1I ACTGG 3 cut(s) 613, 952, 1106
BseAI TCCGGA 1 cut(s) 324
BseBI CCWGG 2 cut(s) 398, 882
BseDI CCNNGG 8 cut(s) 157, 173, 397, 441, 478, 698, 881, 1011
BseGI GGATG 2 cut(s) 393, 936
BseLI CCNNNNNNNGG 6 cut(s) 324, 352, 403, 589, 608, 779
BseMII CTCAG 3 cut(s) 504, 611, 782
BseNI ACTGG 3 cut(s) 613, 952, 1106
BseRI GAGGAG 3 cut(s) 38, 158, 767
BseSI GKGCMC 1 cut(s) 906
BseXI GCAGC 3 cut(s) 348, 509, 1237
Bsh1236I CGCG 3 cut(s) 254, 443, 932
Bsh1285I CGRYCG 1 cut(s) 238
BshFI GGCC 7 cut(s) 156, 270, 339, 402, 530, 1080, 1134
BshNI GGYRCC 2 cut(s) 86, 694
BsiEI CGRYCG 1 cut(s) 238
BsiHKAI GWGCWC 1 cut(s) 906
BsiSI CCGG 7 cut(s) 325, 374, 403, 420, 707, 1082, 1254
BslFI GGGAC 2 cut(s) 92, 630
BslI CCNNNNNNNGG 6 cut(s) 324, 352, 403, 589, 608, 779
BsmAI GTCTC 2 cut(s) 50, 1199
BsmFI GGGAC 2 cut(s) 92, 630
BsmI GAATGC 2 cut(s) 843, 1085
BsnI GGCC 7 cut(s) 156, 270, 339, 402, 530, 1080, 1134
Bsp1286I GDGCHC 1 cut(s) 906
Bsp13I TCCGGA 1 cut(s) 324
Bsp143I GATC 5 cut(s) 327, 489, 794, 910, 964
Bsp19I CCATGG 1 cut(s) 173
BspACI CCGC 9 cut(s) 12, 90, 93, 441, 443, 500, 602, 932, 1026
BspANI GGCC 7 cut(s) 156, 270, 339, 402, 530, 1080, 1134
BspCNI CTCAG 3 cut(s) 505, 612, 783
BspEI TCCGGA 1 cut(s) 324
BspFNI CGCG 3 cut(s) 254, 443, 932
BspLI GGNNCC 3 cut(s) 88, 696, 1176
BspMAI CTGCAG 1 cut(s) 1213
BspPI GGATC 1 cut(s) 335
BspT107I GGYRCC 2 cut(s) 86, 694
BsrFI RCCGGY 2 cut(s) 373, 1253
BsrI ACTGG 3 cut(s) 613, 952, 1106
BssAI RCCGGY 2 cut(s) 373, 1253
BssECI CCNNGG 8 cut(s) 157, 173, 397, 441, 478, 698, 881, 1011
BssMI GATC 5 cut(s) 327, 489, 794, 910, 964
BssNI GRCGYC 3 cut(s) 87, 225, 315
BssT1I CCWWGG 4 cut(s) 157, 173, 478, 1011
Bst2UI CCWGG 2 cut(s) 398, 882
Bst4CI ACNGT 3 cut(s) 239, 436, 1033
BstACI GRCGYC 3 cut(s) 87, 225, 315
BstC8I GCNNGC 5 cut(s) 229, 485, 528, 541, 1073
BstDEI CTNAG 3 cut(s) 513, 620, 791
BstDSI CCRYGG 2 cut(s) 173, 441
BstENI CCTNNNNNAGG 1 cut(s) 587
BstF5I GGATG 2 cut(s) 393, 936
BstFNI CGCG 3 cut(s) 254, 443, 932
BstH2I RGCGCY 1 cut(s) 90
BstHHI GCGC 3 cut(s) 89, 451, 497
BstKTI GATC 5 cut(s) 330, 492, 797, 913, 967
BstMAI GTCTC 2 cut(s) 50, 1199
BstMBI GATC 5 cut(s) 327, 489, 794, 910, 964
BstMCI CGRYCG 1 cut(s) 238
BstMWI GCNNNNNNNGC 7 cut(s) 370, 446, 536, 545, 1077, 1131, 1208
BstNI CCWGG 2 cut(s) 398, 882
BstNSI RCATGY 2 cut(s) 262, 1202
BstSCI CCNGG 5 cut(s) 396, 402, 419, 880, 1080
BstSFI CTRYAG 1 cut(s) 1209
BstSLI GKGCMC 1 cut(s) 906
BstUI CGCG 3 cut(s) 254, 443, 932
BstV1I GCAGC 3 cut(s) 348, 509, 1237
BstV2I GAAGAC 1 cut(s) 286
BsuRI GGCC 7 cut(s) 156, 270, 339, 402, 530, 1080, 1134
BtgI CCRYGG 2 cut(s) 173, 441
BtsCI GGATG 2 cut(s) 393, 936
BtsI GCAGTG 1 cut(s) 543
BtsIMutI CAGTG 2 cut(s) 543, 1113
Cac8I GCNNGC 5 cut(s) 229, 485, 528, 541, 1073
CfoI GCGC 3 cut(s) 89, 451, 497
Cfr10I RCCGGY 2 cut(s) 373, 1253
Cfr13I GGNCC 6 cut(s) 154, 400, 475, 770, 1079, 1132
Cfr42I CCGCGG 1 cut(s) 444
CseI GACGC 3 cut(s) 220, 233, 323
Csp6I GTAC 4 cut(s) 256, 415, 718, 928
CviAII CATG 3 cut(s) 174, 259, 1199
CviQI GTAC 4 cut(s) 256, 415, 718, 928
DdeI CTNAG 3 cut(s) 513, 620, 791
DinI GGCGCC 1 cut(s) 88
DpnI GATC 5 cut(s) 329, 491, 796, 912, 966
DpnII GATC 5 cut(s) 327, 489, 794, 910, 964
DrdI GACNNNNNNGTC 2 cut(s) 230, 320
DseDI GACNNNNNNGTC 2 cut(s) 230, 320
Eco130I CCWWGG 4 cut(s) 157, 173, 478, 1011
Eco47I GGWCC 2 cut(s) 475, 770
Eco57I CTGAAG 2 cut(s) 972, 1075
EcoNI CCTNNNNNAGG 1 cut(s) 587
EcoO109I RGGNCCY 1 cut(s) 770
EcoRII CCWGG 2 cut(s) 396, 880
EcoT14I CCWWGG 4 cut(s) 157, 173, 478, 1011
EcoT22I ATGCAT 1 cut(s) 677
EgeI GGCGCC 1 cut(s) 88
EheI GGCGCC 1 cut(s) 88
ErhI CCWWGG 4 cut(s) 157, 173, 478, 1011
FaeI CATG 3 cut(s) 177, 262, 1202
FaqI GGGAC 2 cut(s) 92, 630
FatI CATG 3 cut(s) 173, 258, 1198
FauI CCCGC 1 cut(s) 493
FauNDI CATATG 1 cut(s) 671
FblI GTMKAC 2 cut(s) 234, 390
Fnu4HI GCNGC 8 cut(s) 12, 90, 93, 362, 441, 498, 1027, 1251
FokI GGATG 2 cut(s) 380, 923
Fsp4HI GCNGC 8 cut(s) 12, 90, 93, 362, 441, 498, 1027, 1251
FspBI CTAG 1 cut(s) 1038
GlaI GCGC 3 cut(s) 88, 450, 496
GluI GCNGC 8 cut(s) 12, 90, 93, 362, 441, 498, 1027, 1251
HaeII RGCGCY 1 cut(s) 90
HaeIII GGCC 7 cut(s) 156, 270, 339, 402, 530, 1080, 1134
HapII CCGG 7 cut(s) 325, 374, 403, 420, 707, 1082, 1254
HgaI GACGC 3 cut(s) 220, 233, 323
HhaI GCGC 3 cut(s) 89, 451, 497
Hin1I GRCGYC 3 cut(s) 87, 225, 315
Hin1II CATG 3 cut(s) 177, 262, 1202
Hin6I GCGC 3 cut(s) 87, 449, 495
HinP1I GCGC 3 cut(s) 87, 449, 495
HincII GTYRAC 1 cut(s) 235
HindII GTYRAC 1 cut(s) 235
HinfI GANTC 7 cut(s) 38, 43, 579, 703, 736, 799, 1102
HpaII CCGG 7 cut(s) 325, 374, 403, 420, 707, 1082, 1254
HphI GGTGA 3 cut(s) 199, 515, 623
Hpy166II GTNNAC 4 cut(s) 235, 391, 904, 1259
Hpy188I TCNGA 9 cut(s) 37, 129, 313, 741, 777, 910, 984, 1094, 1286
Hpy188III TCNNGA 2 cut(s) 325, 555
Hpy8I GTNNAC 4 cut(s) 235, 391, 904, 1259
Hpy99I CGWCG 8 cut(s) 86, 227, 236, 254, 269, 278, 317, 338
HpyAV CCTTC 1 cut(s) 583
HpyCH4III ACNGT 3 cut(s) 239, 436, 1033
HpyCH4IV ACGT 3 cut(s) 321, 333, 429
HpyCH4V TGCA 8 cut(s) 526, 632, 675, 725, 904, 1019, 1075, 1211
HpyF10VI GCNNNNNNNGC 7 cut(s) 370, 446, 536, 545, 1077, 1131, 1208
HpyF3I CTNAG 3 cut(s) 513, 620, 791
HpySE526I ACGT 3 cut(s) 321, 333, 429
Hsp92I GRCGYC 3 cut(s) 87, 225, 315
Hsp92II CATG 3 cut(s) 177, 262, 1202
HspAI GCGC 3 cut(s) 87, 449, 495
KasI GGCGCC 1 cut(s) 86
Kpn2I TCCGGA 1 cut(s) 324
KspI CCGCGG 1 cut(s) 444
Kzo9I GATC 5 cut(s) 327, 489, 794, 910, 964
LmnI GCTCC 1 cut(s) 1180
Lsp1109I GCAGC 3 cut(s) 348, 509, 1237
LweI GCATC 1 cut(s) 457
MaeI CTAG 1 cut(s) 1038
MaeII ACGT 3 cut(s) 321, 333, 429
MaeIII GTNAC 4 cut(s) 430, 862, 950, 1108
MalI GATC 5 cut(s) 329, 491, 796, 912, 966
MboI GATC 5 cut(s) 327, 489, 794, 910, 964
MboII GAAGA 6 cut(s) 38, 286, 745, 905, 908, 1081
MhlI GDGCHC 1 cut(s) 906
MluCI AATT 3 cut(s) 761, 1006, 1190
Mly113I GGCGCC 1 cut(s) 87
MlyI GAGTC 2 cut(s) 52, 793
MmeI TCCRAC 1 cut(s) 336
Mph1103I ATGCAT 1 cut(s) 677
MroI TCCGGA 1 cut(s) 324
MroXI GAANNNNTTC 1 cut(s) 182
MspA1I CMGCKG 2 cut(s) 443, 500
MspI CCGG 7 cut(s) 325, 374, 403, 420, 707, 1082, 1254
MspR9I CCNGG 5 cut(s) 398, 404, 421, 882, 1082
Mva1269I GAATGC 2 cut(s) 843, 1085
MvaI CCWGG 2 cut(s) 398, 882
MvnI CGCG 3 cut(s) 254, 443, 932
MwoI GCNNNNNNNGC 7 cut(s) 370, 446, 536, 545, 1077, 1131, 1208
NarI GGCGCC 1 cut(s) 87
NciI CCSGG 3 cut(s) 404, 421, 1082
NcoI CCATGG 1 cut(s) 173
NdeI CATATG 1 cut(s) 671
NdeII GATC 5 cut(s) 327, 489, 794, 910, 964
NlaIII CATG 3 cut(s) 177, 262, 1202
NlaIV GGNNCC 3 cut(s) 88, 696, 1176
NmeAIII GCCGAG 1 cut(s) 1228
NmuCI GTSAC 3 cut(s) 430, 862, 1108
NsiI ATGCAT 1 cut(s) 677
NspI RCATGY 2 cut(s) 262, 1202
PcsI WCGNNNNNNNCGW 3 cut(s) 231, 270, 273
PctI GAATGC 2 cut(s) 843, 1085
PdmI GAANNNNTTC 1 cut(s) 182
PfeI GAWTC 5 cut(s) 38, 579, 703, 736, 1102
PflFI GACNNNGTC 1 cut(s) 386
PkrI GCNGC 8 cut(s) 13, 91, 94, 363, 442, 499, 1028, 1252
PleI GAGTC 2 cut(s) 51, 793
PluTI GGCGCC 1 cut(s) 90
PpsI GAGTC 2 cut(s) 51, 793
PpuMI RGGWCCY 1 cut(s) 770
Psp5II RGGWCCY 1 cut(s) 770
Psp6I CCWGG 2 cut(s) 396, 880
PspGI CCWGG 2 cut(s) 396, 880
PspN4I GGNNCC 3 cut(s) 88, 696, 1176
PspPI GGNCC 6 cut(s) 154, 400, 475, 770, 1079, 1132
PspPPI RGGWCCY 1 cut(s) 770
PstI CTGCAG 1 cut(s) 1213
PsyI GACNNNGTC 1 cut(s) 386
RsaI GTAC 4 cut(s) 257, 416, 719, 929
RsaNI GTAC 4 cut(s) 256, 415, 718, 928
SacII CCGCGG 1 cut(s) 444
SalI GTCGAC 1 cut(s) 233
SatI GCNGC 8 cut(s) 12, 90, 93, 362, 441, 498, 1027, 1251
Sau3AI GATC 5 cut(s) 327, 489, 794, 910, 964
Sau96I GGNCC 6 cut(s) 154, 400, 475, 770, 1079, 1132
ScaI AGTACT 1 cut(s) 719
SchI GAGTC 2 cut(s) 52, 793
ScrFI CCNGG 5 cut(s) 398, 404, 421, 882, 1082
SduI GDGCHC 1 cut(s) 906
SetI ASST 9 cut(s) 134, 168, 212, 216, 324, 336, 432, 772, 1017
SfaNI GCATC 1 cut(s) 457
SfcI CTRYAG 1 cut(s) 1209
SfoI GGCGCC 1 cut(s) 88
Sfr303I CCGCGG 1 cut(s) 444
SgrBI CCGCGG 1 cut(s) 444
SinI GGWCC 2 cut(s) 475, 770
SmlI CTYRAG 1 cut(s) 917
SmoI CTYRAG 1 cut(s) 917
Sse9I AATT 3 cut(s) 761, 1006, 1190
SsiI CCGC 9 cut(s) 12, 90, 93, 441, 443, 500, 602, 932, 1026
SspDI GGCGCC 1 cut(s) 86
SspI AATATT 1 cut(s) 876
SspMI CTAG 1 cut(s) 1038
StyD4I CCNGG 5 cut(s) 396, 402, 419, 880, 1080
StyI CCWWGG 4 cut(s) 157, 173, 478, 1011
TaaI ACNGT 3 cut(s) 239, 436, 1033
TaiI ACGT 3 cut(s) 324, 336, 432
TaqI TCGA 3 cut(s) 41, 234, 797
TasI AATT 3 cut(s) 761, 1006, 1190
TatI WGTACW 1 cut(s) 717
TauI GCSGC 5 cut(s) 14, 92, 95, 443, 1029
TfiI GAWTC 5 cut(s) 38, 579, 703, 736, 1102
TscAI CASTG 2 cut(s) 550, 1113
TseFI GTSAC 3 cut(s) 430, 862, 1108
TseI GCWGC 3 cut(s) 361, 497, 1250
Tsp45I GTSAC 3 cut(s) 430, 862, 1108
TspDTI ATGAA 2 cut(s) 175, 728
TspGWI ACGGA 1 cut(s) 238
TspRI CASTG 2 cut(s) 550, 1113
Tth111I GACNNNGTC 1 cut(s) 386
VneI GTGCAC 1 cut(s) 902
VpaK11BI GGWCC 2 cut(s) 475, 770
XagI CCTNNNNNAGG 1 cut(s) 587
XapI RAATTY 1 cut(s) 1006
XceI RCATGY 2 cut(s) 262, 1202
XmiI GTMKAC 2 cut(s) 234, 390
XmnI GAANNNNTTC 1 cut(s) 182
XspI CTAG 1 cut(s) 1038
ZrmI AGTACT 1 cut(s) 719
Zsp2I ATGCAT 1 cut(s) 677
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.