AT1G73850

Protein of unknown function (DUF1666)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0008073
Physical Location & Seq
Forward (+)
27766941 .. 27770841
3901 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G73850.1

Sequence Viewer

Length: 1908 bp
ATGCAATGTTATACAAATGAAGAAGCACTAGCAAGTCTCTTCATCAATGTCTCAACATCTTTCTTTCTTCTCCTCATCCTCCTCTACTCCGCCGTCTCCCTCCTAATCAAGCTTCTCCACTTCATCGGCGGCTACCCTCTCCTCCAAAGGAATGAAGATGAATATGATTCCGCGGCATGGTATTCCGAAGAAGAGGAGGAAGAGGAAGAAGAAGGGGAAGAACTCAAGATGTCGTGTAACTCAAGTTACCATGTGATATCTAGAGATCCAAATCCGGATTTGATAGCTGATATAGCAGACGACGGAGAATCTTTAGTGTTTTACAATAATATTTCTCAAGGAGGAGTAAATAATCAACACACGAAGGAGTTCAATAATGTCTATCAAACTCATGAACACGAAGAAGAGGAAGAAGATGATCAAGATTCTAACACTTCTTCTACAGAGGAGCACTTTTCTTCTGCCAATGTTTCTCCTTATCGCAGTGAATCATCAATAGAAGAGGAAGATGATGGCGTTGAAGATCTTCCTGACGATCGTTATGATGATGATGACGAAGATGAAGAGGTAGGAGGAGTGTCACGATACGACGTCGTTGAGGATTTGGTTCGTAAGCAACCAAATACTACTAGTACTCGTGGTCCCTCTCGTTTCCAAAGTGGTTTGGTGTTCAATGACAAAAGTTACAATGCAAGAGAGTTTGTGTCCAATGGAGGAATCAAGAATGATGTCGACGAGATACAGCCATCATCGGGCTTTGACATGAGAGAGATAAAAGCAGAGGAATTAGAGGAAGAAGAAGAAGAAGAGAGAGGACAAATATTCGGAGAGTCATGCACGAATGGGTCAACGTCGAAAAGCTCATCGGAATGGAGAAACTCTGTCAAAACTGACGACCCTTTCTCAACGTCCTCTCGCCGGAGTTGCCCTAAATGGGAATCTTACACCGTCTTCCAAAAGTACGACGAAGAAATGACTTTTCTCACTAGAATCTCTGCTCAAAAGCTTCACGAAGCAGAATCTTTGAAGTCAATTATGGTGGAGCCAAGATCTATCTCGGAGAGGATCGTTCACAAGTTATCATCAAACGGTCACAAGAAAAAACAAAAACAATATCCAGGCAGCAATGGGTCGAGACCAAACCCTTACGTGGAACTAGAATCCGCTTACGTGGCACAGATATGTCTAACATGGGAAGCTCTAAGTTGGAACTACAAAAACTTCGAGAGAAAAAGATCAACCACGCAACGAAGCTTCAACGACGTAGGATGTCCCGCCGGAATCGCCGACCAATTCCGTACGTTTCACATTCTTTTACAAAGATACGTAGAAAATGAACCTTATGAACATGGAAGAAGACCAGAGATTTATGCTCGCATGCGAACTCTTGCTCCTAAATTGCTTCTTGTTCCTGAATACCAAGATTATGAGGAAGAGGAGGAGAAGGAAGACGAGAACGAAGAGGGTTTCAGGTCGAGGATATCGTCAGCTTCGTTCTTGATGATAATGGAGGAATGCATAAGAACATTCATGAATTTTCTCCAAGCGGATAAAGAAAAGCCTTGTCAGAAGATCATCAAAGCCTTTTTTGGGAGAAGTAAACGAGGCCCCGTTGATCCAACCCTTGTACATCTCATGAAGAAAGTCAACACAAAGAAAAAGACGAAGCTGAAAGAGATGCGTCGAGGAGGGAAATACATGAGGAAGAAGAAGATGAGTATAGAGGAGGAAATGGAGATATTGATGGGATTAATAGATTTGAAAGTGGTTTCAAGAGTGTTAAGGATGAATGAAATGAACGAAGAGAATTTGCATTGGTGTGAAGAGAAGATGAGCAAAGTTAAAATTATTCAAGGTGGAAAGGTGCTTCAAAGAGATTCCACTCCACTTTTCTTTCCTCCACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1666

Protein Analysis

635

Amino Acids

73.59

Weight (kDa)

4.86

Isoelectric Point (pI)

65.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1666 PF07891 385 - 633 2.3e-85 Protein of unknown function (DUF1666)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 594
AccI GTMKAC 1 cut(s) 732
AccII CGCG 1 cut(s) 173
AccIII TCCGGA 1 cut(s) 274
AciI CCGC 7 cut(s) 90, 129, 171, 173, 1164, 1275, 1547
AclWI GGATC 3 cut(s) 260, 1073, 1610
AcsI RAATTY 2 cut(s) 1534, 1807
AcyI GRCGYC 1 cut(s) 591
AfaI GTAC 4 cut(s) 634, 962, 1300, 1629
AfiI CCNNNNNNNGG 6 cut(s) 177, 752, 918, 934, 1150, 1590
AgsI TTSAA 9 cut(s) 373, 521, 673, 1027, 1258, 1762, 1773, 1853, 1871
AhlI ACTAGT 1 cut(s) 629
AjnI CCWGG 1 cut(s) 1117
AloI GAACNNNNNNTCC 2 cut(s) 1375, 1407
AluBI AGCT 8 cut(s) 112, 287, 861, 1006, 1199, 1254, 1490, 1669
AluI AGCT 8 cut(s) 112, 287, 861, 1006, 1199, 1254, 1490, 1669
Alw21I GWGCWC 1 cut(s) 453
Alw26I GTCTC 4 cut(s) 41, 55, 100, 1129
AlwI GGATC 3 cut(s) 260, 1073, 1610
Aor13HI TCCGGA 1 cut(s) 274
AoxI GGCC 1 cut(s) 1606
ApeKI GCWGC 1 cut(s) 1122
ApoI RAATTY 2 cut(s) 1534, 1807
AseI ATTAAT 1 cut(s) 1751
Asp700I GAANNNNTTC 2 cut(s) 368, 525
AspS9I GGNCC 2 cut(s) 641, 1607
AvaII GGWCC 1 cut(s) 641
BauI CACGAG 1 cut(s) 636
BbsI GAAGAC 3 cut(s) 943, 1363, 1455
Bbv12I GWGCWC 1 cut(s) 453
BbvI GCAGC 1 cut(s) 1134
BccI CCATC 3 cut(s) 506, 754, 1738
BceAI ACGGC 1 cut(s) 77
BciT130I CCWGG 1 cut(s) 1119
BclI TGATCA 1 cut(s) 418
BcoDI GTCTC 4 cut(s) 41, 55, 100, 1129
BcuI ACTAGT 1 cut(s) 629
BfaI CTAG 5 cut(s) 29, 261, 630, 987, 1157
BfmI CTRYAG 1 cut(s) 441
BglII AGATCT 2 cut(s) 523, 1049
BisI GCNGC 3 cut(s) 130, 174, 1123
BlsI GCNGC 3 cut(s) 131, 175, 1124
BmcAI AGTACT 1 cut(s) 634
Bme1390I CCNGG 1 cut(s) 1119
Bme18I GGWCC 1 cut(s) 641
BmgT120I GGNCC 2 cut(s) 641, 1607
BmiI GGNNCC 3 cut(s) 643, 1044, 1609
BmrFI CCNGG 1 cut(s) 1119
BmsI GCATC 1 cut(s) 1668
BpiI GAAGAC 3 cut(s) 943, 1363, 1455
BpuEI CTTGAG 3 cut(s) 209, 226, 321
BsaAI YACGTR 3 cut(s) 1150, 1171, 1327
BsaBI GATNNNNATC 1 cut(s) 270
BsaHI GRCGYC 1 cut(s) 591
BsaI GGTCTC 1 cut(s) 1129
BsaJI CCNNGG 1 cut(s) 171
BsaWI WCCGGW 1 cut(s) 274
BsaXI ACNNNNNCTCC 2 cut(s) 1375, 1405
Bsc4I CCNNNNNNNGG 6 cut(s) 177, 752, 918, 934, 1150, 1590
Bse3DI GCAATG 2 cut(s) 11, 1132
Bse8I GATNNNNATC 1 cut(s) 270
BseAI TCCGGA 1 cut(s) 274
BseBI CCWGG 1 cut(s) 1119
BseDI CCNNGG 1 cut(s) 171
BseGI GGATG 3 cut(s) 75, 1274, 1791
BseJI GATNNNNATC 1 cut(s) 270
BseLI CCNNNNNNNGG 6 cut(s) 177, 752, 918, 934, 1150, 1590
BseMI GCAATG 2 cut(s) 11, 1132
BseXI GCAGC 1 cut(s) 1134
Bsh1236I CGCG 1 cut(s) 173
Bsh1285I CGRYCG 1 cut(s) 538
BshFI GGCC 1 cut(s) 1608
BsiEI CGRYCG 1 cut(s) 538
BsiHKAI GWGCWC 1 cut(s) 453
BsiSI CCGG 3 cut(s) 275, 919, 1278
BsiWI CGTACG 1 cut(s) 1298
BslFI GGGAC 2 cut(s) 627, 1257
BslI CCNNNNNNNGG 6 cut(s) 177, 752, 918, 934, 1150, 1590
BsmAI GTCTC 4 cut(s) 41, 55, 100, 1129
BsmBI CGTCTC 1 cut(s) 100
BsmFI GGGAC 2 cut(s) 627, 1257
BsmI GAATGC 1 cut(s) 1520
BsnI GGCC 1 cut(s) 1608
Bso31I GGTCTC 1 cut(s) 1129
Bsp1286I GDGCHC 1 cut(s) 453
Bsp13I TCCGGA 1 cut(s) 274
Bsp1407I TGTACA 1 cut(s) 1627
Bsp143I GATC 9 cut(s) 265, 418, 523, 535, 1049, 1065, 1235, 1572, 1615
BspACI CCGC 7 cut(s) 90, 129, 171, 173, 1164, 1275, 1547
BspANI GGCC 1 cut(s) 1608
BspEI TCCGGA 1 cut(s) 274
BspFNI CGCG 1 cut(s) 173
BspHI TCATGA 3 cut(s) 391, 1530, 1635
BspLI GGNNCC 3 cut(s) 643, 1044, 1609
BspPI GGATC 3 cut(s) 260, 1073, 1610
BspTNI GGTCTC 1 cut(s) 1129
BsrDI GCAATG 2 cut(s) 11, 1132
BsrGI TGTACA 1 cut(s) 1627
BssECI CCNNGG 1 cut(s) 171
BssMI GATC 9 cut(s) 265, 418, 523, 535, 1049, 1065, 1235, 1572, 1615
BssNI GRCGYC 1 cut(s) 591
BssSI CACGAG 1 cut(s) 636
Bst2BI CACGAG 1 cut(s) 636
Bst2UI CCWGG 1 cut(s) 1119
Bst4CI ACNGT 2 cut(s) 949, 1091
BstACI GRCGYC 1 cut(s) 591
BstAUI TGTACA 1 cut(s) 1627
BstBAI YACGTR 3 cut(s) 1150, 1171, 1327
BstC8I GCNNGC 2 cut(s) 1375, 1379
BstDEI CTNAG 1 cut(s) 1202
BstDSI CCRYGG 1 cut(s) 171
BstF5I GGATG 3 cut(s) 75, 1274, 1791
BstFNI CGCG 1 cut(s) 173
BstKTI GATC 9 cut(s) 268, 421, 526, 538, 1052, 1068, 1238, 1575, 1618
BstMAI GTCTC 4 cut(s) 41, 55, 100, 1129
BstMBI GATC 9 cut(s) 265, 418, 523, 535, 1049, 1065, 1235, 1572, 1615
BstMCI CGRYCG 1 cut(s) 538
BstMWI GCNNNNNNNGC 4 cut(s) 293, 924, 1172, 1283
BstNI CCWGG 1 cut(s) 1119
BstNSI RCATGY 1 cut(s) 1381
BstSCI CCNGG 1 cut(s) 1117
BstSFI CTRYAG 1 cut(s) 441
BstSNI TACGTA 1 cut(s) 1327
BstUI CGCG 1 cut(s) 173
BstV1I GCAGC 1 cut(s) 1134
BstV2I GAAGAC 3 cut(s) 943, 1363, 1455
BstX2I RGATCY 3 cut(s) 265, 523, 1049
BstYI RGATCY 3 cut(s) 265, 523, 1049
BsuRI GGCC 1 cut(s) 1608
BtgI CCRYGG 1 cut(s) 171
BtsCI GGATG 3 cut(s) 75, 1274, 1791
BtsI GCAGTG 1 cut(s) 490
BtsIMutI CAGTG 1 cut(s) 490
Cac8I GCNNGC 2 cut(s) 1375, 1379
CciI TCATGA 3 cut(s) 391, 1530, 1635
Cfr13I GGNCC 2 cut(s) 641, 1607
Cfr42I CCGCGG 1 cut(s) 174
CseI GACGC 1 cut(s) 1670
Csp6I GTAC 4 cut(s) 633, 961, 1299, 1628
CspCI CAANNNNNGTGG 2 cut(s) 1020, 1055
CviQI GTAC 4 cut(s) 633, 961, 1299, 1628
DdeI CTNAG 1 cut(s) 1202
DpnI GATC 9 cut(s) 267, 420, 525, 537, 1051, 1067, 1237, 1574, 1617
DpnII GATC 9 cut(s) 265, 418, 523, 535, 1049, 1065, 1235, 1572, 1615
EciI GGCGGA 1 cut(s) 79
Eco105I TACGTA 1 cut(s) 1327
Eco31I GGTCTC 1 cut(s) 1129
Eco32I GATATC 2 cut(s) 258, 1482
Eco47I GGWCC 1 cut(s) 641
EcoO109I RGGNCCY 1 cut(s) 1607
EcoRII CCWGG 1 cut(s) 1117
EcoRV GATATC 2 cut(s) 258, 1482
EcoT22I ATGCAT 1 cut(s) 1520
Esp3I CGTCTC 1 cut(s) 100
FaqI GGGAC 2 cut(s) 627, 1257
FauI CCCGC 1 cut(s) 1282
FbaI TGATCA 1 cut(s) 418
FblI GTMKAC 1 cut(s) 732
Fnu4HI GCNGC 3 cut(s) 130, 174, 1123
FokI GGATG 3 cut(s) 62, 1281, 1798
Fsp4HI GCNGC 3 cut(s) 130, 174, 1123
FspBI CTAG 5 cut(s) 29, 261, 630, 987, 1157
GluI GCNGC 3 cut(s) 130, 174, 1123
HaeIII GGCC 1 cut(s) 1608
HapII CCGG 3 cut(s) 275, 919, 1278
HgaI GACGC 1 cut(s) 1670
Hin1I GRCGYC 1 cut(s) 591
HincII GTYRAC 3 cut(s) 733, 849, 1648
HindII GTYRAC 3 cut(s) 733, 849, 1648
HindIII AAGCTT 3 cut(s) 110, 1004, 1252
HpaII CCGG 3 cut(s) 275, 919, 1278
Hpy166II GTNNAC 5 cut(s) 733, 849, 1072, 1601, 1648
Hpy188I TCNGA 5 cut(s) 187, 827, 868, 1060, 1569
Hpy8I GTNNAC 5 cut(s) 733, 849, 1072, 1601, 1648
Hpy99I CGWCG 8 cut(s) 305, 593, 596, 737, 856, 968, 1265, 1686
HpyAV CCTTC 3 cut(s) 206, 358, 1438
HpyCH4III ACNGT 2 cut(s) 949, 1091
HpyCH4IV ACGT 8 cut(s) 591, 851, 908, 1149, 1170, 1263, 1301, 1326
HpyCH4V TGCA 5 cut(s) 4, 692, 837, 1518, 1813
HpyF10VI GCNNNNNNNGC 4 cut(s) 293, 924, 1172, 1283
HpyF3I CTNAG 1 cut(s) 1202
HpySE526I ACGT 8 cut(s) 591, 851, 908, 1149, 1170, 1263, 1301, 1326
Hsp92I GRCGYC 1 cut(s) 591
Kpn2I TCCGGA 1 cut(s) 274
Ksp22I TGATCA 1 cut(s) 418
KspI CCGCGG 1 cut(s) 174
Kzo9I GATC 9 cut(s) 265, 418, 523, 535, 1049, 1065, 1235, 1572, 1615
LmnI GCTCC 3 cut(s) 448, 1042, 1396
LpnPI CCDG 9 cut(s) 288, 543, 932, 1104, 1131, 1291, 1374, 1425, 1456
Lsp1109I GCAGC 1 cut(s) 1134
LweI GCATC 1 cut(s) 1668
MaeI CTAG 5 cut(s) 29, 261, 630, 987, 1157
MaeII ACGT 8 cut(s) 591, 851, 908, 1149, 1170, 1263, 1301, 1326
MaeIII GTNAC 5 cut(s) 236, 245, 579, 683, 1091
MalI GATC 9 cut(s) 267, 420, 525, 537, 1051, 1067, 1237, 1574, 1617
MboI GATC 9 cut(s) 265, 418, 523, 535, 1049, 1065, 1235, 1572, 1615
MflI RGATCY 3 cut(s) 265, 523, 1049
MhlI GDGCHC 1 cut(s) 453
MluCI AATT 7 cut(s) 785, 1032, 1292, 1397, 1534, 1807, 1845
MlyI GAGTC 1 cut(s) 839
MmeI TCCRAC 2 cut(s) 1187, 1643
Mph1103I ATGCAT 1 cut(s) 1520
MroI TCCGGA 1 cut(s) 274
MroXI GAANNNNTTC 2 cut(s) 368, 525
MseI TTAA 3 cut(s) 1751, 1781, 1842
MslI CAYNNNNRTG 3 cut(s) 868, 1180, 1818
MspA1I CMGCKG 1 cut(s) 173
MspI CCGG 3 cut(s) 275, 919, 1278
MspR9I CCNGG 1 cut(s) 1119
Mva1269I GAATGC 1 cut(s) 1520
MvaI CCWGG 1 cut(s) 1119
MvnI CGCG 1 cut(s) 173
MwoI GCNNNNNNNGC 4 cut(s) 293, 924, 1172, 1283
NdeII GATC 9 cut(s) 265, 418, 523, 535, 1049, 1065, 1235, 1572, 1615
NlaIV GGNNCC 3 cut(s) 643, 1044, 1609
NmuCI GTSAC 2 cut(s) 579, 1091
NsiI ATGCAT 1 cut(s) 1520
NspI RCATGY 1 cut(s) 1381
PaeI GCATGC 1 cut(s) 1381
PagI TCATGA 3 cut(s) 391, 1530, 1635
PcsI WCGNNNNNNNCGW 2 cut(s) 1481, 1490
PctI GAATGC 1 cut(s) 1520
PdmI GAANNNNTTC 2 cut(s) 368, 525
Pfl23II CGTACG 1 cut(s) 1298
PkrI GCNGC 3 cut(s) 131, 175, 1124
Ple19I CGATCG 1 cut(s) 538
PleI GAGTC 1 cut(s) 838
PpsI GAGTC 1 cut(s) 838
Ppu21I YACGTR 3 cut(s) 1150, 1171, 1327
PshBI ATTAAT 1 cut(s) 1751
Psp6I CCWGG 1 cut(s) 1117
PspGI CCWGG 1 cut(s) 1117
PspLI CGTACG 1 cut(s) 1298
PspN4I GGNNCC 3 cut(s) 643, 1044, 1609
PspPI GGNCC 2 cut(s) 641, 1607
PsuI RGATCY 3 cut(s) 265, 523, 1049
PvuI CGATCG 1 cut(s) 538
RsaI GTAC 4 cut(s) 634, 962, 1300, 1629
RsaNI GTAC 4 cut(s) 633, 961, 1299, 1628
RseI CAYNNNNRTG 3 cut(s) 868, 1180, 1818
SacII CCGCGG 1 cut(s) 174
SalI GTCGAC 1 cut(s) 731
SaqAI TTAA 3 cut(s) 1751, 1781, 1842
SatI GCNGC 3 cut(s) 130, 174, 1123
Sau3AI GATC 9 cut(s) 265, 418, 523, 535, 1049, 1065, 1235, 1572, 1615
Sau96I GGNCC 2 cut(s) 641, 1607
ScaI AGTACT 1 cut(s) 634
SchI GAGTC 1 cut(s) 839
ScrFI CCNGG 1 cut(s) 1119
SduI GDGCHC 1 cut(s) 453
SfaNI GCATC 1 cut(s) 1668
SfcI CTRYAG 1 cut(s) 441
Sfr303I CCGCGG 1 cut(s) 174
SgrBI CCGCGG 1 cut(s) 174
SinI GGWCC 1 cut(s) 641
SmiMI CAYNNNNRTG 3 cut(s) 868, 1180, 1818
SmlI CTYRAG 3 cut(s) 224, 241, 336
SmoI CTYRAG 3 cut(s) 224, 241, 336
SnaBI TACGTA 1 cut(s) 1327
SpeI ACTAGT 1 cut(s) 629
SphI GCATGC 1 cut(s) 1381
Sse9I AATT 7 cut(s) 785, 1032, 1292, 1397, 1534, 1807, 1845
SsiI CCGC 7 cut(s) 90, 129, 171, 173, 1164, 1275, 1547
SspI AATATT 2 cut(s) 331, 822
SspMI CTAG 5 cut(s) 29, 261, 630, 987, 1157
StyD4I CCNGG 1 cut(s) 1117
TaaI ACNGT 2 cut(s) 949, 1091
TaiI ACGT 8 cut(s) 594, 854, 911, 1152, 1173, 1266, 1304, 1329
TaqI TCGA 6 cut(s) 732, 854, 1133, 1224, 1475, 1684
TasI AATT 7 cut(s) 785, 1032, 1292, 1397, 1534, 1807, 1845
TatI WGTACW 2 cut(s) 632, 1627
TauI GCSGC 2 cut(s) 132, 176
Tru1I TTAA 3 cut(s) 1751, 1781, 1842
Tru9I TTAA 3 cut(s) 1751, 1781, 1842
TscAI CASTG 1 cut(s) 490
TseFI GTSAC 2 cut(s) 579, 1091
TseI GCWGC 1 cut(s) 1122
Tsp45I GTSAC 2 cut(s) 579, 1091
TspGWI ACGGA 2 cut(s) 318, 1286
TspRI CASTG 1 cut(s) 490
VpaK11BI GGWCC 1 cut(s) 641
VspI ATTAAT 1 cut(s) 1751
XapI RAATTY 2 cut(s) 1534, 1807
XbaI TCTAGA 1 cut(s) 260
XceI RCATGY 1 cut(s) 1381
XmiI GTMKAC 1 cut(s) 732
XmnI GAANNNNTTC 2 cut(s) 368, 525
XspI CTAG 5 cut(s) 29, 261, 630, 987, 1157
ZraI GACGTC 1 cut(s) 592
ZrmI AGTACT 1 cut(s) 634
Zsp2I ATGCAT 1 cut(s) 1520
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.