FvH4_5g12100

Protein of unknown function (DUF1666)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0008073
Physical Location & Seq
Forward (+)
6828322 .. 6831687
3366 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g12100.t3

Sequence Viewer

Length: 1767 bp
ATGCCATGTTCAACAGAGGAAGCACTTGCTAGGCTCTTCTACAATACTTCATCCTCCGTCTACCTCCTCCTTCTCTTCCTCTATGTCGCATTCATTGTTCTTGCCAAGTTCTTCTGCTTCATTGGCGGCAACCCAATATTCCCAAGATACCAGAATGGATACGAAAACTATGTGTTTTCCGATGAAGAAGAGGAGGAAGAAGAAGAGTTAGAAGAAAGGCACAGCTATCATGTTCATGGTCATCATCAGTACTACACTGAAAACGATCATCTGGTGGCCGACATCATTCATGGAGGTGAAGCACTAAAGTTTCTGCCTGATGACAACCTTCAGAGGAATCGGTCAAGTTTTTCTGATCAAGAGTTCATTAGCGCCGGGGAGAGCCTAATTGAAGAAGACCATGATATGTCACAGGGTTCAGAATCAGAACAAGATCATAGTGCAAATGAAATCCCGGTACGAGATACTGCTTCTGTTCGCAATTCTGTCTCGAATGATCGAGTCTGGACCACTTCTCCGATCACCATAGACCTACACAGAGGTGACTTGAGGAGAAAGGACAAAAAATGTGACGATAATCTAGAGGATTCTGATCAGATTACCGAAAACAATACAGAGACACAATTATCTGGGGATGAAAATTTTCTCGTATTTAGGCCGCCCCAGTTGGAGACCAAGAAATTAATCCGAGTTCAAGAAAACAAAGAGCATGAAATTTTTGGTGACTCAACAGTGGGATCAACTTCTAAGAGCTCTTCTGAATGGAGAAGCTCAATCAATTGTTCTGCGACCGAAGATCCATTTTCTTCCTCGTCACGTAGAAGCTGCCCCAAGTGGGAGTCTTACACTGTCTTTCAGAAATATGATGAAGAAATGATGTACCTCGACAGAATTAGTGCCCAAAAGCTCCATGAAACAGAATCACTTAGATCTATTCAAGTTTGCCCAAGATCGATTTCAGAGAGGATTGTTCATCGAATCTCATTAGCAACTAAAAGGCCATCTGACGTACGTCACAACCCTTACCACGAACTAGAGGCTGCTTATGTTGCTCAGATTTGCTTAACGTGGGAAGCACTTAACTGGAACTACAAGAAATTCGAGCAAAAAAGGGCCTCGTCGCATCACGATTTTGACCCTGGCTGTCCTGGCAAGATTGCGCAGCAGTTTCAGCAGTTCCAAGTTCTTCTGCAGAGATATGTAGAGAATGAGCCCTACGAGCAGGGCCGGAGACCTGAGATTTACGCTAGGATGAGGCTTTTGGCACCAAAGCTACTTCAAGTTCCAGAATATCGAGCTTCAGAAGATGATGATGATCATGAAAAGGAAGAAGGTTTTGGCTCGAGAATCTCCTCAGCAGCGTTTCTAATGGTAATGGAAGATGGAATCCGAACGTTCATGGATTTTCTTAAGGCTGATAAAGAGAAACCCTGCCAAAAGTTATCAGCCATGTTTAGGAGAAAACGAAGAAGTTCAGTTGATCCAACTCTTTTAAATCTCATGAAAAAAGTTAACCAAAAGAAGAAGATGAAGATCAAAGACCTTCGCCGTTCGCACAAGTGCTTGAGAAAGAAAAGCTTGAAGGTGGAGGAGGAGATGGAGATATTGCTAGGCCTAATTGACCTAAAACTGGTCTCTAGGGTTTTGAGAATGAGTGACTTGAGTGCAGAACAACTGCATTGGTGTGAGGAGAAGATGAGCAAAGTGAGAGTCCTGGATGGGAAATACCAGAGAGATTCCACACCACTTTTCTTCCCAGCACAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1666

Protein Analysis

589

Amino Acids

68.62

Weight (kDa)

5.71

Isoelectric Point (pI)

60.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1666 PF07891 208 - 451 3.3e-93 Protein of unknown function (DUF1666)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1161
AccB1I GGYRCC 1 cut(s) 1264
AccI GTMKAC 1 cut(s) 60
AciI CCGC 2 cut(s) 126, 659
AclI AACGTT 1 cut(s) 1394
AclWI GGATC 3 cut(s) 745, 791, 1475
AcoI YGGCCR 1 cut(s) 276
AcsI RAATTY 3 cut(s) 640, 714, 1097
AcuI CTGAAG 2 cut(s) 314, 1284
AfaI GTAC 4 cut(s) 251, 459, 881, 1011
AfiI CCNNNNNNNGG 3 cut(s) 835, 1455, 1630
AflII CTTAAG 1 cut(s) 1409
AgsI TTSAA 6 cut(s) 12, 392, 695, 938, 1280, 1582
AjnI CCWGG 3 cut(s) 1138, 1147, 1713
AjuI GAANNNNNNNTTGG 4 cut(s) 940, 972, 1320, 1352
AleI CACNNNNGTG 1 cut(s) 540
AluBI AGCT 8 cut(s) 225, 753, 771, 825, 907, 1273, 1298, 1578
AluI AGCT 8 cut(s) 225, 753, 771, 825, 907, 1273, 1298, 1578
Alw21I GWGCWC 1 cut(s) 755
Alw26I GTCTC 5 cut(s) 493, 611, 665, 1225, 1639
AlwI GGATC 3 cut(s) 745, 791, 1475
Ama87I CYCGRG 1 cut(s) 1342
AoxI GGCC 6 cut(s) 276, 656, 998, 1113, 1225, 1612
ApeKI GCWGC 4 cut(s) 825, 1040, 1162, 1358
ApoI RAATTY 3 cut(s) 640, 714, 1097
ArsI GACNNNNNNTTYG 2 cut(s) 1695, 1727
AseI ATTAAT 1 cut(s) 683
Asp700I GAANNNNTTC 2 cut(s) 642, 1471
AspLEI GCGC 2 cut(s) 374, 1162
AspS9I GGNCC 3 cut(s) 507, 1113, 1225
AsuC2I CCSGG 2 cut(s) 376, 455
AsuHPI GGTGA 4 cut(s) 308, 514, 554, 734
AvaI CYCGRG 1 cut(s) 1342
AvaII GGWCC 1 cut(s) 507
BaeGI GKGCMC 1 cut(s) 901
BanI GGYRCC 1 cut(s) 1264
BanII GRGCYC 2 cut(s) 755, 1215
BbsI GAAGAC 1 cut(s) 402
Bbv12I GWGCWC 1 cut(s) 755
BbvCI CCTCAGC 1 cut(s) 1354
BbvI GCAGC 4 cut(s) 812, 1027, 1174, 1370
BccI CCATC 4 cut(s) 1009, 1376, 1591, 1712
BceAI ACGGC 1 cut(s) 1533
BciT130I CCWGG 3 cut(s) 1140, 1149, 1715
BciVI GTATCC 1 cut(s) 152
BclI TGATCA 3 cut(s) 355, 592, 1315
BcnI CCSGG 2 cut(s) 376, 455
BcoDI GTCTC 5 cut(s) 493, 611, 665, 1225, 1639
BfaI CTAG 6 cut(s) 30, 581, 1034, 1248, 1610, 1638
BfmI CTRYAG 1 cut(s) 1190
BfoI RGCGCY 1 cut(s) 375
BfrI CTTAAG 1 cut(s) 1409
BfuI GTATCC 1 cut(s) 152
BglII AGATCT 1 cut(s) 929
BisI GCNGC 6 cut(s) 127, 659, 826, 1041, 1163, 1359
BlsI GCNGC 6 cut(s) 128, 660, 827, 1042, 1164, 1360
BmcAI AGTACT 1 cut(s) 251
Bme1390I CCNGG 5 cut(s) 376, 455, 1140, 1149, 1715
Bme18I GGWCC 1 cut(s) 507
BmeT110I CYCGRG 1 cut(s) 1342
BmgT120I GGNCC 3 cut(s) 507, 1113, 1225
BmiI GGNNCC 1 cut(s) 1266
BmrFI CCNGG 5 cut(s) 376, 455, 1140, 1149, 1715
BmrI ACTGGG 1 cut(s) 658
BmsI GCATC 1 cut(s) 1132
BmuI ACTGGG 1 cut(s) 658
BoxI GACNNNNGTC 1 cut(s) 1011
BpiI GAAGAC 1 cut(s) 402
Bpu10I CCTNAGC 1 cut(s) 1354
BpuEI CTTGAG 3 cut(s) 568, 1585, 1681
BpuMI CCSGG 2 cut(s) 376, 455
Bsa29I ATCGAT 1 cut(s) 953
BsaAI YACGTR 1 cut(s) 818
BsaBI GATNNNNATC 3 cut(s) 591, 1314, 1532
BsaI GGTCTC 3 cut(s) 665, 1225, 1639
BsaJI CCNNGG 2 cut(s) 375, 1138
BsaXI ACNNNNNCTCC 2 cut(s) 499, 529
Bsc4I CCNNNNNNNGG 3 cut(s) 835, 1455, 1630
Bse1I ACTGG 3 cut(s) 664, 1088, 1635
Bse8I GATNNNNATC 3 cut(s) 591, 1314, 1532
BseBI CCWGG 3 cut(s) 1140, 1149, 1715
BseCI ATCGAT 1 cut(s) 953
BseDI CCNNGG 2 cut(s) 375, 1138
BseGI GGATG 4 cut(s) 50, 640, 1257, 1723
BseJI GATNNNNATC 3 cut(s) 591, 1314, 1532
BseLI CCNNNNNNNGG 3 cut(s) 835, 1455, 1630
BseMII CTCAG 3 cut(s) 1067, 1227, 1368
BseNI ACTGG 3 cut(s) 664, 1088, 1635
BseRI GAGGAG 7 cut(s) 56, 206, 565, 1342, 1604, 1607, 1703
BseSI GKGCMC 1 cut(s) 901
BseXI GCAGC 4 cut(s) 812, 1027, 1174, 1370
BseYI CCCAGC 1 cut(s) 1756
BsgI GTGCAG 1 cut(s) 1686
Bsh1285I CGRYCG 1 cut(s) 792
BshFI GGCC 6 cut(s) 278, 658, 1000, 1115, 1227, 1614
BshNI GGYRCC 1 cut(s) 1264
BshVI ATCGAT 1 cut(s) 953
BsiEI CGRYCG 1 cut(s) 792
BsiHKAI GWGCWC 1 cut(s) 755
BsiHKCI CYCGRG 1 cut(s) 1342
BsiSI CCGG 3 cut(s) 375, 455, 1228
BsiWI CGTACG 1 cut(s) 1009
BslI CCNNNNNNNGG 3 cut(s) 835, 1455, 1630
BsmAI GTCTC 5 cut(s) 493, 611, 665, 1225, 1639
BsmI GAATGC 1 cut(s) 89
BsnI GGCC 6 cut(s) 278, 658, 1000, 1115, 1227, 1614
Bso31I GGTCTC 3 cut(s) 665, 1225, 1639
BsoBI CYCGRG 1 cut(s) 1342
Bsp1286I GDGCHC 3 cut(s) 755, 901, 1215
BspACI CCGC 2 cut(s) 126, 659
BspANI GGCC 6 cut(s) 278, 658, 1000, 1115, 1227, 1614
BspCNI CTCAG 3 cut(s) 1066, 1228, 1367
BspDI ATCGAT 1 cut(s) 953
BspHI TCATGA 2 cut(s) 1318, 1500
BspLI GGNNCC 1 cut(s) 1266
BspMAI CTGCAG 1 cut(s) 1194
BspPI GGATC 3 cut(s) 745, 791, 1475
BspQI GCTCTTC 2 cut(s) 41, 760
BspT107I GGYRCC 1 cut(s) 1264
BspTI CTTAAG 1 cut(s) 1409
BspTNI GGTCTC 3 cut(s) 665, 1225, 1639
BsrI ACTGG 3 cut(s) 664, 1088, 1635
BssECI CCNNGG 2 cut(s) 375, 1138
Bst2UI CCWGG 3 cut(s) 1140, 1149, 1715
Bst4CI ACNGT 3 cut(s) 733, 850, 1764
Bst6I CTCTTC 5 cut(s) 41, 80, 183, 198, 760
BstAFI CTTAAG 1 cut(s) 1409
BstBAI YACGTR 1 cut(s) 818
BstDEI CTNAG 5 cut(s) 747, 926, 1053, 1236, 1354
BstF5I GGATG 4 cut(s) 50, 640, 1257, 1723
BstH2I RGCGCY 1 cut(s) 375
BstHHI GCGC 2 cut(s) 374, 1162
BstMAI GTCTC 5 cut(s) 493, 611, 665, 1225, 1639
BstMCI CGRYCG 1 cut(s) 792
BstMWI GCNNNNNNNGC 5 cut(s) 123, 1049, 1149, 1171, 1219
BstNI CCWGG 3 cut(s) 1140, 1149, 1715
BstPAI GACNNNNGTC 1 cut(s) 1011
BstSCI CCNGG 5 cut(s) 374, 453, 1138, 1147, 1713
BstSFI CTRYAG 1 cut(s) 1190
BstSLI GKGCMC 1 cut(s) 901
BstV1I GCAGC 4 cut(s) 812, 1027, 1174, 1370
BstV2I GAAGAC 1 cut(s) 402
BstX2I RGATCY 2 cut(s) 796, 929
BstYI RGATCY 2 cut(s) 796, 929
Bsu15I ATCGAT 1 cut(s) 953
BsuI GTATCC 1 cut(s) 152
BsuRI GGCC 6 cut(s) 278, 658, 1000, 1115, 1227, 1614
BsuTUI ATCGAT 1 cut(s) 953
BtsCI GGATG 4 cut(s) 50, 640, 1257, 1723
BtsIMutI CAGTG 3 cut(s) 255, 738, 846
CciI TCATGA 2 cut(s) 1318, 1500
CfoI GCGC 2 cut(s) 374, 1162
Cfr13I GGNCC 3 cut(s) 507, 1113, 1225
ClaI ATCGAT 1 cut(s) 953
Csp6I GTAC 4 cut(s) 250, 458, 880, 1010
CviQI GTAC 4 cut(s) 250, 458, 880, 1010
DdeI CTNAG 5 cut(s) 747, 926, 1053, 1236, 1354
DraI TTTAAA 1 cut(s) 1494
EaeI YGGCCR 1 cut(s) 276
Eam1104I CTCTTC 5 cut(s) 41, 80, 183, 198, 760
EarI CTCTTC 5 cut(s) 41, 80, 183, 198, 760
Ecl136II GAGCTC 1 cut(s) 753
Eco147I AGGCCT 1 cut(s) 1614
Eco24I GRGCYC 2 cut(s) 755, 1215
Eco31I GGTCTC 3 cut(s) 665, 1225, 1639
Eco47I GGWCC 1 cut(s) 507
Eco53kI GAGCTC 1 cut(s) 753
Eco57I CTGAAG 2 cut(s) 314, 1284
Eco88I CYCGRG 1 cut(s) 1342
EcoICRI GAGCTC 1 cut(s) 753
EcoO109I RGGNCCY 1 cut(s) 1113
EcoRII CCWGG 3 cut(s) 1138, 1147, 1713
EcoT38I GRGCYC 2 cut(s) 755, 1215
FalI AAGNNNNNCTT 2 cut(s) 1562, 1594
FbaI TGATCA 3 cut(s) 355, 592, 1315
FblI GTMKAC 1 cut(s) 60
Fnu4HI GCNGC 6 cut(s) 127, 659, 826, 1041, 1163, 1359
FokI GGATG 4 cut(s) 37, 647, 1264, 1730
FriOI GRGCYC 2 cut(s) 755, 1215
Fsp4HI GCNGC 6 cut(s) 127, 659, 826, 1041, 1163, 1359
FspBI CTAG 6 cut(s) 30, 581, 1034, 1248, 1610, 1638
FspI TGCGCA 1 cut(s) 1161
GlaI GCGC 2 cut(s) 373, 1161
GluI GCNGC 6 cut(s) 127, 659, 826, 1041, 1163, 1359
GsaI CCCAGC 1 cut(s) 1760
HaeII RGCGCY 1 cut(s) 375
HaeIII GGCC 6 cut(s) 278, 658, 1000, 1115, 1227, 1614
HapII CCGG 3 cut(s) 375, 455, 1228
HhaI GCGC 2 cut(s) 374, 1162
Hin6I GCGC 2 cut(s) 372, 1160
HinP1I GCGC 2 cut(s) 372, 1160
HincII GTYRAC 1 cut(s) 1513
HindII GTYRAC 1 cut(s) 1513
HindIII AAGCTT 1 cut(s) 1576
HpaI GTTAAC 1 cut(s) 1513
HpaII CCGG 3 cut(s) 375, 455, 1228
HphI GGTGA 4 cut(s) 308, 514, 554, 734
Hpy166II GTNNAC 2 cut(s) 61, 1513
Hpy8I GTNNAC 2 cut(s) 61, 1513
Hpy99I CGWCG 1 cut(s) 1123
HpyAV CCTTC 5 cut(s) 80, 338, 1325, 1553, 1576
HpyCH4III ACNGT 3 cut(s) 733, 850, 1764
HpyCH4IV ACGT 5 cut(s) 817, 1008, 1012, 1067, 1394
HpyCH4V TGCA 4 cut(s) 443, 1192, 1667, 1678
HpyF10VI GCNNNNNNNGC 5 cut(s) 123, 1049, 1149, 1171, 1219
HpyF3I CTNAG 5 cut(s) 747, 926, 1053, 1236, 1354
HpySE526I ACGT 5 cut(s) 817, 1008, 1012, 1067, 1394
HspAI GCGC 2 cut(s) 372, 1160
Ksp22I TGATCA 3 cut(s) 355, 592, 1315
KspAI GTTAAC 1 cut(s) 1513
LguI GCTCTTC 2 cut(s) 41, 760
LmnI GCTCC 1 cut(s) 912
Lsp1109I GCAGC 4 cut(s) 812, 1027, 1174, 1370
LweI GCATC 1 cut(s) 1132
MaeI CTAG 6 cut(s) 30, 581, 1034, 1248, 1610, 1638
MaeII ACGT 5 cut(s) 817, 1008, 1012, 1067, 1394
MaeIII GTNAC 7 cut(s) 408, 542, 569, 722, 813, 1013, 1655
MfeI CAATTG 1 cut(s) 778
MflI RGATCY 2 cut(s) 796, 929
MhlI GDGCHC 3 cut(s) 755, 901, 1215
MlyI GAGTC 4 cut(s) 510, 719, 848, 1719
MmeI TCCRAC 2 cut(s) 648, 1508
MroXI GAANNNNTTC 2 cut(s) 642, 1471
MseI TTAA 6 cut(s) 683, 1064, 1080, 1410, 1493, 1512
MslI CAYNNNNRTG 4 cut(s) 234, 294, 540, 1683
MspCI CTTAAG 1 cut(s) 1409
MspI CCGG 3 cut(s) 375, 455, 1228
MspR9I CCNGG 5 cut(s) 376, 455, 1140, 1149, 1715
MunI CAATTG 1 cut(s) 778
Mva1269I GAATGC 1 cut(s) 89
MvaI CCWGG 3 cut(s) 1140, 1149, 1715
MwoI GCNNNNNNNGC 5 cut(s) 123, 1049, 1149, 1171, 1219
NciI CCSGG 2 cut(s) 376, 455
NlaIV GGNNCC 1 cut(s) 1266
NmuCI GTSAC 7 cut(s) 408, 542, 569, 722, 813, 1013, 1655
NsbI TGCGCA 1 cut(s) 1161
OliI CACNNNNGTG 1 cut(s) 540
PaeR7I CTCGAG 1 cut(s) 1342
PagI TCATGA 2 cut(s) 1318, 1500
PceI AGGCCT 1 cut(s) 1614
PciSI GCTCTTC 2 cut(s) 41, 760
PctI GAATGC 1 cut(s) 89
PdmI GAANNNNTTC 2 cut(s) 642, 1471
PfeI GAWTC 8 cut(s) 337, 422, 587, 920, 978, 1347, 1386, 1736
Pfl23II CGTACG 1 cut(s) 1009
PfoI TCCNGGA 1 cut(s) 1713
PkrI GCNGC 6 cut(s) 128, 660, 827, 1042, 1164, 1360
PleI GAGTC 4 cut(s) 509, 719, 847, 1718
PpsI GAGTC 4 cut(s) 509, 719, 847, 1718
Ppu21I YACGTR 1 cut(s) 818
PshAI GACNNNNGTC 1 cut(s) 1011
PshBI ATTAAT 1 cut(s) 683
Psp124BI GAGCTC 1 cut(s) 755
Psp1406I AACGTT 1 cut(s) 1394
Psp6I CCWGG 3 cut(s) 1138, 1147, 1713
PspFI CCCAGC 1 cut(s) 1756
PspGI CCWGG 3 cut(s) 1138, 1147, 1713
PspLI CGTACG 1 cut(s) 1009
PspN4I GGNNCC 1 cut(s) 1266
PspPI GGNCC 3 cut(s) 507, 1113, 1225
PstI CTGCAG 1 cut(s) 1194
PsuI RGATCY 2 cut(s) 796, 929
RsaI GTAC 4 cut(s) 251, 459, 881, 1011
RsaNI GTAC 4 cut(s) 250, 458, 880, 1010
RseI CAYNNNNRTG 4 cut(s) 234, 294, 540, 1683
SacI GAGCTC 1 cut(s) 755
SapI GCTCTTC 2 cut(s) 41, 760
SaqAI TTAA 6 cut(s) 683, 1064, 1080, 1410, 1493, 1512
SatI GCNGC 6 cut(s) 127, 659, 826, 1041, 1163, 1359
Sau96I GGNCC 3 cut(s) 507, 1113, 1225
ScaI AGTACT 1 cut(s) 251
SchI GAGTC 4 cut(s) 510, 719, 848, 1719
ScrFI CCNGG 5 cut(s) 376, 455, 1140, 1149, 1715
SduI GDGCHC 3 cut(s) 755, 901, 1215
SfaNI GCATC 1 cut(s) 1132
SfcI CTRYAG 1 cut(s) 1190
Sfr274I CTCGAG 1 cut(s) 1342
SinI GGWCC 1 cut(s) 507
SlaI CTCGAG 1 cut(s) 1342
SmiMI CAYNNNNRTG 4 cut(s) 234, 294, 540, 1683
SmlI CTYRAG 5 cut(s) 547, 1342, 1409, 1564, 1660
SmoI CTYRAG 5 cut(s) 547, 1342, 1409, 1564, 1660
SseBI AGGCCT 1 cut(s) 1614
SsiI CCGC 2 cut(s) 126, 659
SspI AATATT 1 cut(s) 138
SspMI CTAG 6 cut(s) 30, 581, 1034, 1248, 1610, 1638
SstI GAGCTC 1 cut(s) 755
StuI AGGCCT 1 cut(s) 1614
StyD4I CCNGG 5 cut(s) 374, 453, 1138, 1147, 1713
TaaI ACNGT 3 cut(s) 733, 850, 1764
TaiI ACGT 5 cut(s) 820, 1011, 1015, 1070, 1397
TaqI TCGA 8 cut(s) 491, 499, 885, 953, 976, 1101, 1294, 1343
TaqII GACCGA 2 cut(s) 330, 806
TatI WGTACW 1 cut(s) 249
TauI GCSGC 2 cut(s) 129, 661
TfiI GAWTC 8 cut(s) 337, 422, 587, 920, 978, 1347, 1386, 1736
Tru1I TTAA 6 cut(s) 683, 1064, 1080, 1410, 1493, 1512
Tru9I TTAA 6 cut(s) 683, 1064, 1080, 1410, 1493, 1512
TscAI CASTG 3 cut(s) 262, 738, 853
TseFI GTSAC 7 cut(s) 408, 542, 569, 722, 813, 1013, 1655
TseI GCWGC 4 cut(s) 825, 1040, 1162, 1358
Tsp45I GTSAC 7 cut(s) 408, 542, 569, 722, 813, 1013, 1655
TspGWI ACGGA 1 cut(s) 46
TspRI CASTG 3 cut(s) 262, 738, 853
Vha464I CTTAAG 1 cut(s) 1409
VpaK11BI GGWCC 1 cut(s) 507
VspI ATTAAT 1 cut(s) 683
XapI RAATTY 3 cut(s) 640, 714, 1097
XbaI TCTAGA 1 cut(s) 580
XhoI CTCGAG 1 cut(s) 1342
XmiI GTMKAC 1 cut(s) 60
XmnI GAANNNNTTC 2 cut(s) 642, 1471
XspI CTAG 6 cut(s) 30, 581, 1034, 1248, 1610, 1638
ZrmI AGTACT 1 cut(s) 251
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.