Rh7DG074500

Protein of unknown function (DUF1666)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0008073
Physical Location & Seq
Reverse (-)
5771204 .. 5774852
3649 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG074500.1

Sequence Viewer

Length: 1506 bp
ATGCCATGTTCAACAGAGGAAGCACTTGCTAGGCTCTTCTACAATACTTCATCCTCCGTCTACCTCCTCCTTCTCTTCCTCTATGTCTCGTCCATTGTTCTTGCCAAGTTCTTCTACTTCGTTGGCAGCAACCCAATATTCCCAAGAAACCAGAATGGATACGAGAATTATCTGTTTTCGGATGAAGAAGAGGAGGAAGAAGAAGAATTAGAAGAAAGGCACAGCTTTCATGTTCATGGTCATCAGTACTACACTGAAAACGATCATCTGGTGGCCGACATCATTCATGGAGGTGAAGCACTAAAGTTTTTGCCTAATGACAACCTTCAGAAGGATCGATCAAGTTTTTCTGATCAAGAGTTCATTAGCGCCGGGGGGAGCCTAATTGAAGAAGACCAAAATGATATGTCACAAGGTTCAGAATCAGAACAAGATCATATTGCAGAGGAAATCCCTGTCAGAGATACTGCTTCTGTTTGCAATTCTGTCCCGAATGATCAAGTCTGGACCACTTCTCCGATCACTATAGAACTACACAGAAATCATAATCATGAGGATTCTGATCAGATTACCGAAAACAATACAGAGACAAAATTATCTGGGGATGAAAATTTTCTAGTATTTAGGCCGCCCCAGTCGGAGACCAAGAAATTAATCAAGGTTCAAGAAAACAAGGAGGATGAAATTTTTGGTGACTCGACAGTTGGATCAACTTCTAAGAGCTCTTCTGAATGGAGAAGCTCAATCAATTGTTCTGCGACCGAAGATCCATTTTCTTCCTCATCACGGAGAAGCTGCCCCAAGTGGGAGTCTTACACTGTCTTTCAGAAATATGATGAAGAAATGATGTACCTAGACAGAATTAGTGCTCAAAAGCTCCATGAAACAGAATCACTTAGGTCTATTCAGGTTTGTCCAAGATCAATTTCAGAGAGGATTGTTCATCGAATCTCATTAGCAACCAAACGGCCATCTGACGTACGTCACAACCCTTATCATGAACTAGAGGCTGCTTATGTTGCTCAAATTTGCATAACGTGGGAAGCACTTAACTGGAACTACAAGAAATTCGAGCAAAAAAGGGCCTCGTCGCATCACGATTTTGACCCTGGCTGTCCTGGCCAGATTGCGCAGCAGTTTCAGCAGTTCCAAGTTCTTTTGCAGAGATATGTAGAGAATGAGCCCTACGAGCAGGGCCGGAGACCTGAGATTTACGCTAGGATGAGGCTTTTGGCACCGAAGCTACTTCAAGTTCCAGAATATCGAGCTTCAGAAGATGATGATCATGAAAAGGAAGAAGGTTTTGGCTCGAGAATCTCCTCAGCTGCGTTTCTAATGATAATGGAAGATGGAATCCGAACGTTCATGGAATTTCTTAAGGCTGATAAAGAGAAACCCTGCCAAAAGTTATCAGCCATGTTTAGGAGAAAACGAAGAGGTTCAGTTGATCCAACTCTTCTAAATCTCATGAAAAAAGTTAACCAAAAGGTAAGTTATATATATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1666

Protein Analysis

501

Amino Acids

58.11

Weight (kDa)

5.11

Isoelectric Point (pI)

60.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1666 PF07891 333 - 496 9.3e-57 Protein of unknown function (DUF1666)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1131
AccB1I GGYRCC 1 cut(s) 1234
AccI GTMKAC 1 cut(s) 60
AciI CCGC 1 cut(s) 629
AclI AACGTT 1 cut(s) 1361
AclWI GGATC 4 cut(s) 342, 715, 761, 1442
AcoI YGGCCR 3 cut(s) 273, 968, 1120
AcsI RAATTY 5 cut(s) 610, 684, 1026, 1067, 1370
AcuI CTGAAG 2 cut(s) 311, 1254
AfaI GTAC 3 cut(s) 248, 851, 981
AfiI CCNNNNNNNGG 4 cut(s) 331, 786, 805, 1422
AflII CTTAAG 1 cut(s) 1376
AgsI TTSAA 4 cut(s) 12, 389, 665, 1250
AjnI CCWGG 2 cut(s) 1108, 1117
AjuI GAANNNNNNNTTGG 4 cut(s) 910, 942, 1287, 1319
AluBI AGCT 8 cut(s) 225, 723, 741, 795, 877, 1243, 1268, 1325
AluI AGCT 8 cut(s) 225, 723, 741, 795, 877, 1243, 1268, 1325
Alw21I GWGCWC 2 cut(s) 725, 871
Alw26I GTCTC 4 cut(s) 91, 581, 635, 1195
AlwI GGATC 4 cut(s) 342, 715, 761, 1442
Ama87I CYCGRG 1 cut(s) 1309
AoxI GGCC 6 cut(s) 273, 626, 968, 1083, 1120, 1195
ApeKI GCWGC 5 cut(s) 126, 795, 1010, 1132, 1325
ApoI RAATTY 5 cut(s) 610, 684, 1026, 1067, 1370
AseI ATTAAT 1 cut(s) 653
Asp700I GAANNNNTTC 2 cut(s) 612, 1438
AspLEI GCGC 2 cut(s) 371, 1132
AspS9I GGNCC 3 cut(s) 507, 1083, 1195
AsuC2I CCSGG 1 cut(s) 373
AsuHPI GGTGA 2 cut(s) 305, 704
AvaI CYCGRG 1 cut(s) 1309
AvaII GGWCC 1 cut(s) 507
BalI TGGCCA 1 cut(s) 1122
BanI GGYRCC 1 cut(s) 1234
BanII GRGCYC 2 cut(s) 725, 1185
BbsI GAAGAC 1 cut(s) 399
Bbv12I GWGCWC 2 cut(s) 725, 871
BbvCI CCTCAGC 1 cut(s) 1321
BbvI GCAGC 5 cut(s) 138, 782, 997, 1144, 1312
BccI CCATC 2 cut(s) 979, 1343
BceAI ACGGC 1 cut(s) 983
BciT130I CCWGG 2 cut(s) 1110, 1119
BciVI GTATCC 1 cut(s) 152
BclI TGATCA 4 cut(s) 352, 496, 562, 1282
BcnI CCSGG 1 cut(s) 373
BcoDI GTCTC 4 cut(s) 91, 581, 635, 1195
BfaI CTAG 5 cut(s) 30, 617, 854, 1004, 1218
BfmI CTRYAG 1 cut(s) 525
BfoI RGCGCY 1 cut(s) 372
BfrI CTTAAG 1 cut(s) 1376
BfuI GTATCC 1 cut(s) 152
BisI GCNGC 6 cut(s) 127, 629, 796, 1011, 1133, 1326
BlsI GCNGC 6 cut(s) 128, 630, 797, 1012, 1134, 1327
BmcAI AGTACT 1 cut(s) 248
Bme1390I CCNGG 3 cut(s) 373, 1110, 1119
Bme18I GGWCC 1 cut(s) 507
BmeT110I CYCGRG 1 cut(s) 1309
BmgT120I GGNCC 3 cut(s) 507, 1083, 1195
BmiI GGNNCC 2 cut(s) 380, 1236
BmrFI CCNGG 3 cut(s) 373, 1110, 1119
BmrI ACTGGG 1 cut(s) 628
BmsI GCATC 1 cut(s) 1102
BmuI ACTGGG 1 cut(s) 628
BoxI GACNNNNGTC 1 cut(s) 981
BpiI GAAGAC 1 cut(s) 399
Bpu10I CCTNAGC 1 cut(s) 1321
BpuMI CCSGG 1 cut(s) 373
Bsa29I ATCGAT 1 cut(s) 337
BsaBI GATNNNNATC 2 cut(s) 561, 1281
BsaI GGTCTC 2 cut(s) 635, 1195
BsaJI CCNNGG 2 cut(s) 372, 1108
BsaXI ACNNNNNCTCC 2 cut(s) 499, 529
Bsc4I CCNNNNNNNGG 4 cut(s) 331, 786, 805, 1422
Bse1I ACTGG 2 cut(s) 634, 1058
Bse8I GATNNNNATC 2 cut(s) 561, 1281
BseBI CCWGG 2 cut(s) 1110, 1119
BseCI ATCGAT 1 cut(s) 337
BseDI CCNNGG 2 cut(s) 372, 1108
BseGI GGATG 5 cut(s) 50, 187, 610, 685, 1227
BseJI GATNNNNATC 2 cut(s) 561, 1281
BseLI CCNNNNNNNGG 4 cut(s) 331, 786, 805, 1422
BseMII CTCAG 2 cut(s) 1197, 1335
BseNI ACTGG 2 cut(s) 634, 1058
BseRI GAGGAG 3 cut(s) 56, 206, 1309
BseXI GCAGC 5 cut(s) 138, 782, 997, 1144, 1312
Bsh1285I CGRYCG 1 cut(s) 762
BshFI GGCC 6 cut(s) 275, 628, 970, 1085, 1122, 1197
BshNI GGYRCC 1 cut(s) 1234
BshVI ATCGAT 1 cut(s) 337
BsiEI CGRYCG 1 cut(s) 762
BsiHKAI GWGCWC 2 cut(s) 725, 871
BsiHKCI CYCGRG 1 cut(s) 1309
BsiSI CCGG 2 cut(s) 372, 1198
BsiWI CGTACG 1 cut(s) 979
BslFI GGGAC 1 cut(s) 473
BslI CCNNNNNNNGG 4 cut(s) 331, 786, 805, 1422
BsmAI GTCTC 4 cut(s) 91, 581, 635, 1195
BsmFI GGGAC 1 cut(s) 473
BsnI GGCC 6 cut(s) 275, 628, 970, 1085, 1122, 1197
Bso31I GGTCTC 2 cut(s) 635, 1195
BsoBI CYCGRG 1 cut(s) 1309
Bsp1286I GDGCHC 3 cut(s) 725, 871, 1185
BspACI CCGC 1 cut(s) 629
BspANI GGCC 6 cut(s) 275, 628, 970, 1085, 1122, 1197
BspCNI CTCAG 2 cut(s) 1198, 1334
BspDI ATCGAT 1 cut(s) 337
BspHI TCATGA 4 cut(s) 550, 997, 1285, 1467
BspLI GGNNCC 2 cut(s) 380, 1236
BspPI GGATC 4 cut(s) 342, 715, 761, 1442
BspQI GCTCTTC 2 cut(s) 41, 730
BspT107I GGYRCC 1 cut(s) 1234
BspTI CTTAAG 1 cut(s) 1376
BspTNI GGTCTC 2 cut(s) 635, 1195
BsrI ACTGG 2 cut(s) 634, 1058
BssECI CCNNGG 2 cut(s) 372, 1108
Bst2UI CCWGG 2 cut(s) 1110, 1119
Bst4CI ACNGT 2 cut(s) 703, 820
Bst6I CTCTTC 6 cut(s) 41, 80, 183, 730, 1429, 1461
BstAFI CTTAAG 1 cut(s) 1376
BstDEI CTNAG 4 cut(s) 717, 896, 1206, 1321
BstENI CCTNNNNNAGG 1 cut(s) 329
BstF5I GGATG 5 cut(s) 50, 187, 610, 685, 1227
BstH2I RGCGCY 1 cut(s) 372
BstHHI GCGC 2 cut(s) 371, 1132
BstMAI GTCTC 4 cut(s) 91, 581, 635, 1195
BstMCI CGRYCG 1 cut(s) 762
BstMWI GCNNNNNNNGC 4 cut(s) 1019, 1119, 1141, 1189
BstNI CCWGG 2 cut(s) 1110, 1119
BstPAI GACNNNNGTC 1 cut(s) 981
BstSCI CCNGG 3 cut(s) 371, 1108, 1117
BstSFI CTRYAG 1 cut(s) 525
BstV1I GCAGC 5 cut(s) 138, 782, 997, 1144, 1312
BstV2I GAAGAC 1 cut(s) 399
BstX2I RGATCY 1 cut(s) 766
BstYI RGATCY 1 cut(s) 766
Bsu15I ATCGAT 1 cut(s) 337
BsuI GTATCC 1 cut(s) 152
BsuRI GGCC 6 cut(s) 275, 628, 970, 1085, 1122, 1197
BsuTUI ATCGAT 1 cut(s) 337
BtsCI GGATG 5 cut(s) 50, 187, 610, 685, 1227
BtsIMutI CAGTG 2 cut(s) 252, 816
CciI TCATGA 4 cut(s) 550, 997, 1285, 1467
CfoI GCGC 2 cut(s) 371, 1132
Cfr13I GGNCC 3 cut(s) 507, 1083, 1195
ClaI ATCGAT 1 cut(s) 337
Csp6I GTAC 3 cut(s) 247, 850, 980
CviQI GTAC 3 cut(s) 247, 850, 980
DdeI CTNAG 4 cut(s) 717, 896, 1206, 1321
EaeI YGGCCR 3 cut(s) 273, 968, 1120
Eam1104I CTCTTC 6 cut(s) 41, 80, 183, 730, 1429, 1461
EarI CTCTTC 6 cut(s) 41, 80, 183, 730, 1429, 1461
Ecl136II GAGCTC 1 cut(s) 723
Eco24I GRGCYC 2 cut(s) 725, 1185
Eco31I GGTCTC 2 cut(s) 635, 1195
Eco47I GGWCC 1 cut(s) 507
Eco53kI GAGCTC 1 cut(s) 723
Eco57I CTGAAG 2 cut(s) 311, 1254
Eco88I CYCGRG 1 cut(s) 1309
EcoICRI GAGCTC 1 cut(s) 723
EcoNI CCTNNNNNAGG 1 cut(s) 329
EcoO109I RGGNCCY 1 cut(s) 1083
EcoRII CCWGG 2 cut(s) 1108, 1117
EcoT38I GRGCYC 2 cut(s) 725, 1185
FaqI GGGAC 1 cut(s) 473
FbaI TGATCA 4 cut(s) 352, 496, 562, 1282
FblI GTMKAC 1 cut(s) 60
Fnu4HI GCNGC 6 cut(s) 127, 629, 796, 1011, 1133, 1326
FokI GGATG 5 cut(s) 37, 194, 617, 692, 1234
FriOI GRGCYC 2 cut(s) 725, 1185
Fsp4HI GCNGC 6 cut(s) 127, 629, 796, 1011, 1133, 1326
FspBI CTAG 5 cut(s) 30, 617, 854, 1004, 1218
FspI TGCGCA 1 cut(s) 1131
GlaI GCGC 2 cut(s) 370, 1131
GluI GCNGC 6 cut(s) 127, 629, 796, 1011, 1133, 1326
HaeII RGCGCY 1 cut(s) 372
HaeIII GGCC 6 cut(s) 275, 628, 970, 1085, 1122, 1197
HapII CCGG 2 cut(s) 372, 1198
HhaI GCGC 2 cut(s) 371, 1132
Hin6I GCGC 2 cut(s) 369, 1130
HinP1I GCGC 2 cut(s) 369, 1130
HincII GTYRAC 1 cut(s) 1480
HindII GTYRAC 1 cut(s) 1480
HinfI GANTC 8 cut(s) 422, 557, 695, 809, 890, 948, 1314, 1353
HpaI GTTAAC 1 cut(s) 1480
HpaII CCGG 2 cut(s) 372, 1198
HphI GGTGA 2 cut(s) 305, 704
Hpy166II GTNNAC 2 cut(s) 61, 1480
Hpy8I GTNNAC 2 cut(s) 61, 1480
Hpy99I CGWCG 1 cut(s) 1093
HpyAV CCTTC 4 cut(s) 80, 325, 335, 1292
HpyCH4III ACNGT 2 cut(s) 703, 820
HpyCH4IV ACGT 4 cut(s) 978, 982, 1037, 1361
HpyCH4V TGCA 4 cut(s) 443, 480, 1032, 1162
HpyF10VI GCNNNNNNNGC 4 cut(s) 1019, 1119, 1141, 1189
HpyF3I CTNAG 4 cut(s) 717, 896, 1206, 1321
HpySE526I ACGT 4 cut(s) 978, 982, 1037, 1361
HspAI GCGC 2 cut(s) 369, 1130
Ksp22I TGATCA 4 cut(s) 352, 496, 562, 1282
KspAI GTTAAC 1 cut(s) 1480
LguI GCTCTTC 2 cut(s) 41, 730
LmnI GCTCC 2 cut(s) 378, 882
Lsp1109I GCAGC 5 cut(s) 138, 782, 997, 1144, 1312
LweI GCATC 1 cut(s) 1102
MaeI CTAG 5 cut(s) 30, 617, 854, 1004, 1218
MaeII ACGT 4 cut(s) 978, 982, 1037, 1361
MaeIII GTNAC 3 cut(s) 408, 692, 983
MfeI CAATTG 1 cut(s) 748
MflI RGATCY 1 cut(s) 766
MhlI GDGCHC 3 cut(s) 725, 871, 1185
MlsI TGGCCA 1 cut(s) 1122
MluNI TGGCCA 1 cut(s) 1122
MlyI GAGTC 2 cut(s) 689, 818
MmeI TCCRAC 3 cut(s) 618, 685, 1475
Mox20I TGGCCA 1 cut(s) 1122
MroXI GAANNNNTTC 2 cut(s) 612, 1438
MscI TGGCCA 1 cut(s) 1122
MseI TTAA 5 cut(s) 653, 1050, 1377, 1479, 1504
MslI CAYNNNNRTG 3 cut(s) 234, 291, 549
Msp20I TGGCCA 1 cut(s) 1122
MspA1I CMGCKG 1 cut(s) 1325
MspCI CTTAAG 1 cut(s) 1376
MspI CCGG 2 cut(s) 372, 1198
MspR9I CCNGG 3 cut(s) 373, 1110, 1119
MunI CAATTG 1 cut(s) 748
MvaI CCWGG 2 cut(s) 1110, 1119
MwoI GCNNNNNNNGC 4 cut(s) 1019, 1119, 1141, 1189
NciI CCSGG 1 cut(s) 373
NlaIV GGNNCC 2 cut(s) 380, 1236
NmuCI GTSAC 3 cut(s) 408, 692, 983
NsbI TGCGCA 1 cut(s) 1131
PaeR7I CTCGAG 1 cut(s) 1309
PagI TCATGA 4 cut(s) 550, 997, 1285, 1467
PciSI GCTCTTC 2 cut(s) 41, 730
PdmI GAANNNNTTC 2 cut(s) 612, 1438
PfeI GAWTC 6 cut(s) 422, 557, 890, 948, 1314, 1353
Pfl23II CGTACG 1 cut(s) 979
PkrI GCNGC 6 cut(s) 128, 630, 797, 1012, 1134, 1327
PleI GAGTC 2 cut(s) 689, 817
PpsI GAGTC 2 cut(s) 689, 817
PshAI GACNNNNGTC 1 cut(s) 981
PshBI ATTAAT 1 cut(s) 653
Psp124BI GAGCTC 1 cut(s) 725
Psp1406I AACGTT 1 cut(s) 1361
Psp6I CCWGG 2 cut(s) 1108, 1117
PspGI CCWGG 2 cut(s) 1108, 1117
PspLI CGTACG 1 cut(s) 979
PspN4I GGNNCC 2 cut(s) 380, 1236
PspPI GGNCC 3 cut(s) 507, 1083, 1195
PsuI RGATCY 1 cut(s) 766
PvuII CAGCTG 1 cut(s) 1325
RsaI GTAC 3 cut(s) 248, 851, 981
RsaNI GTAC 3 cut(s) 247, 850, 980
RseI CAYNNNNRTG 3 cut(s) 234, 291, 549
SacI GAGCTC 1 cut(s) 725
SapI GCTCTTC 2 cut(s) 41, 730
SaqAI TTAA 5 cut(s) 653, 1050, 1377, 1479, 1504
SatI GCNGC 6 cut(s) 127, 629, 796, 1011, 1133, 1326
Sau96I GGNCC 3 cut(s) 507, 1083, 1195
ScaI AGTACT 1 cut(s) 248
SchI GAGTC 2 cut(s) 689, 818
ScrFI CCNGG 3 cut(s) 373, 1110, 1119
SduI GDGCHC 3 cut(s) 725, 871, 1185
SfaNI GCATC 1 cut(s) 1102
SfcI CTRYAG 1 cut(s) 525
Sfr274I CTCGAG 1 cut(s) 1309
SinI GGWCC 1 cut(s) 507
SlaI CTCGAG 1 cut(s) 1309
SmiMI CAYNNNNRTG 3 cut(s) 234, 291, 549
SmlI CTYRAG 2 cut(s) 1309, 1376
SmoI CTYRAG 2 cut(s) 1309, 1376
SsiI CCGC 1 cut(s) 629
SspI AATATT 1 cut(s) 138
SspMI CTAG 5 cut(s) 30, 617, 854, 1004, 1218
SstI GAGCTC 1 cut(s) 725
StyD4I CCNGG 3 cut(s) 371, 1108, 1117
TaaI ACNGT 2 cut(s) 703, 820
TaiI ACGT 4 cut(s) 981, 985, 1040, 1364
TaqI TCGA 6 cut(s) 337, 698, 946, 1071, 1264, 1310
TaqII GACCGA 1 cut(s) 776
TatI WGTACW 1 cut(s) 246
TauI GCSGC 1 cut(s) 631
TfiI GAWTC 6 cut(s) 422, 557, 890, 948, 1314, 1353
Tru1I TTAA 5 cut(s) 653, 1050, 1377, 1479, 1504
Tru9I TTAA 5 cut(s) 653, 1050, 1377, 1479, 1504
TscAI CASTG 2 cut(s) 259, 823
TseFI GTSAC 3 cut(s) 408, 692, 983
TseI GCWGC 5 cut(s) 126, 795, 1010, 1132, 1325
Tsp45I GTSAC 3 cut(s) 408, 692, 983
TspGWI ACGGA 2 cut(s) 46, 802
TspRI CASTG 2 cut(s) 259, 823
Vha464I CTTAAG 1 cut(s) 1376
VpaK11BI GGWCC 1 cut(s) 507
VspI ATTAAT 1 cut(s) 653
XagI CCTNNNNNAGG 1 cut(s) 329
XapI RAATTY 5 cut(s) 610, 684, 1026, 1067, 1370
XhoI CTCGAG 1 cut(s) 1309
XmiI GTMKAC 1 cut(s) 60
XmnI GAANNNNTTC 2 cut(s) 612, 1438
XspI CTAG 5 cut(s) 30, 617, 854, 1004, 1218
ZrmI AGTACT 1 cut(s) 248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.